MYH3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH3
Entrez Gene ID	4621
Full Name	myosin heavy chain 3
Synonyms	DA2A,DA2B,DA8,HEMHC,MYHC-EMB,MYHSE1,SMHCE
General protein information	Preferred Names myosin heavy chain 3 Names myosin-3 myosin heavy chain, fast skeletal muscle, embryonic myosin, heavy chain 3, skeletal muscle, embryonic myosin, heavy polypeptide 3, skeletal muscle, embryonic myosin, skeletal, heavy chain, embryonic 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	160720
Disorder Html:	Arthrogryposis, distal, type 2A, 193700 (3); Arthrogryposis, distal, type 2B, 601680 (3); Arthrogryposis, distal, type 8, 178110 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011523870.3	XP_011522172.1	myosin-3 isoform X1
XM_011523871.1	XP_011522173.1	myosin-3 isoform X1
NM_002470.4	NP_002461.2	myosin-3
XM_011523870.1	XP_011522172.1	myosin-3 isoform X1
NM_002470.3	NP_002461.2	myosin-3
XM_011523870.2	XP_011522172.1	myosin-3 isoform X1
XM_011523871.2	XP_011522173.1	myosin-3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	MYH3	NP_002461.2
Pan troglodytes (chimpanzee)	MYH3	XP_003315696.1
Canis lupus familiaris (dog)	MYH3	NP_001107184.1
Mus musculus (house mouse)	Myh3	NP_001093105.1
	MYH3	XP_001114005.2
Rattus norvegicus (Norway rat)	Myh3	NP_036736.1
Bos taurus (cattle)	MYH3	NP_001095305.1
Xenopus tropicalis (tropical clawed frog)	LOC100495065	XP_002937051.2

Gene Ontology
Bibliography

Related articles in PubMed

A novel pathogenic MYH3 mutation in a child with Sheldon-Hall syndrome and vertebral fusions.
Scala M, Accogli A, De Grandis E, Allegri A, Bagowski CP, Shoukier M, Maghnie M, Capra V
American journal of medical genetics. Part A(2018 Jan)

A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGF? signaling and cause autosomal dominant spondylocarpotarsal synostosis.
Zieba J, Zhang W, Chong JX, Forlenza KN, Martin JH, Heard K, Grange DK, Butler MG, Kleefstra T, Lachman RS, Nickerson D, Regnier M, Cohn DH, Bamshad M, Krakow D
Scientific reports741803(2017 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Protein-altering variants of MYH3 were identified in two families with symptoms related to autosomal dominant spondylocarpotarsal synostosis syndrome.
Title: Protein-altering MYH3 variants are associated with a spectrum of phenotypes extending to spondylocarpotarsal synostosis syndrome.

our patient is the first reported case of a child with classical FSS, caused by a common MYH3 mutation, who has an unaffected mother with molecularly proven somatic mosaicism, who is also a likely gonadal mosaic. This case emphasizes the importance of parental genetic testing, when a clinically apparent de novo diagnosis is suspected in a child.
Title: Molecularly proven mosaicism in phenotypically normal parent of a girl with Freeman-Sheldon Syndrome caused by a pathogenic MYH3 mutation.

MYH3 mutations are associated with Freeman-Sheldon Syndrome.
Title: The Most Prevalent Freeman-Sheldon Syndrome Mutations in the Embryonic Myosin Motor Share Functional Defects.

The phenotypic overlap among persons with MPS, coupled with physical findings distinct from other conditions caused by mutations in MYH3.
Title: Autosomal-Dominant Multiple Pterygium Syndrome Is Caused by Mutations in MYH3.

developmental p.Thr178Ile MYH3 myopathy is associated with a combined pathomechanism of insufficient dosage of functional embryonic MyHC and production of mutant protein
Title: Developmental MYH3 Myopathy Associated with Expression of Mutant Protein and Reduced Expression Levels of Embryonic MyHC.

The following MYH3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21221	NM_002470.4 Latest version!	Homo sapiens myosin heavy chain 3 (MYH3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu21221	XM_011523870.3 Latest version!	Homo sapiens myosin heavy chain 3 (MYH3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu21221	XM_011523871.2 Latest version!	Homo sapiens myosin heavy chain 3 (MYH3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
View Old Accession Versions >>
OHu21221	XM_011523870.1	Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu21221	NM_002470.3	Homo sapiens myosin heavy chain 3 (MYH3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu21221	XM_011523870.2	Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu21221	XM_011523871.1	Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH3 ( NM_002470.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002461.2 Homo sapiens myosin heavy chain 3 (MYH3), mRNA.

Price & Availability↑

CloneID	OHu21221
Clone ID Related Accession (Same CDS sequence)	XM_011523870.3 , XM_011523871.1 , NM_002470.4 , XM_011523870.1 , NM_002470.3 , XM_011523870.2 , XM_011523871.2
Accession Version	NM_002470.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	myosin-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP233220.1, BQ956249.1, X13988.1, AC002347.3, BX500019.1, BY796597.2, BP232269.1, BP232093.1, AY517555.1, CD614924.1 and BP370003.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002470.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X13988.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000583535.6/ ENSP00000464317.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002461.2
CDS	89..5911
Translation	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	NM_002470.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 3 (MYH3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002470.4

ORF Insert Sequence:

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH3 ( NM_002470.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002461.2 Homo sapiens myosin heavy chain 3 (MYH3), mRNA.

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CloneID	OHu21221
Clone ID Related Accession (Same CDS sequence)	XM_011523870.3 , XM_011523871.1 , NM_002470.4 , XM_011523870.1 , NM_002470.3 , XM_011523870.2 , XM_011523871.2
Accession Version	NM_002470.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-01
Organism	Homo sapiens(human)
Product	myosin-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP233220.1, BQ956249.1, X13988.1, AC002347.3, BX500019.1, BY796597.2, BP232269.1, BP232093.1, AY517555.1, CD614924.1 and BP370003.1. This sequence is a reference standard in the RefSeqGene project. On Sep 9, 2011 this sequence version replaced NM_002470.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X13988.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002461.2
CDS	89..5911
Translation	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	NM_002470.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 3 (MYH3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002470.3

ORF Insert Sequence:

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH3 ( XM_011523870.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522172.1 Homo sapiens myosin heavy chain 3 (MYH3), transcript variant X1, mRNA.

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CloneID	OHu21221
Clone ID Related Accession (Same CDS sequence)	XM_011523870.3 , XM_011523871.1 , NM_002470.4 , XM_011523870.1 , NM_002470.3 , XM_011523870.2 , XM_011523871.2
Accession Version	XM_011523870.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011523870.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522172.1
CDS	146..5968
Translation	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	XM_011523870.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 3 (MYH3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523870.3

ORF Insert Sequence:

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH3 ( XM_011523871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522173.1 Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X2, mRNA.

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CloneID	OHu21221
Clone ID Related Accession (Same CDS sequence)	XM_011523870.3 , XM_011523871.1 , NM_002470.4 , XM_011523870.1 , NM_002470.3 , XM_011523870.2 , XM_011523871.2
Accession Version	XM_011523871.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	myosin-3 isoform X1
Comment

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011522173.1

Translation

MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	XM_011523871.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523871.1

ORF Insert Sequence:

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH3 ( XM_011523870.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522172.1 Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X1, mRNA.

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CloneID	OHu21221
Clone ID Related Accession (Same CDS sequence)	XM_011523870.3 , XM_011523871.1 , NM_002470.4 , XM_011523870.1 , NM_002470.3 , XM_011523870.2 , XM_011523871.2
Accession Version	XM_011523870.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	myosin-3 isoform X1
Comment

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011522172.1

Translation

MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	XM_011523870.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523870.1

ORF Insert Sequence:

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH3 ( XM_011523870.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522172.1 Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X1, mRNA.

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CloneID	OHu21221
Clone ID Related Accession (Same CDS sequence)	XM_011523870.3 , XM_011523871.1 , NM_002470.4 , XM_011523870.1 , NM_002470.3 , XM_011523870.2 , XM_011523871.2
Accession Version	XM_011523870.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	myosin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523870.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522172.1
CDS	1456..7278
Translation	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	XM_011523870.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523870.2

ORF Insert Sequence:

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH3 ( XM_011523871.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011522173.1 Homo sapiens myosin heavy chain 3 (MYH3), transcript variant X2, mRNA.

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CloneID	OHu21221
Clone ID Related Accession (Same CDS sequence)	XM_011523870.3 , XM_011523871.1 , NM_002470.4 , XM_011523870.1 , NM_002470.3 , XM_011523870.2 , XM_011523871.2
Accession Version	XM_011523871.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myosin-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523871.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011522173.1
CDS	89..5911
Translation	MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTLVVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVVEGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKDSKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKAERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGLYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQVHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADADSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVLEGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLIKAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLERNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELEEEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMVAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEARGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNALAHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDSEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEASLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKADIQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRNEAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRANLLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRIRELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQANAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE

Target ORF information:

RefSeq Version	XM_011523871.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 3 (MYH3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523871.2

ORF Insert Sequence:

ATGAGTAGTG ACACTGAAAT GGAAGTGTTC GGCATAGCTG CTCCTTTCCT CCGGAAGTCA 
GAAAAGGAGA GGATCGAGGC TCAGAACCAG CCCTTTGATG CCAAGACGTA TTGCTTCGTG 
GTGGACTCAA AGGAAGAATA TGCCAAGGGG AAAATCAAGA GTTCTCAGGA TGGGAAGGTC 
ACTGTGGAAA CTGAGGACAA CAGGACCCTG GTGGTCAAAC CAGAGGATGT GTACGCCATG 
AACCCCCCCA AGTTCGACAG GATCGAAGAC ATGGCCATGC TGACGCACCT GAATGAGCCA 
GCCGTGCTGT ACAACCTGAA GGACCGTTAC ACATCTTGGA TGATCTATAC CTACTCAGGC 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCGG TGTACAACCC CGAGGTGGTG 
GAAGGCTACC GAGGCAAAAA GCGCCAGGAG GCCCCACCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGATCGT GAAAACCAGT CCATTCTGAT CACCGGAGAA 
TCCGGGGCAG GAAAGACTGT GAACACCAAA CGGGTCATCC AGTACTTTGC AACAATTGCA 
GCTACTGGGG ACCTGGCCAA GAAGAAGGAC TCCAAAATGA AGGGGACTCT GGAAGATCAA 
ATCATCAGTG CCAATCCCCT GCTGGAGGCC TTTGGGAACG CCAAGACTGT GAGGAATGAC 
AACTCCTCCC GTTTTGGCAA GTTCATCCGA ATCCATTTTG GAACCACTGG GAAGCTGGCC 
TCTGCAGATA TTGAAACTTA TCTTCTGGAA AAATCAAGAG TCACTTTCCA GCTGAAGGCT 
GAAAGAAGCT ACCACATCTT CTACCAGATT CTTTCTAACA AGAAGCCTGA GCTCATAGAG 
CTGCTGCTTA TTACGACCAA CCCTTACGAC TACCCGTTCA TTAGCCAGGG GGAGATCCTG 
GTGGCCAGCA TAGATGATGC AGAGGAGCTG CTGGCTACAG ACAGCGCCAT TGACATCCTG 
GGCTTCACCC CAGAAGAGAA ATCTGGGCTC TACAAGCTGA CGGGAGCCGT GATGCACTAC 
GGGAACATGA AGTTCAAGCA GAAGCAGCGA GAGGAGCAGG CCGAGCCGGA TGGCACAGAA 
GTGGCTGACA AAACAGCCTA TCTGATGGGC CTGAACTCTT CGGACCTCCT AAAAGCTTTG 
TGCTTTCCTA GAGTGAAAGT TGGGAATGAG TACGTTACCA AAGGTCAAAC TGTGGATCAG 
GTTCACCATG CTGTGAATGC TCTTTCAAAA TCAGTTTATG AAAAGTTGTT CTTGTGGATG 
GTCACTCGCA TTAACCAGCA ACTGGATACG AAGCTTCCAA GACAACACTT CATTGGTGTT 
TTGGACATTG CAGGCTTTGA AATCTTTGAG TATAACAGCC TGGAGCAGCT GTGCATCAAC 
TTCACCAATG AGAAACTGCA ACAGTTTTTC AACCACCACA TGTTCGTGCT GGAGCAGGAG 
GAGTACAAGA AGGAAGGCAT CGAGTGGACG TTCATTGACT TCGGGATGGA CCTGGCTGCC 
TGCATCGAGC TCATCGAGAA GCCTATGGGC ATCTTCTCCA TCCTGGAAGA GGAGTGCATG 
TTCCCCAAGG CAACAGACAC CTCCTTCAAG AACAAGCTGT ATGACCAGCA TCTTGGAAAG 
TCCAACAACT TCCAGAAGCC CAAGGTGGTC AAAGGCAGGG CCGAGGCTCA CTTCTCACTG 
ATCCACTATG CGGGCACCGT GGACTACAGT GTCTCAGGTT GGCTGGAGAA GAACAAGGAC 
CCTCTGAACG AGACTGTGGT TGGGCTGTAC CAGAAGTCTT CCAACAGGCT CCTGGCACAC 
CTCTATGCCA CGTTTGCCAC GGCGGATGCT GACAGTGGAA AGAAGAAAGT TGCCAAGAAG 
AAGGGTTCTT CCTTCCAAAC TGTCTCTGCC CTTTTCAGGG AAAACCTGAA CAAGCTGATG 
TCAAATTTAA GAACTACTCA CCCTCATTTT GTGCGTTGTA TAATTCCCAA TGAAACCAAA 
ACTCCAGGGG CTATGGAACA CAGCCTTGTT CTGCACCAGC TGCGGTGTAA CGGTGTCCTG 
GAGGGCATCC GCATCTGCAG GAAAGGGTTC CCAAACAGGA TTCTCTATGG CGATTTTAAA 
CAAAGATACC GAGTGCTGAA TGCCAGTGCA ATCCCTGAGG GACAATTCAT TGACAGCAAG 
AAAGCCTGTG AAAAGCTTCT GGCATCCATT GATATTGACC ACACTCAGTA CAAATTTGGA 
CATACCAAGG TGTTCTTCAA GGCTGGCTTG CTGGGAACCC TGGAAGAGAT GCGGGATGAC 
CGCCTGGCCA AACTAATCAC CCGGACACAA GCTGTGTGCA GAGGGTTCCT CATGCGTGTG 
GAATTCCAGA AGATGGTGCA GAGGAGGGAG TCCATCTTCT GCATCCAGTA CAACATTCGC 
TCATTCATGA ACGTCAAGCA CTGGCCCTGG ATGAAACTCT TCTTCAAGAT CAAGCCCCTC 
CTCAAGAGTG CAGAGACTGA GAAAGAGATG GCCACCATGA AGGAAGAATT CCAGAAAACC 
AAAGATGAAC TCGCCAAGTC GGAGGCAAAA AGGAAGGAGC TAGAGGAAAA ACTGGTGACT 
CTGGTCCAAG AGAAGAATGA CCTGCAGCTC CAAGTACAAG CTGAAAGCGA AAATTTGTTG 
GATGCTGAGG AAAGATGCGA TCAGCTGATC AAAGCCAAAT TCCAGCTCGA GGCCAAGATC 
AAGGAGGTGA CAGAGAGAGC TGAAGATGAG GAGGAGATCA ATGCTGAGCT GACGGCCAAG 
AAGAGGAAAC TGGAGGATGA ATGCTCAGAG CTCAAGAAAG ACATTGATGA CCTTGAGTTG 
ACCCTGGCCA AGGTTGAGAA GGAGAAGCAT GCCACAGAGA ACAAGGTTAA AAACCTTACT 
GAGGAACTCT CTGGGTTAGA TGAAACAATT GCAAAGTTAA CCAGAGAGAA GAAGGCCCTC 
CAAGAGGCGC ACCAGCAGGC CTTGGATGAC CTCCAAGCTG AAGAAGACAA AGTCAATTCT 
TTGAACAAAA CCAAGAGCAA ACTGGAACAG CAAGTGGAAG ACCTGGAAAG CTCCCTAGAA 
CAAGAAAAGA AGCTCCGAGT AGACCTGGAA AGGAACAAAA GGAAATTGGA AGGAGACTTG 
AAGCTTGCTC AAGAGTCCAT ATTAGATCTG GAGAATGACA AGCAACAGCT GGACGAAAGG 
CTCAAGAAGA AAGATTTTGA ATATTGTCAA CTTCAAAGCA AAGTGGAAGA TGAGCAGACA 
CTGGGCCTCC AGTTTCAGAA GAAAATCAAA GAGTTGCAGG CTCGAATTGA GGAGCTGGAA 
GAGGAGATAG AGGCGGAGAG GGCCACCCGC GCGAAGACAG AGAAACAGCG CAGCGACTAT 
GCCCGGGAGC TGGAGGAGCT GAGCGAGCGG CTGGAGGAGG CGGGAGGCGT CACCTCCACG 
CAGATAGAGC TCAACAAGAA GCGGGAGGCG GAGTTCCTGA AGCTGCGCAG GGACCTGGAG 
GAGGCCACAC TGCAGCACGA AGCCATGGTG GCCGCGCTGA GGAAGAAGCA TGCGGATAGT 
GTGGCCGAGC TTGGGGAGCA GATTGACAAC CTGCAGCGGG TCAAGCAGAA GCTGGAGAAG 
GAGAAGAGCG AGTTCAAGCT GGAGATCGAT GACCTCTCCA GCAGCATGGA GAGTGTGTCG 
AAATCTAAGG CAAATCTGGA AAAAATCTGC CGAACCCTGG AGGATCAGTT AAGTGAGGCC 
AGGGGCAAGA ATGAGGAAAT TCAGAGGAGC CTGAGCGAGC TGACCACACA GAAGTCTCGT 
TTGCAGACCG AGGCTGGTGA GCTGAGTCGT CAGCTGGAAG AAAAAGAAAG CATAGTATCC 
CAACTTTCCA GGAGCAAGCA AGCCTTTACC CAGCAAACAG AAGAGCTCAA GAGGCAGCTG 
GAGGAAGAGA ACAAGGCCAA GAACGCCCTG GCGCACGCCC TGCAGTCCTC CCGCCACGAC 
TGTGACCTGC TGCGGGAACA GTATGAGGAG GAGCAGGAAG GCAAAGCTGA GCTGCAGAGG 
GCGCTGTCCA AGGCCAATAG TGAGGTTGCC CAGTGGAGAA CCAAATACGA GACGGACGCC 
ATCCAGCGCA CAGAAGAGCT GGAGGAGGCC AAGAAAAAAC TTGCTCAGCG CCTTCAAGAT 
TCCGAGGAAC AGGTTGAGGC AGTGAATGCT AAATGTGCTT CACTGGAGAA GACCAAGCAG 
AGGCTGCAAG GAGAGGTGGA GGATCTGATG GTTGATGTTG AAAGAGCCAA TTCCTTGGCC 
GCCGCTCTGG ACAAGAAGCA GAGGAACTTT GACAAGGTGT TGGCAGAGTG GAAGACAAAG 
TGTGAGGAGA GCCAAGCAGA GCTGGAGGCA TCCCTGAAGG AGTCCCGCTC CTTGAGCACT 
GAGCTCTTCA AACTGAAAAA TGCCTACGAG GAAGCCTTAG ATCAACTTGA AACTGTGAAA 
CGGGAAAATA AGAACTTAGA GCAGGAGATA GCAGATCTCA CAGAACAAAT TGCTGAAAAT 
GGCAAAACCA TCCATGAACT GGAGAAATCA AGAAAGCAGA TTGAGCTGGA AAAGGCTGAT 
ATCCAGCTGG CTCTCGAGGA AGCAGAGGCT GCTCTTGAGC ATGAAGAAGC CAAGATCCTC 
CGAATCCAGC TTGAATTGAC ACAAGTGAAA TCAGAAATTG ATAGAAAGAT CGCCGAGAAG 
GATGAAGAGA TCGAGCAGCT GAAGAGGAAC TACCAGAGAA CAGTGGAAAC CATGCAGAGC 
GCCCTGGACG CCGAGGTGCG GAGCAGGAAT GAAGCCATCC GGCTCAAGAA GAAGATGGAG 
GGGGACCTGA ATGAAATCGA GATCCAGCTG AGCCACGCCA ACCGCCAGGC GGCGGAGACC 
CTCAAACACC TCAGGAGTGT CCAGGGACAG CTGAAGGATA CGCAGCTCCA CCTGGATGAT 
GCCCTCCGGG GCCAGGAGGA CCTGAAGGAG CAGCTGGCGA TTGTGGAGCG CAGAGCCAAC 
CTGCTGCAGG CCGAGGTGGA GGAGCTGCGG GCTACTCTGG AGCAGACGGA GAGGGCCCGG 
AAACTGGCGG AACAGGAGCT CCTGGACTCC AACGAGAGGG TGCAGCTGCT GCATACCCAG 
AACACCAGCC TCATCCACAC CAAGAAGAAG CTGGAGACAG ACCTCATGCA GCTCCAGAGT 
GAGGTAGAAG ATGCCAGCAG GGATGCAAGG AACGCTGAGG AGAAGGCCAA GAAGGCCATC 
ACGGACGCTG CCATGATGGC GGAGGAGCTG AAGAAGGAGC AGGACACCAG CGCCCACCTT 
GAGCGGATGA AGAAGAACCT GGAACAGACG GTGAAGGACC TGCAGCATCG TCTAGATGAG 
GCCGAGCAGC TGGCGCTGAA GGGCGGGAAG AAGCAGATCC AGAAACTGGA GACCAGGATC 
CGAGAGCTGG AGTTTGAACT TGAGGGAGAG CAGAAGAAGA ACACAGAGTC TGTTAAGGGC 
CTGAGGAAGT ATGAGCGGAG GGTCAAGGAG CTGACGTACC AGAGTGAAGA GGACAGGAAG 
AATGTGCTGA GATTGCAGGA TCTGGTGGAT AAACTGCAAG TGAAAGTCAA GTCCTACAAG 
AGGCAGGCGG AGGAGGCTGA TGAACAAGCC AATGCTCATC TCACCAAATT CCGAAAGGCT 
CAGCATGAGC TGGAGGAGGC CGAGGAACGT GCGGATATCG CAGAATCTCA AGTCAACAAG 
CTCCGCGCTA AGACTCGAGA CTTCACCTCC AGCAGGATGG TGGTCCACGA GAGTGAAGAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A novel pathogenic MYH3 mutation in a child with Sheldon-Hall syndrome and vertebral fusions.
American journal of medical genetics. Part A(2018 Jan)
Scala M,Accogli A,De Grandis E,Allegri A,Bagowski CP,Shoukier M,Maghnie M,Capra V

A postnatal role for embryonic myosin revealed by MYH3 mutations that alter TGF? signaling and cause autosomal dominant spondylocarpotarsal synostosis.
Scientific reports741803(2017 Feb)
Zieba J,Zhang W,Chong JX,Forlenza KN,Martin JH,Heard K,Grange DK,Butler MG,Kleefstra T,Lachman RS,Nickerson D,Regnier M,Cohn DH,Bamshad M,Krakow D

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MYH3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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