PIM3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PIM3
Entrez Gene ID	415116
Full Name	Pim-3 proto-oncogene, serine/threonine kinase
Synonyms	pim-3
General protein information	Preferred Names Pim-3 proto-oncogene, serine/threonine kinase Names serine/threonine-protein kinase pim-3 pim-3 oncogene serine/threonine kinase Pim-3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q13.33
Summary	The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012].
Disorder MIM:	610580

mRNA and Protein(s)

mRNA	Protein	Name
NM_001001852.4	NP_001001852.2	serine/threonine-protein kinase pim-3
NM_001001852.3	NP_001001852.2	serine/threonine-protein kinase pim-3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	LOC100497723	XP_002932230.1
Rattus norvegicus (Norway rat)	Pim3	NP_072124.1
Gallus gallus (chicken)	PIM3	XP_003640390.1
	PIM3	XP_001116171.2
Mus musculus (house mouse)	Pim3	NP_663453.1
Homo sapiens (human)	PIM3	NP_001001852.2
Bos taurus (cattle)	PIM3	XP_002688056.3

Gene Ontology
Bibliography

Related articles in PubMed

mTORC1 suppresses PIM3 expression via miR-33 encoded by the SREBP loci.
Kelsey I, Zbinden M, Byles V, Torrence M, Manning BD
Scientific reports7(1)16112(2017 Nov)

PIM kinases 1, 2 and 3 in intracellular LIF signaling, proliferation and apoptosis in trophoblastic cells.
Mary Photini S, Chaiwangyen W, Weber M, Al-Kawlani B, Favaro RR, Jeschke U, Schleussner E, Morales-Prieto DM, Markert UR
Experimental cell research359(1)275-283(2017 10)

Expression of PIM kinases in Reed-Sternberg cells fosters immune privilege and tumor cell survival in Hodgkin lymphoma.
Szyd?owski M, Prochorec-Sobieszek M, Szumera-Cie?kiewicz A, Derezi?ska E, Hoser G, Wasilewska D, Szyma?ska-Giemza O, Jab?o?ska E, Bia?opiotrowicz E, Sewastianik T, Polak A, Czardybon W, Ga??zowski M, Windak R, Zaucha JM, Warzocha K, Brz?zka K, Juszczy?ski P
Blood130(12)1418-1429(2017 09)

Up-regulation of Pim-3 in Chronic Obstructive Pulmonary Disease (COPD) patients and its potential therapeutic role in COPD rat modeling.
Yang C, Li L, Guo J, Zhang W, Zhu W, Rao X, Huang W
Pathology, research and practice213(4)322-326(2017 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These results demonstrate the involvement of PIM kinases in LIF-induced regulation in different trophoblastic cell lines which may indicate similar functions in primary cells.
Title: PIM kinases 1, 2 and 3 in intracellular LIF signaling, proliferation and apoptosis in trophoblastic cells.

PIM kinases in classical Hodgkin lymphoma exhibit pleiotropic effects, orchestrating tumor immune escape and supporting Reed-Sternberg cell survival.
Title: Expression of PIM kinases in Reed-Sternberg cells fosters immune privilege and tumor cell survival in Hodgkin lymphoma.

Our study suggests that up-regulation of Pim-3 successfully accelerated Chronic Obstructive Pulmonary Disease development, and aggravated lung damage
Title: Up-regulation of Pim-3 in Chronic Obstructive Pulmonary Disease (COPD) patients and its potential therapeutic role in COPD rat modeling.

The expression status of Pim-3 mRNA was significantly associated with pathological parameters such as pre-surgery prostate specific antigen, Gleason score, pathological stage, and lymphoid metastasis.
Title: Pim-3 is a Critical Risk Factor in Development and Prognosis of Prostate Cancer.

High PIM3 expression is associated with osteosarcoma.
Title: Expression and function of PIM kinases in osteosarcoma.

The following PIM3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PIM3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu28503	NM_001001852.4 Latest version!	Homo sapiens Pim-3 proto-oncogene, serine/threonine kinase (PIM3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu28503	NM_001001852.3	Homo sapiens Pim-3 proto-oncogene, serine/threonine kinase (PIM3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PIM3 ( NM_001001852.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001852.2 Homo sapiens Pim-3 proto-oncogene, serine/threonine kinase (PIM3), mRNA.

Price & Availability↑

CloneID	OHu28503
Clone ID Related Accession (Same CDS sequence)	NM_001001852.4 , NM_001001852.3
Accession Version	NM_001001852.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase pim-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB482257.1, AB114795.1, CT000425.1 and CR536608.3. On Nov 22, 2018 this sequence version replaced NM_001001852.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB114795.1, SRR1163655.75044.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000360612.5/ ENSP00000353824.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGCTCT CCAAGTTCGG CTCCCTGGCG CACCTCTGCG GGCCCGGCGG CGTGGACCAC 
CTCCCGGTGA AGATCCTGCA GCCAGCCAAG GCGGACAAGG AGAGCTTCGA GAAGGCGTAC 
CAGGTGGGCG CCGTGCTGGG TAGCGGCGGC TTCGGCACGG TCTACGCGGG TAGCCGCATC 
GCCGACGGGC TCCCGGTGGC TGTGAAGCAC GTGGTGAAGG AGCGGGTGAC CGAGTGGGGC 
AGCCTGGGCG GCGCGACCGT GCCCCTGGAG GTGGTGCTGC TGCGCAAGGT GGGCGCGGCG 
GGCGGCGCGC GCGGCGTCAT CCGCCTGCTG GACTGGTTCG AGCGGCCCGA CGGCTTCCTG 
CTGGTGCTGG AGCGGCCCGA GCCGGCGCAG GACCTCTTCG ACTTTATCAC GGAGCGCGGC 
GCCCTGGACG AGCCGCTGGC GCGCCGCTTC TTCGCGCAGG TGCTGGCCGC CGTGCGCCAC 
TGCCACAGCT GCGGGGTCGT GCACCGCGAC ATTAAGGACG AAAATCTGCT TGTGGACCTG 
CGCTCCGGAG AGCTCAAGCT CATCGACTTC GGTTCGGGTG CGCTGCTCAA GGACACGGTC 
TACACCGACT TCGACGGCAC CCGAGTGTAC AGCCCCCCGG AGTGGATCCG CTACCACCGC 
TACCACGGGC GCTCGGCCAC CGTGTGGTCG CTGGGCGTGC TTCTCTACGA TATGGTGTGT 
GGGGACATCC CCTTCGAGCA GGACGAGGAG ATCCTCCGAG GCCGCCTGCT CTTCCGGAGG 
AGGGTCTCTC CAGAGTGCCA GCAGCTGATC CGGTGGTGCC TGTCCCTGCG GCCCTCAGAG 
CGGCCGTCGC TGGATCAGAT TGCGGCCCAT CCCTGGATGC TGGGGGCTGA CGGGGGCGTC 
CCGGAGAGCT GTGACCTGCG GCTGTGCACC CTCGACCCTG ATGACGTGGC CAGCACCACG 
TCCAGCAGCG AGAGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001852.2
CDS	177..1157
Translation	MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSERPSLDQIAAHPWMLGADGGVPESCDLRLCTLDPDDVASTTSSSESL

Target ORF information:

RefSeq Version	NM_001001852.4
Organism	Homo sapiens(human)
Definition	Homo sapiens Pim-3 proto-oncogene, serine/threonine kinase (PIM3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001852.4

ORF Insert Sequence:

ATGCTGCTCT CCAAGTTCGG CTCCCTGGCG CACCTCTGCG GGCCCGGCGG CGTGGACCAC 
CTCCCGGTGA AGATCCTGCA GCCAGCCAAG GCGGACAAGG AGAGCTTCGA GAAGGCGTAC 
CAGGTGGGCG CCGTGCTGGG TAGCGGCGGC TTCGGCACGG TCTACGCGGG TAGCCGCATC 
GCCGACGGGC TCCCGGTGGC TGTGAAGCAC GTGGTGAAGG AGCGGGTGAC CGAGTGGGGC 
AGCCTGGGCG GCGCGACCGT GCCCCTGGAG GTGGTGCTGC TGCGCAAGGT GGGCGCGGCG 
GGCGGCGCGC GCGGCGTCAT CCGCCTGCTG GACTGGTTCG AGCGGCCCGA CGGCTTCCTG 
CTGGTGCTGG AGCGGCCCGA GCCGGCGCAG GACCTCTTCG ACTTTATCAC GGAGCGCGGC 
GCCCTGGACG AGCCGCTGGC GCGCCGCTTC TTCGCGCAGG TGCTGGCCGC CGTGCGCCAC 
TGCCACAGCT GCGGGGTCGT GCACCGCGAC ATTAAGGACG AAAATCTGCT TGTGGACCTG 
CGCTCCGGAG AGCTCAAGCT CATCGACTTC GGTTCGGGTG CGCTGCTCAA GGACACGGTC 
TACACCGACT TCGACGGCAC CCGAGTGTAC AGCCCCCCGG AGTGGATCCG CTACCACCGC 
TACCACGGGC GCTCGGCCAC CGTGTGGTCG CTGGGCGTGC TTCTCTACGA TATGGTGTGT 
GGGGACATCC CCTTCGAGCA GGACGAGGAG ATCCTCCGAG GCCGCCTGCT CTTCCGGAGG 
AGGGTCTCTC CAGAGTGCCA GCAGCTGATC CGGTGGTGCC TGTCCCTGCG GCCCTCAGAG 
CGGCCGTCGC TGGATCAGAT TGCGGCCCAT CCCTGGATGC TGGGGGCTGA CGGGGGCGTC 
CCGGAGAGCT GTGACCTGCG GCTGTGCACC CTCGACCCTG ATGACGTGGC CAGCACCACG 
TCCAGCAGCG AGAGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PIM3 ( NM_001001852.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001852.2 Homo sapiens Pim-3 proto-oncogene, serine/threonine kinase (PIM3), mRNA.

Price & Availability↑

CloneID	OHu28503
Clone ID Related Accession (Same CDS sequence)	NM_001001852.4 , NM_001001852.3
Accession Version	NM_001001852.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 981bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-23
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase pim-3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB482257.1, AB114795.1, CT000425.1 and CR536608.3. On Mar 6, 2009 this sequence version replaced NM_001001852.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB114795.1, SRR1163657.25045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGCTGCTCT CCAAGTTCGG CTCCCTGGCG CACCTCTGCG GGCCCGGCGG CGTGGACCAC 
CTCCCGGTGA AGATCCTGCA GCCAGCCAAG GCGGACAAGG AGAGCTTCGA GAAGGCGTAC 
CAGGTGGGCG CCGTGCTGGG TAGCGGCGGC TTCGGCACGG TCTACGCGGG TAGCCGCATC 
GCCGACGGGC TCCCGGTGGC TGTGAAGCAC GTGGTGAAGG AGCGGGTGAC CGAGTGGGGC 
AGCCTGGGCG GCGCGACCGT GCCCCTGGAG GTGGTGCTGC TGCGCAAGGT GGGCGCGGCG 
GGCGGCGCGC GCGGCGTCAT CCGCCTGCTG GACTGGTTCG AGCGGCCCGA CGGCTTCCTG 
CTGGTGCTGG AGCGGCCCGA GCCGGCGCAG GACCTCTTCG ACTTTATCAC GGAGCGCGGC 
GCCCTGGACG AGCCGCTGGC GCGCCGCTTC TTCGCGCAGG TGCTGGCCGC CGTGCGCCAC 
TGCCACAGCT GCGGGGTCGT GCACCGCGAC ATTAAGGACG AAAATCTGCT TGTGGACCTG 
CGCTCCGGAG AGCTCAAGCT CATCGACTTC GGTTCGGGTG CGCTGCTCAA GGACACGGTC 
TACACCGACT TCGACGGCAC CCGAGTGTAC AGCCCCCCGG AGTGGATCCG CTACCACCGC 
TACCACGGGC GCTCGGCCAC CGTGTGGTCG CTGGGCGTGC TTCTCTACGA TATGGTGTGT 
GGGGACATCC CCTTCGAGCA GGACGAGGAG ATCCTCCGAG GCCGCCTGCT CTTCCGGAGG 
AGGGTCTCTC CAGAGTGCCA GCAGCTGATC CGGTGGTGCC TGTCCCTGCG GCCCTCAGAG 
CGGCCGTCGC TGGATCAGAT TGCGGCCCAT CCCTGGATGC TGGGGGCTGA CGGGGGCGTC 
CCGGAGAGCT GTGACCTGCG GCTGTGCACC CTCGACCCTG ATGACGTGGC CAGCACCACG 
TCCAGCAGCG AGAGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001852.2
CDS	454..1434
Translation	MLLSKFGSLAHLCGPGGVDHLPVKILQPAKADKESFEKAYQVGAVLGSGGFGTVYAGSRIADGLPVAVKHVVKERVTEWGSLGGATVPLEVVLLRKVGAAGGARGVIRLLDWFERPDGFLLVLERPEPAQDLFDFITERGALDEPLARRFFAQVLAAVRHCHSCGVVHRDIKDENLLVDLRSGELKLIDFGSGALLKDTVYTDFDGTRVYSPPEWIRYHRYHGRSATVWSLGVLLYDMVCGDIPFEQDEEILRGRLLFRRRVSPECQQLIRWCLSLRPSERPSLDQIAAHPWMLGADGGVPESCDLRLCTLDPDDVASTTSSSESL

Target ORF information:

RefSeq Version	NM_001001852.3
Organism	Homo sapiens(human)
Definition	Homo sapiens Pim-3 proto-oncogene, serine/threonine kinase (PIM3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001852.3

ORF Insert Sequence:

ATGCTGCTCT CCAAGTTCGG CTCCCTGGCG CACCTCTGCG GGCCCGGCGG CGTGGACCAC 
CTCCCGGTGA AGATCCTGCA GCCAGCCAAG GCGGACAAGG AGAGCTTCGA GAAGGCGTAC 
CAGGTGGGCG CCGTGCTGGG TAGCGGCGGC TTCGGCACGG TCTACGCGGG TAGCCGCATC 
GCCGACGGGC TCCCGGTGGC TGTGAAGCAC GTGGTGAAGG AGCGGGTGAC CGAGTGGGGC 
AGCCTGGGCG GCGCGACCGT GCCCCTGGAG GTGGTGCTGC TGCGCAAGGT GGGCGCGGCG 
GGCGGCGCGC GCGGCGTCAT CCGCCTGCTG GACTGGTTCG AGCGGCCCGA CGGCTTCCTG 
CTGGTGCTGG AGCGGCCCGA GCCGGCGCAG GACCTCTTCG ACTTTATCAC GGAGCGCGGC 
GCCCTGGACG AGCCGCTGGC GCGCCGCTTC TTCGCGCAGG TGCTGGCCGC CGTGCGCCAC 
TGCCACAGCT GCGGGGTCGT GCACCGCGAC ATTAAGGACG AAAATCTGCT TGTGGACCTG 
CGCTCCGGAG AGCTCAAGCT CATCGACTTC GGTTCGGGTG CGCTGCTCAA GGACACGGTC 
TACACCGACT TCGACGGCAC CCGAGTGTAC AGCCCCCCGG AGTGGATCCG CTACCACCGC 
TACCACGGGC GCTCGGCCAC CGTGTGGTCG CTGGGCGTGC TTCTCTACGA TATGGTGTGT 
GGGGACATCC CCTTCGAGCA GGACGAGGAG ATCCTCCGAG GCCGCCTGCT CTTCCGGAGG 
AGGGTCTCTC CAGAGTGCCA GCAGCTGATC CGGTGGTGCC TGTCCCTGCG GCCCTCAGAG 
CGGCCGTCGC TGGATCAGAT TGCGGCCCAT CCCTGGATGC TGGGGGCTGA CGGGGGCGTC 
CCGGAGAGCT GTGACCTGCG GCTGTGCACC CTCGACCCTG ATGACGTGGC CAGCACCACG 
TCCAGCAGCG AGAGCTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

mTORC1 suppresses PIM3 expression via miR-33 encoded by the SREBP loci.
Scientific reports7(1)16112(2017 Nov)
Kelsey I,Zbinden M,Byles V,Torrence M,Manning BD

PIM kinases 1, 2 and 3 in intracellular LIF signaling, proliferation and apoptosis in trophoblastic cells.
Experimental cell research359(1)275-283(2017 10)
Mary Photini S,Chaiwangyen W,Weber M,Al-Kawlani B,Favaro RR,Jeschke U,Schleussner E,Morales-Prieto DM,Markert UR

Expression of PIM kinases in Reed-Sternberg cells fosters immune privilege and tumor cell survival in Hodgkin lymphoma.
Blood130(12)1418-1429(2017 09)
Szyd?owski M,Prochorec-Sobieszek M,Szumera-Cie?kiewicz A,Derezi?ska E,Hoser G,Wasilewska D,Szyma?ska-Giemza O,Jab?o?ska E,Bia?opiotrowicz E,Sewastianik T,Polak A,Czardybon W,Ga??zowski M,Windak R,Zaucha JM,Warzocha K,Brz?zka K,Juszczy?ski P

Up-regulation of Pim-3 in Chronic Obstructive Pulmonary Disease (COPD) patients and its potential therapeutic role in COPD rat modeling.
Pathology, research and practice213(4)322-326(2017 Apr)
Yang C,Li L,Guo J,Zhang W,Zhu W,Rao X,Huang W

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PIM3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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