MTAP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTAP
Entrez Gene ID	4507
Full Name	methylthioadenosine phosphorylase
Synonyms	BDMF,DMSFH,DMSMFH,HEL-249,LGMBF,MSAP,c86fus
General protein information	Preferred Names methylthioadenosine phosphorylase Names S-methyl-5'-thioadenosine phosphorylase 5'-methylthioadenosine phosphorylase MTA phosphorylase MTAPase MeSAdo phosphorylase epididymis luminal protein 249
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9p21.3
Summary	This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown. [provided by RefSeq, Jul 2008].
Disorder MIM:	156540
Disorder Html:	Diaphyseal medullary stenosis with malignant fibrous histiocytoma, 112250 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002451.3	NP_002442.2	S-methyl-5'-thioadenosine phosphorylase
NM_002451.4	NP_002442.2	S-methyl-5'-thioadenosine phosphorylase

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MTAP	XP_531959.3
Drosophila melanogaster (fruit fly)	CG4802	NP_001261049.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005129	XP_314011.4
Magnaporthe oryzae (rice blast fungus)	MGG_06498	XP_003717062.1
Xenopus tropicalis (tropical clawed frog)	mtap	NP_001011495.1
	MTAP	XP_001107077.1
Bos taurus (cattle)	MTAP	XP_002689552.1
Caenorhabditis elegans (roundworm)	B0228.7	NP_495629.2
Homo sapiens (human)	MTAP	NP_002442.2
Pan troglodytes (chimpanzee)	MTAP	XP_001153022.1
Mus musculus (house mouse)	Mtap	NP_077753.1
Kluyveromyces lactis	KLLA0F15664g	XP_455784.1
Gallus gallus (chicken)	MTAP	NP_001026775.2
Danio rerio (zebrafish)	mtap	NP_956848.1
Saccharomyces cerevisiae (baker's yeast)	MEU1	NP_013117.1
Schizosaccharomyces pombe (fission yeast)	SPAC16C9.02c	NP_593076.1
Neurospora crassa	NCU03963	XP_957602.1

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of methylthioadenosin phosphorylase (MTAP) expression in colorectal cancer.
Zhong Y, Lu K, Zhu S, Li W, Sun S
Artificial cells, nanomedicine, and biotechnology1-6(2017 Dec)

Melanoma risk alleles are associated with downregulation of the MTAP gene and hypermethylation of a CpG island upstream of the gene in dermal fibroblasts.
Sangalli A, Malerba G, Tessari G, Rodolfo M, Gomez-Lira M
Experimental dermatology26(8)733-736(2017 Aug)

The research on association of copy number variation in chromosome 9p21 region with atherothrombotic stroke in the Han Chinese population.
Liu H, Yang M, Wang X, Ji Y, Zhao J, Liu W, Zheng J
Journal of the neurological sciences37788-94(2017 Jun)

MTAP Deletions in Cancer Create Vulnerability to Targeting of the MAT2A/PRMT5/RIOK1 Axis.
Marjon K, Cameron MJ, Quang P, Clasquin MF, Mandley E, Kunii K, McVay M, Choe S, Kernytsky A, Gross S, Konteatis Z, Murtie J, Blake ML, Travins J, Dorsch M, Biller SA, Marks KM
Cell reports15(3)574-587(2016 Apr)

Characterization of the Methylthioadenosine Phosphorylase Polymorphism rs7023954 - Incidence and Effects on Enzymatic Function in Malignant Melanoma.
Limm K, Dettmer K, Reinders J, Oefner PJ, Bosserhoff AK
PloS one11(8)e0160348(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data demonstrate that MTAP deficiency leads to accumulation of MTA inducing deregulation of central cellular pathways that control cell fate and contributes to the onset of a proliferative and antiapoptotic phenotype in the liver.
Title: Methylthioadenosine (MTA) Regulates Liver Cells Proteome and Methylproteome: Implications in Liver Biology and Disease.

methionine adenosyltransferase II alpha (MAT2A), and the arginine methyltransferase, PRMT5, as vulnerable enzymes in cells with MTAP deletion.
Title: MTAP Deletions in Cancer Create Vulnerability to Targeting of the MAT2A/PRMT5/RIOK1 Axis.

High MTAP expression is associated with prostate cancer.
Title: The essential role of methylthioadenosine phosphorylase in prostate cancer.

Results indicate complex rearrangements involving CDKN2A locus and frequent occurrence of fusion transcripts involving methylthioadenosine phosphorylase (MTAP) and ANRIL genes.
Title: Mapping of deletion breakpoints at the CDKN2A locus in melanoma: detection of MTAP-ANRIL fusion transcripts.

The SNP rs7023954 of methylthioadenosine phosphorylase co-dominantly expressed alleles revealed no difference in the conversion rate of 5'-methylthioadenosine to adenine and 5-methylthioribose-1-phosphate, indicating that this known enzymatic activity does not modulate the tumor suppressive function of methylthioadenosine phosphorylase.
Title: Characterization of the Methylthioadenosine Phosphorylase Polymorphism rs7023954 - Incidence and Effects on Enzymatic Function in Malignant Melanoma.

The following MTAP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTAP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19116	NM_002451.4 Latest version!	Homo sapiens methylthioadenosine phosphorylase (MTAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu19116	NM_002451.3	Homo sapiens methylthioadenosine phosphorylase (MTAP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTAP ( NM_002451.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002442.2 Homo sapiens methylthioadenosine phosphorylase (MTAP), mRNA.

Price & Availability↑

CloneID	OHu19116
Clone ID Related Accession (Same CDS sequence)	NM_002451.3 , NM_002451.4
Accession Version	NM_002451.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	S-methyl-5'-thioadenosine phosphorylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U22233.1, L40432.1, BC026106.1, BM994282.1, BQ220538.1, BQ232183.1, BG576334.1, AK024734.1 and CA440131.1. This sequence is a reference standard in the RefSeqGene project. On May 12, 2004 this sequence version replaced NM_002451.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.213756.1, SRR1660807.69641.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCTG GCACCACCAC CACCGCCGTG AAGATTGGAA TAATTGGTGG AACAGGCCTG 
GATGATCCAG AAATTTTAGA AGGAAGAACT GAAAAATATG TGGATACTCC ATTTGGCAAG 
CCATCTGATG CCTTAATTTT GGGGAAGATA AAAAATGTTG ATTGCGTCCT CCTTGCAAGG 
CATGGAAGGC AGCACACCAT CATGCCTTCA AAGGTCAACT ACCAGGCGAA CATCTGGGCT 
TTGAAGGAAG AGGGCTGTAC ACATGTCATA GTGACCACAG CTTGTGGCTC CTTGAGGGAG 
GAGATTCAGC CCGGCGATAT TGTCATTATT GATCAGTTCA TTGACAGGAC CACTATGAGA 
CCTCAGTCCT TCTATGATGG AAGTCATTCT TGTGCCAGAG GAGTGTGCCA TATTCCAATG 
GCTGAGCCGT TTTGCCCCAA AACGAGAGAG GTTCTTATAG AGACTGCTAA GAAGCTAGGA 
CTCCGGTGCC ACTCAAAGGG GACAATGGTC ACAATCGAGG GACCTCGTTT TAGCTCCCGG 
GCAGAAAGCT TCATGTTCCG CACCTGGGGG GCGGATGTTA TCAACATGAC CACAGTTCCA 
GAGGTGGTTC TTGCTAAGGA GGCTGGAATT TGTTACGCAA GTATCGCCAT GGCGACAGAT 
TATGACTGCT GGAAGGAGCA CGAGGAAGCA GTTTCGGTGG ACCGGGTCTT AAAGACCCTG 
AAAGAAAACG CTAATAAAGC CAAAAGCTTA CTGCTCACTA CCATACCTCA GATAGGGTCC 
ACAGAATGGT CAGAAACCCT CCATAACCTG AAGAATATGG CCCAGTTTTC TGTTTTATTA 
CCAAGACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002442.2
CDS	114..965
Translation	MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCVLLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDRTTMRPQSFYDGSHSCARGVCHIPMAEPFCPKTREVLIETAKKLGLRCHSKGTMVTIEGPRFSSRAESFMFRTWGADVINMTTVPEVVLAKEAGICYASIAMATDYDCWKEHEEAVSVDRVLKTLKENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQFSVLLPRH

Target ORF information:

RefSeq Version	NM_002451.3
Organism	Homo sapiens(human)
Definition	Homo sapiens methylthioadenosine phosphorylase (MTAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002451.3

ORF Insert Sequence:

ATGGCCTCTG GCACCACCAC CACCGCCGTG AAGATTGGAA TAATTGGTGG AACAGGCCTG 
GATGATCCAG AAATTTTAGA AGGAAGAACT GAAAAATATG TGGATACTCC ATTTGGCAAG 
CCATCTGATG CCTTAATTTT GGGGAAGATA AAAAATGTTG ATTGCGTCCT CCTTGCAAGG 
CATGGAAGGC AGCACACCAT CATGCCTTCA AAGGTCAACT ACCAGGCGAA CATCTGGGCT 
TTGAAGGAAG AGGGCTGTAC ACATGTCATA GTGACCACAG CTTGTGGCTC CTTGAGGGAG 
GAGATTCAGC CCGGCGATAT TGTCATTATT GATCAGTTCA TTGACAGGAC CACTATGAGA 
CCTCAGTCCT TCTATGATGG AAGTCATTCT TGTGCCAGAG GAGTGTGCCA TATTCCAATG 
GCTGAGCCGT TTTGCCCCAA AACGAGAGAG GTTCTTATAG AGACTGCTAA GAAGCTAGGA 
CTCCGGTGCC ACTCAAAGGG GACAATGGTC ACAATCGAGG GACCTCGTTT TAGCTCCCGG 
GCAGAAAGCT TCATGTTCCG CACCTGGGGG GCGGATGTTA TCAACATGAC CACAGTTCCA 
GAGGTGGTTC TTGCTAAGGA GGCTGGAATT TGTTACGCAA GTATCGCCAT GGCGACAGAT 
TATGACTGCT GGAAGGAGCA CGAGGAAGCA GTTTCGGTGG ACCGGGTCTT AAAGACCCTG 
AAAGAAAACG CTAATAAAGC CAAAAGCTTA CTGCTCACTA CCATACCTCA GATAGGGTCC 
ACAGAATGGT CAGAAACCCT CCATAACCTG AAGAATATGG CCCAGTTTTC TGTTTTATTA 
CCAAGACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTAP ( NM_002451.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002442.2 Homo sapiens methylthioadenosine phosphorylase (MTAP), mRNA.

Price & Availability↑

CloneID	OHu19116
Clone ID Related Accession (Same CDS sequence)	NM_002451.3 , NM_002451.4
Accession Version	NM_002451.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	S-methyl-5'-thioadenosine phosphorylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U22233.1, L40432.1, BC026106.1, BM994282.1, BQ220538.1, BQ232183.1, BG576334.1, AK024734.1, CA440131.1 and AL359922.10. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002451.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GQ891397.1, SRR1660805.213756.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000644715.2/ ENSP00000494373.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCTCTG GCACCACCAC CACCGCCGTG AAGATTGGAA TAATTGGTGG AACAGGCCTG 
GATGATCCAG AAATTTTAGA AGGAAGAACT GAAAAATATG TGGATACTCC ATTTGGCAAG 
CCATCTGATG CCTTAATTTT GGGGAAGATA AAAAATGTTG ATTGCGTCCT CCTTGCAAGG 
CATGGAAGGC AGCACACCAT CATGCCTTCA AAGGTCAACT ACCAGGCGAA CATCTGGGCT 
TTGAAGGAAG AGGGCTGTAC ACATGTCATA GTGACCACAG CTTGTGGCTC CTTGAGGGAG 
GAGATTCAGC CCGGCGATAT TGTCATTATT GATCAGTTCA TTGACAGGAC CACTATGAGA 
CCTCAGTCCT TCTATGATGG AAGTCATTCT TGTGCCAGAG GAGTGTGCCA TATTCCAATG 
GCTGAGCCGT TTTGCCCCAA AACGAGAGAG GTTCTTATAG AGACTGCTAA GAAGCTAGGA 
CTCCGGTGCC ACTCAAAGGG GACAATGGTC ACAATCGAGG GACCTCGTTT TAGCTCCCGG 
GCAGAAAGCT TCATGTTCCG CACCTGGGGG GCGGATGTTA TCAACATGAC CACAGTTCCA 
GAGGTGGTTC TTGCTAAGGA GGCTGGAATT TGTTACGCAA GTATCGCCAT GGCGACAGAT 
TATGACTGCT GGAAGGAGCA CGAGGAAGCA GTTTCGGTGG ACCGGGTCTT AAAGACCCTG 
AAAGAAAACG CTAATAAAGC CAAAAGCTTA CTGCTCACTA CCATACCTCA GATAGGGTCC 
ACAGAATGGT CAGAAACCCT CCATAACCTG AAGAATATGG CCCAGTTTTC TGTTTTATTA 
CCAAGACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002442.2
CDS	114..965
Translation	MASGTTTTAVKIGIIGGTGLDDPEILEGRTEKYVDTPFGKPSDALILGKIKNVDCVLLARHGRQHTIMPSKVNYQANIWALKEEGCTHVIVTTACGSLREEIQPGDIVIIDQFIDRTTMRPQSFYDGSHSCARGVCHIPMAEPFCPKTREVLIETAKKLGLRCHSKGTMVTIEGPRFSSRAESFMFRTWGADVINMTTVPEVVLAKEAGICYASIAMATDYDCWKEHEEAVSVDRVLKTLKENANKAKSLLLTTIPQIGSTEWSETLHNLKNMAQFSVLLPRH

Target ORF information:

RefSeq Version	NM_002451.4
Organism	Homo sapiens(human)
Definition	Homo sapiens methylthioadenosine phosphorylase (MTAP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002451.4

ORF Insert Sequence:

ATGGCCTCTG GCACCACCAC CACCGCCGTG AAGATTGGAA TAATTGGTGG AACAGGCCTG 
GATGATCCAG AAATTTTAGA AGGAAGAACT GAAAAATATG TGGATACTCC ATTTGGCAAG 
CCATCTGATG CCTTAATTTT GGGGAAGATA AAAAATGTTG ATTGCGTCCT CCTTGCAAGG 
CATGGAAGGC AGCACACCAT CATGCCTTCA AAGGTCAACT ACCAGGCGAA CATCTGGGCT 
TTGAAGGAAG AGGGCTGTAC ACATGTCATA GTGACCACAG CTTGTGGCTC CTTGAGGGAG 
GAGATTCAGC CCGGCGATAT TGTCATTATT GATCAGTTCA TTGACAGGAC CACTATGAGA 
CCTCAGTCCT TCTATGATGG AAGTCATTCT TGTGCCAGAG GAGTGTGCCA TATTCCAATG 
GCTGAGCCGT TTTGCCCCAA AACGAGAGAG GTTCTTATAG AGACTGCTAA GAAGCTAGGA 
CTCCGGTGCC ACTCAAAGGG GACAATGGTC ACAATCGAGG GACCTCGTTT TAGCTCCCGG 
GCAGAAAGCT TCATGTTCCG CACCTGGGGG GCGGATGTTA TCAACATGAC CACAGTTCCA 
GAGGTGGTTC TTGCTAAGGA GGCTGGAATT TGTTACGCAA GTATCGCCAT GGCGACAGAT 
TATGACTGCT GGAAGGAGCA CGAGGAAGCA GTTTCGGTGG ACCGGGTCTT AAAGACCCTG 
AAAGAAAACG CTAATAAAGC CAAAAGCTTA CTGCTCACTA CCATACCTCA GATAGGGTCC 
ACAGAATGGT CAGAAACCCT CCATAACCTG AAGAATATGG CCCAGTTTTC TGTTTTATTA 
CCAAGACATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of methylthioadenosin phosphorylase (MTAP) expression in colorectal cancer.
Artificial cells, nanomedicine, and biotechnology1-6(2017 Dec)
Zhong Y,Lu K,Zhu S,Li W,Sun S

Melanoma risk alleles are associated with downregulation of the MTAP gene and hypermethylation of a CpG island upstream of the gene in dermal fibroblasts.
Experimental dermatology26(8)733-736(2017 Aug)
Sangalli A,Malerba G,Tessari G,Rodolfo M,Gomez-Lira M

The research on association of copy number variation in chromosome 9p21 region with atherothrombotic stroke in the Han Chinese population.
Journal of the neurological sciences37788-94(2017 Jun)
Liu H,Yang M,Wang X,Ji Y,Zhao J,Liu W,Zheng J

MTAP Deletions in Cancer Create Vulnerability to Targeting of the MAT2A/PRMT5/RIOK1 Axis.
Cell reports15(3)574-587(2016 Apr)
Marjon K,Cameron MJ,Quang P,Clasquin MF,Mandley E,Kunii K,McVay M,Choe S,Kernytsky A,Gross S,Konteatis Z,Murtie J,Blake ML,Travins J,Dorsch M,Biller SA,Marks KM

Characterization of the Methylthioadenosine Phosphorylase Polymorphism rs7023954 - Incidence and Effects on Enzymatic Function in Malignant Melanoma.
PloS one11(8)e0160348(2016)
Limm K,Dettmer K,Reinders J,Oefner PJ,Bosserhoff AK

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MTAP cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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