ASTN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASTN1
Entrez Gene ID	460
Full Name	astrotactin 1
Synonyms	ASTN
General protein information	Preferred Names astrotactin 1 Names astrotactin-1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q25.2
Summary	Astrotactin is a neuronal adhesion molecule required for glial-guided migration of young postmitotic neuroblasts in cortical regions of developing brain, including cerebrum, hippocampus, cerebellum, and olfactory bulb (Fink et al., 1995).[supplied by OMIM, Jun 2009].
Disorder MIM:	600904

mRNA and Protein(s)

mRNA	Protein	Name
NM_001364856.1	NP_001351785.1	astrotactin-1 isoform 4 precursor
NM_004319.2	NP_004310.1	astrotactin-1 isoform 1 precursor
NM_004319.3	NP_004310.1	astrotactin-1 isoform 1 precursor
XM_017001341.2	XP_016856830.1	astrotactin-1 isoform X2
XM_017001341.1	XP_016856830.1	astrotactin-1 isoform X2
NM_207108.2	NP_996991.1	astrotactin-1 isoform 2 precursor
NM_001286164.1	NP_001273093.1	astrotactin-1 isoform 3 precursor
XM_017001340.1	XP_016856829.1	astrotactin-1 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	ASTN1	NP_004310.1
Canis lupus familiaris (dog)	ASTN1	XP_005622546.1
Bos taurus (cattle)	ASTN1	XP_002694147.2
Pan troglodytes (chimpanzee)	ASTN1	XP_514021.3
	ASTN1	XP_001105186.1
Xenopus tropicalis (tropical clawed frog)	astn1	NP_001072522.1
Mus musculus (house mouse)	Astn1	NP_031521.2
Rattus norvegicus (Norway rat)	Astn1	NP_001164074.1
Gallus gallus (chicken)	ASTN1	XP_004943315.1
Danio rerio (zebrafish)	astn1	XP_003197930.2

Gene Ontology
Bibliography

Related articles in PubMed

ASTN1 and alcohol dependence: family-based association analysis in multiplex alcohol dependence families.
Hill SY, Weeks DE, Jones BL, Zezza N, Stiffler S
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics159B(4)445-55(2012 Jun)

Identification of new putative susceptibility genes for several psychiatric disorders by association analysis of regulatory and non-synonymous SNPs of 306 genes involved in neurotransmission and neurodevelopment.
Gratac?s M, Costas J, de Cid R, Bay?s M, Gonz?lez JR, Baca-Garc?a E, de Diego Y, Fern?ndez-Aranda F, Fern?ndez-Piqueras J, Guitart M, Mart?n-Santos R, Martorell L, Mench?n JM, Roca M, S?iz-Ruiz J, Sanju?n J, Torrens M, Urretavizcaya M, Valero J, Vilella E, Estivill X, Carracedo A,
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics150B(6)808-16(2009 Sep)

Association analysis of schizophrenia on 18 genes involved in neuronal migration: MDGA1 as a new susceptibility gene.
K?hler AK, Djurovic S, Kulle B, J?nsson EG, Agartz I, Hall H, Opjordsmoen S, Jakobsen KD, Hansen T, Melle I, Werge T, Steen VM, Andreassen OA
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics147B(7)1089-100(2008 Oct)

Molecular genetics of successful smoking cessation: convergent genome-wide association study results.
Uhl GR, Liu QR, Drgon T, Johnson C, Walther D, Rose JE, David SP, Niaura R, Lerman C
Archives of general psychiatry65(6)683-93(2008 Jun)

Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins.
Ohara O, Nagase T, Ishikawa K, Nakajima D, Ohira M, Seki N, Nomura N
DNA research : an international journal for rapid publication of reports on genes and genomes4(1)53-9(1997 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Family-based association analysis shows the ASTN1 gene significantly associated with alcohol dependence.
Title: ASTN1 and alcohol dependence: family-based association analysis in multiplex alcohol dependence families.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Observational study of gene-disease association. (HuGE Navigator)
Title: Identification of new putative susceptibility genes for several psychiatric disorders by association analysis of regulatory and non-synonymous SNPs of 306 genes involved in neurotransmission and neurodevelopment.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association analysis of schizophrenia on 18 genes involved in neuronal migration: MDGA1 as a new susceptibility gene.

Clinical trial and genome-wide association study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Molecular genetics of successful smoking cessation: convergent genome-wide association study results.

The following ASTN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASTN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14883	NM_004319.3 Latest version!	Homo sapiens astrotactin 1 (ASTN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu14964	NM_001286164.1 Latest version!	Homo sapiens astrotactin 1 (ASTN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu14967	NM_207108.2 Latest version!	Homo sapiens astrotactin 1 (ASTN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$860.30 $1229.00
OHu90047	NM_001364856.1 Latest version!	Homo sapiens astrotactin 1 (ASTN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu90047	XM_017001340.1 Latest version!	Homo sapiens astrotactin 1 (ASTN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu90048	XM_017001341.2 Latest version!	Homo sapiens astrotactin 1 (ASTN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu14883	NM_004319.2	Homo sapiens astrotactin 1 (ASTN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu90048	XM_017001341.1	Homo sapiens astrotactin 1 (ASTN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASTN1 ( NM_004319.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_004310.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14883
Clone ID Related Accession (Same CDS sequence)	NM_004319.2 , NM_004319.3
Accession Version	NM_004319.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL021398.1, BC146795.1, AL031290.1 and BF507471.1. On Oct 31, 2013 this sequence version replaced NM_004319.1. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC146795.1, SRR1803617.73824.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGAA AGCTGGTCTC 
ATCCTTTCCC AGCTTGGGGA CCTCAGCAGT TGGTGCAATG GACTCCTTCA GGAACCCAAG 
ATAAGCTTGC GGCGCAGCTC ACTCAAGTAC CTGGGGTGCC GCTACAGCGA GATCAAACCC 
TACGGACTTG ACTGGGCGGA GCTCAGCCGG GACCTCAGGA AGACGTGTGA GGAGCAGACC 
CTGAGTATCC CCTACAACGA CTATGGGGAC AGCAAAGAGA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004310.1
CDS	229..4113
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPRKAGLILSQLGDLSSWCNGLLQEPKISLRRSSLKYLGCRYSEIKPYGLDWAELSRDLRKTCEEQTLSIPYNDYGDSKEI

Target ORF information:

RefSeq Version	NM_004319.2
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004319.2

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGAA AGCTGGTCTC 
ATCCTTTCCC AGCTTGGGGA CCTCAGCAGT TGGTGCAATG GACTCCTTCA GGAACCCAAG 
ATAAGCTTGC GGCGCAGCTC ACTCAAGTAC CTGGGGTGCC GCTACAGCGA GATCAAACCC 
TACGGACTTG ACTGGGCGGA GCTCAGCCGG GACCTCAGGA AGACGTGTGA GGAGCAGACC 
CTGAGTATCC CCTACAACGA CTATGGGGAC AGCAAAGAGA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTN1 ( NM_004319.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004310.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14883
Clone ID Related Accession (Same CDS sequence)	NM_004319.2 , NM_004319.3
Accession Version	NM_004319.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3885bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform 1 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL021398.1, BC146795.1, AL031290.1 and BF507471.1. On Nov 23, 2018 this sequence version replaced NM_004319.2. Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC146795.1, SRR1803617.73824.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361833.7/ ENSP00000354536.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGAA AGCTGGTCTC 
ATCCTTTCCC AGCTTGGGGA CCTCAGCAGT TGGTGCAATG GACTCCTTCA GGAACCCAAG 
ATAAGCTTGC GGCGCAGCTC ACTCAAGTAC CTGGGGTGCC GCTACAGCGA GATCAAACCC 
TACGGACTTG ACTGGGCGGA GCTCAGCCGG GACCTCAGGA AGACGTGTGA GGAGCAGACC 
CTGAGTATCC CCTACAACGA CTATGGGGAC AGCAAAGAGA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004310.1
CDS	37..3921
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPRKAGLILSQLGDLSSWCNGLLQEPKISLRRSSLKYLGCRYSEIKPYGLDWAELSRDLRKTCEEQTLSIPYNDYGDSKEI

Target ORF information:

RefSeq Version	NM_004319.3
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004319.3

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGAA AGCTGGTCTC 
ATCCTTTCCC AGCTTGGGGA CCTCAGCAGT TGGTGCAATG GACTCCTTCA GGAACCCAAG 
ATAAGCTTGC GGCGCAGCTC ACTCAAGTAC CTGGGGTGCC GCTACAGCGA GATCAAACCC 
TACGGACTTG ACTGGGCGGA GCTCAGCCGG GACCTCAGGA AGACGTGTGA GGAGCAGACC 
CTGAGTATCC CCTACAACGA CTATGGGGAC AGCAAAGAGA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTN1 ( NM_001286164.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273093.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant 3, mRNA.

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CloneID	OHu14964
Clone ID Related Accession (Same CDS sequence)	NM_001286164.1
Accession Version	NM_001286164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3687bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform 3 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL021398.1, BC032870.1, AL022145.1 and AL031290.1. On Oct 31, 2013 this sequence version replaced XM_005245195.1. Transcript Variant: This variant (3) differs in the 3' UTR and uses an alternate exon in the 3' coding region. The encoded isoform (3) is shorter and has a distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC032870.1, SRR1803615.209661.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGCT TCCAGCGTGT 
TGGGACAGTC AGAGGAGTTG TAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273093.1
CDS	229..3915
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPRLPACWDSQRSCR

Target ORF information:

RefSeq Version	NM_001286164.1
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286164.1

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGCT TCCAGCGTGT 
TGGGACAGTC AGAGGAGTTG TAGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTN1 ( NM_207108.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_996991.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant 2, mRNA.

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CloneID	OHu14967
Clone ID Related Accession (Same CDS sequence)	NM_207108.2
Accession Version	NM_207108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform 2 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL021398.1, BC142697.1 and AL031290.1. On Oct 31, 2013 this sequence version replaced NM_207108.1. Transcript Variant: This variant (2) uses a different splice site in the 3' coding region and 3' UTR, compared to variant 1. The resulting protein (isoform 2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BC142697.1, AK295126.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_996991.1
CDS	229..3879
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPR

Target ORF information:

RefSeq Version	NM_207108.2
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207108.2

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGG 
GAATGCCTCT GCTATGAAGG CTACATGAAG GATCCAGTAC ATAAGCACCT TTGCATTCGG 
AACGAATGGG GGACAAACCA GGGGCCATGG CCTTACACAA TATTTCAGCG AGGCTTTGAC 
CTGGTTTTGG GAGAGCAGCC CTCTGATAAA ATATTTAGAT TCACCTACAC TCTTGGGGAG 
GGCATGTGGT TGCCCCTCAG CAAGAGCTTT GTGATTCCAC CAGCCGAACT GGCCATCAAT 
CCATCAGCAA AGTGCAAGAC GGACATGACT GTGATGGAGG ATGCTGTGGA GGTCAGAGAG 
GAGCTGATGA CTTCATCCTC CTTCGACAGC CTGGAGGTTC TCTTAGATTC CTTTGGGCCG 
GTGCGCGACT GCAGCAAAGA TAACGGGGGC TGCAGTAAGA ATTTCCGCTG TATTTCAGAT 
CGCAAGCTGG ACTCCACTGG TTGCGTGTGC CCATCTGGAC TCAGTCCCAT GAAGGACAGC 
TCTGGCTGCT ATGACCGCCA CATCGGGGTG GACTGTTCCG ACGGCTTCAA CGGCGGCTGT 
GAGCAGCTGT GCCTCCAGCA GATGGCGCCC TTCCCGGACG ACCCCACCTT GTATAACATC 
CTCATGTTCT GTGGGTGCAT CGAGGACTAC AAGCTTGGTG TGGATGGACG CTCTTGCCAA 
CTCATCACGG AGACCTGTCC AGAGGGAAGT GACTGTGGGG AAAGCAGGGA GCTTCCCATG 
AACCAGACCC TCTTTGGGGA GATGTTCTTT GGTTACAACA ACCATTCCAA GGAAGTGGCT 
GCCGGACAGG TGCTGAAAGG AACATTCAGG CAAAACAACT TTGCTCGTGG TTTAGACCAG 
CAACTGCCAG ATGGTCTTGT GGTGGCCACT GTGCCCCTGG AGAATCAATG CCTAGAGGAG 
ATCTCGGAGC CCACCCCTGA CCCTGACTTC CTGACTGGGA TGGTGAACTT CAGTGAAGTG 
TCTGGGTACC CTGTGCTGCA GCACTGGAAG GTCCGGTCTG TGATGTACCA CATCAAACTC 
AACCAAGTGG CCATCTCTCA GGCCCTCAGC AATGCTCTCC ACTCGCTGGA TGGGGCTACA 
TCTCGTGCAG ATTTTGTGGC GCTGTTGGAC CAGTTCGGCA ACCATTACAT CCAGGAAGCT 
ATCTACGGCT TTGAGGAGTC CTGTTCTATC TGGTACCCAA ACAAGCAGGT CCAGCGGCGA 
CTCTGGCTGG AGTATGAAGA CATCAGTAAA GGCAACTCCC CATCAGATGA GTCTGAGGAG 
CGGGAAAGAG ACCCCAAGGT GCTGACATTC CCAGAATACA TCACCAGCTT GTCAGACTCC 
GGCACCAAGC ACATGGCGGC TGGAGTCCGC ATGGAGTGCC ACAGCAAGGG ACGATGCCCC 
TCGTCCTGCC CCCTGTGTCA TGTGACATCC AGCCCTGACA CCCCTGCTGA GCCGGTTCTG 
CTGGAGGTGA CCAAAGCAGC CCCCATCTAT GAACTAGTGA CCAACAACCA GACCCAGAGG 
CTCTTGCAGG AGGCTACCAT GAGCTCTCTC TGGTGCTCAG GGACTGGAGA TGTCATCGAG 
GACTGGTGTC GATGTGACTC CACTGCTTTT GGAGCTGATG GACTCCCCAC CTGTGCGCCT 
CTCCCACAGC CTGTGCTGAG ACTCTCCACG GTTCACGAGC CCAGCAGCAC TCTTGTGGTC 
CTGGAGTGGG AACACTCAGA GCCACCAATC GGGGTGCAGA TTGTAGATTA CCTCCTCCGT 
CAAGAGAAAG TCACTGACAG GATGGACCAC TCCAAAGTGG AGACAGAAAC AGTGCTGAGC 
TTTGTGGACG ACATCATCTC TGGAGCAAAG TCTCCTTGTG CAATGCCATC TCAGGTGCCG 
GACAAGCAGC TCACCACCAT CTCTCTGATC ATACGATGCC TGGAACCTGA CACCATTTAC 
ATGTTCACGC TGTGGGGAGT GGACAACACA GGACGGCGCT CCAGGCCAAG CGACGTGATC 
GTGAAGACCC CATGCCCCGT GGTGGATGAT GTCAAGGCTC AAGAAATAGC AGACAAGATC 
TACAATCTCT TCAATGGCTA CACTAGTGGG AAGGAGCAGC AGACCGCCTA CAACACCCTC 
CTGGATCTGG GTTCCCCCAC CTTACACCGG GTCCTCTACC ACTATAACCA GCACTATGAG 
AGTTTTGGGG AATTCACCTG GCGATGTGAG GATGAGTTAG GTCCCAGGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTN1 ( NM_001364856.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001351785.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant 4, mRNA.

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CloneID	OHu90047
Clone ID Related Accession (Same CDS sequence)	NM_001364856.1 , XM_017001340.1
Accession Version	NM_001364856.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-01
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform 4 precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL021398.1, AL136983.11, AL022145.1 and AL031290.1. On Jul 12, 2018 this sequence version replaced XM_017001340.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.147409.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGAAAGCTGG TCTCATCCTT TCCCAGCTTG GGGACCTCAG CAGTTGGTGC 
AATGGACTCC TTCAGGAACC CAAGATAAGC TTGCGGCGCA GCTCACTCAA GTACCTGGGG 
TGCCGCTACA GCGAGATCAA ACCCTACGGA CTTGACTGGG CGGAGCTCAG CCGGGACCTC 
AGGAAGACGT GTGAGGAGCA GACCCTGAGT ATCCCCTACA ACGACTATGG GGACAGCAAA 
GAGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001351785.1
CDS	37..3945
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGRREHRAAGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPRKAGLILSQLGDLSSWCNGLLQEPKISLRRSSLKYLGCRYSEIKPYGLDWAELSRDLRKTCEEQTLSIPYNDYGDSKEI

Target ORF information:

RefSeq Version	NM_001364856.1
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001364856.1

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGAAAGCTGG TCTCATCCTT TCCCAGCTTG GGGACCTCAG CAGTTGGTGC 
AATGGACTCC TTCAGGAACC CAAGATAAGC TTGCGGCGCA GCTCACTCAA GTACCTGGGG 
TGCCGCTACA GCGAGATCAA ACCCTACGGA CTTGACTGGG CGGAGCTCAG CCGGGACCTC 
AGGAAGACGT GTGAGGAGCA GACCCTGAGT ATCCCCTACA ACGACTATGG GGACAGCAAA 
GAGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTN1 ( XM_017001340.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856829.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant X1, mRNA.

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CloneID	OHu90047
Clone ID Related Accession (Same CDS sequence)	NM_001364856.1 , XM_017001340.1
Accession Version	XM_017001340.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGAAAGCTGG TCTCATCCTT TCCCAGCTTG GGGACCTCAG CAGTTGGTGC 
AATGGACTCC TTCAGGAACC CAAGATAAGC TTGCGGCGCA GCTCACTCAA GTACCTGGGG 
TGCCGCTACA GCGAGATCAA ACCCTACGGA CTTGACTGGG CGGAGCTCAG CCGGGACCTC 
AGGAAGACGT GTGAGGAGCA GACCCTGAGT ATCCCCTACA ACGACTATGG GGACAGCAAA 
GAGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856829.1
CDS	179..4087
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGRREHRAAGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPRKAGLILSQLGDLSSWCNGLLQEPKISLRRSSLKYLGCRYSEIKPYGLDWAELSRDLRKTCEEQTLSIPYNDYGDSKEI

Target ORF information:

RefSeq Version	XM_017001340.1
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001340.1

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGAAAGCTGG TCTCATCCTT TCCCAGCTTG GGGACCTCAG CAGTTGGTGC 
AATGGACTCC TTCAGGAACC CAAGATAAGC TTGCGGCGCA GCTCACTCAA GTACCTGGGG 
TGCCGCTACA GCGAGATCAA ACCCTACGGA CTTGACTGGG CGGAGCTCAG CCGGGACCTC 
AGGAAGACGT GTGAGGAGCA GACCCTGAGT ATCCCCTACA ACGACTATGG GGACAGCAAA 
GAGATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTN1 ( XM_017001341.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856830.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant X2, mRNA.

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CloneID	OHu90048
Clone ID Related Accession (Same CDS sequence)	XM_017001341.2 , XM_017001341.1
Accession Version	XM_017001341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017001341.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGCTTCCAGC GTGTTGGGAC AGTCAGAGGA GTTGTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856830.1
CDS	179..3889
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGRREHRAAGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPRLPACWDSQRSCR

Target ORF information:

RefSeq Version	XM_017001341.2
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001341.2

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGCTTCCAGC GTGTTGGGAC AGTCAGAGGA GTTGTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASTN1 ( XM_017001341.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016856830.1 Homo sapiens astrotactin 1 (ASTN1), transcript variant X2, mRNA.

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CloneID	OHu90048
Clone ID Related Accession (Same CDS sequence)	XM_017001341.2 , XM_017001341.1
Accession Version	XM_017001341.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	astrotactin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGCTTCCAGC GTGTTGGGAC AGTCAGAGGA GTTGTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016856830.1
CDS	179..3889
Translation	MALAGLCALLACCWGPAAVLATAAGDVDPSKELECKLKSITVSALPFLRENDLSIMHSPSASEPKLLFSVRNDFPGEMVVVDDLENTELPYFVLEISGNTEDIPLVRWRQQWLENGTLLFHIHHQDGAPSLPGQDPTEEPQHESAEEELRILHISVMGGMIALLLSILCLVMILYTRRRWCKRRRVPQPQKSASAEAANEIHYIPSVLIGGHGRESLRNARVQGHNSSGTLSIRETPILDGYEYDITDLRHHLQRECMNGGEDFASQVTRTLDSLQGCNEKSGMDLTPGSDNAKLSLMNKYKDNIIATSPVDSNHQQATLLSHTSSSQRKRINNKARAGSAFLNPEGDSGTEAENDPQLTFYTDPSRSRRRSRVGSPRSPVNKTTLTLISITSCVIGLVCSSHVNCPLVVKITLHVPEHLIADGSRFILLEGSQLDASDWLNPAQVVLFSQQNSSGPWAMDLCARRLLDPCEHQCDPETGRREHRAAGECLCYEGYMKDPVHKHLCIRNEWGTNQGPWPYTIFQRGFDLVLGEQPSDKIFRFTYTLGEGMWLPLSKSFVIPPAELAINPSAKCKTDMTVMEDAVEVREELMTSSSFDSLEVLLDSFGPVRDCSKDNGGCSKNFRCISDRKLDSTGCVCPSGLSPMKDSSGCYDRHIGVDCSDGFNGGCEQLCLQQMAPFPDDPTLYNILMFCGCIEDYKLGVDGRSCQLITETCPEGSDCGESRELPMNQTLFGEMFFGYNNHSKEVAAGQVLKGTFRQNNFARGLDQQLPDGLVVATVPLENQCLEEISEPTPDPDFLTGMVNFSEVSGYPVLQHWKVRSVMYHIKLNQVAISQALSNALHSLDGATSRADFVALLDQFGNHYIQEAIYGFEESCSIWYPNKQVQRRLWLEYEDISKGNSPSDESEERERDPKVLTFPEYITSLSDSGTKHMAAGVRMECHSKGRCPSSCPLCHVTSSPDTPAEPVLLEVTKAAPIYELVTNNQTQRLLQEATMSSLWCSGTGDVIEDWCRCDSTAFGADGLPTCAPLPQPVLRLSTVHEPSSTLVVLEWEHSEPPIGVQIVDYLLRQEKVTDRMDHSKVETETVLSFVDDIISGAKSPCAMPSQVPDKQLTTISLIIRCLEPDTIYMFTLWGVDNTGRRSRPSDVIVKTPCPVVDDVKAQEIADKIYNLFNGYTSGKEQQTAYNTLLDLGSPTLHRVLYHYNQHYESFGEFTWRCEDELGPRLPACWDSQRSCR

Target ORF information:

RefSeq Version	XM_017001341.1
Organism	Homo sapiens(human)
Definition	Homo sapiens astrotactin 1 (ASTN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017001341.1

ORF Insert Sequence:

ATGGCTTTAG CCGGGCTCTG CGCCCTGCTC GCCTGCTGCT GGGGGCCGGC GGCGGTGCTG 
GCCACGGCCG CCGGCGACGT GGATCCATCC AAGGAGCTGG AGTGCAAGCT CAAAAGCATC 
ACGGTGTCGG CACTGCCCTT CCTGCGCGAG AACGACCTGA GCATCATGCA CAGCCCCTCG 
GCCTCGGAGC CCAAGCTCCT CTTCTCGGTG CGCAACGACT TCCCGGGAGA AATGGTCGTG 
GTGGACGACC TGGAGAACAC GGAGCTGCCC TACTTCGTGC TGGAGATCTC AGGGAACACA 
GAGGATATCC CTTTGGTGCG CTGGAGGCAG CAGTGGCTGG AGAATGGCAC TTTGCTTTTT 
CACATTCATC ACCAAGATGG TGCCCCAAGC CTTCCTGGAC AAGACCCCAC TGAAGAACCC 
CAACATGAGT CGGCAGAAGA GGAGCTGAGG ATCCTCCACA TCTCAGTCAT GGGTGGCATG 
ATCGCTCTGC TGCTGTCCAT CTTGTGCCTG GTGATGATCC TGTATACTCG CCGGCGCTGG 
TGCAAACGCC GCCGGGTCCC GCAGCCCCAG AAGAGTGCCA GTGCTGAGGC AGCCAATGAG 
ATTCACTACA TTCCTTCTGT GCTGATCGGC GGGCACGGAC GGGAGAGCCT GCGCAATGCC 
CGCGTGCAGG GCCACAACTC CAGTGGCACC CTGAGCATCC GGGAGACACC TATCCTGGAC 
GGCTATGAGT ATGACATCAC TGATCTGCGC CACCATCTGC AGAGGGAGTG CATGAACGGA 
GGGGAGGACT TTGCCAGCCA GGTCACGCGC ACCCTCGACT CCCTGCAGGG CTGCAATGAA 
AAGTCGGGGA TGGACCTCAC ACCAGGAAGT GACAATGCCA AGCTGTCACT GATGAACAAG 
TATAAAGATA ATATTATAGC CACTAGCCCT GTGGACTCCA ACCACCAGCA AGCCACCCTT 
CTCTCTCACA CCTCCAGCAG CCAGAGAAAG CGGATCAACA ACAAAGCAAG AGCTGGTTCC 
GCCTTCTTGA ACCCTGAAGG GGATTCTGGC ACAGAGGCAG AAAACGACCC CCAGCTGACC 
TTTTACACGG ATCCTTCAAG GAGCAGGAGG CGTAGTAGAG TGGGTTCTCC CCGAAGTCCT 
GTGAATAAGA CCACCTTGAC CCTGATCAGC ATCACCAGCT GTGTGATTGG CCTCGTGTGC 
TCCTCTCACG TCAACTGCCC TCTCGTTGTC AAGATCACCC TGCATGTCCC TGAGCACCTG 
ATTGCTGATG GGAGCCGCTT CATCTTGCTG GAGGGGAGCC AGCTGGATGC CAGTGACTGG 
CTGAACCCTG CCCAAGTGGT TCTCTTCTCT CAGCAGAACT CCAGCGGACC CTGGGCCATG 
GACCTCTGTG CCCGGCGGCT CCTGGACCCC TGTGAACACC AATGTGACCC CGAAACTGGT 
AGGCGGGAGC ACCGGGCAGC GGGGGAATGC CTCTGCTATG AAGGCTACAT GAAGGATCCA 
GTACATAAGC ACCTTTGCAT TCGGAACGAA TGGGGGACAA ACCAGGGGCC ATGGCCTTAC 
ACAATATTTC AGCGAGGCTT TGACCTGGTT TTGGGAGAGC AGCCCTCTGA TAAAATATTT 
AGATTCACCT ACACTCTTGG GGAGGGCATG TGGTTGCCCC TCAGCAAGAG CTTTGTGATT 
CCACCAGCCG AACTGGCCAT CAATCCATCA GCAAAGTGCA AGACGGACAT GACTGTGATG 
GAGGATGCTG TGGAGGTCAG AGAGGAGCTG ATGACTTCAT CCTCCTTCGA CAGCCTGGAG 
GTTCTCTTAG ATTCCTTTGG GCCGGTGCGC GACTGCAGCA AAGATAACGG GGGCTGCAGT 
AAGAATTTCC GCTGTATTTC AGATCGCAAG CTGGACTCCA CTGGTTGCGT GTGCCCATCT 
GGACTCAGTC CCATGAAGGA CAGCTCTGGC TGCTATGACC GCCACATCGG GGTGGACTGT 
TCCGACGGCT TCAACGGCGG CTGTGAGCAG CTGTGCCTCC AGCAGATGGC GCCCTTCCCG 
GACGACCCCA CCTTGTATAA CATCCTCATG TTCTGTGGGT GCATCGAGGA CTACAAGCTT 
GGTGTGGATG GACGCTCTTG CCAACTCATC ACGGAGACCT GTCCAGAGGG AAGTGACTGT 
GGGGAAAGCA GGGAGCTTCC CATGAACCAG ACCCTCTTTG GGGAGATGTT CTTTGGTTAC 
AACAACCATT CCAAGGAAGT GGCTGCCGGA CAGGTGCTGA AAGGAACATT CAGGCAAAAC 
AACTTTGCTC GTGGTTTAGA CCAGCAACTG CCAGATGGTC TTGTGGTGGC CACTGTGCCC 
CTGGAGAATC AATGCCTAGA GGAGATCTCG GAGCCCACCC CTGACCCTGA CTTCCTGACT 
GGGATGGTGA ACTTCAGTGA AGTGTCTGGG TACCCTGTGC TGCAGCACTG GAAGGTCCGG 
TCTGTGATGT ACCACATCAA ACTCAACCAA GTGGCCATCT CTCAGGCCCT CAGCAATGCT 
CTCCACTCGC TGGATGGGGC TACATCTCGT GCAGATTTTG TGGCGCTGTT GGACCAGTTC 
GGCAACCATT ACATCCAGGA AGCTATCTAC GGCTTTGAGG AGTCCTGTTC TATCTGGTAC 
CCAAACAAGC AGGTCCAGCG GCGACTCTGG CTGGAGTATG AAGACATCAG TAAAGGCAAC 
TCCCCATCAG ATGAGTCTGA GGAGCGGGAA AGAGACCCCA AGGTGCTGAC ATTCCCAGAA 
TACATCACCA GCTTGTCAGA CTCCGGCACC AAGCACATGG CGGCTGGAGT CCGCATGGAG 
TGCCACAGCA AGGGACGATG CCCCTCGTCC TGCCCCCTGT GTCATGTGAC ATCCAGCCCT 
GACACCCCTG CTGAGCCGGT TCTGCTGGAG GTGACCAAAG CAGCCCCCAT CTATGAACTA 
GTGACCAACA ACCAGACCCA GAGGCTCTTG CAGGAGGCTA CCATGAGCTC TCTCTGGTGC 
TCAGGGACTG GAGATGTCAT CGAGGACTGG TGTCGATGTG ACTCCACTGC TTTTGGAGCT 
GATGGACTCC CCACCTGTGC GCCTCTCCCA CAGCCTGTGC TGAGACTCTC CACGGTTCAC 
GAGCCCAGCA GCACTCTTGT GGTCCTGGAG TGGGAACACT CAGAGCCACC AATCGGGGTG 
CAGATTGTAG ATTACCTCCT CCGTCAAGAG AAAGTCACTG ACAGGATGGA CCACTCCAAA 
GTGGAGACAG AAACAGTGCT GAGCTTTGTG GACGACATCA TCTCTGGAGC AAAGTCTCCT 
TGTGCAATGC CATCTCAGGT GCCGGACAAG CAGCTCACCA CCATCTCTCT GATCATACGA 
TGCCTGGAAC CTGACACCAT TTACATGTTC ACGCTGTGGG GAGTGGACAA CACAGGACGG 
CGCTCCAGGC CAAGCGACGT GATCGTGAAG ACCCCATGCC CCGTGGTGGA TGATGTCAAG 
GCTCAAGAAA TAGCAGACAA GATCTACAAT CTCTTCAATG GCTACACTAG TGGGAAGGAG 
CAGCAGACCG CCTACAACAC CCTCCTGGAT CTGGGTTCCC CCACCTTACA CCGGGTCCTC 
TACCACTATA ACCAGCACTA TGAGAGTTTT GGGGAATTCA CCTGGCGATG TGAGGATGAG 
TTAGGTCCCA GGCTTCCAGC GTGTTGGGAC AGTCAGAGGA GTTGTAGATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ASTN1 and alcohol dependence: family-based association analysis in multiplex alcohol dependence families.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics159B(4)445-55(2012 Jun)
Hill SY,Weeks DE,Jones BL,Zezza N,Stiffler S

Identification of new putative susceptibility genes for several psychiatric disorders by association analysis of regulatory and non-synonymous SNPs of 306 genes involved in neurotransmission and neurodevelopment.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics150B(6)808-16(2009 Sep)
Gratac?s M,Costas J,de Cid R,Bay?s M,Gonz?lez JR,Baca-Garc?a E,de Diego Y,Fern?ndez-Aranda F,Fern?ndez-Piqueras J,Guitart M,Mart?n-Santos R,Martorell L,Mench?n JM,Roca M,S?iz-Ruiz J,Sanju?n J,Torrens M,Urretavizcaya M,Valero J,Vilella E,Estivill X,Carracedo A,

Association analysis of schizophrenia on 18 genes involved in neuronal migration: MDGA1 as a new susceptibility gene.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics147B(7)1089-100(2008 Oct)
K?hler AK,Djurovic S,Kulle B,J?nsson EG,Agartz I,Hall H,Opjordsmoen S,Jakobsen KD,Hansen T,Melle I,Werge T,Steen VM,Andreassen OA

Molecular genetics of successful smoking cessation: convergent genome-wide association study results.
Archives of general psychiatry65(6)683-93(2008 Jun)
Uhl GR,Liu QR,Drgon T,Johnson C,Walther D,Rose JE,David SP,Niaura R,Lerman C

Construction and characterization of human brain cDNA libraries suitable for analysis of cDNA clones encoding relatively large proteins.
DNA research : an international journal for rapid publication of reports on genes and genomes4(1)53-9(1997 Feb)
Ohara O,Nagase T,Ishikawa K,Nakajima D,Ohira M,Seki N,Nomura N

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ASTN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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