ASPA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASPA
Entrez Gene ID	443
Full Name	aspartoacylase
Synonyms	ACY2,ASP
General protein information	Preferred Names aspartoacylase Names aspartoacylase ACY-2 aminoacylase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.2
Summary	This gene encodes an enzyme that catalyzes the conversion of N-acetyl_L-aspartic acid (NAA) to aspartate and acetate. NAA is abundant in the brain where hydrolysis by aspartoacylase is thought to help maintain white matter. This protein is an NAA scavenger in other tissues. Mutations in this gene cause Canavan disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	608034
Disorder Html:	Canavan disease, 271900 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000049.3	NP_000040.1	aspartoacylase
XM_006721527.2	XP_006721590.1	aspartoacylase isoform X1
XM_017024661.1	XP_016880150.1	aspartoacylase isoform X1
NM_001128085.1	NP_001121557.1	aspartoacylase
NM_000049.2	NP_000040.1	aspartoacylase
XM_024450764.1	XP_024306532.1	aspartoacylase isoform X1
NM_000049.4	NP_000040.1	aspartoacylase

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ASPA	XP_001158167.1
	ASPA	XP_001089160.1
Mus musculus (house mouse)	Aspa	NP_075602.2
Rattus norvegicus (Norway rat)	Aspa	NP_077375.1
Danio rerio (zebrafish)	aspa	NP_001103573.1
Homo sapiens (human)	ASPA	NP_001121557.1
Canis lupus familiaris (dog)	ASPA	XP_868535.2
Bos taurus (cattle)	ASPA	NP_001039498.1
Gallus gallus (chicken)	ASPA	XP_415853.3
Xenopus tropicalis (tropical clawed frog)	aspa	NP_001039114.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Aspartoacylase catalytic deficiency as the cause of Canavan disease: a structural perspective.
Wijayasinghe YS, Pavlovsky AG, Viola RE
Biochemistry53(30)4970-8(2014 Aug)

Reexamination of aspartoacylase: is this human enzyme really a glycoprotein?
Wang Q, Viola RE
Archives of biochemistry and biophysics54866-73(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Four ASPA missense mutations associated with Canavan disease are structurally characterized.
Title: Aspartoacylase catalytic deficiency as the cause of Canavan disease: a structural perspective.

Definitive evidence is presented to show that the recombinantly-expressed human aspartoacylase is not a glycoprotein.
Title: Reexamination of aspartoacylase: is this human enzyme really a glycoprotein?

report of 2 Egyptian sibling patients suspected of Canavan disease (CD); study revealed homozygosity for substitution T530C (Ile177Thr) in exon 4 of the ASPA gene in both sibs; substitution T530C (Ile177Thr) results in a novel missense mutation causing a CD phenotype with severe clinical characteristics
Title: New T530C mutation in the aspartoacylase gene caused Canavan disease with no correlation between severity and N-acetylaspartate excretion.

This is the first case report of ASPA mutation studies in Canavan disease from Indian subcontinent.
Title: Molecular characterisation and prenatal diagnosis of Asparto-acylase deficiency (Canavan disease)--report of two novel and two known mutations from the Indian subcontinent.

Human aspartoacylase gene expression was high not only in brain and kidney, but also in lung and liver.
Title: Expression of aspartoacylase (ASPA) and Canavan disease.

The following ASPA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASPA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25884	NM_000049.4 Latest version!	Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu25884	NM_001128085.1 Latest version!	Homo sapiens aspartoacylase (ASPA), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu25884	XM_017024661.1 Latest version!	Homo sapiens aspartoacylase (ASPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu25884	XM_006721527.2 Latest version!	Homo sapiens aspartoacylase (ASPA), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu25884	XM_024450764.1 Latest version!	Homo sapiens aspartoacylase (ASPA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu25884	NM_000049.2	Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu25884	NM_000049.3	Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASPA ( NM_000049.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000040.1 Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25884
Clone ID Related Accession (Same CDS sequence)	NM_000049.3 , XM_006721527.2 , XM_017024661.1 , NM_001128085.1 , NM_000049.2 , XM_024450764.1 , NM_000049.4
Accession Version	NM_000049.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	aspartoacylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025125.15, S67156.1 and BC029128.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000049.2. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.360615.1, SRR1660809.239208.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000040.1
CDS	164..1105
Translation	MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH

Target ORF information:

RefSeq Version	NM_000049.3
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000049.3

ORF Insert Sequence:

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPA ( NM_001128085.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001121557.1 Homo sapiens aspartoacylase (ASPA), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25884
Clone ID Related Accession (Same CDS sequence)	NM_000049.3 , XM_006721527.2 , XM_017024661.1 , NM_001128085.1 , NM_000049.2 , XM_024450764.1 , NM_000049.4
Accession Version	NM_001128085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	aspartoacylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK312901.1 and S67156.1. Transcript Variant: This variant (2) has an alternate 5' UTR and encodes the same protein, as compared to variant 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121557.1
CDS	92..1033
Translation	MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH

Target ORF information:

RefSeq Version	NM_001128085.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartoacylase (ASPA), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128085.1

ORF Insert Sequence:

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPA ( NM_000049.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000040.1 Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25884
Clone ID Related Accession (Same CDS sequence)	NM_000049.3 , XM_006721527.2 , XM_017024661.1 , NM_001128085.1 , NM_000049.2 , XM_024450764.1 , NM_000049.4
Accession Version	NM_000049.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	aspartoacylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from S67156.1 and BC029128.1. On Aug 19, 2005 this sequence version replaced NM_000049.1. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: S67156.1, SRR1803612.138367.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000040.1
CDS	159..1100
Translation	MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH

Target ORF information:

RefSeq Version	NM_000049.2
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000049.2

ORF Insert Sequence:

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPA ( NM_000049.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000040.1 Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25884
Clone ID Related Accession (Same CDS sequence)	NM_000049.3 , XM_006721527.2 , XM_017024661.1 , NM_001128085.1 , NM_000049.2 , XM_024450764.1 , NM_000049.4
Accession Version	NM_000049.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	aspartoacylase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC025125.15. On Aug 31, 2019 this sequence version replaced NM_000049.3. Transcript Variant: This variant (1) and variant 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.142798.1, SRR1803617.130049.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000263080.3/ ENSP00000263080.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000040.1
CDS	164..1105
Translation	MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH

Target ORF information:

RefSeq Version	NM_000049.4
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartoacylase (ASPA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000049.4

ORF Insert Sequence:

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPA ( XM_006721527.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721590.1 Homo sapiens aspartoacylase (ASPA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25884
Clone ID Related Accession (Same CDS sequence)	NM_000049.3 , XM_006721527.2 , XM_017024661.1 , NM_001128085.1 , NM_000049.2 , XM_024450764.1 , NM_000049.4
Accession Version	XM_006721527.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	aspartoacylase isoform X1
Comment

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721590.1
Translation	MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH

Target ORF information:

RefSeq Version	XM_006721527.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens aspartoacylase (ASPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721527.2

ORF Insert Sequence:

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPA ( XM_017024661.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880150.1 Homo sapiens aspartoacylase (ASPA), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu25884
Clone ID Related Accession (Same CDS sequence)	NM_000049.3 , XM_006721527.2 , XM_017024661.1 , NM_001128085.1 , NM_000049.2 , XM_024450764.1 , NM_000049.4
Accession Version	XM_017024661.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aspartoacylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880150.1
CDS	308..1249
Translation	MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH

Target ORF information:

RefSeq Version	XM_017024661.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartoacylase (ASPA), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024661.1

ORF Insert Sequence:

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASPA ( XM_024450764.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306532.1 Homo sapiens aspartoacylase (ASPA), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu25884
Clone ID Related Accession (Same CDS sequence)	NM_000049.3 , XM_006721527.2 , XM_017024661.1 , NM_001128085.1 , NM_000049.2 , XM_024450764.1 , NM_000049.4
Accession Version	XM_024450764.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	aspartoacylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306532.1
CDS	43..984
Translation	MTSCHIAEEHIQKVAIFGGTHGNELTGVFLVKHWLENGAEIQRTGLEVKPFITNPRAVKKCTRYIDCDLNRIFDLENLGKKMSEDLPYEVRRAQEINHLFGPKDSEDSYDIIFDLHNTTSNMGCTLILEDSRNNFLIQMFHYIKTSLAPLPCYVYLIEHPSLKYATTRSIAKYPVGIEVGPQPQGVLRADILDQMRKMIKHALDFIHHFNEGKEFPPCAIEVYKIIEKVDYPRDENGEIAAIIHPNLQDQDWKPLHPGDPMFLTLDGKTIPLGGDCTVYPVFVNEAAYYEKKEAFAKTTKLTLNAKSIRCCLH

Target ORF information:

RefSeq Version	XM_024450764.1
Organism	Homo sapiens(human)
Definition	Homo sapiens aspartoacylase (ASPA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450764.1

ORF Insert Sequence:

ATGACTTCTT GTCACATTGC TGAAGAACAT ATACAAAAGG TTGCTATCTT TGGAGGAACC 
CATGGGAATG AGCTAACCGG AGTATTTCTG GTTAAGCATT GGCTAGAGAA TGGCGCTGAG 
ATTCAGAGAA CAGGGCTGGA GGTAAAACCA TTTATTACTA ACCCCAGAGC AGTGAAGAAG 
TGTACCAGAT ATATTGACTG TGACCTGAAT CGCATTTTTG ACCTTGAAAA TCTTGGCAAA 
AAAATGTCAG AAGATTTGCC ATATGAAGTG AGAAGGGCTC AAGAAATAAA TCATTTATTT 
GGTCCAAAAG ACAGTGAAGA TTCCTATGAC ATTATTTTTG ACCTTCACAA CACCACCTCT 
AACATGGGGT GCACTCTTAT TCTTGAGGAT TCCAGGAATA ACTTTTTAAT TCAGATGTTT 
CATTACATTA AGACTTCTCT GGCTCCACTA CCCTGCTACG TTTATCTGAT TGAGCATCCT 
TCCCTCAAAT ATGCGACCAC TCGTTCCATA GCCAAGTATC CTGTGGGTAT AGAAGTTGGT 
CCTCAGCCTC AAGGGGTTCT GAGAGCTGAT ATCTTGGATC AAATGAGAAA AATGATTAAA 
CATGCTCTTG ATTTTATACA TCATTTCAAT GAAGGAAAAG AATTTCCTCC CTGCGCCATT 
GAGGTCTATA AAATTATAGA GAAAGTTGAT TACCCCCGGG ATGAAAATGG AGAAATTGCT 
GCTATCATCC ATCCTAATCT GCAGGATCAA GACTGGAAAC CACTGCATCC TGGGGATCCC 
ATGTTTTTAA CTCTTGATGG GAAGACGATC CCACTGGGCG GAGACTGTAC CGTGTACCCC 
GTGTTTGTGA ATGAGGCCGC ATATTACGAA AAGAAAGAAG CTTTTGCAAA GACAACTAAA 
CTAACGCTCA ATGCAAAAAG TATTCGCTGC TGTTTACATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Aspartoacylase catalytic deficiency as the cause of Canavan disease: a structural perspective.
Biochemistry53(30)4970-8(2014 Aug)
Wijayasinghe YS,Pavlovsky AG,Viola RE

Reexamination of aspartoacylase: is this human enzyme really a glycoprotein?
Archives of biochemistry and biophysics54866-73(2014 Apr)
Wang Q,Viola RE

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

ASPA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報