MLF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MLF1
Entrez Gene ID	4291
Full Name	myeloid leukemia factor 1
General protein information	Preferred Names myeloid leukemia factor 1 Names myeloid leukemia factor 1 myelodysplasia-myeloid leukemia factor 1 testis tissue sperm-binding protein Li 49e
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q25.32
Summary	This gene encodes an oncoprotein which is thought to play a role in the phenotypic determination of hemopoetic cells. Translocations between this gene and nucleophosmin have been associated with myelodysplastic syndrome and acute myeloid leukemia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2010].
Disorder MIM:	601402
Disorder Html:	Leukemia, acute myeloid, 601626 (1)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011512853.1	XP_011511155.1	myeloid leukemia factor 1 isoform X1
NM_001195433.1	NP_001182362.1	myeloid leukemia factor 1 isoform 4
NM_022443.4	NP_071888.1	myeloid leukemia factor 1 isoform 1
XM_005247479.5	XP_005247536.1	myeloid leukemia factor 1 isoform X1
XM_005247479.4	XP_005247536.1	myeloid leukemia factor 1 isoform X1
XM_011512853.2	XP_011511155.1	myeloid leukemia factor 1 isoform X3
NM_001130157.3	NP_001123629.1	myeloid leukemia factor 1 isoform 2
NM_001369783.1	NP_001356712.1	myeloid leukemia factor 1 isoform 6
NM_001195433.2	NP_001182362.1	myeloid leukemia factor 1 isoform 4
XM_011512854.2	XP_011511156.1	myeloid leukemia factor 1 isoform X5
NM_001130156.2	NP_001123628.1	myeloid leukemia factor 1 isoform 2
NM_001195432.1	NP_001182361.1	myeloid leukemia factor 1 isoform 3
NM_001195432.2	NP_001182361.1	myeloid leukemia factor 1 isoform 3
NM_001130157.2	NP_001123629.1	myeloid leukemia factor 1 isoform 2
NM_001195434.1	NP_001182363.1	myeloid leukemia factor 1 isoform 5
NM_022443.5	NP_071888.1	myeloid leukemia factor 1 isoform 1
NM_001130156.3	NP_001123628.1	myeloid leukemia factor 1 isoform 2
NM_001195434.2	NP_001182363.1	myeloid leukemia factor 1 isoform 5
NM_001369785.1	NP_001356714.1	myeloid leukemia factor 1 isoform 5
NM_001369781.1	NP_001356710.1	myeloid leukemia factor 1 isoform 7
XM_005247480.2	XP_005247537.1	myeloid leukemia factor 1 isoform X2
XM_006713645.2	XP_006713708.1	myeloid leukemia factor 1 isoform X4
XM_011512852.2	XP_011511154.1	myeloid leukemia factor 1 isoform X2
XM_011512852.3	XP_011511154.1	myeloid leukemia factor 1 isoform X2
NM_001369782.1	NP_001356711.1	myeloid leukemia factor 1 isoform 8
NM_001369784.1	NP_001356713.1	myeloid leukemia factor 1 isoform 2

Pathways from BioSystems

Homologies

	MLF1	XP_001102054.1
Gallus gallus (chicken)	MLF1	XP_004943599.1
Pan troglodytes (chimpanzee)	MLF1	XP_516842.2
Xenopus tropicalis (tropical clawed frog)	mlf1	XP_002933287.1
Mus musculus (house mouse)	Mlf1	NP_001034632.1
Danio rerio (zebrafish)	mlf1	XP_003200041.2
Homo sapiens (human)	MLF1	NP_001182361.1
Canis lupus familiaris (dog)	MLF1	XP_005634670.1
Bos taurus (cattle)	MLF1	NP_001033247.1
Rattus norvegicus (Norway rat)	Mlf1	NP_001101150.1

Gene Ontology
Bibliography

Related articles in PubMed

Myeloid Leukemia Factor Acts in a Chaperone Complex to Regulate Transcription Factor Stability and Gene Expression.
Dyer JO, Dutta A, Gogol M, Weake VM, Dialynas G, Wu X, Seidel C, Zhang Y, Florens L, Washburn MP, Abmayr SM, Workman JL
Journal of molecular biology429(13)2093-2107(2017 Jun)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Common variants upstream of MLF1 at 3q25 and within CPZ at 4p16 associated with neuroblastoma.
McDaniel LD, Conkrite KL, Chang X, Capasso M, Vaksman Z, Oldridge DA, Zachariou A, Horn M, Diamond M, Hou C, Iolascon A, Hakonarson H, Rahman N, Devoto M, Diskin SJ
PLoS genetics13(5)e1006787(2017 May)

MLF1 is a proapoptotic antagonist of HOP complex-mediated survival.
Sun Y, Chao JR, Xu W, Pourpak A, Boyd K, Moshiach S, Qi GY, Fu A, Shao HR, Pounds S, Morris SW
Biochimica et biophysica acta1864(4)719-727(2017 04)

Modulation of mutant Huntingtin aggregates and toxicity by human myeloid leukemia factors.
Banerjee M, Datta M, Bhattacharyya NP
The international journal of biochemistry & cell biology821-9(2017 01)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings suggest that MLF and the associated co-chaperones play a direct role in modulating gene transcription.
Title: Myeloid Leukemia Factor Acts in a Chaperone Complex to Regulate Transcription Factor Stability and Gene Expression.

SNP associated with neuroblastoma resides upstream of the MLF1. Gene silencing of MLF1 in neuroblastoma cells results in significant growth inhibition.
Title: Common variants upstream of MLF1 at 3q25 and within CPZ at 4p16 associated with neuroblastoma.

The data indicate that MLF1 serves as a proapoptotic antagonist that interacts with the HAX1/HtrA2-OMI/PARL (HOP) mitochondrial complex to modulate cell survival.
Title: MLF1 is a proapoptotic antagonist of HOP complex-mediated survival.

Mutation in HTT causes Huntington's disease (HD); aggregates of mutated HTT cause apoptosis in neurons of HD patients. Data suggest that both MLF1 and MLF2 preferentially interact with mutated N-terminal HTT; MLF1/MLF2 reduce number of neurons (Neuro2A cell line) containing mutant HTT aggregates and subsequent apoptosis. (HTT = Huntingtin protein; MLF = myeloid leukemia factor)
Title: Modulation of mutant Huntingtin aggregates and toxicity by human myeloid leukemia factors.

Data indicte that acute myeloid leukemia (AML) with NPM1-MLF1 and AML with NPM1 mutations showed similar immunophenotypical and molecular features, including gene mutation patterns and gene expression profiling (GEP).
Title: Acute myeloid leukemia with translocation t(3;5): new molecular insights.

The following MLF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MLF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07024	NM_001130156.3 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu07024	NM_001130157.3 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu07024	NM_001369784.1 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu55727	NM_022443.5 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu07024	XM_011512853.2 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu58983	NM_001369782.1 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu58983	XM_011512854.2 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
OHu20508	NM_001195432.2 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu32246	NM_001195434.2 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu32246	NM_001369785.1 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu32345	NM_001195433.2 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu37959	NM_001369783.1 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu37960	NM_001369781.1 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu32246	XM_011512852.3 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu32246	XM_005247480.2 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu37959	XM_005247479.5 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu37960	XM_006713645.2 Latest version!	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu37959	XM_005247479.4	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu07024	XM_011512853.1	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu32345	NM_001195433.1	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu20508	NM_001195432.1	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
OHu07024	NM_001130157.2	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu55727	NM_022443.4	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
OHu07024	NM_001130156.2	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu32246	NM_001195434.1	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu32246	XM_011512852.2	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MLF1 ( NM_001130157.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123629.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07024
Clone ID Related Accession (Same CDS sequence)	XM_011512853.1 , XM_011512853.2 , NM_001130157.3 , NM_001130156.2 , NM_001130157.2 , NM_001130156.3 , NM_001369784.1
Accession Version	NM_001130157.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AY848701.1, AC025033.20 and BX647666.1. On Jun 2, 2019 this sequence version replaced NM_001130157.2. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 2, which is shorter than isoform 3. Variants 2, 3 and 10 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.109585.1, SRR1803614.396987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123629.1
CDS	300..1031
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001130157.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130157.3

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001130156.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123628.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07024
Clone ID Related Accession (Same CDS sequence)	XM_011512853.1 , XM_011512853.2 , NM_001130157.3 , NM_001130156.2 , NM_001130157.2 , NM_001130156.3 , NM_001369784.1
Accession Version	NM_001130156.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AY848702.1, BX647666.1 and BU619178.1. On Sep 9, 2010 this sequence version replaced NM_001130156.1. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 2, which is shorter than isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005672.1, AY848702.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123628.1
CDS	257..988
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001130156.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130156.2

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001130157.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123629.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07024
Clone ID Related Accession (Same CDS sequence)	XM_011512853.1 , XM_011512853.2 , NM_001130157.3 , NM_001130156.2 , NM_001130157.2 , NM_001130156.3 , NM_001369784.1
Accession Version	NM_001130157.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AY848701.1, AC025033.20, BX647666.1 and BU619178.1. On Sep 9, 2010 this sequence version replaced NM_001130157.1. Transcript Variant: This variant (2) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 2, which is shorter than isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.109585.1, SRR1660807.93851.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123629.1
CDS	330..1061
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001130157.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130157.2

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001130156.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001123628.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu07024
Clone ID Related Accession (Same CDS sequence)	XM_011512853.1 , XM_011512853.2 , NM_001130157.3 , NM_001130156.2 , NM_001130157.2 , NM_001130156.3 , NM_001369784.1
Accession Version	NM_001130156.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AY848702.1 and BX647666.1. On Jun 2, 2019 this sequence version replaced NM_001130156.2. Transcript Variant: This variant (3) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 2, which is shorter than isoform 3. Variants 2, 3 and 10 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY848702.1, SRR1803616.189573.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001123628.1
CDS	227..958
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001130156.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001130156.3

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001369784.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356713.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 10, mRNA.

Price & Availability↑

CloneID	OHu07024
Clone ID Related Accession (Same CDS sequence)	XM_011512853.1 , XM_011512853.2 , NM_001130157.3 , NM_001130156.2 , NM_001130157.2 , NM_001130156.3 , NM_001369784.1
Accession Version	NM_001369784.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106707.9 and AC025033.20. On Apr 18, 2019 this sequence version replaced XM_011512853.2. Transcript Variant: Variants 2, 3 and 10 encode the same isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.271460.1, SRR1803614.308958.1 [ECO:0000332] ##Evidence-Data-END##

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356713.1
CDS	256..987
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001369784.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369784.1

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_022443.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071888.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55727
Clone ID Related Accession (Same CDS sequence)	NM_022443.4 , NM_022443.5
Accession Version	NM_022443.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AK315402.1, BC007045.1, BX647666.1 and BU619178.1. On Sep 9, 2010 this sequence version replaced NM_022443.3. Transcript Variant: This variant (1) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 1, which is shorter than isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.223641.1, SRR1803615.153486.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTCATAC AGATGTCAGC TCTTTCCAGA CAATGGACCA AATGGTGTCA 
AATATGAGAA ACTATATGCA GAAATTAGAA AGAAACTTCG GTCAACTTTC AGTGGATCCA 
AATGGACATT CATTTTGTTC TTCCTCAGTT ATGACTTATT CCAAAATAGG AGATGAACCG 
CCAAAGGTTT TTCAGGCCTC AACTCAAACT CGTCGAGCTC CAGGAGGAAT AAAGGAAACC 
AGGAAAGCAA TGAGAGATTC TGACAGTGGA CTAGAAAAAA TGGCTATTGG TCATCATATC 
CATGACCGAG CTCATGTCAT TAAAAAGTCA AAGAACAAGA AGACTGGAGA TGAAGAGGTC 
AACCAGGAGT TCATCAATAT GAATGAAAGT GATGCTCATG CTTTTGATGA GGAGTGGCAA 
AGTGAGGTTT TGAAGTACAA ACCAGGACGA CACAATCTAG GAAACACTAG AATGAGAAGT 
GTTGGCCATG AGAATCCTGG CTCCCGAGAA CTTAAAAGAA GGGAGAAACC TCAACAAAGT 
CCAGCCATTG AACATGGAAG GAGATCAAAT GTTTTGGGGG ACAAACTCCA CATCAAAGGC 
TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071888.1
CDS	138..944
Translation	MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_022443.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022443.4

ORF Insert Sequence:

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTCATAC AGATGTCAGC TCTTTCCAGA CAATGGACCA AATGGTGTCA 
AATATGAGAA ACTATATGCA GAAATTAGAA AGAAACTTCG GTCAACTTTC AGTGGATCCA 
AATGGACATT CATTTTGTTC TTCCTCAGTT ATGACTTATT CCAAAATAGG AGATGAACCG 
CCAAAGGTTT TTCAGGCCTC AACTCAAACT CGTCGAGCTC CAGGAGGAAT AAAGGAAACC 
AGGAAAGCAA TGAGAGATTC TGACAGTGGA CTAGAAAAAA TGGCTATTGG TCATCATATC 
CATGACCGAG CTCATGTCAT TAAAAAGTCA AAGAACAAGA AGACTGGAGA TGAAGAGGTC 
AACCAGGAGT TCATCAATAT GAATGAAAGT GATGCTCATG CTTTTGATGA GGAGTGGCAA 
AGTGAGGTTT TGAAGTACAA ACCAGGACGA CACAATCTAG GAAACACTAG AATGAGAAGT 
GTTGGCCATG AGAATCCTGG CTCCCGAGAA CTTAAAAGAA GGGAGAAACC TCAACAAAGT 
CCAGCCATTG AACATGGAAG GAGATCAAAT GTTTTGGGGG ACAAACTCCA CATCAAAGGC 
TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_022443.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071888.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu55727
Clone ID Related Accession (Same CDS sequence)	NM_022443.4 , NM_022443.5
Accession Version	NM_022443.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 807bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315402.1, KU178186.1, AC025033.20 and AA707847.1. This sequence is a reference standard in the RefSeqGene project. On Apr 18, 2019 this sequence version replaced NM_022443.4. Transcript Variant: This variant (1) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 1, which is shorter than isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.223641.1, SRR1803617.54855.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTCATAC AGATGTCAGC TCTTTCCAGA CAATGGACCA AATGGTGTCA 
AATATGAGAA ACTATATGCA GAAATTAGAA AGAAACTTCG GTCAACTTTC AGTGGATCCA 
AATGGACATT CATTTTGTTC TTCCTCAGTT ATGACTTATT CCAAAATAGG AGATGAACCG 
CCAAAGGTTT TTCAGGCCTC AACTCAAACT CGTCGAGCTC CAGGAGGAAT AAAGGAAACC 
AGGAAAGCAA TGAGAGATTC TGACAGTGGA CTAGAAAAAA TGGCTATTGG TCATCATATC 
CATGACCGAG CTCATGTCAT TAAAAAGTCA AAGAACAAGA AGACTGGAGA TGAAGAGGTC 
AACCAGGAGT TCATCAATAT GAATGAAAGT GATGCTCATG CTTTTGATGA GGAGTGGCAA 
AGTGAGGTTT TGAAGTACAA ACCAGGACGA CACAATCTAG GAAACACTAG AATGAGAAGT 
GTTGGCCATG AGAATCCTGG CTCCCGAGAA CTTAAAAGAA GGGAGAAACC TCAACAAAGT 
CCAGCCATTG AACATGGAAG GAGATCAAAT GTTTTGGGGG ACAAACTCCA CATCAAAGGC 
TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071888.1
CDS	108..914
Translation	MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_022443.5
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022443.5

ORF Insert Sequence:

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTCATAC AGATGTCAGC TCTTTCCAGA CAATGGACCA AATGGTGTCA 
AATATGAGAA ACTATATGCA GAAATTAGAA AGAAACTTCG GTCAACTTTC AGTGGATCCA 
AATGGACATT CATTTTGTTC TTCCTCAGTT ATGACTTATT CCAAAATAGG AGATGAACCG 
CCAAAGGTTT TTCAGGCCTC AACTCAAACT CGTCGAGCTC CAGGAGGAAT AAAGGAAACC 
AGGAAAGCAA TGAGAGATTC TGACAGTGGA CTAGAAAAAA TGGCTATTGG TCATCATATC 
CATGACCGAG CTCATGTCAT TAAAAAGTCA AAGAACAAGA AGACTGGAGA TGAAGAGGTC 
AACCAGGAGT TCATCAATAT GAATGAAAGT GATGCTCATG CTTTTGATGA GGAGTGGCAA 
AGTGAGGTTT TGAAGTACAA ACCAGGACGA CACAATCTAG GAAACACTAG AATGAGAAGT 
GTTGGCCATG AGAATCCTGG CTCCCGAGAA CTTAAAAGAA GGGAGAAACC TCAACAAAGT 
CCAGCCATTG AACATGGAAG GAGATCAAAT GTTTTGGGGG ACAAACTCCA CATCAAAGGC 
TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_011512853.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511155.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu07024
Clone ID Related Accession (Same CDS sequence)	XM_011512853.1 , XM_011512853.2 , NM_001130157.3 , NM_001130156.2 , NM_001130157.2 , NM_001130156.3 , NM_001369784.1
Accession Version	XM_011512853.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	myeloid leukemia factor 1 isoform X1
Comment

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511155.1
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_011512853.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512853.1

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_011512853.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511155.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu07024
Clone ID Related Accession (Same CDS sequence)	XM_011512853.1 , XM_011512853.2 , NM_001130157.3 , NM_001130156.2 , NM_001130157.2 , NM_001130156.3 , NM_001369784.1
Accession Version	XM_011512853.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512853.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511155.1
CDS	280..1011
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_011512853.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512853.2

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001369782.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356711.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu58983
Clone ID Related Accession (Same CDS sequence)	XM_011512854.2 , NM_001369782.1
Accession Version	NM_001369782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 8
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106707.9, KU178188.1 and AC025033.20. On Apr 18, 2019 this sequence version replaced XM_011512854.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.188011.1, SRR1803615.205518.1 [ECO:0000332] ##Evidence-Data-END##

ATGGACCAAA TGGTGTCAAA TATGAGAAAC TATATGCAGA AATTAGAAAG AAACTTCGGT 
CAACTTTCAG TGGATCCAAA TGGACATTCA TTTTGTTCTT CCTCAGTTAT GACTTATTCC 
AAAATAGGAG ATGAACCGCC AAAGGTTTTT CAGGCCTCAA CTCAAACTCG TCGAGCTCCA 
GGAGGAATAA AGGAAACCAG GAAAGCAATG AGAGATTCTG ACAGTGGACT AGAAAAAATG 
GCTATTGGTC ATCATATCCA TGACCGAGCT CATGTCATTA AAAAGTCAAA GAACAAGAAG 
ACTGGAGATG AAGAGGTCAA CCAGGAGTTC ATCAATATGA ATGAAAGTGA TGCTCATGCT 
TTTGATGAGG AGTGGCAAAG TGAGGTTTTG AAGTACAAAC CAGGACGACA CAATCTAGGA 
AACACTAGAA TGAGAAGTGT TGGCCATGAG AATCCTGGCT CCCGAGAACT TAAAAGAAGG 
GAGAAACCTC AACAAAGTCC AGCCATTGAA CATGGAAGGA GATCAAATGT TTTGGGGGAC 
AAACTCCACA TCAAAGGCTC ATCTGTGAAA AGCAACAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356711.1
CDS	182..766
Translation	MDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001369782.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369782.1

ORF Insert Sequence:

ATGGACCAAA TGGTGTCAAA TATGAGAAAC TATATGCAGA AATTAGAAAG AAACTTCGGT 
CAACTTTCAG TGGATCCAAA TGGACATTCA TTTTGTTCTT CCTCAGTTAT GACTTATTCC 
AAAATAGGAG ATGAACCGCC AAAGGTTTTT CAGGCCTCAA CTCAAACTCG TCGAGCTCCA 
GGAGGAATAA AGGAAACCAG GAAAGCAATG AGAGATTCTG ACAGTGGACT AGAAAAAATG 
GCTATTGGTC ATCATATCCA TGACCGAGCT CATGTCATTA AAAAGTCAAA GAACAAGAAG 
ACTGGAGATG AAGAGGTCAA CCAGGAGTTC ATCAATATGA ATGAAAGTGA TGCTCATGCT 
TTTGATGAGG AGTGGCAAAG TGAGGTTTTG AAGTACAAAC CAGGACGACA CAATCTAGGA 
AACACTAGAA TGAGAAGTGT TGGCCATGAG AATCCTGGCT CCCGAGAACT TAAAAGAAGG 
GAGAAACCTC AACAAAGTCC AGCCATTGAA CATGGAAGGA GATCAAATGT TTTGGGGGAC 
AAACTCCACA TCAAAGGCTC ATCTGTGAAA AGCAACAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_011512854.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511156.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu58983
Clone ID Related Accession (Same CDS sequence)	XM_011512854.2 , NM_001369782.1
Accession Version	XM_011512854.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 585bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACCAAA TGGTGTCAAA TATGAGAAAC TATATGCAGA AATTAGAAAG AAACTTCGGT 
CAACTTTCAG TGGATCCAAA TGGACATTCA TTTTGTTCTT CCTCAGTTAT GACTTATTCC 
AAAATAGGAG ATGAACCGCC AAAGGTTTTT CAGGCCTCAA CTCAAACTCG TCGAGCTCCA 
GGAGGAATAA AGGAAACCAG GAAAGCAATG AGAGATTCTG ACAGTGGACT AGAAAAAATG 
GCTATTGGTC ATCATATCCA TGACCGAGCT CATGTCATTA AAAAGTCAAA GAACAAGAAG 
ACTGGAGATG AAGAGGTCAA CCAGGAGTTC ATCAATATGA ATGAAAGTGA TGCTCATGCT 
TTTGATGAGG AGTGGCAAAG TGAGGTTTTG AAGTACAAAC CAGGACGACA CAATCTAGGA 
AACACTAGAA TGAGAAGTGT TGGCCATGAG AATCCTGGCT CCCGAGAACT TAAAAGAAGG 
GAGAAACCTC AACAAAGTCC AGCCATTGAA CATGGAAGGA GATCAAATGT TTTGGGGGAC 
AAACTCCACA TCAAAGGCTC ATCTGTGAAA AGCAACAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511156.1
CDS	196..780
Translation	MDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_011512854.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512854.2

ORF Insert Sequence:

ATGGACCAAA TGGTGTCAAA TATGAGAAAC TATATGCAGA AATTAGAAAG AAACTTCGGT 
CAACTTTCAG TGGATCCAAA TGGACATTCA TTTTGTTCTT CCTCAGTTAT GACTTATTCC 
AAAATAGGAG ATGAACCGCC AAAGGTTTTT CAGGCCTCAA CTCAAACTCG TCGAGCTCCA 
GGAGGAATAA AGGAAACCAG GAAAGCAATG AGAGATTCTG ACAGTGGACT AGAAAAAATG 
GCTATTGGTC ATCATATCCA TGACCGAGCT CATGTCATTA AAAAGTCAAA GAACAAGAAG 
ACTGGAGATG AAGAGGTCAA CCAGGAGTTC ATCAATATGA ATGAAAGTGA TGCTCATGCT 
TTTGATGAGG AGTGGCAAAG TGAGGTTTTG AAGTACAAAC CAGGACGACA CAATCTAGGA 
AACACTAGAA TGAGAAGTGT TGGCCATGAG AATCCTGGCT CCCGAGAACT TAAAAGAAGG 
GAGAAACCTC AACAAAGTCC AGCCATTGAA CATGGAAGGA GATCAAATGT TTTGGGGGAC 
AAACTCCACA TCAAAGGCTC ATCTGTGAAA AGCAACAAAA AATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001195432.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182361.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20508
Clone ID Related Accession (Same CDS sequence)	NM_001195432.1 , NM_001195432.2
Accession Version	NM_001195432.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AK096889.1, AC025033.20, BX647666.1 and BU619178.1. This sequence is a reference standard in the RefSeqGene project. On Aug 31, 2013 this sequence version replaced XM_005247478.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAAAAG AAGTTCTTCA GAGAGAAGGA AAATCATACA AGTCAGAAAC TCTGATGTAC 
ATTAAAAAAG CAAGAGCATC AGAGAATAAG TTAAGTGAGT CCATTCTTGC ACACCGAGAA 
AATATGCGAC AGATGATAAG AAGTTTTTCT GAACCCTTTG GAAGAGACTT GCTCAGTATC 
TCTGATGGTA GAGGGAGAGC TCATAATCGT AGAGGACATA ATGATGGTGA AGATTCTTTG 
ACTGCAACGA GTTGTTCTCT TGTGCCTTTT GGCGATTTTG GTGGTATGCA TACAGATGTC 
AGCTCTTTCC AGACAATGGA CCAAATGGTG TCAAATATGA GAAACTATAT GCAGAAATTA 
GAAAGAAACT TCGGTCAACT TTCAGTGGAT CCAAATGGAC ATTCATTTTG TTCTTCCTCA 
GTTATGACTT ATTCCAAAAT AGGAGATGAA CCGCCAAAGG TTTTTCAGGC CTCAACTCAA 
ACTCGTCGAG CTCCAGGAGG AATAAAGGAA ACCAGGAAAG CAATGAGAGA TTCTGACAGT 
GGACTAGAAA AAATGGCTAT TGGTCATCAT ATCCATGACC GAGCTCATGT CATTAAAAAG 
TCAAAGAACA AGAAGACTGG AGATGAAGAG GTCAACCAGG AGTTCATCAA TATGAATGAA 
AGTGATGCTC ATGCTTTTGA TGAGGAGTGG CAAAGTGAGG TTTTGAAGTA CAAACCAGGA 
CGACACAATC TAGGAAACAC TAGAATGAGA AGTGTTGGCC ATGAGAATCC TGGCTCCCGA 
GAACTTAAAA GAAGGGAGAA ACCTCAACAA AGTCCAGCCA TTGAACATGG AAGGAGATCA 
AATGTTTTGG GGGACAAACT CCACATCAAA GGCTCATCTG TGAAAAGCAA CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182361.1
CDS	364..1263
Translation	MLKEVLQREGKSYKSETLMYIKKARASENKLSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001195432.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195432.1

ORF Insert Sequence:

ATGTTAAAAG AAGTTCTTCA GAGAGAAGGA AAATCATACA AGTCAGAAAC TCTGATGTAC 
ATTAAAAAAG CAAGAGCATC AGAGAATAAG TTAAGTGAGT CCATTCTTGC ACACCGAGAA 
AATATGCGAC AGATGATAAG AAGTTTTTCT GAACCCTTTG GAAGAGACTT GCTCAGTATC 
TCTGATGGTA GAGGGAGAGC TCATAATCGT AGAGGACATA ATGATGGTGA AGATTCTTTG 
ACTGCAACGA GTTGTTCTCT TGTGCCTTTT GGCGATTTTG GTGGTATGCA TACAGATGTC 
AGCTCTTTCC AGACAATGGA CCAAATGGTG TCAAATATGA GAAACTATAT GCAGAAATTA 
GAAAGAAACT TCGGTCAACT TTCAGTGGAT CCAAATGGAC ATTCATTTTG TTCTTCCTCA 
GTTATGACTT ATTCCAAAAT AGGAGATGAA CCGCCAAAGG TTTTTCAGGC CTCAACTCAA 
ACTCGTCGAG CTCCAGGAGG AATAAAGGAA ACCAGGAAAG CAATGAGAGA TTCTGACAGT 
GGACTAGAAA AAATGGCTAT TGGTCATCAT ATCCATGACC GAGCTCATGT CATTAAAAAG 
TCAAAGAACA AGAAGACTGG AGATGAAGAG GTCAACCAGG AGTTCATCAA TATGAATGAA 
AGTGATGCTC ATGCTTTTGA TGAGGAGTGG CAAAGTGAGG TTTTGAAGTA CAAACCAGGA 
CGACACAATC TAGGAAACAC TAGAATGAGA AGTGTTGGCC ATGAGAATCC TGGCTCCCGA 
GAACTTAAAA GAAGGGAGAA ACCTCAACAA AGTCCAGCCA TTGAACATGG AAGGAGATCA 
AATGTTTTGG GGGACAAACT CCACATCAAA GGCTCATCTG TGAAAAGCAA CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001195432.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182361.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu20508
Clone ID Related Accession (Same CDS sequence)	NM_001195432.1 , NM_001195432.2
Accession Version	NM_001195432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AK096889.1, AC025033.20 and BX647666.1. On Jun 2, 2019 this sequence version replaced NM_001195432.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (3). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK096889.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTAAAAG AAGTTCTTCA GAGAGAAGGA AAATCATACA AGTCAGAAAC TCTGATGTAC 
ATTAAAAAAG CAAGAGCATC AGAGAATAAG TTAAGTGAGT CCATTCTTGC ACACCGAGAA 
AATATGCGAC AGATGATAAG AAGTTTTTCT GAACCCTTTG GAAGAGACTT GCTCAGTATC 
TCTGATGGTA GAGGGAGAGC TCATAATCGT AGAGGACATA ATGATGGTGA AGATTCTTTG 
ACTGCAACGA GTTGTTCTCT TGTGCCTTTT GGCGATTTTG GTGGTATGCA TACAGATGTC 
AGCTCTTTCC AGACAATGGA CCAAATGGTG TCAAATATGA GAAACTATAT GCAGAAATTA 
GAAAGAAACT TCGGTCAACT TTCAGTGGAT CCAAATGGAC ATTCATTTTG TTCTTCCTCA 
GTTATGACTT ATTCCAAAAT AGGAGATGAA CCGCCAAAGG TTTTTCAGGC CTCAACTCAA 
ACTCGTCGAG CTCCAGGAGG AATAAAGGAA ACCAGGAAAG CAATGAGAGA TTCTGACAGT 
GGACTAGAAA AAATGGCTAT TGGTCATCAT ATCCATGACC GAGCTCATGT CATTAAAAAG 
TCAAAGAACA AGAAGACTGG AGATGAAGAG GTCAACCAGG AGTTCATCAA TATGAATGAA 
AGTGATGCTC ATGCTTTTGA TGAGGAGTGG CAAAGTGAGG TTTTGAAGTA CAAACCAGGA 
CGACACAATC TAGGAAACAC TAGAATGAGA AGTGTTGGCC ATGAGAATCC TGGCTCCCGA 
GAACTTAAAA GAAGGGAGAA ACCTCAACAA AGTCCAGCCA TTGAACATGG AAGGAGATCA 
AATGTTTTGG GGGACAAACT CCACATCAAA GGCTCATCTG TGAAAAGCAA CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182361.1
CDS	334..1233
Translation	MLKEVLQREGKSYKSETLMYIKKARASENKLSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001195432.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195432.2

ORF Insert Sequence:

ATGTTAAAAG AAGTTCTTCA GAGAGAAGGA AAATCATACA AGTCAGAAAC TCTGATGTAC 
ATTAAAAAAG CAAGAGCATC AGAGAATAAG TTAAGTGAGT CCATTCTTGC ACACCGAGAA 
AATATGCGAC AGATGATAAG AAGTTTTTCT GAACCCTTTG GAAGAGACTT GCTCAGTATC 
TCTGATGGTA GAGGGAGAGC TCATAATCGT AGAGGACATA ATGATGGTGA AGATTCTTTG 
ACTGCAACGA GTTGTTCTCT TGTGCCTTTT GGCGATTTTG GTGGTATGCA TACAGATGTC 
AGCTCTTTCC AGACAATGGA CCAAATGGTG TCAAATATGA GAAACTATAT GCAGAAATTA 
GAAAGAAACT TCGGTCAACT TTCAGTGGAT CCAAATGGAC ATTCATTTTG TTCTTCCTCA 
GTTATGACTT ATTCCAAAAT AGGAGATGAA CCGCCAAAGG TTTTTCAGGC CTCAACTCAA 
ACTCGTCGAG CTCCAGGAGG AATAAAGGAA ACCAGGAAAG CAATGAGAGA TTCTGACAGT 
GGACTAGAAA AAATGGCTAT TGGTCATCAT ATCCATGACC GAGCTCATGT CATTAAAAAG 
TCAAAGAACA AGAAGACTGG AGATGAAGAG GTCAACCAGG AGTTCATCAA TATGAATGAA 
AGTGATGCTC ATGCTTTTGA TGAGGAGTGG CAAAGTGAGG TTTTGAAGTA CAAACCAGGA 
CGACACAATC TAGGAAACAC TAGAATGAGA AGTGTTGGCC ATGAGAATCC TGGCTCCCGA 
GAACTTAAAA GAAGGGAGAA ACCTCAACAA AGTCCAGCCA TTGAACATGG AAGGAGATCA 
AATGTTTTGG GGGACAAACT CCACATCAAA GGCTCATCTG TGAAAAGCAA CAAAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001195434.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182363.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu32246
Clone ID Related Accession (Same CDS sequence)	NM_001195434.1 , NM_001195434.2 , NM_001369785.1 , XM_005247480.2 , XM_011512852.2 , XM_011512852.3
Accession Version	NM_001195434.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AK056948.1, BX647666.1 and BU619178.1. Transcript Variant: This variant (6) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 5, which is shorter than isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182363.1
CDS	330..1106
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001195434.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195434.1

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001195434.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182363.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu32246
Clone ID Related Accession (Same CDS sequence)	NM_001195434.1 , NM_001195434.2 , NM_001369785.1 , XM_005247480.2 , XM_011512852.2 , XM_011512852.3
Accession Version	NM_001195434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK056948.1 and BX647666.1. On Jun 2, 2019 this sequence version replaced NM_001195434.1. Transcript Variant: This variant (6) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 5, which is shorter than isoform 3. Variants 6 and 11 encode the same isoform (5). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK056948.1, SRR5189655.45453.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2149178 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182363.1
CDS	300..1076
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001195434.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195434.2

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001369785.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356714.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 11, mRNA.

Price & Availability↑

CloneID	OHu32246
Clone ID Related Accession (Same CDS sequence)	NM_001195434.1 , NM_001195434.2 , NM_001369785.1 , XM_005247480.2 , XM_011512852.2 , XM_011512852.3
Accession Version	NM_001369785.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106707.9 and AC025033.20. On Apr 18, 2019 this sequence version replaced XM_005247480.2. Transcript Variant: Variants 6 and 11 encode the same isoform (5). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.56903.1, SRR5189655.172509.1 [ECO:0000332] ##Evidence-Data-END##

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356714.1
CDS	227..1003
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001369785.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369785.1

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001195433.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182362.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu32345
Clone ID Related Accession (Same CDS sequence)	NM_001195433.1 , NM_001195433.2
Accession Version	NM_001195433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AY848700.1, BX647666.1 and BU619178.1. Transcript Variant: This variant (5) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 4, which is shorter than isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY848700.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCATAAAG GAAACCAGGA AAGCAATGAG AGATTCTGAC 
AGTGGACTAG AAAAAATGGC TATTGGTCAT CATATCCATG ACCGAGCTCA TGTCATTAAA 
AAGTCAAAGA ACAAGAAGAC TGGAGATGAA GAGGTCAACC AGGAGTTCAT CAATATGAAT 
GAAAGTGATG CTCATGCTTT TGATGAGGAG TGGCAAAGTG AGGTTTTGAA GTACAAACCA 
GGACGACACA ATCTAGGAAA CACTAGAATG AGAAGTGTTG GCCATGAGAA TCCTGGCTCC 
CGAGAACTTA AAAGAAGGGA GAAACCTCAA CAAAGTCCAG CCATTGAACA TGGAAGGAGA 
TCAAATGTTT TGGGGGACAA ACTCCACATC AAAGGCTCAT CTGTGAAAAG CAACAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182362.1
CDS	330..932
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001195433.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195433.1

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCATAAAG GAAACCAGGA AAGCAATGAG AGATTCTGAC 
AGTGGACTAG AAAAAATGGC TATTGGTCAT CATATCCATG ACCGAGCTCA TGTCATTAAA 
AAGTCAAAGA ACAAGAAGAC TGGAGATGAA GAGGTCAACC AGGAGTTCAT CAATATGAAT 
GAAAGTGATG CTCATGCTTT TGATGAGGAG TGGCAAAGTG AGGTTTTGAA GTACAAACCA 
GGACGACACA ATCTAGGAAA CACTAGAATG AGAAGTGTTG GCCATGAGAA TCCTGGCTCC 
CGAGAACTTA AAAGAAGGGA GAAACCTCAA CAAAGTCCAG CCATTGAACA TGGAAGGAGA 
TCAAATGTTT TGGGGGACAA ACTCCACATC AAAGGCTCAT CTGTGAAAAG CAACAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001195433.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182362.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu32345
Clone ID Related Accession (Same CDS sequence)	NM_001195433.1 , NM_001195433.2
Accession Version	NM_001195433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455518.1, AY848700.1 and BX647666.1. On Jun 2, 2019 this sequence version replaced NM_001195433.1. Transcript Variant: This variant (5) differs in the 5' UTR and has multiple differences in the coding region, but maintains the reading frame compared to variant 4. This variant encodes isoform 4, which is shorter than isoform 3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY848700.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968540, SAMEA1968968 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCATAAAG GAAACCAGGA AAGCAATGAG AGATTCTGAC 
AGTGGACTAG AAAAAATGGC TATTGGTCAT CATATCCATG ACCGAGCTCA TGTCATTAAA 
AAGTCAAAGA ACAAGAAGAC TGGAGATGAA GAGGTCAACC AGGAGTTCAT CAATATGAAT 
GAAAGTGATG CTCATGCTTT TGATGAGGAG TGGCAAAGTG AGGTTTTGAA GTACAAACCA 
GGACGACACA ATCTAGGAAA CACTAGAATG AGAAGTGTTG GCCATGAGAA TCCTGGCTCC 
CGAGAACTTA AAAGAAGGGA GAAACCTCAA CAAAGTCCAG CCATTGAACA TGGAAGGAGA 
TCAAATGTTT TGGGGGACAA ACTCCACATC AAAGGCTCAT CTGTGAAAAG CAACAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182362.1
CDS	300..902
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTHTDVSSFQTMDQMVSNMRNYMQKLERNFIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001195433.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195433.2

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCATAAAG GAAACCAGGA AAGCAATGAG AGATTCTGAC 
AGTGGACTAG AAAAAATGGC TATTGGTCAT CATATCCATG ACCGAGCTCA TGTCATTAAA 
AAGTCAAAGA ACAAGAAGAC TGGAGATGAA GAGGTCAACC AGGAGTTCAT CAATATGAAT 
GAAAGTGATG CTCATGCTTT TGATGAGGAG TGGCAAAGTG AGGTTTTGAA GTACAAACCA 
GGACGACACA ATCTAGGAAA CACTAGAATG AGAAGTGTTG GCCATGAGAA TCCTGGCTCC 
CGAGAACTTA AAAGAAGGGA GAAACCTCAA CAAAGTCCAG CCATTGAACA TGGAAGGAGA 
TCAAATGTTT TGGGGGACAA ACTCCACATC AAAGGCTCAT CTGTGAAAAG CAACAAAAAA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001369783.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356712.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 9, mRNA.

Price & Availability↑

CloneID	OHu37959
Clone ID Related Accession (Same CDS sequence)	XM_005247479.5 , XM_005247479.4 , NM_001369783.1
Accession Version	NM_001369783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106707.9, KU178187.1 and AC025033.20. On Apr 18, 2019 this sequence version replaced XM_005247479.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189655.181954.1, SRR5189655.221586.1 [ECO:0000332] ##Evidence-Data-END##

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTGCAAC GAGTTGTTCT CTTGTGCCTT TTGGCGATTT TGGTGGTATG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356712.1
CDS	108..959
Translation	MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001369783.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369783.1

ORF Insert Sequence:

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTGCAAC GAGTTGTTCT CTTGTGCCTT TTGGCGATTT TGGTGGTATG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( NM_001369781.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001356710.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu37960
Clone ID Related Accession (Same CDS sequence)	NM_001369781.1 , XM_006713645.2
Accession Version	NM_001369781.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC106707.9 and AC025033.20. On Apr 18, 2019 this sequence version replaced XM_006713645.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.118474.1, SRR1803614.31099.1 [ECO:0000332] ##Evidence-Data-END##

ATGACCCCTT CTTCTCGTGA GTTACGGGAG CCAGGAGTCC GGGGTCGCGC GGGAATTAAG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001356710.1
CDS	139..810
Translation	MTPSSRELREPGVRGRAGIKHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	NM_001369781.1
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001369781.1

ORF Insert Sequence:

ATGACCCCTT CTTCTCGTGA GTTACGGGAG CCAGGAGTCC GGGGTCGCGC GGGAATTAAG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_005247480.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247537.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu32246
Clone ID Related Accession (Same CDS sequence)	NM_001195434.1 , NM_001195434.2 , NM_001369785.1 , XM_005247480.2 , XM_011512852.2 , XM_011512852.3
Accession Version	XM_005247480.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005247480.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247537.1
CDS	199..975
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_005247480.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247480.2

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_011512852.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511154.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu32246
Clone ID Related Accession (Same CDS sequence)	NM_001195434.1 , NM_001195434.2 , NM_001369785.1 , XM_005247480.2 , XM_011512852.2 , XM_011512852.3
Accession Version	XM_011512852.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512852.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511154.1
CDS	288..1064
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_011512852.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512852.2

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_011512852.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511154.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu32246
Clone ID Related Accession (Same CDS sequence)	NM_001195434.1 , NM_001195434.2 , NM_001369785.1 , XM_005247480.2 , XM_011512852.2 , XM_011512852.3
Accession Version	XM_011512852.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011512852.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511154.1
CDS	287..1063
Translation	MRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_011512852.3
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512852.3

ORF Insert Sequence:

ATGCGACAGA TGATAAGAAG TTTTTCTGAA CCCTTTGGAA GAGACTTGCT CAGTATCTCT 
GATGGTAGAG GGAGAGCTCA TAATCGTAGA GGACATAATG ATGGTGAAGA TTCTTTGACT 
GCAACGAGTT GTTCTCTTGT GCCTTTTGGC GATTTTGGTG GTATGCATAC AGATGTCAGC 
TCTTTCCAGA CAATGGACCA AATGGTGTCA AATATGAGAA ACTATATGCA GAAATTAGAA 
AGAAACTTCG GTCAACTTTC AGTGGATCCA AATGGACATT CATTTTGTTC TTCCTCAGTT 
ATGACTTATT CCAAAATAGG AGATGAACCG CCAAAGGTTT TTCAGGCCTC AACTCAAACT 
CGTCGAGCTC CAGGAGGAAT AAAGGAAACC AGGAAAGCAA TGAGAGATTC TGACAGTGGA 
CTAGAAAAAA TGGCTATTGG TCATCATATC CATGACCGAG CTCATGTCAT TAAAAAGTCA 
AAGAACAAGA AGACTGGAGA TGAAGAGGTC AACCAGGAGT TCATCAATAT GAATGAAAGT 
GATGCTCATG CTTTTGATGA GGAGTGGCAA AGTGAGGTTT TGAAGTACAA ACCAGGACGA 
CACAATCTAG GAAACACTAG AATGAGAAGT GTTGGCCATG AGAATCCTGG CTCCCGAGAA 
CTTAAAAGAA GGGAGAAACC TCAACAAAGT CCAGCCATTG AACATGGAAG GAGATCAAAT 
GTTTTGGGGG ACAAACTCCA CATCAAAGGC TCATCTGTGA AAAGCAACAA AAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_005247479.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247536.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu37959
Clone ID Related Accession (Same CDS sequence)	XM_005247479.5 , XM_005247479.4 , NM_001369783.1
Accession Version	XM_005247479.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005247479.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTGCAAC GAGTTGTTCT CTTGTGCCTT TTGGCGATTT TGGTGGTATG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247536.1
CDS	139..990
Translation	MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_005247479.5
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247479.5

ORF Insert Sequence:

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTGCAAC GAGTTGTTCT CTTGTGCCTT TTGGCGATTT TGGTGGTATG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_005247479.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005247536.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu37959
Clone ID Related Accession (Same CDS sequence)	XM_005247479.5 , XM_005247479.4 , NM_001369783.1
Accession Version	XM_005247479.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005247479.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTGCAAC GAGTTGTTCT CTTGTGCCTT TTGGCGATTT TGGTGGTATG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005247536.1
CDS	140..991
Translation	MFRMLNSSFEDDPFFSESILAHRENMRQMIRSFSEPFGRDLLSISDGRGRAHNRRGHNDGEDSLTATSCSLVPFGDFGGMHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_005247479.4
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005247479.4

ORF Insert Sequence:

ATGTTCAGGA TGCTGAACAG CAGTTTTGAG GATGACCCCT TCTTCTCTGA GTCCATTCTT 
GCACACCGAG AAAATATGCG ACAGATGATA AGAAGTTTTT CTGAACCCTT TGGAAGAGAC 
TTGCTCAGTA TCTCTGATGG TAGAGGGAGA GCTCATAATC GTAGAGGACA TAATGATGGT 
GAAGATTCTT TGACTGCAAC GAGTTGTTCT CTTGTGCCTT TTGGCGATTT TGGTGGTATG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MLF1 ( XM_006713645.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713708.1 Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu37960
Clone ID Related Accession (Same CDS sequence)	NM_001369781.1 , XM_006713645.2
Accession Version	XM_006713645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 672bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	myeloid leukemia factor 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_006713645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCCCTT CTTCTCGTGA GTTACGGGAG CCAGGAGTCC GGGGTCGCGC GGGAATTAAG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713708.1
CDS	111..782
Translation	MTPSSRELREPGVRGRAGIKHTDVSSFQTMDQMVSNMRNYMQKLERNFGQLSVDPNGHSFCSSSVMTYSKIGDEPPKVFQASTQTRRAPGGIKETRKAMRDSDSGLEKMAIGHHIHDRAHVIKKSKNKKTGDEEVNQEFINMNESDAHAFDEEWQSEVLKYKPGRHNLGNTRMRSVGHENPGSRELKRREKPQQSPAIEHGRRSNVLGDKLHIKGSSVKSNKK

Target ORF information:

RefSeq Version	XM_006713645.2
Organism	Homo sapiens(human)
Definition	Homo sapiens myeloid leukemia factor 1 (MLF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713645.2

ORF Insert Sequence:

ATGACCCCTT CTTCTCGTGA GTTACGGGAG CCAGGAGTCC GGGGTCGCGC GGGAATTAAG 
CATACAGATG TCAGCTCTTT CCAGACAATG GACCAAATGG TGTCAAATAT GAGAAACTAT 
ATGCAGAAAT TAGAAAGAAA CTTCGGTCAA CTTTCAGTGG ATCCAAATGG ACATTCATTT 
TGTTCTTCCT CAGTTATGAC TTATTCCAAA ATAGGAGATG AACCGCCAAA GGTTTTTCAG 
GCCTCAACTC AAACTCGTCG AGCTCCAGGA GGAATAAAGG AAACCAGGAA AGCAATGAGA 
GATTCTGACA GTGGACTAGA AAAAATGGCT ATTGGTCATC ATATCCATGA CCGAGCTCAT 
GTCATTAAAA AGTCAAAGAA CAAGAAGACT GGAGATGAAG AGGTCAACCA GGAGTTCATC 
AATATGAATG AAAGTGATGC TCATGCTTTT GATGAGGAGT GGCAAAGTGA GGTTTTGAAG 
TACAAACCAG GACGACACAA TCTAGGAAAC ACTAGAATGA GAAGTGTTGG CCATGAGAAT 
CCTGGCTCCC GAGAACTTAA AAGAAGGGAG AAACCTCAAC AAAGTCCAGC CATTGAACAT 
GGAAGGAGAT CAAATGTTTT GGGGGACAAA CTCCACATCA AAGGCTCATC TGTGAAAAGC 
AACAAAAAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Myeloid Leukemia Factor Acts in a Chaperone Complex to Regulate Transcription Factor Stability and Gene Expression.
Journal of molecular biology429(13)2093-2107(2017 Jun)
Dyer JO,Dutta A,Gogol M,Weake VM,Dialynas G,Wu X,Seidel C,Zhang Y,Florens L,Washburn MP,Abmayr SM,Workman JL

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Common variants upstream of MLF1 at 3q25 and within CPZ at 4p16 associated with neuroblastoma.
PLoS genetics13(5)e1006787(2017 May)
McDaniel LD,Conkrite KL,Chang X,Capasso M,Vaksman Z,Oldridge DA,Zachariou A,Horn M,Diamond M,Hou C,Iolascon A,Hakonarson H,Rahman N,Devoto M,Diskin SJ

MLF1 is a proapoptotic antagonist of HOP complex-mediated survival.
Biochimica et biophysica acta1864(4)719-727(2017 04)
Sun Y,Chao JR,Xu W,Pourpak A,Boyd K,Moshiach S,Qi GY,Fu A,Shao HR,Pounds S,Morris SW

Modulation of mutant Huntingtin aggregates and toxicity by human myeloid leukemia factors.
The international journal of biochemistry & cell biology821-9(2017 01)
Banerjee M,Datta M,Bhattacharyya NP

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MLF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: