MELTF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MELTF
Entrez Gene ID	4241
Full Name	melanotransferrin
Synonyms	CD228,MAP97,MFI2,MTF1,MTf
General protein information	Preferred Names melanotransferrin Names melanotransferrin antigen p97 (melanoma associated) identified by monoclonal antibodies 133.2 and 96.5 melanoma-associated antigen p97 membrane-bound transferrin-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q29
Summary	The protein encoded by this gene is a cell-surface glycoprotein found on melanoma cells. The protein shares sequence similarity and iron-binding properties with members of the transferrin superfamily. The importance of the iron binding function has not yet been identified. This gene resides in the same region of chromosome 3 as members of the transferrin superfamily. Alternative splicing results in two transcript variants. [provided by RefSeq, Jul 2008].
Disorder MIM:	155750

mRNA and Protein(s)

mRNA	Protein	Name
NM_005929.5	NP_005920.2	melanotransferrin isoform 1 preproprotein
NM_033316.3	NP_201573.1	melanotransferrin isoform 2 precursor
XM_011512850.2	XP_011511152.1	melanotransferrin isoform X1
XM_011512851.2	XP_011511153.1	melanotransferrin isoform X3
NM_005929.6	NP_005920.2	melanotransferrin isoform 1 preproprotein
NM_033316.4	NP_201573.1	melanotransferrin isoform 2 precursor
XM_006713643.3	XP_006713706.2	melanotransferrin isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MFI2	NP_001179241.1
Mus musculus (house mouse)	Mfi2	NP_038928.1
Danio rerio (zebrafish)	mfi2	XP_694299.4
Xenopus tropicalis (tropical clawed frog)	mfi2	XP_002937877.2
Canis lupus familiaris (dog)	MFI2	XP_005639711.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005003	XP_315107.4
Rattus norvegicus (Norway rat)	Mfi2	NP_001099342.1
Homo sapiens (human)	MFI2	NP_005920.2
Drosophila melanogaster (fruit fly)	Tsf2	NP_524044.1
Pan troglodytes (chimpanzee)	MFI2	XP_003310242.2
	MFI2	XP_001096034.2
Gallus gallus (chicken)	MFI2	NP_990538.1

Gene Ontology
Bibliography

Related articles in PubMed

Discovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics.
Shin J, Kim HJ, Kim G, Song M, Woo SJ, Lee ST, Kim H, Lee C
Journal of proteome research13(11)4919-31(2014 Nov)

PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesis.
Zhang K, Waxman DJ
Molecular cancer9319(2010 Dec)

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Buschow SI, van Balkom BW, Aalberts M, Heck AJ, Wauben M, Stoorvogel W
Immunology and cell biology88(8)851-6(2010 Nov-Dec)

Meckel-Gruber syndrome protein MKS3 is required for endoplasmic reticulum-associated degradation of surfactant protein C.
Wang M, Bridges JP, Na CL, Xu Y, Weaver TE
The Journal of biological chemistry284(48)33377-83(2009 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Melanotransferrin is a serological marker of colorectal cancer by secretome analysis and quantitative proteomics.
Title: Discovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics.

PC3 tumors are sustained by a small number of tumor-initiating cells with stem-like characteristics, including strong self-renewal and pro-angiogenic capability and marked by the expression pattern FAM65Bhigh/MFI2low/LEF1low.
Title: PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesis.

Biochemical and spectroscopic studies of MTF1 are reported.
Title: Biochemical and spectroscopic studies of human melanotransferrin (MTf): electron-paramagnetic resonance evidence for a difference between the iron-binding site of MTf and other transferrins.

this review discusses the studies that have demonstrated that melanotransferrin is not involved in iron metabolism, but plays a vital role in melanoma cell proliferation and tumorigenesis.
Title: The melanoma tumor antigen, melanotransferrin (p97): a 25-year hallmark--from iron metabolism to tumorigenesis.

results suggest a Plg-mediated internalization mechanism for the clearance of sMTf via annexin II and LRP
Title: Plasminogen-dependent internalization of soluble melanotransferrin involves the low-density lipoprotein receptor-related protein and annexin II.

The following MELTF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MELTF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11206	NM_005929.6 Latest version!	Homo sapiens melanotransferrin (MELTF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu05337	NM_033316.4 Latest version!	Homo sapiens melanotransferrin (MELTF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$216.30 $309.00
OHu58951	XM_011512851.2 Latest version!	Homo sapiens melanotransferrin (MELTF), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu58949	XM_011512850.2 Latest version!	Homo sapiens melanotransferrin (MELTF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
OHu58950	XM_006713643.3 Latest version!	Homo sapiens melanotransferrin (MELTF), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
View Old Accession Versions >>
OHu11206	NM_005929.5	Homo sapiens melanotransferrin (MELTF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$517.30 $739.00
OHu05337	NM_033316.3	Homo sapiens melanotransferrin (MELTF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MELTF ( NM_005929.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005920.2 Homo sapiens melanotransferrin (MELTF), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11206
Clone ID Related Accession (Same CDS sequence)	NM_005929.5 , NM_005929.6
Accession Version	NM_005929.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	melanotransferrin isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068302.18, AA442552.1, AH002920.2 and BQ447289.1. On Nov 22, 2018 this sequence version replaced NM_005929.5. Transcript Variant: This variant (1) encodes the longer isoform (1), as compared to variant 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AH002920.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000296350.10/ ENSP00000296350.5 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGGA CCTGTTTGGA GACGACCACA ATAAGAACGG GTTCAAAATG 
TTCGACTCCT CCAACTATCA TGGCCAAGAC CTGCTTTTCA AGGATGCCAC CGTCCGGGCG 
GTGCCTGTCG GAGAGAAAAC CACCTACCGC GGCTGGCTGG GGCTGGACTA CGTGGCGGCG 
CTGGAAGGGA TGTCGTCTCA GCAGTGCTCG GGCGCAGCGG CCCCGGCGCC CGGGGCGCCC 
CTGCTCCCGC TGCTGCTGCC CGCCCTCGCC GCCCGCCTGC TCCCGCCCGC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005920.2
CDS	116..2332
Translation	MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQCSGAAAPAPGAPLLPLLLPALAARLLPPAL

Target ORF information:

RefSeq Version	NM_005929.6
Organism	Homo sapiens(human)
Definition	Homo sapiens melanotransferrin (MELTF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005929.6

ORF Insert Sequence:

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGGA CCTGTTTGGA GACGACCACA ATAAGAACGG GTTCAAAATG 
TTCGACTCCT CCAACTATCA TGGCCAAGAC CTGCTTTTCA AGGATGCCAC CGTCCGGGCG 
GTGCCTGTCG GAGAGAAAAC CACCTACCGC GGCTGGCTGG GGCTGGACTA CGTGGCGGCG 
CTGGAAGGGA TGTCGTCTCA GCAGTGCTCG GGCGCAGCGG CCCCGGCGCC CGGGGCGCCC 
CTGCTCCCGC TGCTGCTGCC CGCCCTCGCC GCCCGCCTGC TCCCGCCCGC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELTF ( NM_033316.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_201573.1 Homo sapiens melanotransferrin (MELTF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05337
Clone ID Related Accession (Same CDS sequence)	NM_033316.3 , NM_033316.4
Accession Version	NM_033316.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	melanotransferrin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AU099352.1 and BC071910.1. On Mar 28, 2007 this sequence version replaced NM_033316.2. Transcript Variant: This variant (2) differs from variant 1 in the central coding region, resulting in a frameshift. The encoded isoform (2) has a truncated and distinct C-terminus and lacks the propeptide compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.414674.1, SRR1163658.168662.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGAAAGTCCA 
TCACGAAGGC AAACATGGAC CAGATCTGAG GAGGAAGAAG GCGAGTGCCC TGCACACGAG 
GAAGCACGTA GGACGATGCG CTCTAGTGCT GGGCAAGCCT GGAAATGGGC TCCCGTTCAC 
AGGCCCCAGG ACGAGTCTGA CAAAGGAGAA TTTGGAAAAC GGGCAAAGAG TAGGGATATG 
TTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_201573.1
CDS	114..1022
Translation	MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDESPSRRQTWTRSEEEEGECPAHEEARRTMRSSAGQAWKWAPVHRPQDESDKGEFGKRAKSRDMLG

Target ORF information:

RefSeq Version	NM_033316.3
Organism	Homo sapiens(human)
Definition	Homo sapiens melanotransferrin (MELTF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033316.3

ORF Insert Sequence:

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGAAAGTCCA 
TCACGAAGGC AAACATGGAC CAGATCTGAG GAGGAAGAAG GCGAGTGCCC TGCACACGAG 
GAAGCACGTA GGACGATGCG CTCTAGTGCT GGGCAAGCCT GGAAATGGGC TCCCGTTCAC 
AGGCCCCAGG ACGAGTCTGA CAAAGGAGAA TTTGGAAAAC GGGCAAAGAG TAGGGATATG 
TTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELTF ( NM_033316.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_201573.1 Homo sapiens melanotransferrin (MELTF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu05337
Clone ID Related Accession (Same CDS sequence)	NM_033316.3 , NM_033316.4
Accession Version	NM_033316.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	melanotransferrin isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC068302.18, AU099352.1 and BC071910.1. On May 31, 2019 this sequence version replaced NM_033316.3. Transcript Variant: This variant (2) differs from variant 1 in the central coding region, resulting in a frameshift. The encoded isoform (2) has a truncated and distinct C-terminus and lacks the propeptide compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC002623.2, SRR1163655.414674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGAAAGTCCA 
TCACGAAGGC AAACATGGAC CAGATCTGAG GAGGAAGAAG GCGAGTGCCC TGCACACGAG 
GAAGCACGTA GGACGATGCG CTCTAGTGCT GGGCAAGCCT GGAAATGGGC TCCCGTTCAC 
AGGCCCCAGG ACGAGTCTGA CAAAGGAGAA TTTGGAAAAC GGGCAAAGAG TAGGGATATG 
TTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_201573.1
CDS	116..1024
Translation	MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDESPSRRQTWTRSEEEEGECPAHEEARRTMRSSAGQAWKWAPVHRPQDESDKGEFGKRAKSRDMLG

Target ORF information:

RefSeq Version	NM_033316.4
Organism	Homo sapiens(human)
Definition	Homo sapiens melanotransferrin (MELTF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_033316.4

ORF Insert Sequence:

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGAAAGTCCA 
TCACGAAGGC AAACATGGAC CAGATCTGAG GAGGAAGAAG GCGAGTGCCC TGCACACGAG 
GAAGCACGTA GGACGATGCG CTCTAGTGCT GGGCAAGCCT GGAAATGGGC TCCCGTTCAC 
AGGCCCCAGG ACGAGTCTGA CAAAGGAGAA TTTGGAAAAC GGGCAAAGAG TAGGGATATG 
TTGGGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELTF ( XM_011512851.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511153.1 Homo sapiens melanotransferrin (MELTF), transcript variant X3, mRNA.

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CloneID	OHu58951
Clone ID Related Accession (Same CDS sequence)	XM_011512851.2
Accession Version	XM_011512851.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	melanotransferrin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512851.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGAA CCCCTCTATC CCAGGAGTGC TTTACTCTGG GTCCCTGGGC 
TTCCCTAGGG GGTTGCTAAG TCCTCTGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511153.1
CDS	114..2126
Translation	MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQNPSIPGVLYSGSLGFPRGLLSPLK

Target ORF information:

RefSeq Version	XM_011512851.2
Organism	Homo sapiens(human)
Definition	Homo sapiens melanotransferrin (MELTF), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512851.2

ORF Insert Sequence:

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGAA CCCCTCTATC CCAGGAGTGC TTTACTCTGG GTCCCTGGGC 
TTCCCTAGGG GGTTGCTAAG TCCTCTGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELTF ( NM_005929.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005920.2 Homo sapiens melanotransferrin (MELTF), transcript variant 1, mRNA.

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CloneID	OHu11206
Clone ID Related Accession (Same CDS sequence)	NM_005929.5 , NM_005929.6
Accession Version	NM_005929.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2217bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	melanotransferrin isoform 1 preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AA442552.1, AH002920.2, AC068302.18 and BQ447289.1. On Aug 10, 2010 this sequence version replaced NM_005929.4. Transcript Variant: This variant (1) encodes the longer isoform (1), as compared to variant 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGGA CCTGTTTGGA GACGACCACA ATAAGAACGG GTTCAAAATG 
TTCGACTCCT CCAACTATCA TGGCCAAGAC CTGCTTTTCA AGGATGCCAC CGTCCGGGCG 
GTGCCTGTCG GAGAGAAAAC CACCTACCGC GGCTGGCTGG GGCTGGACTA CGTGGCGGCG 
CTGGAAGGGA TGTCGTCTCA GCAGTGCTCG GGCGCAGCGG CCCCGGCGCC CGGGGCGCCC 
CTGCTCCCGC TGCTGCTGCC CGCCCTCGCC GCCCGCCTGC TCCCGCCCGC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005920.2
CDS	115..2331
Translation	MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQCSGAAAPAPGAPLLPLLLPALAARLLPPAL

Target ORF information:

RefSeq Version	NM_005929.5
Organism	Homo sapiens(human)
Definition	Homo sapiens melanotransferrin (MELTF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005929.5

ORF Insert Sequence:

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGGA CCTGTTTGGA GACGACCACA ATAAGAACGG GTTCAAAATG 
TTCGACTCCT CCAACTATCA TGGCCAAGAC CTGCTTTTCA AGGATGCCAC CGTCCGGGCG 
GTGCCTGTCG GAGAGAAAAC CACCTACCGC GGCTGGCTGG GGCTGGACTA CGTGGCGGCG 
CTGGAAGGGA TGTCGTCTCA GCAGTGCTCG GGCGCAGCGG CCCCGGCGCC CGGGGCGCCC 
CTGCTCCCGC TGCTGCTGCC CGCCCTCGCC GCCCGCCTGC TCCCGCCCGC CCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELTF ( XM_011512850.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511152.1 Homo sapiens melanotransferrin (MELTF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu58949
Clone ID Related Accession (Same CDS sequence)	XM_011512850.2
Accession Version	XM_011512850.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2298bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	melanotransferrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011512850.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGCA ATTTGCAGCT AGCGGCACTG GGCGGGTGAC CCTGCCTGAG 
CCCAGTGAGG AGCAGGTCGT CTGCCTGCGG TGCTTCCAGG ACCTGTTTGG AGACGACCAC 
AATAAGAACG GGTTCAAAAT GTTCGACTCC TCCAACTATC ATGGCCAAGA CCTGCTTTTC 
AAGGATGCCA CCGTCCGGGC GGTGCCTGTC GGAGAGAAAA CCACCTACCG CGGCTGGCTG 
GGGCTGGACT ACGTGGCGGC GCTGGAAGGG ATGTCGTCTC AGCAGTGCTC GGGCGCAGCG 
GCCCCGGCGC CCGGGGCGCC CCTGCTCCCG CTGCTGCTGC CCGCCCTCGC CGCCCGCCTG 
CTCCCGCCCG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511152.1
CDS	114..2411
Translation	MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTAVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQQFAASGTGRVTLPEPSEEQVVCLRCFQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQCSGAAAPAPGAPLLPLLLPALAARLLPPAL

Target ORF information:

RefSeq Version	XM_011512850.2
Organism	Homo sapiens(human)
Definition	Homo sapiens melanotransferrin (MELTF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011512850.2

ORF Insert Sequence:

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGCAGTG AGCGAGTTCT TCAATGCCAG CTGCGTGCCC 
GTGAACAACC CCAAGAACTA CCCCTCCTCG CTGTGTGCAC TGTGCGTGGG GGACGAGCAG 
GGCCGCAACA AGTGTGTGGG CAACAGCCAG GAGCGGTATT ACGGCTACCG CGGCGCCTTC 
AGGTGCCTGG TGGAGAATGC GGGTGACGTT GCCTTCGTCA GGCACACAAC CGTCTTTGAC 
AACACAAACG GCCACAATTC CGAGCCCTGG GCTGCTGAGC TCAGGTCAGA GGACTATGAA 
CTGCTGTGCC CCAACGGGGC CCGAGCCGAG GTGTCCCAGT TTGCAGCCTG CAACCTGGCA 
CAGATACCAC CCCACGCCGT GATGGTCCGG CCCGACACCA ACATCTTCAC CGTGTATGGA 
CTGCTGGACA AGGCCCAGCA ATTTGCAGCT AGCGGCACTG GGCGGGTGAC CCTGCCTGAG 
CCCAGTGAGG AGCAGGTCGT CTGCCTGCGG TGCTTCCAGG ACCTGTTTGG AGACGACCAC 
AATAAGAACG GGTTCAAAAT GTTCGACTCC TCCAACTATC ATGGCCAAGA CCTGCTTTTC 
AAGGATGCCA CCGTCCGGGC GGTGCCTGTC GGAGAGAAAA CCACCTACCG CGGCTGGCTG 
GGGCTGGACT ACGTGGCGGC GCTGGAAGGG ATGTCGTCTC AGCAGTGCTC GGGCGCAGCG 
GCCCCGGCGC CCGGGGCGCC CCTGCTCCCG CTGCTGCTGC CCGCCCTCGC CGCCCGCCTG 
CTCCCGCCCG CCCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MELTF ( XM_006713643.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006713706.2 Homo sapiens melanotransferrin (MELTF), transcript variant X2, mRNA.

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CloneID	OHu58950
Clone ID Related Accession (Same CDS sequence)	XM_006713643.3
Accession Version	XM_006713643.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	melanotransferrin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006713643.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGTGAGC GAGTTCTTCA ATGCCAGCTG CGTGCCCGTG 
AACAACCCCA AGAACTACCC CTCCTCGCTG TGTGCACTGT GCGTGGGGGA CGAGCAGGGC 
CGCAACAAGT GTGTGGGCAA CAGCCAGGAG CGGTATTACG GCTACCGCGG CGCCTTCAGG 
TGCCTGGTGG AGAATGCGGG TGACGTTGCC TTCGTCAGGC ACACAACCGT CTTTGACAAC 
ACAAACGGCC ACAATTCCGA GCCCTGGGCT GCTGAGCTCA GGTCAGAGGA CTATGAACTG 
CTGTGCCCCA ACGGGGCCCG AGCCGAGGTG TCCCAGTTTG CAGCCTGCAA CCTGGCACAG 
ATACCACCCC ACGCCGTGAT GGTCCGGCCC GACACCAACA TCTTCACCGT GTATGGACTG 
CTGGACAAGG CCCAGCAATT TGCAGCTAGC GGCACTGGGC GGGTGACCCT GCCTGAGCCC 
AGTGAGGAGC AGGTCGTCTG CCTGCGGTGC TTCCAGGACC TGTTTGGAGA CGACCACAAT 
AAGAACGGGT TCAAAATGTT CGACTCCTCC AACTATCATG GCCAAGACCT GCTTTTCAAG 
GATGCCACCG TCCGGGCGGT GCCTGTCGGA GAGAAAACCA CCTACCGCGG CTGGCTGGGG 
CTGGACTACG TGGCGGCGCT GGAAGGGATG TCGTCTCAGC AGTGCTCGGG CGCAGCGGCC 
CCGGCGCCCG GGGCGCCCCT GCTCCCGCTG CTGCTGCCCG CCCTCGCCGC CCGCCTGCTC 
CCGCCCGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006713706.2
CDS	114..2408
Translation	MRGPSGALWLLLALRTVLGGMEVRWCATSDPEQHKCGNMSEAFREAGIQPSLLCVRGTSADHCVQLIAAQEADAITLDGGAIYEAGKEHGLKPVVGEVYDQEVGTSYYAVAVVRRSSHVTIDTLKGVKSCHTGINRTVGWNVPVGYLVESGRLSVMGCDVLKAVSDYFGGSCVPGAGETSYSESLCRLCRGDSSGEGVCDKSPLERYYDYSGAFRCLAEGAGDVAFVKHSTVLENTDGKTLPSWGQALLSQDFELLCRDGSRADVTEWRQCHLARVPAHAVVVRADTDGGLIFRLLNEGQRLFSHEGSSFQMFSSEAYGQKDLLFKDSTSELVPIATQTYEAWLGHEYLHAMKGLLCDPNRLPPYLRWCVLSTPEIQKCGDMAVAFRRQRLKPEIQCVSAKSPQHCMERIQAEQVDAVTLSGEDIYTAGKTYGLVPAAGEHYAPEDSSNSYYVVAVVRRDSSHAFTLDELRGKRSCHAGFGSPAGWDVPVGALIQRGFIRPKDCDVLTVSEFFNASCVPVNNPKNYPSSLCALCVGDEQGRNKCVGNSQERYYGYRGAFRCLVENAGDVAFVRHTTVFDNTNGHNSEPWAAELRSEDYELLCPNGARAEVSQFAACNLAQIPPHAVMVRPDTNIFTVYGLLDKAQQFAASGTGRVTLPEPSEEQVVCLRCFQDLFGDDHNKNGFKMFDSSNYHGQDLLFKDATVRAVPVGEKTTYRGWLGLDYVAALEGMSSQQCSGAAAPAPGAPLLPLLLPALAARLLPPAL

Target ORF information:

RefSeq Version	XM_006713643.3
Organism	Homo sapiens(human)
Definition	Homo sapiens melanotransferrin (MELTF), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006713643.3

ORF Insert Sequence:

ATGCGGGGTC CGAGCGGGGC TCTGTGGCTG CTCCTGGCTC TGCGCACCGT GCTCGGTGGC 
ATGGAGGTGC GGTGGTGCGC CACCTCGGAC CCAGAGCAGC ACAAGTGCGG CAACATGAGC 
GAGGCCTTCC GGGAAGCGGG CATCCAGCCC TCCCTCCTCT GCGTCCGGGG CACCTCCGCC 
GACCACTGCG TCCAGCTCAT CGCGGCCCAG GAGGCTGACG CCATCACTCT GGATGGAGGA 
GCCATCTATG AGGCGGGAAA GGAGCACGGC CTGAAGCCGG TGGTGGGCGA AGTGTACGAT 
CAAGAGGTCG GTACCTCCTA TTACGCCGTG GCTGTGGTCA GGAGGAGCTC CCATGTGACC 
ATTGACACCC TGAAAGGCGT GAAGTCCTGC CACACGGGCA TCAATCGCAC AGTGGGCTGG 
AACGTGCCCG TGGGCTACCT GGTGGAGAGC GGCCGCCTCT CGGTGATGGG CTGCGATGTA 
CTCAAAGCTG TCAGCGACTA TTTTGGGGGC AGCTGCGTCC CGGGGGCAGG AGAGACCAGT 
TACTCTGAGT CCCTCTGTCG CCTCTGCAGG GGTGACAGCT CTGGGGAAGG GGTGTGTGAC 
AAGAGCCCCC TGGAGAGATA CTACGACTAC AGCGGGGCCT TCCGGTGCCT GGCGGAAGGG 
GCAGGGGACG TGGCTTTTGT GAAGCACAGC ACGGTACTGG AGAACACGGA TGGGAAGACG 
CTTCCCTCCT GGGGCCAGGC CCTGCTGTCA CAGGACTTCG AGCTGCTGTG CCGGGATGGT 
AGCCGGGCCG ATGTCACCGA GTGGAGGCAG TGCCATCTGG CCCGGGTGCC TGCTCACGCC 
GTGGTGGTCC GGGCCGACAC AGATGGGGGC CTCATCTTCC GGCTGCTCAA CGAAGGCCAG 
CGTCTGTTCA GCCACGAGGG CAGCAGCTTC CAGATGTTCA GCTCTGAGGC CTATGGCCAG 
AAGGATCTAC TCTTCAAAGA CTCTACCTCG GAGCTTGTGC CCATCGCCAC ACAGACCTAT 
GAGGCGTGGC TGGGCCATGA GTACCTGCAC GCCATGAAGG GTCTGCTCTG TGACCCCAAC 
CGGCTGCCCC CCTACCTGCG CTGGTGTGTG CTCTCCACTC CCGAGATCCA GAAGTGTGGA 
GACATGGCCG TGGCCTTCCG CCGGCAGCGG CTCAAGCCAG AGATCCAGTG CGTGTCAGCC 
AAGTCCCCCC AACACTGCAT GGAGCGGATC CAGGCTGAGC AGGTCGACGC TGTGACCCTG 
AGTGGCGAGG ACATTTACAC GGCGGGGAAG ACGTACGGCC TGGTTCCCGC AGCCGGGGAG 
CACTATGCCC CGGAAGACAG CAGCAACTCG TACTACGTGG TGGCCGTGGT GAGACGGGAC 
AGCTCCCACG CCTTCACCTT GGATGAGCTT CGGGGCAAGC GCTCCTGCCA CGCCGGTTTC 
GGCAGCCCTG CAGGCTGGGA TGTCCCCGTG GGTGCCCTTA TTCAGAGAGG CTTCATCCGG 
CCCAAGGACT GTGACGTCCT CACAGTGAGC GAGTTCTTCA ATGCCAGCTG CGTGCCCGTG 
AACAACCCCA AGAACTACCC CTCCTCGCTG TGTGCACTGT GCGTGGGGGA CGAGCAGGGC 
CGCAACAAGT GTGTGGGCAA CAGCCAGGAG CGGTATTACG GCTACCGCGG CGCCTTCAGG 
TGCCTGGTGG AGAATGCGGG TGACGTTGCC TTCGTCAGGC ACACAACCGT CTTTGACAAC 
ACAAACGGCC ACAATTCCGA GCCCTGGGCT GCTGAGCTCA GGTCAGAGGA CTATGAACTG 
CTGTGCCCCA ACGGGGCCCG AGCCGAGGTG TCCCAGTTTG CAGCCTGCAA CCTGGCACAG 
ATACCACCCC ACGCCGTGAT GGTCCGGCCC GACACCAACA TCTTCACCGT GTATGGACTG 
CTGGACAAGG CCCAGCAATT TGCAGCTAGC GGCACTGGGC GGGTGACCCT GCCTGAGCCC 
AGTGAGGAGC AGGTCGTCTG CCTGCGGTGC TTCCAGGACC TGTTTGGAGA CGACCACAAT 
AAGAACGGGT TCAAAATGTT CGACTCCTCC AACTATCATG GCCAAGACCT GCTTTTCAAG 
GATGCCACCG TCCGGGCGGT GCCTGTCGGA GAGAAAACCA CCTACCGCGG CTGGCTGGGG 
CTGGACTACG TGGCGGCGCT GGAAGGGATG TCGTCTCAGC AGTGCTCGGG CGCAGCGGCC 
CCGGCGCCCG GGGCGCCCCT GCTCCCGCTG CTGCTGCCCG CCCTCGCCGC CCGCCTGCTC 
CCGCCCGCCC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Discovery of melanotransferrin as a serological marker of colorectal cancer by secretome analysis and quantitative proteomics.
Journal of proteome research13(11)4919-31(2014 Nov)
Shin J,Kim HJ,Kim G,Song M,Woo SJ,Lee ST,Kim H,Lee C

PC3 prostate tumor-initiating cells with molecular profile FAM65Bhigh/MFI2low/LEF1low increase tumor angiogenesis.
Molecular cancer9319(2010 Dec)
Zhang K,Waxman DJ

MHC class II-associated proteins in B-cell exosomes and potential functional implications for exosome biogenesis.
Immunology and cell biology88(8)851-6(2010 Nov-Dec)
Buschow SI,van Balkom BW,Aalberts M,Heck AJ,Wauben M,Stoorvogel W

Meckel-Gruber syndrome protein MKS3 is required for endoplasmic reticulum-associated degradation of surfactant protein C.
The Journal of biological chemistry284(48)33377-83(2009 Nov)
Wang M,Bridges JP,Na CL,Xu Y,Weaver TE

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MELTF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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