MYH2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MYH2
Entrez Gene ID	4620
Full Name	myosin heavy chain 2
Synonyms	IBM3,MYH2A,MYHSA2,MYHas8,MYPOP,MyHC-2A,MyHC-IIa
General protein information	Preferred Names myosin heavy chain 2 Names myosin-2 fast 2a myosin heavy chain inclusion body myopathy 3, autosomal dominant myosin heavy chain 2a myosin heavy chain IIa myosin heavy chain, skeletal muscle, adult 2 myosin, heavy chain 2, skeletal muscle, adult myosin, heavy polypeptide 2, skeletal muscle, adult type IIA myosin heavy chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells. Muscle myosins are heterohexamers composed of 2 myosin heavy chains and 2 pairs of nonidentical myosin light chains. This gene encodes a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction. This gene is found in a cluster of myosin heavy chain genes on chromosome 17. A mutation in this gene results in inclusion body myopathy-3. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Sep 2009].
Disorder MIM:	160740
Disorder Html:	Proximal myopathy and ophthalmoplegia, 605637 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_017534.5	NP_060004.3	myosin-2
NM_017534.6	NP_060004.3	myosin-2
NM_001100112.1	NP_001093582.1	myosin-2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MYH1C	NP_001107181.1
Mus musculus (house mouse)	Myh2	NP_001034634.2
Gallus gallus (chicken)	MYH1D	XP_415578.4
Gallus gallus (chicken)	MYH1E	NP_001013415.1
Bos taurus (cattle)	MYH2	NP_001159699.1
	MYH2	XP_002800336.1
Canis lupus familiaris (dog)	MYH2	NP_001070263.1
Gallus gallus (chicken)	MYH1A	NP_001013414.1
Gallus gallus (chicken)	MYH1F	XP_001231409.1
Homo sapiens (human)	MYH2	NP_001093582.1
Rattus norvegicus (Norway rat)	Myh2	NP_001128629.1
Gallus gallus (chicken)	MYH1B	NP_989559.1
Gallus gallus (chicken)	MYH1G	XP_003642358.1

Gene Ontology
Bibliography

Related articles in PubMed

Congenital myopathy due to myosin heavy chain 2 mutation presenting as chronic aspiration pneumonia in infancy.
Tsabari R, Daum H, Kerem E, Fellig Y, Dor T
Neuromuscular disorders : NMD27(10)947-950(2017 Oct)

Myosin IIA Heavy Chain Phosphorylation Mediates Adhesion Maturation and Protrusion in Three Dimensions.
Rai V, Thomas DG, Beach JR, Egelhoff TT
The Journal of biological chemistry292(8)3099-3111(2017 02)

Confinement Sensing and Signal Optimization via Piezo1/PKA and Myosin II Pathways.
Hung WC, Yang JR, Yankaskas CL, Wong BS, Wu PH, Pardo-Pastor C, Serra SA, Chiang MJ, Gu Z, Wirtz D, Valverde MA, Yang JT, Zhang J, Konstantopoulos K
Cell reports15(7)1430-1441(2016 05)

Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.
Qiu X, Chen D, Li M, Gao Y, Liu F, Zheng H, Chen S
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(10)2915-23(2015 Oct)

Mutations and sequence variation in the human myosin heavy chain IIa gene (MYH2).
Tajsharghi H, Darin N, Rekabdar E, Kyllerman M, Wahlstr?m J, Martinsson T, Oldfors A
European journal of human genetics : EJHG13(5)617-22(2005 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

C-terminal Myosin IIA Heavy Chain phosphorylation sites are critical for recruitment of Myosin IIA to lamellar protrusions and for marginal paxillin phosphorylation during active cell spreading.
Title: Myosin IIA Heavy Chain Phosphorylation Mediates Adhesion Maturation and Protrusion in Three Dimensions.

differential regulation of PKA and cell stiffness in unconfined versus confined cells is abrogated by dual, but not individual, inhibition of Piezo1 and myosin II.
Title: Confinement Sensing and Signal Optimization via Piezo1/PKA and Myosin II Pathways.

Transcriptional levels of MHC-I, MHC-IIa, and MHC-IIb in denervation groups were significantly down-regulated compared with controls
Title: Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.

Myosin isoforms impact single-fiber force generation and may lead to alterations in whole skeletal muscle performance.
Title: Molecular determinants of force production in human skeletal muscle fibers: effects of myosin isoform expression and cross-sectional area.

We have found that a greater MyH2 content in the vastus lateralis is accompanied by a higher oxygen cost of cycling during exercise performed below the lactate threshold.
Title: Myosin heavy chain composition in the vastus lateralis muscle in relation to oxygen uptake and heart rate during cycling in humans.

The following MYH2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MYH2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06054	NM_001100112.1 Latest version!	Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu06054	NM_017534.6 Latest version!	Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
View Old Accession Versions >>
OHu06054	NM_017534.5	Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MYH2 ( NM_017534.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060004.3 Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06054
Clone ID Related Accession (Same CDS sequence)	NM_017534.5 , NM_017534.6 , NM_001100112.1
Accession Version	NM_017534.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	myosin-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC379233.1, BC126409.1 and BC093082.1. This sequence is a reference standard in the RefSeqGene project. On Jul 25, 2007 this sequence version replaced NM_017534.4. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Both variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX510904.2, BC126409.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCAG ACTCAGAATT GGCTGTTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAAACATC TGTCTTTGTG 
GCGGAGCCCA AAGAATCCTT TGTCAAAGGG ACCATCCAGA GCAGAGAAGG AGGAAAAGTG 
ACGGTGAAGA CTGAGGGAGG AGCGACTCTG ACAGTGAAGG ATGATCAGGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAT ATGGCCATGA TGACTCATCT GCATGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAACGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGT 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCTG TGTATAAGCC CGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGTG AGAAGAAGAA GGAAGAAATT ACTTCTGGCA AAATACAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGAATCC ACTTTGGCAC TACTGGAAAA 
CTGGCATCTG CTGATATTGA AACATATCTG CTAGAGAAGT CTAGAGTTGT TTTCCAGCTT 
AAGGCTGAGA GAAGTTATCA TATTTTTTAC CAGATTACAT CGAATAAGAA ACCAGAACTT 
ATTGAAATGC TTCTGATTAC CACGAACCCA TATGATTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CCAGCATCGA TGATCAGGAA GAACTGATGG CCACAGATAG TGCTATTGAT 
ATTTTGGGCT TTACTAATGA AGAAAAGGTC TCCATTTACA AGCTCACGGG GGCTGTGATG 
CATTATGGGA ACCTAAAATT TAAGCAAAAG CAGCGTGAGG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CTGACAAGGC GGCCTACCTC CAGAGTCTGA ACTCTGCAGA TCTGCTCAAA 
GCTCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTA 
GAACAGGTGT CCAACGCAGT AGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG TTTTGAGATT TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TCGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCCGA GGCCCACTTC 
GCTCTGATTC ACTATGCTGG TGTTGTGGAC TACAACATTA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTTGGA CTGTACCAGA AGTCTGCAAT GAAAACTCTA 
GCTCAGCTCT TCTCTGGGGC TCAAACTGCT GAAGGAGAGG GAGCTGGTGG AGGGGCCAAG 
AAAGGTGGTA AGAAGAAGGG CTCTTCTTTC CAGACAGTGT CTGCCCTTTT CAGAGAGAAT 
TTGAACAAGC TGATGACCAA CCTCAGGAGT ACCCATCCTC ACTTTGTGAG GTGTATCATC 
CCCAATGAGA CAAAAACTCC TGGTGCCATG GAGCATGAGC TTGTCCTCCA CCAGCTGAGG 
TGTAACGGTG TGCTGGAAGG CATCCGCATC TGTAGGAAAG GATTTCCAAG CAGAATCCTT 
TATGCAGACT TCAAACAGAG ATACAAGGTA TTAAATGCAA GTGCAATCCC TGAAGGGCAA 
TTCATTGATA GCAAGAAGGC CTCTGAGAAG CTCCTTGCAT CCATCGACAT TGACCACACC 
CAGTATAAAT TTGGGCACAC CAAGGTCTTT TTCAAAGCTG GTCTTCTGGG GCTCCTAGAG 
GAGATGCGAG ATGACAAGCT GGCCCAGCTG ATTACCCGAA CCCAGGCCAG GTGCAGAGGG 
TTCTTGGCAA GAGTGGAGTA CCAGAGGATG GTGGAGAGAA GGGAGGCCAT CTTCTGTATC 
CAGTACAATA TCAGATCCTT CATGAATGTC AAGCACTGGC CCTGGATGAA ACTCTTCTTC 
AAGATCAAGC CTCTGTTGAA GAGTGCAGAA ACTGAGAAGG AGATGGCCAC CATGAAGGAA 
GAATTTCAGA AAATTAAAGA CGAACTTGCC AAGTCAGAGG CAAAAAGGAA GGAACTGGAA 
GAAAAGATGG TGACGCTGTT GAAAGAAAAA AATGACTTGC AGCTCCAAGT TCAGGCTGAA 
GCCGAAGGCT TGGCTGATGC AGAGGAAAGG TGTGACCAGC TAATCAAAAC CAAAATCCAG 
CTAGAAGCCA AAATCAAAGA GGTGACTGAG AGAGCTGAGG ATGAGGAAGA GATCAATGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAACTCAA GAAAGACATT 
GATGACCTTG AGCTGACACT GGCCAAGGTT GAGAAGGAGA AACATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAAGA GATGGCAGGT CTGGATGAAA CCATTGCTAA GCTGACCAAG 
GAGAAGAAGG CTCTCCAGGA GGCCCACCAG CAGACCCTGG ATGACCTGCA GGCAGAGGAG 
GACAAAGTCA ACACCCTGAC CAAAGCTAAA ATCAAACTTG AACAACAAGT GGATGATCTT 
GAAGGGTCCT TGGAGCAAGA AAAGAAACTT CGCATGGACC TAGAAAGGGC TAAGAGGAAA 
CTTGAGGGTG ACTTGAAGTT GGCCCAAGAA TCCATAATGG ACATTGAAAA TGAGAAACAG 
CAACTTGATG AAAAGCTCAA AAAGAAAGAG TTTGAAATCA GCAATCTGCA AAGCAAGATT 
GAAGATGAAC AGGCACTTGG CATTCAATTG CAGAAGAAAA TTAAAGAATT GCAAGCCCGC 
ATTGAGGAGC TGGAGGAGGA AATCGAGGCA GAGCGGGCCT CCCGGGCCAA AGCAGAGAAG 
CAGCGCTCTG ACCTCTCCCG GGAGCTGGAG GAGATCAGCG AGAGGCTGGA AGAAGCCGGT 
GGGGCCACTT CAGCCCAGAT TGAGATGAAC AAGAAGCGGG AGGCTGAGTT CCAGAAAATG 
CGCAGGGACC TGGAGGAGGC CACCCTACAG CATGAAGCCA CAGCGGCCAC CCTGAGGAAG 
AAGCATGCAG ATAGTGTGGC CGAGCTTGGG GAGCAGATTG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGATG AAGATGGAGA TTGATGACCT TGCTAGTAAT 
GTAGAAACGG TCTCCAAAGC CAAGGGAAAC CTAGAGAAAA TGTGCCGGAC TCTAGAGGAC 
CAACTGAGTG AACTGAAATC AAAGGAAGAG GAGCAGCAGC GGCTGATCAA TGACCTGACT 
GCGCAGAGGG GGCGCCTGCA GACTGAATCT GGTGAGTTTT CACGCCAGCT TGATGAAAAG 
GAAGCTCTGG TGTCTCAGTT ATCAAGAGGC AAACAAGCCT TTACTCAACA GATTGAAGAA 
TTAAAGAGGC AACTTGAAGA GGAGATAAAA GCCAAGAACG CCCTGGCGCA TGCCCTGCAG 
TCTTCCCGCC ACGACTGTGA CCTGCTGCGG GAACAGTATG AGGAGGAGCA GGAATCCAAG 
GCCGAGCTGC AGAGAGCACT GTCCAAGGCC AACACCGAGG TTGCCCAATG GAGGACCAAA 
TACGAGACGG ACGCCATCCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCC 
CAGCGGCTGC AGGCAGCTGA GGAACATGTA GAAGCTGTGA ACGCCAAATG TGCTTCCCTC 
GAAAAGACGA AGCAGCGGCT GCAGAATGAG GTCGAGGACC TCATGCTTGA TGTGGAGAGG 
ACAAATGCCG CCTGTGCCGC CCTTGACAAA AAGCAAAGGA ACTTCGATAA GATCCTGGCA 
GAATGGAAAC AGAAATGTGA GGAAACGCAT GCTGAGCTTG AGGCCTCCCA GAAGGAGGCC 
CGTTCCCTTG GCACTGAGCT GTTCAAGATA AAGAATGCCT ATGAGGAATC TTTGGATCAG 
CTAGAAACCC TGAAGCGAGA GAACAAAAAC TTACAGCAGG AGATTTCTGA CCTCACGGAA 
CAGATTGCAG AAGGAGGGAA ACGTATCCAT GAACTGGAGA AAATAAAGAA ACAAGTGGAA 
CAAGAAAAGT GTGAACTTCA GGCTGCTTTA GAAGAAGCAG AGGCATCTCT TGAACATGAA 
GAGGGAAAGA TCCTGCGCAT CCAGCTTGAG TTGAACCAAG TCAAGTCTGA GGTTGATAGG 
AAAATTGCTG AAAAAGATGA GGAAATTGAC CAGCTGAAGA GAAACCACAT TAGAATCGTG 
GAGTCCATGC AGAGCACGCT GGATGCTGAG ATCAGGAGTA GGAATGATGC CATTAGGCTC 
AAGAAGAAGA TGGAGGGAGA CCTCAATGAA ATGGAAATCC AGCTGAACCA TGCCAACCGC 
ATGGCTGCTG AGGCCCTGAG GAACTACAGG AACACCCAAG GCATCCTCAA GGATACCCAG 
ATCCACCTGG ATGATGCTCT CCGGAGCCAG GAGGACCTGA AGGAACAGCT GGCCATGGTG 
GAGCGCAGAG CCAACCTGCT GCAGGCTGAG ATCGAGGAGC TGCGGGCCAC TCTGGAACAG 
ACAGAGAGGA GCAGAAAAAT CGCAGAACAG GAGCTCCTGG ATGCCAGTGA GCGTGTTCAG 
CTACTGCACA CCCAGAACAC CAGCCTGATC AACACCAAGA AGAAGCTGGA GACAGATATT 
TCCCAAATGC AAGGAGAGAT GGAGGACATT CTCCAGGAAG CCCGCAATGC AGAAGAAAAG 
GCCAAGAAGG CCATCACTGA TGCCGCCATG ATGGCTGAGG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAGCG GATGAAGAAG AACATGGAGC AGACCGTGAA GGATCTGCAG 
CTCCGTCTGG ATGAGGCTGA GCAGCTGGCC CTGAAGGGTG GGAAGAAGCA GATCCAGAAA 
CTGGAGGCCA GGGTACGGGA GCTGGAAGGA GAGGTTGAGA GTGAGCAAAA GCGTAATGCT 
GAGGCTGTCA AAGGTCTGCG CAAACATGAG AGGCGAGTGA AGGAACTCAC TTACCAGACG 
GAAGAAGATA GAAAGAATAT TCTCAGGCTT CAAGATTTGG TAGATAAACT TCAGGCAAAA 
GTGAAATCTT ATAAGAGACA AGCTGAGGAG GCTGAGGAAC AATCCAACAC CAATCTAGCT 
AAATTCCGCA AGCTCCAGCA TGAGCTGGAG GAGGCCGAGG AACGGGCTGA CATTGCTGAG 
TCCCAGGTGA ACAAACTGCG GGTGAAGAGC CGGGAGGTTC ACACAAAAGT CATAAGTGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060004.3
CDS	129..5954
Translation	MSSDSELAVFGEAAPFLRKSERERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSREGGKVTVKTEGGATLTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEITSGKIQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVVFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTVEQVSNAVGALAKAVYEKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKNKDPLNETVVGLYQKSAMKTLAQLFSGAQTAEGEGAGGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQRMVERREAIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKIKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEISNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQLSELKSKEEEQQRLINDLTAQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANTEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKEARSLGTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQMQGEMEDILQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	NM_017534.5
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017534.5

ORF Insert Sequence:

ATGAGTTCAG ACTCAGAATT GGCTGTTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAAACATC TGTCTTTGTG 
GCGGAGCCCA AAGAATCCTT TGTCAAAGGG ACCATCCAGA GCAGAGAAGG AGGAAAAGTG 
ACGGTGAAGA CTGAGGGAGG AGCGACTCTG ACAGTGAAGG ATGATCAGGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAT ATGGCCATGA TGACTCATCT GCATGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAACGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGT 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCTG TGTATAAGCC CGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGTG AGAAGAAGAA GGAAGAAATT ACTTCTGGCA AAATACAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGAATCC ACTTTGGCAC TACTGGAAAA 
CTGGCATCTG CTGATATTGA AACATATCTG CTAGAGAAGT CTAGAGTTGT TTTCCAGCTT 
AAGGCTGAGA GAAGTTATCA TATTTTTTAC CAGATTACAT CGAATAAGAA ACCAGAACTT 
ATTGAAATGC TTCTGATTAC CACGAACCCA TATGATTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CCAGCATCGA TGATCAGGAA GAACTGATGG CCACAGATAG TGCTATTGAT 
ATTTTGGGCT TTACTAATGA AGAAAAGGTC TCCATTTACA AGCTCACGGG GGCTGTGATG 
CATTATGGGA ACCTAAAATT TAAGCAAAAG CAGCGTGAGG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CTGACAAGGC GGCCTACCTC CAGAGTCTGA ACTCTGCAGA TCTGCTCAAA 
GCTCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTA 
GAACAGGTGT CCAACGCAGT AGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG TTTTGAGATT TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TCGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCCGA GGCCCACTTC 
GCTCTGATTC ACTATGCTGG TGTTGTGGAC TACAACATTA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTTGGA CTGTACCAGA AGTCTGCAAT GAAAACTCTA 
GCTCAGCTCT TCTCTGGGGC TCAAACTGCT GAAGGAGAGG GAGCTGGTGG AGGGGCCAAG 
AAAGGTGGTA AGAAGAAGGG CTCTTCTTTC CAGACAGTGT CTGCCCTTTT CAGAGAGAAT 
TTGAACAAGC TGATGACCAA CCTCAGGAGT ACCCATCCTC ACTTTGTGAG GTGTATCATC 
CCCAATGAGA CAAAAACTCC TGGTGCCATG GAGCATGAGC TTGTCCTCCA CCAGCTGAGG 
TGTAACGGTG TGCTGGAAGG CATCCGCATC TGTAGGAAAG GATTTCCAAG CAGAATCCTT 
TATGCAGACT TCAAACAGAG ATACAAGGTA TTAAATGCAA GTGCAATCCC TGAAGGGCAA 
TTCATTGATA GCAAGAAGGC CTCTGAGAAG CTCCTTGCAT CCATCGACAT TGACCACACC 
CAGTATAAAT TTGGGCACAC CAAGGTCTTT TTCAAAGCTG GTCTTCTGGG GCTCCTAGAG 
GAGATGCGAG ATGACAAGCT GGCCCAGCTG ATTACCCGAA CCCAGGCCAG GTGCAGAGGG 
TTCTTGGCAA GAGTGGAGTA CCAGAGGATG GTGGAGAGAA GGGAGGCCAT CTTCTGTATC 
CAGTACAATA TCAGATCCTT CATGAATGTC AAGCACTGGC CCTGGATGAA ACTCTTCTTC 
AAGATCAAGC CTCTGTTGAA GAGTGCAGAA ACTGAGAAGG AGATGGCCAC CATGAAGGAA 
GAATTTCAGA AAATTAAAGA CGAACTTGCC AAGTCAGAGG CAAAAAGGAA GGAACTGGAA 
GAAAAGATGG TGACGCTGTT GAAAGAAAAA AATGACTTGC AGCTCCAAGT TCAGGCTGAA 
GCCGAAGGCT TGGCTGATGC AGAGGAAAGG TGTGACCAGC TAATCAAAAC CAAAATCCAG 
CTAGAAGCCA AAATCAAAGA GGTGACTGAG AGAGCTGAGG ATGAGGAAGA GATCAATGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAACTCAA GAAAGACATT 
GATGACCTTG AGCTGACACT GGCCAAGGTT GAGAAGGAGA AACATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAAGA GATGGCAGGT CTGGATGAAA CCATTGCTAA GCTGACCAAG 
GAGAAGAAGG CTCTCCAGGA GGCCCACCAG CAGACCCTGG ATGACCTGCA GGCAGAGGAG 
GACAAAGTCA ACACCCTGAC CAAAGCTAAA ATCAAACTTG AACAACAAGT GGATGATCTT 
GAAGGGTCCT TGGAGCAAGA AAAGAAACTT CGCATGGACC TAGAAAGGGC TAAGAGGAAA 
CTTGAGGGTG ACTTGAAGTT GGCCCAAGAA TCCATAATGG ACATTGAAAA TGAGAAACAG 
CAACTTGATG AAAAGCTCAA AAAGAAAGAG TTTGAAATCA GCAATCTGCA AAGCAAGATT 
GAAGATGAAC AGGCACTTGG CATTCAATTG CAGAAGAAAA TTAAAGAATT GCAAGCCCGC 
ATTGAGGAGC TGGAGGAGGA AATCGAGGCA GAGCGGGCCT CCCGGGCCAA AGCAGAGAAG 
CAGCGCTCTG ACCTCTCCCG GGAGCTGGAG GAGATCAGCG AGAGGCTGGA AGAAGCCGGT 
GGGGCCACTT CAGCCCAGAT TGAGATGAAC AAGAAGCGGG AGGCTGAGTT CCAGAAAATG 
CGCAGGGACC TGGAGGAGGC CACCCTACAG CATGAAGCCA CAGCGGCCAC CCTGAGGAAG 
AAGCATGCAG ATAGTGTGGC CGAGCTTGGG GAGCAGATTG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGATG AAGATGGAGA TTGATGACCT TGCTAGTAAT 
GTAGAAACGG TCTCCAAAGC CAAGGGAAAC CTAGAGAAAA TGTGCCGGAC TCTAGAGGAC 
CAACTGAGTG AACTGAAATC AAAGGAAGAG GAGCAGCAGC GGCTGATCAA TGACCTGACT 
GCGCAGAGGG GGCGCCTGCA GACTGAATCT GGTGAGTTTT CACGCCAGCT TGATGAAAAG 
GAAGCTCTGG TGTCTCAGTT ATCAAGAGGC AAACAAGCCT TTACTCAACA GATTGAAGAA 
TTAAAGAGGC AACTTGAAGA GGAGATAAAA GCCAAGAACG CCCTGGCGCA TGCCCTGCAG 
TCTTCCCGCC ACGACTGTGA CCTGCTGCGG GAACAGTATG AGGAGGAGCA GGAATCCAAG 
GCCGAGCTGC AGAGAGCACT GTCCAAGGCC AACACCGAGG TTGCCCAATG GAGGACCAAA 
TACGAGACGG ACGCCATCCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCC 
CAGCGGCTGC AGGCAGCTGA GGAACATGTA GAAGCTGTGA ACGCCAAATG TGCTTCCCTC 
GAAAAGACGA AGCAGCGGCT GCAGAATGAG GTCGAGGACC TCATGCTTGA TGTGGAGAGG 
ACAAATGCCG CCTGTGCCGC CCTTGACAAA AAGCAAAGGA ACTTCGATAA GATCCTGGCA 
GAATGGAAAC AGAAATGTGA GGAAACGCAT GCTGAGCTTG AGGCCTCCCA GAAGGAGGCC 
CGTTCCCTTG GCACTGAGCT GTTCAAGATA AAGAATGCCT ATGAGGAATC TTTGGATCAG 
CTAGAAACCC TGAAGCGAGA GAACAAAAAC TTACAGCAGG AGATTTCTGA CCTCACGGAA 
CAGATTGCAG AAGGAGGGAA ACGTATCCAT GAACTGGAGA AAATAAAGAA ACAAGTGGAA 
CAAGAAAAGT GTGAACTTCA GGCTGCTTTA GAAGAAGCAG AGGCATCTCT TGAACATGAA 
GAGGGAAAGA TCCTGCGCAT CCAGCTTGAG TTGAACCAAG TCAAGTCTGA GGTTGATAGG 
AAAATTGCTG AAAAAGATGA GGAAATTGAC CAGCTGAAGA GAAACCACAT TAGAATCGTG 
GAGTCCATGC AGAGCACGCT GGATGCTGAG ATCAGGAGTA GGAATGATGC CATTAGGCTC 
AAGAAGAAGA TGGAGGGAGA CCTCAATGAA ATGGAAATCC AGCTGAACCA TGCCAACCGC 
ATGGCTGCTG AGGCCCTGAG GAACTACAGG AACACCCAAG GCATCCTCAA GGATACCCAG 
ATCCACCTGG ATGATGCTCT CCGGAGCCAG GAGGACCTGA AGGAACAGCT GGCCATGGTG 
GAGCGCAGAG CCAACCTGCT GCAGGCTGAG ATCGAGGAGC TGCGGGCCAC TCTGGAACAG 
ACAGAGAGGA GCAGAAAAAT CGCAGAACAG GAGCTCCTGG ATGCCAGTGA GCGTGTTCAG 
CTACTGCACA CCCAGAACAC CAGCCTGATC AACACCAAGA AGAAGCTGGA GACAGATATT 
TCCCAAATGC AAGGAGAGAT GGAGGACATT CTCCAGGAAG CCCGCAATGC AGAAGAAAAG 
GCCAAGAAGG CCATCACTGA TGCCGCCATG ATGGCTGAGG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAGCG GATGAAGAAG AACATGGAGC AGACCGTGAA GGATCTGCAG 
CTCCGTCTGG ATGAGGCTGA GCAGCTGGCC CTGAAGGGTG GGAAGAAGCA GATCCAGAAA 
CTGGAGGCCA GGGTACGGGA GCTGGAAGGA GAGGTTGAGA GTGAGCAAAA GCGTAATGCT 
GAGGCTGTCA AAGGTCTGCG CAAACATGAG AGGCGAGTGA AGGAACTCAC TTACCAGACG 
GAAGAAGATA GAAAGAATAT TCTCAGGCTT CAAGATTTGG TAGATAAACT TCAGGCAAAA 
GTGAAATCTT ATAAGAGACA AGCTGAGGAG GCTGAGGAAC AATCCAACAC CAATCTAGCT 
AAATTCCGCA AGCTCCAGCA TGAGCTGGAG GAGGCCGAGG AACGGGCTGA CATTGCTGAG 
TCCCAGGTGA ACAAACTGCG GGTGAAGAGC CGGGAGGTTC ACACAAAAGT CATAAGTGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH2 ( NM_017534.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060004.3 Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 1, mRNA.

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CloneID	OHu06054
Clone ID Related Accession (Same CDS sequence)	NM_017534.5 , NM_017534.6 , NM_001100112.1
Accession Version	NM_017534.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	myosin-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC379233.1, BC126409.1 and BC093082.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_017534.5. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Both variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX510904.2, BC126409.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000245503.10/ ENSP00000245503.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTTCAG ACTCAGAATT GGCTGTTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAAACATC TGTCTTTGTG 
GCGGAGCCCA AAGAATCCTT TGTCAAAGGG ACCATCCAGA GCAGAGAAGG AGGAAAAGTG 
ACGGTGAAGA CTGAGGGAGG AGCGACTCTG ACAGTGAAGG ATGATCAGGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAT ATGGCCATGA TGACTCATCT GCATGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAACGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGT 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCTG TGTATAAGCC CGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGTG AGAAGAAGAA GGAAGAAATT ACTTCTGGCA AAATACAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGAATCC ACTTTGGCAC TACTGGAAAA 
CTGGCATCTG CTGATATTGA AACATATCTG CTAGAGAAGT CTAGAGTTGT TTTCCAGCTT 
AAGGCTGAGA GAAGTTATCA TATTTTTTAC CAGATTACAT CGAATAAGAA ACCAGAACTT 
ATTGAAATGC TTCTGATTAC CACGAACCCA TATGATTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CCAGCATCGA TGATCAGGAA GAACTGATGG CCACAGATAG TGCTATTGAT 
ATTTTGGGCT TTACTAATGA AGAAAAGGTC TCCATTTACA AGCTCACGGG GGCTGTGATG 
CATTATGGGA ACCTAAAATT TAAGCAAAAG CAGCGTGAGG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CTGACAAGGC GGCCTACCTC CAGAGTCTGA ACTCTGCAGA TCTGCTCAAA 
GCTCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTA 
GAACAGGTGT CCAACGCAGT AGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG TTTTGAGATT TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TCGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCCGA GGCCCACTTC 
GCTCTGATTC ACTATGCTGG TGTTGTGGAC TACAACATTA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTTGGA CTGTACCAGA AGTCTGCAAT GAAAACTCTA 
GCTCAGCTCT TCTCTGGGGC TCAAACTGCT GAAGGAGAGG GAGCTGGTGG AGGGGCCAAG 
AAAGGTGGTA AGAAGAAGGG CTCTTCTTTC CAGACAGTGT CTGCCCTTTT CAGAGAGAAT 
TTGAACAAGC TGATGACCAA CCTCAGGAGT ACCCATCCTC ACTTTGTGAG GTGTATCATC 
CCCAATGAGA CAAAAACTCC TGGTGCCATG GAGCATGAGC TTGTCCTCCA CCAGCTGAGG 
TGTAACGGTG TGCTGGAAGG CATCCGCATC TGTAGGAAAG GATTTCCAAG CAGAATCCTT 
TATGCAGACT TCAAACAGAG ATACAAGGTA TTAAATGCAA GTGCAATCCC TGAAGGGCAA 
TTCATTGATA GCAAGAAGGC CTCTGAGAAG CTCCTTGCAT CCATCGACAT TGACCACACC 
CAGTATAAAT TTGGGCACAC CAAGGTCTTT TTCAAAGCTG GTCTTCTGGG GCTCCTAGAG 
GAGATGCGAG ATGACAAGCT GGCCCAGCTG ATTACCCGAA CCCAGGCCAG GTGCAGAGGG 
TTCTTGGCAA GAGTGGAGTA CCAGAGGATG GTGGAGAGAA GGGAGGCCAT CTTCTGTATC 
CAGTACAATA TCAGATCCTT CATGAATGTC AAGCACTGGC CCTGGATGAA ACTCTTCTTC 
AAGATCAAGC CTCTGTTGAA GAGTGCAGAA ACTGAGAAGG AGATGGCCAC CATGAAGGAA 
GAATTTCAGA AAATTAAAGA CGAACTTGCC AAGTCAGAGG CAAAAAGGAA GGAACTGGAA 
GAAAAGATGG TGACGCTGTT GAAAGAAAAA AATGACTTGC AGCTCCAAGT TCAGGCTGAA 
GCCGAAGGCT TGGCTGATGC AGAGGAAAGG TGTGACCAGC TAATCAAAAC CAAAATCCAG 
CTAGAAGCCA AAATCAAAGA GGTGACTGAG AGAGCTGAGG ATGAGGAAGA GATCAATGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAACTCAA GAAAGACATT 
GATGACCTTG AGCTGACACT GGCCAAGGTT GAGAAGGAGA AACATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAAGA GATGGCAGGT CTGGATGAAA CCATTGCTAA GCTGACCAAG 
GAGAAGAAGG CTCTCCAGGA GGCCCACCAG CAGACCCTGG ATGACCTGCA GGCAGAGGAG 
GACAAAGTCA ACACCCTGAC CAAAGCTAAA ATCAAACTTG AACAACAAGT GGATGATCTT 
GAAGGGTCCT TGGAGCAAGA AAAGAAACTT CGCATGGACC TAGAAAGGGC TAAGAGGAAA 
CTTGAGGGTG ACTTGAAGTT GGCCCAAGAA TCCATAATGG ACATTGAAAA TGAGAAACAG 
CAACTTGATG AAAAGCTCAA AAAGAAAGAG TTTGAAATCA GCAATCTGCA AAGCAAGATT 
GAAGATGAAC AGGCACTTGG CATTCAATTG CAGAAGAAAA TTAAAGAATT GCAAGCCCGC 
ATTGAGGAGC TGGAGGAGGA AATCGAGGCA GAGCGGGCCT CCCGGGCCAA AGCAGAGAAG 
CAGCGCTCTG ACCTCTCCCG GGAGCTGGAG GAGATCAGCG AGAGGCTGGA AGAAGCCGGT 
GGGGCCACTT CAGCCCAGAT TGAGATGAAC AAGAAGCGGG AGGCTGAGTT CCAGAAAATG 
CGCAGGGACC TGGAGGAGGC CACCCTACAG CATGAAGCCA CAGCGGCCAC CCTGAGGAAG 
AAGCATGCAG ATAGTGTGGC CGAGCTTGGG GAGCAGATTG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGATG AAGATGGAGA TTGATGACCT TGCTAGTAAT 
GTAGAAACGG TCTCCAAAGC CAAGGGAAAC CTAGAGAAAA TGTGCCGGAC TCTAGAGGAC 
CAACTGAGTG AACTGAAATC AAAGGAAGAG GAGCAGCAGC GGCTGATCAA TGACCTGACT 
GCGCAGAGGG GGCGCCTGCA GACTGAATCT GGTGAGTTTT CACGCCAGCT TGATGAAAAG 
GAAGCTCTGG TGTCTCAGTT ATCAAGAGGC AAACAAGCCT TTACTCAACA GATTGAAGAA 
TTAAAGAGGC AACTTGAAGA GGAGATAAAA GCCAAGAACG CCCTGGCGCA TGCCCTGCAG 
TCTTCCCGCC ACGACTGTGA CCTGCTGCGG GAACAGTATG AGGAGGAGCA GGAATCCAAG 
GCCGAGCTGC AGAGAGCACT GTCCAAGGCC AACACCGAGG TTGCCCAATG GAGGACCAAA 
TACGAGACGG ACGCCATCCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCC 
CAGCGGCTGC AGGCAGCTGA GGAACATGTA GAAGCTGTGA ACGCCAAATG TGCTTCCCTC 
GAAAAGACGA AGCAGCGGCT GCAGAATGAG GTCGAGGACC TCATGCTTGA TGTGGAGAGG 
ACAAATGCCG CCTGTGCCGC CCTTGACAAA AAGCAAAGGA ACTTCGATAA GATCCTGGCA 
GAATGGAAAC AGAAATGTGA GGAAACGCAT GCTGAGCTTG AGGCCTCCCA GAAGGAGGCC 
CGTTCCCTTG GCACTGAGCT GTTCAAGATA AAGAATGCCT ATGAGGAATC TTTGGATCAG 
CTAGAAACCC TGAAGCGAGA GAACAAAAAC TTACAGCAGG AGATTTCTGA CCTCACGGAA 
CAGATTGCAG AAGGAGGGAA ACGTATCCAT GAACTGGAGA AAATAAAGAA ACAAGTGGAA 
CAAGAAAAGT GTGAACTTCA GGCTGCTTTA GAAGAAGCAG AGGCATCTCT TGAACATGAA 
GAGGGAAAGA TCCTGCGCAT CCAGCTTGAG TTGAACCAAG TCAAGTCTGA GGTTGATAGG 
AAAATTGCTG AAAAAGATGA GGAAATTGAC CAGCTGAAGA GAAACCACAT TAGAATCGTG 
GAGTCCATGC AGAGCACGCT GGATGCTGAG ATCAGGAGTA GGAATGATGC CATTAGGCTC 
AAGAAGAAGA TGGAGGGAGA CCTCAATGAA ATGGAAATCC AGCTGAACCA TGCCAACCGC 
ATGGCTGCTG AGGCCCTGAG GAACTACAGG AACACCCAAG GCATCCTCAA GGATACCCAG 
ATCCACCTGG ATGATGCTCT CCGGAGCCAG GAGGACCTGA AGGAACAGCT GGCCATGGTG 
GAGCGCAGAG CCAACCTGCT GCAGGCTGAG ATCGAGGAGC TGCGGGCCAC TCTGGAACAG 
ACAGAGAGGA GCAGAAAAAT CGCAGAACAG GAGCTCCTGG ATGCCAGTGA GCGTGTTCAG 
CTACTGCACA CCCAGAACAC CAGCCTGATC AACACCAAGA AGAAGCTGGA GACAGATATT 
TCCCAAATGC AAGGAGAGAT GGAGGACATT CTCCAGGAAG CCCGCAATGC AGAAGAAAAG 
GCCAAGAAGG CCATCACTGA TGCCGCCATG ATGGCTGAGG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAGCG GATGAAGAAG AACATGGAGC AGACCGTGAA GGATCTGCAG 
CTCCGTCTGG ATGAGGCTGA GCAGCTGGCC CTGAAGGGTG GGAAGAAGCA GATCCAGAAA 
CTGGAGGCCA GGGTACGGGA GCTGGAAGGA GAGGTTGAGA GTGAGCAAAA GCGTAATGCT 
GAGGCTGTCA AAGGTCTGCG CAAACATGAG AGGCGAGTGA AGGAACTCAC TTACCAGACG 
GAAGAAGATA GAAAGAATAT TCTCAGGCTT CAAGATTTGG TAGATAAACT TCAGGCAAAA 
GTGAAATCTT ATAAGAGACA AGCTGAGGAG GCTGAGGAAC AATCCAACAC CAATCTAGCT 
AAATTCCGCA AGCTCCAGCA TGAGCTGGAG GAGGCCGAGG AACGGGCTGA CATTGCTGAG 
TCCCAGGTGA ACAAACTGCG GGTGAAGAGC CGGGAGGTTC ACACAAAAGT CATAAGTGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060004.3
CDS	87..5912
Translation	MSSDSELAVFGEAAPFLRKSERERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSREGGKVTVKTEGGATLTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEITSGKIQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVVFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTVEQVSNAVGALAKAVYEKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKNKDPLNETVVGLYQKSAMKTLAQLFSGAQTAEGEGAGGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQRMVERREAIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKIKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEISNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQLSELKSKEEEQQRLINDLTAQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANTEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKEARSLGTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQMQGEMEDILQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	NM_017534.6
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017534.6

ORF Insert Sequence:

ATGAGTTCAG ACTCAGAATT GGCTGTTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAAACATC TGTCTTTGTG 
GCGGAGCCCA AAGAATCCTT TGTCAAAGGG ACCATCCAGA GCAGAGAAGG AGGAAAAGTG 
ACGGTGAAGA CTGAGGGAGG AGCGACTCTG ACAGTGAAGG ATGATCAGGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAT ATGGCCATGA TGACTCATCT GCATGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAACGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGT 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCTG TGTATAAGCC CGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGTG AGAAGAAGAA GGAAGAAATT ACTTCTGGCA AAATACAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGAATCC ACTTTGGCAC TACTGGAAAA 
CTGGCATCTG CTGATATTGA AACATATCTG CTAGAGAAGT CTAGAGTTGT TTTCCAGCTT 
AAGGCTGAGA GAAGTTATCA TATTTTTTAC CAGATTACAT CGAATAAGAA ACCAGAACTT 
ATTGAAATGC TTCTGATTAC CACGAACCCA TATGATTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CCAGCATCGA TGATCAGGAA GAACTGATGG CCACAGATAG TGCTATTGAT 
ATTTTGGGCT TTACTAATGA AGAAAAGGTC TCCATTTACA AGCTCACGGG GGCTGTGATG 
CATTATGGGA ACCTAAAATT TAAGCAAAAG CAGCGTGAGG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CTGACAAGGC GGCCTACCTC CAGAGTCTGA ACTCTGCAGA TCTGCTCAAA 
GCTCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTA 
GAACAGGTGT CCAACGCAGT AGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG TTTTGAGATT TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TCGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCCGA GGCCCACTTC 
GCTCTGATTC ACTATGCTGG TGTTGTGGAC TACAACATTA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTTGGA CTGTACCAGA AGTCTGCAAT GAAAACTCTA 
GCTCAGCTCT TCTCTGGGGC TCAAACTGCT GAAGGAGAGG GAGCTGGTGG AGGGGCCAAG 
AAAGGTGGTA AGAAGAAGGG CTCTTCTTTC CAGACAGTGT CTGCCCTTTT CAGAGAGAAT 
TTGAACAAGC TGATGACCAA CCTCAGGAGT ACCCATCCTC ACTTTGTGAG GTGTATCATC 
CCCAATGAGA CAAAAACTCC TGGTGCCATG GAGCATGAGC TTGTCCTCCA CCAGCTGAGG 
TGTAACGGTG TGCTGGAAGG CATCCGCATC TGTAGGAAAG GATTTCCAAG CAGAATCCTT 
TATGCAGACT TCAAACAGAG ATACAAGGTA TTAAATGCAA GTGCAATCCC TGAAGGGCAA 
TTCATTGATA GCAAGAAGGC CTCTGAGAAG CTCCTTGCAT CCATCGACAT TGACCACACC 
CAGTATAAAT TTGGGCACAC CAAGGTCTTT TTCAAAGCTG GTCTTCTGGG GCTCCTAGAG 
GAGATGCGAG ATGACAAGCT GGCCCAGCTG ATTACCCGAA CCCAGGCCAG GTGCAGAGGG 
TTCTTGGCAA GAGTGGAGTA CCAGAGGATG GTGGAGAGAA GGGAGGCCAT CTTCTGTATC 
CAGTACAATA TCAGATCCTT CATGAATGTC AAGCACTGGC CCTGGATGAA ACTCTTCTTC 
AAGATCAAGC CTCTGTTGAA GAGTGCAGAA ACTGAGAAGG AGATGGCCAC CATGAAGGAA 
GAATTTCAGA AAATTAAAGA CGAACTTGCC AAGTCAGAGG CAAAAAGGAA GGAACTGGAA 
GAAAAGATGG TGACGCTGTT GAAAGAAAAA AATGACTTGC AGCTCCAAGT TCAGGCTGAA 
GCCGAAGGCT TGGCTGATGC AGAGGAAAGG TGTGACCAGC TAATCAAAAC CAAAATCCAG 
CTAGAAGCCA AAATCAAAGA GGTGACTGAG AGAGCTGAGG ATGAGGAAGA GATCAATGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAACTCAA GAAAGACATT 
GATGACCTTG AGCTGACACT GGCCAAGGTT GAGAAGGAGA AACATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAAGA GATGGCAGGT CTGGATGAAA CCATTGCTAA GCTGACCAAG 
GAGAAGAAGG CTCTCCAGGA GGCCCACCAG CAGACCCTGG ATGACCTGCA GGCAGAGGAG 
GACAAAGTCA ACACCCTGAC CAAAGCTAAA ATCAAACTTG AACAACAAGT GGATGATCTT 
GAAGGGTCCT TGGAGCAAGA AAAGAAACTT CGCATGGACC TAGAAAGGGC TAAGAGGAAA 
CTTGAGGGTG ACTTGAAGTT GGCCCAAGAA TCCATAATGG ACATTGAAAA TGAGAAACAG 
CAACTTGATG AAAAGCTCAA AAAGAAAGAG TTTGAAATCA GCAATCTGCA AAGCAAGATT 
GAAGATGAAC AGGCACTTGG CATTCAATTG CAGAAGAAAA TTAAAGAATT GCAAGCCCGC 
ATTGAGGAGC TGGAGGAGGA AATCGAGGCA GAGCGGGCCT CCCGGGCCAA AGCAGAGAAG 
CAGCGCTCTG ACCTCTCCCG GGAGCTGGAG GAGATCAGCG AGAGGCTGGA AGAAGCCGGT 
GGGGCCACTT CAGCCCAGAT TGAGATGAAC AAGAAGCGGG AGGCTGAGTT CCAGAAAATG 
CGCAGGGACC TGGAGGAGGC CACCCTACAG CATGAAGCCA CAGCGGCCAC CCTGAGGAAG 
AAGCATGCAG ATAGTGTGGC CGAGCTTGGG GAGCAGATTG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGATG AAGATGGAGA TTGATGACCT TGCTAGTAAT 
GTAGAAACGG TCTCCAAAGC CAAGGGAAAC CTAGAGAAAA TGTGCCGGAC TCTAGAGGAC 
CAACTGAGTG AACTGAAATC AAAGGAAGAG GAGCAGCAGC GGCTGATCAA TGACCTGACT 
GCGCAGAGGG GGCGCCTGCA GACTGAATCT GGTGAGTTTT CACGCCAGCT TGATGAAAAG 
GAAGCTCTGG TGTCTCAGTT ATCAAGAGGC AAACAAGCCT TTACTCAACA GATTGAAGAA 
TTAAAGAGGC AACTTGAAGA GGAGATAAAA GCCAAGAACG CCCTGGCGCA TGCCCTGCAG 
TCTTCCCGCC ACGACTGTGA CCTGCTGCGG GAACAGTATG AGGAGGAGCA GGAATCCAAG 
GCCGAGCTGC AGAGAGCACT GTCCAAGGCC AACACCGAGG TTGCCCAATG GAGGACCAAA 
TACGAGACGG ACGCCATCCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCC 
CAGCGGCTGC AGGCAGCTGA GGAACATGTA GAAGCTGTGA ACGCCAAATG TGCTTCCCTC 
GAAAAGACGA AGCAGCGGCT GCAGAATGAG GTCGAGGACC TCATGCTTGA TGTGGAGAGG 
ACAAATGCCG CCTGTGCCGC CCTTGACAAA AAGCAAAGGA ACTTCGATAA GATCCTGGCA 
GAATGGAAAC AGAAATGTGA GGAAACGCAT GCTGAGCTTG AGGCCTCCCA GAAGGAGGCC 
CGTTCCCTTG GCACTGAGCT GTTCAAGATA AAGAATGCCT ATGAGGAATC TTTGGATCAG 
CTAGAAACCC TGAAGCGAGA GAACAAAAAC TTACAGCAGG AGATTTCTGA CCTCACGGAA 
CAGATTGCAG AAGGAGGGAA ACGTATCCAT GAACTGGAGA AAATAAAGAA ACAAGTGGAA 
CAAGAAAAGT GTGAACTTCA GGCTGCTTTA GAAGAAGCAG AGGCATCTCT TGAACATGAA 
GAGGGAAAGA TCCTGCGCAT CCAGCTTGAG TTGAACCAAG TCAAGTCTGA GGTTGATAGG 
AAAATTGCTG AAAAAGATGA GGAAATTGAC CAGCTGAAGA GAAACCACAT TAGAATCGTG 
GAGTCCATGC AGAGCACGCT GGATGCTGAG ATCAGGAGTA GGAATGATGC CATTAGGCTC 
AAGAAGAAGA TGGAGGGAGA CCTCAATGAA ATGGAAATCC AGCTGAACCA TGCCAACCGC 
ATGGCTGCTG AGGCCCTGAG GAACTACAGG AACACCCAAG GCATCCTCAA GGATACCCAG 
ATCCACCTGG ATGATGCTCT CCGGAGCCAG GAGGACCTGA AGGAACAGCT GGCCATGGTG 
GAGCGCAGAG CCAACCTGCT GCAGGCTGAG ATCGAGGAGC TGCGGGCCAC TCTGGAACAG 
ACAGAGAGGA GCAGAAAAAT CGCAGAACAG GAGCTCCTGG ATGCCAGTGA GCGTGTTCAG 
CTACTGCACA CCCAGAACAC CAGCCTGATC AACACCAAGA AGAAGCTGGA GACAGATATT 
TCCCAAATGC AAGGAGAGAT GGAGGACATT CTCCAGGAAG CCCGCAATGC AGAAGAAAAG 
GCCAAGAAGG CCATCACTGA TGCCGCCATG ATGGCTGAGG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAGCG GATGAAGAAG AACATGGAGC AGACCGTGAA GGATCTGCAG 
CTCCGTCTGG ATGAGGCTGA GCAGCTGGCC CTGAAGGGTG GGAAGAAGCA GATCCAGAAA 
CTGGAGGCCA GGGTACGGGA GCTGGAAGGA GAGGTTGAGA GTGAGCAAAA GCGTAATGCT 
GAGGCTGTCA AAGGTCTGCG CAAACATGAG AGGCGAGTGA AGGAACTCAC TTACCAGACG 
GAAGAAGATA GAAAGAATAT TCTCAGGCTT CAAGATTTGG TAGATAAACT TCAGGCAAAA 
GTGAAATCTT ATAAGAGACA AGCTGAGGAG GCTGAGGAAC AATCCAACAC CAATCTAGCT 
AAATTCCGCA AGCTCCAGCA TGAGCTGGAG GAGGCCGAGG AACGGGCTGA CATTGCTGAG 
TCCCAGGTGA ACAAACTGCG GGTGAAGAGC CGGGAGGTTC ACACAAAAGT CATAAGTGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MYH2 ( NM_001100112.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001093582.1 Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 2, mRNA.

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CloneID	OHu06054
Clone ID Related Accession (Same CDS sequence)	NM_017534.5 , NM_017534.6 , NM_001100112.1
Accession Version	NM_001100112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Homo sapiens(human)
Product	myosin-2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA765261.1, BC126409.1 and BC093082.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BX510904.2, AF111784.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1968189, SAMEA1968540 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCAG ACTCAGAATT GGCTGTTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAAACATC TGTCTTTGTG 
GCGGAGCCCA AAGAATCCTT TGTCAAAGGG ACCATCCAGA GCAGAGAAGG AGGAAAAGTG 
ACGGTGAAGA CTGAGGGAGG AGCGACTCTG ACAGTGAAGG ATGATCAGGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAT ATGGCCATGA TGACTCATCT GCATGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAACGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGT 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCTG TGTATAAGCC CGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGTG AGAAGAAGAA GGAAGAAATT ACTTCTGGCA AAATACAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGAATCC ACTTTGGCAC TACTGGAAAA 
CTGGCATCTG CTGATATTGA AACATATCTG CTAGAGAAGT CTAGAGTTGT TTTCCAGCTT 
AAGGCTGAGA GAAGTTATCA TATTTTTTAC CAGATTACAT CGAATAAGAA ACCAGAACTT 
ATTGAAATGC TTCTGATTAC CACGAACCCA TATGATTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CCAGCATCGA TGATCAGGAA GAACTGATGG CCACAGATAG TGCTATTGAT 
ATTTTGGGCT TTACTAATGA AGAAAAGGTC TCCATTTACA AGCTCACGGG GGCTGTGATG 
CATTATGGGA ACCTAAAATT TAAGCAAAAG CAGCGTGAGG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CTGACAAGGC GGCCTACCTC CAGAGTCTGA ACTCTGCAGA TCTGCTCAAA 
GCTCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTA 
GAACAGGTGT CCAACGCAGT AGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG TTTTGAGATT TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TCGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCCGA GGCCCACTTC 
GCTCTGATTC ACTATGCTGG TGTTGTGGAC TACAACATTA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTTGGA CTGTACCAGA AGTCTGCAAT GAAAACTCTA 
GCTCAGCTCT TCTCTGGGGC TCAAACTGCT GAAGGAGAGG GAGCTGGTGG AGGGGCCAAG 
AAAGGTGGTA AGAAGAAGGG CTCTTCTTTC CAGACAGTGT CTGCCCTTTT CAGAGAGAAT 
TTGAACAAGC TGATGACCAA CCTCAGGAGT ACCCATCCTC ACTTTGTGAG GTGTATCATC 
CCCAATGAGA CAAAAACTCC TGGTGCCATG GAGCATGAGC TTGTCCTCCA CCAGCTGAGG 
TGTAACGGTG TGCTGGAAGG CATCCGCATC TGTAGGAAAG GATTTCCAAG CAGAATCCTT 
TATGCAGACT TCAAACAGAG ATACAAGGTA TTAAATGCAA GTGCAATCCC TGAAGGGCAA 
TTCATTGATA GCAAGAAGGC CTCTGAGAAG CTCCTTGCAT CCATCGACAT TGACCACACC 
CAGTATAAAT TTGGGCACAC CAAGGTCTTT TTCAAAGCTG GTCTTCTGGG GCTCCTAGAG 
GAGATGCGAG ATGACAAGCT GGCCCAGCTG ATTACCCGAA CCCAGGCCAG GTGCAGAGGG 
TTCTTGGCAA GAGTGGAGTA CCAGAGGATG GTGGAGAGAA GGGAGGCCAT CTTCTGTATC 
CAGTACAATA TCAGATCCTT CATGAATGTC AAGCACTGGC CCTGGATGAA ACTCTTCTTC 
AAGATCAAGC CTCTGTTGAA GAGTGCAGAA ACTGAGAAGG AGATGGCCAC CATGAAGGAA 
GAATTTCAGA AAATTAAAGA CGAACTTGCC AAGTCAGAGG CAAAAAGGAA GGAACTGGAA 
GAAAAGATGG TGACGCTGTT GAAAGAAAAA AATGACTTGC AGCTCCAAGT TCAGGCTGAA 
GCCGAAGGCT TGGCTGATGC AGAGGAAAGG TGTGACCAGC TAATCAAAAC CAAAATCCAG 
CTAGAAGCCA AAATCAAAGA GGTGACTGAG AGAGCTGAGG ATGAGGAAGA GATCAATGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAACTCAA GAAAGACATT 
GATGACCTTG AGCTGACACT GGCCAAGGTT GAGAAGGAGA AACATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAAGA GATGGCAGGT CTGGATGAAA CCATTGCTAA GCTGACCAAG 
GAGAAGAAGG CTCTCCAGGA GGCCCACCAG CAGACCCTGG ATGACCTGCA GGCAGAGGAG 
GACAAAGTCA ACACCCTGAC CAAAGCTAAA ATCAAACTTG AACAACAAGT GGATGATCTT 
GAAGGGTCCT TGGAGCAAGA AAAGAAACTT CGCATGGACC TAGAAAGGGC TAAGAGGAAA 
CTTGAGGGTG ACTTGAAGTT GGCCCAAGAA TCCATAATGG ACATTGAAAA TGAGAAACAG 
CAACTTGATG AAAAGCTCAA AAAGAAAGAG TTTGAAATCA GCAATCTGCA AAGCAAGATT 
GAAGATGAAC AGGCACTTGG CATTCAATTG CAGAAGAAAA TTAAAGAATT GCAAGCCCGC 
ATTGAGGAGC TGGAGGAGGA AATCGAGGCA GAGCGGGCCT CCCGGGCCAA AGCAGAGAAG 
CAGCGCTCTG ACCTCTCCCG GGAGCTGGAG GAGATCAGCG AGAGGCTGGA AGAAGCCGGT 
GGGGCCACTT CAGCCCAGAT TGAGATGAAC AAGAAGCGGG AGGCTGAGTT CCAGAAAATG 
CGCAGGGACC TGGAGGAGGC CACCCTACAG CATGAAGCCA CAGCGGCCAC CCTGAGGAAG 
AAGCATGCAG ATAGTGTGGC CGAGCTTGGG GAGCAGATTG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGATG AAGATGGAGA TTGATGACCT TGCTAGTAAT 
GTAGAAACGG TCTCCAAAGC CAAGGGAAAC CTAGAGAAAA TGTGCCGGAC TCTAGAGGAC 
CAACTGAGTG AACTGAAATC AAAGGAAGAG GAGCAGCAGC GGCTGATCAA TGACCTGACT 
GCGCAGAGGG GGCGCCTGCA GACTGAATCT GGTGAGTTTT CACGCCAGCT TGATGAAAAG 
GAAGCTCTGG TGTCTCAGTT ATCAAGAGGC AAACAAGCCT TTACTCAACA GATTGAAGAA 
TTAAAGAGGC AACTTGAAGA GGAGATAAAA GCCAAGAACG CCCTGGCGCA TGCCCTGCAG 
TCTTCCCGCC ACGACTGTGA CCTGCTGCGG GAACAGTATG AGGAGGAGCA GGAATCCAAG 
GCCGAGCTGC AGAGAGCACT GTCCAAGGCC AACACCGAGG TTGCCCAATG GAGGACCAAA 
TACGAGACGG ACGCCATCCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCC 
CAGCGGCTGC AGGCAGCTGA GGAACATGTA GAAGCTGTGA ACGCCAAATG TGCTTCCCTC 
GAAAAGACGA AGCAGCGGCT GCAGAATGAG GTCGAGGACC TCATGCTTGA TGTGGAGAGG 
ACAAATGCCG CCTGTGCCGC CCTTGACAAA AAGCAAAGGA ACTTCGATAA GATCCTGGCA 
GAATGGAAAC AGAAATGTGA GGAAACGCAT GCTGAGCTTG AGGCCTCCCA GAAGGAGGCC 
CGTTCCCTTG GCACTGAGCT GTTCAAGATA AAGAATGCCT ATGAGGAATC TTTGGATCAG 
CTAGAAACCC TGAAGCGAGA GAACAAAAAC TTACAGCAGG AGATTTCTGA CCTCACGGAA 
CAGATTGCAG AAGGAGGGAA ACGTATCCAT GAACTGGAGA AAATAAAGAA ACAAGTGGAA 
CAAGAAAAGT GTGAACTTCA GGCTGCTTTA GAAGAAGCAG AGGCATCTCT TGAACATGAA 
GAGGGAAAGA TCCTGCGCAT CCAGCTTGAG TTGAACCAAG TCAAGTCTGA GGTTGATAGG 
AAAATTGCTG AAAAAGATGA GGAAATTGAC CAGCTGAAGA GAAACCACAT TAGAATCGTG 
GAGTCCATGC AGAGCACGCT GGATGCTGAG ATCAGGAGTA GGAATGATGC CATTAGGCTC 
AAGAAGAAGA TGGAGGGAGA CCTCAATGAA ATGGAAATCC AGCTGAACCA TGCCAACCGC 
ATGGCTGCTG AGGCCCTGAG GAACTACAGG AACACCCAAG GCATCCTCAA GGATACCCAG 
ATCCACCTGG ATGATGCTCT CCGGAGCCAG GAGGACCTGA AGGAACAGCT GGCCATGGTG 
GAGCGCAGAG CCAACCTGCT GCAGGCTGAG ATCGAGGAGC TGCGGGCCAC TCTGGAACAG 
ACAGAGAGGA GCAGAAAAAT CGCAGAACAG GAGCTCCTGG ATGCCAGTGA GCGTGTTCAG 
CTACTGCACA CCCAGAACAC CAGCCTGATC AACACCAAGA AGAAGCTGGA GACAGATATT 
TCCCAAATGC AAGGAGAGAT GGAGGACATT CTCCAGGAAG CCCGCAATGC AGAAGAAAAG 
GCCAAGAAGG CCATCACTGA TGCCGCCATG ATGGCTGAGG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAGCG GATGAAGAAG AACATGGAGC AGACCGTGAA GGATCTGCAG 
CTCCGTCTGG ATGAGGCTGA GCAGCTGGCC CTGAAGGGTG GGAAGAAGCA GATCCAGAAA 
CTGGAGGCCA GGGTACGGGA GCTGGAAGGA GAGGTTGAGA GTGAGCAAAA GCGTAATGCT 
GAGGCTGTCA AAGGTCTGCG CAAACATGAG AGGCGAGTGA AGGAACTCAC TTACCAGACG 
GAAGAAGATA GAAAGAATAT TCTCAGGCTT CAAGATTTGG TAGATAAACT TCAGGCAAAA 
GTGAAATCTT ATAAGAGACA AGCTGAGGAG GCTGAGGAAC AATCCAACAC CAATCTAGCT 
AAATTCCGCA AGCTCCAGCA TGAGCTGGAG GAGGCCGAGG AACGGGCTGA CATTGCTGAG 
TCCCAGGTGA ACAAACTGCG GGTGAAGAGC CGGGAGGTTC ACACAAAAGT CATAAGTGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093582.1
CDS	129..5954
Translation	MSSDSELAVFGEAAPFLRKSERERIEAQNRPFDAKTSVFVAEPKESFVKGTIQSREGGKVTVKTEGGATLTVKDDQVFPMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYKPEVVTAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGEKKKEEITSGKIQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVVFQLKAERSYHIFYQITSNKKPELIEMLLITTNPYDYPFVSQGEISVASIDDQEELMATDSAIDILGFTNEEKVSIYKLTGAVMHYGNLKFKQKQREEQAEPDGTEVADKAAYLQSLNSADLLKALCYPRVKVGNEYVTKGQTVEQVSNAVGALAKAVYEKMFLWMVARINQQLDTKQPRQYFIGVLDIAGFEIFDFNSLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGKSANFQKPKVVKGKAEAHFALIHYAGVVDYNITGWLEKNKDPLNETVVGLYQKSAMKTLAQLFSGAQTAEGEGAGGGAKKGGKKKGSSFQTVSALFRENLNKLMTNLRSTHPHFVRCIIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLASIDIDHTQYKFGHTKVFFKAGLLGLLEEMRDDKLAQLITRTQARCRGFLARVEYQRMVERREAIFCIQYNIRSFMNVKHWPWMKLFFKIKPLLKSAETEKEMATMKEEFQKIKDELAKSEAKRKELEEKMVTLLKEKNDLQLQVQAEAEGLADAEERCDQLIKTKIQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDETIAKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKIKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESIMDIENEKQQLDEKLKKKEFEISNLQSKIEDEQALGIQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAATLRKKHADSVAELGEQIDNLQRVKQKLEKEKSEMKMEIDDLASNVETVSKAKGNLEKMCRTLEDQLSELKSKEEEQQRLINDLTAQRGRLQTESGEFSRQLDEKEALVSQLSRGKQAFTQQIEELKRQLEEEIKAKNALAHALQSSRHDCDLLREQYEEEQESKAELQRALSKANTEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQAAEEHVEAVNAKCASLEKTKQRLQNEVEDLMLDVERTNAACAALDKKQRNFDKILAEWKQKCEETHAELEASQKEARSLGTELFKIKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKRIHELEKIKKQVEQEKCELQAALEEAEASLEHEEGKILRIQLELNQVKSEVDRKIAEKDEEIDQLKRNHIRIVESMQSTLDAEIRSRNDAIRLKKKMEGDLNEMEIQLNHANRMAAEALRNYRNTQGILKDTQIHLDDALRSQEDLKEQLAMVERRANLLQAEIEELRATLEQTERSRKIAEQELLDASERVQLLHTQNTSLINTKKKLETDISQMQGEMEDILQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQLRLDEAEQLALKGGKKQIQKLEARVRELEGEVESEQKRNAEAVKGLRKHERRVKELTYQTEEDRKNILRLQDLVDKLQAKVKSYKRQAEEAEEQSNTNLAKFRKLQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	NM_001100112.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myosin heavy chain 2 (MYH2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100112.1

ORF Insert Sequence:

ATGAGTTCAG ACTCAGAATT GGCTGTTTTT GGGGAGGCTG CTCCTTTCCT CCGAAAGTCT 
GAAAGGGAGC GCATTGAGGC CCAGAATAGG CCCTTTGATG CCAAAACATC TGTCTTTGTG 
GCGGAGCCCA AAGAATCCTT TGTCAAAGGG ACCATCCAGA GCAGAGAAGG AGGAAAAGTG 
ACGGTGAAGA CTGAGGGAGG AGCGACTCTG ACAGTGAAGG ATGATCAGGT CTTCCCCATG 
AACCCTCCCA AATATGACAA GATCGAGGAT ATGGCCATGA TGACTCATCT GCATGAGCCT 
GCTGTGCTGT ACAACCTCAA AGAACGTTAT GCAGCCTGGA TGATCTACAC CTATTCAGGT 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGCTGCCTG TGTATAAGCC CGAGGTGGTG 
ACAGCCTACC GAGGCAAAAA GCGCCAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAC 
AACGCCTATC AGTTCATGCT GACTGACCGA GAGAATCAGT CAATCCTGAT CACTGGAGAA 
TCTGGTGCAG GGAAGACTGT GAACACCAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTTACTGGTG AGAAGAAGAA GGAAGAAATT ACTTCTGGCA AAATACAGGG GACTCTGGAA 
GATCAAATCA TCAGTGCCAA CCCCCTACTG GAGGCCTTTG GCAACGCCAA GACCGTGAGG 
AATGACAACT CCTCTCGCTT TGGTAAATTC ATCAGAATCC ACTTTGGCAC TACTGGAAAA 
CTGGCATCTG CTGATATTGA AACATATCTG CTAGAGAAGT CTAGAGTTGT TTTCCAGCTT 
AAGGCTGAGA GAAGTTATCA TATTTTTTAC CAGATTACAT CGAATAAGAA ACCAGAACTT 
ATTGAAATGC TTCTGATTAC CACGAACCCA TATGATTACC CATTTGTCAG TCAAGGGGAG 
ATCAGTGTGG CCAGCATCGA TGATCAGGAA GAACTGATGG CCACAGATAG TGCTATTGAT 
ATTTTGGGCT TTACTAATGA AGAAAAGGTC TCCATTTACA AGCTCACGGG GGCTGTGATG 
CATTATGGGA ACCTAAAATT TAAGCAAAAG CAGCGTGAGG AGCAAGCAGA GCCAGATGGC 
ACAGAAGTTG CTGACAAGGC GGCCTACCTC CAGAGTCTGA ACTCTGCAGA TCTGCTCAAA 
GCTCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTATG TCACCAAAGG CCAGACTGTA 
GAACAGGTGT CCAACGCAGT AGGTGCTCTG GCCAAAGCCG TCTACGAGAA GATGTTCCTG 
TGGATGGTTG CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATTGCTGG TTTTGAGATT TTTGATTTCA ACAGCCTGGA GCAGCTGTGC 
ATCAACTTCA CCAATGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATCGAG TGGACGTTCA TCGACTTCGG GATGGACCTG 
GCTGCCTGCA TCGAGCTCAT CGAGAAGCCT ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CTAAGGCAAC AGACACCTCC TTCAAGAACA AGCTGTATGA CCAGCACCTG 
GGCAAGTCTG CCAACTTCCA GAAGCCCAAG GTGGTCAAAG GCAAGGCCGA GGCCCACTTC 
GCTCTGATTC ACTATGCTGG TGTTGTGGAC TACAACATTA CTGGCTGGCT GGAGAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTTGGA CTGTACCAGA AGTCTGCAAT GAAAACTCTA 
GCTCAGCTCT TCTCTGGGGC TCAAACTGCT GAAGGAGAGG GAGCTGGTGG AGGGGCCAAG 
AAAGGTGGTA AGAAGAAGGG CTCTTCTTTC CAGACAGTGT CTGCCCTTTT CAGAGAGAAT 
TTGAACAAGC TGATGACCAA CCTCAGGAGT ACCCATCCTC ACTTTGTGAG GTGTATCATC 
CCCAATGAGA CAAAAACTCC TGGTGCCATG GAGCATGAGC TTGTCCTCCA CCAGCTGAGG 
TGTAACGGTG TGCTGGAAGG CATCCGCATC TGTAGGAAAG GATTTCCAAG CAGAATCCTT 
TATGCAGACT TCAAACAGAG ATACAAGGTA TTAAATGCAA GTGCAATCCC TGAAGGGCAA 
TTCATTGATA GCAAGAAGGC CTCTGAGAAG CTCCTTGCAT CCATCGACAT TGACCACACC 
CAGTATAAAT TTGGGCACAC CAAGGTCTTT TTCAAAGCTG GTCTTCTGGG GCTCCTAGAG 
GAGATGCGAG ATGACAAGCT GGCCCAGCTG ATTACCCGAA CCCAGGCCAG GTGCAGAGGG 
TTCTTGGCAA GAGTGGAGTA CCAGAGGATG GTGGAGAGAA GGGAGGCCAT CTTCTGTATC 
CAGTACAATA TCAGATCCTT CATGAATGTC AAGCACTGGC CCTGGATGAA ACTCTTCTTC 
AAGATCAAGC CTCTGTTGAA GAGTGCAGAA ACTGAGAAGG AGATGGCCAC CATGAAGGAA 
GAATTTCAGA AAATTAAAGA CGAACTTGCC AAGTCAGAGG CAAAAAGGAA GGAACTGGAA 
GAAAAGATGG TGACGCTGTT GAAAGAAAAA AATGACTTGC AGCTCCAAGT TCAGGCTGAA 
GCCGAAGGCT TGGCTGATGC AGAGGAAAGG TGTGACCAGC TAATCAAAAC CAAAATCCAG 
CTAGAAGCCA AAATCAAAGA GGTGACTGAG AGAGCTGAGG ATGAGGAAGA GATCAATGCT 
GAGCTGACAG CCAAGAAGAG GAAACTGGAG GATGAATGTT CAGAACTCAA GAAAGACATT 
GATGACCTTG AGCTGACACT GGCCAAGGTT GAGAAGGAGA AACATGCCAC AGAAAACAAG 
GTGAAAAACC TCACAGAAGA GATGGCAGGT CTGGATGAAA CCATTGCTAA GCTGACCAAG 
GAGAAGAAGG CTCTCCAGGA GGCCCACCAG CAGACCCTGG ATGACCTGCA GGCAGAGGAG 
GACAAAGTCA ACACCCTGAC CAAAGCTAAA ATCAAACTTG AACAACAAGT GGATGATCTT 
GAAGGGTCCT TGGAGCAAGA AAAGAAACTT CGCATGGACC TAGAAAGGGC TAAGAGGAAA 
CTTGAGGGTG ACTTGAAGTT GGCCCAAGAA TCCATAATGG ACATTGAAAA TGAGAAACAG 
CAACTTGATG AAAAGCTCAA AAAGAAAGAG TTTGAAATCA GCAATCTGCA AAGCAAGATT 
GAAGATGAAC AGGCACTTGG CATTCAATTG CAGAAGAAAA TTAAAGAATT GCAAGCCCGC 
ATTGAGGAGC TGGAGGAGGA AATCGAGGCA GAGCGGGCCT CCCGGGCCAA AGCAGAGAAG 
CAGCGCTCTG ACCTCTCCCG GGAGCTGGAG GAGATCAGCG AGAGGCTGGA AGAAGCCGGT 
GGGGCCACTT CAGCCCAGAT TGAGATGAAC AAGAAGCGGG AGGCTGAGTT CCAGAAAATG 
CGCAGGGACC TGGAGGAGGC CACCCTACAG CATGAAGCCA CAGCGGCCAC CCTGAGGAAG 
AAGCATGCAG ATAGTGTGGC CGAGCTTGGG GAGCAGATTG ACAACCTGCA GCGAGTGAAG 
CAGAAGCTGG AGAAGGAGAA GAGTGAGATG AAGATGGAGA TTGATGACCT TGCTAGTAAT 
GTAGAAACGG TCTCCAAAGC CAAGGGAAAC CTAGAGAAAA TGTGCCGGAC TCTAGAGGAC 
CAACTGAGTG AACTGAAATC AAAGGAAGAG GAGCAGCAGC GGCTGATCAA TGACCTGACT 
GCGCAGAGGG GGCGCCTGCA GACTGAATCT GGTGAGTTTT CACGCCAGCT TGATGAAAAG 
GAAGCTCTGG TGTCTCAGTT ATCAAGAGGC AAACAAGCCT TTACTCAACA GATTGAAGAA 
TTAAAGAGGC AACTTGAAGA GGAGATAAAA GCCAAGAACG CCCTGGCGCA TGCCCTGCAG 
TCTTCCCGCC ACGACTGTGA CCTGCTGCGG GAACAGTATG AGGAGGAGCA GGAATCCAAG 
GCCGAGCTGC AGAGAGCACT GTCCAAGGCC AACACCGAGG TTGCCCAATG GAGGACCAAA 
TACGAGACGG ACGCCATCCA GCGCACAGAG GAGCTGGAGG AGGCCAAGAA GAAGCTGGCC 
CAGCGGCTGC AGGCAGCTGA GGAACATGTA GAAGCTGTGA ACGCCAAATG TGCTTCCCTC 
GAAAAGACGA AGCAGCGGCT GCAGAATGAG GTCGAGGACC TCATGCTTGA TGTGGAGAGG 
ACAAATGCCG CCTGTGCCGC CCTTGACAAA AAGCAAAGGA ACTTCGATAA GATCCTGGCA 
GAATGGAAAC AGAAATGTGA GGAAACGCAT GCTGAGCTTG AGGCCTCCCA GAAGGAGGCC 
CGTTCCCTTG GCACTGAGCT GTTCAAGATA AAGAATGCCT ATGAGGAATC TTTGGATCAG 
CTAGAAACCC TGAAGCGAGA GAACAAAAAC TTACAGCAGG AGATTTCTGA CCTCACGGAA 
CAGATTGCAG AAGGAGGGAA ACGTATCCAT GAACTGGAGA AAATAAAGAA ACAAGTGGAA 
CAAGAAAAGT GTGAACTTCA GGCTGCTTTA GAAGAAGCAG AGGCATCTCT TGAACATGAA 
GAGGGAAAGA TCCTGCGCAT CCAGCTTGAG TTGAACCAAG TCAAGTCTGA GGTTGATAGG 
AAAATTGCTG AAAAAGATGA GGAAATTGAC CAGCTGAAGA GAAACCACAT TAGAATCGTG 
GAGTCCATGC AGAGCACGCT GGATGCTGAG ATCAGGAGTA GGAATGATGC CATTAGGCTC 
AAGAAGAAGA TGGAGGGAGA CCTCAATGAA ATGGAAATCC AGCTGAACCA TGCCAACCGC 
ATGGCTGCTG AGGCCCTGAG GAACTACAGG AACACCCAAG GCATCCTCAA GGATACCCAG 
ATCCACCTGG ATGATGCTCT CCGGAGCCAG GAGGACCTGA AGGAACAGCT GGCCATGGTG 
GAGCGCAGAG CCAACCTGCT GCAGGCTGAG ATCGAGGAGC TGCGGGCCAC TCTGGAACAG 
ACAGAGAGGA GCAGAAAAAT CGCAGAACAG GAGCTCCTGG ATGCCAGTGA GCGTGTTCAG 
CTACTGCACA CCCAGAACAC CAGCCTGATC AACACCAAGA AGAAGCTGGA GACAGATATT 
TCCCAAATGC AAGGAGAGAT GGAGGACATT CTCCAGGAAG CCCGCAATGC AGAAGAAAAG 
GCCAAGAAGG CCATCACTGA TGCCGCCATG ATGGCTGAGG AGCTGAAGAA GGAGCAGGAC 
ACCAGCGCCC ACCTGGAGCG GATGAAGAAG AACATGGAGC AGACCGTGAA GGATCTGCAG 
CTCCGTCTGG ATGAGGCTGA GCAGCTGGCC CTGAAGGGTG GGAAGAAGCA GATCCAGAAA 
CTGGAGGCCA GGGTACGGGA GCTGGAAGGA GAGGTTGAGA GTGAGCAAAA GCGTAATGCT 
GAGGCTGTCA AAGGTCTGCG CAAACATGAG AGGCGAGTGA AGGAACTCAC TTACCAGACG 
GAAGAAGATA GAAAGAATAT TCTCAGGCTT CAAGATTTGG TAGATAAACT TCAGGCAAAA 
GTGAAATCTT ATAAGAGACA AGCTGAGGAG GCTGAGGAAC AATCCAACAC CAATCTAGCT 
AAATTCCGCA AGCTCCAGCA TGAGCTGGAG GAGGCCGAGG AACGGGCTGA CATTGCTGAG 
TCCCAGGTGA ACAAACTGCG GGTGAAGAGC CGGGAGGTTC ACACAAAAGT CATAAGTGAA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Congenital myopathy due to myosin heavy chain 2 mutation presenting as chronic aspiration pneumonia in infancy.
Neuromuscular disorders : NMD27(10)947-950(2017 Oct)
Tsabari R,Daum H,Kerem E,Fellig Y,Dor T

Myosin IIA Heavy Chain Phosphorylation Mediates Adhesion Maturation and Protrusion in Three Dimensions.
The Journal of biological chemistry292(8)3099-3111(2017 02)
Rai V,Thomas DG,Beach JR,Egelhoff TT

Confinement Sensing and Signal Optimization via Piezo1/PKA and Myosin II Pathways.
Cell reports15(7)1430-1441(2016 05)
Hung WC,Yang JR,Yankaskas CL,Wong BS,Wu PH,Pardo-Pastor C,Serra SA,Chiang MJ,Gu Z,Wirtz D,Valverde MA,Yang JT,Zhang J,Konstantopoulos K

Transition of myosin heavy chain isoforms in human laryngeal abductors following denervation.
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery272(10)2915-23(2015 Oct)
Qiu X,Chen D,Li M,Gao Y,Liu F,Zheng H,Chen S

Mutations and sequence variation in the human myosin heavy chain IIa gene (MYH2).
European journal of human genetics : EJHG13(5)617-22(2005 May)
Tajsharghi H,Darin N,Rekabdar E,Kyllerman M,Wahlstr?m J,Martinsson T,Oldfors A

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MYH2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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