MMP1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MMP1
Entrez Gene ID	4312
Full Name	matrix metallopeptidase 1
Synonyms	CLG,CLGN
General protein information	Preferred Names matrix metallopeptidase 1 Names interstitial collagenase fibroblast collagenase matrix metallopeptidase 1 (interstitial collagenase) matrix metalloprotease 1 matrix metalloproteinase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q22.2
Summary	This gene encodes a member of the peptidase M10 family of matrix metalloproteinases (MMPs). Proteins in this family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. The encoded preproprotein is proteolytically processed to generate the mature protease. This secreted protease breaks down the interstitial collagens, including types I, II, and III. The gene is part of a cluster of MMP genes on chromosome 11. Mutations in this gene are associated with chronic obstructive pulmonary disease (COPD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Jan 2016].
Disorder MIM:	120353
Disorder Html:	COPD, rate of decline of lung function in, 606963 (3); {Epidermolysis bullosa dystrophica, autosomal recessive, modifier of}, 226600 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002421.3	NP_002412.1	interstitial collagenase isoform 1 preproprotein
NM_002421.4	NP_002412.1	interstitial collagenase isoform 1 preproprotein
NM_001145938.2	NP_001139410.1	interstitial collagenase isoform 2
NM_001145938.1	NP_001139410.1	interstitial collagenase isoform 2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MMP1	XP_508722.2
	MMP1	XP_001098302.1
Gallus gallus (chicken)	MMP1	XP_417176.1
Homo sapiens (human)	MMP1	NP_002412.1
Bos taurus (cattle)	MMP1	NP_776537.1
Mus musculus (house mouse)	Mmp1a	NP_114395.1
Bos taurus (cattle)	LOC100848085	XP_003586977.1
Rattus norvegicus (Norway rat)	Mmp1	NP_001128002.1
Canis lupus familiaris (dog)	LOC489428	XP_546546.3

Gene Ontology

The following MMP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MMP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23282	NM_002421.4 Latest version!	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu23518	NM_001145938.2 Latest version!	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu23282	NM_002421.3	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu23518	NM_001145938.1	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MMP1 ( NM_002421.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002412.1 Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 1, mRNA.

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CloneID	OHu23282
Clone ID Related Accession (Same CDS sequence)	NM_002421.3 , NM_002421.4
Accession Version	NM_002421.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-28
Organism	Homo sapiens(human)
Product	interstitial collagenase isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC385245.1, BC013875.2, AP000619.5 and AK223036.1. This sequence is a reference standard in the RefSeqGene project. On Mar 21, 2009 this sequence version replaced NM_002421.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK024818.1, BC013875.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACAGCT TTCCTCCACT GCTGCTGCTG CTGTTCTGGG GTGTGGTGTC TCACAGCTTC 
CCAGCGACTC TAGAAACACA AGAGCAAGAT GTGGACTTAG TCCAGAAATA CCTGGAAAAA 
TACTACAACC TGAAGAATGA TGGGAGGCAA GTTGAAAAGC GGAGAAATAG TGGCCCAGTG 
GTTGAAAAAT TGAAGCAAAT GCAGGAATTC TTTGGGCTGA AAGTGACTGG GAAACCAGAT 
GCTGAAACCC TGAAGGTGAT GAAGCAGCCC AGATGTGGAG TGCCTGATGT GGCTCAGTTT 
GTCCTCACTG AGGGGAACCC TCGCTGGGAG CAAACACATC TGACCTACAG GATTGAAAAT 
TACACGCCAG ATTTGCCAAG AGCAGATGTG GACCATGCCA TTGAGAAAGC CTTCCAACTC 
TGGAGTAATG TCACACCTCT GACATTCACC AAGGTCTCTG AGGGTCAAGC AGACATCATG 
ATATCTTTTG TCAGGGGAGA TCATCGGGAC AACTCTCCTT TTGATGGACC TGGAGGAAAT 
CTTGCTCATG CTTTTCAACC AGGCCCAGGT ATTGGAGGGG ATGCTCATTT TGATGAAGAT 
GAAAGGTGGA CCAACAATTT CAGAGAGTAC AACTTACATC GTGTTGCAGC TCATGAACTC 
GGCCATTCTC TTGGACTCTC CCATTCTACT GATATCGGGG CTTTGATGTA CCCTAGCTAC 
ACCTTCAGTG GTGATGTTCA GCTAGCTCAG GATGACATTG ATGGCATCCA AGCCATATAT 
GGACGTTCCC AAAATCCTGT CCAGCCCATC GGCCCACAAA CCCCAAAAGC GTGTGACAGT 
AAGCTAACCT TTGATGCTAT AACTACGATT CGGGGAGAAG TGATGTTCTT TAAAGACAGA 
TTCTACATGC GCACAAATCC CTTCTACCCG GAAGTTGAGC TCAATTTCAT TTCTGTTTTC 
TGGCCACAAC TGCCAAATGG GCTTGAAGCT GCTTACGAAT TTGCCGACAG AGATGAAGTC 
CGGTTTTTCA AAGGGAATAA GTACTGGGCT GTTCAGGGAC AGAATGTGCT ACACGGATAC 
CCCAAGGACA TCTACAGCTC CTTTGGCTTC CCTAGAACTG TGAAGCATAT CGATGCTGCT 
CTTTCTGAGG AAAACACTGG AAAAACCTAC TTCTTTGTTG CTAACAAATA CTGGAGGTAT 
GATGAATATA AACGATCTAT GGATCCAGGT TATCCCAAAA TGATAGCACA TGACTTTCCT 
GGAATTGGCC ACAAAGTTGA TGCAGTTTTC ATGAAAGATG GATTTTTCTA TTTCTTTCAT 
GGAACAAGAC AATACAAATT TGATCCTAAA ACGAAGAGAA TTTTGACTCT CCAGAAAGCT 
AATAGCTGGT TCAACTGCAG GAAAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002412.1
CDS	144..1553
Translation	MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

Target ORF information:

RefSeq Version	NM_002421.3
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002421.3

ORF Insert Sequence:

ATGCACAGCT TTCCTCCACT GCTGCTGCTG CTGTTCTGGG GTGTGGTGTC TCACAGCTTC 
CCAGCGACTC TAGAAACACA AGAGCAAGAT GTGGACTTAG TCCAGAAATA CCTGGAAAAA 
TACTACAACC TGAAGAATGA TGGGAGGCAA GTTGAAAAGC GGAGAAATAG TGGCCCAGTG 
GTTGAAAAAT TGAAGCAAAT GCAGGAATTC TTTGGGCTGA AAGTGACTGG GAAACCAGAT 
GCTGAAACCC TGAAGGTGAT GAAGCAGCCC AGATGTGGAG TGCCTGATGT GGCTCAGTTT 
GTCCTCACTG AGGGGAACCC TCGCTGGGAG CAAACACATC TGACCTACAG GATTGAAAAT 
TACACGCCAG ATTTGCCAAG AGCAGATGTG GACCATGCCA TTGAGAAAGC CTTCCAACTC 
TGGAGTAATG TCACACCTCT GACATTCACC AAGGTCTCTG AGGGTCAAGC AGACATCATG 
ATATCTTTTG TCAGGGGAGA TCATCGGGAC AACTCTCCTT TTGATGGACC TGGAGGAAAT 
CTTGCTCATG CTTTTCAACC AGGCCCAGGT ATTGGAGGGG ATGCTCATTT TGATGAAGAT 
GAAAGGTGGA CCAACAATTT CAGAGAGTAC AACTTACATC GTGTTGCAGC TCATGAACTC 
GGCCATTCTC TTGGACTCTC CCATTCTACT GATATCGGGG CTTTGATGTA CCCTAGCTAC 
ACCTTCAGTG GTGATGTTCA GCTAGCTCAG GATGACATTG ATGGCATCCA AGCCATATAT 
GGACGTTCCC AAAATCCTGT CCAGCCCATC GGCCCACAAA CCCCAAAAGC GTGTGACAGT 
AAGCTAACCT TTGATGCTAT AACTACGATT CGGGGAGAAG TGATGTTCTT TAAAGACAGA 
TTCTACATGC GCACAAATCC CTTCTACCCG GAAGTTGAGC TCAATTTCAT TTCTGTTTTC 
TGGCCACAAC TGCCAAATGG GCTTGAAGCT GCTTACGAAT TTGCCGACAG AGATGAAGTC 
CGGTTTTTCA AAGGGAATAA GTACTGGGCT GTTCAGGGAC AGAATGTGCT ACACGGATAC 
CCCAAGGACA TCTACAGCTC CTTTGGCTTC CCTAGAACTG TGAAGCATAT CGATGCTGCT 
CTTTCTGAGG AAAACACTGG AAAAACCTAC TTCTTTGTTG CTAACAAATA CTGGAGGTAT 
GATGAATATA AACGATCTAT GGATCCAGGT TATCCCAAAA TGATAGCACA TGACTTTCCT 
GGAATTGGCC ACAAAGTTGA TGCAGTTTTC ATGAAAGATG GATTTTTCTA TTTCTTTCAT 
GGAACAAGAC AATACAAATT TGATCCTAAA ACGAAGAGAA TTTTGACTCT CCAGAAAGCT 
AATAGCTGGT TCAACTGCAG GAAAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP1 ( NM_002421.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002412.1 Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23282
Clone ID Related Accession (Same CDS sequence)	NM_002421.3 , NM_002421.4
Accession Version	NM_002421.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	interstitial collagenase isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC385245.1, BC013875.2 and AP000619.5. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002421.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M13509.1, AK130687.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000315274.7/ ENSP00000322788.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCACAGCT TTCCTCCACT GCTGCTGCTG CTGTTCTGGG GTGTGGTGTC TCACAGCTTC 
CCAGCGACTC TAGAAACACA AGAGCAAGAT GTGGACTTAG TCCAGAAATA CCTGGAAAAA 
TACTACAACC TGAAGAATGA TGGGAGGCAA GTTGAAAAGC GGAGAAATAG TGGCCCAGTG 
GTTGAAAAAT TGAAGCAAAT GCAGGAATTC TTTGGGCTGA AAGTGACTGG GAAACCAGAT 
GCTGAAACCC TGAAGGTGAT GAAGCAGCCC AGATGTGGAG TGCCTGATGT GGCTCAGTTT 
GTCCTCACTG AGGGGAACCC TCGCTGGGAG CAAACACATC TGACCTACAG GATTGAAAAT 
TACACGCCAG ATTTGCCAAG AGCAGATGTG GACCATGCCA TTGAGAAAGC CTTCCAACTC 
TGGAGTAATG TCACACCTCT GACATTCACC AAGGTCTCTG AGGGTCAAGC AGACATCATG 
ATATCTTTTG TCAGGGGAGA TCATCGGGAC AACTCTCCTT TTGATGGACC TGGAGGAAAT 
CTTGCTCATG CTTTTCAACC AGGCCCAGGT ATTGGAGGGG ATGCTCATTT TGATGAAGAT 
GAAAGGTGGA CCAACAATTT CAGAGAGTAC AACTTACATC GTGTTGCAGC TCATGAACTC 
GGCCATTCTC TTGGACTCTC CCATTCTACT GATATCGGGG CTTTGATGTA CCCTAGCTAC 
ACCTTCAGTG GTGATGTTCA GCTAGCTCAG GATGACATTG ATGGCATCCA AGCCATATAT 
GGACGTTCCC AAAATCCTGT CCAGCCCATC GGCCCACAAA CCCCAAAAGC GTGTGACAGT 
AAGCTAACCT TTGATGCTAT AACTACGATT CGGGGAGAAG TGATGTTCTT TAAAGACAGA 
TTCTACATGC GCACAAATCC CTTCTACCCG GAAGTTGAGC TCAATTTCAT TTCTGTTTTC 
TGGCCACAAC TGCCAAATGG GCTTGAAGCT GCTTACGAAT TTGCCGACAG AGATGAAGTC 
CGGTTTTTCA AAGGGAATAA GTACTGGGCT GTTCAGGGAC AGAATGTGCT ACACGGATAC 
CCCAAGGACA TCTACAGCTC CTTTGGCTTC CCTAGAACTG TGAAGCATAT CGATGCTGCT 
CTTTCTGAGG AAAACACTGG AAAAACCTAC TTCTTTGTTG CTAACAAATA CTGGAGGTAT 
GATGAATATA AACGATCTAT GGATCCAGGT TATCCCAAAA TGATAGCACA TGACTTTCCT 
GGAATTGGCC ACAAAGTTGA TGCAGTTTTC ATGAAAGATG GATTTTTCTA TTTCTTTCAT 
GGAACAAGAC AATACAAATT TGATCCTAAA ACGAAGAGAA TTTTGACTCT CCAGAAAGCT 
AATAGCTGGT TCAACTGCAG GAAAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002412.1
CDS	69..1478
Translation	MHSFPPLLLLLFWGVVSHSFPATLETQEQDVDLVQKYLEKYYNLKNDGRQVEKRRNSGPVVEKLKQMQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

Target ORF information:

RefSeq Version	NM_002421.4
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002421.4

ORF Insert Sequence:

ATGCACAGCT TTCCTCCACT GCTGCTGCTG CTGTTCTGGG GTGTGGTGTC TCACAGCTTC 
CCAGCGACTC TAGAAACACA AGAGCAAGAT GTGGACTTAG TCCAGAAATA CCTGGAAAAA 
TACTACAACC TGAAGAATGA TGGGAGGCAA GTTGAAAAGC GGAGAAATAG TGGCCCAGTG 
GTTGAAAAAT TGAAGCAAAT GCAGGAATTC TTTGGGCTGA AAGTGACTGG GAAACCAGAT 
GCTGAAACCC TGAAGGTGAT GAAGCAGCCC AGATGTGGAG TGCCTGATGT GGCTCAGTTT 
GTCCTCACTG AGGGGAACCC TCGCTGGGAG CAAACACATC TGACCTACAG GATTGAAAAT 
TACACGCCAG ATTTGCCAAG AGCAGATGTG GACCATGCCA TTGAGAAAGC CTTCCAACTC 
TGGAGTAATG TCACACCTCT GACATTCACC AAGGTCTCTG AGGGTCAAGC AGACATCATG 
ATATCTTTTG TCAGGGGAGA TCATCGGGAC AACTCTCCTT TTGATGGACC TGGAGGAAAT 
CTTGCTCATG CTTTTCAACC AGGCCCAGGT ATTGGAGGGG ATGCTCATTT TGATGAAGAT 
GAAAGGTGGA CCAACAATTT CAGAGAGTAC AACTTACATC GTGTTGCAGC TCATGAACTC 
GGCCATTCTC TTGGACTCTC CCATTCTACT GATATCGGGG CTTTGATGTA CCCTAGCTAC 
ACCTTCAGTG GTGATGTTCA GCTAGCTCAG GATGACATTG ATGGCATCCA AGCCATATAT 
GGACGTTCCC AAAATCCTGT CCAGCCCATC GGCCCACAAA CCCCAAAAGC GTGTGACAGT 
AAGCTAACCT TTGATGCTAT AACTACGATT CGGGGAGAAG TGATGTTCTT TAAAGACAGA 
TTCTACATGC GCACAAATCC CTTCTACCCG GAAGTTGAGC TCAATTTCAT TTCTGTTTTC 
TGGCCACAAC TGCCAAATGG GCTTGAAGCT GCTTACGAAT TTGCCGACAG AGATGAAGTC 
CGGTTTTTCA AAGGGAATAA GTACTGGGCT GTTCAGGGAC AGAATGTGCT ACACGGATAC 
CCCAAGGACA TCTACAGCTC CTTTGGCTTC CCTAGAACTG TGAAGCATAT CGATGCTGCT 
CTTTCTGAGG AAAACACTGG AAAAACCTAC TTCTTTGTTG CTAACAAATA CTGGAGGTAT 
GATGAATATA AACGATCTAT GGATCCAGGT TATCCCAAAA TGATAGCACA TGACTTTCCT 
GGAATTGGCC ACAAAGTTGA TGCAGTTTTC ATGAAAGATG GATTTTTCTA TTTCTTTCAT 
GGAACAAGAC AATACAAATT TGATCCTAAA ACGAAGAGAA TTTTGACTCT CCAGAAAGCT 
AATAGCTGGT TCAACTGCAG GAAAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP1 ( NM_001145938.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139410.1 Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23518
Clone ID Related Accession (Same CDS sequence)	NM_001145938.2 , NM_001145938.1
Accession Version	NM_001145938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	interstitial collagenase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000619.5 and AK297723.1. On May 31, 2019 this sequence version replaced NM_001145938.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297723.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGAAT TCTTTGGGCT GAAAGTGACT GGGAAACCAG ATGCTGAAAC CCTGAAGGTG 
ATGAAGCAGC CCAGATGTGG AGTGCCTGAT GTGGCTCAGT TTGTCCTCAC TGAGGGGAAC 
CCTCGCTGGG AGCAAACACA TCTGACCTAC AGGATTGAAA ATTACACGCC AGATTTGCCA 
AGAGCAGATG TGGACCATGC CATTGAGAAA GCCTTCCAAC TCTGGAGTAA TGTCACACCT 
CTGACATTCA CCAAGGTCTC TGAGGGTCAA GCAGACATCA TGATATCTTT TGTCAGGGGA 
GATCATCGGG ACAACTCTCC TTTTGATGGA CCTGGAGGAA ATCTTGCTCA TGCTTTTCAA 
CCAGGCCCAG GTATTGGAGG GGATGCTCAT TTTGATGAAG ATGAAAGGTG GACCAACAAT 
TTCAGAGAGT ACAACTTACA TCGTGTTGCA GCTCATGAAC TCGGCCATTC TCTTGGACTC 
TCCCATTCTA CTGATATCGG GGCTTTGATG TACCCTAGCT ACACCTTCAG TGGTGATGTT 
CAGCTAGCTC AGGATGACAT TGATGGCATC CAAGCCATAT ATGGACGTTC CCAAAATCCT 
GTCCAGCCCA TCGGCCCACA AACCCCAAAA GCGTGTGACA GTAAGCTAAC CTTTGATGCT 
ATAACTACGA TTCGGGGAGA AGTGATGTTC TTTAAAGACA GATTCTACAT GCGCACAAAT 
CCCTTCTACC CGGAAGTTGA GCTCAATTTC ATTTCTGTTT TCTGGCCACA ACTGCCAAAT 
GGGCTTGAAG CTGCTTACGA ATTTGCCGAC AGAGATGAAG TCCGGTTTTT CAAAGGGAAT 
AAGTACTGGG CTGTTCAGGG ACAGAATGTG CTACACGGAT ACCCCAAGGA CATCTACAGC 
TCCTTTGGCT TCCCTAGAAC TGTGAAGCAT ATCGATGCTG CTCTTTCTGA GGAAAACACT 
GGAAAAACCT ACTTCTTTGT TGCTAACAAA TACTGGAGGT ATGATGAATA TAAACGATCT 
ATGGATCCAG GTTATCCCAA AATGATAGCA CATGACTTTC CTGGAATTGG CCACAAAGTT 
GATGCAGTTT TCATGAAAGA TGGATTTTTC TATTTCTTTC ATGGAACAAG ACAATACAAA 
TTTGATCCTA AAACGAAGAG AATTTTGACT CTCCAGAAAG CTAATAGCTG GTTCAACTGC 
AGGAAAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139410.1
CDS	115..1326
Translation	MQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

Target ORF information:

RefSeq Version	NM_001145938.2
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145938.2

ORF Insert Sequence:

ATGCAGGAAT TCTTTGGGCT GAAAGTGACT GGGAAACCAG ATGCTGAAAC CCTGAAGGTG 
ATGAAGCAGC CCAGATGTGG AGTGCCTGAT GTGGCTCAGT TTGTCCTCAC TGAGGGGAAC 
CCTCGCTGGG AGCAAACACA TCTGACCTAC AGGATTGAAA ATTACACGCC AGATTTGCCA 
AGAGCAGATG TGGACCATGC CATTGAGAAA GCCTTCCAAC TCTGGAGTAA TGTCACACCT 
CTGACATTCA CCAAGGTCTC TGAGGGTCAA GCAGACATCA TGATATCTTT TGTCAGGGGA 
GATCATCGGG ACAACTCTCC TTTTGATGGA CCTGGAGGAA ATCTTGCTCA TGCTTTTCAA 
CCAGGCCCAG GTATTGGAGG GGATGCTCAT TTTGATGAAG ATGAAAGGTG GACCAACAAT 
TTCAGAGAGT ACAACTTACA TCGTGTTGCA GCTCATGAAC TCGGCCATTC TCTTGGACTC 
TCCCATTCTA CTGATATCGG GGCTTTGATG TACCCTAGCT ACACCTTCAG TGGTGATGTT 
CAGCTAGCTC AGGATGACAT TGATGGCATC CAAGCCATAT ATGGACGTTC CCAAAATCCT 
GTCCAGCCCA TCGGCCCACA AACCCCAAAA GCGTGTGACA GTAAGCTAAC CTTTGATGCT 
ATAACTACGA TTCGGGGAGA AGTGATGTTC TTTAAAGACA GATTCTACAT GCGCACAAAT 
CCCTTCTACC CGGAAGTTGA GCTCAATTTC ATTTCTGTTT TCTGGCCACA ACTGCCAAAT 
GGGCTTGAAG CTGCTTACGA ATTTGCCGAC AGAGATGAAG TCCGGTTTTT CAAAGGGAAT 
AAGTACTGGG CTGTTCAGGG ACAGAATGTG CTACACGGAT ACCCCAAGGA CATCTACAGC 
TCCTTTGGCT TCCCTAGAAC TGTGAAGCAT ATCGATGCTG CTCTTTCTGA GGAAAACACT 
GGAAAAACCT ACTTCTTTGT TGCTAACAAA TACTGGAGGT ATGATGAATA TAAACGATCT 
ATGGATCCAG GTTATCCCAA AATGATAGCA CATGACTTTC CTGGAATTGG CCACAAAGTT 
GATGCAGTTT TCATGAAAGA TGGATTTTTC TATTTCTTTC ATGGAACAAG ACAATACAAA 
TTTGATCCTA AAACGAAGAG AATTTTGACT CTCCAGAAAG CTAATAGCTG GTTCAACTGC 
AGGAAAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MMP1 ( NM_001145938.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001139410.1 Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 2, mRNA.

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CloneID	OHu23518
Clone ID Related Accession (Same CDS sequence)	NM_001145938.2 , NM_001145938.1
Accession Version	NM_001145938.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	interstitial collagenase isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AP000619.5 and AK297723.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region and uses a downstream start codon compared to variant 1. The resulting protein (isoform 2) has a shorter N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297723.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGGAAT TCTTTGGGCT GAAAGTGACT GGGAAACCAG ATGCTGAAAC CCTGAAGGTG 
ATGAAGCAGC CCAGATGTGG AGTGCCTGAT GTGGCTCAGT TTGTCCTCAC TGAGGGGAAC 
CCTCGCTGGG AGCAAACACA TCTGACCTAC AGGATTGAAA ATTACACGCC AGATTTGCCA 
AGAGCAGATG TGGACCATGC CATTGAGAAA GCCTTCCAAC TCTGGAGTAA TGTCACACCT 
CTGACATTCA CCAAGGTCTC TGAGGGTCAA GCAGACATCA TGATATCTTT TGTCAGGGGA 
GATCATCGGG ACAACTCTCC TTTTGATGGA CCTGGAGGAA ATCTTGCTCA TGCTTTTCAA 
CCAGGCCCAG GTATTGGAGG GGATGCTCAT TTTGATGAAG ATGAAAGGTG GACCAACAAT 
TTCAGAGAGT ACAACTTACA TCGTGTTGCA GCTCATGAAC TCGGCCATTC TCTTGGACTC 
TCCCATTCTA CTGATATCGG GGCTTTGATG TACCCTAGCT ACACCTTCAG TGGTGATGTT 
CAGCTAGCTC AGGATGACAT TGATGGCATC CAAGCCATAT ATGGACGTTC CCAAAATCCT 
GTCCAGCCCA TCGGCCCACA AACCCCAAAA GCGTGTGACA GTAAGCTAAC CTTTGATGCT 
ATAACTACGA TTCGGGGAGA AGTGATGTTC TTTAAAGACA GATTCTACAT GCGCACAAAT 
CCCTTCTACC CGGAAGTTGA GCTCAATTTC ATTTCTGTTT TCTGGCCACA ACTGCCAAAT 
GGGCTTGAAG CTGCTTACGA ATTTGCCGAC AGAGATGAAG TCCGGTTTTT CAAAGGGAAT 
AAGTACTGGG CTGTTCAGGG ACAGAATGTG CTACACGGAT ACCCCAAGGA CATCTACAGC 
TCCTTTGGCT TCCCTAGAAC TGTGAAGCAT ATCGATGCTG CTCTTTCTGA GGAAAACACT 
GGAAAAACCT ACTTCTTTGT TGCTAACAAA TACTGGAGGT ATGATGAATA TAAACGATCT 
ATGGATCCAG GTTATCCCAA AATGATAGCA CATGACTTTC CTGGAATTGG CCACAAAGTT 
GATGCAGTTT TCATGAAAGA TGGATTTTTC TATTTCTTTC ATGGAACAAG ACAATACAAA 
TTTGATCCTA AAACGAAGAG AATTTTGACT CTCCAGAAAG CTAATAGCTG GTTCAACTGC 
AGGAAAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001139410.1
CDS	190..1401
Translation	MQEFFGLKVTGKPDAETLKVMKQPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLTFTKVSEGQADIMISFVRGDHRDNSPFDGPGGNLAHAFQPGPGIGGDAHFDEDERWTNNFREYNLHRVAAHELGHSLGLSHSTDIGALMYPSYTFSGDVQLAQDDIDGIQAIYGRSQNPVQPIGPQTPKACDSKLTFDAITTIRGEVMFFKDRFYMRTNPFYPEVELNFISVFWPQLPNGLEAAYEFADRDEVRFFKGNKYWAVQGQNVLHGYPKDIYSSFGFPRTVKHIDAALSEENTGKTYFFVANKYWRYDEYKRSMDPGYPKMIAHDFPGIGHKVDAVFMKDGFFYFFHGTRQYKFDPKTKRILTLQKANSWFNCRKN

Target ORF information:

RefSeq Version	NM_001145938.1
Organism	Homo sapiens(human)
Definition	Homo sapiens matrix metallopeptidase 1 (MMP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145938.1

ORF Insert Sequence:

ATGCAGGAAT TCTTTGGGCT GAAAGTGACT GGGAAACCAG ATGCTGAAAC CCTGAAGGTG 
ATGAAGCAGC CCAGATGTGG AGTGCCTGAT GTGGCTCAGT TTGTCCTCAC TGAGGGGAAC 
CCTCGCTGGG AGCAAACACA TCTGACCTAC AGGATTGAAA ATTACACGCC AGATTTGCCA 
AGAGCAGATG TGGACCATGC CATTGAGAAA GCCTTCCAAC TCTGGAGTAA TGTCACACCT 
CTGACATTCA CCAAGGTCTC TGAGGGTCAA GCAGACATCA TGATATCTTT TGTCAGGGGA 
GATCATCGGG ACAACTCTCC TTTTGATGGA CCTGGAGGAA ATCTTGCTCA TGCTTTTCAA 
CCAGGCCCAG GTATTGGAGG GGATGCTCAT TTTGATGAAG ATGAAAGGTG GACCAACAAT 
TTCAGAGAGT ACAACTTACA TCGTGTTGCA GCTCATGAAC TCGGCCATTC TCTTGGACTC 
TCCCATTCTA CTGATATCGG GGCTTTGATG TACCCTAGCT ACACCTTCAG TGGTGATGTT 
CAGCTAGCTC AGGATGACAT TGATGGCATC CAAGCCATAT ATGGACGTTC CCAAAATCCT 
GTCCAGCCCA TCGGCCCACA AACCCCAAAA GCGTGTGACA GTAAGCTAAC CTTTGATGCT 
ATAACTACGA TTCGGGGAGA AGTGATGTTC TTTAAAGACA GATTCTACAT GCGCACAAAT 
CCCTTCTACC CGGAAGTTGA GCTCAATTTC ATTTCTGTTT TCTGGCCACA ACTGCCAAAT 
GGGCTTGAAG CTGCTTACGA ATTTGCCGAC AGAGATGAAG TCCGGTTTTT CAAAGGGAAT 
AAGTACTGGG CTGTTCAGGG ACAGAATGTG CTACACGGAT ACCCCAAGGA CATCTACAGC 
TCCTTTGGCT TCCCTAGAAC TGTGAAGCAT ATCGATGCTG CTCTTTCTGA GGAAAACACT 
GGAAAAACCT ACTTCTTTGT TGCTAACAAA TACTGGAGGT ATGATGAATA TAAACGATCT 
ATGGATCCAG GTTATCCCAA AATGATAGCA CATGACTTTC CTGGAATTGG CCACAAAGTT 
GATGCAGTTT TCATGAAAGA TGGATTTTTC TATTTCTTTC ATGGAACAAG ACAATACAAA 
TTTGATCCTA AAACGAAGAG AATTTTGACT CTCCAGAAAG CTAATAGCTG GTTCAACTGC 
AGGAAAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MMP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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