MYL2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYL2
Entrez Gene ID	4633
Full Name	myosin light chain 2
Synonyms	CMH10,MLC-2s/v,MLC2
General protein information	Preferred Names myosin light chain 2 Names myosin regulatory light chain 2, ventricular/cardiac muscle isoform MLC-2 MLC-2v RLC of myosin cardiac myosin light chain 2 cardiac ventricular myosin light chain 2 myosin light chain 2, slow skeletal/ventricular muscle isoform myosin, light chain 2, regulatory, cardiac, slow myosin, light polypeptide 2, regulatory, cardiac, slow regulatory light chain of myosin slow cardiac myosin regulatory light chain 2 ventricular myosin light chain 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q24.11
Summary	Thus gene encodes the regulatory light chain associated with cardiac myosin beta (or slow) heavy chain. Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in this gene are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. [provided by RefSeq, Jul 2008].
Disorder MIM:	160781
Disorder Html:	Cardiomyopathy, hypertrophic, 10, 608758 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000432.4	NP_000423.2	myosin regulatory light chain 2, ventricular/cardiac muscle isoform
NM_000432.3	NP_000423.2	myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Myl2	NP_001030329.2
Gallus gallus (chicken)	MYL2	NP_001258858.1
Canis lupus familiaris (dog)	MYL2	NP_001003069.1
Danio rerio (zebrafish)	myl2b	NP_001035134.1
Danio rerio (zebrafish)	LOC100537244	XP_003198611.2
Xenopus tropicalis (tropical clawed frog)	myl2	NP_001107717.1
Pan troglodytes (chimpanzee)	MYL2	XP_001144828.2
Mus musculus (house mouse)	Myl2	NP_034991.3
Bos taurus (cattle)	MYL2	NP_001030197.1
Homo sapiens (human)	MYL2	NP_000423.2
	MYL2	XP_001100796.1

Gene Ontology
Bibliography

Related articles in PubMed

Isolation and characterization of ventricular-like cells derived from NKX2-5
Yamauchi K, Li J, Morikawa K, Liu L, Shirayoshi Y, Nakatsuji N, Elliott DA, Hisatome I, Suemori H
Biochemical and biophysical research communications495(1)1278-1284(2018 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

NKX2-5 and MLC2v double-positive cells possess ventricular-like properties. The results demonstrate that the NKX2-5(eGFP/w) and MLC2v(mCherry/w) hPSCs provide a powerful model system to capture region-specific cardiac differentiation from early to late stages. Our study would facilitate subtype-specific cardiac development and functional analysis using the hPSC-derived sources.
Title: Isolation and characterization of ventricular-like cells derived from NKX2-5

Structural dynamics-based approach reveals that the E56G mutation in human ventricular essential light chain affects the structure of the actin-myosin complex in the presence of ATP. The mutation increases the population in the S structural state (increasing the duty ratio), and changes the structure of the W state, so that it more closely resembles the S state.
Title: A Cardiomyopathy Mutation in the Myosin Essential Light Chain Alters Actomyosin Structure.

Lipolysis-stimulated lipoprotein receptors (LSRs) localized to bicellular junctions in association with myosin regulatory light chain 2 (MRLC2) at low cell densities and to tricellular contacts when myosin phosphatase target subunit 1 (MYPT1) localized to the bicellular regions.
Title: The bicellular tensile force sorts the localization of LSRs in bicellular and tricellular junctions.

Mutation in myosin regulatory light chain gene is associated with defective myosin motor function that ultimately result in pathological hypertrophic remodeling.
Title: Cardiac contractility, motor function, and cross-bridge kinetics in N47K-RLC mutant mice.

Two siblings with hypertrophic cardiomyopathy had the pathogenic variant p.Ala13Thr variant in MYL2.
Title: A Potential Oligogenic Etiology of Hypertrophic Cardiomyopathy: A Classic Single-Gene Disorder.

The following MYL2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYL2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18354	NM_000432.4 Latest version!	Homo sapiens myosin light chain 2 (MYL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18354	NM_000432.3	Homo sapiens myosin light chain 2 (MYL2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYL2 ( NM_000432.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000423.2 Homo sapiens myosin light chain 2 (MYL2), mRNA.

Price & Availability↑

CloneID	OHu18354
Clone ID Related Accession (Same CDS sequence)	NM_000432.4 , NM_000432.3
Accession Version	NM_000432.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC015821.1. On Nov 30, 2019 this sequence version replaced NM_000432.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC031008.1, BU193625.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG 
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC 
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT 
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC 
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG 
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC 
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC 
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000423.2
CDS	51..551
Translation	MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

Target ORF information:

RefSeq Version	NM_000432.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 2 (MYL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000432.4

ORF Insert Sequence:

ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG 
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC 
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT 
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC 
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG 
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC 
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC 
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYL2 ( NM_000432.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000423.2 Homo sapiens myosin light chain 2 (MYL2), mRNA.

Price & Availability↑

CloneID	OHu18354
Clone ID Related Accession (Same CDS sequence)	NM_000432.4 , NM_000432.3
Accession Version	NM_000432.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-25
Organism	Homo sapiens(human)
Product	myosin regulatory light chain 2, ventricular/cardiac muscle isoform
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA090235.1, BC015821.1 and BC031008.1. This sequence is a reference standard in the RefSeqGene project. On Jun 14, 2008 this sequence version replaced NM_000432.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279847.4406.1, BC031008.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151741, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG 
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC 
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT 
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC 
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG 
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC 
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC 
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000423.2
CDS	72..572
Translation	MAPKKAKKRAGGANSNVFSMFEQTQIQEFKEAFTIMDQNRDGFIDKNDLRDTFAALGRVNVKNEEIDEMIKEAPGPINFTVFLTMFGEKLKGADPEETILNAFKVFDPEGKGVLKADYVREMLTTQAERFSKEEVDQMFAAFPPDVTGNLDYKNLVHIITHGEEKD

Target ORF information:

RefSeq Version	NM_000432.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin light chain 2 (MYL2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000432.3

ORF Insert Sequence:

ATGGCACCTA AGAAAGCAAA GAAGAGAGCC GGGGGCGCCA ACTCCAACGT GTTCTCCATG 
TTCGAACAGA CCCAAATCCA GGAATTTAAG GAGGCCTTCA CTATCATGGA CCAGAACAGG 
GATGGCTTCA TTGACAAGAA CGATCTGAGA GACACCTTTG CTGCCCTTGG GCGAGTGAAC 
GTGAAAAATG AAGAAATTGA TGAAATGATC AAGGAGGCTC CGGGTCCAAT TAACTTTACT 
GTGTTCCTCA CAATGTTTGG GGAGAAACTT AAGGGAGCGG ACCCTGAGGA AACCATTCTC 
AACGCATTCA AAGTGTTTGA CCCTGAAGGC AAAGGGGTGC TGAAGGCTGA TTACGTTCGG 
GAAATGCTGA CCACGCAGGC GGAGAGGTTT TCCAAGGAGG AGGTTGACCA GATGTTCGCC 
GCCTTCCCCC CTGACGTGAC TGGCAACTTG GACTACAAGA ACCTGGTGCA CATCATCACC 
CACGGAGAAG AGAAGGACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Isolation and characterization of ventricular-like cells derived from NKX2-5
Biochemical and biophysical research communications495(1)1278-1284(2018 01)
Yamauchi K,Li J,Morikawa K,Liu L,Shirayoshi Y,Nakatsuji N,Elliott DA,Hisatome I,Suemori H

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MYL2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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