MT3 cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MT3
Entrez Gene ID	4504
Full Name	metallothionein 3
Synonyms	GIF,GIFB,GRIF,ZnMT3
General protein information	Preferred Names metallothionein 3 Names metallothionein-3 growth inhibitory factor metallothionein 3 (growth inhibitory factor (neurotrophic)) metallothionein-III
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 16 Map Location: 16q13
Summary	This gene is a member of the metallothionein family of genes. Proteins encoded by this gene family are low in molecular weight, are cysteine-rich, lack aromatic residues, and bind divalent heavy metal ions. This gene family member displays tissue-specific expression, and contains a threonine insert near its N-terminus and a glutamate-rich hexapeptide insert near its C-terminus relative to the proteins encoded by other gene family members. It plays an important role in zinc and copper homeostasis, and is induced under hypoxic conditions. The encoded protein is a growth inhibitory factor, and reduced levels of the protein are observed in the brains of individuals with some metal-linked neurodegenerative disorders such as Alzheimer's disease. [provided by RefSeq, Sep 2017].
Disorder MIM:	139255

mRNA and Protein(s)

mRNA	Protein	Name
NM_005954.3	NP_005945.1	metallothionein-3
NM_005954.4	NP_005945.1	metallothionein-3
NM_005954.2	NP_005945.1	metallothionein-3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MT3	XP_001139417.1
Homo sapiens (human)	MT3	NP_005945.1

Gene Ontology
Bibliography

Related articles in PubMed

Mammalian Metallothionein-3: New Functional and Structural Insights.
Va??k M, Meloni G
International journal of molecular sciences18(6)(2017 May)

The unique C- and N-terminal sequences of Metallothionein isoform 3 mediate growth inhibition and Vectorial active transport in MCF-7 cells.
Voels B, Wang L, Sens DA, Garrett SH, Zhang K, Somji S
BMC cancer17(1)369(2017 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The expression of MT-3 mRNA in breast cancer cell lines was significantly lower than in the normal human breast epithelial cell line. The results suggest that MT-3 may play a role in the malignant transformation of breast epithelial cells and in tumor progression.
Title: Expression of metallothionein?3 in ductal breast cancer.

The present study was undertaken to explore further the interrelationship between p53 and metallothioneins.
Title: Basal and copper-induced expression of metallothionein isoform 1,2 and 3 genes in epithelial cancer cells: The role of tumor suppressor p53.

The study implicates the unique C-terminal sequence of MT-3 in the conversion of HK-2 cells to display an enhanced epithelial phenotype.
Title: Cadherin expression, vectorial active transport, and metallothionein isoform 3 mediated EMT/MET responses in cultured primary and immortalized human proximal tubule cells.

MT3 may regulate breast cancer cell invasiveness by modulating the expression of MMP3.
Title: Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.

The epidermis of human skin and resulting malignancies express high level of MT-3.
Title: Metallothionein isoform 3 expression in human skin, related cancers and human skin derived cell cultures.

The following MT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu05258	NM_005954.4 Latest version!	Homo sapiens metallothionein 3 (MT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu05258	NM_005954.3	Homo sapiens metallothionein 3 (MT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu05258	NM_005954.2	Homo sapiens metallothionein 3 (MT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MT3 ( NM_005954.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005945.1 Homo sapiens metallothionein 3 (MT3), mRNA.

Price & Availability↑

CloneID	OHu05258
Clone ID Related Accession (Same CDS sequence)	NM_005954.3 , NM_005954.4 , NM_005954.2
Accession Version	NM_005954.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	metallothionein-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB470290.1, BP210917.1 and CB155600.1. On Sep 5, 2017 this sequence version replaced NM_005954.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP347913.1, BI457719.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCTG AGACCTGCCC CTGCCCTTCT GGTGGCTCCT GCACCTGCGC GGACTCCTGC 
AAGTGCGAGG GATGCAAATG CACCTCCTGC AAGAAGAGCT GCTGCTCCTG CTGCCCTGCG 
GAGTGTGAGA AGTGTGCCAA GGACTGTGTG TGCAAAGGCG GAGAGGCAGC TGAGGCAGAA 
GCAGAGAAGT GCAGCTGCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005945.1
CDS	255..461
Translation	MDPETCPCPSGGSCTCADSCKCEGCKCTSCKKSCCSCCPAECEKCAKDCVCKGGEAAEAEAEKCSCCQ

Target ORF information:

RefSeq Version	NM_005954.3
Organism	Homo sapiens(human)
Definition	Homo sapiens metallothionein 3 (MT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005954.3

ORF Insert Sequence:

ATGGACCCTG AGACCTGCCC CTGCCCTTCT GGTGGCTCCT GCACCTGCGC GGACTCCTGC 
AAGTGCGAGG GATGCAAATG CACCTCCTGC AAGAAGAGCT GCTGCTCCTG CTGCCCTGCG 
GAGTGTGAGA AGTGTGCCAA GGACTGTGTG TGCAAAGGCG GAGAGGCAGC TGAGGCAGAA 
GCAGAGAAGT GCAGCTGCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MT3 ( NM_005954.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005945.1 Homo sapiens metallothionein 3 (MT3), mRNA.

Price & Availability↑

CloneID	OHu05258
Clone ID Related Accession (Same CDS sequence)	NM_005954.3 , NM_005954.4 , NM_005954.2
Accession Version	NM_005954.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-09
Organism	Homo sapiens(human)
Product	metallothionein-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP210917.1 and CB155600.1. On Jun 2, 2019 this sequence version replaced NM_005954.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP211991.1, CK001163.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACCCTG AGACCTGCCC CTGCCCTTCT GGTGGCTCCT GCACCTGCGC GGACTCCTGC 
AAGTGCGAGG GATGCAAATG CACCTCCTGC AAGAAGAGCT GCTGCTCCTG CTGCCCTGCG 
GAGTGTGAGA AGTGTGCCAA GGACTGTGTG TGCAAAGGCG GAGAGGCAGC TGAGGCAGAA 
GCAGAGAAGT GCAGCTGCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005945.1
CDS	64..270
Translation	MDPETCPCPSGGSCTCADSCKCEGCKCTSCKKSCCSCCPAECEKCAKDCVCKGGEAAEAEAEKCSCCQ

Target ORF information:

RefSeq Version	NM_005954.4
Organism	Homo sapiens(human)
Definition	Homo sapiens metallothionein 3 (MT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005954.4

ORF Insert Sequence:

ATGGACCCTG AGACCTGCCC CTGCCCTTCT GGTGGCTCCT GCACCTGCGC GGACTCCTGC 
AAGTGCGAGG GATGCAAATG CACCTCCTGC AAGAAGAGCT GCTGCTCCTG CTGCCCTGCG 
GAGTGTGAGA AGTGTGCCAA GGACTGTGTG TGCAAAGGCG GAGAGGCAGC TGAGGCAGAA 
GCAGAGAAGT GCAGCTGCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MT3 ( NM_005954.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_005945.1 Homo sapiens metallothionein 3 (MT3), mRNA.

Price & Availability↑

CloneID	OHu05258
Clone ID Related Accession (Same CDS sequence)	NM_005954.3 , NM_005954.4 , NM_005954.2
Accession Version	NM_005954.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 207bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-25
Organism	Homo sapiens(Human)
Product	metallothionein-3
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BI820409.1, BI752092.1, BC013081.1 and CB155600.1. On Mar 18, 2004 this sequence version replaced NM_005954.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP347913.1, BI457719.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCCTG AGACCTGCCC CTGCCCTTCT GGTGGCTCCT GCACCTGCGC GGACTCCTGC 
AAGTGCGAGG GATGCAAATG CACCTCCTGC AAGAAGAGCT GCTGCTCCTG CTGCCCTGCG 
GAGTGTGAGA AGTGTGCCAA GGACTGTGTG TGCAAAGGCG GAGAGGCAGC TGAGGCAGAA 
GCAGAGAAGT GCAGCTGCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005945.1
CDS	237..443
Translation	MDPETCPCPSGGSCTCADSCKCEGCKCTSCKKSCCSCCPAECEKCAKDCVCKGGEAAEAEAEKCSCCQ

Target ORF information:

RefSeq Version	NM_005954.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens metallothionein 3 (MT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005954.2

ORF Insert Sequence:

ATGGACCCTG AGACCTGCCC CTGCCCTTCT GGTGGCTCCT GCACCTGCGC GGACTCCTGC 
AAGTGCGAGG GATGCAAATG CACCTCCTGC AAGAAGAGCT GCTGCTCCTG CTGCCCTGCG 
GAGTGTGAGA AGTGTGCCAA GGACTGTGTG TGCAAAGGCG GAGAGGCAGC TGAGGCAGAA 
GCAGAGAAGT GCAGCTGCTG CCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mammalian Metallothionein-3: New Functional and Structural Insights.
International journal of molecular sciences18(6)(2017 May)
Va??k M,Meloni G

The unique C- and N-terminal sequences of Metallothionein isoform 3 mediate growth inhibition and Vectorial active transport in MCF-7 cells.
BMC cancer17(1)369(2017 May)
Voels B,Wang L,Sens DA,Garrett SH,Zhang K,Somji S

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MT3 cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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