MAP3K5 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017010872.1	XP_016866361.1	mitogen-activated protein kinase kinase kinase 5 isoform X3
XM_017010874.1	XP_016866363.1	mitogen-activated protein kinase kinase kinase 5 isoform X5
XM_011535839.3	XP_011534141.2	mitogen-activated protein kinase kinase kinase 5 isoform X7
XM_017010876.1	XP_016866365.1	mitogen-activated protein kinase kinase kinase 5 isoform X8
NM_005923.3	NP_005914.1	mitogen-activated protein kinase kinase kinase 5
XM_011535839.2	XP_011534141.2	mitogen-activated protein kinase kinase kinase 5 isoform X7
XM_017010877.1	XP_016866366.1	mitogen-activated protein kinase kinase kinase 5 isoform X9
NM_005923.4	NP_005914.1	mitogen-activated protein kinase kinase kinase 5
XM_017010870.1	XP_016866359.1	mitogen-activated protein kinase kinase kinase 5 isoform X1
XM_017010873.1	XP_016866362.1	mitogen-activated protein kinase kinase kinase 5 isoform X4
XM_017010875.1	XP_016866364.1	mitogen-activated protein kinase kinase kinase 5 isoform X6
XM_017010871.1	XP_016866360.1	mitogen-activated protein kinase kinase kinase 5 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19594	NM_005923.4 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu96613	XM_017010876.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu96614	XM_017010877.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu96606	XM_017010870.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96607	XM_017010871.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96608	XM_017010872.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96609	XM_017010873.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96610	XM_017010874.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96611	XM_017010875.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96612	XM_011535839.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
View Old Accession Versions >>
OHu96612	XM_011535839.2	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu19594	NM_005923.3	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
Hide Old Accession Versions >>

MAP3K5 ( NM_005923.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005914.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

Price & Availability↑

CloneID	OHu19594
Clone ID Related Accession (Same CDS sequence)	NM_005923.3 , NM_005923.4
Accession Version	NM_005923.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U67156.1. This sequence is a reference standard in the RefSeqGene project. On Jun 21, 2002 this sequence version replaced NM_005923.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U67156.1, SRR1803612.276620.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG 
GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC 
GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG 
GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG 
AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC 
GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC 
ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC 
CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC 
ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC 
CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT 
AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG 
AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT 
TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC 
AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG 
CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC 
TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG 
CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT 
AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA 
AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG 
TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA 
AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG 
GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT 
AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT 
TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG 
CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA 
TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC 
TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA 
GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA 
GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT 
GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA 
TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA 
CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC 
ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT 
GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG 
AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG 
CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT 
CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA 
AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT 
GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT 
CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC 
TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT 
GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA 
GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA 
TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC 
CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA 
CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT 
TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA 
TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT 
GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA 
AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT 
CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA 
TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA 
GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG 
AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC 
ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC 
AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA 
GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG 
AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT 
GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA 
CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC 
TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA 
CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT 
CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA 
ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT 
CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT 
GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT 
CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC 
ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005914.1
CDS	362..4486
Translation	MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT

Target ORF information:

RefSeq Version	NM_005923.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005923.3

ORF Insert Sequence:

ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG 
GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC 
GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG 
GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG 
AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC 
GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC 
ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC 
CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC 
ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC 
CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT 
AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG 
AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT 
TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC 
AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG 
CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC 
TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG 
CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT 
AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA 
AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG 
TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA 
AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG 
GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT 
AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT 
TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG 
CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA 
TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC 
TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA 
GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA 
GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT 
GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA 
TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA 
CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC 
ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT 
GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG 
AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG 
CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT 
CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA 
AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT 
GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT 
CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC 
TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT 
GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA 
GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA 
TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC 
CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA 
CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT 
TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA 
TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT 
GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA 
AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT 
CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA 
TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA 
GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG 
AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC 
ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC 
AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA 
GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG 
AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT 
GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA 
CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC 
TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA 
CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT 
CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA 
ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT 
CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT 
GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT 
CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC 
ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( NM_005923.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005914.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

Price & Availability↑

CloneID	OHu19594
Clone ID Related Accession (Same CDS sequence)	NM_005923.3 , NM_005923.4
Accession Version	NM_005923.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4125bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-02
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121933.15 and AL024508.1. This sequence is a reference standard in the RefSeqGene project. On Nov 26, 2018 this sequence version replaced NM_005923.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.22198.1, U67156.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000359015.5/ ENSP00000351908.4 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG 
GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC 
GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG 
GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG 
AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC 
GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC 
ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC 
CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC 
ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC 
CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT 
AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG 
AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT 
TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC 
AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG 
CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC 
TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG 
CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT 
AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA 
AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG 
TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA 
AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG 
GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT 
AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT 
TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG 
CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA 
TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC 
TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA 
GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA 
GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT 
GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA 
TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA 
CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC 
ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT 
GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG 
AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG 
CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT 
CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA 
AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT 
GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT 
CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC 
TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT 
GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA 
GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA 
TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC 
CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA 
CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT 
TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA 
TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT 
GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA 
AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT 
CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA 
TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA 
GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG 
AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC 
ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC 
AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA 
GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG 
AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT 
GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA 
CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC 
TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA 
CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT 
CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA 
ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT 
CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT 
GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT 
CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC 
ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005914.1
CDS	321..4445
Translation	MSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT

Target ORF information:

RefSeq Version	NM_005923.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005923.4

ORF Insert Sequence:

ATGAGCACGG AGGCGGACGA GGGCATCACT TTCTCTGTGC CACCCTTCGC CCCCTCGGGC 
TTCTGCACCA TCCCCGAGGG CGGCATCTGC AGGAGGGGAG GAGCGGCGGC GGTGGGCGAG 
GGCGAGGAGC ACCAGCTGCC ACCGCCGCCG CCGGGCAGCT TCTGGAACGT GGAGAGCGCC 
GCTGCCCCTG GCATCGGTTG TCCGGCGGCC ACCTCCTCGA GCAGTGCCAC CCGAGGCCGG 
GGCAGCTCTG TTGGCGGGGG CAGCCGACGG ACCACGGTGG CATATGTGAT CAACGAAGCG 
AGCCAAGGGC AACTGGTGGT GGCCGAGAGC GAGGCCCTGC AGAGCTTGCG GGAGGCGTGC 
GAGACAGTGG GCGCCACCCT GGAAACCCTG CATTTTGGGA AACTCGACTT TGGAGAAACC 
ACCGTGCTGG ACCGCTTTTA CAATGCAGAT ATTGCGGTGG TGGAGATGAG CGATGCCTTC 
CGGCAGCCGT CCTTGTTTTA CCACCTTGGG GTGAGAGAAA GTTTCAGCAT GGCCAACAAC 
ATCATCCTCT ACTGTGATAC TAACTCGGAC TCTCTGCAGT CACTGAAGGA AATAATTTGC 
CAGAAGAATA CTATGTGCAC TGGGAACTAC ACCTTTGTTC CTTACATGAT AACTCCACAT 
AACAAAGTCT ACTGCTGTGA CAGCAGCTTC ATGAAGGGGT TGACAGAGCT CATGCAACCG 
AACTTCGAGC TGCTTCTTGG ACCCATCTGC TTACCTCTTG TGGATCGTTT TATTCAACTT 
TTGAAGGTGG CACAAGCAAG TTCTAGCCAG TACTTCCGGG AATCTATACT CAATGACATC 
AGGAAAGCTC GTAATTTATA CACTGGTAAA GAATTGGCAG CTGAGTTGGC AAGAATTCGG 
CAGCGAGTAG ATAATATCGA AGTCTTGACA GCAGATATTG TCATAAATCT GTTACTTTCC 
TACAGAGATA TCCAGGACTA TGATTCTATT GTGAAGCTGG TAGAGACTTT AGAAAAACTG 
CCAACCTTTG ATTTGGCCTC CCATCACCAT GTGAAGTTTC ATTATGCATT TGCACTGAAT 
AGGAGAAATC TCCCTGGTGA CAGAGCAAAA GCTCTTGATA TTATGATTCC CATGGTGCAA 
AGCGAAGGAC AAGTTGCTTC AGATATGTAT TGCCTAGTTG GTCGAATCTA CAAAGATATG 
TTTTTGGACT CTAATTTCAC GGACACTGAA AGCAGAGACC ATGGAGCTTC TTGGTTCAAA 
AAGGCATTTG AATCTGAGCC AACACTACAG TCAGGAATTA ATTATGCGGT CCTCCTCCTG 
GCAGCTGGAC ACCAGTTTGA ATCTTCCTTT GAGCTCCGGA AAGTTGGGGT GAAGCTAAGT 
AGTCTTCTTG GTAAAAAGGG AAACTTGGAA AAACTCCAGA GCTACTGGGA AGTTGGATTT 
TTTCTGGGGG CCAGCGTCCT AGCCAATGAC CACATGAGAG TCATTCAAGC ATCTGAAAAG 
CTTTTTAAAC TGAAGACACC AGCATGGTAC CTCAAGTCTA TTGTAGAGAC AATTTTAATA 
TATAAGCATT TTGTGAAACT GACCACAGAA CAGCCTGTGG CCAAGCAAGA ACTTGTGGAC 
TTTTGGATGG ATTTCCTGGT CGAGGCCACA AAGACAGATG TTACTGTGGT TAGGTTTCCA 
GTATTAATAT TAGAACCAAC CAAAATCTAT CAACCTTCTT ATTTGTCTAT CAACAATGAA 
GTTGAGGAAA AGACAATCTC TATTTGGCAC GTGCTTCCTG ATGACAAGAA AGGTATACAT 
GAGTGGAATT TTAGTGCCTC TTCTGTCAGG GGAGTGAGTA TTTCTAAATT TGAAGAAAGA 
TGCTGCTTTC TTTATGTGCT TCACAATTCT GATGATTTCC AAATCTATTT CTGTACAGAA 
CTTCATTGTA AAAAGTTTTT TGAGATGGTG AACACCATTA CCGAAGAGAA GGGGAGAAGC 
ACAGAGGAAG GAGACTGTGA AAGTGACTTG CTGGAGTATG ACTATGAATA TGATGAAAAT 
GGTGACAGAG TCGTTTTAGG AAAAGGCACT TATGGGATAG TCTACGCAGG TCGGGACTTG 
AGCAACCAAG TCAGAATTGC TATTAAGGAA ATCCCAGAGA GAGACAGCAG ATACTCTCAG 
CCCCTGCATG AAGAAATAGC ATTGCATAAA CACCTGAAGC ACAAAAATAT TGTCCAGTAT 
CTGGGCTCTT TCAGTGAGAA TGGTTTCATT AAAATCTTCA TGGAGCAGGT CCCTGGAGGA 
AGTCTTTCTG CTCTCCTTCG TTCCAAATGG GGTCCATTAA AAGACAATGA GCAAACAATT 
GGCTTTTATA CAAAGCAAAT ACTGGAAGGA TTAAAATATC TCCATGACAA TCAGATAGTT 
CACCGGGACA TAAAGGGTGA CAATGTGTTG ATTAATACCT ACAGTGGTGT TCTCAAGATC 
TCTGACTTCG GAACATCAAA GAGGCTTGCT GGCATAAACC CCTGTACTGA AACTTTTACT 
GGTACCCTCC AGTATATGGC ACCAGAAATA ATAGATAAAG GACCAAGAGG CTACGGAAAA 
GCAGCAGACA TCTGGTCTCT GGGCTGTACA ATCATTGAAA TGGCCACAGG AAAACCCCCA 
TTTTATGAAC TGGGAGAACC ACAAGCAGCT ATGTTCAAGG TGGGAATGTT TAAAGTCCAC 
CCTGAGATCC CAGAGTCCAT GTCTGCAGAG GCCAAGGCAT TCATACTGAA ATGTTTTGAA 
CCAGATCCTG ACAAGAGAGC CTGTGCTAAC GACTTGCTTG TTGATGAGTT TTTAAAAGTT 
TCAAGCAAAA AGAAAAAGAC ACAACCTAAG CTTTCAGCTC TTTCAGCTGG ATCAAATGAA 
TATCTCAGGA GTATATCCTT GCCGGTACCT GTGCTGGTGG AGGACACCAG CAGCAGCAGT 
GAGTACGGCT CAGTTTCACC CGACACGGAG TTGAAAGTGG ACCCCTTCTC TTTCAAAACA 
AGAGCCAAGT CCTGCGGAGA AAGAGATGTC AAGGGAATTC GGACACTCTT TTTGGGCATT 
CCAGATGAGA ATTTTGAAGA TCACAGTGCT CCTCCTTCCC CTGAAGAAAA AGATTCTGGA 
TTCTTCATGC TGAGGAAGGA CAGTGAGAGG CGAGCTACCC TTCACAGGAT CCTGACGGAA 
GACCAAGACA AAATTGTGAG AAACCTAATG GAATCTTTAG CTCAGGGGGC TGAAGAACCG 
AAACTAAAAT GGGAACACAT CACAACCCTC ATTGCAAGCC TCAGAGAATT TGTGAGATCC 
ACTGACCGAA AAATCATAGC CACCACACTG TCAAAGCTGA AACTGGAGCT GGACTTCGAC 
AGCCATGGCA TTAGCCAAGT CCAGGTGGTA CTCTTTGGTT TTCAAGATGC TGTCAATAAA 
GTTCTTCGGA ATCATAACAT CAAGCCGCAC TGGATGTTTG CCTTAGACAG TATCATTCGG 
AAGGCGGTAC AGACAGCCAT TACCATCCTG GTTCCAGAAC TAAGGCCACA TTTCAGCCTT 
GCATCTGAGA GTGATACTGC TGATCAAGAA GACTTGGATG TAGAAGATGA CCATGAGGAA 
CAGCCTTCAA ATCAAACTGT CCGAAGACCT CAGGCTGTCA TTGAAGATGC TGTGGCTACC 
TCAGGCGTGA GCACGCTCAG TTCTACTGTG TCTCATGATT CCCAGAGTGC TCACCGGTCA 
CTGAATGTAC AGCTTGGAAG GATGAAAATA GAAACCAATA GATTACTGGA AGAATTGGTT 
CGGAAAGAGA AAGAATTACA AGCACTCCTT CATCGAGCTA TTGAAGAAAA AGACCAAGAA 
ATTAAACACC TGAAGCTTAA GTCCCAACCC ATAGAAATTC CTGAATTGCC TGTATTTCAT 
CTAAATTCTT CTGGCACAAA TACTGAAGAT TCTGAACTTA CCGACTGGCT GAGAGTGAAT 
GGAGCTGATG AAGACACTAT AAGCCGGTTT TTGGCTGAAG ATTATACACT ATTGGATGTT 
CTCTACTATG TTACACGTGA TGACTTAAAA TGCTTGAGAC TAAGGGGAGG GATGCTGTGC 
ACACTGTGGA AGGCTATCAT TGACTTTCGA AACAAACAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010876.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866365.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X8, mRNA.

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CloneID	OHu96613
Clone ID Related Accession (Same CDS sequence)	XM_017010876.1
Accession Version	XM_017010876.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG 
ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG 
GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA 
TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT 
GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC 
ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA 
GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT 
TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT 
ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT 
CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT 
GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT 
TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA 
GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC 
TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC 
ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT 
GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC 
AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT 
ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT 
TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT 
GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT 
TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA 
ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC 
GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC 
TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC 
TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA 
GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC 
AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG 
GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA 
GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC 
CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC 
AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC 
TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA 
CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG 
GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG 
GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC 
ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT 
GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT 
TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG 
GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG 
GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT 
CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC 
CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC 
CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA 
GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC 
CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG 
AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT 
TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT 
GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA 
CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT 
GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC 
ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT 
TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT 
AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT 
ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT 
CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT 
ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA 
GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA 
CTAAGGTACC ACTTTTCTTT CTGTTGTCAC TATGGAAAGA GCACCAGGCA AATATCAAAA 
AGTCTGAGTA TCTTCCCAGG CTGGGTCTCT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866365.1
CDS	275..3850
Translation	MQEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNAEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRYHFSFCCHYGKSTRQISKSLSIFPGWVSN

Target ORF information:

RefSeq Version	XM_017010876.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010876.1

ORF Insert Sequence:

ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG 
ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG 
GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA 
TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT 
GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC 
ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA 
GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT 
TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT 
ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT 
CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT 
GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT 
TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA 
GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC 
TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC 
ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT 
GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC 
AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT 
ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT 
TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT 
GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT 
TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA 
ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC 
GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC 
TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC 
TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA 
GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC 
AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG 
GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA 
GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC 
CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC 
AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC 
TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA 
CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG 
GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG 
GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC 
ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT 
GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT 
TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG 
GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG 
GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT 
CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC 
CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC 
CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA 
GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC 
CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG 
AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT 
TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT 
GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA 
CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT 
GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC 
ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT 
TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT 
AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT 
ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT 
CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT 
ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA 
GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA 
CTAAGGTACC ACTTTTCTTT CTGTTGTCAC TATGGAAAGA GCACCAGGCA AATATCAAAA 
AGTCTGAGTA TCTTCCCAGG CTGGGTCTCT AATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010877.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866366.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu96614
Clone ID Related Accession (Same CDS sequence)	XM_017010877.1
Accession Version	XM_017010877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3546bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG 
ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG 
GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA 
TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT 
GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC 
ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA 
GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT 
TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT 
ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT 
CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT 
GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT 
TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA 
GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC 
TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC 
ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT 
GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC 
AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT 
ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT 
TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT 
GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT 
TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA 
ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC 
GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC 
TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC 
TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA 
GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC 
AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG 
GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA 
GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC 
CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC 
AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC 
TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA 
CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG 
GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG 
GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC 
ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT 
GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT 
TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG 
GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG 
GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT 
CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC 
CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC 
CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA 
GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC 
CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG 
AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT 
TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT 
GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA 
CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT 
GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC 
ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT 
TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT 
AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT 
ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT 
CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT 
ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA 
GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA 
CTAAGGGGAG GGATGCTGTG CACACTGTGG AAGGCTATCA TTGACTTTCG AAACAAACAG 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866366.1
CDS	512..4057
Translation	MQEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNAEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT

Target ORF information:

RefSeq Version	XM_017010877.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010877.1

ORF Insert Sequence:

ATGCAGGAAA TAATTTGCCA GAAGAATACT ATGTGCACTG GGAACTACAC CTTTGTTCCT 
TACATGATAA CTCCACATAA CAAAGTCTAC TGCTGTGACA GCAGCTTCAT GAAGGGGTTG 
ACAGAGCTCA TGCAACCGAA CTTCGAGCTG CTTCTTGGAC CCATCTGCTT ACCTCTTGTG 
GATCGTTTTA TTCAACTTTT GAAGGTGGCA CAAGCAAGTT CTAGCCAGTA CTTCCGGGAA 
TCTATACTCA ATGACATCAG GAAAGCTCGT AATTTATACA CTGGTAAAGA ATTGGCAGCT 
GAGTTGGCAA GAATTCGGCA GCGAGTAGAT AATATCGAAG TCTTGACAGC AGATATTGTC 
ATAAATCTGT TACTTTCCTA CAGAGATATC CAGGACTATG ATTCTATTGT GAAGCTGGTA 
GAGACTTTAG AAAAACTGCC AACCTTTGAT TTGGCCTCCC ATCACCATGT GAAGTTTCAT 
TATGCATTTG CACTGAATAG GAGAAATCTC CCTGGTGACA GAGCAAAAGC TCTTGATATT 
ATGATTCCCA TGGTGCAAAG CGAAGGACAA GTTGCTTCAG ATATGTATTG CCTAGTTGGT 
CGAATCTACA AAGATATGTT TTTGGACTCT AATTTCACGG ACACTGAAAG CAGAGACCAT 
GGAGCTTCTT GGTTCAAAAA GGCATTTGAA TCTGAGCCAA CACTACAGTC AGGAATTAAT 
TATGCGGTCC TCCTCCTGGC AGCTGGACAC CAGTTTGAAT CTTCCTTTGA GCTCCGGAAA 
GTTGGGGTGA AGCTAAGTAG TCTTCTTGGT AAAAAGGGAA ACTTGGAAAA ACTCCAGAGC 
TACTGGGAAG TTGGATTTTT TCTGGGGGCC AGCGTCCTAG CCAATGACCA CATGAGAGTC 
ATTCAAGCAT CTGAAAAGCT TTTTAAACTG AAGACACCAG CATGGTACCT CAAGTCTATT 
GTAGAGACAA TTTTAATATA TAAGCATTTT GTGAAACTGA CCACAGAACA GCCTGTGGCC 
AAGCAAGAAC TTGTGGACTT TTGGATGGAT TTCCTGGTCG AGGCCACAAA GACAGATGTT 
ACTGTGGTTA GGTTTCCAGT ATTAATATTA GAACCAACCA AAATCTATCA ACCTTCTTAT 
TTGTCTATCA ACAATGAAGT TGAGGAAAAG ACAATCTCTA TTTGGCACGT GCTTCCTGAT 
GACAAGAAAG GTATACATGA GTGGAATTTT AGTGCCTCTT CTGTCAGGGG AGTGAGTATT 
TCTAAATTTG AAGAAAGATG CTGCTTTCTT TATGTGCTTC ACAATTCTGA TGATTTCCAA 
ATCTATTTCT GTACAGAACT TCATTGTAAA AAGTTTTTTG AGATGGTGAA CACCATTACC 
GAAGAGAAGG GGAGAAGCAC AGAGGAAGGA GACTGTGAAA GTGACTTGCT GGAGTATGAC 
TATGAATATG ATGAAAATGG TGACAGAGTC GTTTTAGGAA AAGGCACTTA TGGGATAGTC 
TACGCAGGTC GGGACTTGAG CAACCAAGTC AGAATTGCTA TTAAGGAAAT CCCAGAGAGA 
GACAGCAGAT ACTCTCAGCC CCTGCATGAA GAAATAGCAT TGCATAAACA CCTGAAGCAC 
AAAAATATTG TCCAGTATCT GGGCTCTTTC AGTGAGAATG GTTTCATTAA AATCTTCATG 
GAGCAGGTCC CTGGAGGAAG TCTTTCTGCT CTCCTTCGTT CCAAATGGGG TCCATTAAAA 
GACAATGAGC AAACAATTGG CTTTTATACA AAGCAAATAC TGGAAGGATT AAAATATCTC 
CATGACAATC AGATAGTTCA CCGGGACATA AAGGGTGACA ATGTGTTGAT TAATACCTAC 
AGTGGTGTTC TCAAGATCTC TGACTTCGGA ACATCAAAGA GGCTTGCTGG CATAAACCCC 
TGTACTGAAA CTTTTACTGG TACCCTCCAG TATATGGCAC CAGAAATAAT AGATAAAGGA 
CCAAGAGGCT ACGGAAAAGC AGCAGACATC TGGTCTCTGG GCTGTACAAT CATTGAAATG 
GCCACAGGAA AACCCCCATT TTATGAACTG GGAGAACCAC AAGCAGCTAT GTTCAAGGTG 
GGAATGTTTA AAGTCCACCC TGAGATCCCA GAGTCCATGT CTGCAGAGGC CAAGGCATTC 
ATACTGAAAT GTTTTGAACC AGATCCTGAC AAGAGAGCCT GTGCTAACGA CTTGCTTGTT 
GATGAGTTTT TAAAAGTTTC AAGCAAAAAG AAAAAGACAC AACCTAAGCT TTCAGCTCTT 
TCAGCTGGAT CAAATGCAGA ATATCTCAGG AGTATATCCT TGCCGGTACC TGTGCTGGTG 
GAGGACACCA GCAGCAGCAG TGAGTACGGC TCAGTTTCAC CCGACACGGA GTTGAAAGTG 
GACCCCTTCT CTTTCAAAAC AAGAGCCAAG TCCTGCGGAG AAAGAGATGT CAAGGGAATT 
CGGACACTCT TTTTGGGCAT TCCAGATGAG AATTTTGAAG ATCACAGTGC TCCTCCTTCC 
CCTGAAGAAA AAGATTCTGG ATTCTTCATG CTGAGGAAGG ACAGTGAGAG GCGAGCTACC 
CTTCACAGGA TCCTGACGGA AGACCAAGAC AAAATTGTGA GAAACCTAAT GGAATCTTTA 
GCTCAGGGGG CTGAAGAACC GAAACTAAAA TGGGAACACA TCACAACCCT CATTGCAAGC 
CTCAGAGAAT TTGTGAGATC CACTGACCGA AAAATCATAG CCACCACACT GTCAAAGCTG 
AAACTGGAGC TGGACTTCGA CAGCCATGGC ATTAGCCAAG TCCAGGTGGT ACTCTTTGGT 
TTTCAAGATG CTGTCAATAA AGTTCTTCGG AATCATAACA TCAAGCCGCA CTGGATGTTT 
GCCTTAGACA GTATCATTCG GAAGGCGGTA CAGACAGCCA TTACCATCCT GGTTCCAGAA 
CTAAGGCCAC ATTTCAGCCT TGCATCTGAG AGTGATACTG CTGATCAAGA AGACTTGGAT 
GTAGAAGATG ACCATGAGGA ACAGCCTTCA AATCAAACTG TCCGAAGACC TCAGGCTGTC 
ATTGAAGATG CTGTGGCTAC CTCAGGCGTG AGCACGCTCA GTTCTACTGT GTCTCATGAT 
TCCCAGAGTG CTCACCGGTC ACTGAATGTA CAGCTTGGAA GGATGAAAAT AGAAACCAAT 
AGATTACTGG AAGAATTGGT TCGGAAAGAG AAAGAATTAC AAGCACTCCT TCATCGAGCT 
ATTGAAGAAA AAGACCAAGA AATTAAACAC CTGAAGCTTA AGTCCCAACC CATAGAAATT 
CCTGAATTGC CTGTATTTCA TCTAAATTCT TCTGGCACAA ATACTGAAGA TTCTGAACTT 
ACCGACTGGC TGAGAGTGAA TGGAGCTGAT GAAGACACTA TAAGCCGGTT TTTGGCTGAA 
GATTATACAC TATTGGATGT TCTCTACTAT GTTACACGTG ATGACTTAAA ATGCTTGAGA 
CTAAGGGGAG GGATGCTGTG CACACTGTGG AAGGCTATCA TTGACTTTCG AAACAAACAG 
ACTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010870.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866359.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu96606
Clone ID Related Accession (Same CDS sequence)	XM_017010870.1
Accession Version	XM_017010870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4485bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA 
GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT 
CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG 
TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA 
GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT 
CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC 
ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT 
TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT 
TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA 
TGCTTGAGAC TAAGGTACCA CTTTTCTTTC TGTTGTCACT ATGGAAAGAG CACCAGGCAA 
ATATCAAAAA GTCTGAGTAT CTTCCCAGGC TGGGTCTCTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866359.1
CDS	358..4842
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNAEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRYHFSFCCHYGKSTRQISKSLSIFPGWVSN

Target ORF information:

RefSeq Version	XM_017010870.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010870.1

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA 
GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT 
CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG 
TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA 
GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT 
CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC 
ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT 
TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT 
TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA 
TGCTTGAGAC TAAGGTACCA CTTTTCTTTC TGTTGTCACT ATGGAAAGAG CACCAGGCAA 
ATATCAAAAA GTCTGAGTAT CTTCCCAGGC TGGGTCTCTA ATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010871.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866360.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA.

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CloneID	OHu96607
Clone ID Related Accession (Same CDS sequence)	XM_017010871.1
Accession Version	XM_017010871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG 
CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG 
AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG 
GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT 
CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA 
GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA 
TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT 
GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA 
AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC 
TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG 
ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT 
CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC 
TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG 
GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT 
CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA 
ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT 
CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA 
GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT 
GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG 
GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC 
TTGAGACTAA GGTACCACTT TTCTTTCTGT TGTCACTATG GAAAGAGCAC CAGGCAAATA 
TCAAAAAGTC TGAGTATCTT CCCAGGCTGG GTCTCTAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866360.1
CDS	358..4839
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRYHFSFCCHYGKSTRQISKSLSIFPGWVSN

Target ORF information:

RefSeq Version	XM_017010871.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010871.1

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG 
CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG 
AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG 
GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT 
CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA 
GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA 
TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT 
GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA 
AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC 
TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG 
ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT 
CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC 
TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG 
GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT 
CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA 
ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT 
CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA 
GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT 
GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG 
GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC 
TTGAGACTAA GGTACCACTT TTCTTTCTGT TGTCACTATG GAAAGAGCAC CAGGCAAATA 
TCAAAAAGTC TGAGTATCTT CCCAGGCTGG GTCTCTAATT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010872.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866361.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X3, mRNA.

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CloneID	OHu96608
Clone ID Related Accession (Same CDS sequence)	XM_017010872.1
Accession Version	XM_017010872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4455bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA 
GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT 
CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG 
TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA 
GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT 
CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC 
ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT 
TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT 
TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA 
TGCTTGAGAC TAAGGGGAGG GATGCTGTGC ACACTGTGGA AGGCTATCAT TGACTTTCGA 
AACAAACAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866361.1
CDS	357..4811
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNAEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT

Target ORF information:

RefSeq Version	XM_017010872.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010872.1

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGAAC TAAGGCCACA TTTCAGCCTT GCATCTGAGA GTGATACTGC TGATCAAGAA 
GACTTGGATG TAGAAGATGA CCATGAGGAA CAGCCTTCAA ATCAAACTGT CCGAAGACCT 
CAGGCTGTCA TTGAAGATGC TGTGGCTACC TCAGGCGTGA GCACGCTCAG TTCTACTGTG 
TCTCATGATT CCCAGAGTGC TCACCGGTCA CTGAATGTAC AGCTTGGAAG GATGAAAATA 
GAAACCAATA GATTACTGGA AGAATTGGTT CGGAAAGAGA AAGAATTACA AGCACTCCTT 
CATCGAGCTA TTGAAGAAAA AGACCAAGAA ATTAAACACC TGAAGCTTAA GTCCCAACCC 
ATAGAAATTC CTGAATTGCC TGTATTTCAT CTAAATTCTT CTGGCACAAA TACTGAAGAT 
TCTGAACTTA CCGACTGGCT GAGAGTGAAT GGAGCTGATG AAGACACTAT AAGCCGGTTT 
TTGGCTGAAG ATTATACACT ATTGGATGTT CTCTACTATG TTACACGTGA TGACTTAAAA 
TGCTTGAGAC TAAGGGGAGG GATGCTGTGC ACACTGTGGA AGGCTATCAT TGACTTTCGA 
AACAAACAGA CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010873.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866362.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu96609
Clone ID Related Accession (Same CDS sequence)	XM_017010873.1
Accession Version	XM_017010873.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4452bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG 
CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG 
AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG 
GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT 
CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA 
GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA 
TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT 
GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA 
AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC 
TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG 
ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT 
CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC 
TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG 
GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT 
CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA 
ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT 
CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA 
GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT 
GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG 
GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC 
TTGAGACTAA GGGGAGGGAT GCTGTGCACA CTGTGGAAGG CTATCATTGA CTTTCGAAAC 
AAACAGACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866362.1
CDS	356..4807
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT

Target ORF information:

RefSeq Version	XM_017010873.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010873.1

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGAATAT CTCAGGAGTA TATCCTTGCC GGTACCTGTG 
CTGGTGGAGG ACACCAGCAG CAGCAGTGAG TACGGCTCAG TTTCACCCGA CACGGAGTTG 
AAAGTGGACC CCTTCTCTTT CAAAACAAGA GCCAAGTCCT GCGGAGAAAG AGATGTCAAG 
GGAATTCGGA CACTCTTTTT GGGCATTCCA GATGAGAATT TTGAAGATCA CAGTGCTCCT 
CCTTCCCCTG AAGAAAAAGA TTCTGGATTC TTCATGCTGA GGAAGGACAG TGAGAGGCGA 
GCTACCCTTC ACAGGATCCT GACGGAAGAC CAAGACAAAA TTGTGAGAAA CCTAATGGAA 
TCTTTAGCTC AGGGGGCTGA AGAACCGAAA CTAAAATGGG AACACATCAC AACCCTCATT 
GCAAGCCTCA GAGAATTTGT GAGATCCACT GACCGAAAAA TCATAGCCAC CACACTGTCA 
AAGCTGAAAC TGGAGCTGGA CTTCGACAGC CATGGCATTA GCCAAGTCCA GGTGGTACTC 
TTTGGTTTTC AAGATGCTGT CAATAAAGTT CTTCGGAATC ATAACATCAA GCCGCACTGG 
ATGTTTGCCT TAGACAGTAT CATTCGGAAG GCGGTACAGA CAGCCATTAC CATCCTGGTT 
CCAGAACTAA GGCCACATTT CAGCCTTGCA TCTGAGAGTG ATACTGCTGA TCAAGAAGAC 
TTGGATGTAG AAGATGACCA TGAGGAACAG CCTTCAAATC AAACTGTCCG AAGACCTCAG 
GCTGTCATTG AAGATGCTGT GGCTACCTCA GGCGTGAGCA CGCTCAGTTC TACTGTGTCT 
CATGATTCCC AGAGTGCTCA CCGGTCACTG AATGTACAGC TTGGAAGGAT GAAAATAGAA 
ACCAATAGAT TACTGGAAGA ATTGGTTCGG AAAGAGAAAG AATTACAAGC ACTCCTTCAT 
CGAGCTATTG AAGAAAAAGA CCAAGAAATT AAACACCTGA AGCTTAAGTC CCAACCCATA 
GAAATTCCTG AATTGCCTGT ATTTCATCTA AATTCTTCTG GCACAAATAC TGAAGATTCT 
GAACTTACCG ACTGGCTGAG AGTGAATGGA GCTGATGAAG ACACTATAAG CCGGTTTTTG 
GCTGAAGATT ATACACTATT GGATGTTCTC TACTATGTTA CACGTGATGA CTTAAAATGC 
TTGAGACTAA GGGGAGGGAT GCTGTGCACA CTGTGGAAGG CTATCATTGA CTTTCGAAAC 
AAACAGACTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010874.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866363.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu96610
Clone ID Related Accession (Same CDS sequence)	XM_017010874.1
Accession Version	XM_017010874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4377bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT 
TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT 
CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT 
GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA 
AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA 
AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT 
ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA 
TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT 
GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT 
TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA 
CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC 
AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG 
AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA 
CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG 
GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA 
CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG 
TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC 
TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA 
CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGCAG AATATCTCAG GAGTATATCC 
TTGCCGGTAC CTGTGCTGGT GGAGGACACC AGCAGCAGCA GTGAGTACGG CTCAGTTTCA 
CCCGACACGG AGTTGAAAGT GGACCCCTTC TCTTTCAAAA CAAGAGCCAA GTCCTGCGGA 
GAAAGAGATG TCAAGGGAAT TCGGACACTC TTTTTGGGCA TTCCAGATGA GAATTTTGAA 
GATCACAGTG CTCCTCCTTC CCCTGAAGAA AAAGATTCTG GATTCTTCAT GCTGAGGAAG 
GACAGTGAGA GGCGAGCTAC CCTTCACAGG ATCCTGACGG AAGACCAAGA CAAAATTGTG 
AGAAACCTAA TGGAATCTTT AGCTCAGGGG GCTGAAGAAC CGAAACTAAA ATGGGAACAC 
ATCACAACCC TCATTGCAAG CCTCAGAGAA TTTGTGAGAT CCACTGACCG AAAAATCATA 
GCCACCACAC TGTCAAAGCT GAAACTGGAG CTGGACTTCG ACAGCCATGG CATTAGCCAA 
GTCCAGGTGG TACTCTTTGG TTTTCAAGAT GCTGTCAATA AAGTTCTTCG GAATCATAAC 
ATCAAGCCGC ACTGGATGTT TGCCTTAGAC AGTATCATTC GGAAGGCGGT ACAGACAGCC 
ATTACCATCC TGGTTCCAGA ACTAAGGCCA CATTTCAGCC TTGCATCTGA GAGTGATACT 
GCTGATCAAG AAGACTTGGA TGTAGAAGAT GACCATGAGG AACAGCCTTC AAATCAAACT 
GTCCGAAGAC CTCAGGCTGT CATTGAAGAT GCTGTGGCTA CCTCAGGCGT GAGCACGCTC 
AGTTCTACTG TGTCTCATGA TTCCCAGAGT GCTCACCGGT CACTGAATGT ACAGCTTGGA 
AGGATGAAAA TAGAAACCAA TAGATTACTG GAAGAATTGG TTCGGAAAGA GAAAGAATTA 
CAAGCACTCC TTCATCGAGC TATTGAAGAA AAAGACCAAG AAATTAAACA CCTGAAGCTT 
AAGTCCCAAC CCATAGAAAT TCCTGAATTG CCTGTATTTC ATCTAAATTC TTCTGGCACA 
AATACTGAAG ATTCTGAACT TACCGACTGG CTGAGAGTGA ATGGAGCTGA TGAAGACACT 
ATAAGCCGGT TTTTGGCTGA AGATTATACA CTATTGGATG TTCTCTACTA TGTTACACGT 
GATGACTTAA AATGCTTGAG ACTAAGGTAC CACTTTTCTT TCTGTTGTCA CTATGGAAAG 
AGCACCAGGC AAATATCAAA AAGTCTGAGT ATCTTCCCAG GCTGGGTCTC TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866363.1
CDS	358..4734
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNAEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRYHFSFCCHYGKSTRQISKSLSIFPGWVSN

Target ORF information:

RefSeq Version	XM_017010874.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010874.1

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT 
TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT 
CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT 
GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA 
AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA 
AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT 
ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA 
TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT 
GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT 
TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA 
CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC 
AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG 
AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA 
CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG 
GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA 
CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG 
TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC 
TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA 
CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGCAG AATATCTCAG GAGTATATCC 
TTGCCGGTAC CTGTGCTGGT GGAGGACACC AGCAGCAGCA GTGAGTACGG CTCAGTTTCA 
CCCGACACGG AGTTGAAAGT GGACCCCTTC TCTTTCAAAA CAAGAGCCAA GTCCTGCGGA 
GAAAGAGATG TCAAGGGAAT TCGGACACTC TTTTTGGGCA TTCCAGATGA GAATTTTGAA 
GATCACAGTG CTCCTCCTTC CCCTGAAGAA AAAGATTCTG GATTCTTCAT GCTGAGGAAG 
GACAGTGAGA GGCGAGCTAC CCTTCACAGG ATCCTGACGG AAGACCAAGA CAAAATTGTG 
AGAAACCTAA TGGAATCTTT AGCTCAGGGG GCTGAAGAAC CGAAACTAAA ATGGGAACAC 
ATCACAACCC TCATTGCAAG CCTCAGAGAA TTTGTGAGAT CCACTGACCG AAAAATCATA 
GCCACCACAC TGTCAAAGCT GAAACTGGAG CTGGACTTCG ACAGCCATGG CATTAGCCAA 
GTCCAGGTGG TACTCTTTGG TTTTCAAGAT GCTGTCAATA AAGTTCTTCG GAATCATAAC 
ATCAAGCCGC ACTGGATGTT TGCCTTAGAC AGTATCATTC GGAAGGCGGT ACAGACAGCC 
ATTACCATCC TGGTTCCAGA ACTAAGGCCA CATTTCAGCC TTGCATCTGA GAGTGATACT 
GCTGATCAAG AAGACTTGGA TGTAGAAGAT GACCATGAGG AACAGCCTTC AAATCAAACT 
GTCCGAAGAC CTCAGGCTGT CATTGAAGAT GCTGTGGCTA CCTCAGGCGT GAGCACGCTC 
AGTTCTACTG TGTCTCATGA TTCCCAGAGT GCTCACCGGT CACTGAATGT ACAGCTTGGA 
AGGATGAAAA TAGAAACCAA TAGATTACTG GAAGAATTGG TTCGGAAAGA GAAAGAATTA 
CAAGCACTCC TTCATCGAGC TATTGAAGAA AAAGACCAAG AAATTAAACA CCTGAAGCTT 
AAGTCCCAAC CCATAGAAAT TCCTGAATTG CCTGTATTTC ATCTAAATTC TTCTGGCACA 
AATACTGAAG ATTCTGAACT TACCGACTGG CTGAGAGTGA ATGGAGCTGA TGAAGACACT 
ATAAGCCGGT TTTTGGCTGA AGATTATACA CTATTGGATG TTCTCTACTA TGTTACACGT 
GATGACTTAA AATGCTTGAG ACTAAGGTAC CACTTTTCTT TCTGTTGTCA CTATGGAAAG 
AGCACCAGGC AAATATCAAA AAGTCTGAGT ATCTTCCCAG GCTGGGTCTC TAATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_017010875.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016866364.1 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X6, mRNA.

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CloneID	OHu96611
Clone ID Related Accession (Same CDS sequence)	XM_017010875.1
Accession Version	XM_017010875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT 
TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT 
CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT 
GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA 
AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA 
AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT 
ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA 
TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT 
GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT 
TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA 
CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC 
AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG 
AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA 
CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG 
GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA 
CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG 
TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC 
TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA 
CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGAAT ATCTCAGGAG TATATCCTTG 
CCGGTACCTG TGCTGGTGGA GGACACCAGC AGCAGCAGTG AGTACGGCTC AGTTTCACCC 
GACACGGAGT TGAAAGTGGA CCCCTTCTCT TTCAAAACAA GAGCCAAGTC CTGCGGAGAA 
AGAGATGTCA AGGGAATTCG GACACTCTTT TTGGGCATTC CAGATGAGAA TTTTGAAGAT 
CACAGTGCTC CTCCTTCCCC TGAAGAAAAA GATTCTGGAT TCTTCATGCT GAGGAAGGAC 
AGTGAGAGGC GAGCTACCCT TCACAGGATC CTGACGGAAG ACCAAGACAA AATTGTGAGA 
AACCTAATGG AATCTTTAGC TCAGGGGGCT GAAGAACCGA AACTAAAATG GGAACACATC 
ACAACCCTCA TTGCAAGCCT CAGAGAATTT GTGAGATCCA CTGACCGAAA AATCATAGCC 
ACCACACTGT CAAAGCTGAA ACTGGAGCTG GACTTCGACA GCCATGGCAT TAGCCAAGTC 
CAGGTGGTAC TCTTTGGTTT TCAAGATGCT GTCAATAAAG TTCTTCGGAA TCATAACATC 
AAGCCGCACT GGATGTTTGC CTTAGACAGT ATCATTCGGA AGGCGGTACA GACAGCCATT 
ACCATCCTGG TTCCAGAACT AAGGCCACAT TTCAGCCTTG CATCTGAGAG TGATACTGCT 
GATCAAGAAG ACTTGGATGT AGAAGATGAC CATGAGGAAC AGCCTTCAAA TCAAACTGTC 
CGAAGACCTC AGGCTGTCAT TGAAGATGCT GTGGCTACCT CAGGCGTGAG CACGCTCAGT 
TCTACTGTGT CTCATGATTC CCAGAGTGCT CACCGGTCAC TGAATGTACA GCTTGGAAGG 
ATGAAAATAG AAACCAATAG ATTACTGGAA GAATTGGTTC GGAAAGAGAA AGAATTACAA 
GCACTCCTTC ATCGAGCTAT TGAAGAAAAA GACCAAGAAA TTAAACACCT GAAGCTTAAG 
TCCCAACCCA TAGAAATTCC TGAATTGCCT GTATTTCATC TAAATTCTTC TGGCACAAAT 
ACTGAAGATT CTGAACTTAC CGACTGGCTG AGAGTGAATG GAGCTGATGA AGACACTATA 
AGCCGGTTTT TGGCTGAAGA TTATACACTA TTGGATGTTC TCTACTATGT TACACGTGAT 
GACTTAAAAT GCTTGAGACT AAGGGGAGGG ATGCTGTGCA CACTGTGGAA GGCTATCATT 
GACTTTCGAA ACAAACAGAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016866364.1
CDS	357..4700
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPELRPHFSLASESDTADQEDLDVEDDHEEQPSNQTVRRPQAVIEDAVATSGVSTLSSTVSHDSQSAHRSLNVQLGRMKIETNRLLEELVRKEKELQALLHRAIEEKDQEIKHLKLKSQPIEIPELPVFHLNSSGTNTEDSELTDWLRVNGADEDTISRFLAEDYTLLDVLYYVTRDDLKCLRLRGGMLCTLWKAIIDFRNKQT

Target ORF information:

RefSeq Version	XM_017010875.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017010875.1

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAAAA GGTATACATG AGTGGAATTT TAGTGCCTCT 
TCTGTCAGGG GAGTGAGTAT TTCTAAATTT GAAGAAAGAT GCTGCTTTCT TTATGTGCTT 
CACAATTCTG ATGATTTCCA AATCTATTTC TGTACAGAAC TTCATTGTAA AAAGTTTTTT 
GAGATGGTGA ACACCATTAC CGAAGAGAAG GGGAGAAGCA CAGAGGAAGG AGACTGTGAA 
AGTGACTTGC TGGAGTATGA CTATGAATAT GATGAAAATG GTGACAGAGT CGTTTTAGGA 
AAAGGCACTT ATGGGATAGT CTACGCAGGT CGGGACTTGA GCAACCAAGT CAGAATTGCT 
ATTAAGGAAA TCCCAGAGAG AGACAGCAGA TACTCTCAGC CCCTGCATGA AGAAATAGCA 
TTGCATAAAC ACCTGAAGCA CAAAAATATT GTCCAGTATC TGGGCTCTTT CAGTGAGAAT 
GGTTTCATTA AAATCTTCAT GGAGCAGGTC CCTGGAGGAA GTCTTTCTGC TCTCCTTCGT 
TCCAAATGGG GTCCATTAAA AGACAATGAG CAAACAATTG GCTTTTATAC AAAGCAAATA 
CTGGAAGGAT TAAAATATCT CCATGACAAT CAGATAGTTC ACCGGGACAT AAAGGGTGAC 
AATGTGTTGA TTAATACCTA CAGTGGTGTT CTCAAGATCT CTGACTTCGG AACATCAAAG 
AGGCTTGCTG GCATAAACCC CTGTACTGAA ACTTTTACTG GTACCCTCCA GTATATGGCA 
CCAGAAATAA TAGATAAAGG ACCAAGAGGC TACGGAAAAG CAGCAGACAT CTGGTCTCTG 
GGCTGTACAA TCATTGAAAT GGCCACAGGA AAACCCCCAT TTTATGAACT GGGAGAACCA 
CAAGCAGCTA TGTTCAAGGT GGGAATGTTT AAAGTCCACC CTGAGATCCC AGAGTCCATG 
TCTGCAGAGG CCAAGGCATT CATACTGAAA TGTTTTGAAC CAGATCCTGA CAAGAGAGCC 
TGTGCTAACG ACTTGCTTGT TGATGAGTTT TTAAAAGTTT CAAGCAAAAA GAAAAAGACA 
CAACCTAAGC TTTCAGCTCT TTCAGCTGGA TCAAATGAAT ATCTCAGGAG TATATCCTTG 
CCGGTACCTG TGCTGGTGGA GGACACCAGC AGCAGCAGTG AGTACGGCTC AGTTTCACCC 
GACACGGAGT TGAAAGTGGA CCCCTTCTCT TTCAAAACAA GAGCCAAGTC CTGCGGAGAA 
AGAGATGTCA AGGGAATTCG GACACTCTTT TTGGGCATTC CAGATGAGAA TTTTGAAGAT 
CACAGTGCTC CTCCTTCCCC TGAAGAAAAA GATTCTGGAT TCTTCATGCT GAGGAAGGAC 
AGTGAGAGGC GAGCTACCCT TCACAGGATC CTGACGGAAG ACCAAGACAA AATTGTGAGA 
AACCTAATGG AATCTTTAGC TCAGGGGGCT GAAGAACCGA AACTAAAATG GGAACACATC 
ACAACCCTCA TTGCAAGCCT CAGAGAATTT GTGAGATCCA CTGACCGAAA AATCATAGCC 
ACCACACTGT CAAAGCTGAA ACTGGAGCTG GACTTCGACA GCCATGGCAT TAGCCAAGTC 
CAGGTGGTAC TCTTTGGTTT TCAAGATGCT GTCAATAAAG TTCTTCGGAA TCATAACATC 
AAGCCGCACT GGATGTTTGC CTTAGACAGT ATCATTCGGA AGGCGGTACA GACAGCCATT 
ACCATCCTGG TTCCAGAACT AAGGCCACAT TTCAGCCTTG CATCTGAGAG TGATACTGCT 
GATCAAGAAG ACTTGGATGT AGAAGATGAC CATGAGGAAC AGCCTTCAAA TCAAACTGTC 
CGAAGACCTC AGGCTGTCAT TGAAGATGCT GTGGCTACCT CAGGCGTGAG CACGCTCAGT 
TCTACTGTGT CTCATGATTC CCAGAGTGCT CACCGGTCAC TGAATGTACA GCTTGGAAGG 
ATGAAAATAG AAACCAATAG ATTACTGGAA GAATTGGTTC GGAAAGAGAA AGAATTACAA 
GCACTCCTTC ATCGAGCTAT TGAAGAAAAA GACCAAGAAA TTAAACACCT GAAGCTTAAG 
TCCCAACCCA TAGAAATTCC TGAATTGCCT GTATTTCATC TAAATTCTTC TGGCACAAAT 
ACTGAAGATT CTGAACTTAC CGACTGGCTG AGAGTGAATG GAGCTGATGA AGACACTATA 
AGCCGGTTTT TGGCTGAAGA TTATACACTA TTGGATGTTC TCTACTATGT TACACGTGAT 
GACTTAAAAT GCTTGAGACT AAGGGGAGGG ATGCTGTGCA CACTGTGGAA GGCTATCATT 
GACTTTCGAA ACAAACAGAC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_011535839.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534141.2 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.

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CloneID	OHu96612
Clone ID Related Accession (Same CDS sequence)	XM_011535839.3 , XM_011535839.2
Accession Version	XM_011535839.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011535839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGATC ATATCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534141.2
CDS	359..4219
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNAEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPDHIL

Target ORF information:

RefSeq Version	XM_011535839.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535839.3

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGATC ATATCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K5 ( XM_011535839.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011534141.2 Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.

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CloneID	OHu96612
Clone ID Related Accession (Same CDS sequence)	XM_011535839.3 , XM_011535839.2
Accession Version	XM_011535839.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3861bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 5 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000006.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011535839.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGATC ATATCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011534141.2
CDS	359..4219
Translation	MKASCRLRGRRVLSAAAAAVPGRRLGSQPARKRASGAATEAPRRSEPPPGSGVPSGERAPERRRRRGGEGAARDGSCLARRARSSPELWLARRCGWAGDAAAVAARPGEMSTEADEGITFSVPPFAPSGFCTIPEGGICRRGGAAAVGEGEEHQLPPPPPGSFWNVESAAAPGIGCPAATSSSSATRGRGSSVGGGSRRTTVAYVINEASQGQLVVAESEALQSLREACETVGATLETLHFGKLDFGETTVLDRFYNADIAVVEMSDAFRQPSLFYHLGVRESFSMANNIILYCDTNSDSLQSLKEIICQKNTMCTGNYTFVPYMITPHNKVYCCDSSFMKGLTELMQPNFELLLGPICLPLVDRFIQLLKVAQASSSQYFRESILNDIRKARNLYTGKELAAELARIRQRVDNIEVLTADIVINLLLSYRDIQDYDSIVKLVETLEKLPTFDLASHHHVKFHYAFALNRRNLPGDRAKALDIMIPMVQSEGQVASDMYCLVGRIYKDMFLDSNFTDTESRDHGASWFKKAFESEPTLQSGINYAVLLLAAGHQFESSFELRKVGVKLSSLLGKKGNLEKLQSYWEVGFFLGASVLANDHMRVIQASEKLFKLKTPAWYLKSIVETILIYKHFVKLTTEQPVAKQELVDFWMDFLVEATKTDVTVVRFPVLILEPTKIYQPSYLSINNEVEEKTISIWHVLPDDKKGIHEWNFSASSVRGVSISKFEERCCFLYVLHNSDDFQIYFCTELHCKKFFEMVNTITEEKGRSTEEGDCESDLLEYDYEYDENGDRVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKHLKHKNIVQYLGSFSENGFIKIFMEQVPGGSLSALLRSKWGPLKDNEQTIGFYTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGINPCTETFTGTLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHPEIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLKVSSKKKKTQPKLSALSAGSNAEYLRSISLPVPVLVEDTSSSSEYGSVSPDTELKVDPFSFKTRAKSCGERDVKGIRTLFLGIPDENFEDHSAPPSPEEKDSGFFMLRKDSERRATLHRILTEDQDKIVRNLMESLAQGAEEPKLKWEHITTLIASLREFVRSTDRKIIATTLSKLKLELDFDSHGISQVQVVLFGFQDAVNKVLRNHNIKPHWMFALDSIIRKAVQTAITILVPDHIL

Target ORF information:

RefSeq Version	XM_011535839.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011535839.2

ORF Insert Sequence:

ATGAAGGCGA GCTGCCGCCT AAGAGGGAGG AGGGTCCTTT CGGCTGCCGC TGCTGCTGTT 
CCGGGACGCC GCCTGGGGAG CCAGCCCGCT CGTAAGAGGG CGAGCGGCGC GGCCACGGAG 
GCGCCGAGGA GGAGCGAGCC GCCGCCGGGC AGCGGCGTGC CCTCGGGGGA GAGGGCGCCG 
GAGAGGAGGC GGCGGCGCGG CGGCGAGGGC GCGGCGCGCG ATGGCAGCTG CTTAGCCCGG 
CGGGCGCGGA GCAGCCCCGA GCTGTGGCTG GCCAGGCGGT GCGGCTGGGC GGGGGACGCC 
GCCGCCGTTG CTGCCCGGCC CGGAGAGATG AGCACGGAGG CGGACGAGGG CATCACTTTC 
TCTGTGCCAC CCTTCGCCCC CTCGGGCTTC TGCACCATCC CCGAGGGCGG CATCTGCAGG 
AGGGGAGGAG CGGCGGCGGT GGGCGAGGGC GAGGAGCACC AGCTGCCACC GCCGCCGCCG 
GGCAGCTTCT GGAACGTGGA GAGCGCCGCT GCCCCTGGCA TCGGTTGTCC GGCGGCCACC 
TCCTCGAGCA GTGCCACCCG AGGCCGGGGC AGCTCTGTTG GCGGGGGCAG CCGACGGACC 
ACGGTGGCAT ATGTGATCAA CGAAGCGAGC CAAGGGCAAC TGGTGGTGGC CGAGAGCGAG 
GCCCTGCAGA GCTTGCGGGA GGCGTGCGAG ACAGTGGGCG CCACCCTGGA AACCCTGCAT 
TTTGGGAAAC TCGACTTTGG AGAAACCACC GTGCTGGACC GCTTTTACAA TGCAGATATT 
GCGGTGGTGG AGATGAGCGA TGCCTTCCGG CAGCCGTCCT TGTTTTACCA CCTTGGGGTG 
AGAGAAAGTT TCAGCATGGC CAACAACATC ATCCTCTACT GTGATACTAA CTCGGACTCT 
CTGCAGTCAC TGAAGGAAAT AATTTGCCAG AAGAATACTA TGTGCACTGG GAACTACACC 
TTTGTTCCTT ACATGATAAC TCCACATAAC AAAGTCTACT GCTGTGACAG CAGCTTCATG 
AAGGGGTTGA CAGAGCTCAT GCAACCGAAC TTCGAGCTGC TTCTTGGACC CATCTGCTTA 
CCTCTTGTGG ATCGTTTTAT TCAACTTTTG AAGGTGGCAC AAGCAAGTTC TAGCCAGTAC 
TTCCGGGAAT CTATACTCAA TGACATCAGG AAAGCTCGTA ATTTATACAC TGGTAAAGAA 
TTGGCAGCTG AGTTGGCAAG AATTCGGCAG CGAGTAGATA ATATCGAAGT CTTGACAGCA 
GATATTGTCA TAAATCTGTT ACTTTCCTAC AGAGATATCC AGGACTATGA TTCTATTGTG 
AAGCTGGTAG AGACTTTAGA AAAACTGCCA ACCTTTGATT TGGCCTCCCA TCACCATGTG 
AAGTTTCATT ATGCATTTGC ACTGAATAGG AGAAATCTCC CTGGTGACAG AGCAAAAGCT 
CTTGATATTA TGATTCCCAT GGTGCAAAGC GAAGGACAAG TTGCTTCAGA TATGTATTGC 
CTAGTTGGTC GAATCTACAA AGATATGTTT TTGGACTCTA ATTTCACGGA CACTGAAAGC 
AGAGACCATG GAGCTTCTTG GTTCAAAAAG GCATTTGAAT CTGAGCCAAC ACTACAGTCA 
GGAATTAATT ATGCGGTCCT CCTCCTGGCA GCTGGACACC AGTTTGAATC TTCCTTTGAG 
CTCCGGAAAG TTGGGGTGAA GCTAAGTAGT CTTCTTGGTA AAAAGGGAAA CTTGGAAAAA 
CTCCAGAGCT ACTGGGAAGT TGGATTTTTT CTGGGGGCCA GCGTCCTAGC CAATGACCAC 
ATGAGAGTCA TTCAAGCATC TGAAAAGCTT TTTAAACTGA AGACACCAGC ATGGTACCTC 
AAGTCTATTG TAGAGACAAT TTTAATATAT AAGCATTTTG TGAAACTGAC CACAGAACAG 
CCTGTGGCCA AGCAAGAACT TGTGGACTTT TGGATGGATT TCCTGGTCGA GGCCACAAAG 
ACAGATGTTA CTGTGGTTAG GTTTCCAGTA TTAATATTAG AACCAACCAA AATCTATCAA 
CCTTCTTATT TGTCTATCAA CAATGAAGTT GAGGAAAAGA CAATCTCTAT TTGGCACGTG 
CTTCCTGATG ACAAGAAAGG TATACATGAG TGGAATTTTA GTGCCTCTTC TGTCAGGGGA 
GTGAGTATTT CTAAATTTGA AGAAAGATGC TGCTTTCTTT ATGTGCTTCA CAATTCTGAT 
GATTTCCAAA TCTATTTCTG TACAGAACTT CATTGTAAAA AGTTTTTTGA GATGGTGAAC 
ACCATTACCG AAGAGAAGGG GAGAAGCACA GAGGAAGGAG ACTGTGAAAG TGACTTGCTG 
GAGTATGACT ATGAATATGA TGAAAATGGT GACAGAGTCG TTTTAGGAAA AGGCACTTAT 
GGGATAGTCT ACGCAGGTCG GGACTTGAGC AACCAAGTCA GAATTGCTAT TAAGGAAATC 
CCAGAGAGAG ACAGCAGATA CTCTCAGCCC CTGCATGAAG AAATAGCATT GCATAAACAC 
CTGAAGCACA AAAATATTGT CCAGTATCTG GGCTCTTTCA GTGAGAATGG TTTCATTAAA 
ATCTTCATGG AGCAGGTCCC TGGAGGAAGT CTTTCTGCTC TCCTTCGTTC CAAATGGGGT 
CCATTAAAAG ACAATGAGCA AACAATTGGC TTTTATACAA AGCAAATACT GGAAGGATTA 
AAATATCTCC ATGACAATCA GATAGTTCAC CGGGACATAA AGGGTGACAA TGTGTTGATT 
AATACCTACA GTGGTGTTCT CAAGATCTCT GACTTCGGAA CATCAAAGAG GCTTGCTGGC 
ATAAACCCCT GTACTGAAAC TTTTACTGGT ACCCTCCAGT ATATGGCACC AGAAATAATA 
GATAAAGGAC CAAGAGGCTA CGGAAAAGCA GCAGACATCT GGTCTCTGGG CTGTACAATC 
ATTGAAATGG CCACAGGAAA ACCCCCATTT TATGAACTGG GAGAACCACA AGCAGCTATG 
TTCAAGGTGG GAATGTTTAA AGTCCACCCT GAGATCCCAG AGTCCATGTC TGCAGAGGCC 
AAGGCATTCA TACTGAAATG TTTTGAACCA GATCCTGACA AGAGAGCCTG TGCTAACGAC 
TTGCTTGTTG ATGAGTTTTT AAAAGTTTCA AGCAAAAAGA AAAAGACACA ACCTAAGCTT 
TCAGCTCTTT CAGCTGGATC AAATGCAGAA TATCTCAGGA GTATATCCTT GCCGGTACCT 
GTGCTGGTGG AGGACACCAG CAGCAGCAGT GAGTACGGCT CAGTTTCACC CGACACGGAG 
TTGAAAGTGG ACCCCTTCTC TTTCAAAACA AGAGCCAAGT CCTGCGGAGA AAGAGATGTC 
AAGGGAATTC GGACACTCTT TTTGGGCATT CCAGATGAGA ATTTTGAAGA TCACAGTGCT 
CCTCCTTCCC CTGAAGAAAA AGATTCTGGA TTCTTCATGC TGAGGAAGGA CAGTGAGAGG 
CGAGCTACCC TTCACAGGAT CCTGACGGAA GACCAAGACA AAATTGTGAG AAACCTAATG 
GAATCTTTAG CTCAGGGGGC TGAAGAACCG AAACTAAAAT GGGAACACAT CACAACCCTC 
ATTGCAAGCC TCAGAGAATT TGTGAGATCC ACTGACCGAA AAATCATAGC CACCACACTG 
TCAAAGCTGA AACTGGAGCT GGACTTCGAC AGCCATGGCA TTAGCCAAGT CCAGGTGGTA 
CTCTTTGGTT TTCAAGATGC TGTCAATAAA GTTCTTCGGA ATCATAACAT CAAGCCGCAC 
TGGATGTTTG CCTTAGACAG TATCATTCGG AAGGCGGTAC AGACAGCCAT TACCATCCTG 
GTTCCAGATC ATATCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	MAP3K5
Entrez Gene ID	4217
Full Name	mitogen-activated protein kinase kinase kinase 5
Synonyms	ASK1,MAPKKK5,MEKK5
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 5 Names mitogen-activated protein kinase kinase kinase 5 ASK-1 MAP/ERK kinase kinase 5 MAPK/ERK kinase kinase 5 MEK kinase 5 MEKK 5 apoptosis signal-regulating kinase 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 6 Map Location: 6q23.3
Summary	Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells; MAPKKK5 does not activate MAPK/ERK. [provided by RefSeq, Jul 2008].
Disorder MIM:	602448

Pan troglodytes (chimpanzee)	MAP3K5	XP_001171211.1
	MAP3K5	XP_002803972.1
Mus musculus (house mouse)	Map3k5	NP_032606.4
Danio rerio (zebrafish)	map3k5	NP_001155222.1
Homo sapiens (human)	MAP3K5	NP_005914.1
Rattus norvegicus (Norway rat)	Map3k5	NP_001264623.1
Gallus gallus (chicken)	MAP3K5	XP_419725.3
Xenopus tropicalis (tropical clawed frog)	LOC100497692	XP_002936311.2
Canis lupus familiaris (dog)	MAP3K5	XP_533420.4
Bos taurus (cattle)	MAP3K5	NP_001137553.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

MAP3K5 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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