ASCL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ASCL1
Entrez Gene ID	429
Full Name	achaete-scute family bHLH transcription factor 1
Synonyms	ASH1,HASH1,MASH1,bHLHa46
General protein information	Preferred Names achaete-scute family bHLH transcription factor 1 Names achaete-scute homolog 1 ASH-1 achaete scute protein achaete-scute complex homolog 1 achaete-scute complex-like 1 class A basic helix-loop-helix protein 46
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q23.2
Summary	This gene encodes a member of the basic helix-loop-helix (BHLH) family of transcription factors. The protein activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. This protein plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. Mutations in this gene may contribute to the congenital central hypoventilation syndrome (CCHS) phenotype in rare cases. [provided by RefSeq, Jul 2008].
Disorder MIM:	100790
Disorder Html:	Central hypoventilation syndrome, congenital, 209880 (3); Haddad syndrome, 209880 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004316.4	NP_004307.2	achaete-scute homolog 1
NM_004316.3	NP_004307.2	achaete-scute homolog 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	ASCL1	XP_002687686.1
Rattus norvegicus (Norway rat)	Ascl1	NP_071779.1
Xenopus tropicalis (tropical clawed frog)	ascl1	XP_002944648.2
Pan troglodytes (chimpanzee)	ASCL1	XP_003954517.1
	ASCL1	XP_001094978.1
Canis lupus familiaris (dog)	ASCL1	XP_539745.3
Mus musculus (house mouse)	Ascl1	NP_032579.2
Danio rerio (zebrafish)	ascl1a	NP_571294.1
Homo sapiens (human)	ASCL1	NP_004307.2

Gene Ontology
Bibliography

Related articles in PubMed

ASCL1 Reorganizes Chromatin to Direct Neuronal Fate and Suppress Tumorigenicity of Glioblastoma Stem Cells.
Park NI, Guilhamon P, Desai K, McAdam RF, Langille E, O'Connor M, Lan X, Whetstone H, Coutinho FJ, Vanner RJ, Ling E, Prinos P, Lee L, Selvadurai H, Atwal G, Kushida M, Clarke ID, Voisin V, Cusimano MD, Bernstein M, Das S, Bader G, Arrowsmith CH, Angers S, Huang X, Lupien M, Dirks PB
Cell stem cell21(2)209-224.e7(2017 Aug)

System analysis identifies distinct and common functional networks governed by transcription factor ASCL1, in glioma and small cell lung cancer.
Donakonda S, Sinha S, Dighe SN, Rao MRS
Molecular bioSystems13(8)1481-1494(2017 Jul)

Hypermethylation of adjacent CpG sites is negatively correlated with the expression of lineage oncogene ASCL1 in pulmonary neuroendocrine tumors.
Truong N, Chun SM, Kim TI, Suh YA, Jang SJ
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317706225(2017 Jun)

Achaete-scute complex homologue-1 promotes development of laryngocarcinoma via facilitating the epithelial-mesenchymal transformation.
Ma H, Du X, Zhang S, Wang Q, Yin Y, Qiu X, Da P, Yue H, Wu H, Xu F
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317705752(2017 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we found that CpG_6.7.8 of the achaete-scute homolog 1 CpG island is frequently hypermethylated in early-stage pulmonary neuroendocrine tumors, and this aberrant hypermethylation is negatively correlated with achaete-scute homolog 1 expression in this tumor spectrum
Title: Hypermethylation of adjacent CpG sites is negatively correlated with the expression of lineage oncogene ASCL1 in pulmonary neuroendocrine tumors.

knocking down achaete-scute complex homologue-1 expression could significantly suppress the proliferation, migration, and invasion of laryngeal carcinoma cell in vitro and disorder epithelial-mesenchymal transformation-associated protein expression.
Title: Achaete-scute complex homologue-1 promotes development of laryngocarcinoma via facilitating the epithelial-mesenchymal transformation.

the first comprehensive high-resolution information on the structural propensities and conformational dynamics of Ascl1, is reported.
Title: Fragment-Based NMR Study of the Conformational Dynamics in the bHLH Transcription Factor Ascl1.

ASCL1 expression may determine the cell behaviors of SCLC partly through modifying EMT phenotypes.
Title: Small cell lung cancer, an epithelial to mesenchymal transition (EMT)-like cancer: significance of inactive Notch signaling and expression of achaete-scute complex homologue 1.

Results indicate transcription factor Ascl1 protein functionalized with intracellular protein delivery technology (Ascl1-IPTD) as a powerful tool for engineering neural tissue from pluripotent stem cells.
Title: Functionalizing Ascl1 with Novel Intracellular Protein Delivery Technology for Promoting Neuronal Differentiation of Human Induced Pluripotent Stem Cells.

The following ASCL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASCL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19355	NM_004316.4 Latest version!	Homo sapiens achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu19355	NM_004316.3	Homo sapiens achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ASCL1 ( NM_004316.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004307.2 Homo sapiens achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.

Price & Availability↑

CloneID	OHu19355
Clone ID Related Accession (Same CDS sequence)	NM_004316.4 , NM_004316.3
Accession Version	NM_004316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	achaete-scute homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA005289.1, BC004425.1, CF454566.1, AC026108.29 and AW071836.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_004316.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC001638.2, L08424.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000266744.4/ ENSP00000266744.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAAGCT CTGCCAAGAT GGAGAGCGGC GGCGCCGGCC AGCAGCCCCA GCCGCAGCCC 
CAGCAGCCCT TCCTGCCGCC CGCAGCCTGT TTCTTTGCCA CGGCCGCAGC CGCGGCGGCC 
GCAGCCGCCG CAGCGGCAGC GCAGAGCGCG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGGCGC CGCAGCTGAG ACCGGCGGCC GACGGCCAGC CCTCAGGGGG CGGTCACAAG 
TCAGCGCCCA AGCAAGTCAA GCGACAGCGC TCGTCTTCGC CCGAACTGAT GCGCTGCAAA 
CGCCGGCTCA ACTTCAGCGG CTTTGGCTAC AGCCTGCCGC AGCAGCAGCC GGCCGCCGTG 
GCGCGCCGCA ACGAGCGCGA GCGCAACCGC GTCAAGTTGG TCAACCTGGG CTTTGCCACC 
CTTCGGGAGC ACGTCCCCAA CGGCGCGGCC AACAAGAAGA TGAGTAAGGT GGAGACACTG 
CGCTCGGCGG TCGAGTACAT CCGCGCGCTG CAGCAGCTGC TGGACGAGCA TGACGCGGTG 
AGCGCCGCCT TCCAGGCAGG CGTCCTGTCG CCCACCATCT CCCCCAACTA CTCCAACGAC 
TTGAACTCCA TGGCCGGCTC GCCGGTCTCA TCCTACTCGT CGGACGAGGG CTCTTACGAC 
CCGCTCAGCC CCGAGGAGCA GGAGCTTCTC GACTTCACCA ACTGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004307.2
CDS	572..1282
Translation	MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF

Target ORF information:

RefSeq Version	NM_004316.4
Organism	Homo sapiens(human)
Definition	Homo sapiens achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004316.4

ORF Insert Sequence:

ATGGAAAGCT CTGCCAAGAT GGAGAGCGGC GGCGCCGGCC AGCAGCCCCA GCCGCAGCCC 
CAGCAGCCCT TCCTGCCGCC CGCAGCCTGT TTCTTTGCCA CGGCCGCAGC CGCGGCGGCC 
GCAGCCGCCG CAGCGGCAGC GCAGAGCGCG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGGCGC CGCAGCTGAG ACCGGCGGCC GACGGCCAGC CCTCAGGGGG CGGTCACAAG 
TCAGCGCCCA AGCAAGTCAA GCGACAGCGC TCGTCTTCGC CCGAACTGAT GCGCTGCAAA 
CGCCGGCTCA ACTTCAGCGG CTTTGGCTAC AGCCTGCCGC AGCAGCAGCC GGCCGCCGTG 
GCGCGCCGCA ACGAGCGCGA GCGCAACCGC GTCAAGTTGG TCAACCTGGG CTTTGCCACC 
CTTCGGGAGC ACGTCCCCAA CGGCGCGGCC AACAAGAAGA TGAGTAAGGT GGAGACACTG 
CGCTCGGCGG TCGAGTACAT CCGCGCGCTG CAGCAGCTGC TGGACGAGCA TGACGCGGTG 
AGCGCCGCCT TCCAGGCAGG CGTCCTGTCG CCCACCATCT CCCCCAACTA CTCCAACGAC 
TTGAACTCCA TGGCCGGCTC GCCGGTCTCA TCCTACTCGT CGGACGAGGG CTCTTACGAC 
CCGCTCAGCC CCGAGGAGCA GGAGCTTCTC GACTTCACCA ACTGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ASCL1 ( NM_004316.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004307.2 Homo sapiens achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.

Price & Availability↑

CloneID	OHu19355
Clone ID Related Accession (Same CDS sequence)	NM_004316.4 , NM_004316.3
Accession Version	NM_004316.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-11
Organism	Homo sapiens(human)
Product	achaete-scute homolog 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA005289.1, BC004425.1, CF454566.1, AC026108.29 and AW071836.1. This sequence is a reference standard in the RefSeqGene project. On Jun 13, 2008 this sequence version replaced NM_004316.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK290539.1, BC001638.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA2142586 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAGCT CTGCCAAGAT GGAGAGCGGC GGCGCCGGCC AGCAGCCCCA GCCGCAGCCC 
CAGCAGCCCT TCCTGCCGCC CGCAGCCTGT TTCTTTGCCA CGGCCGCAGC CGCGGCGGCC 
GCAGCCGCCG CAGCGGCAGC GCAGAGCGCG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGGCGC CGCAGCTGAG ACCGGCGGCC GACGGCCAGC CCTCAGGGGG CGGTCACAAG 
TCAGCGCCCA AGCAAGTCAA GCGACAGCGC TCGTCTTCGC CCGAACTGAT GCGCTGCAAA 
CGCCGGCTCA ACTTCAGCGG CTTTGGCTAC AGCCTGCCGC AGCAGCAGCC GGCCGCCGTG 
GCGCGCCGCA ACGAGCGCGA GCGCAACCGC GTCAAGTTGG TCAACCTGGG CTTTGCCACC 
CTTCGGGAGC ACGTCCCCAA CGGCGCGGCC AACAAGAAGA TGAGTAAGGT GGAGACACTG 
CGCTCGGCGG TCGAGTACAT CCGCGCGCTG CAGCAGCTGC TGGACGAGCA TGACGCGGTG 
AGCGCCGCCT TCCAGGCAGG CGTCCTGTCG CCCACCATCT CCCCCAACTA CTCCAACGAC 
TTGAACTCCA TGGCCGGCTC GCCGGTCTCA TCCTACTCGT CGGACGAGGG CTCTTACGAC 
CCGCTCAGCC CCGAGGAGCA GGAGCTTCTC GACTTCACCA ACTGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004307.2
CDS	572..1282
Translation	MESSAKMESGGAGQQPQPQPQQPFLPPAACFFATAAAAAAAAAAAAAQSAQQQQQQQQQQQQAPQLRPAADGQPSGGGHKSAPKQVKRQRSSSPELMRCKRRLNFSGFGYSLPQQQPAAVARRNERERNRVKLVNLGFATLREHVPNGAANKKMSKVETLRSAVEYIRALQQLLDEHDAVSAAFQAGVLSPTISPNYSNDLNSMAGSPVSSYSSDEGSYDPLSPEEQELLDFTNWF

Target ORF information:

RefSeq Version	NM_004316.3
Organism	Homo sapiens(human)
Definition	Homo sapiens achaete-scute family bHLH transcription factor 1 (ASCL1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004316.3

ORF Insert Sequence:

ATGGAAAGCT CTGCCAAGAT GGAGAGCGGC GGCGCCGGCC AGCAGCCCCA GCCGCAGCCC 
CAGCAGCCCT TCCTGCCGCC CGCAGCCTGT TTCTTTGCCA CGGCCGCAGC CGCGGCGGCC 
GCAGCCGCCG CAGCGGCAGC GCAGAGCGCG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
CAGCAGGCGC CGCAGCTGAG ACCGGCGGCC GACGGCCAGC CCTCAGGGGG CGGTCACAAG 
TCAGCGCCCA AGCAAGTCAA GCGACAGCGC TCGTCTTCGC CCGAACTGAT GCGCTGCAAA 
CGCCGGCTCA ACTTCAGCGG CTTTGGCTAC AGCCTGCCGC AGCAGCAGCC GGCCGCCGTG 
GCGCGCCGCA ACGAGCGCGA GCGCAACCGC GTCAAGTTGG TCAACCTGGG CTTTGCCACC 
CTTCGGGAGC ACGTCCCCAA CGGCGCGGCC AACAAGAAGA TGAGTAAGGT GGAGACACTG 
CGCTCGGCGG TCGAGTACAT CCGCGCGCTG CAGCAGCTGC TGGACGAGCA TGACGCGGTG 
AGCGCCGCCT TCCAGGCAGG CGTCCTGTCG CCCACCATCT CCCCCAACTA CTCCAACGAC 
TTGAACTCCA TGGCCGGCTC GCCGGTCTCA TCCTACTCGT CGGACGAGGG CTCTTACGAC 
CCGCTCAGCC CCGAGGAGCA GGAGCTTCTC GACTTCACCA ACTGGTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ASCL1 Reorganizes Chromatin to Direct Neuronal Fate and Suppress Tumorigenicity of Glioblastoma Stem Cells.
Cell stem cell21(2)209-224.e7(2017 Aug)
Park NI,Guilhamon P,Desai K,McAdam RF,Langille E,O'Connor M,Lan X,Whetstone H,Coutinho FJ,Vanner RJ,Ling E,Prinos P,Lee L,Selvadurai H,Atwal G,Kushida M,Clarke ID,Voisin V,Cusimano MD,Bernstein M,Das S,Bader G,Arrowsmith CH,Angers S,Huang X,Lupien M,Dirks PB

System analysis identifies distinct and common functional networks governed by transcription factor ASCL1, in glioma and small cell lung cancer.
Molecular bioSystems13(8)1481-1494(2017 Jul)
Donakonda S,Sinha S,Dighe SN,Rao MRS

Hypermethylation of adjacent CpG sites is negatively correlated with the expression of lineage oncogene ASCL1 in pulmonary neuroendocrine tumors.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317706225(2017 Jun)
Truong N,Chun SM,Kim TI,Suh YA,Jang SJ

Achaete-scute complex homologue-1 promotes development of laryngocarcinoma via facilitating the epithelial-mesenchymal transformation.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine39(6)1010428317705752(2017 Jun)
Ma H,Du X,Zhang S,Wang Q,Yin Y,Qiu X,Da P,Yue H,Wu H,Xu F

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ASCL1 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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