ASL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASL
Entrez Gene ID	435
Full Name	argininosuccinate lyase
Synonyms	ASAL
General protein information	Preferred Names argininosuccinate lyase Names argininosuccinate lyase argininosuccinase arginosuccinase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q11.21
Summary	This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008].
Disorder MIM:	608310
Disorder Html:	Argininosuccinic aciduria, 207900 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024944.1	NP_001020115.1	argininosuccinate lyase isoform 2
NM_001024946.2	NP_001020117.1	argininosuccinate lyase isoform 3
NM_001024946.1	NP_001020117.1	argininosuccinate lyase isoform 3
NM_000048.3	NP_000039.2	argininosuccinate lyase isoform 1
NM_000048.4	NP_000039.2	argininosuccinate lyase isoform 1
NM_001024943.1	NP_001020114.1	argininosuccinate lyase isoform 1
NM_001024944.2	NP_001020115.1	argininosuccinate lyase isoform 2
NM_001024943.2	NP_001020114.1	argininosuccinate lyase isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ASL	NP_001029600.1
Gallus gallus (chicken)	ASL2	NP_001025885.1
Saccharomyces cerevisiae (baker's yeast)	ARG4	NP_011882.1
Rattus norvegicus (Norway rat)	Asl	NP_067588.2
Drosophila melanogaster (fruit fly)	CG9510	NP_001027234.1
Eremothecium gossypii	AGOS_AEL019W	NP_984842.1
Magnaporthe oryzae (rice blast fungus)	MGG_17278	XP_003717639.1
Homo sapiens (human)	ASL	NP_000039.2
Pan troglodytes (chimpanzee)	ASL	XP_519125.3
Mus musculus (house mouse)	Asl	NP_598529.1
Oryza sativa (rice)	Os03g0305500	NP_001049883.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008141	XP_317320.4
Canis lupus familiaris (dog)	LOC479704	XP_536832.2
Gallus gallus (chicken)	ASL1	NP_990832.1
Danio rerio (zebrafish)	asl	NP_956745.1
Kluyveromyces lactis	KLLA0A08404g	XP_451371.1
Schizosaccharomyces pombe (fission yeast)	SPBC1539.03c	NP_596817.1
Schizosaccharomyces pombe (fission yeast)	arg7	NP_595129.1
Neurospora crassa	NCU08162	XP_959654.1
Arabidopsis thaliana (thale cress)	AT5G10920	NP_196653.1
Xenopus tropicalis (tropical clawed frog)	asl	NP_001106586.1

Gene Ontology
Bibliography

Related articles in PubMed

[Mutational analysis of ASS1, ASL and SLC25A13 genes in six Chinese patients with citrullinemia].
Lin Y, Yu K, Li L, Zheng Z, Lin W, Fu Q
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(5)676-679(2017 Oct)

Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells.
Hung YH, Huang HL, Chen WC, Yen MC, Cho CY, Weng TY, Wang CY, Chen YL, Chen LT, Lai MD
Oncology reports37(2)969-978(2017 Feb)

Silencing of argininosuccinate lyase inhibits colorectal cancer formation.
Huang HL, Chen WC, Hsu HP, Cho CY, Hung YH, Wang CY, Lai MD
Oncology reports37(1)163-170(2017 Jan)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Overexpression of ASL may be a contributing factor in drug resistance for arginine deprivation therapy.
Title: Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells.

ASL-targeting shRNA-induced growth inhibition is associated with decreased cyclin A2 expression and Nitric oxide content in colon cancer.
Title: Silencing of argininosuccinate lyase inhibits colorectal cancer formation.

the mechanism induced by ASL shRNA which occurred in human breast cancer may be attributed to a decrease in cyclin A2 and NO.
Title: Argininosuccinate lyase is a potential therapeutic target in breast cancer.

The clinical and biochemical course in variant forms of ASL deficiency is associated with relevant residual levels of ASL activity as well as instability of mutant ASL proteins.
Title: Unstable argininosuccinate lyase in variant forms of the urea cycle disorder argininosuccinic aciduria.

Point mutation of ASS1, ASL and SLC25A13 is associated with citrullinemia.
Title: [Genetic analysis of ASS1, ASL and SLC25A13 in citrullinemia patients].

The following ASL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22033	NM_001024943.2 Latest version!	Homo sapiens argininosuccinate lyase (ASL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22033	NM_000048.4 Latest version!	Homo sapiens argininosuccinate lyase (ASL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22282	NM_001024946.2 Latest version!	Homo sapiens argininosuccinate lyase (ASL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OHu23053	NM_001024944.2 Latest version!	Homo sapiens argininosuccinate lyase (ASL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu22282	NM_001024946.1	Homo sapiens argininosuccinate lyase (ASL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
OHu22033	NM_000048.3	Homo sapiens argininosuccinate lyase (ASL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu23053	NM_001024944.1	Homo sapiens argininosuccinate lyase (ASL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu22033	NM_001024943.1	Homo sapiens argininosuccinate lyase (ASL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASL ( NM_000048.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000039.2 Homo sapiens argininosuccinate lyase (ASL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22033
Clone ID Related Accession (Same CDS sequence)	NM_000048.3 , NM_000048.4 , NM_001024943.1 , NM_001024943.2
Accession Version	NM_000048.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX328836.2, BC008195.1, AC068533.7 and AI469765.1. This sequence is a reference standard in the RefSeqGene project. On Jun 29, 2005 this sequence version replaced NM_000048.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.68956.1, SRR1803612.193225.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000039.2
CDS	112..1506
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_000048.3
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000048.3

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASL ( NM_000048.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000039.2 Homo sapiens argininosuccinate lyase (ASL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22033
Clone ID Related Accession (Same CDS sequence)	NM_000048.3 , NM_000048.4 , NM_001024943.1 , NM_001024943.2
Accession Version	NM_000048.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX328836.2, BC008195.1, AC068533.7 and AI469765.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_000048.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.193225.1, SRR7410570.272416.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000304874.14/ ENSP00000307188.9 RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000039.2
CDS	82..1476
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_000048.4
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000048.4

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASL ( NM_001024943.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020114.1 Homo sapiens argininosuccinate lyase (ASL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22033
Clone ID Related Accession (Same CDS sequence)	NM_000048.3 , NM_000048.4 , NM_001024943.1 , NM_001024943.2
Accession Version	NM_001024943.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ949510.1, AC068533.7 and AI469765.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.32182.1, SRR1803615.48051.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020114.1
CDS	236..1630
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_001024943.1
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024943.1

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASL ( NM_001024943.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020114.1 Homo sapiens argininosuccinate lyase (ASL), transcript variant 1, mRNA.

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CloneID	OHu22033
Clone ID Related Accession (Same CDS sequence)	NM_000048.3 , NM_000048.4 , NM_001024943.1 , NM_001024943.2
Accession Version	NM_001024943.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1395bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068533.7, BQ949510.1 and AI469765.1. On May 31, 2019 this sequence version replaced NM_001024943.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.32182.1, SRR1803615.48051.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020114.1
CDS	264..1658
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_001024943.2
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024943.2

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGTG TGCCGGGCTC CTGATGACCC TCAAGGGACT TCCCAGCACC 
TACAACAAAG ACTTACAGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASL ( NM_001024946.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020117.1 Homo sapiens argininosuccinate lyase (ASL), transcript variant 4, mRNA.

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CloneID	OHu22282
Clone ID Related Accession (Same CDS sequence)	NM_001024946.2 , NM_001024946.1
Accession Version	NM_001024946.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068533.7, BQ949510.1 and AI469765.1. On Jun 1, 2019 this sequence version replaced NM_001024946.1. Transcript Variant: This variant (4) lacks an alternate, in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.56161.1, KU177921.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGTGGGGC CATTGCAGGC 
AATCCCCTGG GTGTGGACCG AGAGCTGCTC CGAGCAGAAC TCAACTTTGG GGCCATCACT 
CTCAACAGCA TGGATGCCAC TAGTGAGCGG GACTTTGTGG CCGAGTTCCT GTTCTGGGCT 
TCGCTGTGCA TGACCCATCT CAGCAGGATG GCCGAGGACC TCATCCTCTA CTGCACCAAG 
GAATTCAGCT TCGTGCAGCT CTCAGATGCC TACAGCACGG GAAGCAGCCT GATGCCCCAG 
AAGAAAAACC CCGACAGTTT GGAGCTGATC CGGAGCAAGG CTGGGCGTGT GTTTGGGCGG 
TGTGCCGGGC TCCTGATGAC CCTCAAGGGA CTTCCCAGCA CCTACAACAA AGACTTACAG 
GAGGACAAGG AAGCTGTGTT TGAAGTGTCA GACACTATGA GTGCCGTGCT CCAGGTGGCC 
ACTGGCGTCA TCTCTACGCT GCAGATTCAC CAAGAGAACA TGGGACAGGC TCTCAGCCCC 
GACATGCTGG CCACTGACCT TGCCTATTAC CTGGTCCGCA AAGGGATGCC ATTCCGCCAG 
GCCCACGAGG CCTCCGGGAA AGCTGTGTTC ATGGCCGAGA CCAAGGGGGT CGCCCTCAAC 
CAGCTGTCAC TGCAGGAGCT GCAGACCATC AGCCCCCTGT TCTCGGGCGA CGTGATCTGC 
GTGTGGGACT ACGGGCACAG TGTGGAGCAG TATGGTGCCC TGGGCGGCAC TGCGCGCTCC 
AGCGTCGACT GGCAGATCCG CCAGGTGCGG GCGCTACTGC AGGCACAGCA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020117.1
CDS	264..1580
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_001024946.2
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024946.2

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGTGGGGC CATTGCAGGC 
AATCCCCTGG GTGTGGACCG AGAGCTGCTC CGAGCAGAAC TCAACTTTGG GGCCATCACT 
CTCAACAGCA TGGATGCCAC TAGTGAGCGG GACTTTGTGG CCGAGTTCCT GTTCTGGGCT 
TCGCTGTGCA TGACCCATCT CAGCAGGATG GCCGAGGACC TCATCCTCTA CTGCACCAAG 
GAATTCAGCT TCGTGCAGCT CTCAGATGCC TACAGCACGG GAAGCAGCCT GATGCCCCAG 
AAGAAAAACC CCGACAGTTT GGAGCTGATC CGGAGCAAGG CTGGGCGTGT GTTTGGGCGG 
TGTGCCGGGC TCCTGATGAC CCTCAAGGGA CTTCCCAGCA CCTACAACAA AGACTTACAG 
GAGGACAAGG AAGCTGTGTT TGAAGTGTCA GACACTATGA GTGCCGTGCT CCAGGTGGCC 
ACTGGCGTCA TCTCTACGCT GCAGATTCAC CAAGAGAACA TGGGACAGGC TCTCAGCCCC 
GACATGCTGG CCACTGACCT TGCCTATTAC CTGGTCCGCA AAGGGATGCC ATTCCGCCAG 
GCCCACGAGG CCTCCGGGAA AGCTGTGTTC ATGGCCGAGA CCAAGGGGGT CGCCCTCAAC 
CAGCTGTCAC TGCAGGAGCT GCAGACCATC AGCCCCCTGT TCTCGGGCGA CGTGATCTGC 
GTGTGGGACT ACGGGCACAG TGTGGAGCAG TATGGTGCCC TGGGCGGCAC TGCGCGCTCC 
AGCGTCGACT GGCAGATCCG CCAGGTGCGG GCGCTACTGC AGGCACAGCA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASL ( NM_001024946.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020117.1 Homo sapiens argininosuccinate lyase (ASL), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22282
Clone ID Related Accession (Same CDS sequence)	NM_001024946.2 , NM_001024946.1
Accession Version	NM_001024946.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ949510.1, AC068533.7 and AI469765.1. Transcript Variant: This variant (4) lacks an alternate, in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.56161.1, ERR279838.7571.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGTGGGGC CATTGCAGGC 
AATCCCCTGG GTGTGGACCG AGAGCTGCTC CGAGCAGAAC TCAACTTTGG GGCCATCACT 
CTCAACAGCA TGGATGCCAC TAGTGAGCGG GACTTTGTGG CCGAGTTCCT GTTCTGGGCT 
TCGCTGTGCA TGACCCATCT CAGCAGGATG GCCGAGGACC TCATCCTCTA CTGCACCAAG 
GAATTCAGCT TCGTGCAGCT CTCAGATGCC TACAGCACGG GAAGCAGCCT GATGCCCCAG 
AAGAAAAACC CCGACAGTTT GGAGCTGATC CGGAGCAAGG CTGGGCGTGT GTTTGGGCGG 
TGTGCCGGGC TCCTGATGAC CCTCAAGGGA CTTCCCAGCA CCTACAACAA AGACTTACAG 
GAGGACAAGG AAGCTGTGTT TGAAGTGTCA GACACTATGA GTGCCGTGCT CCAGGTGGCC 
ACTGGCGTCA TCTCTACGCT GCAGATTCAC CAAGAGAACA TGGGACAGGC TCTCAGCCCC 
GACATGCTGG CCACTGACCT TGCCTATTAC CTGGTCCGCA AAGGGATGCC ATTCCGCCAG 
GCCCACGAGG CCTCCGGGAA AGCTGTGTTC ATGGCCGAGA CCAAGGGGGT CGCCCTCAAC 
CAGCTGTCAC TGCAGGAGCT GCAGACCATC AGCCCCCTGT TCTCGGGCGA CGTGATCTGC 
GTGTGGGACT ACGGGCACAG TGTGGAGCAG TATGGTGCCC TGGGCGGCAC TGCGCGCTCC 
AGCGTCGACT GGCAGATCCG CCAGGTGCGG GCGCTACTGC AGGCACAGCA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020117.1
CDS	236..1552
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGRCAGLLMTLKGLPSTYNKDLQEDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_001024946.1
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024946.1

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGTGGGGC CATTGCAGGC 
AATCCCCTGG GTGTGGACCG AGAGCTGCTC CGAGCAGAAC TCAACTTTGG GGCCATCACT 
CTCAACAGCA TGGATGCCAC TAGTGAGCGG GACTTTGTGG CCGAGTTCCT GTTCTGGGCT 
TCGCTGTGCA TGACCCATCT CAGCAGGATG GCCGAGGACC TCATCCTCTA CTGCACCAAG 
GAATTCAGCT TCGTGCAGCT CTCAGATGCC TACAGCACGG GAAGCAGCCT GATGCCCCAG 
AAGAAAAACC CCGACAGTTT GGAGCTGATC CGGAGCAAGG CTGGGCGTGT GTTTGGGCGG 
TGTGCCGGGC TCCTGATGAC CCTCAAGGGA CTTCCCAGCA CCTACAACAA AGACTTACAG 
GAGGACAAGG AAGCTGTGTT TGAAGTGTCA GACACTATGA GTGCCGTGCT CCAGGTGGCC 
ACTGGCGTCA TCTCTACGCT GCAGATTCAC CAAGAGAACA TGGGACAGGC TCTCAGCCCC 
GACATGCTGG CCACTGACCT TGCCTATTAC CTGGTCCGCA AAGGGATGCC ATTCCGCCAG 
GCCCACGAGG CCTCCGGGAA AGCTGTGTTC ATGGCCGAGA CCAAGGGGGT CGCCCTCAAC 
CAGCTGTCAC TGCAGGAGCT GCAGACCATC AGCCCCCTGT TCTCGGGCGA CGTGATCTGC 
GTGTGGGACT ACGGGCACAG TGTGGAGCAG TATGGTGCCC TGGGCGGCAC TGCGCGCTCC 
AGCGTCGACT GGCAGATCCG CCAGGTGCGG GCGCTACTGC AGGCACAGCA GGCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASL ( NM_001024944.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020115.1 Homo sapiens argininosuccinate lyase (ASL), transcript variant 3, mRNA.

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CloneID	OHu23053
Clone ID Related Accession (Same CDS sequence)	NM_001024944.1 , NM_001024944.2
Accession Version	NM_001024944.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ949510.1, AC068533.7, BU634268.1 and AI469765.1. Transcript Variant: This variant (3) lacks an alternate, in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020115.1
CDS	236..1570
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGREDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_001024944.1
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024944.1

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASL ( NM_001024944.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001020115.1 Homo sapiens argininosuccinate lyase (ASL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu23053
Clone ID Related Accession (Same CDS sequence)	NM_001024944.1 , NM_001024944.2
Accession Version	NM_001024944.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1335bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	argininosuccinate lyase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068533.7, BQ949510.1, BU634268.1 and AI469765.1. On May 31, 2019 this sequence version replaced NM_001024944.1. Transcript Variant: This variant (3) lacks an alternate, in-frame exon, compared to variant 1, resulting in a shorter protein (isoform 2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020115.1
CDS	264..1598
Translation	MASESGKLWGGRFVGAVDPIMEKFNASIAYDRHLWEVDVQGSKAYSRGLEKAGLLTKAEMDQILHGLDKVAEEWAQGTFKLNSNDEDIHTANERRLKELIGATAGKLHTGRSRNDQVVTDLRLWMRQTCSTLSGLLWELIRTMVDRAEAERDVLFPGYTHLQRAQPIRWSHWILSHAVALTRDSERLLEVRKRINVLPLGSGAIAGNPLGVDRELLRAELNFGAITLNSMDATSERDFVAEFLFWASLCMTHLSRMAEDLILYCTKEFSFVQLSDAYSTGSSLMPQKKNPDSLELIRSKAGRVFGREDKEAVFEVSDTMSAVLQVATGVISTLQIHQENMGQALSPDMLATDLAYYLVRKGMPFRQAHEASGKAVFMAETKGVALNQLSLQELQTISPLFSGDVICVWDYGHSVEQYGALGGTARSSVDWQIRQVRALLQAQQA

Target ORF information:

RefSeq Version	NM_001024944.2
Organism	Homo sapiens(human)
Definition	Homo sapiens argininosuccinate lyase (ASL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024944.2

ORF Insert Sequence:

ATGGCCTCGG AGAGTGGGAA GCTTTGGGGT GGCCGGTTTG TGGGTGCAGT GGACCCCATC 
ATGGAGAAGT TCAACGCGTC CATTGCCTAC GACCGGCACC TTTGGGAGGT GGATGTTCAA 
GGCAGCAAAG CCTACAGCAG GGGCCTGGAG AAGGCAGGGC TCCTCACCAA GGCCGAGATG 
GACCAGATAC TCCATGGCCT AGACAAGGTG GCTGAGGAGT GGGCCCAGGG CACCTTCAAA 
CTGAACTCCA ATGATGAGGA CATCCACACA GCCAATGAGC GCCGCCTGAA GGAGCTCATT 
GGTGCAACGG CAGGGAAGCT GCACACGGGA CGGAGCCGGA ATGACCAGGT GGTCACAGAC 
CTCAGGCTGT GGATGCGGCA GACCTGCTCC ACGCTCTCGG GCCTCCTCTG GGAGCTCATT 
AGGACCATGG TGGATCGGGC AGAGGCGGAA CGTGATGTTC TCTTCCCGGG GTACACCCAT 
TTGCAGAGGG CCCAGCCCAT CCGCTGGAGC CACTGGATTC TGAGCCACGC CGTGGCACTG 
ACCCGAGACT CTGAGCGGCT GCTGGAGGTG CGGAAGCGGA TCAATGTCCT GCCCCTGGGG 
AGTGGGGCCA TTGCAGGCAA TCCCCTGGGT GTGGACCGAG AGCTGCTCCG AGCAGAACTC 
AACTTTGGGG CCATCACTCT CAACAGCATG GATGCCACTA GTGAGCGGGA CTTTGTGGCC 
GAGTTCCTGT TCTGGGCTTC GCTGTGCATG ACCCATCTCA GCAGGATGGC CGAGGACCTC 
ATCCTCTACT GCACCAAGGA ATTCAGCTTC GTGCAGCTCT CAGATGCCTA CAGCACGGGA 
AGCAGCCTGA TGCCCCAGAA GAAAAACCCC GACAGTTTGG AGCTGATCCG GAGCAAGGCT 
GGGCGTGTGT TTGGGCGGGA GGACAAGGAA GCTGTGTTTG AAGTGTCAGA CACTATGAGT 
GCCGTGCTCC AGGTGGCCAC TGGCGTCATC TCTACGCTGC AGATTCACCA AGAGAACATG 
GGACAGGCTC TCAGCCCCGA CATGCTGGCC ACTGACCTTG CCTATTACCT GGTCCGCAAA 
GGGATGCCAT TCCGCCAGGC CCACGAGGCC TCCGGGAAAG CTGTGTTCAT GGCCGAGACC 
AAGGGGGTCG CCCTCAACCA GCTGTCACTG CAGGAGCTGC AGACCATCAG CCCCCTGTTC 
TCGGGCGACG TGATCTGCGT GTGGGACTAC GGGCACAGTG TGGAGCAGTA TGGTGCCCTG 
GGCGGCACTG CGCGCTCCAG CGTCGACTGG CAGATCCGCC AGGTGCGGGC GCTACTGCAG 
GCACAGCAGG CCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

[Mutational analysis of ASS1, ASL and SLC25A13 genes in six Chinese patients with citrullinemia].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(5)676-679(2017 Oct)
Lin Y,Yu K,Li L,Zheng Z,Lin W,Fu Q

Argininosuccinate lyase interacts with cyclin A2 in cytoplasm and modulates growth of liver tumor cells.
Oncology reports37(2)969-978(2017 Feb)
Hung YH,Huang HL,Chen WC,Yen MC,Cho CY,Weng TY,Wang CY,Chen YL,Chen LT,Lai MD

Silencing of argininosuccinate lyase inhibits colorectal cancer formation.
Oncology reports37(1)163-170(2017 Jan)
Huang HL,Chen WC,Hsu HP,Cho CY,Hung YH,Wang CY,Lai MD

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

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ASL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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