MAGEA12 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAGEA12
Entrez Gene ID	4111
Full Name	MAGE family member A12
Synonyms	CT1.12,MAGE12
General protein information	Preferred Names MAGE family member A12 Names melanoma-associated antigen 12 MAGE12F antigen cancer/testis antigen 1.12 cancer/testis antigen family 1, member 12 melanoma antigen family A, 12 melanoma antigen family A12
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq28
Summary	This gene is closely related to several other genes clustered on chromosome X. These genes may be overexpressed in tumors. Multiple alternatively spliced variants encoding the same protein have been identified. [provided by RefSeq, Jun 2014].
Disorder MIM:	300177

mRNA and Protein(s)

mRNA	Protein	Name
NM_005367.6	NP_005358.2	melanoma-associated antigen 12
NM_005367.7	NP_005358.2	melanoma-associated antigen 12
NM_001166387.4	NP_001159859.1	melanoma-associated antigen 12
NM_001166387.3	NP_001159859.1	melanoma-associated antigen 12
NM_001166386.3	NP_001159858.1	melanoma-associated antigen 12

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MAGEA12	XP_521314.2
Homo sapiens (human)	MAGEA12	NP_001159858.1

Gene Ontology
Bibliography

Related articles in PubMed

Identification of MAGEA12 as a prognostic outlier gene in gastric cancers.
Wu J, Wang J, Shen W
Neoplasma64(2)238-243(2017)

An inter-species protein-protein interaction network across vast evolutionary distance.
Zhong Q, Pevzner SJ, Hao T, Wang Y, Mosca R, Menche J, Taipale M, Ta?an M, Fan C, Yang X, Haley P, Murray RR, Mer F, Gebreab F, Tam S, MacWilliams A, Dricot A, Reichert P, Santhanam B, Ghamsari L, Calderwood MA, Rolland T, Charloteaux B, Lindquist S, Barab?si AL, Hill DE, Aloy P, Cusick ME, Xia Y, Roth FP, Vidal M
Molecular systems biology12(4)865(2016 Apr)

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Discovery and validation of DNA hypomethylation biomarkers for liver cancer using HRM-specific probes.
Stefanska B, Bouzelmat A, Huang J, Suderman M, Hallett M, Han ZG, Al-Mahtab M, Akbar SM, Khan WA, Raqib R, Szyf M
PloS one8(8)e68439(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data show the Differences in high resolution melting analysis in promoters of tumor markers neuronal membrane glycoprotein M6-B, melanoma antigen family A12 and immunoglobulin superfamily Fc receptor indicated invasiveness of hepatocellular carcinoma.
Title: Discovery and validation of DNA hypomethylation biomarkers for liver cancer using HRM-specific probes.

To identify novel CTL epitopes from MAGE-A6 and MAGE-A12 antigens.
Title: Identification of novel MAGE-A6- and MAGE-A12-derived HLA-A24-restricted cytotoxic T lymphocyte epitopes using an in silico peptide-docking assay.

Multiple simultaneous detection of MAGE-A [subtypes] more specific and sensitive than detection of single MAGE-A antigen for the diagnostic and prognostic evaluation of oral squamous cell carcinoma
Title: Expression of melanoma-associated antigens in oral squamous cell carcinoma.

results of this study may indicate Melanoma associated-A12 antigen(MAGE-A12)as a diagnostic marker for early detection of oral squamous cell carcinoma
Title: Expression of MAGE-A12 in oral squamous cell carcinoma.

These data show, for the first time, the involvement of methyl-CpG binding domain proteins in the regulation of the MAGE-A genes.
Title: Methyl-CpG binding domain proteins and their involvement in the regulation of the MAGE-A1, MAGE-A2, MAGE-A3, and MAGE-A12 gene promoters.

The following MAGEA12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAGEA12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu27949	NM_005367.7 Latest version!	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu27949	NM_001166387.4 Latest version!	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu27949	NM_001166386.3 Latest version!	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu27949	NM_005367.6	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu27949	NM_001166387.3	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAGEA12 ( NM_005367.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_005358.2 Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27949
Clone ID Related Accession (Same CDS sequence)	NM_005367.6 , NM_005367.7 , NM_001166387.4 , NM_001166387.3 , NM_001166386.3
Accession Version	NM_005367.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-15
Organism	Homo sapiens(human)
Product	melanoma-associated antigen 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. This sequence is a reference standard in the RefSeqGene project. On Apr 5, 2014 this sequence version replaced NM_005367.5. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003408.2, BX341218.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005358.2
CDS	157..1101
Translation	MPLEQRSQHCKPEEGLEAQGEALGLVGAQAPATEEQETASSSSTLVEVTLREVPAAESPSPPHSPQGASTLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFLLLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVEVVRIGHLYILVTCLGLSYDGLLGDNQIVPKTGLLIIVLAIIAKEGDCAPEEKIWEELSVLEASDGREDSVFAHPRKLLTQDLVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHLLKISGGPHISYPPLHEWAFREGEE

Target ORF information:

RefSeq Version	NM_005367.6
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005367.6

ORF Insert Sequence:

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGEA12 ( NM_005367.7 ) cDNA ORF clone, Homo sapiens(human) -> NP_005358.2 Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu27949
Clone ID Related Accession (Same CDS sequence)	NM_005367.6 , NM_005367.7 , NM_001166387.4 , NM_001166387.3 , NM_001166386.3
Accession Version	NM_005367.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	melanoma-associated antigen 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_005367.6. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC003408.2, SRR5189661.54901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005358.2
CDS	179..1123
Translation	MPLEQRSQHCKPEEGLEAQGEALGLVGAQAPATEEQETASSSSTLVEVTLREVPAAESPSPPHSPQGASTLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFLLLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVEVVRIGHLYILVTCLGLSYDGLLGDNQIVPKTGLLIIVLAIIAKEGDCAPEEKIWEELSVLEASDGREDSVFAHPRKLLTQDLVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHLLKISGGPHISYPPLHEWAFREGEE

Target ORF information:

RefSeq Version	NM_005367.7
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005367.7

ORF Insert Sequence:

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGEA12 ( NM_001166387.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159859.1 Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27949
Clone ID Related Accession (Same CDS sequence)	NM_005367.6 , NM_005367.7 , NM_001166387.4 , NM_001166387.3 , NM_001166386.3
Accession Version	NM_001166387.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	melanoma-associated antigen 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. On Nov 22, 2018 this sequence version replaced NM_001166387.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.54033.1, SRR5189661.114417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000393869.8/ ENSP00000377447.3 RefSeq Select criteria :: based on expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159859.1
CDS	285..1229
Translation	MPLEQRSQHCKPEEGLEAQGEALGLVGAQAPATEEQETASSSSTLVEVTLREVPAAESPSPPHSPQGASTLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFLLLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVEVVRIGHLYILVTCLGLSYDGLLGDNQIVPKTGLLIIVLAIIAKEGDCAPEEKIWEELSVLEASDGREDSVFAHPRKLLTQDLVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHLLKISGGPHISYPPLHEWAFREGEE

Target ORF information:

RefSeq Version	NM_001166387.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166387.4

ORF Insert Sequence:

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGEA12 ( NM_001166387.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159859.1 Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu27949
Clone ID Related Accession (Same CDS sequence)	NM_005367.6 , NM_005367.7 , NM_001166387.4 , NM_001166387.3 , NM_001166386.3
Accession Version	NM_001166387.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	melanoma-associated antigen 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. On Apr 6, 2014 this sequence version replaced NM_001166387.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC003408.2, SRR1660807.288829.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159859.1
CDS	263..1207
Translation	MPLEQRSQHCKPEEGLEAQGEALGLVGAQAPATEEQETASSSSTLVEVTLREVPAAESPSPPHSPQGASTLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFLLLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVEVVRIGHLYILVTCLGLSYDGLLGDNQIVPKTGLLIIVLAIIAKEGDCAPEEKIWEELSVLEASDGREDSVFAHPRKLLTQDLVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHLLKISGGPHISYPPLHEWAFREGEE

Target ORF information:

RefSeq Version	NM_001166387.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166387.3

ORF Insert Sequence:

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAGEA12 ( NM_001166386.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001159858.1 Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu27949
Clone ID Related Accession (Same CDS sequence)	NM_005367.6 , NM_005367.7 , NM_001166387.4 , NM_001166387.3 , NM_001166386.3
Accession Version	NM_001166386.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 945bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	melanoma-associated antigen 12
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC244102.3. On Apr 5, 2014 this sequence version replaced NM_001166386.2. Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2 and 3 all encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314168.1, BI861455.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001159858.1
CDS	355..1299
Translation	MPLEQRSQHCKPEEGLEAQGEALGLVGAQAPATEEQETASSSSTLVEVTLREVPAAESPSPPHSPQGASTLPTTINYTLWSQSDEGSSNEEQEGPSTFPDLETSFQVALSRKMAELVHFLLLKYRAREPFTKAEMLGSVIRNFQDFFPVIFSKASEYLQLVFGIEVVEVVRIGHLYILVTCLGLSYDGLLGDNQIVPKTGLLIIVLAIIAKEGDCAPEEKIWEELSVLEASDGREDSVFAHPRKLLTQDLVQENYLEYRQVPGSDPACYEFLWGPRALVETSYVKVLHHLLKISGGPHISYPPLHEWAFREGEE

Target ORF information:

RefSeq Version	NM_001166386.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAGE family member A12 (MAGEA12), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166386.3

ORF Insert Sequence:

ATGCCACTTG AGCAGAGGAG TCAGCACTGC AAGCCTGAGG AAGGCCTTGA GGCCCAAGGA 
GAGGCCCTGG GCTTGGTGGG TGCGCAGGCT CCTGCTACTG AGGAGCAGGA GACTGCCTCC 
TCCTCCTCTA CTCTAGTGGA AGTCACCCTG CGGGAGGTGC CTGCTGCCGA GTCACCAAGT 
CCTCCCCACA GTCCTCAGGG AGCCTCCACC CTCCCCACTA CCATCAACTA TACTCTCTGG 
AGTCAATCCG ATGAGGGCTC CAGCAACGAA GAACAGGAAG GGCCAAGCAC CTTTCCTGAC 
CTGGAGACGA GCTTCCAAGT AGCACTCAGT AGGAAGATGG CTGAGTTGGT TCATTTTCTG 
CTCCTCAAGT ATCGAGCCAG GGAGCCATTC ACAAAGGCAG AAATGCTGGG GAGTGTCATC 
AGAAATTTCC AGGACTTCTT TCCTGTGATC TTCAGCAAAG CCTCCGAGTA CTTGCAGCTG 
GTCTTTGGCA TCGAGGTGGT GGAAGTGGTC CGCATCGGCC ACTTGTACAT CCTTGTCACC 
TGCCTGGGCC TCTCCTACGA TGGCCTGCTG GGCGACAATC AGATCGTGCC CAAGACAGGC 
CTCCTGATAA TCGTCCTGGC CATAATCGCA AAAGAGGGCG ACTGTGCCCC TGAGGAGAAA 
ATCTGGGAGG AGCTGAGTGT GTTGGAGGCA TCTGATGGGA GGGAGGACAG TGTCTTTGCG 
CATCCCAGGA AGCTGCTCAC CCAAGATTTG GTGCAGGAAA ACTACCTGGA GTACCGGCAG 
GTCCCCGGCA GTGATCCTGC ATGCTACGAG TTCCTGTGGG GTCCAAGGGC CCTCGTTGAA 
ACCAGCTATG TGAAAGTCCT GCACCATTTG CTAAAGATCA GTGGAGGACC TCACATTTCC 
TACCCACCCC TGCATGAATG GGCTTTTAGA GAGGGGGAAG AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Identification of MAGEA12 as a prognostic outlier gene in gastric cancers.
Neoplasma64(2)238-243(2017)
Wu J,Wang J,Shen W

An inter-species protein-protein interaction network across vast evolutionary distance.
Molecular systems biology12(4)865(2016 Apr)
Zhong Q,Pevzner SJ,Hao T,Wang Y,Mosca R,Menche J,Taipale M,Ta?an M,Fan C,Yang X,Haley P,Murray RR,Mer F,Gebreab F,Tam S,MacWilliams A,Dricot A,Reichert P,Santhanam B,Ghamsari L,Calderwood MA,Rolland T,Charloteaux B,Lindquist S,Barab?si AL,Hill DE,Aloy P,Cusick ME,Xia Y,Roth FP,Vidal M

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Discovery and validation of DNA hypomethylation biomarkers for liver cancer using HRM-specific probes.
PloS one8(8)e68439(2013)
Stefanska B,Bouzelmat A,Huang J,Suderman M,Hallett M,Han ZG,Al-Mahtab M,Akbar SM,Khan WA,Raqib R,Szyf M

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MAGEA12 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報