TRPM1 cDNA ORF clone, Homo sapiens(human)

The following TRPM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRPM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26661 NM_001252020.1
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$579.50-$811.30
$1159.00
OHu14795 NM_001252030.2
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu14262 NM_001252024.2
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu20829 NM_002420.6
Latest version!
Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
View Old Accession Versions >>
OHu20829 NM_002420.5 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $923.30
$1319.00
OHu14262 NM_001252024.1 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu14795 NM_001252030.1 Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26661
    Clone ID Related Accession (Same CDS sequence) NM_001252020.1
    Accession Version NM_001252020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4929bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 1 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HM135791.1 and BU743036.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM135791.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGCTCCT TCAAGCGGGG CTCGCTCAAG AGCTCCACAT CAGGGTCGCA GAAGGGTCAG 
    AAATCTTGGA TAGAGAAAAC CTTTTGCAAA CGGGAATGTA TCTTTGTAAT TCCTAGCATG
    AAAGACTCTA ACAGGTGTTG CTGTGGCCAG TTCACCAACC AGCATATCCC CCCTCTGCCA
    AGTGCAACAC CCAGCAAAAA TGAAGAGGAA AGCAAACAGG TGGAGACTCA GCCTGAGAAA
    TGGTCTGTTG CCAAGCACAC CCAGAGCTAC CCAACAGATT CCTATGGAGT TCTTGAATTC
    CAGGGTGGCG GATATTCCAA TAAAGCCATG TATATCCGTG TATCCTATGA CACCAAGCCA
    GACTCACTGC TCCATCTCAT GGTGAAAGAT TGGCAGCTGG AACTCCCCAA GCTCTTAATA
    TCTGTGCATG GAGGCCTCCA GAACTTTGAG ATGCAGCCCA AGCTGAAACA AGTCTTTGGG
    AAAGGCCTGA TCAAGGCTGC TATGACCACC GGGGCCTGGA TCTTCACCGG GGGTGTCAGC
    ACAGGTGTTA TCAGCCACGT AGGGGATGCC TTGAAAGACC ACTCCTCCAA GTCCAGAGGC
    CGGGTTTGTG CTATAGGAAT TGCTCCATGG GGCATCGTGG AGAATAAGGA AGACCTGGTT
    GGAAAGGATG TAACAAGAGT GTACCAGACC ATGTCCAACC CTCTAAGTAA GCTCTCTGTG
    CTCAACAACT CCCACACCCA CTTCATCCTG GCTGACAATG GCACCCTGGG CAAGTATGGC
    GCCGAGGTGA AGCTGCGAAG GCTGCTGGAA AAGCACATCT CCCTGCAGAA GATCAACACA
    AGACTGGGGC AGGGCGTGCC CCTCGTGGGT CTCGTGGTGG AGGGGGGCCC TAACGTGGTG
    TCCATCGTCT TGGAATACCT GCAAGAAGAG CCTCCCATCC CTGTGGTGAT TTGTGATGGC
    AGCGGACGTG CCTCGGACAT CCTGTCCTTT GCGCACAAGT ACTGTGAAGA AGGCGGAATA
    ATAAATGAGT CCCTCAGGGA GCAGCTTCTA GTTACCATTC AGAAAACATT TAATTATAAT
    AAGGCACAAT CACATCAGCT GTTTGCAATT ATAATGGAGT GCATGAAGAA GAAAGAACTC
    GTCACTGTGT TCAGAATGGG TTCTGAGGGC CAGCAGGACA TCGAGATGGC AATTTTAACT
    GCCCTGCTGA AAGGAACAAA CGTATCTGCT CCAGATCAGC TGAGCTTGGC ACTGGCTTGG
    AACCGCGTGG ACATAGCACG AAGCCAGATC TTTGTCTTTG GGCCCCACTG GCCGCCCCTG
    GGAAGCCTGG CACCCCCGAC GGACAGCAAA GCCACGGAGA AGGAGAAGAA GCCACCCATG
    GCCACCACCA AGGGAGGAAG AGGAAAAGGG AAAGGCAAGA AGAAAGGGAA AGTGAAAGAG
    GAAGTGGAGG AAGAAACTGA CCCCCGGAAG ATAGAGCTGC TGAACTGGGT GAATGCTTTG
    GAGCAAGCGA TGCTAGATGC TTTAGTCTTA GATCGTGTCG ACTTTGTGAA GCTCCTGATT
    GAAAACGGAG TGAACATGCA ACACTTTCTG ACCATTCCGA GGCTGGAGGA GCTTTATAAC
    ACAAGACTGG GTCCACCAAA CACACTTCAT CTGCTGGTGA GGGATGTGAA AAAGAGCAAC
    CTTCCGCCTG ATTACCACAT CAGCCTCATA GACATCGGGC TCGTGCTGGA GTACCTCATG
    GGAGGAGCCT ACCGCTGCAA CTACACTCGG AAAAACTTTC GGACCCTTTA CAACAACTTG
    TTTGGACCAA AGAGGCCTAA AGCTCTTAAA CTTCTGGGAA TGGAAGATGA TGAGCCTCCA
    GCTAAAGGGA AGAAAAAGAA AAAGAAGAAA AAGGAGGAAG AGATCGACAT TGATGTGGAC
    GACCCTGCCG TGAGTCGGTT CCAGTATCCC TTCCACGAGC TGATGGTGTG GGCAGTGCTG
    ATGAAACGCC AGAAAATGGC AGTGTTCCTC TGGCAGCGAG GGGAAGAGAG CATGGCCAAG
    GCCCTGGTGG CCTGCAAGCT CTACAAGGCC ATGGCCCACG AGTCCTCCGA GAGTGATCTG
    GTGGATGACA TCTCCCAGGA CTTGGATAAC AATTCCAAAG ACTTCGGCCA GCTTGCTTTG
    GAGTTATTAG ACCAGTCCTA TAAGCATGAC GAGCAGATCG CTATGAAACT CCTGACCTAC
    GAGCTGAAAA ACTGGAGCAA CTCGACCTGC CTCAAACTGG CCGTGGCAGC CAAACACCGG
    GACTTCATTG CTCACACCTG CAGCCAGATG CTGCTGACCG ATATGTGGAT GGGAAGACTG
    CGGATGCGGA AGAACCCCGG CCTGAAGGTT ATCATGGGGA TTCTTCTACC CCCCACCATC
    TTGTTTTTGG AATTTCGCAC ATATGATGAT TTCTCGTATC AAACATCCAA GGAAAATGAG
    GATGGCAAAG AAAAAGAAGA GGAAAATACG GATGCAAATG CAGATGCTGG CTCAAGAAAG
    GGGGATGAGG AGAACGAGCA CAAAAAACAG AGAAGTATTC CCATCGGAAC AAAGATCTGT
    GAATTCTATA ACGCGCCCAT TGTCAAGTTC TGGTTTTACA CAATATCATA CTTGGGCTAC
    CTGCTGCTGT TTAACTACGT CATCCTGGTG CGGATGGATG GCTGGCCGTC CCTCCAGGAG
    TGGATCGTCA TCTCCTACAT CGTGAGCCTG GCGTTAGAGA AGATACGAGA GATCCTCATG
    TCAGAACCAG GCAAACTCAG CCAGAAAATC AAAGTTTGGC TTCAGGAGTA CTGGAACATC
    ACAGATCTCG TGGCCATTTC CACATTCATG ATTGGAGCAA TTCTTCGCCT ACAGAACCAG
    CCCTACATGG GCTATGGCCG GGTGATCTAC TGTGTGGATA TCATCTTCTG GTACATCCGT
    GTCCTGGACA TCTTTGGTGT CAACAAGTAT CTGGGGCCAT ACGTGATGAT GATTGGAAAG
    ATGATGATCG ACATGCTGTA CTTTGTGGTC ATCATGCTGG TCGTGCTCAT GAGTTTCGGA
    GTAGCCCGTC AAGCCATTCT GCATCCAGAG GAGAAGCCCT CTTGGAAACT GGCCCGAAAC
    ATCTTCTACA TGCCCTACTG GATGATCTAT GGAGAGGTGT TTGCAGACCA GATAGACCTC
    TACGCCATGG AAATTAATCC TCCTTGTGGT GAGAACCTAT ATGATGAGGA GGGCAAGCGG
    CTTCCTCCCT GTATCCCCGG CGCCTGGCTC ACTCCAGCAC TCATGGCGTG CTATCTACTG
    GTCGCCAACA TCCTGCTGGT GAACCTGCTG ATTGCTGTGT TCAACAATAC CTTCTTTGAA
    GTAAAATCAA TATCCAACCA GGTGTGGAAG TTCCAGCGAT ATCAGCTGAT TATGACATTT
    CATGACAGGC CAGTCCTGCC CCCACCGATG ATCATTTTAA GCCACATCTA CATCATCATT
    ATGCGTCTCA GCGGCCGCTG CAGGAAAAAG AGAGAAGGGG ACCAAGAGGA ACGGGATCGT
    GGATTGAAGC TCTTCCTTAG CGACGAGGAG CTAAAGAGGC TGCATGAGTT CGAGGAGCAG
    TGCGTGCAGG AGCACTTCCG GGAGAAGGAG GATGAGCAGC AGTCGTCCAG CGACGAGCGC
    ATCCGGGTCA CTTCTGAAAG AGTTGAAAAT ATGTCAATGA GGTTGGAAGA AATCAATGAA
    AGAGAAACTT TTATGAAAAC TTCCCTGCAG ACTGTTGACC TTCGACTTGC TCAGCTAGAA
    GAATTATCTA ACAGAATGGT GAATGCTCTT GAAAATCTTG CGGGAATCGA CAGGTCTGAC
    CTGATCCAGG CACGGTCCCG GGCTTCTTCT GAATGTGAGG CAACGTATCT TCTCCGGCAA
    AGCAGCATCA ATAGCGCTGA TGGCTACAGC TTGTATCGAT ATCATTTTAA CGGAGAAGAG
    TTATTATTTG AGGATACATC TCTCTCCACG TCACCAGGGA CAGGAGTCAG GAAAAAAACC
    TGTTCCTTCC GTATAAAGGA AGAGAAGGAC GTGAAAACGC ACCTAGTCCC AGAATGTCAG
    AACAGTCTTC ACCTTTCACT GGGCACAAGC ACATCAGCAA CCCCAGATGG CAGTCACCTT
    GCAGTAGATG ACTTAAAGAA CGCTGAAGAG TCAAAATTAG GTCCAGATAT TGGGATTTCA
    AAGGAAGATG ATGAAAGACA GACAGACTCT AAAAAAGAAG AAACTATTTC CCCAAGTTTA
    AATAAAACAG ATGTGATACA TGGACAGGAC AAATCAGATG TTCAAAACAC TCAGCTAACA
    GTGGAAACGA CAAATATAGA AGGCACTATT TCCTATCCCC TGGAAGAAAC CAAAATTACA
    CGCTATTTCC CCGATGAAAC GATCAATGCT TGTAAAACAA TGAAGTCCAG AAGCTTCGTC
    TATTCCCGGG GAAGAAAGCT GGTCGGTGGG GTTAACCAGG ATGTAGAGTA CAGTTCAATC
    ACGGACCAGC AATTGACGAC GGAATGGCAA TGCCAAGTTC AAAAGATCAC GCGCTCTCAT
    AGCACAGATA TTCCTTACAT TGTGTCGGAA GCTGCAGTGC AAGCTGAGCA TAAAGAGCAG
    TTTGCAGATA TGCAAGATGA ACACCATGTC GCTGAAGCAA TTCCTCGAAT CCCTCGCTTG
    TCCCTAACCA TTACTGACAG AAATGGGATG GAAAACTTAC TGTCTGTGAA GCCAGATCAA
    ACTTTGGGAT TCCCATCTCT CAGGTCAAAA AGTTTACATG GACATCCTAG GAATGTGAAA
    TCCATTCAGG GAAAGTTAGA CAGATCTGGA CATGCCAGTA GTGTAAGCAG CTTAGTAATT
    GTGTCTGGAA TGACAGCAGA AGAAAAAAAG GTTAAGAAAG AGAAAGCTTC CACAGAAACT
    GAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001238949.1
    CDS315..5243
    Translation

    Target ORF information:

    RefSeq Version NM_001252020.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGAGCTCCT TCAAGCGGGG CTCGCTCAAG AGCTCCACAT CAGGGTCGCA GAAGGGTCAG 
    AAATCTTGGA TAGAGAAAAC CTTTTGCAAA CGGGAATGTA TCTTTGTAAT TCCTAGCATG
    AAAGACTCTA ACAGGTGTTG CTGTGGCCAG TTCACCAACC AGCATATCCC CCCTCTGCCA
    AGTGCAACAC CCAGCAAAAA TGAAGAGGAA AGCAAACAGG TGGAGACTCA GCCTGAGAAA
    TGGTCTGTTG CCAAGCACAC CCAGAGCTAC CCAACAGATT CCTATGGAGT TCTTGAATTC
    CAGGGTGGCG GATATTCCAA TAAAGCCATG TATATCCGTG TATCCTATGA CACCAAGCCA
    GACTCACTGC TCCATCTCAT GGTGAAAGAT TGGCAGCTGG AACTCCCCAA GCTCTTAATA
    TCTGTGCATG GAGGCCTCCA GAACTTTGAG ATGCAGCCCA AGCTGAAACA AGTCTTTGGG
    AAAGGCCTGA TCAAGGCTGC TATGACCACC GGGGCCTGGA TCTTCACCGG GGGTGTCAGC
    ACAGGTGTTA TCAGCCACGT AGGGGATGCC TTGAAAGACC ACTCCTCCAA GTCCAGAGGC
    CGGGTTTGTG CTATAGGAAT TGCTCCATGG GGCATCGTGG AGAATAAGGA AGACCTGGTT
    GGAAAGGATG TAACAAGAGT GTACCAGACC ATGTCCAACC CTCTAAGTAA GCTCTCTGTG
    CTCAACAACT CCCACACCCA CTTCATCCTG GCTGACAATG GCACCCTGGG CAAGTATGGC
    GCCGAGGTGA AGCTGCGAAG GCTGCTGGAA AAGCACATCT CCCTGCAGAA GATCAACACA
    AGACTGGGGC AGGGCGTGCC CCTCGTGGGT CTCGTGGTGG AGGGGGGCCC TAACGTGGTG
    TCCATCGTCT TGGAATACCT GCAAGAAGAG CCTCCCATCC CTGTGGTGAT TTGTGATGGC
    AGCGGACGTG CCTCGGACAT CCTGTCCTTT GCGCACAAGT ACTGTGAAGA AGGCGGAATA
    ATAAATGAGT CCCTCAGGGA GCAGCTTCTA GTTACCATTC AGAAAACATT TAATTATAAT
    AAGGCACAAT CACATCAGCT GTTTGCAATT ATAATGGAGT GCATGAAGAA GAAAGAACTC
    GTCACTGTGT TCAGAATGGG TTCTGAGGGC CAGCAGGACA TCGAGATGGC AATTTTAACT
    GCCCTGCTGA AAGGAACAAA CGTATCTGCT CCAGATCAGC TGAGCTTGGC ACTGGCTTGG
    AACCGCGTGG ACATAGCACG AAGCCAGATC TTTGTCTTTG GGCCCCACTG GCCGCCCCTG
    GGAAGCCTGG CACCCCCGAC GGACAGCAAA GCCACGGAGA AGGAGAAGAA GCCACCCATG
    GCCACCACCA AGGGAGGAAG AGGAAAAGGG AAAGGCAAGA AGAAAGGGAA AGTGAAAGAG
    GAAGTGGAGG AAGAAACTGA CCCCCGGAAG ATAGAGCTGC TGAACTGGGT GAATGCTTTG
    GAGCAAGCGA TGCTAGATGC TTTAGTCTTA GATCGTGTCG ACTTTGTGAA GCTCCTGATT
    GAAAACGGAG TGAACATGCA ACACTTTCTG ACCATTCCGA GGCTGGAGGA GCTTTATAAC
    ACAAGACTGG GTCCACCAAA CACACTTCAT CTGCTGGTGA GGGATGTGAA AAAGAGCAAC
    CTTCCGCCTG ATTACCACAT CAGCCTCATA GACATCGGGC TCGTGCTGGA GTACCTCATG
    GGAGGAGCCT ACCGCTGCAA CTACACTCGG AAAAACTTTC GGACCCTTTA CAACAACTTG
    TTTGGACCAA AGAGGCCTAA AGCTCTTAAA CTTCTGGGAA TGGAAGATGA TGAGCCTCCA
    GCTAAAGGGA AGAAAAAGAA AAAGAAGAAA AAGGAGGAAG AGATCGACAT TGATGTGGAC
    GACCCTGCCG TGAGTCGGTT CCAGTATCCC TTCCACGAGC TGATGGTGTG GGCAGTGCTG
    ATGAAACGCC AGAAAATGGC AGTGTTCCTC TGGCAGCGAG GGGAAGAGAG CATGGCCAAG
    GCCCTGGTGG CCTGCAAGCT CTACAAGGCC ATGGCCCACG AGTCCTCCGA GAGTGATCTG
    GTGGATGACA TCTCCCAGGA CTTGGATAAC AATTCCAAAG ACTTCGGCCA GCTTGCTTTG
    GAGTTATTAG ACCAGTCCTA TAAGCATGAC GAGCAGATCG CTATGAAACT CCTGACCTAC
    GAGCTGAAAA ACTGGAGCAA CTCGACCTGC CTCAAACTGG CCGTGGCAGC CAAACACCGG
    GACTTCATTG CTCACACCTG CAGCCAGATG CTGCTGACCG ATATGTGGAT GGGAAGACTG
    CGGATGCGGA AGAACCCCGG CCTGAAGGTT ATCATGGGGA TTCTTCTACC CCCCACCATC
    TTGTTTTTGG AATTTCGCAC ATATGATGAT TTCTCGTATC AAACATCCAA GGAAAATGAG
    GATGGCAAAG AAAAAGAAGA GGAAAATACG GATGCAAATG CAGATGCTGG CTCAAGAAAG
    GGGGATGAGG AGAACGAGCA CAAAAAACAG AGAAGTATTC CCATCGGAAC AAAGATCTGT
    GAATTCTATA ACGCGCCCAT TGTCAAGTTC TGGTTTTACA CAATATCATA CTTGGGCTAC
    CTGCTGCTGT TTAACTACGT CATCCTGGTG CGGATGGATG GCTGGCCGTC CCTCCAGGAG
    TGGATCGTCA TCTCCTACAT CGTGAGCCTG GCGTTAGAGA AGATACGAGA GATCCTCATG
    TCAGAACCAG GCAAACTCAG CCAGAAAATC AAAGTTTGGC TTCAGGAGTA CTGGAACATC
    ACAGATCTCG TGGCCATTTC CACATTCATG ATTGGAGCAA TTCTTCGCCT ACAGAACCAG
    CCCTACATGG GCTATGGCCG GGTGATCTAC TGTGTGGATA TCATCTTCTG GTACATCCGT
    GTCCTGGACA TCTTTGGTGT CAACAAGTAT CTGGGGCCAT ACGTGATGAT GATTGGAAAG
    ATGATGATCG ACATGCTGTA CTTTGTGGTC ATCATGCTGG TCGTGCTCAT GAGTTTCGGA
    GTAGCCCGTC AAGCCATTCT GCATCCAGAG GAGAAGCCCT CTTGGAAACT GGCCCGAAAC
    ATCTTCTACA TGCCCTACTG GATGATCTAT GGAGAGGTGT TTGCAGACCA GATAGACCTC
    TACGCCATGG AAATTAATCC TCCTTGTGGT GAGAACCTAT ATGATGAGGA GGGCAAGCGG
    CTTCCTCCCT GTATCCCCGG CGCCTGGCTC ACTCCAGCAC TCATGGCGTG CTATCTACTG
    GTCGCCAACA TCCTGCTGGT GAACCTGCTG ATTGCTGTGT TCAACAATAC CTTCTTTGAA
    GTAAAATCAA TATCCAACCA GGTGTGGAAG TTCCAGCGAT ATCAGCTGAT TATGACATTT
    CATGACAGGC CAGTCCTGCC CCCACCGATG ATCATTTTAA GCCACATCTA CATCATCATT
    ATGCGTCTCA GCGGCCGCTG CAGGAAAAAG AGAGAAGGGG ACCAAGAGGA ACGGGATCGT
    GGATTGAAGC TCTTCCTTAG CGACGAGGAG CTAAAGAGGC TGCATGAGTT CGAGGAGCAG
    TGCGTGCAGG AGCACTTCCG GGAGAAGGAG GATGAGCAGC AGTCGTCCAG CGACGAGCGC
    ATCCGGGTCA CTTCTGAAAG AGTTGAAAAT ATGTCAATGA GGTTGGAAGA AATCAATGAA
    AGAGAAACTT TTATGAAAAC TTCCCTGCAG ACTGTTGACC TTCGACTTGC TCAGCTAGAA
    GAATTATCTA ACAGAATGGT GAATGCTCTT GAAAATCTTG CGGGAATCGA CAGGTCTGAC
    CTGATCCAGG CACGGTCCCG GGCTTCTTCT GAATGTGAGG CAACGTATCT TCTCCGGCAA
    AGCAGCATCA ATAGCGCTGA TGGCTACAGC TTGTATCGAT ATCATTTTAA CGGAGAAGAG
    TTATTATTTG AGGATACATC TCTCTCCACG TCACCAGGGA CAGGAGTCAG GAAAAAAACC
    TGTTCCTTCC GTATAAAGGA AGAGAAGGAC GTGAAAACGC ACCTAGTCCC AGAATGTCAG
    AACAGTCTTC ACCTTTCACT GGGCACAAGC ACATCAGCAA CCCCAGATGG CAGTCACCTT
    GCAGTAGATG ACTTAAAGAA CGCTGAAGAG TCAAAATTAG GTCCAGATAT TGGGATTTCA
    AAGGAAGATG ATGAAAGACA GACAGACTCT AAAAAAGAAG AAACTATTTC CCCAAGTTTA
    AATAAAACAG ATGTGATACA TGGACAGGAC AAATCAGATG TTCAAAACAC TCAGCTAACA
    GTGGAAACGA CAAATATAGA AGGCACTATT TCCTATCCCC TGGAAGAAAC CAAAATTACA
    CGCTATTTCC CCGATGAAAC GATCAATGCT TGTAAAACAA TGAAGTCCAG AAGCTTCGTC
    TATTCCCGGG GAAGAAAGCT GGTCGGTGGG GTTAACCAGG ATGTAGAGTA CAGTTCAATC
    ACGGACCAGC AATTGACGAC GGAATGGCAA TGCCAAGTTC AAAAGATCAC GCGCTCTCAT
    AGCACAGATA TTCCTTACAT TGTGTCGGAA GCTGCAGTGC AAGCTGAGCA TAAAGAGCAG
    TTTGCAGATA TGCAAGATGA ACACCATGTC GCTGAAGCAA TTCCTCGAAT CCCTCGCTTG
    TCCCTAACCA TTACTGACAG AAATGGGATG GAAAACTTAC TGTCTGTGAA GCCAGATCAA
    ACTTTGGGAT TCCCATCTCT CAGGTCAAAA AGTTTACATG GACATCCTAG GAATGTGAAA
    TCCATTCAGG GAAAGTTAGA CAGATCTGGA CATGCCAGTA GTGTAAGCAG CTTAGTAATT
    GTGTCTGGAA TGACAGCAGA AGAAAAAAAG GTTAAGAAAG AGAAAGCTTC CACAGAAACT
    GAATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14795
    Clone ID Related Accession (Same CDS sequence) NM_001252030.2 , NM_001252030.1
    Accession Version NM_001252030.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, AC009562.9 and BU741333.1. On Jun 2, 2019 this sequence version replaced NM_001252030.1. Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070356.1, BU933033.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC
    ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT
    CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT
    CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001238959.1
    CDS133..543
    Translation

    Target ORF information:

    RefSeq Version NM_001252030.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252030.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC
    ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT
    CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT
    CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14795
    Clone ID Related Accession (Same CDS sequence) NM_001252030.2 , NM_001252030.1
    Accession Version NM_001252030.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 411bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, AC009562.9 and BU741333.1. Transcript Variant: This variant (4) differs in the 5' UTR, 3' UTR, and coding region, compared to variant 1. The resulting isoform (4) is shorter at the N-terminus and has a distinct C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070356.1, BC058286.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147596, SAMN03465402 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC
    ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT
    CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT
    CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001238959.1
    CDS134..544
    Translation

    Target ORF information:

    RefSeq Version NM_001252030.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252030.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGGTGAGAA AGGCATTCAG ACATGGTGCC
    ACTAGGATCA CAGCTTTCAT TGGCGGCCAG TCTCCCAGCC CCAAACTGCA GATACCTGGT
    CTTCTTCATG GCTGTGGCTC AATCTTCCTA GATATTTCAT TGAAAAACCA AGAGATATAT
    CTGTGCACAT GGCTTTTAGC CATGAGGCTT GGAAACTGGA CACCACTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14262
    Clone ID Related Accession (Same CDS sequence) NM_001252024.2 , NM_001252024.1
    Accession Version NM_001252024.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, HM135790.1, AC009562.9 and BU743036.1. On Jun 2, 2019 this sequence version replaced NM_001252024.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate exon in the 5' coding region. These differences cause translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM135790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
    CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC
    CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG
    CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT
    CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC
    ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG
    CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA
    GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG
    GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG
    TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA
    GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG
    CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC
    AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG
    ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT
    AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT
    TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA
    GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT
    AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG
    AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA
    ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA
    CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG
    CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG
    CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA
    GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG
    AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG
    CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG
    CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA
    AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG
    TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC
    AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT
    GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT
    GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG
    GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC
    ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG
    AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG
    CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC
    CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC
    AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG
    GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC
    CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG
    GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC
    TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA
    AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC
    TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC
    CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG
    ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC
    TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA
    CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG
    TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG
    ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG
    AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG
    GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG
    ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG
    GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC
    TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC
    TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT
    ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC
    ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA
    CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC
    GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC
    GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA
    ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT
    CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC
    AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT
    CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC
    GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG
    AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA
    GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC
    AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT
    GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC
    CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT
    CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC
    AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA
    AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC
    AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG
    CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT
    AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC
    CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG
    CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG
    AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC
    TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC
    ACAGAAACTG AATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001238953.1
    CDS153..5030
    Translation

    Target ORF information:

    RefSeq Version NM_001252024.2
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252024.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
    CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC
    CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG
    CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT
    CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC
    ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG
    CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA
    GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG
    GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG
    TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA
    GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG
    CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC
    AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG
    ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT
    AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT
    TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA
    GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT
    AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG
    AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA
    ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA
    CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG
    CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG
    CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA
    GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG
    AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG
    CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG
    CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA
    AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG
    TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC
    AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT
    GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT
    GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG
    GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC
    ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG
    AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG
    CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC
    CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC
    AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG
    GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC
    CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG
    GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC
    TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA
    AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC
    TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC
    CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG
    ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC
    TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA
    CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG
    TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG
    ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG
    AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG
    GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG
    ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG
    GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC
    TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC
    TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT
    ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC
    ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA
    CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC
    GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC
    GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA
    ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT
    CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC
    AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT
    CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC
    GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG
    AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA
    GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC
    AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT
    GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC
    CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT
    CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC
    AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA
    AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC
    AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG
    CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT
    AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC
    CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG
    CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG
    AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC
    TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC
    ACAGAAACTG AATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14262
    Clone ID Related Accession (Same CDS sequence) NM_001252024.2 , NM_001252024.1
    Accession Version NM_001252024.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4878bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 1 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058286.1, HM135790.1, AC009562.9 and BU743036.1. Transcript Variant: This variant (3) differs in the 5' UTR and uses an alternate exon in the 5' coding region. These differences cause translation initiation at an alternate start codon, compared to variant 1. The encoded isoform (3) has a distinct N-terminus and is shorter than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM135790.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
    CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC
    CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG
    CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT
    CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC
    ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG
    CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA
    GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG
    GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG
    TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA
    GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG
    CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC
    AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG
    ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT
    AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT
    TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA
    GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT
    AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG
    AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA
    ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA
    CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG
    CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG
    CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA
    GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG
    AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG
    CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG
    CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA
    AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG
    TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC
    AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT
    GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT
    GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG
    GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC
    ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG
    AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG
    CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC
    CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC
    AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG
    GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC
    CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG
    GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC
    TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA
    AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC
    TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC
    CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG
    ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC
    TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA
    CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG
    TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG
    ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG
    AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG
    GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG
    ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG
    GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC
    TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC
    TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT
    ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC
    ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA
    CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC
    GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC
    GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA
    ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT
    CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC
    AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT
    CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC
    GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG
    AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA
    GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC
    AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT
    GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC
    CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT
    CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC
    AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA
    AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC
    AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG
    CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT
    AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC
    CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG
    CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG
    AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC
    TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC
    ACAGAAACTG AATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001238953.1
    CDS154..5031
    Translation

    Target ORF information:

    RefSeq Version NM_001252024.1
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001252024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGGGTCAGA AATCTTGGAT AGAGAAAACC TTTTGCAAAC GGGAATGTAT CTTTGTAATT 
    CCTAGCATGA AAGACTCTAA CAGGTGTTGC TGTGGCCAGT TCACCAACCA GCATATCCCC
    CCTCTGCCAA GTGCAACACC CAGCAAAAAT GAAGAGGAAA GCAAACAGGT GGAGACTCAG
    CCTGAGAAAT GGTCTGTTGC CAAGCACACC CAGAGCTACC CAACAGATTC CTATGGAGTT
    CTTGAATTCC AGGGTGGCGG ATATTCCAAT AAAGCCATGT ATATCCGTGT ATCCTATGAC
    ACCAAGCCAG ACTCACTGCT CCATCTCATG GTGAAAGATT GGCAGCTGGA ACTCCCCAAG
    CTCTTAATAT CTGTGCATGG AGGCCTCCAG AACTTTGAGA TGCAGCCCAA GCTGAAACAA
    GTCTTTGGGA AAGGCCTGAT CAAGGCTGCT ATGACCACCG GGGCCTGGAT CTTCACCGGG
    GGTGTCAGCA CAGGTGTTAT CAGCCACGTA GGGGATGCCT TGAAAGACCA CTCCTCCAAG
    TCCAGAGGCC GGGTTTGTGC TATAGGAATT GCTCCATGGG GCATCGTGGA GAATAAGGAA
    GACCTGGTTG GAAAGGATGT AACAAGAGTG TACCAGACCA TGTCCAACCC TCTAAGTAAG
    CTCTCTGTGC TCAACAACTC CCACACCCAC TTCATCCTGG CTGACAATGG CACCCTGGGC
    AAGTATGGCG CCGAGGTGAA GCTGCGAAGG CTGCTGGAAA AGCACATCTC CCTGCAGAAG
    ATCAACACAA GACTGGGGCA GGGCGTGCCC CTCGTGGGTC TCGTGGTGGA GGGGGGCCCT
    AACGTGGTGT CCATCGTCTT GGAATACCTG CAAGAAGAGC CTCCCATCCC TGTGGTGATT
    TGTGATGGCA GCGGACGTGC CTCGGACATC CTGTCCTTTG CGCACAAGTA CTGTGAAGAA
    GGCGGAATAA TAAATGAGTC CCTCAGGGAG CAGCTTCTAG TTACCATTCA GAAAACATTT
    AATTATAATA AGGCACAATC ACATCAGCTG TTTGCAATTA TAATGGAGTG CATGAAGAAG
    AAAGAACTCG TCACTGTGTT CAGAATGGGT TCTGAGGGCC AGCAGGACAT CGAGATGGCA
    ATTTTAACTG CCCTGCTGAA AGGAACAAAC GTATCTGCTC CAGATCAGCT GAGCTTGGCA
    CTGGCTTGGA ACCGCGTGGA CATAGCACGA AGCCAGATCT TTGTCTTTGG GCCCCACTGG
    CCGCCCCTGG GAAGCCTGGC ACCCCCGACG GACAGCAAAG CCACGGAGAA GGAGAAGAAG
    CCACCCATGG CCACCACCAA GGGAGGAAGA GGAAAAGGGA AAGGCAAGAA GAAAGGGAAA
    GTGAAAGAGG AAGTGGAGGA AGAAACTGAC CCCCGGAAGA TAGAGCTGCT GAACTGGGTG
    AATGCTTTGG AGCAAGCGAT GCTAGATGCT TTAGTCTTAG ATCGTGTCGA CTTTGTGAAG
    CTCCTGATTG AAAACGGAGT GAACATGCAA CACTTTCTGA CCATTCCGAG GCTGGAGGAG
    CTTTATAACA CAAGACTGGG TCCACCAAAC ACACTTCATC TGCTGGTGAG GGATGTGAAA
    AAGAGCAACC TTCCGCCTGA TTACCACATC AGCCTCATAG ACATCGGGCT CGTGCTGGAG
    TACCTCATGG GAGGAGCCTA CCGCTGCAAC TACACTCGGA AAAACTTTCG GACCCTTTAC
    AACAACTTGT TTGGACCAAA GAGGCCTAAA GCTCTTAAAC TTCTGGGAAT GGAAGATGAT
    GAGCCTCCAG CTAAAGGGAA GAAAAAGAAA AAGAAGAAAA AGGAGGAAGA GATCGACATT
    GATGTGGACG ACCCTGCCGT GAGTCGGTTC CAGTATCCCT TCCACGAGCT GATGGTGTGG
    GCAGTGCTGA TGAAACGCCA GAAAATGGCA GTGTTCCTCT GGCAGCGAGG GGAAGAGAGC
    ATGGCCAAGG CCCTGGTGGC CTGCAAGCTC TACAAGGCCA TGGCCCACGA GTCCTCCGAG
    AGTGATCTGG TGGATGACAT CTCCCAGGAC TTGGATAACA ATTCCAAAGA CTTCGGCCAG
    CTTGCTTTGG AGTTATTAGA CCAGTCCTAT AAGCATGACG AGCAGATCGC TATGAAACTC
    CTGACCTACG AGCTGAAAAA CTGGAGCAAC TCGACCTGCC TCAAACTGGC CGTGGCAGCC
    AAACACCGGG ACTTCATTGC TCACACCTGC AGCCAGATGC TGCTGACCGA TATGTGGATG
    GGAAGACTGC GGATGCGGAA GAACCCCGGC CTGAAGGTTA TCATGGGGAT TCTTCTACCC
    CCCACCATCT TGTTTTTGGA ATTTCGCACA TATGATGATT TCTCGTATCA AACATCCAAG
    GAAAATGAGG ATGGCAAAGA AAAAGAAGAG GAAAATACGG ATGCAAATGC AGATGCTGGC
    TCAAGAAAGG GGGATGAGGA GAACGAGCAC AAAAAACAGA GAAGTATTCC CATCGGAACA
    AAGATCTGTG AATTCTATAA CGCGCCCATT GTCAAGTTCT GGTTTTACAC AATATCATAC
    TTGGGCTACC TGCTGCTGTT TAACTACGTC ATCCTGGTGC GGATGGATGG CTGGCCGTCC
    CTCCAGGAGT GGATCGTCAT CTCCTACATC GTGAGCCTGG CGTTAGAGAA GATACGAGAG
    ATCCTCATGT CAGAACCAGG CAAACTCAGC CAGAAAATCA AAGTTTGGCT TCAGGAGTAC
    TGGAACATCA CAGATCTCGT GGCCATTTCC ACATTCATGA TTGGAGCAAT TCTTCGCCTA
    CAGAACCAGC CCTACATGGG CTATGGCCGG GTGATCTACT GTGTGGATAT CATCTTCTGG
    TACATCCGTG TCCTGGACAT CTTTGGTGTC AACAAGTATC TGGGGCCATA CGTGATGATG
    ATTGGAAAGA TGATGATCGA CATGCTGTAC TTTGTGGTCA TCATGCTGGT CGTGCTCATG
    AGTTTCGGAG TAGCCCGTCA AGCCATTCTG CATCCAGAGG AGAAGCCCTC TTGGAAACTG
    GCCCGAAACA TCTTCTACAT GCCCTACTGG ATGATCTATG GAGAGGTGTT TGCAGACCAG
    ATAGACCTCT ACGCCATGGA AATTAATCCT CCTTGTGGTG AGAACCTATA TGATGAGGAG
    GGCAAGCGGC TTCCTCCCTG TATCCCCGGC GCCTGGCTCA CTCCAGCACT CATGGCGTGC
    TATCTACTGG TCGCCAACAT CCTGCTGGTG AACCTGCTGA TTGCTGTGTT CAACAATACC
    TTCTTTGAAG TAAAATCAAT ATCCAACCAG GTGTGGAAGT TCCAGCGATA TCAGCTGATT
    ATGACATTTC ATGACAGGCC AGTCCTGCCC CCACCGATGA TCATTTTAAG CCACATCTAC
    ATCATCATTA TGCGTCTCAG CGGCCGCTGC AGGAAAAAGA GAGAAGGGGA CCAAGAGGAA
    CGGGATCGTG GATTGAAGCT CTTCCTTAGC GACGAGGAGC TAAAGAGGCT GCATGAGTTC
    GAGGAGCAGT GCGTGCAGGA GCACTTCCGG GAGAAGGAGG ATGAGCAGCA GTCGTCCAGC
    GACGAGCGCA TCCGGGTCAC TTCTGAAAGA GTTGAAAATA TGTCAATGAG GTTGGAAGAA
    ATCAATGAAA GAGAAACTTT TATGAAAACT TCCCTGCAGA CTGTTGACCT TCGACTTGCT
    CAGCTAGAAG AATTATCTAA CAGAATGGTG AATGCTCTTG AAAATCTTGC GGGAATCGAC
    AGGTCTGACC TGATCCAGGC ACGGTCCCGG GCTTCTTCTG AATGTGAGGC AACGTATCTT
    CTCCGGCAAA GCAGCATCAA TAGCGCTGAT GGCTACAGCT TGTATCGATA TCATTTTAAC
    GGAGAAGAGT TATTATTTGA GGATACATCT CTCTCCACGT CACCAGGGAC AGGAGTCAGG
    AAAAAAACCT GTTCCTTCCG TATAAAGGAA GAGAAGGACG TGAAAACGCA CCTAGTCCCA
    GAATGTCAGA ACAGTCTTCA CCTTTCACTG GGCACAAGCA CATCAGCAAC CCCAGATGGC
    AGTCACCTTG CAGTAGATGA CTTAAAGAAC GCTGAAGAGT CAAAATTAGG TCCAGATATT
    GGGATTTCAA AGGAAGATGA TGAAAGACAG ACAGACTCTA AAAAAGAAGA AACTATTTCC
    CCAAGTTTAA ATAAAACAGA TGTGATACAT GGACAGGACA AATCAGATGT TCAAAACACT
    CAGCTAACAG TGGAAACGAC AAATATAGAA GGCACTATTT CCTATCCCCT GGAAGAAACC
    AAAATTACAC GCTATTTCCC CGATGAAACG ATCAATGCTT GTAAAACAAT GAAGTCCAGA
    AGCTTCGTCT ATTCCCGGGG AAGAAAGCTG GTCGGTGGGG TTAACCAGGA TGTAGAGTAC
    AGTTCAATCA CGGACCAGCA ATTGACGACG GAATGGCAAT GCCAAGTTCA AAAGATCACG
    CGCTCTCATA GCACAGATAT TCCTTACATT GTGTCGGAAG CTGCAGTGCA AGCTGAGCAT
    AAAGAGCAGT TTGCAGATAT GCAAGATGAA CACCATGTCG CTGAAGCAAT TCCTCGAATC
    CCTCGCTTGT CCCTAACCAT TACTGACAGA AATGGGATGG AAAACTTACT GTCTGTGAAG
    CCAGATCAAA CTTTGGGATT CCCATCTCTC AGGTCAAAAA GTTTACATGG ACATCCTAGG
    AATGTGAAAT CCATTCAGGG AAAGTTAGAC AGATCTGGAC ATGCCAGTAG TGTAAGCAGC
    TTAGTAATTG TGTCTGGAAT GACAGCAGAA GAAAAAAAGG TTAAGAAAGA GAAAGCTTCC
    ACAGAAACTG AATGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20829
    Clone ID Related Accession (Same CDS sequence) NM_002420.6 , NM_002420.5
    Accession Version NM_002420.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-18
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU933033.1, AF071787.1, AC009562.9, HM135791.1 and BU743036.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_002420.5. Transcript Variant: This variant (2) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF071787.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG
    CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA
    ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT
    GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC
    AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA
    GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG
    GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT
    GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT
    GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC
    ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG
    GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT
    GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA
    ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT
    AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA
    CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA
    ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT
    TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC
    CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC
    ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA
    GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT
    TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG
    ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT
    AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC
    AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC
    ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC
    TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT
    CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG
    GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG
    CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC
    AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT
    CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT
    TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC
    TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC
    CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA
    CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC
    ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT
    GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA
    AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC
    TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC
    TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG
    GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC
    ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC
    ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC
    CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC
    CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA
    AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC
    GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA
    AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC
    CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG
    CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA
    CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT
    GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA
    TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC
    ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT
    CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG
    CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG
    CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT
    GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA
    GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT
    GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG
    CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA
    GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA
    ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT
    CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC
    CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT
    TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT
    TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA
    ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT
    ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC
    GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA
    ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT
    CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG
    CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC
    TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT
    CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG
    AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA
    ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA
    ACTGAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002411.3
    CDS133..4944
    Translation

    Target ORF information:

    RefSeq Version NM_002420.6
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002420.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG
    CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA
    ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT
    GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC
    AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA
    GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG
    GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT
    GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT
    GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC
    ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG
    GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT
    GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA
    ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT
    AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA
    CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA
    ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT
    TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC
    CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC
    ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA
    GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT
    TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG
    ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT
    AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC
    AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC
    ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC
    TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT
    CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG
    GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG
    CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC
    AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT
    CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT
    TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC
    TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC
    CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA
    CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC
    ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT
    GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA
    AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC
    TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC
    TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG
    GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC
    ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC
    ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC
    CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC
    CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA
    AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC
    GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA
    AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC
    CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG
    CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA
    CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT
    GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA
    TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC
    ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT
    CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG
    CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG
    CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT
    GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA
    GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT
    GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG
    CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA
    GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA
    ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT
    CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC
    CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT
    TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT
    TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA
    ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT
    ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC
    GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA
    ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT
    CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG
    CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC
    TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT
    CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG
    AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA
    ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA
    ACTGAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu20829
    Clone ID Related Accession (Same CDS sequence) NM_002420.6 , NM_002420.5
    Accession Version NM_002420.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4812bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Homo sapiens(human)
    Product transient receptor potential cation channel subfamily M member 1 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU933033.1, AF071787.1, AC009562.9, HM135791.1 and BU743036.1. This sequence is a reference standard in the RefSeqGene project. On or before Mar 22, 2015 this sequence version replaced XM_011548920.1, XM_011547404.1, XM_006725544.1, NM_002420.4. Transcript Variant: This variant (2) differs in the 5' UTR and lacks a portion of the 5' coding region, compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (2) with a shorter N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF071787.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968832 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG
    CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA
    ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT
    GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC
    AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA
    GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG
    GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT
    GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT
    GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC
    ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG
    GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT
    GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA
    ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT
    AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA
    CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA
    ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT
    TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC
    CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC
    ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA
    GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT
    TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG
    ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT
    AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC
    AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC
    ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC
    TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT
    CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG
    GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG
    CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC
    AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT
    CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT
    TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC
    TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC
    CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA
    CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC
    ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT
    GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA
    AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC
    TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC
    TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG
    GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC
    ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC
    ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC
    CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC
    CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA
    AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC
    GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA
    AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC
    CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG
    CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA
    CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT
    GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA
    TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC
    ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT
    CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG
    CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG
    CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT
    GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA
    GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT
    GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG
    CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA
    GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA
    ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT
    CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC
    CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT
    TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT
    TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA
    ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT
    ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC
    GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA
    ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT
    CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG
    CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC
    TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT
    CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG
    AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA
    ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA
    ACTGAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002411.3
    CDS134..4945
    Translation

    Target ORF information:

    RefSeq Version NM_002420.5
    Organism Homo sapiens(human)
    Definition Homo sapiens transient receptor potential cation channel subfamily M member 1 (TRPM1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002420.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAAAGACT CTAACAGGTG TTGCTGTGGC CAGTTCACCA ACCAGCATAT CCCCCCTCTG 
    CCAAGTGCAA CACCCAGCAA AAATGAAGAG GAAAGCAAAC AGGTGGAGAC TCAGCCTGAG
    AAATGGTCTG TTGCCAAGCA CACCCAGAGC TACCCAACAG ATTCCTATGG AGTTCTTGAA
    TTCCAGGGTG GCGGATATTC CAATAAAGCC ATGTATATCC GTGTATCCTA TGACACCAAG
    CCAGACTCAC TGCTCCATCT CATGGTGAAA GATTGGCAGC TGGAACTCCC CAAGCTCTTA
    ATATCTGTGC ATGGAGGCCT CCAGAACTTT GAGATGCAGC CCAAGCTGAA ACAAGTCTTT
    GGGAAAGGCC TGATCAAGGC TGCTATGACC ACCGGGGCCT GGATCTTCAC CGGGGGTGTC
    AGCACAGGTG TTATCAGCCA CGTAGGGGAT GCCTTGAAAG ACCACTCCTC CAAGTCCAGA
    GGCCGGGTTT GTGCTATAGG AATTGCTCCA TGGGGCATCG TGGAGAATAA GGAAGACCTG
    GTTGGAAAGG ATGTAACAAG AGTGTACCAG ACCATGTCCA ACCCTCTAAG TAAGCTCTCT
    GTGCTCAACA ACTCCCACAC CCACTTCATC CTGGCTGACA ATGGCACCCT GGGCAAGTAT
    GGCGCCGAGG TGAAGCTGCG AAGGCTGCTG GAAAAGCACA TCTCCCTGCA GAAGATCAAC
    ACAAGACTGG GGCAGGGCGT GCCCCTCGTG GGTCTCGTGG TGGAGGGGGG CCCTAACGTG
    GTGTCCATCG TCTTGGAATA CCTGCAAGAA GAGCCTCCCA TCCCTGTGGT GATTTGTGAT
    GGCAGCGGAC GTGCCTCGGA CATCCTGTCC TTTGCGCACA AGTACTGTGA AGAAGGCGGA
    ATAATAAATG AGTCCCTCAG GGAGCAGCTT CTAGTTACCA TTCAGAAAAC ATTTAATTAT
    AATAAGGCAC AATCACATCA GCTGTTTGCA ATTATAATGG AGTGCATGAA GAAGAAAGAA
    CTCGTCACTG TGTTCAGAAT GGGTTCTGAG GGCCAGCAGG ACATCGAGAT GGCAATTTTA
    ACTGCCCTGC TGAAAGGAAC AAACGTATCT GCTCCAGATC AGCTGAGCTT GGCACTGGCT
    TGGAACCGCG TGGACATAGC ACGAAGCCAG ATCTTTGTCT TTGGGCCCCA CTGGCCGCCC
    CTGGGAAGCC TGGCACCCCC GACGGACAGC AAAGCCACGG AGAAGGAGAA GAAGCCACCC
    ATGGCCACCA CCAAGGGAGG AAGAGGAAAA GGGAAAGGCA AGAAGAAAGG GAAAGTGAAA
    GAGGAAGTGG AGGAAGAAAC TGACCCCCGG AAGATAGAGC TGCTGAACTG GGTGAATGCT
    TTGGAGCAAG CGATGCTAGA TGCTTTAGTC TTAGATCGTG TCGACTTTGT GAAGCTCCTG
    ATTGAAAACG GAGTGAACAT GCAACACTTT CTGACCATTC CGAGGCTGGA GGAGCTTTAT
    AACACAAGAC TGGGTCCACC AAACACACTT CATCTGCTGG TGAGGGATGT GAAAAAGAGC
    AACCTTCCGC CTGATTACCA CATCAGCCTC ATAGACATCG GGCTCGTGCT GGAGTACCTC
    ATGGGAGGAG CCTACCGCTG CAACTACACT CGGAAAAACT TTCGGACCCT TTACAACAAC
    TTGTTTGGAC CAAAGAGGCC TAAAGCTCTT AAACTTCTGG GAATGGAAGA TGATGAGCCT
    CCAGCTAAAG GGAAGAAAAA GAAAAAGAAG AAAAAGGAGG AAGAGATCGA CATTGATGTG
    GACGACCCTG CCGTGAGTCG GTTCCAGTAT CCCTTCCACG AGCTGATGGT GTGGGCAGTG
    CTGATGAAAC GCCAGAAAAT GGCAGTGTTC CTCTGGCAGC GAGGGGAAGA GAGCATGGCC
    AAGGCCCTGG TGGCCTGCAA GCTCTACAAG GCCATGGCCC ACGAGTCCTC CGAGAGTGAT
    CTGGTGGATG ACATCTCCCA GGACTTGGAT AACAATTCCA AAGACTTCGG CCAGCTTGCT
    TTGGAGTTAT TAGACCAGTC CTATAAGCAT GACGAGCAGA TCGCTATGAA ACTCCTGACC
    TACGAGCTGA AAAACTGGAG CAACTCGACC TGCCTCAAAC TGGCCGTGGC AGCCAAACAC
    CGGGACTTCA TTGCTCACAC CTGCAGCCAG ATGCTGCTGA CCGATATGTG GATGGGAAGA
    CTGCGGATGC GGAAGAACCC CGGCCTGAAG GTTATCATGG GGATTCTTCT ACCCCCCACC
    ATCTTGTTTT TGGAATTTCG CACATATGAT GATTTCTCGT ATCAAACATC CAAGGAAAAT
    GAGGATGGCA AAGAAAAAGA AGAGGAAAAT ACGGATGCAA ATGCAGATGC TGGCTCAAGA
    AAGGGGGATG AGGAGAACGA GCACAAAAAA CAGAGAAGTA TTCCCATCGG AACAAAGATC
    TGTGAATTCT ATAACGCGCC CATTGTCAAG TTCTGGTTTT ACACAATATC ATACTTGGGC
    TACCTGCTGC TGTTTAACTA CGTCATCCTG GTGCGGATGG ATGGCTGGCC GTCCCTCCAG
    GAGTGGATCG TCATCTCCTA CATCGTGAGC CTGGCGTTAG AGAAGATACG AGAGATCCTC
    ATGTCAGAAC CAGGCAAACT CAGCCAGAAA ATCAAAGTTT GGCTTCAGGA GTACTGGAAC
    ATCACAGATC TCGTGGCCAT TTCCACATTC ATGATTGGAG CAATTCTTCG CCTACAGAAC
    CAGCCCTACA TGGGCTATGG CCGGGTGATC TACTGTGTGG ATATCATCTT CTGGTACATC
    CGTGTCCTGG ACATCTTTGG TGTCAACAAG TATCTGGGGC CATACGTGAT GATGATTGGA
    AAGATGATGA TCGACATGCT GTACTTTGTG GTCATCATGC TGGTCGTGCT CATGAGTTTC
    GGAGTAGCCC GTCAAGCCAT TCTGCATCCA GAGGAGAAGC CCTCTTGGAA ACTGGCCCGA
    AACATCTTCT ACATGCCCTA CTGGATGATC TATGGAGAGG TGTTTGCAGA CCAGATAGAC
    CTCTACGCCA TGGAAATTAA TCCTCCTTGT GGTGAGAACC TATATGATGA GGAGGGCAAG
    CGGCTTCCTC CCTGTATCCC CGGCGCCTGG CTCACTCCAG CACTCATGGC GTGCTATCTA
    CTGGTCGCCA ACATCCTGCT GGTGAACCTG CTGATTGCTG TGTTCAACAA TACCTTCTTT
    GAAGTAAAAT CAATATCCAA CCAGGTGTGG AAGTTCCAGC GATATCAGCT GATTATGACA
    TTTCATGACA GGCCAGTCCT GCCCCCACCG ATGATCATTT TAAGCCACAT CTACATCATC
    ATTATGCGTC TCAGCGGCCG CTGCAGGAAA AAGAGAGAAG GGGACCAAGA GGAACGGGAT
    CGTGGATTGA AGCTCTTCCT TAGCGACGAG GAGCTAAAGA GGCTGCATGA GTTCGAGGAG
    CAGTGCGTGC AGGAGCACTT CCGGGAGAAG GAGGATGAGC AGCAGTCGTC CAGCGACGAG
    CGCATCCGGG TCACTTCTGA AAGAGTTGAA AATATGTCAA TGAGGTTGGA AGAAATCAAT
    GAAAGAGAAA CTTTTATGAA AACTTCCCTG CAGACTGTTG ACCTTCGACT TGCTCAGCTA
    GAAGAATTAT CTAACAGAAT GGTGAATGCT CTTGAAAATC TTGCGGGAAT CGACAGGTCT
    GACCTGATCC AGGCACGGTC CCGGGCTTCT TCTGAATGTG AGGCAACGTA TCTTCTCCGG
    CAAAGCAGCA TCAATAGCGC TGATGGCTAC AGCTTGTATC GATATCATTT TAACGGAGAA
    GAGTTATTAT TTGAGGATAC ATCTCTCTCC ACGTCACCAG GGACAGGAGT CAGGAAAAAA
    ACCTGTTCCT TCCGTATAAA GGAAGAGAAG GACGTGAAAA CGCACCTAGT CCCAGAATGT
    CAGAACAGTC TTCACCTTTC ACTGGGCACA AGCACATCAG CAACCCCAGA TGGCAGTCAC
    CTTGCAGTAG ATGACTTAAA GAACGCTGAA GAGTCAAAAT TAGGTCCAGA TATTGGGATT
    TCAAAGGAAG ATGATGAAAG ACAGACAGAC TCTAAAAAAG AAGAAACTAT TTCCCCAAGT
    TTAAATAAAA CAGATGTGAT ACATGGACAG GACAAATCAG ATGTTCAAAA CACTCAGCTA
    ACAGTGGAAA CGACAAATAT AGAAGGCACT ATTTCCTATC CCCTGGAAGA AACCAAAATT
    ACACGCTATT TCCCCGATGA AACGATCAAT GCTTGTAAAA CAATGAAGTC CAGAAGCTTC
    GTCTATTCCC GGGGAAGAAA GCTGGTCGGT GGGGTTAACC AGGATGTAGA GTACAGTTCA
    ATCACGGACC AGCAATTGAC GACGGAATGG CAATGCCAAG TTCAAAAGAT CACGCGCTCT
    CATAGCACAG ATATTCCTTA CATTGTGTCG GAAGCTGCAG TGCAAGCTGA GCATAAAGAG
    CAGTTTGCAG ATATGCAAGA TGAACACCAT GTCGCTGAAG CAATTCCTCG AATCCCTCGC
    TTGTCCCTAA CCATTACTGA CAGAAATGGG ATGGAAAACT TACTGTCTGT GAAGCCAGAT
    CAAACTTTGG GATTCCCATC TCTCAGGTCA AAAAGTTTAC ATGGACATCC TAGGAATGTG
    AAATCCATTC AGGGAAAGTT AGACAGATCT GGACATGCCA GTAGTGTAAG CAGCTTAGTA
    ATTGTGTCTG GAATGACAGC AGAAGAAAAA AAGGTTAAGA AAGAGAAAGC TTCCACAGAA
    ACTGAATGCT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.