MAN2B1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAN2B1
Entrez Gene ID	4125
Full Name	mannosidase alpha class 2B member 1
Synonyms	LAMAN,MANB
General protein information	Preferred Names mannosidase alpha class 2B member 1 Names lysosomal alpha-mannosidase lysosomal acid alpha-mannosidase mannosidase alpha-B mannosidase, alpha B, lysosomal
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.13
Summary	This gene encodes an enzyme that hydrolyzes terminal, non-reducing alpha-D-mannose residues in alpha-D-mannosides. Its activity is necessary for the catabolism of N-linked carbohydrates released during glycoprotein turnover and it is member of family 38 of glycosyl hydrolases. The full length protein is processed in two steps. First, a 49 aa leader sequence is cleaved off and the remainder of the protein is processed into 3 peptides of 70 kDa, 42 kDa (D) and 13/15 kDa (E). Next, the 70 kDa peptide is further processed into three peptides (A, B and C). The A, B and C peptides are disulfide-linked. Defects in this gene have been associated with lysosomal alpha-mannosidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2010].
Disorder MIM:	609458
Disorder Html:	Mannosidosis, alpha-, types I and II, 248500 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000528.3	NP_000519.2	lysosomal alpha-mannosidase isoform 1 precursor
XM_024451518.1	XP_024307286.1	lysosomal alpha-mannosidase isoform X2
XM_017026818.1	XP_016882307.1	lysosomal alpha-mannosidase isoform X2
NM_001173498.1	NP_001166969.1	lysosomal alpha-mannosidase isoform 2 precursor
XM_005259913.2	XP_005259970.1	lysosomal alpha-mannosidase isoform X1
NM_000528.4	NP_000519.2	lysosomal alpha-mannosidase isoform 1 precursor
XM_005259913.1	XP_005259970.1	lysosomal alpha-mannosidase isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAN2B1	XP_003316198.2
Arabidopsis thaliana (thale cress)	AT5G13980	NP_196902.2
Bos taurus (cattle)	MAN2B1	NP_776986.2
Rattus norvegicus (Norway rat)	Man2b1	NP_955436.1
Mus musculus (house mouse)	Man2b1	NP_034894.2
Drosophila melanogaster (fruit fly)	LM408	NP_609408.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008584	XP_557731.3
Xenopus tropicalis (tropical clawed frog)	man2b1	NP_001072469.1
Homo sapiens (human)	MAN2B1	NP_000519.2
	LOC716173	XP_002801133.1
Canis lupus familiaris (dog)	MAN2B1	XP_005632890.1
Danio rerio (zebrafish)	man2b1	NP_001014354.1
Caenorhabditis elegans (roundworm)	aman-1	NP_508811.1
Oryza sativa (rice)	Os11g0525600	NP_001067993.1

Gene Ontology
Bibliography

Related articles in PubMed

Lysosomal storage disease in the brain: mutations of the ?-mannosidase gene identified in autosomal dominant nystagmus.
Yu P, Cui Y, Cai W, Wu H, Xiao X, Shao Q, Ma L, Guo S, Wu N, Jin ZB, Wang Y, Cai T, Sun ZS, Qu J
Genetics in medicine : official journal of the American College of Medical Genetics17(12)971-9(2015 Dec)

Alpha-mannosidosis: correlation between phenotype, genotype and mutant MAN2B1 subcellular localisation.
Borgwardt L, Stensland HM, Olsen KJ, Wibrand F, Klenow HB, Beck M, Amraoui Y, Arash L, Fogh J, Nilssen ?, Dali CI, Lund AM
Orphanet journal of rare diseases1070(2015 Jun)

Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum.
Thaysen-Andersen M, Venkatakrishnan V, Loke I, Laurini C, Diestel S, Parker BL, Packer NH
The Journal of biological chemistry290(14)8789-802(2015 Apr)

Molecular diagnosis of a Chinese pedigree with ?-mannosidosis and identification of a novel missense mutation.
Wu X, Pan J, Guo Y, Guo C, Jiang W, Li R, Tang J, Ai Y
Journal of pediatric endocrinology & metabolism : JPEM27(5-6)491-5(2014 May)

Genome-wide study of percent emphysema on computed tomography in the general population. The Multi-Ethnic Study of Atherosclerosis Lung/SNP Health Association Resource Study.
Manichaikul A, Hoffman EA, Smolonska J, Gao W, Cho MH, Baumhauer H, Budoff M, Austin JH, Washko GR, Carr JJ, Kaufman JD, Pottinger T, Powell CA, Wijmenga C, Zanen P, Groen HJ, Postma DS, Wanner A, Rouhani FN, Brantly ML, Powell R, Smith BM, Rabinowitz D, Raffel LJ, Hinckley Stukovsky KD, Crapo JD, Beaty TH, Hokanson JE, Silverman EK, Dupuis J, O'Connor GT, Boezen HM, Rich SS, Barr RG
American journal of respiratory and critical care medicine189(4)408-18(2014 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel heterozygous mutation (c.2013G>A; p.R638H) of MANBA was identified in patients with autosomal-dominant nystagmus. An additional mutation (c.2346T>A; p.L749H) in MANBA was found by screening patients with sporadic nystagmus.
Title: Lysosomal storage disease in the brain: mutations of the ?-mannosidase gene identified in autosomal dominant nystagmus.

Our results indicate a correlation between the MAN2B1 genotypes and the cognitive function, upper limb coordination, balance, FVC% and the storage of oligosaccharides in CSF.
Title: Alpha-mannosidosis: correlation between phenotype, genotype and mutant MAN2B1 subcellular localisation.

The results showed that the alpha-mannosidosis patient has compound heterozygous mutations in the MAN2B1 gene
Title: Molecular diagnosis of a Chinese pedigree with ?-mannosidosis and identification of a novel missense mutation.

A single nucleotide polymorphism in MAN2B, rs228614, is associated with risk for multiple sclerosis.
Title: MANBA, CXCR5, SOX8, RPS6KB1 and ZBTB46 are genetic risk loci for multiple sclerosis.

MAN2B1 is targeted to the vacuole without passing through the Golgi complex.
Title: Traffic of human ?-mannosidase in plant cells suggests the presence of a new endoplasmic reticulum-to-vacuole pathway without involving the Golgi complex.

The following MAN2B1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAN2B1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18492	NM_000528.4 Latest version!	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu58847	XM_024451518.1 Latest version!	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu58847	XM_017026818.1 Latest version!	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu16780	NM_001173498.1 Latest version!	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu51603	XM_005259913.2 Latest version!	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu18492	NM_000528.3	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu51603	XM_005259913.1	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAN2B1 ( NM_000528.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000519.2 Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18492
Clone ID Related Accession (Same CDS sequence)	NM_000528.3 , NM_000528.4
Accession Version	NM_000528.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	lysosomal alpha-mannosidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB237901.1, BC000736.2 and BM982008.1. This sequence is a reference standard in the RefSeqGene project. On May 9, 2008 this sequence version replaced NM_000528.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291572.1, SRR1803612.260285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGG CAAAAGGAAG CAGTGTCCAT GTTCTCTACT CCACCCCCGC TTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCAGTGAAAC ATGACGACTT CTTCCCTTAC 
GCGGATGGCC CCCACCAGTT CTGGACCGGT TACTTTTCCA GTCGGCCGGC CCTCAAACGC 
TACGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGTGGGC 
CTGGCGGCCA ACGTGGGACC CTATGGCTCC GGAGACAGTG CACCCCTCAA TGAGGCGATG 
GCTGTGCTCC AGCATCACGA CGCCGTCAGC GGCACCTCCC GCCAGCACGT GGCCAACGAC 
TACGCGCGCC AGCTTGCGGC AGGCTGGGGG CCTTGCGAGG TTCTTCTGAG CAACGCGCTG 
GCGCGGCTCA GAGGCTTCAA AGATCACTTC ACCTTTTGCC AACAGCTAAA CATCAGCATC 
TGCCCGCTCA GCCAGACGGC GGCGCGCTTC CAGGTCATCG TTTATAATCC CCTGGGGCGG 
AAGGTGAATT GGATGGTACG GCTGCCGGTC AGCGAAGGCG TTTTCGTTGT GAAGGACCCC 
AATGGCAGGA CAGTGCCCAG CGATGTGGTA ATATTTCCCA GCTCAGACAG CCAGGCGCAC 
CCTCCGGAGC TGCTGTTCTC AGCCTCACTG CCCGCCCTGG GCTTCAGCAC CTATTCAGTA 
GCCCAGGTGC CTCGCTGGAA GCCCCAGGCC CGCGCACCAC AGCCCATCCC CAGAAGATCC 
TGGTCCCCTG CTTTAACCAT CGAAAATGAG CACATCCGGG CAACGTTTGA TCCTGACACA 
GGGCTGTTGA TGGAGATTAT GAACATGAAT CAGCAACTCC TGCTGCCTGT TCGCCAGACC 
TTCTTCTGGT ACAACGCCAG TATAGGTGAC AACGAAAGTG ACCAGGCCTC AGGTGCCTAC 
ATCTTCAGAC CCAACCAACA GAAACCGCTG CCTGTGAGCC GCTGGGCTCA GATCCACCTG 
GTGAAGACAC CCTTGGTGCA GGAGGTGCAC CAGAACTTCT CAGCTTGGTG TTCCCAGGTG 
GTTCGCCTGT ACCCAGGACA GCGGCACCTG GAGCTAGAGT GGTCGGTGGG GCCGATACCT 
GTGGGCGACA CCTGGGGGAA GGAGGTCATC AGCCGTTTTG ACACACCGCT GGAGACAAAG 
GGACGCTTCT ACACAGACAG CAATGGCCGG GAGATCCTGG AGAGGAGGCG GGATTATCGA 
CCCACCTGGA AACTGAACCA GACGGAGCCC GTGGCAGGAA ACTACTATCC AGTCAACACC 
CGGATTTACA TCACGGATGG AAACATGCAG CTGACTGTGC TGACTGACCG CTCCCAGGGG 
GGCAGCAGCC TGAGAGATGG CTCGCTGGAG CTCATGGTGC ACCGAAGGCT GCTGAAGGAC 
GATGGACGCG GAGTATCGGA GCCACTAATG GAGAACGGGT CGGGGGCGTG GGTGCGAGGG 
CGCCACCTGG TGCTGCTGGA CACAGCCCAG GCTGCAGCCG CCGGACACCG GCTCCTGGCG 
GAGCAGGAGG TCCTGGCCCC TCAGGTGGTG CTGGCCCCGG GTGGCGGCGC CGCCTACAAT 
CTCGGGGCTC CTCCGCGCAC GCAGTTCTCA GGGCTGCGCA GGGACCTGCC GCCCTCGGTG 
CACCTGCTCA CGCTGGCCAG CTGGGGCCCC GAAATGGTGC TGCTGCGCTT GGAGCACCAG 
TTTGCCGTAG GAGAGGATTC CGGACGTAAC CTGAGCGCCC CCGTTACCTT GAACTTGAGG 
GACCTGTTCT CCACCTTCAC CATCACCCGC CTGCAGGAGA CCACGCTGGT GGCCAACCAG 
CTCCGCGAGG CAGCCTCCAG GCTCAAGTGG ACAACAAACA CAGGCCCCAC ACCCCACCAA 
ACTCCGTACC AGCTGGACCC GGCCAACATC ACGCTGGAAC CCATGGAAAT CCGCACTTTC 
CTGGCCTCAG TTCAATGGAA GGAGGTGGAT GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000519.2
CDS	77..3112
Translation	MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Target ORF information:

RefSeq Version	NM_000528.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000528.3

ORF Insert Sequence:

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGG CAAAAGGAAG CAGTGTCCAT GTTCTCTACT CCACCCCCGC TTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCAGTGAAAC ATGACGACTT CTTCCCTTAC 
GCGGATGGCC CCCACCAGTT CTGGACCGGT TACTTTTCCA GTCGGCCGGC CCTCAAACGC 
TACGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGTGGGC 
CTGGCGGCCA ACGTGGGACC CTATGGCTCC GGAGACAGTG CACCCCTCAA TGAGGCGATG 
GCTGTGCTCC AGCATCACGA CGCCGTCAGC GGCACCTCCC GCCAGCACGT GGCCAACGAC 
TACGCGCGCC AGCTTGCGGC AGGCTGGGGG CCTTGCGAGG TTCTTCTGAG CAACGCGCTG 
GCGCGGCTCA GAGGCTTCAA AGATCACTTC ACCTTTTGCC AACAGCTAAA CATCAGCATC 
TGCCCGCTCA GCCAGACGGC GGCGCGCTTC CAGGTCATCG TTTATAATCC CCTGGGGCGG 
AAGGTGAATT GGATGGTACG GCTGCCGGTC AGCGAAGGCG TTTTCGTTGT GAAGGACCCC 
AATGGCAGGA CAGTGCCCAG CGATGTGGTA ATATTTCCCA GCTCAGACAG CCAGGCGCAC 
CCTCCGGAGC TGCTGTTCTC AGCCTCACTG CCCGCCCTGG GCTTCAGCAC CTATTCAGTA 
GCCCAGGTGC CTCGCTGGAA GCCCCAGGCC CGCGCACCAC AGCCCATCCC CAGAAGATCC 
TGGTCCCCTG CTTTAACCAT CGAAAATGAG CACATCCGGG CAACGTTTGA TCCTGACACA 
GGGCTGTTGA TGGAGATTAT GAACATGAAT CAGCAACTCC TGCTGCCTGT TCGCCAGACC 
TTCTTCTGGT ACAACGCCAG TATAGGTGAC AACGAAAGTG ACCAGGCCTC AGGTGCCTAC 
ATCTTCAGAC CCAACCAACA GAAACCGCTG CCTGTGAGCC GCTGGGCTCA GATCCACCTG 
GTGAAGACAC CCTTGGTGCA GGAGGTGCAC CAGAACTTCT CAGCTTGGTG TTCCCAGGTG 
GTTCGCCTGT ACCCAGGACA GCGGCACCTG GAGCTAGAGT GGTCGGTGGG GCCGATACCT 
GTGGGCGACA CCTGGGGGAA GGAGGTCATC AGCCGTTTTG ACACACCGCT GGAGACAAAG 
GGACGCTTCT ACACAGACAG CAATGGCCGG GAGATCCTGG AGAGGAGGCG GGATTATCGA 
CCCACCTGGA AACTGAACCA GACGGAGCCC GTGGCAGGAA ACTACTATCC AGTCAACACC 
CGGATTTACA TCACGGATGG AAACATGCAG CTGACTGTGC TGACTGACCG CTCCCAGGGG 
GGCAGCAGCC TGAGAGATGG CTCGCTGGAG CTCATGGTGC ACCGAAGGCT GCTGAAGGAC 
GATGGACGCG GAGTATCGGA GCCACTAATG GAGAACGGGT CGGGGGCGTG GGTGCGAGGG 
CGCCACCTGG TGCTGCTGGA CACAGCCCAG GCTGCAGCCG CCGGACACCG GCTCCTGGCG 
GAGCAGGAGG TCCTGGCCCC TCAGGTGGTG CTGGCCCCGG GTGGCGGCGC CGCCTACAAT 
CTCGGGGCTC CTCCGCGCAC GCAGTTCTCA GGGCTGCGCA GGGACCTGCC GCCCTCGGTG 
CACCTGCTCA CGCTGGCCAG CTGGGGCCCC GAAATGGTGC TGCTGCGCTT GGAGCACCAG 
TTTGCCGTAG GAGAGGATTC CGGACGTAAC CTGAGCGCCC CCGTTACCTT GAACTTGAGG 
GACCTGTTCT CCACCTTCAC CATCACCCGC CTGCAGGAGA CCACGCTGGT GGCCAACCAG 
CTCCGCGAGG CAGCCTCCAG GCTCAAGTGG ACAACAAACA CAGGCCCCAC ACCCCACCAA 
ACTCCGTACC AGCTGGACCC GGCCAACATC ACGCTGGAAC CCATGGAAAT CCGCACTTTC 
CTGGCCTCAG TTCAATGGAA GGAGGTGGAT GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B1 ( NM_000528.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000519.2 Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18492
Clone ID Related Accession (Same CDS sequence)	NM_000528.3 , NM_000528.4
Accession Version	NM_000528.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	lysosomal alpha-mannosidase isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB237901.1 and BC000736.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_000528.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK291572.1, SRR1803612.260285.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000456935.7/ ENSP00000395473.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGG CAAAAGGAAG CAGTGTCCAT GTTCTCTACT CCACCCCCGC TTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCAGTGAAAC ATGACGACTT CTTCCCTTAC 
GCGGATGGCC CCCACCAGTT CTGGACCGGT TACTTTTCCA GTCGGCCGGC CCTCAAACGC 
TACGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGTGGGC 
CTGGCGGCCA ACGTGGGACC CTATGGCTCC GGAGACAGTG CACCCCTCAA TGAGGCGATG 
GCTGTGCTCC AGCATCACGA CGCCGTCAGC GGCACCTCCC GCCAGCACGT GGCCAACGAC 
TACGCGCGCC AGCTTGCGGC AGGCTGGGGG CCTTGCGAGG TTCTTCTGAG CAACGCGCTG 
GCGCGGCTCA GAGGCTTCAA AGATCACTTC ACCTTTTGCC AACAGCTAAA CATCAGCATC 
TGCCCGCTCA GCCAGACGGC GGCGCGCTTC CAGGTCATCG TTTATAATCC CCTGGGGCGG 
AAGGTGAATT GGATGGTACG GCTGCCGGTC AGCGAAGGCG TTTTCGTTGT GAAGGACCCC 
AATGGCAGGA CAGTGCCCAG CGATGTGGTA ATATTTCCCA GCTCAGACAG CCAGGCGCAC 
CCTCCGGAGC TGCTGTTCTC AGCCTCACTG CCCGCCCTGG GCTTCAGCAC CTATTCAGTA 
GCCCAGGTGC CTCGCTGGAA GCCCCAGGCC CGCGCACCAC AGCCCATCCC CAGAAGATCC 
TGGTCCCCTG CTTTAACCAT CGAAAATGAG CACATCCGGG CAACGTTTGA TCCTGACACA 
GGGCTGTTGA TGGAGATTAT GAACATGAAT CAGCAACTCC TGCTGCCTGT TCGCCAGACC 
TTCTTCTGGT ACAACGCCAG TATAGGTGAC AACGAAAGTG ACCAGGCCTC AGGTGCCTAC 
ATCTTCAGAC CCAACCAACA GAAACCGCTG CCTGTGAGCC GCTGGGCTCA GATCCACCTG 
GTGAAGACAC CCTTGGTGCA GGAGGTGCAC CAGAACTTCT CAGCTTGGTG TTCCCAGGTG 
GTTCGCCTGT ACCCAGGACA GCGGCACCTG GAGCTAGAGT GGTCGGTGGG GCCGATACCT 
GTGGGCGACA CCTGGGGGAA GGAGGTCATC AGCCGTTTTG ACACACCGCT GGAGACAAAG 
GGACGCTTCT ACACAGACAG CAATGGCCGG GAGATCCTGG AGAGGAGGCG GGATTATCGA 
CCCACCTGGA AACTGAACCA GACGGAGCCC GTGGCAGGAA ACTACTATCC AGTCAACACC 
CGGATTTACA TCACGGATGG AAACATGCAG CTGACTGTGC TGACTGACCG CTCCCAGGGG 
GGCAGCAGCC TGAGAGATGG CTCGCTGGAG CTCATGGTGC ACCGAAGGCT GCTGAAGGAC 
GATGGACGCG GAGTATCGGA GCCACTAATG GAGAACGGGT CGGGGGCGTG GGTGCGAGGG 
CGCCACCTGG TGCTGCTGGA CACAGCCCAG GCTGCAGCCG CCGGACACCG GCTCCTGGCG 
GAGCAGGAGG TCCTGGCCCC TCAGGTGGTG CTGGCCCCGG GTGGCGGCGC CGCCTACAAT 
CTCGGGGCTC CTCCGCGCAC GCAGTTCTCA GGGCTGCGCA GGGACCTGCC GCCCTCGGTG 
CACCTGCTCA CGCTGGCCAG CTGGGGCCCC GAAATGGTGC TGCTGCGCTT GGAGCACCAG 
TTTGCCGTAG GAGAGGATTC CGGACGTAAC CTGAGCGCCC CCGTTACCTT GAACTTGAGG 
GACCTGTTCT CCACCTTCAC CATCACCCGC CTGCAGGAGA CCACGCTGGT GGCCAACCAG 
CTCCGCGAGG CAGCCTCCAG GCTCAAGTGG ACAACAAACA CAGGCCCCAC ACCCCACCAA 
ACTCCGTACC AGCTGGACCC GGCCAACATC ACGCTGGAAC CCATGGAAAT CCGCACTTTC 
CTGGCCTCAG TTCAATGGAA GGAGGTGGAT GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000519.2
CDS	42..3077
Translation	MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Target ORF information:

RefSeq Version	NM_000528.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000528.4

ORF Insert Sequence:

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGG CAAAAGGAAG CAGTGTCCAT GTTCTCTACT CCACCCCCGC TTGTTACCTC 
TGGGAGCTGA ACAAGGCCAA CCTCACCTGG TCAGTGAAAC ATGACGACTT CTTCCCTTAC 
GCGGATGGCC CCCACCAGTT CTGGACCGGT TACTTTTCCA GTCGGCCGGC CCTCAAACGC 
TACGAGCGCC TCAGCTACAA CTTCCTGCAG GTGTGCAACC AGCTGGAGGC GCTGGTGGGC 
CTGGCGGCCA ACGTGGGACC CTATGGCTCC GGAGACAGTG CACCCCTCAA TGAGGCGATG 
GCTGTGCTCC AGCATCACGA CGCCGTCAGC GGCACCTCCC GCCAGCACGT GGCCAACGAC 
TACGCGCGCC AGCTTGCGGC AGGCTGGGGG CCTTGCGAGG TTCTTCTGAG CAACGCGCTG 
GCGCGGCTCA GAGGCTTCAA AGATCACTTC ACCTTTTGCC AACAGCTAAA CATCAGCATC 
TGCCCGCTCA GCCAGACGGC GGCGCGCTTC CAGGTCATCG TTTATAATCC CCTGGGGCGG 
AAGGTGAATT GGATGGTACG GCTGCCGGTC AGCGAAGGCG TTTTCGTTGT GAAGGACCCC 
AATGGCAGGA CAGTGCCCAG CGATGTGGTA ATATTTCCCA GCTCAGACAG CCAGGCGCAC 
CCTCCGGAGC TGCTGTTCTC AGCCTCACTG CCCGCCCTGG GCTTCAGCAC CTATTCAGTA 
GCCCAGGTGC CTCGCTGGAA GCCCCAGGCC CGCGCACCAC AGCCCATCCC CAGAAGATCC 
TGGTCCCCTG CTTTAACCAT CGAAAATGAG CACATCCGGG CAACGTTTGA TCCTGACACA 
GGGCTGTTGA TGGAGATTAT GAACATGAAT CAGCAACTCC TGCTGCCTGT TCGCCAGACC 
TTCTTCTGGT ACAACGCCAG TATAGGTGAC AACGAAAGTG ACCAGGCCTC AGGTGCCTAC 
ATCTTCAGAC CCAACCAACA GAAACCGCTG CCTGTGAGCC GCTGGGCTCA GATCCACCTG 
GTGAAGACAC CCTTGGTGCA GGAGGTGCAC CAGAACTTCT CAGCTTGGTG TTCCCAGGTG 
GTTCGCCTGT ACCCAGGACA GCGGCACCTG GAGCTAGAGT GGTCGGTGGG GCCGATACCT 
GTGGGCGACA CCTGGGGGAA GGAGGTCATC AGCCGTTTTG ACACACCGCT GGAGACAAAG 
GGACGCTTCT ACACAGACAG CAATGGCCGG GAGATCCTGG AGAGGAGGCG GGATTATCGA 
CCCACCTGGA AACTGAACCA GACGGAGCCC GTGGCAGGAA ACTACTATCC AGTCAACACC 
CGGATTTACA TCACGGATGG AAACATGCAG CTGACTGTGC TGACTGACCG CTCCCAGGGG 
GGCAGCAGCC TGAGAGATGG CTCGCTGGAG CTCATGGTGC ACCGAAGGCT GCTGAAGGAC 
GATGGACGCG GAGTATCGGA GCCACTAATG GAGAACGGGT CGGGGGCGTG GGTGCGAGGG 
CGCCACCTGG TGCTGCTGGA CACAGCCCAG GCTGCAGCCG CCGGACACCG GCTCCTGGCG 
GAGCAGGAGG TCCTGGCCCC TCAGGTGGTG CTGGCCCCGG GTGGCGGCGC CGCCTACAAT 
CTCGGGGCTC CTCCGCGCAC GCAGTTCTCA GGGCTGCGCA GGGACCTGCC GCCCTCGGTG 
CACCTGCTCA CGCTGGCCAG CTGGGGCCCC GAAATGGTGC TGCTGCGCTT GGAGCACCAG 
TTTGCCGTAG GAGAGGATTC CGGACGTAAC CTGAGCGCCC CCGTTACCTT GAACTTGAGG 
GACCTGTTCT CCACCTTCAC CATCACCCGC CTGCAGGAGA CCACGCTGGT GGCCAACCAG 
CTCCGCGAGG CAGCCTCCAG GCTCAAGTGG ACAACAAACA CAGGCCCCAC ACCCCACCAA 
ACTCCGTACC AGCTGGACCC GGCCAACATC ACGCTGGAAC CCATGGAAAT CCGCACTTTC 
CTGGCCTCAG TTCAATGGAA GGAGGTGGAT GGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B1 ( XM_024451518.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024307286.1 Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.

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CloneID	OHu58847
Clone ID Related Accession (Same CDS sequence)	XM_024451518.1 , XM_017026818.1
Accession Version	XM_024451518.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGT CAGTGAAACA TGACGACTTC TTCCCTTACG CGGATGGCCC CCACCAGTTC 
TGGACCGGTT ACTTTTCCAG TCGGCCGGCC CTCAAACGCT ACGAGCGCCT CAGCTACAAC 
TTCCTGCAGG TGTGCAACCA GCTGGAGGCG CTGGTGGGCC TGGCGGCCAA CGTGGGACCC 
TATGGCTCCG GAGACAGTGC ACCCCTCAAT GAGGCGATGG CTGTGCTCCA GCATCACGAC 
GCCGTCAGCG GCACCTCCCG CCAGCACGTG GCCAACGACT ACGCGCGCCA GCTTGCGGCA 
GGCTGGGGGC CTTGCGAGGT TCTTCTGAGC AACGCGCTGG CGCGGCTCAG AGGCTTCAAA 
GATCACTTCA CCTTTTGCCA ACAGCTAAAC ATCAGCATCT GCCCGCTCAG CCAGACGGCG 
GCGCGCTTCC AGGTCATCGT TTATAATCCC CTGGGGCGGA AGGTGAATTG GATGGTACGG 
CTGCCGGTCA GCGAAGGCGT TTTCGTTGTG AAGGACCCCA ATGGCAGGAC AGTGCCCAGC 
GATGTGGTAA TATTTCCCAG CTCAGACAGC CAGGCGCACC CTCCGGAGCT GCTGTTCTCA 
GCCTCACTGC CCGCCCTGGG CTTCAGCACC TATTCAGTAG CCCAGGTGCC TCGCTGGAAG 
CCCCAGGCCC GCGCACCACA GCCCATCCCC AGAAGATCCT GGTCCCCTGC TTTAACCATC 
GAAAATGAGC ACATCCGGGC AACGTTTGAT CCTGACACAG GGCTGTTGAT GGAGATTATG 
AACATGAATC AGCAACTCCT GCTGCCTGTT CGCCAGACCT TCTTCTGGTA CAACGCCAGT 
ATAGGTGACA ACGAAAGTGA CCAGGCCTCA GGTGCCTACA TCTTCAGACC CAACCAACAG 
AAACCGCTGC CTGTGAGCCG CTGGGCTCAG ATCCACCTGG TGAAGACACC CTTGGTGCAG 
GAGGTGCACC AGAACTTCTC AGCTTGGTGT TCCCAGGTGG TTCGCCTGTA CCCAGGACAG 
CGGCACCTGG AGCTAGAGTG GTCGGTGGGG CCGATACCTG TGGGCGACAC CTGGGGGAAG 
GAGGTCATCA GCCGTTTTGA CACACCGCTG GAGACAAAGG GACGCTTCTA CACAGACAGC 
AATGGCCGGG AGATCCTGGA GAGGAGGCGG GATTATCGAC CCACCTGGAA ACTGAACCAG 
ACGGAGCCCG TGGCAGGAAA CTACTATCCA GTCAACACCC GGATTTACAT CACGGATGGA 
AACATGCAGC TGACTGTGCT GACTGACCGC TCCCAGGGGG GCAGCAGCCT GAGAGATGGC 
TCGCTGGAGC TCATGGTGCA CCGAAGGCTG CTGAAGGACG ATGGACGCGG AGTATCGGAG 
CCACTAATGG AGAACGGGTC GGGGGCGTGG GTGCGAGGGC GCCACCTGGT GCTGCTGGAC 
ACAGCCCAGG CTGCAGCCGC CGGACACCGG CTCCTGGCGG AGCAGGAGGT CCTGGCCCCT 
CAGGTGGTGC TGGCCCCGGG TGGCGGCGCC GCCTACAATC TCGGGGCTCC TCCGCGCACG 
CAGTTCTCAG GGCTGCGCAG GGACCTGCCG CCCTCGGTGC ACCTGCTCAC GCTGGCCAGC 
TGGGGCCCCG AAATGGTGCT GCTGCGCTTG GAGCACCAGT TTGCCGTAGG AGAGGATTCC 
GGACGTAACC TGAGCGCCCC CGTTACCTTG AACTTGAGGG ACCTGTTCTC CACCTTCACC 
ATCACCCGCC TGCAGGAGAC CACGCTGGTG GCCAACCAGC TCCGCGAGGC AGCCTCCAGG 
CTCAAGTGGA CAACAAACAC AGGCCCCACA CCCCACCAAA CTCCGTACCA GCTGGACCCG 
GCCAACATCA CGCTGGAACC CATGGAAATC CGCACTTTCC TGGCCTCAGT TCAATGGAAG 
GAGGTGGATG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024307286.1
CDS	1044..2978
Translation	MRRSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Target ORF information:

RefSeq Version	XM_024451518.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024451518.1

ORF Insert Sequence:

ATGCGCAGGT CAGTGAAACA TGACGACTTC TTCCCTTACG CGGATGGCCC CCACCAGTTC 
TGGACCGGTT ACTTTTCCAG TCGGCCGGCC CTCAAACGCT ACGAGCGCCT CAGCTACAAC 
TTCCTGCAGG TGTGCAACCA GCTGGAGGCG CTGGTGGGCC TGGCGGCCAA CGTGGGACCC 
TATGGCTCCG GAGACAGTGC ACCCCTCAAT GAGGCGATGG CTGTGCTCCA GCATCACGAC 
GCCGTCAGCG GCACCTCCCG CCAGCACGTG GCCAACGACT ACGCGCGCCA GCTTGCGGCA 
GGCTGGGGGC CTTGCGAGGT TCTTCTGAGC AACGCGCTGG CGCGGCTCAG AGGCTTCAAA 
GATCACTTCA CCTTTTGCCA ACAGCTAAAC ATCAGCATCT GCCCGCTCAG CCAGACGGCG 
GCGCGCTTCC AGGTCATCGT TTATAATCCC CTGGGGCGGA AGGTGAATTG GATGGTACGG 
CTGCCGGTCA GCGAAGGCGT TTTCGTTGTG AAGGACCCCA ATGGCAGGAC AGTGCCCAGC 
GATGTGGTAA TATTTCCCAG CTCAGACAGC CAGGCGCACC CTCCGGAGCT GCTGTTCTCA 
GCCTCACTGC CCGCCCTGGG CTTCAGCACC TATTCAGTAG CCCAGGTGCC TCGCTGGAAG 
CCCCAGGCCC GCGCACCACA GCCCATCCCC AGAAGATCCT GGTCCCCTGC TTTAACCATC 
GAAAATGAGC ACATCCGGGC AACGTTTGAT CCTGACACAG GGCTGTTGAT GGAGATTATG 
AACATGAATC AGCAACTCCT GCTGCCTGTT CGCCAGACCT TCTTCTGGTA CAACGCCAGT 
ATAGGTGACA ACGAAAGTGA CCAGGCCTCA GGTGCCTACA TCTTCAGACC CAACCAACAG 
AAACCGCTGC CTGTGAGCCG CTGGGCTCAG ATCCACCTGG TGAAGACACC CTTGGTGCAG 
GAGGTGCACC AGAACTTCTC AGCTTGGTGT TCCCAGGTGG TTCGCCTGTA CCCAGGACAG 
CGGCACCTGG AGCTAGAGTG GTCGGTGGGG CCGATACCTG TGGGCGACAC CTGGGGGAAG 
GAGGTCATCA GCCGTTTTGA CACACCGCTG GAGACAAAGG GACGCTTCTA CACAGACAGC 
AATGGCCGGG AGATCCTGGA GAGGAGGCGG GATTATCGAC CCACCTGGAA ACTGAACCAG 
ACGGAGCCCG TGGCAGGAAA CTACTATCCA GTCAACACCC GGATTTACAT CACGGATGGA 
AACATGCAGC TGACTGTGCT GACTGACCGC TCCCAGGGGG GCAGCAGCCT GAGAGATGGC 
TCGCTGGAGC TCATGGTGCA CCGAAGGCTG CTGAAGGACG ATGGACGCGG AGTATCGGAG 
CCACTAATGG AGAACGGGTC GGGGGCGTGG GTGCGAGGGC GCCACCTGGT GCTGCTGGAC 
ACAGCCCAGG CTGCAGCCGC CGGACACCGG CTCCTGGCGG AGCAGGAGGT CCTGGCCCCT 
CAGGTGGTGC TGGCCCCGGG TGGCGGCGCC GCCTACAATC TCGGGGCTCC TCCGCGCACG 
CAGTTCTCAG GGCTGCGCAG GGACCTGCCG CCCTCGGTGC ACCTGCTCAC GCTGGCCAGC 
TGGGGCCCCG AAATGGTGCT GCTGCGCTTG GAGCACCAGT TTGCCGTAGG AGAGGATTCC 
GGACGTAACC TGAGCGCCCC CGTTACCTTG AACTTGAGGG ACCTGTTCTC CACCTTCACC 
ATCACCCGCC TGCAGGAGAC CACGCTGGTG GCCAACCAGC TCCGCGAGGC AGCCTCCAGG 
CTCAAGTGGA CAACAAACAC AGGCCCCACA CCCCACCAAA CTCCGTACCA GCTGGACCCG 
GCCAACATCA CGCTGGAACC CATGGAAATC CGCACTTTCC TGGCCTCAGT TCAATGGAAG 
GAGGTGGATG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B1 ( XM_017026818.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016882307.1 Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu58847
Clone ID Related Accession (Same CDS sequence)	XM_024451518.1 , XM_017026818.1
Accession Version	XM_017026818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1935bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysosomal alpha-mannosidase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGGT CAGTGAAACA TGACGACTTC TTCCCTTACG CGGATGGCCC CCACCAGTTC 
TGGACCGGTT ACTTTTCCAG TCGGCCGGCC CTCAAACGCT ACGAGCGCCT CAGCTACAAC 
TTCCTGCAGG TGTGCAACCA GCTGGAGGCG CTGGTGGGCC TGGCGGCCAA CGTGGGACCC 
TATGGCTCCG GAGACAGTGC ACCCCTCAAT GAGGCGATGG CTGTGCTCCA GCATCACGAC 
GCCGTCAGCG GCACCTCCCG CCAGCACGTG GCCAACGACT ACGCGCGCCA GCTTGCGGCA 
GGCTGGGGGC CTTGCGAGGT TCTTCTGAGC AACGCGCTGG CGCGGCTCAG AGGCTTCAAA 
GATCACTTCA CCTTTTGCCA ACAGCTAAAC ATCAGCATCT GCCCGCTCAG CCAGACGGCG 
GCGCGCTTCC AGGTCATCGT TTATAATCCC CTGGGGCGGA AGGTGAATTG GATGGTACGG 
CTGCCGGTCA GCGAAGGCGT TTTCGTTGTG AAGGACCCCA ATGGCAGGAC AGTGCCCAGC 
GATGTGGTAA TATTTCCCAG CTCAGACAGC CAGGCGCACC CTCCGGAGCT GCTGTTCTCA 
GCCTCACTGC CCGCCCTGGG CTTCAGCACC TATTCAGTAG CCCAGGTGCC TCGCTGGAAG 
CCCCAGGCCC GCGCACCACA GCCCATCCCC AGAAGATCCT GGTCCCCTGC TTTAACCATC 
GAAAATGAGC ACATCCGGGC AACGTTTGAT CCTGACACAG GGCTGTTGAT GGAGATTATG 
AACATGAATC AGCAACTCCT GCTGCCTGTT CGCCAGACCT TCTTCTGGTA CAACGCCAGT 
ATAGGTGACA ACGAAAGTGA CCAGGCCTCA GGTGCCTACA TCTTCAGACC CAACCAACAG 
AAACCGCTGC CTGTGAGCCG CTGGGCTCAG ATCCACCTGG TGAAGACACC CTTGGTGCAG 
GAGGTGCACC AGAACTTCTC AGCTTGGTGT TCCCAGGTGG TTCGCCTGTA CCCAGGACAG 
CGGCACCTGG AGCTAGAGTG GTCGGTGGGG CCGATACCTG TGGGCGACAC CTGGGGGAAG 
GAGGTCATCA GCCGTTTTGA CACACCGCTG GAGACAAAGG GACGCTTCTA CACAGACAGC 
AATGGCCGGG AGATCCTGGA GAGGAGGCGG GATTATCGAC CCACCTGGAA ACTGAACCAG 
ACGGAGCCCG TGGCAGGAAA CTACTATCCA GTCAACACCC GGATTTACAT CACGGATGGA 
AACATGCAGC TGACTGTGCT GACTGACCGC TCCCAGGGGG GCAGCAGCCT GAGAGATGGC 
TCGCTGGAGC TCATGGTGCA CCGAAGGCTG CTGAAGGACG ATGGACGCGG AGTATCGGAG 
CCACTAATGG AGAACGGGTC GGGGGCGTGG GTGCGAGGGC GCCACCTGGT GCTGCTGGAC 
ACAGCCCAGG CTGCAGCCGC CGGACACCGG CTCCTGGCGG AGCAGGAGGT CCTGGCCCCT 
CAGGTGGTGC TGGCCCCGGG TGGCGGCGCC GCCTACAATC TCGGGGCTCC TCCGCGCACG 
CAGTTCTCAG GGCTGCGCAG GGACCTGCCG CCCTCGGTGC ACCTGCTCAC GCTGGCCAGC 
TGGGGCCCCG AAATGGTGCT GCTGCGCTTG GAGCACCAGT TTGCCGTAGG AGAGGATTCC 
GGACGTAACC TGAGCGCCCC CGTTACCTTG AACTTGAGGG ACCTGTTCTC CACCTTCACC 
ATCACCCGCC TGCAGGAGAC CACGCTGGTG GCCAACCAGC TCCGCGAGGC AGCCTCCAGG 
CTCAAGTGGA CAACAAACAC AGGCCCCACA CCCCACCAAA CTCCGTACCA GCTGGACCCG 
GCCAACATCA CGCTGGAACC CATGGAAATC CGCACTTTCC TGGCCTCAGT TCAATGGAAG 
GAGGTGGATG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016882307.1
CDS	757..2691
Translation	MRRSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Target ORF information:

RefSeq Version	XM_017026818.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017026818.1

ORF Insert Sequence:

ATGCGCAGGT CAGTGAAACA TGACGACTTC TTCCCTTACG CGGATGGCCC CCACCAGTTC 
TGGACCGGTT ACTTTTCCAG TCGGCCGGCC CTCAAACGCT ACGAGCGCCT CAGCTACAAC 
TTCCTGCAGG TGTGCAACCA GCTGGAGGCG CTGGTGGGCC TGGCGGCCAA CGTGGGACCC 
TATGGCTCCG GAGACAGTGC ACCCCTCAAT GAGGCGATGG CTGTGCTCCA GCATCACGAC 
GCCGTCAGCG GCACCTCCCG CCAGCACGTG GCCAACGACT ACGCGCGCCA GCTTGCGGCA 
GGCTGGGGGC CTTGCGAGGT TCTTCTGAGC AACGCGCTGG CGCGGCTCAG AGGCTTCAAA 
GATCACTTCA CCTTTTGCCA ACAGCTAAAC ATCAGCATCT GCCCGCTCAG CCAGACGGCG 
GCGCGCTTCC AGGTCATCGT TTATAATCCC CTGGGGCGGA AGGTGAATTG GATGGTACGG 
CTGCCGGTCA GCGAAGGCGT TTTCGTTGTG AAGGACCCCA ATGGCAGGAC AGTGCCCAGC 
GATGTGGTAA TATTTCCCAG CTCAGACAGC CAGGCGCACC CTCCGGAGCT GCTGTTCTCA 
GCCTCACTGC CCGCCCTGGG CTTCAGCACC TATTCAGTAG CCCAGGTGCC TCGCTGGAAG 
CCCCAGGCCC GCGCACCACA GCCCATCCCC AGAAGATCCT GGTCCCCTGC TTTAACCATC 
GAAAATGAGC ACATCCGGGC AACGTTTGAT CCTGACACAG GGCTGTTGAT GGAGATTATG 
AACATGAATC AGCAACTCCT GCTGCCTGTT CGCCAGACCT TCTTCTGGTA CAACGCCAGT 
ATAGGTGACA ACGAAAGTGA CCAGGCCTCA GGTGCCTACA TCTTCAGACC CAACCAACAG 
AAACCGCTGC CTGTGAGCCG CTGGGCTCAG ATCCACCTGG TGAAGACACC CTTGGTGCAG 
GAGGTGCACC AGAACTTCTC AGCTTGGTGT TCCCAGGTGG TTCGCCTGTA CCCAGGACAG 
CGGCACCTGG AGCTAGAGTG GTCGGTGGGG CCGATACCTG TGGGCGACAC CTGGGGGAAG 
GAGGTCATCA GCCGTTTTGA CACACCGCTG GAGACAAAGG GACGCTTCTA CACAGACAGC 
AATGGCCGGG AGATCCTGGA GAGGAGGCGG GATTATCGAC CCACCTGGAA ACTGAACCAG 
ACGGAGCCCG TGGCAGGAAA CTACTATCCA GTCAACACCC GGATTTACAT CACGGATGGA 
AACATGCAGC TGACTGTGCT GACTGACCGC TCCCAGGGGG GCAGCAGCCT GAGAGATGGC 
TCGCTGGAGC TCATGGTGCA CCGAAGGCTG CTGAAGGACG ATGGACGCGG AGTATCGGAG 
CCACTAATGG AGAACGGGTC GGGGGCGTGG GTGCGAGGGC GCCACCTGGT GCTGCTGGAC 
ACAGCCCAGG CTGCAGCCGC CGGACACCGG CTCCTGGCGG AGCAGGAGGT CCTGGCCCCT 
CAGGTGGTGC TGGCCCCGGG TGGCGGCGCC GCCTACAATC TCGGGGCTCC TCCGCGCACG 
CAGTTCTCAG GGCTGCGCAG GGACCTGCCG CCCTCGGTGC ACCTGCTCAC GCTGGCCAGC 
TGGGGCCCCG AAATGGTGCT GCTGCGCTTG GAGCACCAGT TTGCCGTAGG AGAGGATTCC 
GGACGTAACC TGAGCGCCCC CGTTACCTTG AACTTGAGGG ACCTGTTCTC CACCTTCACC 
ATCACCCGCC TGCAGGAGAC CACGCTGGTG GCCAACCAGC TCCGCGAGGC AGCCTCCAGG 
CTCAAGTGGA CAACAAACAC AGGCCCCACA CCCCACCAAA CTCCGTACCA GCTGGACCCG 
GCCAACATCA CGCTGGAACC CATGGAAATC CGCACTTTCC TGGCCTCAGT TCAATGGAAG 
GAGGTGGATG GTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B1 ( NM_001173498.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166969.1 Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 2, mRNA.

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CloneID	OHu16780
Clone ID Related Accession (Same CDS sequence)	NM_001173498.1
Accession Version	NM_001173498.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3033bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Homo sapiens(human)
Product	lysosomal alpha-mannosidase isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB237901.1, U05572.1, AK291572.1 and BM982008.1. Transcript Variant: This variant (2) lacks a CAG copy at an splice site in the CDS, as compared to variant 1. The resulting isoform (2) lacks an internal Q, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U05572.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGGCAA AAGGAAGCAG TGTCCATGTT CTCTACTCCA CCCCCGCTTG TTACCTCTGG 
GAGCTGAACA AGGCCAACCT CACCTGGTCA GTGAAACATG ACGACTTCTT CCCTTACGCG 
GATGGCCCCC ACCAGTTCTG GACCGGTTAC TTTTCCAGTC GGCCGGCCCT CAAACGCTAC 
GAGCGCCTCA GCTACAACTT CCTGCAGGTG TGCAACCAGC TGGAGGCGCT GGTGGGCCTG 
GCGGCCAACG TGGGACCCTA TGGCTCCGGA GACAGTGCAC CCCTCAATGA GGCGATGGCT 
GTGCTCCAGC ATCACGACGC CGTCAGCGGC ACCTCCCGCC AGCACGTGGC CAACGACTAC 
GCGCGCCAGC TTGCGGCAGG CTGGGGGCCT TGCGAGGTTC TTCTGAGCAA CGCGCTGGCG 
CGGCTCAGAG GCTTCAAAGA TCACTTCACC TTTTGCCAAC AGCTAAACAT CAGCATCTGC 
CCGCTCAGCC AGACGGCGGC GCGCTTCCAG GTCATCGTTT ATAATCCCCT GGGGCGGAAG 
GTGAATTGGA TGGTACGGCT GCCGGTCAGC GAAGGCGTTT TCGTTGTGAA GGACCCCAAT 
GGCAGGACAG TGCCCAGCGA TGTGGTAATA TTTCCCAGCT CAGACAGCCA GGCGCACCCT 
CCGGAGCTGC TGTTCTCAGC CTCACTGCCC GCCCTGGGCT TCAGCACCTA TTCAGTAGCC 
CAGGTGCCTC GCTGGAAGCC CCAGGCCCGC GCACCACAGC CCATCCCCAG AAGATCCTGG 
TCCCCTGCTT TAACCATCGA AAATGAGCAC ATCCGGGCAA CGTTTGATCC TGACACAGGG 
CTGTTGATGG AGATTATGAA CATGAATCAG CAACTCCTGC TGCCTGTTCG CCAGACCTTC 
TTCTGGTACA ACGCCAGTAT AGGTGACAAC GAAAGTGACC AGGCCTCAGG TGCCTACATC 
TTCAGACCCA ACCAACAGAA ACCGCTGCCT GTGAGCCGCT GGGCTCAGAT CCACCTGGTG 
AAGACACCCT TGGTGCAGGA GGTGCACCAG AACTTCTCAG CTTGGTGTTC CCAGGTGGTT 
CGCCTGTACC CAGGACAGCG GCACCTGGAG CTAGAGTGGT CGGTGGGGCC GATACCTGTG 
GGCGACACCT GGGGGAAGGA GGTCATCAGC CGTTTTGACA CACCGCTGGA GACAAAGGGA 
CGCTTCTACA CAGACAGCAA TGGCCGGGAG ATCCTGGAGA GGAGGCGGGA TTATCGACCC 
ACCTGGAAAC TGAACCAGAC GGAGCCCGTG GCAGGAAACT ACTATCCAGT CAACACCCGG 
ATTTACATCA CGGATGGAAA CATGCAGCTG ACTGTGCTGA CTGACCGCTC CCAGGGGGGC 
AGCAGCCTGA GAGATGGCTC GCTGGAGCTC ATGGTGCACC GAAGGCTGCT GAAGGACGAT 
GGACGCGGAG TATCGGAGCC ACTAATGGAG AACGGGTCGG GGGCGTGGGT GCGAGGGCGC 
CACCTGGTGC TGCTGGACAC AGCCCAGGCT GCAGCCGCCG GACACCGGCT CCTGGCGGAG 
CAGGAGGTCC TGGCCCCTCA GGTGGTGCTG GCCCCGGGTG GCGGCGCCGC CTACAATCTC 
GGGGCTCCTC CGCGCACGCA GTTCTCAGGG CTGCGCAGGG ACCTGCCGCC CTCGGTGCAC 
CTGCTCACGC TGGCCAGCTG GGGCCCCGAA ATGGTGCTGC TGCGCTTGGA GCACCAGTTT 
GCCGTAGGAG AGGATTCCGG ACGTAACCTG AGCGCCCCCG TTACCTTGAA CTTGAGGGAC 
CTGTTCTCCA CCTTCACCAT CACCCGCCTG CAGGAGACCA CGCTGGTGGC CAACCAGCTC 
CGCGAGGCAG CCTCCAGGCT CAAGTGGACA ACAAACACAG GCCCCACACC CCACCAAACT 
CCGTACCAGC TGGACCCGGC CAACATCACG CTGGAACCCA TGGAAATCCG CACTTTCCTG 
GCCTCAGTTC AATGGAAGGA GGTGGATGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166969.1
CDS	77..3109
Translation	MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Target ORF information:

RefSeq Version	NM_001173498.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173498.1

ORF Insert Sequence:

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGGCAA AAGGAAGCAG TGTCCATGTT CTCTACTCCA CCCCCGCTTG TTACCTCTGG 
GAGCTGAACA AGGCCAACCT CACCTGGTCA GTGAAACATG ACGACTTCTT CCCTTACGCG 
GATGGCCCCC ACCAGTTCTG GACCGGTTAC TTTTCCAGTC GGCCGGCCCT CAAACGCTAC 
GAGCGCCTCA GCTACAACTT CCTGCAGGTG TGCAACCAGC TGGAGGCGCT GGTGGGCCTG 
GCGGCCAACG TGGGACCCTA TGGCTCCGGA GACAGTGCAC CCCTCAATGA GGCGATGGCT 
GTGCTCCAGC ATCACGACGC CGTCAGCGGC ACCTCCCGCC AGCACGTGGC CAACGACTAC 
GCGCGCCAGC TTGCGGCAGG CTGGGGGCCT TGCGAGGTTC TTCTGAGCAA CGCGCTGGCG 
CGGCTCAGAG GCTTCAAAGA TCACTTCACC TTTTGCCAAC AGCTAAACAT CAGCATCTGC 
CCGCTCAGCC AGACGGCGGC GCGCTTCCAG GTCATCGTTT ATAATCCCCT GGGGCGGAAG 
GTGAATTGGA TGGTACGGCT GCCGGTCAGC GAAGGCGTTT TCGTTGTGAA GGACCCCAAT 
GGCAGGACAG TGCCCAGCGA TGTGGTAATA TTTCCCAGCT CAGACAGCCA GGCGCACCCT 
CCGGAGCTGC TGTTCTCAGC CTCACTGCCC GCCCTGGGCT TCAGCACCTA TTCAGTAGCC 
CAGGTGCCTC GCTGGAAGCC CCAGGCCCGC GCACCACAGC CCATCCCCAG AAGATCCTGG 
TCCCCTGCTT TAACCATCGA AAATGAGCAC ATCCGGGCAA CGTTTGATCC TGACACAGGG 
CTGTTGATGG AGATTATGAA CATGAATCAG CAACTCCTGC TGCCTGTTCG CCAGACCTTC 
TTCTGGTACA ACGCCAGTAT AGGTGACAAC GAAAGTGACC AGGCCTCAGG TGCCTACATC 
TTCAGACCCA ACCAACAGAA ACCGCTGCCT GTGAGCCGCT GGGCTCAGAT CCACCTGGTG 
AAGACACCCT TGGTGCAGGA GGTGCACCAG AACTTCTCAG CTTGGTGTTC CCAGGTGGTT 
CGCCTGTACC CAGGACAGCG GCACCTGGAG CTAGAGTGGT CGGTGGGGCC GATACCTGTG 
GGCGACACCT GGGGGAAGGA GGTCATCAGC CGTTTTGACA CACCGCTGGA GACAAAGGGA 
CGCTTCTACA CAGACAGCAA TGGCCGGGAG ATCCTGGAGA GGAGGCGGGA TTATCGACCC 
ACCTGGAAAC TGAACCAGAC GGAGCCCGTG GCAGGAAACT ACTATCCAGT CAACACCCGG 
ATTTACATCA CGGATGGAAA CATGCAGCTG ACTGTGCTGA CTGACCGCTC CCAGGGGGGC 
AGCAGCCTGA GAGATGGCTC GCTGGAGCTC ATGGTGCACC GAAGGCTGCT GAAGGACGAT 
GGACGCGGAG TATCGGAGCC ACTAATGGAG AACGGGTCGG GGGCGTGGGT GCGAGGGCGC 
CACCTGGTGC TGCTGGACAC AGCCCAGGCT GCAGCCGCCG GACACCGGCT CCTGGCGGAG 
CAGGAGGTCC TGGCCCCTCA GGTGGTGCTG GCCCCGGGTG GCGGCGCCGC CTACAATCTC 
GGGGCTCCTC CGCGCACGCA GTTCTCAGGG CTGCGCAGGG ACCTGCCGCC CTCGGTGCAC 
CTGCTCACGC TGGCCAGCTG GGGCCCCGAA ATGGTGCTGC TGCGCTTGGA GCACCAGTTT 
GCCGTAGGAG AGGATTCCGG ACGTAACCTG AGCGCCCCCG TTACCTTGAA CTTGAGGGAC 
CTGTTCTCCA CCTTCACCAT CACCCGCCTG CAGGAGACCA CGCTGGTGGC CAACCAGCTC 
CGCGAGGCAG CCTCCAGGCT CAAGTGGACA ACAAACACAG GCCCCACACC CCACCAAACT 
CCGTACCAGC TGGACCCGGC CAACATCACG CTGGAACCCA TGGAAATCCG CACTTTCCTG 
GCCTCAGTTC AATGGAAGGA GGTGGATGGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B1 ( XM_005259913.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259970.1 Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.

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CloneID	OHu51603
Clone ID Related Accession (Same CDS sequence)	XM_005259913.2 , XM_005259913.1
Accession Version	XM_005259913.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	lysosomal alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005259913.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGC AGGCAAAAGG AAGCAGTGTC CATGTTCTCT ACTCCACCCC CGCTTGTTAC 
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCAGTGA AACATGACGA CTTCTTCCCT 
TACGCGGATG GCCCCCACCA GTTCTGGACC GGTTACTTTT CCAGTCGGCC GGCCCTCAAA 
CGCTACGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGTG 
GGCCTGGCGG CCAACGTGGG ACCCTATGGC TCCGGAGACA GTGCACCCCT CAATGAGGCG 
ATGGCTGTGC TCCAGCATCA CGACGCCGTC AGCGGCACCT CCCGCCAGCA CGTGGCCAAC 
GACTACGCGC GCCAGCTTGC GGCAGGCTGG GGGCCTTGCG AGGTTCTTCT GAGCAACGCG 
CTGGCGCGGC TCAGAGGCTT CAAAGATCAC TTCACCTTTT GCCAACAGCT AAACATCAGC 
ATCTGCCCGC TCAGCCAGAC GGCGGCGCGC TTCCAGGTCA TCGTTTATAA TCCCCTGGGG 
CGGAAGGTGA ATTGGATGGT ACGGCTGCCG GTCAGCGAAG GCGTTTTCGT TGTGAAGGAC 
CCCAATGGCA GGACAGTGCC CAGCGATGTG GTAATATTTC CCAGCTCAGA CAGCCAGGCG 
CACCCTCCGG AGCTGCTGTT CTCAGCCTCA CTGCCCGCCC TGGGCTTCAG CACCTATTCA 
GTAGCCCAGG TGCCTCGCTG GAAGCCCCAG GCCCGCGCAC CACAGCCCAT CCCCAGAAGA 
TCCTGGTCCC CTGCTTTAAC CATCGAAAAT GAGCACATCC GGGCAACGTT TGATCCTGAC 
ACAGGGCTGT TGATGGAGAT TATGAACATG AATCAGCAAC TCCTGCTGCC TGTTCGCCAG 
ACCTTCTTCT GGTACAACGC CAGTATAGGT GACAACGAAA GTGACCAGGC CTCAGGTGCC 
TACATCTTCA GACCCAACCA ACAGAAACCG CTGCCTGTGA GCCGCTGGGC TCAGATCCAC 
CTGGTGAAGA CACCCTTGGT GCAGGAGGTG CACCAGAACT TCTCAGCTTG GTGTTCCCAG 
GTGGTTCGCC TGTACCCAGG ACAGCGGCAC CTGGAGCTAG AGTGGTCGGT GGGGCCGATA 
CCTGTGGGCG ACACCTGGGG GAAGGAGGTC ATCAGCCGTT TTGACACACC GCTGGAGACA 
AAGGGACGCT TCTACACAGA CAGCAATGGC CGGGAGATCC TGGAGAGGAG GCGGGATTAT 
CGACCCACCT GGAAACTGAA CCAGACGGAG CCCGTGGCAG GAAACTACTA TCCAGTCAAC 
ACCCGGATTT ACATCACGGA TGGAAACATG CAGCTGACTG TGCTGACTGA CCGCTCCCAG 
GGGGGCAGCA GCCTGAGAGA TGGCTCGCTG GAGCTCATGG TGCACCGAAG GCTGCTGAAG 
GACGATGGAC GCGGAGTATC GGAGCCACTA ATGGAGAACG GGTCGGGGGC GTGGGTGCGA 
GGGCGCCACC TGGTGCTGCT GGACACAGCC CAGGCTGCAG CCGCCGGACA CCGGCTCCTG 
GCGGAGCAGG AGGTCCTGGC CCCTCAGGTG GTGCTGGCCC CGGGTGGCGG CGCCGCCTAC 
AATCTCGGGG CTCCTCCGCG CACGCAGTTC TCAGGGCTGC GCAGGGACCT GCCGCCCTCG 
GTGCACCTGC TCACGCTGGC CAGCTGGGGC CCCGAAATGG TGCTGCTGCG CTTGGAGCAC 
CAGTTTGCCG TAGGAGAGGA TTCCGGACGT AACCTGAGCG CCCCCGTTAC CTTGAACTTG 
AGGGACCTGT TCTCCACCTT CACCATCACC CGCCTGCAGG AGACCACGCT GGTGGCCAAC 
CAGCTCCGCG AGGCAGCCTC CAGGCTCAAG TGGACAACAA ACACAGGCCC CACACCCCAC 
CAAACTCCGT ACCAGCTGGA CCCGGCCAAC ATCACGCTGG AACCCATGGA AATCCGCACT 
TTCCTGGCCT CAGTTCAATG GAAGGAGGTG GATGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259970.1
CDS	42..3080
Translation	MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Target ORF information:

RefSeq Version	XM_005259913.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259913.2

ORF Insert Sequence:

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGC AGGCAAAAGG AAGCAGTGTC CATGTTCTCT ACTCCACCCC CGCTTGTTAC 
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCAGTGA AACATGACGA CTTCTTCCCT 
TACGCGGATG GCCCCCACCA GTTCTGGACC GGTTACTTTT CCAGTCGGCC GGCCCTCAAA 
CGCTACGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGTG 
GGCCTGGCGG CCAACGTGGG ACCCTATGGC TCCGGAGACA GTGCACCCCT CAATGAGGCG 
ATGGCTGTGC TCCAGCATCA CGACGCCGTC AGCGGCACCT CCCGCCAGCA CGTGGCCAAC 
GACTACGCGC GCCAGCTTGC GGCAGGCTGG GGGCCTTGCG AGGTTCTTCT GAGCAACGCG 
CTGGCGCGGC TCAGAGGCTT CAAAGATCAC TTCACCTTTT GCCAACAGCT AAACATCAGC 
ATCTGCCCGC TCAGCCAGAC GGCGGCGCGC TTCCAGGTCA TCGTTTATAA TCCCCTGGGG 
CGGAAGGTGA ATTGGATGGT ACGGCTGCCG GTCAGCGAAG GCGTTTTCGT TGTGAAGGAC 
CCCAATGGCA GGACAGTGCC CAGCGATGTG GTAATATTTC CCAGCTCAGA CAGCCAGGCG 
CACCCTCCGG AGCTGCTGTT CTCAGCCTCA CTGCCCGCCC TGGGCTTCAG CACCTATTCA 
GTAGCCCAGG TGCCTCGCTG GAAGCCCCAG GCCCGCGCAC CACAGCCCAT CCCCAGAAGA 
TCCTGGTCCC CTGCTTTAAC CATCGAAAAT GAGCACATCC GGGCAACGTT TGATCCTGAC 
ACAGGGCTGT TGATGGAGAT TATGAACATG AATCAGCAAC TCCTGCTGCC TGTTCGCCAG 
ACCTTCTTCT GGTACAACGC CAGTATAGGT GACAACGAAA GTGACCAGGC CTCAGGTGCC 
TACATCTTCA GACCCAACCA ACAGAAACCG CTGCCTGTGA GCCGCTGGGC TCAGATCCAC 
CTGGTGAAGA CACCCTTGGT GCAGGAGGTG CACCAGAACT TCTCAGCTTG GTGTTCCCAG 
GTGGTTCGCC TGTACCCAGG ACAGCGGCAC CTGGAGCTAG AGTGGTCGGT GGGGCCGATA 
CCTGTGGGCG ACACCTGGGG GAAGGAGGTC ATCAGCCGTT TTGACACACC GCTGGAGACA 
AAGGGACGCT TCTACACAGA CAGCAATGGC CGGGAGATCC TGGAGAGGAG GCGGGATTAT 
CGACCCACCT GGAAACTGAA CCAGACGGAG CCCGTGGCAG GAAACTACTA TCCAGTCAAC 
ACCCGGATTT ACATCACGGA TGGAAACATG CAGCTGACTG TGCTGACTGA CCGCTCCCAG 
GGGGGCAGCA GCCTGAGAGA TGGCTCGCTG GAGCTCATGG TGCACCGAAG GCTGCTGAAG 
GACGATGGAC GCGGAGTATC GGAGCCACTA ATGGAGAACG GGTCGGGGGC GTGGGTGCGA 
GGGCGCCACC TGGTGCTGCT GGACACAGCC CAGGCTGCAG CCGCCGGACA CCGGCTCCTG 
GCGGAGCAGG AGGTCCTGGC CCCTCAGGTG GTGCTGGCCC CGGGTGGCGG CGCCGCCTAC 
AATCTCGGGG CTCCTCCGCG CACGCAGTTC TCAGGGCTGC GCAGGGACCT GCCGCCCTCG 
GTGCACCTGC TCACGCTGGC CAGCTGGGGC CCCGAAATGG TGCTGCTGCG CTTGGAGCAC 
CAGTTTGCCG TAGGAGAGGA TTCCGGACGT AACCTGAGCG CCCCCGTTAC CTTGAACTTG 
AGGGACCTGT TCTCCACCTT CACCATCACC CGCCTGCAGG AGACCACGCT GGTGGCCAAC 
CAGCTCCGCG AGGCAGCCTC CAGGCTCAAG TGGACAACAA ACACAGGCCC CACACCCCAC 
CAAACTCCGT ACCAGCTGGA CCCGGCCAAC ATCACGCTGG AACCCATGGA AATCCGCACT 
TTCCTGGCCT CAGTTCAATG GAAGGAGGTG GATGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAN2B1 ( XM_005259913.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_005259970.1 Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.

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CloneID	OHu51603
Clone ID Related Accession (Same CDS sequence)	XM_005259913.2 , XM_005259913.1
Accession Version	XM_005259913.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3039bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	lysosomal alpha-mannosidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGC AGGCAAAAGG AAGCAGTGTC CATGTTCTCT ACTCCACCCC CGCTTGTTAC 
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCAGTGA AACATGACGA CTTCTTCCCT 
TACGCGGATG GCCCCCACCA GTTCTGGACC GGTTACTTTT CCAGTCGGCC GGCCCTCAAA 
CGCTACGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGTG 
GGCCTGGCGG CCAACGTGGG ACCCTATGGC TCCGGAGACA GTGCACCCCT CAATGAGGCG 
ATGGCTGTGC TCCAGCATCA CGACGCCGTC AGCGGCACCT CCCGCCAGCA CGTGGCCAAC 
GACTACGCGC GCCAGCTTGC GGCAGGCTGG GGGCCTTGCG AGGTTCTTCT GAGCAACGCG 
CTGGCGCGGC TCAGAGGCTT CAAAGATCAC TTCACCTTTT GCCAACAGCT AAACATCAGC 
ATCTGCCCGC TCAGCCAGAC GGCGGCGCGC TTCCAGGTCA TCGTTTATAA TCCCCTGGGG 
CGGAAGGTGA ATTGGATGGT ACGGCTGCCG GTCAGCGAAG GCGTTTTCGT TGTGAAGGAC 
CCCAATGGCA GGACAGTGCC CAGCGATGTG GTAATATTTC CCAGCTCAGA CAGCCAGGCG 
CACCCTCCGG AGCTGCTGTT CTCAGCCTCA CTGCCCGCCC TGGGCTTCAG CACCTATTCA 
GTAGCCCAGG TGCCTCGCTG GAAGCCCCAG GCCCGCGCAC CACAGCCCAT CCCCAGAAGA 
TCCTGGTCCC CTGCTTTAAC CATCGAAAAT GAGCACATCC GGGCAACGTT TGATCCTGAC 
ACAGGGCTGT TGATGGAGAT TATGAACATG AATCAGCAAC TCCTGCTGCC TGTTCGCCAG 
ACCTTCTTCT GGTACAACGC CAGTATAGGT GACAACGAAA GTGACCAGGC CTCAGGTGCC 
TACATCTTCA GACCCAACCA ACAGAAACCG CTGCCTGTGA GCCGCTGGGC TCAGATCCAC 
CTGGTGAAGA CACCCTTGGT GCAGGAGGTG CACCAGAACT TCTCAGCTTG GTGTTCCCAG 
GTGGTTCGCC TGTACCCAGG ACAGCGGCAC CTGGAGCTAG AGTGGTCGGT GGGGCCGATA 
CCTGTGGGCG ACACCTGGGG GAAGGAGGTC ATCAGCCGTT TTGACACACC GCTGGAGACA 
AAGGGACGCT TCTACACAGA CAGCAATGGC CGGGAGATCC TGGAGAGGAG GCGGGATTAT 
CGACCCACCT GGAAACTGAA CCAGACGGAG CCCGTGGCAG GAAACTACTA TCCAGTCAAC 
ACCCGGATTT ACATCACGGA TGGAAACATG CAGCTGACTG TGCTGACTGA CCGCTCCCAG 
GGGGGCAGCA GCCTGAGAGA TGGCTCGCTG GAGCTCATGG TGCACCGAAG GCTGCTGAAG 
GACGATGGAC GCGGAGTATC GGAGCCACTA ATGGAGAACG GGTCGGGGGC GTGGGTGCGA 
GGGCGCCACC TGGTGCTGCT GGACACAGCC CAGGCTGCAG CCGCCGGACA CCGGCTCCTG 
GCGGAGCAGG AGGTCCTGGC CCCTCAGGTG GTGCTGGCCC CGGGTGGCGG CGCCGCCTAC 
AATCTCGGGG CTCCTCCGCG CACGCAGTTC TCAGGGCTGC GCAGGGACCT GCCGCCCTCG 
GTGCACCTGC TCACGCTGGC CAGCTGGGGC CCCGAAATGG TGCTGCTGCG CTTGGAGCAC 
CAGTTTGCCG TAGGAGAGGA TTCCGGACGT AACCTGAGCG CCCCCGTTAC CTTGAACTTG 
AGGGACCTGT TCTCCACCTT CACCATCACC CGCCTGCAGG AGACCACGCT GGTGGCCAAC 
CAGCTCCGCG AGGCAGCCTC CAGGCTCAAG TGGACAACAA ACACAGGCCC CACACCCCAC 
CAAACTCCGT ACCAGCTGGA CCCGGCCAAC ATCACGCTGG AACCCATGGA AATCCGCACT 
TTCCTGGCCT CAGTTCAATG GAAGGAGGTG GATGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005259970.1
CDS	55..3093
Translation	MGAYARASGVCARGCLDSAGPWTMSRALRPPLPPLCFFLLLLAAAGARAGGYETCPTVQPNMLNVHLLPHTHDDVGWLKTVDQYFYGIKNDIQHAGVQYILDSVISALLADPTRRFIYVEIAFFSRWWHQQTNATQEVVRDLVRQGRLEFANGGWVMNDEAATHYGAIVDQMTLGLRFLEDTFGNDGRPRVAWHIDPFGHSREQASLFAQMGFDGFFFGRLDYQDKWVRMQKLEMEQVWRASTSLKPPTADLFTGVLPNGYNPPRNLCWDVLCVDQPLVEDPRSPEYNAKELVDYFLNVATAQGRYYRTNHTVMTMGSDFQYENANMWFKNLDKLIRLVNAQQQAKGSSVHVLYSTPACYLWELNKANLTWSVKHDDFFPYADGPHQFWTGYFSSRPALKRYERLSYNFLQVCNQLEALVGLAANVGPYGSGDSAPLNEAMAVLQHHDAVSGTSRQHVANDYARQLAAGWGPCEVLLSNALARLRGFKDHFTFCQQLNISICPLSQTAARFQVIVYNPLGRKVNWMVRLPVSEGVFVVKDPNGRTVPSDVVIFPSSDSQAHPPELLFSASLPALGFSTYSVAQVPRWKPQARAPQPIPRRSWSPALTIENEHIRATFDPDTGLLMEIMNMNQQLLLPVRQTFFWYNASIGDNESDQASGAYIFRPNQQKPLPVSRWAQIHLVKTPLVQEVHQNFSAWCSQVVRLYPGQRHLELEWSVGPIPVGDTWGKEVISRFDTPLETKGRFYTDSNGREILERRRDYRPTWKLNQTEPVAGNYYPVNTRIYITDGNMQLTVLTDRSQGGSSLRDGSLELMVHRRLLKDDGRGVSEPLMENGSGAWVRGRHLVLLDTAQAAAAGHRLLAEQEVLAPQVVLAPGGGAAYNLGAPPRTQFSGLRRDLPPSVHLLTLASWGPEMVLLRLEHQFAVGEDSGRNLSAPVTLNLRDLFSTFTITRLQETTLVANQLREAASRLKWTTNTGPTPHQTPYQLDPANITLEPMEIRTFLASVQWKEVDG

Target ORF information:

RefSeq Version	XM_005259913.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mannosidase alpha class 2B member 1 (MAN2B1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005259913.1

ORF Insert Sequence:

ATGGGCGCCT ACGCGCGGGC TTCGGGGGTC TGCGCTCGCG GCTGCCTGGA CTCAGCAGGC 
CCCTGGACCA TGTCCCGCGC CCTGCGGCCA CCGCTCCCGC CTCTCTGCTT TTTCCTTTTG 
TTGCTGGCGG CTGCCGGTGC TCGGGCCGGG GGATACGAGA CATGCCCCAC AGTGCAGCCG 
AACATGCTGA ACGTGCACCT GCTGCCTCAC ACACATGATG ACGTGGGCTG GCTCAAAACC 
GTGGACCAGT ACTTTTATGG AATCAAGAAT GACATCCAGC ACGCCGGTGT GCAGTACATC 
CTGGACTCGG TCATCTCTGC CTTGCTGGCA GATCCCACCC GTCGCTTCAT TTACGTGGAG 
ATTGCCTTCT TCTCCCGTTG GTGGCACCAG CAGACAAATG CCACACAGGA AGTCGTGCGA 
GACCTTGTGC GCCAGGGGCG CCTGGAGTTC GCCAATGGTG GCTGGGTGAT GAACGATGAG 
GCAGCCACCC ACTACGGTGC CATCGTGGAC CAGATGACAC TTGGGCTGCG CTTTCTGGAG 
GACACATTTG GCAATGATGG GCGACCCCGT GTGGCCTGGC ACATTGACCC CTTCGGCCAC 
TCTCGGGAGC AGGCCTCGCT GTTTGCGCAG ATGGGCTTCG ACGGCTTCTT CTTTGGGCGC 
CTTGATTATC AAGATAAGTG GGTACGGATG CAGAAGCTGG AGATGGAGCA GGTGTGGCGG 
GCCAGCACCA GCCTGAAGCC CCCGACCGCG GACCTCTTCA CTGGTGTGCT TCCCAATGGT 
TACAACCCGC CAAGGAATCT GTGCTGGGAT GTGCTGTGTG TCGATCAGCC GCTGGTGGAG 
GACCCTCGCA GCCCCGAGTA CAACGCCAAG GAGCTGGTCG ATTACTTCCT AAATGTGGCC 
ACTGCCCAGG GCCGGTATTA CCGCACCAAC CACACTGTGA TGACCATGGG CTCGGACTTC 
CAATATGAGA ATGCCAACAT GTGGTTCAAG AACCTTGACA AGCTCATCCG GCTGGTAAAT 
GCGCAGCAGC AGGCAAAAGG AAGCAGTGTC CATGTTCTCT ACTCCACCCC CGCTTGTTAC 
CTCTGGGAGC TGAACAAGGC CAACCTCACC TGGTCAGTGA AACATGACGA CTTCTTCCCT 
TACGCGGATG GCCCCCACCA GTTCTGGACC GGTTACTTTT CCAGTCGGCC GGCCCTCAAA 
CGCTACGAGC GCCTCAGCTA CAACTTCCTG CAGGTGTGCA ACCAGCTGGA GGCGCTGGTG 
GGCCTGGCGG CCAACGTGGG ACCCTATGGC TCCGGAGACA GTGCACCCCT CAATGAGGCG 
ATGGCTGTGC TCCAGCATCA CGACGCCGTC AGCGGCACCT CCCGCCAGCA CGTGGCCAAC 
GACTACGCGC GCCAGCTTGC GGCAGGCTGG GGGCCTTGCG AGGTTCTTCT GAGCAACGCG 
CTGGCGCGGC TCAGAGGCTT CAAAGATCAC TTCACCTTTT GCCAACAGCT AAACATCAGC 
ATCTGCCCGC TCAGCCAGAC GGCGGCGCGC TTCCAGGTCA TCGTTTATAA TCCCCTGGGG 
CGGAAGGTGA ATTGGATGGT ACGGCTGCCG GTCAGCGAAG GCGTTTTCGT TGTGAAGGAC 
CCCAATGGCA GGACAGTGCC CAGCGATGTG GTAATATTTC CCAGCTCAGA CAGCCAGGCG 
CACCCTCCGG AGCTGCTGTT CTCAGCCTCA CTGCCCGCCC TGGGCTTCAG CACCTATTCA 
GTAGCCCAGG TGCCTCGCTG GAAGCCCCAG GCCCGCGCAC CACAGCCCAT CCCCAGAAGA 
TCCTGGTCCC CTGCTTTAAC CATCGAAAAT GAGCACATCC GGGCAACGTT TGATCCTGAC 
ACAGGGCTGT TGATGGAGAT TATGAACATG AATCAGCAAC TCCTGCTGCC TGTTCGCCAG 
ACCTTCTTCT GGTACAACGC CAGTATAGGT GACAACGAAA GTGACCAGGC CTCAGGTGCC 
TACATCTTCA GACCCAACCA ACAGAAACCG CTGCCTGTGA GCCGCTGGGC TCAGATCCAC 
CTGGTGAAGA CACCCTTGGT GCAGGAGGTG CACCAGAACT TCTCAGCTTG GTGTTCCCAG 
GTGGTTCGCC TGTACCCAGG ACAGCGGCAC CTGGAGCTAG AGTGGTCGGT GGGGCCGATA 
CCTGTGGGCG ACACCTGGGG GAAGGAGGTC ATCAGCCGTT TTGACACACC GCTGGAGACA 
AAGGGACGCT TCTACACAGA CAGCAATGGC CGGGAGATCC TGGAGAGGAG GCGGGATTAT 
CGACCCACCT GGAAACTGAA CCAGACGGAG CCCGTGGCAG GAAACTACTA TCCAGTCAAC 
ACCCGGATTT ACATCACGGA TGGAAACATG CAGCTGACTG TGCTGACTGA CCGCTCCCAG 
GGGGGCAGCA GCCTGAGAGA TGGCTCGCTG GAGCTCATGG TGCACCGAAG GCTGCTGAAG 
GACGATGGAC GCGGAGTATC GGAGCCACTA ATGGAGAACG GGTCGGGGGC GTGGGTGCGA 
GGGCGCCACC TGGTGCTGCT GGACACAGCC CAGGCTGCAG CCGCCGGACA CCGGCTCCTG 
GCGGAGCAGG AGGTCCTGGC CCCTCAGGTG GTGCTGGCCC CGGGTGGCGG CGCCGCCTAC 
AATCTCGGGG CTCCTCCGCG CACGCAGTTC TCAGGGCTGC GCAGGGACCT GCCGCCCTCG 
GTGCACCTGC TCACGCTGGC CAGCTGGGGC CCCGAAATGG TGCTGCTGCG CTTGGAGCAC 
CAGTTTGCCG TAGGAGAGGA TTCCGGACGT AACCTGAGCG CCCCCGTTAC CTTGAACTTG 
AGGGACCTGT TCTCCACCTT CACCATCACC CGCCTGCAGG AGACCACGCT GGTGGCCAAC 
CAGCTCCGCG AGGCAGCCTC CAGGCTCAAG TGGACAACAA ACACAGGCCC CACACCCCAC 
CAAACTCCGT ACCAGCTGGA CCCGGCCAAC ATCACGCTGG AACCCATGGA AATCCGCACT 
TTCCTGGCCT CAGTTCAATG GAAGGAGGTG GATGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Lysosomal storage disease in the brain: mutations of the ?-mannosidase gene identified in autosomal dominant nystagmus.
Genetics in medicine : official journal of the American College of Medical Genetics17(12)971-9(2015 Dec)
Yu P,Cui Y,Cai W,Wu H,Xiao X,Shao Q,Ma L,Guo S,Wu N,Jin ZB,Wang Y,Cai T,Sun ZS,Qu J

Alpha-mannosidosis: correlation between phenotype, genotype and mutant MAN2B1 subcellular localisation.
Orphanet journal of rare diseases1070(2015 Jun)
Borgwardt L,Stensland HM,Olsen KJ,Wibrand F,Klenow HB,Beck M,Amraoui Y,Arash L,Fogh J,Nilssen ?,Dali CI,Lund AM

Human neutrophils secrete bioactive paucimannosidic proteins from azurophilic granules into pathogen-infected sputum.
The Journal of biological chemistry290(14)8789-802(2015 Apr)
Thaysen-Andersen M,Venkatakrishnan V,Loke I,Laurini C,Diestel S,Parker BL,Packer NH

Molecular diagnosis of a Chinese pedigree with ?-mannosidosis and identification of a novel missense mutation.
Journal of pediatric endocrinology & metabolism : JPEM27(5-6)491-5(2014 May)
Wu X,Pan J,Guo Y,Guo C,Jiang W,Li R,Tang J,Ai Y

Genome-wide study of percent emphysema on computed tomography in the general population. The Multi-Ethnic Study of Atherosclerosis Lung/SNP Health Association Resource Study.
American journal of respiratory and critical care medicine189(4)408-18(2014 Feb)
Manichaikul A,Hoffman EA,Smolonska J,Gao W,Cho MH,Baumhauer H,Budoff M,Austin JH,Washko GR,Carr JJ,Kaufman JD,Pottinger T,Powell CA,Wijmenga C,Zanen P,Groen HJ,Postma DS,Wanner A,Rouhani FN,Brantly ML,Powell R,Smith BM,Rabinowitz D,Raffel LJ,Hinckley Stukovsky KD,Crapo JD,Beaty TH,Hokanson JE,Silverman EK,Dupuis J,O'Connor GT,Boezen HM,Rich SS,Barr RG

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MAN2B1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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