MMUT cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MMUT
Entrez Gene ID	4594
Full Name	methylmalonyl-CoA mutase
Synonyms	MCM
General protein information	Preferred Names methylmalonyl-CoA mutase Names methylmalonyl-CoA mutase, mitochondrial methylmalonyl Coenzyme A mutase methylmalonyl-CoA isomerase mutant methylmalonyl CoA mutase truncated methylmalonyl CoA mutase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6p12.3
Summary	This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008].
Disorder MIM:	609058
Disorder Html:	Methylmalonic aciduria, mut(0) type, 251000 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000255.3	NP_000246.2	methylmalonyl-CoA mutase, mitochondrial precursor
NM_000255.4	NP_000246.2	methylmalonyl-CoA mutase, mitochondrial precursor
XM_005249143.3	XP_005249200.1	methylmalonyl-CoA mutase, mitochondrial isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Mut	XP_006244706.1
Gallus gallus (chicken)	MUT	XP_004935974.1
Danio rerio (zebrafish)	mut	NP_001092696.1
Caenorhabditis elegans (roundworm)	mmcm-1	NP_497786.2
Mus musculus (house mouse)	Mut	NP_032676.2
Canis lupus familiaris (dog)	MUT	XP_532164.3
Pan troglodytes (chimpanzee)	MUT	XP_001146876.1
	MUT	XP_001104531.1
Homo sapiens (human)	MUT	NP_000246.2
Bos taurus (cattle)	MUT	NP_776364.2
Xenopus tropicalis (tropical clawed frog)	mut	XP_004915010.1

Gene Ontology
Bibliography

Related articles in PubMed

TAT-MTS-MCM fusion proteins reduce MMA levels and improve mitochondrial activity and liver function in MCM-deficient cells.
Erlich-Hadad T, Hadad R, Feldman A, Greif H, Lictenstein M, Lorberboum-Galski H
Journal of cellular and molecular medicine(2017 Dec)

Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria.
Plessl T, B?rer C, Lutz S, Yue WW, Baumgartner MR, Froese DS
Human mutation38(8)988-1001(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we identified seven novel genetic variants: p.Leu549Pro, p.Glu564*, p.Leu641Pro in MUT, p.Tyr206Cys in PCCB, p.His194Arg, p.Val298Met in BCKDHA and p.Glu286_Met290del in BCKDHB gene. In silico and/or eukaryotic expression studies confirmed pathogenic effect of all novel genetic variants
Title: Molecular and phenotypic characteristics of seven novel mutations causing branched-chain organic acidurias.

A total of 54 different mutations in MUT were identified in 48 patients; 16 novel mutations were identified... In five patients, the NGS panel did not confirm the diagnosis made by complementation analysis. One of these patients was found to carry 2 novel mutations
Title: Next generation sequencing of patients with mut methylmalonic aciduria: Validation of somatic cell studies and identification of 16 novel mutations.

Study identified 41 novel mutations in patients with methylmalonic aciduria (MMA); most of them were missense mutations. The absence of MUT protein in most of the patient cell lines, suggesting protein instability as a major mechanism of deficiency in mut-type MMA.
Title: Molecular Genetic Characterization of 151 Mut-Type Methylmalonic Aciduria Patients and Identification of 41 Novel Mutations in MUT.

Two novel mutations of the MUT gene, including c.581C>T (p.P194L) and c.1219A>T (p.N407Y), are associated with methylmalonic academia in a Chinese family.
Title: [Identification of two novel mutations of MUT gene in a Chinese family affected with isolated methylmalonic acidemia].

In methylmalonic acidemia,a total of 10 novel MUT mutations were detected in the Chinese population. c.729_730insTT (p.D244Lfs*39) was the most frequent mutation.
Title: Clinical features and MUT gene mutation spectrum in Chinese patients with isolated methylmalonic acidemia: identification of ten novel allelic variants.

The following MMUT gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMUT cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25184	NM_000255.4 Latest version!	Homo sapiens methylmalonyl-CoA mutase (MMUT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25184	XM_005249143.3 Latest version!	Homo sapiens methylmalonyl-CoA mutase (MMUT), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
View Old Accession Versions >>
OHu25184	NM_000255.3	Homo sapiens methylmalonyl-CoA mutase (MUT), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MMUT ( NM_000255.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000246.2 Homo sapiens methylmalonyl-CoA mutase (MUT), mRNA.

Price & Availability↑

CloneID	OHu25184
Clone ID Related Accession (Same CDS sequence)	NM_000255.3 , NM_000255.4 , XM_005249143.3
Accession Version	NM_000255.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	methylmalonyl-CoA mutase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA777441.1, AK292568.1, AK312611.1 and AL590668.5. This sequence is a reference standard in the RefSeqGene project. On May 13, 2010 this sequence version replaced NM_000255.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.51261.1, SRR1803614.150698.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC 
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA 
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC 
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT 
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT 
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG 
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT 
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA 
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA 
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT 
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA 
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA 
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT 
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA 
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA 
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA 
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG 
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA 
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA 
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA 
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT 
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT 
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT 
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA 
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG 
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT 
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT 
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT 
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT 
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA 
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT 
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG 
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC 
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG 
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT 
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG 
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000246.2
CDS	266..2518
Translation	MLRAKNQLFLLSPHYLRQVKESSGSRLIQQRLLHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPEGISIKPLYSKRDTMDLPEELPGVKPFTRGPYPTMYTFRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPVLANFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMKIIADIFEYTAKHMPKFNSISISGYHMQEAGADAILELAYTLADGLEYSRTGLQAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTSGWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV

Target ORF information:

RefSeq Version	NM_000255.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methylmalonyl-CoA mutase (MUT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000255.3

ORF Insert Sequence:

ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC 
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA 
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC 
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT 
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT 
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG 
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT 
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA 
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA 
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT 
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA 
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA 
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT 
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA 
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA 
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA 
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG 
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA 
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA 
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA 
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT 
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT 
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT 
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA 
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG 
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT 
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT 
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT 
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT 
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA 
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT 
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG 
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC 
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG 
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT 
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG 
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMUT ( NM_000255.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000246.2 Homo sapiens methylmalonyl-CoA mutase (MMUT), mRNA.

Price & Availability↑

CloneID	OHu25184
Clone ID Related Accession (Same CDS sequence)	NM_000255.3 , NM_000255.4 , XM_005249143.3
Accession Version	NM_000255.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	methylmalonyl-CoA mutase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK292568.1, AK312611.1 and AL590668.5. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000255.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: MF170176.1, MF170177.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000274813.4/ ENSP00000274813.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC 
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA 
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC 
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT 
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT 
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG 
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT 
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA 
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA 
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT 
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA 
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA 
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT 
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA 
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA 
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA 
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG 
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA 
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA 
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA 
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT 
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT 
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT 
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA 
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG 
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT 
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT 
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT 
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT 
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA 
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT 
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG 
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC 
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG 
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT 
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG 
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000246.2
CDS	191..2443
Translation	MLRAKNQLFLLSPHYLRQVKESSGSRLIQQRLLHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPEGISIKPLYSKRDTMDLPEELPGVKPFTRGPYPTMYTFRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPVLANFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMKIIADIFEYTAKHMPKFNSISISGYHMQEAGADAILELAYTLADGLEYSRTGLQAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTSGWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV

Target ORF information:

RefSeq Version	NM_000255.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methylmalonyl-CoA mutase (MMUT), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000255.4

ORF Insert Sequence:

ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC 
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA 
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC 
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT 
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT 
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG 
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT 
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA 
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA 
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT 
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA 
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA 
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT 
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA 
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA 
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA 
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG 
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA 
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA 
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA 
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT 
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT 
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT 
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA 
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG 
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT 
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT 
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT 
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT 
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA 
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT 
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG 
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC 
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG 
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT 
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG 
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMUT ( XM_005249143.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005249200.1 Homo sapiens methylmalonyl-CoA mutase (MMUT), transcript variant X1, mRNA.

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CloneID	OHu25184
Clone ID Related Accession (Same CDS sequence)	NM_000255.3 , NM_000255.4 , XM_005249143.3
Accession Version	XM_005249143.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2253bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	methylmalonyl-CoA mutase, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005249143.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC 
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA 
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC 
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT 
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT 
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG 
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT 
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA 
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA 
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT 
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA 
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA 
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT 
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA 
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA 
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA 
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG 
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA 
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA 
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA 
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT 
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT 
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT 
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA 
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG 
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT 
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT 
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT 
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT 
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA 
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT 
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG 
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC 
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG 
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT 
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG 
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005249200.1
CDS	171..2423
Translation	MLRAKNQLFLLSPHYLRQVKESSGSRLIQQRLLHQQQPLHPEWAALAKKQLKGKNPEDLIWHTPEGISIKPLYSKRDTMDLPEELPGVKPFTRGPYPTMYTFRPWTIRQYAGFSTVEESNKFYKDNIKAGQQGLSVAFDLATHRGYDSDNPRVRGDVGMAGVAIDTVEDTKILFDGIPLEKMSVSMTMNGAVIPVLANFIVTGEEQGVPKEKLTGTIQNDILKEFMVRNTYIFPPEPSMKIIADIFEYTAKHMPKFNSISISGYHMQEAGADAILELAYTLADGLEYSRTGLQAGLTIDEFAPRLSFFWGIGMNFYMEIAKMRAGRRLWAHLIEKMFQPKNSKSLLLRAHCQTSGWSLTEQDPYNNIVRTAIEAMAAVFGGTQSLHTNSFDEALGLPTVKSARIARNTQIIIQEESGIPKVADPWGGSYMMECLTNDVYDAALKLINEIEEMGGMAKAVAEGIPKLRIEECAARRQARIDSGSEVIVGVNKYQLEKEDAVEVLAIDNTSVRNRQIEKLKKIKSSRDQALAERCLAALTECAASGDGNILALAVDASRARCTVGEITDALKKVFGEHKANDRMVSGAYRQEFGESKEITSAIKRVHKFMEREGRRPRLLVAKMGQDGHDRGAKVIATGFADLGFDVDIGPLFQTPREVAQQAVDADVHAVGISTLAAGHKTLVPELIKELNSLGRPDILVMCGGVIPPQDYEFLFEVGVSNVFGPGTRIPKAAVQVLDDIEKCLEKKQQSV

Target ORF information:

RefSeq Version	XM_005249143.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methylmalonyl-CoA mutase (MMUT), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005249143.3

ORF Insert Sequence:

ATGTTAAGAG CTAAGAATCA GCTTTTTTTA CTTTCACCTC ATTACCTGAG GCAGGTAAAA 
GAATCATCAG GCTCCAGGCT CATACAGCAA CGACTTCTAC ACCAGCAACA GCCCCTTCAC 
CCAGAATGGG CTGCCCTGGC TAAAAAGCAG CTGAAAGGCA AAAACCCAGA AGACCTAATA 
TGGCACACCC CGGAAGGGAT CTCTATAAAA CCCTTGTATT CCAAGAGAGA TACTATGGAC 
TTACCTGAAG AACTTCCAGG AGTGAAGCCA TTCACACGTG GACCATATCC TACCATGTAT 
ACCTTTAGGC CCTGGACCAT CCGCCAGTAT GCTGGTTTTA GTACTGTGGA AGAAAGCAAT 
AAGTTCTATA AGGACAACAT TAAGGCTGGT CAGCAGGGAT TATCAGTTGC CTTTGATCTG 
GCGACACATC GTGGCTATGA TTCAGACAAC CCTCGAGTTC GTGGTGATGT TGGAATGGCT 
GGAGTTGCTA TTGACACTGT GGAAGATACC AAAATTCTTT TTGATGGAAT TCCTTTAGAA 
AAAATGTCAG TTTCCATGAC TATGAATGGA GCAGTTATTC CAGTTCTTGC AAATTTTATA 
GTAACTGGAG AAGAACAAGG TGTACCTAAA GAGAAGCTTA CTGGTACCAT CCAAAATGAT 
ATACTAAAGG AATTTATGGT TCGAAATACA TACATTTTTC CTCCAGAACC ATCCATGAAA 
ATTATTGCTG ACATATTTGA ATATACAGCA AAGCACATGC CAAAATTTAA TTCAATTTCA 
ATTAGTGGAT ACCATATGCA GGAAGCAGGG GCTGATGCCA TTCTGGAGCT GGCCTATACT 
TTAGCAGATG GATTGGAGTA CTCTAGAACT GGACTCCAGG CTGGCCTGAC AATTGATGAA 
TTTGCACCAA GGTTGTCTTT CTTCTGGGGA ATTGGAATGA ATTTCTATAT GGAAATAGCA 
AAGATGAGAG CTGGTAGAAG ACTCTGGGCT CACTTAATAG AGAAAATGTT TCAGCCTAAA 
AACTCAAAAT CTCTTCTTCT AAGAGCACAC TGTCAGACAT CTGGATGGTC ACTTACTGAG 
CAGGATCCCT ACAATAATAT TGTCCGTACT GCAATAGAAG CAATGGCAGC AGTATTTGGA 
GGGACTCAGT CTTTGCACAC AAATTCTTTT GATGAAGCTT TGGGTTTGCC AACTGTGAAA 
AGTGCTCGAA TTGCCAGGAA CACACAAATC ATCATTCAAG AAGAATCTGG GATTCCCAAA 
GTGGCTGATC CTTGGGGAGG TTCTTACATG ATGGAATGTC TCACAAATGA TGTTTATGAT 
GCTGCTTTAA AGCTCATTAA TGAAATTGAA GAAATGGGTG GAATGGCCAA AGCTGTAGCT 
GAGGGAATAC CTAAACTTCG AATTGAAGAA TGTGCTGCCC GAAGACAAGC TAGAATAGAT 
TCTGGTTCTG AAGTAATTGT TGGAGTAAAT AAGTACCAGT TGGAAAAAGA AGACGCTGTA 
GAAGTTCTGG CAATTGATAA TACTTCAGTG CGAAACAGGC AGATTGAAAA ACTTAAGAAG 
ATCAAATCCA GCAGGGATCA AGCTTTGGCT GAACGTTGTC TTGCTGCACT AACCGAATGT 
GCTGCTAGCG GAGATGGAAA TATCCTGGCT CTTGCAGTGG ATGCATCTCG GGCAAGATGT 
ACAGTGGGAG AAATCACAGA TGCCCTGAAA AAGGTATTTG GTGAACATAA AGCGAATGAT 
CGAATGGTGA GTGGAGCATA TCGCCAGGAA TTTGGAGAAA GTAAAGAGAT AACATCTGCT 
ATCAAGAGGG TTCATAAATT CATGGAACGT GAAGGTCGCA GACCTCGTCT TCTTGTAGCA 
AAAATGGGAC AAGATGGCCA TGACAGAGGA GCAAAAGTTA TTGCTACAGG ATTTGCTGAT 
CTTGGTTTTG ATGTGGACAT AGGCCCTCTT TTCCAGACTC CTCGTGAAGT GGCCCAGCAG 
GCTGTGGATG CGGATGTGCA TGCTGTGGGC ATAAGCACCC TCGCTGCTGG TCATAAAACC 
CTAGTTCCTG AACTCATCAA AGAACTTAAC TCCCTTGGAC GGCCAGATAT TCTTGTCATG 
TGTGGAGGGG TGATACCACC TCAGGATTAT GAATTTCTGT TTGAAGTTGG TGTTTCCAAT 
GTATTTGGTC CTGGGACTCG AATTCCAAAG GCTGCCGTTC AGGTGCTTGA TGATATTGAG 
AAGTGTTTGG AAAAGAAGCA GCAATCTGTA TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

TAT-MTS-MCM fusion proteins reduce MMA levels and improve mitochondrial activity and liver function in MCM-deficient cells.
Journal of cellular and molecular medicine(2017 Dec)
Erlich-Hadad T,Hadad R,Feldman A,Greif H,Lictenstein M,Lorberboum-Galski H

Protein destabilization and loss of protein-protein interaction are fundamental mechanisms in cblA-type methylmalonic aciduria.
Human mutation38(8)988-1001(2017 Aug)
Plessl T,B?rer C,Lutz S,Yue WW,Baumgartner MR,Froese DS

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MMUT cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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