EIF2AK4 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011521599.2	XP_011519901.1	eIF-2-alpha kinase GCN2 isoform X2
XM_011521600.3	XP_011519902.1	eIF-2-alpha kinase GCN2 isoform X3
XM_011521600.2	XP_011519902.1	eIF-2-alpha kinase GCN2 isoform X3
NM_001013703.4	NP_001013725.2	eIF-2-alpha kinase GCN2
NM_001013703.3	NP_001013725.2	eIF-2-alpha kinase GCN2
XM_017022219.2	XP_016877708.1	eIF-2-alpha kinase GCN2 isoform X4
XM_005254392.3	XP_005254449.1	eIF-2-alpha kinase GCN2 isoform X1
XM_005254392.2	XP_005254449.1	eIF-2-alpha kinase GCN2 isoform X1
XM_017022219.1	XP_016877708.1	eIF-2-alpha kinase GCN2 isoform X4

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23948	NM_001013703.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu48316	XM_005254392.3 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu80284	XM_011521599.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu80285	XM_011521600.3 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu85425	XM_017022219.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
View Old Accession Versions >>
OHu80285	XM_011521600.2	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu23948	NM_001013703.3	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu48316	XM_005254392.2	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu85425	XM_017022219.1	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
Hide Old Accession Versions >>

EIF2AK4 ( NM_001013703.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013725.2 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.

Price & Availability↑

CloneID	OHu23948
Clone ID Related Accession (Same CDS sequence)	NM_001013703.4 , NM_001013703.3
Accession Version	NM_001013703.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-30
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012377.5, CN286138.1, BC078179.1, AB037759.1, BC014915.1, BC009350.2 and AL157497.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001013703.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology MANE Ensembl match :: ENST00000263791.10/ ENSP00000263791.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TACAAACTCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTCTGGCTGT GGATCTACCC 
AAAGAAACAA TATTACAGTT TTTATCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAAATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTATTTCT GTACAGCTAT 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013725.2
CDS	82..5031
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	NM_001013703.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013703.4

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TACAAACTCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTCTGGCTGT GGATCTACCC 
AAAGAAACAA TATTACAGTT TTTATCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAAATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTATTTCT GTACAGCTAT 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( NM_001013703.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013725.2 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.

Price & Availability↑

CloneID	OHu23948
Clone ID Related Accession (Same CDS sequence)	NM_001013703.4 , NM_001013703.3
Accession Version	NM_001013703.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4950bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN286138.1, AC012377.5, BC078179.1, AB037759.1, BC014915.1, BC009350.2 and AL157497.1. This sequence is a reference standard in the RefSeqGene project. On Mar 6, 2014 this sequence version replaced NM_001013703.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TACAAACTCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTCTGGCTGT GGATCTACCC 
AAAGAAACAA TATTACAGTT TTTATCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAAATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTATTTCT GTACAGCTAT 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013725.2
CDS	73..5022
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	NM_001013703.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013703.3

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TACAAACTCG ACTTCAGACC 
TCCCTTGCCA ACTTACATCA GAAAAGCAGT GAAATTGAAA TTCTGGCTGT GGATCTACCC 
AAAGAAACAA TATTACAGTT TTTATCATTA GAGTGGGATG CTGATGAACA GGCATTTAAC 
ACAACTGTGA AGCAGCTGCT GTCACGCCTG CCAAAGCAAA GATACCTCAA ATTAGTCTGT 
GATGAAATTT ATAACATCAA AGTAGAAAAA AAGGTGTCTG TGCTATTTCT GTACAGCTAT 
AGAGATGACT ACTACAGAAT CTTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_005254392.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254449.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

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CloneID	OHu48316
Clone ID Related Accession (Same CDS sequence)	XM_005254392.3 , XM_005254392.2
Accession Version	XM_005254392.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254392.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TGGATCTACC CAAAGAAACA 
ATATTACAGT TTTTATCATT AGAGTGGGAT GCTGATGAAC AGGCATTTAA CACAACTGTG 
AAGCAGCTGC TGTCACGCCT GCCAAAGCAA AGATACCTCA AATTAGTCTG TGATGAAATT 
TATAACATCA AAGTAGAAAA AAAGGTGTCT GTGCTATTTC TGTACAGCTA TAGAGATGAC 
TACTACAGAA TCTTATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254449.1
CDS	96..4976
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_005254392.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254392.3

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TGGATCTACC CAAAGAAACA 
ATATTACAGT TTTTATCATT AGAGTGGGAT GCTGATGAAC AGGCATTTAA CACAACTGTG 
AAGCAGCTGC TGTCACGCCT GCCAAAGCAA AGATACCTCA AATTAGTCTG TGATGAAATT 
TATAACATCA AAGTAGAAAA AAAGGTGTCT GTGCTATTTC TGTACAGCTA TAGAGATGAC 
TACTACAGAA TCTTATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_005254392.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254449.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

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CloneID	OHu48316
Clone ID Related Accession (Same CDS sequence)	XM_005254392.3 , XM_005254392.2
Accession Version	XM_005254392.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254392.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TGGATCTACC CAAAGAAACA 
ATATTACAGT TTTTATCATT AGAGTGGGAT GCTGATGAAC AGGCATTTAA CACAACTGTG 
AAGCAGCTGC TGTCACGCCT GCCAAAGCAA AGATACCTCA AATTAGTCTG TGATGAAATT 
TATAACATCA AAGTAGAAAA AAAGGTGTCT GTGCTATTTC TGTACAGCTA TAGAGATGAC 
TACTACAGAA TCTTATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254449.1
CDS	96..4976
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_005254392.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254392.2

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGG TGGATCTACC CAAAGAAACA 
ATATTACAGT TTTTATCATT AGAGTGGGAT GCTGATGAAC AGGCATTTAA CACAACTGTG 
AAGCAGCTGC TGTCACGCCT GCCAAAGCAA AGATACCTCA AATTAGTCTG TGATGAAATT 
TATAACATCA AAGTAGAAAA AAAGGTGTCT GTGCTATTTC TGTACAGCTA TAGAGATGAC 
TACTACAGAA TCTTATTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_011521599.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519901.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

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CloneID	OHu80284
Clone ID Related Accession (Same CDS sequence)	XM_011521599.2
Accession Version	XM_011521599.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4836bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521599.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGT GGGATGCTGA TGAACAGGCA 
TTTAACACAA CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAAAGATA CCTCAAATTA 
GTCTGTGATG AAATTTATAA CATCAAAGTA GAAAAAAAGG TGTCTGTGCT ATTTCTGTAC 
AGCTATAGAG ATGACTACTA CAGAATCTTA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519901.1
CDS	96..4931
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_011521599.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521599.2

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTC TGTGTCGACT CTACAAGTTT 
ATTGAACAGA AGGGAGATTT GCAAGATCTT ATGCCAACAA TAAATTCATT AATAAAACAG 
AAAACAGGTA TTGCACAGTT GGTGAAGTAT GGCTTAAAAG ACCTAGAGGA GGTTGTTGGA 
CTGTTGAAGA AACTCGGCAT CAAGTTACAG GTCTTGATCA ATTTGGGCTT GGTTTACAAG 
GTGCAGCAGC ACAATGGAAT CATCTTCCAG TTTGTGGCTT TCATCAAACG AAGGCAAAGG 
GCTGTACCTG AAATCCTCGC AGCTGGAGGC AGATATGACC TGCTGATTCC CCAGTTTAGA 
GGGCCACAAG CTCTGGGGCC AGTTCCCACT GCCATTGGGG TCAGCATAGC TATAGACAAG 
ATATCTGCTG CTGTCCTCAA CATGGAGGAA TCTGTTACAA TAAGCTCTTG TGACCTCCTG 
GTTGTAAGTG TTGGCCAGAT GTCTATGTCC AGGGCCATCA ACCTAACCCA GAAACTCTGG 
ACAGCAGGCA TCACAGCAGA AATCATGTAC GACTGGTCAC AGTCCCAAGA GGAATTACAA 
GAGTACTGCA GACATCATGA AATCACCTAT GTGGCCCTTG TCTCGGATAA AGAAGGAAGC 
CATGTCAAGG TTAAGTCTTT CGAGAAGGAA AGGCAGACAG AGAAGCGTGT GCTGGAGACT 
GAACTTGTGG ACCATGTACT GCAGAAACTG AGGACTAAAG TCACTGATGA AAGGAATGGC 
AGAGAAGCTT CCGATAATCT TGCAGTGCAA AATCTGAAGG GGTCATTTTC TAATGCTTCA 
GGTTTGTTTG AAATCCATGG AGCAACAGTG GTTCCCATTG TGAGTGTGCT AGCCCCGGAG 
AAGCTGTCAG CCAGCACTAG GAGGCGCTAT GAAACTCAGT GGGATGCTGA TGAACAGGCA 
TTTAACACAA CTGTGAAGCA GCTGCTGTCA CGCCTGCCAA AGCAAAGATA CCTCAAATTA 
GTCTGTGATG AAATTTATAA CATCAAAGTA GAAAAAAAGG TGTCTGTGCT ATTTCTGTAC 
AGCTATAGAG ATGACTACTA CAGAATCTTA TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_011521600.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519902.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

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CloneID	OHu80285
Clone ID Related Accession (Same CDS sequence)	XM_011521600.3 , XM_011521600.2
Accession Version	XM_011521600.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011521600.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGGT ACAAACTCGA 
CTTCAGACCT CCCTTGCCAA CTTACATCAG AAAAGCAGTG AAATTGAAAT TCTGGCTGTG 
GATCTACCCA AAGAAACAAT ATTACAGTTT TTATCATTAG AGTGGGATGC TGATGAACAG 
GCATTTAACA CAACTGTGAA GCAGCTGCTG TCACGCCTGC CAAAGCAAAG ATACCTCAAA 
TTAGTCTGTG ATGAAATTTA TAACATCAAA GTAGAAAAAA AGGTGTCTGT GCTATTTCTG 
TACAGCTATA GAGATGACTA CTACAGAATC TTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519902.1
CDS	96..4874
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_011521600.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521600.3

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGGT ACAAACTCGA 
CTTCAGACCT CCCTTGCCAA CTTACATCAG AAAAGCAGTG AAATTGAAAT TCTGGCTGTG 
GATCTACCCA AAGAAACAAT ATTACAGTTT TTATCATTAG AGTGGGATGC TGATGAACAG 
GCATTTAACA CAACTGTGAA GCAGCTGCTG TCACGCCTGC CAAAGCAAAG ATACCTCAAA 
TTAGTCTGTG ATGAAATTTA TAACATCAAA GTAGAAAAAA AGGTGTCTGT GCTATTTCTG 
TACAGCTATA GAGATGACTA CTACAGAATC TTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_011521600.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011519902.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu80285
Clone ID Related Accession (Same CDS sequence)	XM_011521600.3 , XM_011521600.2
Accession Version	XM_011521600.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4779bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521600.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGGT ACAAACTCGA 
CTTCAGACCT CCCTTGCCAA CTTACATCAG AAAAGCAGTG AAATTGAAAT TCTGGCTGTG 
GATCTACCCA AAGAAACAAT ATTACAGTTT TTATCATTAG AGTGGGATGC TGATGAACAG 
GCATTTAACA CAACTGTGAA GCAGCTGCTG TCACGCCTGC CAAAGCAAAG ATACCTCAAA 
TTAGTCTGTG ATGAAATTTA TAACATCAAA GTAGAAAAAA AGGTGTCTGT GCTATTTCTG 
TACAGCTATA GAGATGACTA CTACAGAATC TTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011519902.1
CDS	96..4874
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_011521600.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521600.2

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGGT ACAAACTCGA 
CTTCAGACCT CCCTTGCCAA CTTACATCAG AAAAGCAGTG AAATTGAAAT TCTGGCTGTG 
GATCTACCCA AAGAAACAAT ATTACAGTTT TTATCATTAG AGTGGGATGC TGATGAACAG 
GCATTTAACA CAACTGTGAA GCAGCTGCTG TCACGCCTGC CAAAGCAAAG ATACCTCAAA 
TTAGTCTGTG ATGAAATTTA TAACATCAAA GTAGAAAAAA AGGTGTCTGT GCTATTTCTG 
TACAGCTATA GAGATGACTA CTACAGAATC TTATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_017022219.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877708.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu85425
Clone ID Related Accession (Same CDS sequence)	XM_017022219.2 , XM_017022219.1
Accession Version	XM_017022219.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017022219.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGTG GGATGCTGAT 
GAACAGGCAT TTAACACAAC TGTGAAGCAG CTGCTGTCAC GCCTGCCAAA GCAAAGATAC 
CTCAAATTAG TCTGTGATGA AATTTATAAC ATCAAAGTAG AAAAAAAGGT GTCTGTGCTA 
TTTCTGTACA GCTATAGAGA TGACTACTAC AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877708.1
CDS	96..4760
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_017022219.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022219.2

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGTG GGATGCTGAT 
GAACAGGCAT TTAACACAAC TGTGAAGCAG CTGCTGTCAC GCCTGCCAAA GCAAAGATAC 
CTCAAATTAG TCTGTGATGA AATTTATAAC ATCAAAGTAG AAAAAAAGGT GTCTGTGCTA 
TTTCTGTACA GCTATAGAGA TGACTACTAC AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF2AK4 ( XM_017022219.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877708.1 Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

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CloneID	OHu85425
Clone ID Related Accession (Same CDS sequence)	XM_017022219.2 , XM_017022219.1
Accession Version	XM_017022219.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	eIF-2-alpha kinase GCN2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGTG GGATGCTGAT 
GAACAGGCAT TTAACACAAC TGTGAAGCAG CTGCTGTCAC GCCTGCCAAA GCAAAGATAC 
CTCAAATTAG TCTGTGATGA AATTTATAAC ATCAAAGTAG AAAAAAAGGT GTCTGTGCTA 
TTTCTGTACA GCTATAGAGA TGACTACTAC AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877708.1
CDS	96..4760
Translation	MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF

Target ORF information:

RefSeq Version	XM_017022219.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 4 (EIF2AK4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022219.1

ORF Insert Sequence:

ATGGCTGGGG GCCGTGGGGC CCCCGGGCGC GGCCGGGACG AGCCTCCGGA GAGCTACCCG 
CAACGACAGG ACCACGAGCT ACAGGCCCTG GAGGCCATTT ACGGCGCGGA CTTCCAAGAC 
CTGCGGCCGG ACGCTTGCGG ACCGGTCAAA GAGCCCCCTG AAATCAATTT AGTTTTGTAC 
CCTCAAGGCC TAACTGGTGA AGAAGTATAT GTAAAAGTGG ATTTGAGGGT TAAATGCCCA 
CCTACCTATC CAGATGTAGT TCCTGAAATA GAGTTAAAAA ATGCCAAAGG TCTATCAAAT 
GAAAGTGTCA ATTTGTTAAA ATCTCGCCTA GAAGAACTGG CCAAGAAACA CTGTGGGGAG 
GTGATGATCT TTGAACTGGC TTACCACGTG CAGTCATTTC TCAGCGAGCA TAACAAGCCC 
CCTCCCAAGT CTTTTCATGA AGAAATGCTG GAAAGGCGGG CTCAGGAGGA GCAGCAGAGG 
CTGTTGGAGG CCAAGCGGAA AGAAGAGCAG GAGCAACGTG AAATCCTGCA TGAGATTCAG 
AGAAGGAAAG AAGAGATAAA AGAAGAGAAA AAAAGGAAAG AAATGGCTAA GCAGGAACGT 
TTGGAAATTG CTAGTTTGTC AAACCAAGAT CATACCTCTA AGAAGGACCC AGGAGGACAC 
AGAACGGCTG CCATTCTACA TGGAGGCTCT CCTGACTTTG TAGGAAATGG TAAACATCGG 
GCAAACTCCT CAGGAAGGTC TAGGCGAGAA CGTCAGTATT CTGTATGTAA TAGTGAAGAT 
TCTCCTGGCT CTTGTGAAAT TCTGTATTTC AATATGGGGA GTCCTGATCA GCTCATGGTG 
CACAAAGGGA AATGTATTGG CAGTGATGAA CAACTTGGAA AATTAGTCTA CAATGCTTTG 
GAAACAGCCA CTGGTGGCTT TGTCTTGTTG TATGAGTGGG TCCTTCAGTG GCAGAAAAAA 
ATGGGTCCAT TCCTTACCAG TCAAGAAAAA GAGAAGATTG ATAAGTGCAA AAAGCAGATT 
CAAGGAACAG AAACAGAATT CAACTCACTG GTAAAATTGA GCCATCCAAA TGTAGTACGC 
TACCTTGCAA TGAATCTCAA AGAGCAAGAC GACTCCATCG TGGTGGACAT TTTAGTGGAG 
CACATTAGTG GGGTCTCTCT TGCTGCACAC CTGAGCCACT CAGGCCCCAT CCCTGTGCAT 
CAGCTTCGCA GGTACACAGC TCAGCTCCTG TCAGGCCTTG ATTATCTGCA CAGCAATTCT 
GTGGTGCATA AGGTCCTGAG TGCATCTAAT GTCTTGGTGG ATGCAGAAGG CACCGTCAAG 
ATTACGGACT ATAGCATTTC TAAGCGCCTC GCAGACATTT GCAAGGAGGA TGTGTTTGAG 
CAAACCCGAG TTCGTTTTAG TGACAATGCT CTGCCTTATA AAACGGGGAA GAAAGGAGAT 
GTTTGGCGTC TTGGCCTTCT GCTGCTGTCC CTCAGCCAAG GACAGGAATG TGGAGAGTAC 
CCTGTGACCA TCCCTAGTGA CTTACCAGCT GACTTTCAAG ATTTTCTAAA GAAATGTGTG 
TGCTTGGATG ACAAGGAAAG ATGGAGTCCC CAGCAGTTGT TGAAACACAG CTTTATAAAT 
CCCCAGCCAA AAATGCCTCT AGTGGAACAA AGTCCTGAAG ATTCTGAAGG ACAAGATTAT 
GTTGAGACTG TTATTCCTAG CAACCGGCTA CCCAGTGCTG CCTTCTTTAG TGAGACACAG 
AGACAGTTTT CCCGATACTT CATTGAGTTT GAAGAATTAC AACTTCTTGG TAAAGGAGCT 
TTTGGAGCTG TCATCAAGGT GCAGAACAAG TTGGACGGCT GCTGCTACGC AGTGAAGCGC 
ATCCCCATCA ACCCGGCCAG CCGGCAGTTC CGCAGGATCA AGGGCGAAGT GACACTGCTG 
TCACGGCTGC ACCATGAGAA CATTGTGCGC TACTACAACG CCTGGATCGA GCGGCACGAG 
CGGCCGGCGG GACCGGGGAC GCCGCCCCCG GACTCCGGGC CCCTGGCCAA GGATGACCGA 
GCTGCACGCG GGCAGCCGGC GAGCGACACA GACGGCCTGG ACAGCGTAGA GGCCGCCGCG 
CCGCCACCCA TCCTCAGCAG CTCGGTGGAG TGGAGCACTT CGGGCGAGCG CTCGGCCAGT 
GCCCGTTTCC CCGCCACCGG CCCGGGCTCC AGCGATGACG AGGACGACGA CGAGGACGAG 
CACGGTGGCG TCTTCTCCCA GTCCTTCCTG CCTGCTTCAG ATTCTGAAAG TGATATTATC 
TTTGACAATG AAGATGAGAA CAGTAAAAGT CAGAATCAGG ATGAAGATTG CAATGAAAAG 
AATGGCTGCC ATGAAAGTGA GCCATCAGTG ACGACTGAGG CTGTGCACTA CCTATACATC 
CAGATGGAGT ACTGTGAGAA GAGCACTTTA CGAGACACCA TTGACCAGGG ACTGTATCGA 
GACACCGTCA GACTCTGGAG GCTTTTTCGA GAGATTCTGG ATGGATTAGC TTATATCCAT 
GAGAAAGGAA TGATTCACCG GGATTTGAAG CCTGTCAACA TTTTTTTGGA TTCTGATGAC 
CATGTGAAAA TAGGTGATTT TGGTTTGGCG ACAGACCATC TAGCCTTTTC TGCTGACAGC 
AAACAAGACG ATCAGACAGG AGACTTGATT AAGTCAGACC CTTCAGGTCA CTTAACTGGG 
ATGGTTGGCA CTGCTCTCTA TGTAAGCCCA GAGGTCCAAG GAAGCACCAA ATCTGCATAC 
AACCAGAAAG TGGATCTCTT CAGCCTGGGA ATTATCTTCT TTGAGATGTC CTATCACCCC 
ATGGTCACGG CTTCAGAAAG GATCTTTGTT CTCAACCAAC TCAGAGATCC CACTTCGCCT 
AAGTTTCCAG AAGACTTTGA CGATGGAGAG CATGCAAAGC AGAAATCAGT CATCTCCTGG 
CTGTTGAACC ACGATCCAGC AAAACGGCCC ACAGCCACAG AACTGCTCAA GAGTGAGCTG 
CTGCCCCCAC CCCAGATGGA GGAGTCAGAG CTGCATGAAG TGCTGCACCA CACGCTGACC 
AACGTGGATG GGAAGGCCTA CCGCACCATG ATGGCCCAGA TCTTCTCGCA GCGCATCTCC 
CCTGCCATCG ATTACACCTA TGACAGCGAC ATACTGAAGG GCAACTTCTC AATCCGTACA 
GCCAAGATGC AGCAGCATGT GTGTGAAACC ATCATCCGCA TCTTTAAAAG ACATGGAGCT 
GTTCAGTTGT GTACTCCACT ACTGCTTCCC CGAAACAGAC AAATATATGA GCACAACGAA 
GCTGCCCTAT TCATGGACCA CAGCGGGATG CTGGTGATGC TTCCTTTTGA CCTGCGGATC 
CCTTTTGCAA GATATGTGGC AAGAAATAAT ATATTGAATT TAAAACGATA CTGCATAGAA 
CGTGTGTTCA GGCCGCGCAA GTTAGATCGA TTTCATCCCA AAGAACTTCT GGAGTGTGCA 
TTTGATATTG TCACTTCTAC CACCAACAGC TTTCTGCCCA CTGCTGAAAT TATCTACACT 
ATCTATGAAA TCATCCAAGA GTTTCCAGCA CTTCAGGAAA GAAATTACAG TATTTATTTG 
AACCATACCA TGTTATTGAA AGCAATACTC TTACACTGTG GGATCCCAGA AGATAAACTC 
AGTCAAGTCT ACATTATTCT GTATGATGCT GTGACAGAGA AGCTGACGAG GAGAGAAGTG 
GAAGCTAAAT TTTGTAATCT GTCTTTGTCT TCTAATAGTG TCTTGATCAA TTTGGGCTTG 
GTTTACAAGG TGCAGCAGCA CAATGGAATC ATCTTCCAGT TTGTGGCTTT CATCAAACGA 
AGGCAAAGGG CTGTACCTGA AATCCTCGCA GCTGGAGGCA GATATGACCT GCTGATTCCC 
CAGTTTAGAG GGCCACAAGC TCTGGGGCCA GTTCCCACTG CCATTGGGGT CAGCATAGCT 
ATAGACAAGA TATCTGCTGC TGTCCTCAAC ATGGAGGAAT CTGTTACAAT AAGCTCTTGT 
GACCTCCTGG TTGTAAGTGT TGGCCAGATG TCTATGTCCA GGGCCATCAA CCTAACCCAG 
AAACTCTGGA CAGCAGGCAT CACAGCAGAA ATCATGTACG ACTGGTCACA GTCCCAAGAG 
GAATTACAAG AGTACTGCAG ACATCATGAA ATCACCTATG TGGCCCTTGT CTCGGATAAA 
GAAGGAAGCC ATGTCAAGGT TAAGTCTTTC GAGAAGGAAA GGCAGACAGA GAAGCGTGTG 
CTGGAGACTG AACTTGTGGA CCATGTACTG CAGAAACTGA GGACTAAAGT CACTGATGAA 
AGGAATGGCA GAGAAGCTTC CGATAATCTT GCAGTGCAAA ATCTGAAGGG GTCATTTTCT 
AATGCTTCAG GTTTGTTTGA AATCCATGGA GCAACAGTGG TTCCCATTGT GAGTGTGCTA 
GCCCCGGAGA AGCTGTCAGC CAGCACTAGG AGGCGCTATG AAACTCAGTG GGATGCTGAT 
GAACAGGCAT TTAACACAAC TGTGAAGCAG CTGCTGTCAC GCCTGCCAAA GCAAAGATAC 
CTCAAATTAG TCTGTGATGA AATTTATAAC ATCAAAGTAG AAAAAAAGGT GTCTGTGCTA 
TTTCTGTACA GCTATAGAGA TGACTACTAC AGAATCTTAT TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	EIF2AK4
Entrez Gene ID	440275
Full Name	eukaryotic translation initiation factor 2 alpha kinase 4
Synonyms	GCN2,PVOD2
General protein information	Preferred Names eukaryotic translation initiation factor 2 alpha kinase 4 Names eIF-2-alpha kinase GCN2 GCN2 eIF2alpha kinase GCN2-like protein general control nonderepressible 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	This gene encodes a member of a family of kinases that phosphorylate the alpha subunit of eukaryotic translation initiation factor-2 (EIF2), resulting in the downregulaton of protein synthesis. The encoded protein responds to amino acid deprivation by binding uncharged transfer RNAs. It may also be activated by glucose deprivation and viral infection. Mutations in this gene have been found in individuals suffering from autosomal recessive pulmonary venoocclusive-disease-2. [provided by RefSeq, Mar 2014].
Disorder MIM:	609280
Disorder Html:	Pulmonary venoocclusive disease 2, 234810 (3)

Pan troglodytes (chimpanzee)	EIF2AK4	XP_001140245.1
	EIF2AK4	XP_002804763.1
Gallus gallus (chicken)	EIF2AK4	XP_004941777.1
Eremothecium gossypii	AGOS_AAL083W	NP_982459.1
Rattus norvegicus (Norway rat)	Eif2ak4	NP_001099214.2
Danio rerio (zebrafish)	eif2ak4	XP_002667149.3
Drosophila melanogaster (fruit fly)	Gcn2	NP_001263141.1
Saccharomyces cerevisiae (baker's yeast)	GCN2	NP_010569.3
Kluyveromyces lactis	KLLA0C10802g	XP_452681.1
Neurospora crassa	NCU01187	XP_961553.1
Xenopus tropicalis (tropical clawed frog)	eif2ak4	NP_001243855.1
Homo sapiens (human)	EIF2AK4	NP_001013725.2
Canis lupus familiaris (dog)	EIF2AK4	XP_535431.3
Bos taurus (cattle)	EIF2AK4	XP_002690831.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012368	XP_320188.4
Caenorhabditis elegans (roundworm)	gcn-2	NP_001254370.1
Schizosaccharomyces pombe (fission yeast)	gcn2	NP_595991.2
Mus musculus (house mouse)	Eif2ak4	NP_038747.2
Magnaporthe oryzae (rice blast fungus)	MGG_08643	XP_003711074.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

EIF2AK4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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