MEFV cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MEFV
Entrez Gene ID	4210
Full Name	MEFV, pyrin innate immunity regulator
Synonyms	FMF,MEF,TRIM20
General protein information	Preferred Names MEFV, pyrin innate immunity regulator Names pyrin Mediterranean fever marenostrin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p13.3
Summary	This gene encodes a protein, also known as pyrin or marenostrin, that is an important modulator of innate immunity. Mutations in this gene are associated with Mediterranean fever, a hereditary periodic fever syndrome. [provided by RefSeq, Jul 2008].
Disorder MIM:	608107
Disorder Html:	Familial Mediterranean fever, AR, 249100 (3); Familial Mediterranean fever, AD, 134610 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017023236.2	XP_016878725.1	pyrin isoform X1
XM_017023236.1	XP_016878725.1	pyrin isoform X1
NM_001198536.1	NP_001185465.1	pyrin isoform 2
NM_000243.2	NP_000234.1	pyrin isoform 1
NM_001198536.2	NP_001185465.2	pyrin isoform 2
NM_000243.3	NP_000234.1	pyrin isoform 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MEFV	XP_523280.3
Mus musculus (house mouse)	Mefv	NP_001155263.1
Bos taurus (cattle)	LOC529195	XP_002697924.3
Homo sapiens (human)	MEFV	NP_000234.1
	MEFV	XP_001092338.1
Rattus norvegicus (Norway rat)	Mefv	NP_113822.1
Canis lupus familiaris (dog)	MEFV	XP_547161.4

Gene Ontology

The following MEFV gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MEFV cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19174	NM_000243.2 Latest version!	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu21586	NM_001198536.1 Latest version!	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
OHu109360	NM_001198536.2 Latest version!	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu109378	NM_000243.3 Latest version!	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu86035	XM_017023236.2 Latest version!	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu86035	XM_017023236.1	Homo sapiens Mediterranean fever (MEFV), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MEFV ( NM_000243.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000234.1 Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 1, mRNA.

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CloneID	OHu19174
Clone ID Related Accession (Same CDS sequence)	NM_000243.2
Accession Version	NM_000243.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-27
Organism	Homo sapiens(human)
Product	pyrin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF018080.1 and AJ003147.1. This sequence is a reference standard in the RefSeqGene project. On May 15, 2008 this sequence version replaced NM_000243.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018080.1, Y14441.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAGAAAATTC AGAAGCAGCT GGAGCATCTG AAGAAGCTGA GAAAATCAGG GGAGGAGCAG 
CGATCCTATG GGGAGGAGAA GGCAGTGAGC TTTCTGAAAC AAACTGAAGC GCTGAAGCAG 
CGGGTGCAGA GGAAGCTGGA GCAGGTGTAC TACTTCCTGG AGCAGCAAGA GCATTTCTTT 
GTGGCCTCAC TGGAGGACGT GGGCCAGATG GTTGGGCAGA TCAGGAAGGC ATATGACACC 
CGCGTATCCC AGGACATCGC CCTGCTCGAT GCGCTGATTG GGGAACTGGA GGCCAAGGAG 
TGCCAGTCAG AATGGGAACT TCTGCAGGAC ATTGGAGACA TCTTGCACAG GGCTAAGACA 
GTGCCTGTCC CTGAAAAGTG GACCACTCCT CAAGAGATAA AACAAAAGAT CCAACTCCTC 
CACCAGAAGT CAGAGTTTGT GGAGAAGAGC ACAAAGTACT TCTCAGAAAC CCTGCGTTCA 
GAAATGGAAA TGTTCAATGT TCCAGAGCTG ATTGGCGCTC AGGCACATGC TGTTAATGTG 
ATTCTGGATG CAGAAACCGC TTACCCCAAC CTCATCTTCT CTGATGATCT GAAGAGTGTT 
AGACTTGGAA ACAAGTGGGA GAGGCTGCCT GATGGCCCGC AAAGATTTGA CAGCTGTATC 
ATTGTTCTGG GCTCTCCGAG TTTCCTCTCT GGCCGCCGTT ACTGGGAGGT GGAGGTTGGA 
GACAAGACAG CATGGATCCT GGGAGCCTGC AAGACATCCA TAAGCAGGAA AGGGAACATG 
ACTCTGTCGC CAGAGAATGG CTACTGGGTG GTGATAATGA TGAAGGAAAA TGAGTACCAG 
GCGTCCAGCG TTCCCCCGAC CCGCCTGCTA ATAAAGGAGC CTCCCAAGCG TGTGGGCATC 
TTCGTGGACT ACAGAGTTGG AAGCATCTCC TTTTACAATG TGACAGCCAG ATCCCACATC 
TATACATTCG CCAGCTGCTC TTTCTCTGGG CCCCTTCAAC CTATCTTCAG CCCTGGGACA 
CGTGATGGAG GGAAGAACAC AGCTCCTCTG ACTATCTGTC CAGTGGGTGG TCAGGGGCCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000234.1
CDS	41..2386
Translation	MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVRLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPLTICPVGGQGPD

Target ORF information:

RefSeq Version	NM_000243.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000243.2

ORF Insert Sequence:

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAGAAAATTC AGAAGCAGCT GGAGCATCTG AAGAAGCTGA GAAAATCAGG GGAGGAGCAG 
CGATCCTATG GGGAGGAGAA GGCAGTGAGC TTTCTGAAAC AAACTGAAGC GCTGAAGCAG 
CGGGTGCAGA GGAAGCTGGA GCAGGTGTAC TACTTCCTGG AGCAGCAAGA GCATTTCTTT 
GTGGCCTCAC TGGAGGACGT GGGCCAGATG GTTGGGCAGA TCAGGAAGGC ATATGACACC 
CGCGTATCCC AGGACATCGC CCTGCTCGAT GCGCTGATTG GGGAACTGGA GGCCAAGGAG 
TGCCAGTCAG AATGGGAACT TCTGCAGGAC ATTGGAGACA TCTTGCACAG GGCTAAGACA 
GTGCCTGTCC CTGAAAAGTG GACCACTCCT CAAGAGATAA AACAAAAGAT CCAACTCCTC 
CACCAGAAGT CAGAGTTTGT GGAGAAGAGC ACAAAGTACT TCTCAGAAAC CCTGCGTTCA 
GAAATGGAAA TGTTCAATGT TCCAGAGCTG ATTGGCGCTC AGGCACATGC TGTTAATGTG 
ATTCTGGATG CAGAAACCGC TTACCCCAAC CTCATCTTCT CTGATGATCT GAAGAGTGTT 
AGACTTGGAA ACAAGTGGGA GAGGCTGCCT GATGGCCCGC AAAGATTTGA CAGCTGTATC 
ATTGTTCTGG GCTCTCCGAG TTTCCTCTCT GGCCGCCGTT ACTGGGAGGT GGAGGTTGGA 
GACAAGACAG CATGGATCCT GGGAGCCTGC AAGACATCCA TAAGCAGGAA AGGGAACATG 
ACTCTGTCGC CAGAGAATGG CTACTGGGTG GTGATAATGA TGAAGGAAAA TGAGTACCAG 
GCGTCCAGCG TTCCCCCGAC CCGCCTGCTA ATAAAGGAGC CTCCCAAGCG TGTGGGCATC 
TTCGTGGACT ACAGAGTTGG AAGCATCTCC TTTTACAATG TGACAGCCAG ATCCCACATC 
TATACATTCG CCAGCTGCTC TTTCTCTGGG CCCCTTCAAC CTATCTTCAG CCCTGGGACA 
CGTGATGGAG GGAAGAACAC AGCTCCTCTG ACTATCTGTC CAGTGGGTGG TCAGGGGCCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEFV ( NM_001198536.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185465.1 Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu21586
Clone ID Related Accession (Same CDS sequence)	NM_001198536.1
Accession Version	NM_001198536.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-27
Organism	Homo sapiens(human)
Product	pyrin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF018080.1, FJ785722.1, Y14441.1 and AJ003147.1. Transcript Variant: This variant lacks an alternate in-frame exon and uses an alternate splice junction at the 5' end of an exon, that causes a frameshift. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ785722.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGGAA GGCCACCAGA CACGGCTGCG 
AGTCCCCGCT GCCACGCCCA GGAAGGAGAC CCAGTTGACG GTACCTGTGT GCGTGATTCC 
TGCAGCTTCC CCGAGGCAGT TTCTGGGCAC CCCCAGGCCT CAGGCAGCCG CTCACCTGGC 
TGCCCCCGGT GCCAGGACTC CCATGAAAGG AAGAGCCCGG GAAGCCTAAG CCCCCAGCCC 
CTGCCACAGT GTAAGCGCCA CCTGAAGCAG GTCCAGCTGC TCTTCTGTGA GGATCACGAT 
GAGCCCATCT GCCTCATCTG CAGTCTGAGT CAGGAGCACC AAGGCCACCG GGTGCGCCCC 
ATTGAGGAGG TCGCCCTGGA ACACAAGAAG AAAATTCAGA AGCAGCTGGA GCATCTGAAG 
AAGCTGAGAA AATCAGGGGA GGAGCAGCGA TCCTATGGGG AGGAGAAGGC AGTGAGCTTT 
CTGAAACAAA CTGAAGCGCT GAAGCAGCGG GTGCAGAGGA AGCTGGAGCA GGTGTACTAC 
TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG GCCTCACTGG AGGACGTGGG CCAGATGGTT 
GGGCAGATCA GGAAGGCATA TGACACCCGC GTATCCCAGG ACATCGCCCT GCTCGATGCG 
CTGATTGGGG AACTGGAGGC CAAGGAGTGC CAGTCAGAAT GGGAACTTCT GCAGGACATT 
GGAGACATCT TGCACAGGGC TAAGACAGTG CCTGTCCCTG AAAAGTGGAC CACTCCTCAA 
GAGATAAAAC AAAAGATCCA ACTCCTCCAC CAGAAGTCAG AGTTTGTGGA GAAGAGCACA 
AAGTACTTCT CAGAAACCCT GCGTTCAGAA ATGGAAATGT TCAATGACCA CAGTCCCCAA 
CATGGCCTGG GTTCCTGGGA GGAACGGGAT TATACCCAAC ATAGCATGCA GGGCCCTAAG 
CAGGGGGTTC CTTGTCTTTC CTTGTTGTCA GGACAGTGTA ATTTAGCCCC TCTTAATGCT 
AATGCTCAGG ATTTTTTTCC CTATCTGATT TTTCTCCGTA GTTCCAGAGC TGATTGGCGC 
TCAGGCACAT GCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185465.1
CDS	41..1378
Translation	MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNDHSPQHGLGSWEERDYTQHSMQGPKQGVPCLSLLSGQCNLAPLNANAQDFFPYLIFLRSSRADWRSGTCC

Target ORF information:

RefSeq Version	NM_001198536.1
Organism	Homo sapiens(human)
Definition	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198536.1

ORF Insert Sequence:

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGGAA GGCCACCAGA CACGGCTGCG 
AGTCCCCGCT GCCACGCCCA GGAAGGAGAC CCAGTTGACG GTACCTGTGT GCGTGATTCC 
TGCAGCTTCC CCGAGGCAGT TTCTGGGCAC CCCCAGGCCT CAGGCAGCCG CTCACCTGGC 
TGCCCCCGGT GCCAGGACTC CCATGAAAGG AAGAGCCCGG GAAGCCTAAG CCCCCAGCCC 
CTGCCACAGT GTAAGCGCCA CCTGAAGCAG GTCCAGCTGC TCTTCTGTGA GGATCACGAT 
GAGCCCATCT GCCTCATCTG CAGTCTGAGT CAGGAGCACC AAGGCCACCG GGTGCGCCCC 
ATTGAGGAGG TCGCCCTGGA ACACAAGAAG AAAATTCAGA AGCAGCTGGA GCATCTGAAG 
AAGCTGAGAA AATCAGGGGA GGAGCAGCGA TCCTATGGGG AGGAGAAGGC AGTGAGCTTT 
CTGAAACAAA CTGAAGCGCT GAAGCAGCGG GTGCAGAGGA AGCTGGAGCA GGTGTACTAC 
TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG GCCTCACTGG AGGACGTGGG CCAGATGGTT 
GGGCAGATCA GGAAGGCATA TGACACCCGC GTATCCCAGG ACATCGCCCT GCTCGATGCG 
CTGATTGGGG AACTGGAGGC CAAGGAGTGC CAGTCAGAAT GGGAACTTCT GCAGGACATT 
GGAGACATCT TGCACAGGGC TAAGACAGTG CCTGTCCCTG AAAAGTGGAC CACTCCTCAA 
GAGATAAAAC AAAAGATCCA ACTCCTCCAC CAGAAGTCAG AGTTTGTGGA GAAGAGCACA 
AAGTACTTCT CAGAAACCCT GCGTTCAGAA ATGGAAATGT TCAATGACCA CAGTCCCCAA 
CATGGCCTGG GTTCCTGGGA GGAACGGGAT TATACCCAAC ATAGCATGCA GGGCCCTAAG 
CAGGGGGTTC CTTGTCTTTC CTTGTTGTCA GGACAGTGTA ATTTAGCCCC TCTTAATGCT 
AATGCTCAGG ATTTTTTTCC CTATCTGATT TTTCTCCGTA GTTCCAGAGC TGATTGGCGC 
TCAGGCACAT GCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEFV ( NM_001198536.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001185465.2 Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu109360
Clone ID Related Accession (Same CDS sequence)	NM_001198536.2
Accession Version	NM_001198536.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	pyrin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ003147.1, AF018080.1, FJ785722.1 and Y14441.1. On Dec 30, 2019 this sequence version replaced NM_001198536.1. Transcript Variant: This variant (2) lacks an alternate in-frame exon and uses an alternate splice junction at the 5' end of an exon, that causes a frameshift. The resulting isoform (2) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: FJ785722.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGGAA GGCCACCAGA CACGGCTGCG 
AGTCCCCGCT GCCACGCCCA GGAAGGAGAC CCAGTTGACG GTACCTGTGT GCGTGATTCC 
TGCAGCTTCC CCGAGGCAGT TTCTGGGCAC CCCCAGGCCT CAGGCAGCCG CTCACCTGGC 
TGCCCCCGGT GCCAGGACTC CCATGAAAGG AAGAGCCCGG GAAGCCTAAG CCCCCAGCCC 
CTGCCACAGT GTAAGCGCCA CCTGAAGCAG GTCCAGCTGC TCTTCTGTGA GGATCACGAT 
GAGCCCATCT GCCTCATCTG CAGTCTGAGT CAGGAGCACC AAGGCCACCG GGTGCGCCCC 
ATTGAGGAGG TCGCCCTGGA ACACAAGAAG AAAATTCAGA AGCAGCTGGA GCATCTGAAG 
AAGCTGAGAA AATCAGGGGA GGAGCAGCGA TCCTATGGGG AGGAGAAGGC AGTGAGCTTT 
CTGAAACAAA CTGAAGCGCT GAAGCAGCGG GTGCAGAGGA AGCTGGAGCA GGTGTACTAC 
TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG GCCTCACTGG AGGACGTGGG CCAGATGGTT 
GGGCAGATCA GGAAGGCATA TGACACCCGC GTATCCCAGG ACATCGCCCT GCTCGATGCG 
CTGATTGGGG AACTGGAGGC CAAGGAGTGC CAGTCAGAAT GGGAACTTCT GCAGGACATT 
GGAGACATCT TGCACAGGGC TAAGACAGTG CCTGTCCCTG AAAAGTGGAC CACTCCTCAA 
GAGATAAAAC AAAAGATCCA ACTCCTCCAC CAGAAGTCAG AGTTTGTGGA GAAGAGCACA 
AAGTACTTCT CAGAAACCCT GCGTTCAGAA ATGGAAATGT TCAATGACCA CAGTCCCCAA 
CATGGCCTGG GTTCCTGGGA GGAACGGGAT TATACCCAAC ATAGCATGCA GGGCCCTAAG 
CAGGGGGTTC CTTGTCTTTC CTTGTTGTCA GGACAGTGTA ATTTAGCCCC TCTTAATGCT 
AATGCTCAGG ATTTTTTTCC CTATCTGATT TTTCTCCGTA GTTCCGGAGC TGATTGGCGC 
TCAGGCACAT GCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001185465.2
CDS	47..1384
Translation	MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNDHSPQHGLGSWEERDYTQHSMQGPKQGVPCLSLLSGQCNLAPLNANAQDFFPYLIFLRSSGADWRSGTCC

Target ORF information:

RefSeq Version	NM_001198536.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001198536.2

ORF Insert Sequence:

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGGAA GGCCACCAGA CACGGCTGCG 
AGTCCCCGCT GCCACGCCCA GGAAGGAGAC CCAGTTGACG GTACCTGTGT GCGTGATTCC 
TGCAGCTTCC CCGAGGCAGT TTCTGGGCAC CCCCAGGCCT CAGGCAGCCG CTCACCTGGC 
TGCCCCCGGT GCCAGGACTC CCATGAAAGG AAGAGCCCGG GAAGCCTAAG CCCCCAGCCC 
CTGCCACAGT GTAAGCGCCA CCTGAAGCAG GTCCAGCTGC TCTTCTGTGA GGATCACGAT 
GAGCCCATCT GCCTCATCTG CAGTCTGAGT CAGGAGCACC AAGGCCACCG GGTGCGCCCC 
ATTGAGGAGG TCGCCCTGGA ACACAAGAAG AAAATTCAGA AGCAGCTGGA GCATCTGAAG 
AAGCTGAGAA AATCAGGGGA GGAGCAGCGA TCCTATGGGG AGGAGAAGGC AGTGAGCTTT 
CTGAAACAAA CTGAAGCGCT GAAGCAGCGG GTGCAGAGGA AGCTGGAGCA GGTGTACTAC 
TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG GCCTCACTGG AGGACGTGGG CCAGATGGTT 
GGGCAGATCA GGAAGGCATA TGACACCCGC GTATCCCAGG ACATCGCCCT GCTCGATGCG 
CTGATTGGGG AACTGGAGGC CAAGGAGTGC CAGTCAGAAT GGGAACTTCT GCAGGACATT 
GGAGACATCT TGCACAGGGC TAAGACAGTG CCTGTCCCTG AAAAGTGGAC CACTCCTCAA 
GAGATAAAAC AAAAGATCCA ACTCCTCCAC CAGAAGTCAG AGTTTGTGGA GAAGAGCACA 
AAGTACTTCT CAGAAACCCT GCGTTCAGAA ATGGAAATGT TCAATGACCA CAGTCCCCAA 
CATGGCCTGG GTTCCTGGGA GGAACGGGAT TATACCCAAC ATAGCATGCA GGGCCCTAAG 
CAGGGGGTTC CTTGTCTTTC CTTGTTGTCA GGACAGTGTA ATTTAGCCCC TCTTAATGCT 
AATGCTCAGG ATTTTTTTCC CTATCTGATT TTTCTCCGTA GTTCCGGAGC TGATTGGCGC 
TCAGGCACAT GCTGTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEFV ( NM_000243.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000234.1 Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 1, mRNA.

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CloneID	OHu109378
Clone ID Related Accession (Same CDS sequence)	NM_000243.3
Accession Version	NM_000243.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-02
Organism	Homo sapiens(human)
Product	pyrin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ003147.1, AF018080.1 and AK298660.1. On Dec 30, 2019 this sequence version replaced NM_000243.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF018080.1, Y14441.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAGAAAATTC AGAAGCAGCT GGAGCATCTG AAGAAGCTGA GAAAATCAGG GGAGGAGCAG 
CGATCCTATG GGGAGGAGAA GGCAGTGAGC TTTCTGAAAC AAACTGAAGC GCTGAAGCAG 
CGGGTGCAGA GGAAGCTGGA GCAGGTGTAC TACTTCCTGG AGCAGCAAGA GCATTTCTTT 
GTGGCCTCAC TGGAGGACGT GGGCCAGATG GTTGGGCAGA TCAGGAAGGC ATATGACACC 
CGCGTATCCC AGGACATCGC CCTGCTCGAT GCGCTGATTG GGGAACTGGA GGCCAAGGAG 
TGCCAGTCAG AATGGGAACT TCTGCAGGAC ATTGGAGACA TCTTGCACAG GGCTAAGACA 
GTGCCTGTCC CTGAAAAGTG GACCACTCCT CAAGAGATAA AACAAAAGAT CCAACTCCTC 
CACCAGAAGT CAGAGTTTGT GGAGAAGAGC ACAAAGTACT TCTCAGAAAC CCTGCGTTCA 
GAAATGGAAA TGTTCAATGT TCCGGAGCTG ATTGGCGCTC AGGCACATGC TGTTAATGTG 
ATTCTGGATG CAGAAACCGC TTACCCCAAC CTCATCTTCT CTGATGATCT GAAGAGTGTT 
AGACTTGGAA ACAAGTGGGA GAGGCTGCCT GATGGCCCGC AAAGATTTGA CAGCTGTATC 
ATTGTTCTGG GCTCTCCGAG TTTCCTCTCT GGCCGCCGTT ACTGGGAGGT GGAGGTTGGA 
GACAAGACAG CATGGATCCT GGGAGCCTGC AAGACATCCA TAAGCAGGAA AGGGAACATG 
ACTCTGTCGC CAGAGAATGG CTACTGGGTG GTGATAATGA TGAAGGAAAA TGAGTACCAG 
GCGTCCAGCG TTCCCCCGAC CCGCCTGCTA ATAAAGGAGC CTCCCAAGCG TGTGGGCATC 
TTCGTGGACT ACAGAGTTGG AAGCATCTCC TTTTACAATG TGACAGCCAG ATCCCACATC 
TATACATTCG CCAGCTGCTC TTTCTCTGGG CCCCTTCAAC CTATCTTCAG CCCTGGGACA 
CGTGATGGAG GGAAGAACAC AGCTCCTCTG ACTATCTGTC CAGTGGGTGG TCAGGGGCCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000234.1
CDS	47..2392
Translation	MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVRLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPLTICPVGGQGPD

Target ORF information:

RefSeq Version	NM_000243.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000243.3

ORF Insert Sequence:

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAGAAAATTC AGAAGCAGCT GGAGCATCTG AAGAAGCTGA GAAAATCAGG GGAGGAGCAG 
CGATCCTATG GGGAGGAGAA GGCAGTGAGC TTTCTGAAAC AAACTGAAGC GCTGAAGCAG 
CGGGTGCAGA GGAAGCTGGA GCAGGTGTAC TACTTCCTGG AGCAGCAAGA GCATTTCTTT 
GTGGCCTCAC TGGAGGACGT GGGCCAGATG GTTGGGCAGA TCAGGAAGGC ATATGACACC 
CGCGTATCCC AGGACATCGC CCTGCTCGAT GCGCTGATTG GGGAACTGGA GGCCAAGGAG 
TGCCAGTCAG AATGGGAACT TCTGCAGGAC ATTGGAGACA TCTTGCACAG GGCTAAGACA 
GTGCCTGTCC CTGAAAAGTG GACCACTCCT CAAGAGATAA AACAAAAGAT CCAACTCCTC 
CACCAGAAGT CAGAGTTTGT GGAGAAGAGC ACAAAGTACT TCTCAGAAAC CCTGCGTTCA 
GAAATGGAAA TGTTCAATGT TCCGGAGCTG ATTGGCGCTC AGGCACATGC TGTTAATGTG 
ATTCTGGATG CAGAAACCGC TTACCCCAAC CTCATCTTCT CTGATGATCT GAAGAGTGTT 
AGACTTGGAA ACAAGTGGGA GAGGCTGCCT GATGGCCCGC AAAGATTTGA CAGCTGTATC 
ATTGTTCTGG GCTCTCCGAG TTTCCTCTCT GGCCGCCGTT ACTGGGAGGT GGAGGTTGGA 
GACAAGACAG CATGGATCCT GGGAGCCTGC AAGACATCCA TAAGCAGGAA AGGGAACATG 
ACTCTGTCGC CAGAGAATGG CTACTGGGTG GTGATAATGA TGAAGGAAAA TGAGTACCAG 
GCGTCCAGCG TTCCCCCGAC CCGCCTGCTA ATAAAGGAGC CTCCCAAGCG TGTGGGCATC 
TTCGTGGACT ACAGAGTTGG AAGCATCTCC TTTTACAATG TGACAGCCAG ATCCCACATC 
TATACATTCG CCAGCTGCTC TTTCTCTGGG CCCCTTCAAC CTATCTTCAG CCCTGGGACA 
CGTGATGGAG GGAAGAACAC AGCTCCTCTG ACTATCTGTC CAGTGGGTGG TCAGGGGCCT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEFV ( XM_017023236.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878725.1 Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant X1, mRNA.

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CloneID	OHu86035
Clone ID Related Accession (Same CDS sequence)	XM_017023236.2 , XM_017023236.1
Accession Version	XM_017023236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	pyrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017023236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAAATTCAGA AGCAGCTGGA GCATCTGAAG AAGCTGAGAA AATCAGGGGA GGAGCAGCGA 
TCCTATGGGG AGGAGAAGGC AGTGAGCTTT CTGAAACAAA CTGAAGCGCT GAAGCAGCGG 
GTGCAGAGGA AGCTGGAGCA GGTGTACTAC TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG 
GCCTCACTGG AGGACGTGGG CCAGATGGTT GGGCAGATCA GGAAGGCATA TGACACCCGC 
GTATCCCAGG ACATCGCCCT GCTCGATGCG CTGATTGGGG AACTGGAGGC CAAGGAGTGC 
CAGTCAGAAT GGGAACTTCT GCAGGACATT GGAGACATCT TGCACAGGGC TAAGACAGTG 
CCTGTCCCTG AAAAGTGGAC CACTCCTCAA GAGATAAAAC AAAAGATCCA ACTCCTCCAC 
CAGAAGTCAG AGTTTGTGGA GAAGAGCACA AAGTACTTCT CAGAAACCCT GCGTTCAGAA 
ATGGAAATGT TCAATGTTCC GGAGCTGATT GGCGCTCAGG CACATGCTGT TAATGTGATT 
CTGGATGCAG AAACCGCTTA CCCCAACCTC ATCTTCTCTG ATGATCTGAA GAGTGTTAGA 
CTTGGAAACA AGTGGGAGAG GCTGCCTGAT GGCCCGCAAA GATTTGACAG CTGTATCATT 
GTTCTGGGCT CTCCGAGTTT CCTCTCTGGC CGCCGTTACT GGGAGGTGGA GGTTGGAGAC 
AAGACAGCAT GGATCCTGGG AGCCTGCAAG ACATCCATAA GCAGGAAAGG GAACATGACT 
CTGTCGCCAG AGAATGGCTA CTGGGTGGTG ATAATGATGA AGGAAAATGA GTACCAGGCG 
TCCAGCGTTC CCCCGACCCG CCTGCTAATA AAGGAGCCTC CCAAGCGTGT GGGCATCTTC 
GTGGACTACA GAGTTGGAAG CATCTCCTTT TACAATGTGA CAGCCAGATC CCACATCTAT 
ACATTCGCCA GCTGCTCTTT CTCTGGGCCC CTTCAACCTA TCTTCAGCCC TGGGACACGT 
GATGGAGGGA AGAACACAGC TCCTCTGACT ATCTGTCCAG TGGGTGGTCA GGGGCCTGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878725.1
CDS	183..2525
Translation	MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVRLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPLTICPVGGQGPD

Target ORF information:

RefSeq Version	XM_017023236.2
Organism	Homo sapiens(human)
Definition	Homo sapiens MEFV innate immuity regulator, pyrin (MEFV), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023236.2

ORF Insert Sequence:

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAAATTCAGA AGCAGCTGGA GCATCTGAAG AAGCTGAGAA AATCAGGGGA GGAGCAGCGA 
TCCTATGGGG AGGAGAAGGC AGTGAGCTTT CTGAAACAAA CTGAAGCGCT GAAGCAGCGG 
GTGCAGAGGA AGCTGGAGCA GGTGTACTAC TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG 
GCCTCACTGG AGGACGTGGG CCAGATGGTT GGGCAGATCA GGAAGGCATA TGACACCCGC 
GTATCCCAGG ACATCGCCCT GCTCGATGCG CTGATTGGGG AACTGGAGGC CAAGGAGTGC 
CAGTCAGAAT GGGAACTTCT GCAGGACATT GGAGACATCT TGCACAGGGC TAAGACAGTG 
CCTGTCCCTG AAAAGTGGAC CACTCCTCAA GAGATAAAAC AAAAGATCCA ACTCCTCCAC 
CAGAAGTCAG AGTTTGTGGA GAAGAGCACA AAGTACTTCT CAGAAACCCT GCGTTCAGAA 
ATGGAAATGT TCAATGTTCC GGAGCTGATT GGCGCTCAGG CACATGCTGT TAATGTGATT 
CTGGATGCAG AAACCGCTTA CCCCAACCTC ATCTTCTCTG ATGATCTGAA GAGTGTTAGA 
CTTGGAAACA AGTGGGAGAG GCTGCCTGAT GGCCCGCAAA GATTTGACAG CTGTATCATT 
GTTCTGGGCT CTCCGAGTTT CCTCTCTGGC CGCCGTTACT GGGAGGTGGA GGTTGGAGAC 
AAGACAGCAT GGATCCTGGG AGCCTGCAAG ACATCCATAA GCAGGAAAGG GAACATGACT 
CTGTCGCCAG AGAATGGCTA CTGGGTGGTG ATAATGATGA AGGAAAATGA GTACCAGGCG 
TCCAGCGTTC CCCCGACCCG CCTGCTAATA AAGGAGCCTC CCAAGCGTGT GGGCATCTTC 
GTGGACTACA GAGTTGGAAG CATCTCCTTT TACAATGTGA CAGCCAGATC CCACATCTAT 
ACATTCGCCA GCTGCTCTTT CTCTGGGCCC CTTCAACCTA TCTTCAGCCC TGGGACACGT 
GATGGAGGGA AGAACACAGC TCCTCTGACT ATCTGTCCAG TGGGTGGTCA GGGGCCTGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MEFV ( XM_017023236.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878725.1 Homo sapiens Mediterranean fever (MEFV), transcript variant X1, mRNA.

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CloneID	OHu86035
Clone ID Related Accession (Same CDS sequence)	XM_017023236.2 , XM_017023236.1
Accession Version	XM_017023236.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	pyrin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAAATTCAGA AGCAGCTGGA GCATCTGAAG AAGCTGAGAA AATCAGGGGA GGAGCAGCGA 
TCCTATGGGG AGGAGAAGGC AGTGAGCTTT CTGAAACAAA CTGAAGCGCT GAAGCAGCGG 
GTGCAGAGGA AGCTGGAGCA GGTGTACTAC TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG 
GCCTCACTGG AGGACGTGGG CCAGATGGTT GGGCAGATCA GGAAGGCATA TGACACCCGC 
GTATCCCAGG ACATCGCCCT GCTCGATGCG CTGATTGGGG AACTGGAGGC CAAGGAGTGC 
CAGTCAGAAT GGGAACTTCT GCAGGACATT GGAGACATCT TGCACAGGGC TAAGACAGTG 
CCTGTCCCTG AAAAGTGGAC CACTCCTCAA GAGATAAAAC AAAAGATCCA ACTCCTCCAC 
CAGAAGTCAG AGTTTGTGGA GAAGAGCACA AAGTACTTCT CAGAAACCCT GCGTTCAGAA 
ATGGAAATGT TCAATGTTCC GGAGCTGATT GGCGCTCAGG CACATGCTGT TAATGTGATT 
CTGGATGCAG AAACCGCTTA CCCCAACCTC ATCTTCTCTG ATGATCTGAA GAGTGTTAGA 
CTTGGAAACA AGTGGGAGAG GCTGCCTGAT GGCCCGCAAA GATTTGACAG CTGTATCATT 
GTTCTGGGCT CTCCGAGTTT CCTCTCTGGC CGCCGTTACT GGGAGGTGGA GGTTGGAGAC 
AAGACAGCAT GGATCCTGGG AGCCTGCAAG ACATCCATAA GCAGGAAAGG GAACATGACT 
CTGTCGCCAG AGAATGGCTA CTGGGTGGTG ATAATGATGA AGGAAAATGA GTACCAGGCG 
TCCAGCGTTC CCCCGACCCG CCTGCTAATA AAGGAGCCTC CCAAGCGTGT GGGCATCTTC 
GTGGACTACA GAGTTGGAAG CATCTCCTTT TACAATGTGA CAGCCAGATC CCACATCTAT 
ACATTCGCCA GCTGCTCTTT CTCTGGGCCC CTTCAACCTA TCTTCAGCCC TGGGACACGT 
GATGGAGGGA AGAACACAGC TCCTCTGACT ATCTGTCCAG TGGGTGGTCA GGGGCCTGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878725.1
CDS	182..2524
Translation	MAKTPSDHLLSTLEELVPYDFEKFKFKLQNTSVQKEHSRIPRSQIQRARPVKMATLLVTYYGEEYAVQLTLQVLRAINQRLLAEELHRAAIQEYSTQENGTDDSAASSSLGENKPRSLKTPDHPEGNEGNGPRPYGGGAASLRCSQPEAGRGLSRKPLSKRREKASEGLDAQGKPRTRSPALPGGRSPGPCRALEGGQAEVRLRRNASSAGRLQGLAGGAPGQKECRPFEVYLPSGKMRPRSLEVTISTGEKAPANPEILLTLEEKTAANLDSATEPRARPTPDGGASADLKEGPGNPEHSVTGRPPDTAASPRCHAQEGDPVDGTCVRDSCSFPEAVSGHPQASGSRSPGCPRCQDSHERKSPGSLSPQPLPQCKRHLKQVQLLFCEDHDEPICLICSLSQEHQGHRVRPIEEVALEHKKIQKQLEHLKKLRKSGEEQRSYGEEKAVSFLKQTEALKQRVQRKLEQVYYFLEQQEHFFVASLEDVGQMVGQIRKAYDTRVSQDIALLDALIGELEAKECQSEWELLQDIGDILHRAKTVPVPEKWTTPQEIKQKIQLLHQKSEFVEKSTKYFSETLRSEMEMFNVPELIGAQAHAVNVILDAETAYPNLIFSDDLKSVRLGNKWERLPDGPQRFDSCIIVLGSPSFLSGRRYWEVEVGDKTAWILGACKTSISRKGNMTLSPENGYWVVIMMKENEYQASSVPPTRLLIKEPPKRVGIFVDYRVGSISFYNVTARSHIYTFASCSFSGPLQPIFSPGTRDGGKNTAPLTICPVGGQGPD

Target ORF information:

RefSeq Version	XM_017023236.1
Organism	Homo sapiens(human)
Definition	Homo sapiens Mediterranean fever (MEFV), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017023236.1

ORF Insert Sequence:

ATGGCTAAGA CCCCTAGTGA CCATCTGCTG TCCACCCTGG AGGAGCTGGT GCCCTATGAC 
TTCGAGAAGT TCAAGTTCAA GCTGCAGAAC ACCAGTGTGC AGAAGGAGCA CTCCAGGATC 
CCCCGGAGCC AGATCCAGAG AGCCAGGCCG GTGAAGATGG CCACTCTGCT GGTCACCTAC 
TATGGGGAAG AGTACGCCGT GCAGCTCACC CTGCAGGTCC TGCGGGCCAT CAACCAGCGC 
CTGCTGGCCG AGGAGCTCCA CAGGGCAGCC ATTCAGGAAT ATTCCACACA AGAAAACGGC 
ACAGATGATT CCGCAGCGTC CAGCTCCCTG GGGGAGAACA AGCCCAGGAG CCTGAAGACT 
CCAGACCACC CCGAGGGGAA CGAGGGGAAC GGCCCTCGGC CGTACGGGGG CGGAGCTGCC 
AGCCTGCGGT GCAGCCAGCC CGAGGCCGGG AGGGGGCTGT CGAGGAAGCC CCTGAGCAAA 
CGCAGAGAGA AGGCCTCGGA GGGCCTGGAC GCGCAGGGCA AGCCTCGGAC CCGGAGCCCG 
GCCCTGCCGG GCGGGAGAAG CCCCGGCCCC TGCAGGGCGC TAGAGGGGGG CCAGGCCGAG 
GTCCGGCTGC GCAGAAACGC CAGCTCCGCG GGGAGGCTGC AGGGGCTGGC GGGGGGCGCC 
CCGGGGCAGA AGGAGTGCAG GCCCTTCGAA GTGTACCTGC CCTCGGGAAA GATGCGACCT 
AGAAGCCTTG AGGTCACCAT TTCTACAGGG GAGAAGGCGC CCGCAAATCC AGAAATTCTC 
CTGACTCTAG AGGAAAAGAC AGCTGCGAAT CTGGACTCGG CAACAGAACC CCGGGCAAGG 
CCCACTCCGG ATGGAGGGGC ATCTGCGGAC CTGAAGGAAG GCCCTGGAAA TCCAGAACAT 
TCGGTCACCG GAAGGCCACC AGACACGGCT GCGAGTCCCC GCTGCCACGC CCAGGAAGGA 
GACCCAGTTG ACGGTACCTG TGTGCGTGAT TCCTGCAGCT TCCCCGAGGC AGTTTCTGGG 
CACCCCCAGG CCTCAGGCAG CCGCTCACCT GGCTGCCCCC GGTGCCAGGA CTCCCATGAA 
AGGAAGAGCC CGGGAAGCCT AAGCCCCCAG CCCCTGCCAC AGTGTAAGCG CCACCTGAAG 
CAGGTCCAGC TGCTCTTCTG TGAGGATCAC GATGAGCCCA TCTGCCTCAT CTGCAGTCTG 
AGTCAGGAGC ACCAAGGCCA CCGGGTGCGC CCCATTGAGG AGGTCGCCCT GGAACACAAG 
AAAATTCAGA AGCAGCTGGA GCATCTGAAG AAGCTGAGAA AATCAGGGGA GGAGCAGCGA 
TCCTATGGGG AGGAGAAGGC AGTGAGCTTT CTGAAACAAA CTGAAGCGCT GAAGCAGCGG 
GTGCAGAGGA AGCTGGAGCA GGTGTACTAC TTCCTGGAGC AGCAAGAGCA TTTCTTTGTG 
GCCTCACTGG AGGACGTGGG CCAGATGGTT GGGCAGATCA GGAAGGCATA TGACACCCGC 
GTATCCCAGG ACATCGCCCT GCTCGATGCG CTGATTGGGG AACTGGAGGC CAAGGAGTGC 
CAGTCAGAAT GGGAACTTCT GCAGGACATT GGAGACATCT TGCACAGGGC TAAGACAGTG 
CCTGTCCCTG AAAAGTGGAC CACTCCTCAA GAGATAAAAC AAAAGATCCA ACTCCTCCAC 
CAGAAGTCAG AGTTTGTGGA GAAGAGCACA AAGTACTTCT CAGAAACCCT GCGTTCAGAA 
ATGGAAATGT TCAATGTTCC GGAGCTGATT GGCGCTCAGG CACATGCTGT TAATGTGATT 
CTGGATGCAG AAACCGCTTA CCCCAACCTC ATCTTCTCTG ATGATCTGAA GAGTGTTAGA 
CTTGGAAACA AGTGGGAGAG GCTGCCTGAT GGCCCGCAAA GATTTGACAG CTGTATCATT 
GTTCTGGGCT CTCCGAGTTT CCTCTCTGGC CGCCGTTACT GGGAGGTGGA GGTTGGAGAC 
AAGACAGCAT GGATCCTGGG AGCCTGCAAG ACATCCATAA GCAGGAAAGG GAACATGACT 
CTGTCGCCAG AGAATGGCTA CTGGGTGGTG ATAATGATGA AGGAAAATGA GTACCAGGCG 
TCCAGCGTTC CCCCGACCCG CCTGCTAATA AAGGAGCCTC CCAAGCGTGT GGGCATCTTC 
GTGGACTACA GAGTTGGAAG CATCTCCTTT TACAATGTGA CAGCCAGATC CCACATCTAT 
ACATTCGCCA GCTGCTCTTT CTCTGGGCCC CTTCAACCTA TCTTCAGCCC TGGGACACGT 
GATGGAGGGA AGAACACAGC TCCTCTGACT ATCTGTCCAG TGGGTGGTCA GGGGCCTGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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MEFV cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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