BCAM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	BCAM
Entrez Gene ID	4059
Full Name	basal cell adhesion molecule (Lutheran blood group)
Synonyms	AU,CD239,LU,MSK19
General protein information	Preferred Names basal cell adhesion molecule (Lutheran blood group) Names basal cell adhesion molecule Auberger b antigen B-CAM cell surface glycoprotein B-cell adhesion molecule F8/G253 antigen Lutheran blood group variant LUGA basal cell adhesion molecule (Lu and Au blood groups)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.32
Summary	This gene encodes Lutheran blood group glycoprotein, a member of the immunoglobulin superfamily and a receptor for the extracellular matrix protein, laminin. The protein contains five extracellular immunoglobulin domains, a single transmembrane domain, and a short C-terminal cytoplasmic tail. This protein may play a role in epithelial cell cancer and in vaso-occlusion of red blood cells in sickle cell disease. Polymorphisms in this gene define some of the antigens in the Lutheran system and also the Auberger system. Inactivating variants of this gene result in the recessive Lutheran null phenotype, Lu(a-b-), of the Lutheran blood group. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012].
Disorder MIM:	612773
Disorder Html:	[Blood group, Lutheran system], 111200 (3); [Blood group, Auberger system], 111200 (3); [Blood group, Lutheran null], 247420 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013257.2	NP_001013275.1	basal cell adhesion molecule isoform 2 precursor
NM_005581.4	NP_005572.2	basal cell adhesion molecule isoform 1 precursor
NM_005581.5	NP_005572.2	basal cell adhesion molecule isoform 1 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCAM	NP_005572.2
Danio rerio (zebrafish)	bcam	NP_001070095.1
Danio rerio (zebrafish)	LOC100536698	XP_003201751.2
Pan troglodytes (chimpanzee)	BCAM	XP_001161955.3
Canis lupus familiaris (dog)	BCAM	XP_541571.4
Xenopus tropicalis (tropical clawed frog)	bcam	XP_002941084.2
Rattus norvegicus (Norway rat)	Bcam	NP_113940.1
Bos taurus (cattle)	BCAM	NP_777166.1
Mus musculus (house mouse)	Bcam	NP_065232.1
	BCAM	XP_002801340.1

Gene Ontology
Bibliography

Related articles in PubMed

Adhesive activity of Lu glycoproteins is regulated by interaction with spectrin.
An X, Gauthier E, Zhang X, Guo X, Anstee DJ, Mohandas N, Chasis JA
Blood112(13)5212-8(2008 Dec)

The Laminin 511/521-binding site on the Lutheran blood group glycoprotein is located at the flexible junction of Ig domains 2 and 3.
Mankelow TJ, Burton N, Stefansdottir FO, Spring FA, Parsons SF, Pedersen JS, Oliveira CL, Lammie D, Wess T, Mohandas N, Chasis JA, Brady RL, Anstee DJ
Blood110(9)3398-406(2007 Nov)

Characterization of the laminin binding domains of the Lutheran blood group glycoprotein.
El Nemer W, Gane P, Colin Y, D'Ambrosio AM, Callebaut I, Cartron JP, Van Kim CL
The Journal of biological chemistry276(26)23757-62(2001 Jun)

Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind alpha5 chain-containing human laminin with high affinity.
Parsons SF, Lee G, Spring FA, Willig TN, Peters LL, Gimm JA, Tanner MJ, Mohandas N, Anstee DJ, Chasis JA
Blood97(1)312-20(2001 Jan)

The Lutheran blood group glycoproteins, the erythroid receptors for laminin, are adhesion molecules.
El Nemer W, Gane P, Colin Y, Bony V, Rahuel C, Galact?ros F, Cartron JP, Le Van Kim C
The Journal of biological chemistry273(27)16686-93(1998 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The serum levels of IL-8, MIP-1 alpha, MIP-1 beta, MMP-8, Resistin, FLRG, and BCAM were significantly higher in breast cancer patients, but LAP and TSH-beta levels were lower.
Title: Serum cytokine profile in patients with breast cancer.

data show that the BCAM/LAMA5 system plays a functional role in the metastatic spreading of KRAS-mutant colorectal cancer
Title: BCAM and LAMA5 Mediate the Recognition between Tumor Cells and the Endothelium in the Metastatic Spreading of KRAS-Mutant Colorectal Cancer.

four new alleles with coding sequence variants were identified in the LU gene
Title: Identification of new KLF1 and LU alleles during the resolution of Lutheran typing discrepancies.

The variable levels of expression of mutated JAK2 and BCAM proteins determine different adhesion patterns of erythrocytes from polycythemia vera patients.
Title: Lu/BCAM-mediated cell adhesion as biological marker of JAK2V617F activity in erythrocytes of polycythemia vera patients.

Cytometry analysis evidenced a specific expression profile on reticulocytes of SCA infants, with notably an increased expression of the adhesion molecules Lu/BCAM, ICAM-4 and LFA-3, both in percentage of positive cells and in surface density.
Title: Erythroid Adhesion Molecules in Sickle Cell Anaemia Infants: Insights Into Early Pathophysiology.

The following BCAM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the BCAM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07730	NM_001013257.2 Latest version!	Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu20355	NM_005581.5 Latest version!	Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu20355	NM_005581.4	Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

BCAM ( NM_001013257.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001013275.1 Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07730
Clone ID Related Accession (Same CDS sequence)	NM_001013257.2
Accession Version	NM_001013257.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	basal cell adhesion molecule isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP352029.1, X80026.1, AC092306.2, X83425.1 and BM985314.1. This sequence is a reference standard in the RefSeqGene project. On May 6, 2012 this sequence version replaced NM_001013257.1. Transcript Variant: This variant (2) includes an additional segment in its 3' coding region, which results in an early stop codon, compared to variant 1. The encoded isoform (2) is shorter at the C-terminus, compared to isoform 1. The full-length nature of this variant is supported by data in PMIDs 8781446 and 9192786. Sequence Note: This RefSeq record represents the LU*0010101 allele. This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS ID represents the BCAM transcript variant described in PMIDs 8781446 and 9192786. It encodes the C-terminally truncated 78 kDa isoform described in PMIDs 3810828 and 7777537. The transcript is a candidate for nonsense-mediated mRNA decay (NMD), but the protein is represented because this is the only currently available transcript variant that can encode the 78 kDa isoform. This isoform was detected by immunoblotting in PMID:3810828, and the authors of PMID:7777537 indicate that this isoform was not detected when C-terminal antibodies were used, but it was when antibodies from the region in common with the 85 kDa isoform (CCDS12644.1 representation) were used, thereby suggesting that this C-terminally truncated isoform is valid. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: X80026.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: PMID: 8781446, 9192786 NMD candidate :: PMID: 7777537 ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCC CGGACGCACC GGCCCAGGCG CGCGGGGCCC CGCGGCTGCT GTTGCTCGCA 
GTCCTGCTGG CGGCGCACCC AGATGCCCAG GCGGAGGTGC GCTTGTCTGT ACCCCCGCTG 
GTGGAGGTGA TGCGAGGAAA GTCTGTCATT CTGGACTGCA CCCCTACGGG AACCCACGAC 
CATTATATGC TGGAATGGTT CCTTACCGAC CGCTCGGGAG CTCGCCCCCG CCTAGCCTCG 
GCTGAGATGC AGGGCTCTGA GCTCCAGGTC ACAATGCACG ACACCCGGGG CCGCAGTCCC 
CCATACCAGC TGGACTCCCA GGGGCGCCTG GTGCTGGCTG AGGCCCAGGT GGGCGACGAG 
CGAGACTACG TGTGCGTGGT GAGGGCAGGG GCGGCAGGCA CTGCTGAGGC CACTGCGCGG 
CTCAACGTGT TTGCAAAGCC AGAGGCCACT GAGGTCTCCC CCAACAAAGG GACACTGTCT 
GTGATGGAGG ACTCTGCCCA GGAGATCGCC ACCTGCAACA GCCGGAACGG GAACCCGGCC 
CCCAAGATCA CGTGGTATCG CAACGGGCAG CGCCTGGAGG TGCCCGTAGA GATGAACCCA 
GAGGGCTACA TGACCAGCCG CACGGTCCGG GAGGCCTCGG GCCTGCTCTC CCTCACCAGC 
ACCCTCTACC TGCGGCTCCG CAAGGATGAC CGAGACGCCA GCTTCCACTG CGCCGCCCAC 
TACAGCCTGC CCGAGGGCCG CCACGGCCGC CTGGACAGCC CCACCTTCCA CCTCACCCTG 
CACTATCCCA CGGAGCACGT GCAGTTCTGG GTGGGCAGCC CGTCCACCCC AGCAGGCTGG 
GTACGCGAGG GTGACACTGT CCAGCTGCTC TGCCGGGGGG ACGGCAGCCC CAGCCCGGAG 
TATACGCTTT TCCGCCTTCA GGATGAGCAG GAGGAAGTGC TGAATGTGAA TCTCGAGGGG 
AACTTGACCC TGGAGGGAGT GACCCGGGGC CAGAGCGGGA CCTATGGCTG CAGAGTGGAG 
GATTACGACG CGGCAGATGA CGTGCAGCTC TCCAAGACGC TGGAGCTGCG CGTGGCCTAT 
CTGGACCCCC TGGAGCTCAG CGAGGGGAAG GTGCTTTCCT TACCTCTAAA CAGCAGTGCA 
GTCGTGAACT GCTCCGTGCA CGGCCTGCCC ACCCCTGCCC TACGCTGGAC CAAGGACTCC 
ACTCCCCTGG GCGATGGCCC CATGCTGTCG CTCAGTTCTA TCACCTTCGA TTCCAATGGC 
ACCTACGTAT GTGAGGCCTC CCTGCCCACA GTCCCGGTCC TCAGCCGCAC CCAGAACTTC 
ACGCTGCTGG TCCAAGGCTC GCCAGAGCTA AAGACAGCGG AAATAGAGCC CAAGGCAGAT 
GGCAGCTGGA GGGAAGGAGA CGAAGTCACA CTCATCTGCT CTGCCCGCGG CCATCCAGAC 
CCCAAACTCA GCTGGAGCCA ATTGGGGGGC AGCCCCGCAG AGCCAATCCC CGGACGGCAG 
GGTTGGGTGA GCAGCTCTCT GACCCTGAAA GTGACCAGCG CCCTGAGCCG CGATGGCATC 
TCCTGTGAAG CCTCCAACCC CCACGGGAAC AAGCGCCATG TCTTCCACTT CGGCACCGTG 
AGCCCCCAGA CCTCCCAGGC TGGAGTGGCC GTCATGGCCG TGGCCGTCAG CGTGGGCCTC 
CTGCTCCTCG TCGTTGCTGT CTTCTACTGC GTGAGACGCA AAGGGGGCCC CTGCTGCCGC 
CAGCGGCGGG AGAAGGGGGC TCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013275.1
CDS	67..1833
Translation	MEPPDAPAQARGAPRLLLLAVLLAAHPDAQAEVRLSVPPLVEVMRGKSVILDCTPTGTHDHYMLEWFLTDRSGARPRLASAEMQGSELQVTMHDTRGRSPPYQLDSQGRLVLAEAQVGDERDYVCVVRAGAAGTAEATARLNVFAKPEATEVSPNKGTLSVMEDSAQEIATCNSRNGNPAPKITWYRNGQRLEVPVEMNPEGYMTSRTVREASGLLSLTSTLYLRLRKDDRDASFHCAAHYSLPEGRHGRLDSPTFHLTLHYPTEHVQFWVGSPSTPAGWVREGDTVQLLCRGDGSPSPEYTLFRLQDEQEEVLNVNLEGNLTLEGVTRGQSGTYGCRVEDYDAADDVQLSKTLELRVAYLDPLELSEGKVLSLPLNSSAVVNCSVHGLPTPALRWTKDSTPLGDGPMLSLSSITFDSNGTYVCEASLPTVPVLSRTQNFTLLVQGSPELKTAEIEPKADGSWREGDEVTLICSARGHPDPKLSWSQLGGSPAEPIPGRQGWVSSSLTLKVTSALSRDGISCEASNPHGNKRHVFHFGTVSPQTSQAGVAVMAVAVSVGLLLLVVAVFYCVRRKGGPCCRQRREKGAP

Target ORF information:

RefSeq Version	NM_001013257.2
Organism	Homo sapiens(human)
Definition	Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013257.2

ORF Insert Sequence:

ATGGAGCCCC CGGACGCACC GGCCCAGGCG CGCGGGGCCC CGCGGCTGCT GTTGCTCGCA 
GTCCTGCTGG CGGCGCACCC AGATGCCCAG GCGGAGGTGC GCTTGTCTGT ACCCCCGCTG 
GTGGAGGTGA TGCGAGGAAA GTCTGTCATT CTGGACTGCA CCCCTACGGG AACCCACGAC 
CATTATATGC TGGAATGGTT CCTTACCGAC CGCTCGGGAG CTCGCCCCCG CCTAGCCTCG 
GCTGAGATGC AGGGCTCTGA GCTCCAGGTC ACAATGCACG ACACCCGGGG CCGCAGTCCC 
CCATACCAGC TGGACTCCCA GGGGCGCCTG GTGCTGGCTG AGGCCCAGGT GGGCGACGAG 
CGAGACTACG TGTGCGTGGT GAGGGCAGGG GCGGCAGGCA CTGCTGAGGC CACTGCGCGG 
CTCAACGTGT TTGCAAAGCC AGAGGCCACT GAGGTCTCCC CCAACAAAGG GACACTGTCT 
GTGATGGAGG ACTCTGCCCA GGAGATCGCC ACCTGCAACA GCCGGAACGG GAACCCGGCC 
CCCAAGATCA CGTGGTATCG CAACGGGCAG CGCCTGGAGG TGCCCGTAGA GATGAACCCA 
GAGGGCTACA TGACCAGCCG CACGGTCCGG GAGGCCTCGG GCCTGCTCTC CCTCACCAGC 
ACCCTCTACC TGCGGCTCCG CAAGGATGAC CGAGACGCCA GCTTCCACTG CGCCGCCCAC 
TACAGCCTGC CCGAGGGCCG CCACGGCCGC CTGGACAGCC CCACCTTCCA CCTCACCCTG 
CACTATCCCA CGGAGCACGT GCAGTTCTGG GTGGGCAGCC CGTCCACCCC AGCAGGCTGG 
GTACGCGAGG GTGACACTGT CCAGCTGCTC TGCCGGGGGG ACGGCAGCCC CAGCCCGGAG 
TATACGCTTT TCCGCCTTCA GGATGAGCAG GAGGAAGTGC TGAATGTGAA TCTCGAGGGG 
AACTTGACCC TGGAGGGAGT GACCCGGGGC CAGAGCGGGA CCTATGGCTG CAGAGTGGAG 
GATTACGACG CGGCAGATGA CGTGCAGCTC TCCAAGACGC TGGAGCTGCG CGTGGCCTAT 
CTGGACCCCC TGGAGCTCAG CGAGGGGAAG GTGCTTTCCT TACCTCTAAA CAGCAGTGCA 
GTCGTGAACT GCTCCGTGCA CGGCCTGCCC ACCCCTGCCC TACGCTGGAC CAAGGACTCC 
ACTCCCCTGG GCGATGGCCC CATGCTGTCG CTCAGTTCTA TCACCTTCGA TTCCAATGGC 
ACCTACGTAT GTGAGGCCTC CCTGCCCACA GTCCCGGTCC TCAGCCGCAC CCAGAACTTC 
ACGCTGCTGG TCCAAGGCTC GCCAGAGCTA AAGACAGCGG AAATAGAGCC CAAGGCAGAT 
GGCAGCTGGA GGGAAGGAGA CGAAGTCACA CTCATCTGCT CTGCCCGCGG CCATCCAGAC 
CCCAAACTCA GCTGGAGCCA ATTGGGGGGC AGCCCCGCAG AGCCAATCCC CGGACGGCAG 
GGTTGGGTGA GCAGCTCTCT GACCCTGAAA GTGACCAGCG CCCTGAGCCG CGATGGCATC 
TCCTGTGAAG CCTCCAACCC CCACGGGAAC AAGCGCCATG TCTTCCACTT CGGCACCGTG 
AGCCCCCAGA CCTCCCAGGC TGGAGTGGCC GTCATGGCCG TGGCCGTCAG CGTGGGCCTC 
CTGCTCCTCG TCGTTGCTGT CTTCTACTGC GTGAGACGCA AAGGGGGCCC CTGCTGCCGC 
CAGCGGCGGG AGAAGGGGGC TCCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAM ( NM_005581.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005572.2 Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20355
Clone ID Related Accession (Same CDS sequence)	NM_005581.4 , NM_005581.5
Accession Version	NM_005581.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	basal cell adhesion molecule isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP352029.1, BC050450.1 and BM985314.1. This sequence is a reference standard in the RefSeqGene project. On May 6, 2012 this sequence version replaced NM_005581.3. Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC050450.1, X83425.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCC CGGACGCACC GGCCCAGGCG CGCGGGGCCC CGCGGCTGCT GTTGCTCGCA 
GTCCTGCTGG CGGCGCACCC AGATGCCCAG GCGGAGGTGC GCTTGTCTGT ACCCCCGCTG 
GTGGAGGTGA TGCGAGGAAA GTCTGTCATT CTGGACTGCA CCCCTACGGG AACCCACGAC 
CATTATATGC TGGAATGGTT CCTTACCGAC CGCTCGGGAG CTCGCCCCCG CCTAGCCTCG 
GCTGAGATGC AGGGCTCTGA GCTCCAGGTC ACAATGCACG ACACCCGGGG CCGCAGTCCC 
CCATACCAGC TGGACTCCCA GGGGCGCCTG GTGCTGGCTG AGGCCCAGGT GGGCGACGAG 
CGAGACTACG TGTGCGTGGT GAGGGCAGGG GCGGCAGGCA CTGCTGAGGC CACTGCGCGG 
CTCAACGTGT TTGCAAAGCC AGAGGCCACT GAGGTCTCCC CCAACAAAGG GACACTGTCT 
GTGATGGAGG ACTCTGCCCA GGAGATCGCC ACCTGCAACA GCCGGAACGG GAACCCGGCC 
CCCAAGATCA CGTGGTATCG CAACGGGCAG CGCCTGGAGG TGCCCGTAGA GATGAACCCA 
GAGGGCTACA TGACCAGCCG CACGGTCCGG GAGGCCTCGG GCCTGCTCTC CCTCACCAGC 
ACCCTCTACC TGCGGCTCCG CAAGGATGAC CGAGACGCCA GCTTCCACTG CGCCGCCCAC 
TACAGCCTGC CCGAGGGCCG CCACGGCCGC CTGGACAGCC CCACCTTCCA CCTCACCCTG 
CACTATCCCA CGGAGCACGT GCAGTTCTGG GTGGGCAGCC CGTCCACCCC AGCAGGCTGG 
GTACGCGAGG GTGACACTGT CCAGCTGCTC TGCCGGGGGG ACGGCAGCCC CAGCCCGGAG 
TATACGCTTT TCCGCCTTCA GGATGAGCAG GAGGAAGTGC TGAATGTGAA TCTCGAGGGG 
AACTTGACCC TGGAGGGAGT GACCCGGGGC CAGAGCGGGA CCTATGGCTG CAGAGTGGAG 
GATTACGACG CGGCAGATGA CGTGCAGCTC TCCAAGACGC TGGAGCTGCG CGTGGCCTAT 
CTGGACCCCC TGGAGCTCAG CGAGGGGAAG GTGCTTTCCT TACCTCTAAA CAGCAGTGCA 
GTCGTGAACT GCTCCGTGCA CGGCCTGCCC ACCCCTGCCC TACGCTGGAC CAAGGACTCC 
ACTCCCCTGG GCGATGGCCC CATGCTGTCG CTCAGTTCTA TCACCTTCGA TTCCAATGGC 
ACCTACGTAT GTGAGGCCTC CCTGCCCACA GTCCCGGTCC TCAGCCGCAC CCAGAACTTC 
ACGCTGCTGG TCCAAGGCTC GCCAGAGCTA AAGACAGCGG AAATAGAGCC CAAGGCAGAT 
GGCAGCTGGA GGGAAGGAGA CGAAGTCACA CTCATCTGCT CTGCCCGCGG CCATCCAGAC 
CCCAAACTCA GCTGGAGCCA ATTGGGGGGC AGCCCCGCAG AGCCAATCCC CGGACGGCAG 
GGTTGGGTGA GCAGCTCTCT GACCCTGAAA GTGACCAGCG CCCTGAGCCG CGATGGCATC 
TCCTGTGAAG CCTCCAACCC CCACGGGAAC AAGCGCCATG TCTTCCACTT CGGCACCGTG 
AGCCCCCAGA CCTCCCAGGC TGGAGTGGCC GTCATGGCCG TGGCCGTCAG CGTGGGCCTC 
CTGCTCCTCG TCGTTGCTGT CTTCTACTGC GTGAGACGCA AAGGGGGCCC CTGCTGCCGC 
CAGCGGCGGG AGAAGGGGGC TCCGCCGCCA GGGGAGCCAG GGCTGAGCCA CTCGGGGTCG 
GAGCAACCAG AGCAGACCGG CCTTCTCATG GGAGGTGCCT CCGGAGGAGC CAGGGGTGGC 
AGCGGGGGCT TCGGAGACGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005572.2
CDS	67..1953
Translation	MEPPDAPAQARGAPRLLLLAVLLAAHPDAQAEVRLSVPPLVEVMRGKSVILDCTPTGTHDHYMLEWFLTDRSGARPRLASAEMQGSELQVTMHDTRGRSPPYQLDSQGRLVLAEAQVGDERDYVCVVRAGAAGTAEATARLNVFAKPEATEVSPNKGTLSVMEDSAQEIATCNSRNGNPAPKITWYRNGQRLEVPVEMNPEGYMTSRTVREASGLLSLTSTLYLRLRKDDRDASFHCAAHYSLPEGRHGRLDSPTFHLTLHYPTEHVQFWVGSPSTPAGWVREGDTVQLLCRGDGSPSPEYTLFRLQDEQEEVLNVNLEGNLTLEGVTRGQSGTYGCRVEDYDAADDVQLSKTLELRVAYLDPLELSEGKVLSLPLNSSAVVNCSVHGLPTPALRWTKDSTPLGDGPMLSLSSITFDSNGTYVCEASLPTVPVLSRTQNFTLLVQGSPELKTAEIEPKADGSWREGDEVTLICSARGHPDPKLSWSQLGGSPAEPIPGRQGWVSSSLTLKVTSALSRDGISCEASNPHGNKRHVFHFGTVSPQTSQAGVAVMAVAVSVGLLLLVVAVFYCVRRKGGPCCRQRREKGAPPPGEPGLSHSGSEQPEQTGLLMGGASGGARGGSGGFGDEC

Target ORF information:

RefSeq Version	NM_005581.4
Organism	Homo sapiens(human)
Definition	Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005581.4

ORF Insert Sequence:

ATGGAGCCCC CGGACGCACC GGCCCAGGCG CGCGGGGCCC CGCGGCTGCT GTTGCTCGCA 
GTCCTGCTGG CGGCGCACCC AGATGCCCAG GCGGAGGTGC GCTTGTCTGT ACCCCCGCTG 
GTGGAGGTGA TGCGAGGAAA GTCTGTCATT CTGGACTGCA CCCCTACGGG AACCCACGAC 
CATTATATGC TGGAATGGTT CCTTACCGAC CGCTCGGGAG CTCGCCCCCG CCTAGCCTCG 
GCTGAGATGC AGGGCTCTGA GCTCCAGGTC ACAATGCACG ACACCCGGGG CCGCAGTCCC 
CCATACCAGC TGGACTCCCA GGGGCGCCTG GTGCTGGCTG AGGCCCAGGT GGGCGACGAG 
CGAGACTACG TGTGCGTGGT GAGGGCAGGG GCGGCAGGCA CTGCTGAGGC CACTGCGCGG 
CTCAACGTGT TTGCAAAGCC AGAGGCCACT GAGGTCTCCC CCAACAAAGG GACACTGTCT 
GTGATGGAGG ACTCTGCCCA GGAGATCGCC ACCTGCAACA GCCGGAACGG GAACCCGGCC 
CCCAAGATCA CGTGGTATCG CAACGGGCAG CGCCTGGAGG TGCCCGTAGA GATGAACCCA 
GAGGGCTACA TGACCAGCCG CACGGTCCGG GAGGCCTCGG GCCTGCTCTC CCTCACCAGC 
ACCCTCTACC TGCGGCTCCG CAAGGATGAC CGAGACGCCA GCTTCCACTG CGCCGCCCAC 
TACAGCCTGC CCGAGGGCCG CCACGGCCGC CTGGACAGCC CCACCTTCCA CCTCACCCTG 
CACTATCCCA CGGAGCACGT GCAGTTCTGG GTGGGCAGCC CGTCCACCCC AGCAGGCTGG 
GTACGCGAGG GTGACACTGT CCAGCTGCTC TGCCGGGGGG ACGGCAGCCC CAGCCCGGAG 
TATACGCTTT TCCGCCTTCA GGATGAGCAG GAGGAAGTGC TGAATGTGAA TCTCGAGGGG 
AACTTGACCC TGGAGGGAGT GACCCGGGGC CAGAGCGGGA CCTATGGCTG CAGAGTGGAG 
GATTACGACG CGGCAGATGA CGTGCAGCTC TCCAAGACGC TGGAGCTGCG CGTGGCCTAT 
CTGGACCCCC TGGAGCTCAG CGAGGGGAAG GTGCTTTCCT TACCTCTAAA CAGCAGTGCA 
GTCGTGAACT GCTCCGTGCA CGGCCTGCCC ACCCCTGCCC TACGCTGGAC CAAGGACTCC 
ACTCCCCTGG GCGATGGCCC CATGCTGTCG CTCAGTTCTA TCACCTTCGA TTCCAATGGC 
ACCTACGTAT GTGAGGCCTC CCTGCCCACA GTCCCGGTCC TCAGCCGCAC CCAGAACTTC 
ACGCTGCTGG TCCAAGGCTC GCCAGAGCTA AAGACAGCGG AAATAGAGCC CAAGGCAGAT 
GGCAGCTGGA GGGAAGGAGA CGAAGTCACA CTCATCTGCT CTGCCCGCGG CCATCCAGAC 
CCCAAACTCA GCTGGAGCCA ATTGGGGGGC AGCCCCGCAG AGCCAATCCC CGGACGGCAG 
GGTTGGGTGA GCAGCTCTCT GACCCTGAAA GTGACCAGCG CCCTGAGCCG CGATGGCATC 
TCCTGTGAAG CCTCCAACCC CCACGGGAAC AAGCGCCATG TCTTCCACTT CGGCACCGTG 
AGCCCCCAGA CCTCCCAGGC TGGAGTGGCC GTCATGGCCG TGGCCGTCAG CGTGGGCCTC 
CTGCTCCTCG TCGTTGCTGT CTTCTACTGC GTGAGACGCA AAGGGGGCCC CTGCTGCCGC 
CAGCGGCGGG AGAAGGGGGC TCCGCCGCCA GGGGAGCCAG GGCTGAGCCA CTCGGGGTCG 
GAGCAACCAG AGCAGACCGG CCTTCTCATG GGAGGTGCCT CCGGAGGAGC CAGGGGTGGC 
AGCGGGGGCT TCGGAGACGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

BCAM ( NM_005581.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005572.2 Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 1, mRNA.

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CloneID	OHu20355
Clone ID Related Accession (Same CDS sequence)	NM_005581.4 , NM_005581.5
Accession Version	NM_005581.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	basal cell adhesion molecule isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP352029.1, BC050450.1 and BM985314.1. On Aug 31, 2019 this sequence version replaced NM_005581.4. Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X83425.1, BC050450.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCCCC CGGACGCACC GGCCCAGGCG CGCGGGGCCC CGCGGCTGCT GTTGCTCGCA 
GTCCTGCTGG CGGCGCACCC AGATGCCCAG GCGGAGGTGC GCTTGTCTGT ACCCCCGCTG 
GTGGAGGTGA TGCGAGGAAA GTCTGTCATT CTGGACTGCA CCCCTACGGG AACCCACGAC 
CATTATATGC TGGAATGGTT CCTTACCGAC CGCTCGGGAG CTCGCCCCCG CCTAGCCTCG 
GCTGAGATGC AGGGCTCTGA GCTCCAGGTC ACAATGCACG ACACCCGGGG CCGCAGTCCC 
CCATACCAGC TGGACTCCCA GGGGCGCCTG GTGCTGGCTG AGGCCCAGGT GGGCGACGAG 
CGAGACTACG TGTGCGTGGT GAGGGCAGGG GCGGCAGGCA CTGCTGAGGC CACTGCGCGG 
CTCAACGTGT TTGCAAAGCC AGAGGCCACT GAGGTCTCCC CCAACAAAGG GACACTGTCT 
GTGATGGAGG ACTCTGCCCA GGAGATCGCC ACCTGCAACA GCCGGAACGG GAACCCGGCC 
CCCAAGATCA CGTGGTATCG CAACGGGCAG CGCCTGGAGG TGCCCGTAGA GATGAACCCA 
GAGGGCTACA TGACCAGCCG CACGGTCCGG GAGGCCTCGG GCCTGCTCTC CCTCACCAGC 
ACCCTCTACC TGCGGCTCCG CAAGGATGAC CGAGACGCCA GCTTCCACTG CGCCGCCCAC 
TACAGCCTGC CCGAGGGCCG CCACGGCCGC CTGGACAGCC CCACCTTCCA CCTCACCCTG 
CACTATCCCA CGGAGCACGT GCAGTTCTGG GTGGGCAGCC CGTCCACCCC AGCAGGCTGG 
GTACGCGAGG GTGACACTGT CCAGCTGCTC TGCCGGGGGG ACGGCAGCCC CAGCCCGGAG 
TATACGCTTT TCCGCCTTCA GGATGAGCAG GAGGAAGTGC TGAATGTGAA TCTCGAGGGG 
AACTTGACCC TGGAGGGAGT GACCCGGGGC CAGAGCGGGA CCTATGGCTG CAGAGTGGAG 
GATTACGACG CGGCAGATGA CGTGCAGCTC TCCAAGACGC TGGAGCTGCG CGTGGCCTAT 
CTGGACCCCC TGGAGCTCAG CGAGGGGAAG GTGCTTTCCT TACCTCTAAA CAGCAGTGCA 
GTCGTGAACT GCTCCGTGCA CGGCCTGCCC ACCCCTGCCC TACGCTGGAC CAAGGACTCC 
ACTCCCCTGG GCGATGGCCC CATGCTGTCG CTCAGTTCTA TCACCTTCGA TTCCAATGGC 
ACCTACGTAT GTGAGGCCTC CCTGCCCACA GTCCCGGTCC TCAGCCGCAC CCAGAACTTC 
ACGCTGCTGG TCCAAGGCTC GCCAGAGCTA AAGACAGCGG AAATAGAGCC CAAGGCAGAT 
GGCAGCTGGA GGGAAGGAGA CGAAGTCACA CTCATCTGCT CTGCCCGCGG CCATCCAGAC 
CCCAAACTCA GCTGGAGCCA ATTGGGGGGC AGCCCCGCAG AGCCAATCCC CGGACGGCAG 
GGTTGGGTGA GCAGCTCTCT GACCCTGAAA GTGACCAGCG CCCTGAGCCG CGATGGCATC 
TCCTGTGAAG CCTCCAACCC CCACGGGAAC AAGCGCCATG TCTTCCACTT CGGCACCGTG 
AGCCCCCAGA CCTCCCAGGC TGGAGTGGCC GTCATGGCCG TGGCCGTCAG CGTGGGCCTC 
CTGCTCCTCG TCGTTGCTGT CTTCTACTGC GTGAGACGCA AAGGGGGCCC CTGCTGCCGC 
CAGCGGCGGG AGAAGGGGGC TCCGCCGCCA GGGGAGCCAG GGCTGAGCCA CTCGGGGTCG 
GAGCAACCAG AGCAGACCGG CCTTCTCATG GGAGGTGCCT CCGGAGGAGC CAGGGGTGGC 
AGCGGGGGCT TCGGAGACGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005572.2
CDS	23..1909
Translation	MEPPDAPAQARGAPRLLLLAVLLAAHPDAQAEVRLSVPPLVEVMRGKSVILDCTPTGTHDHYMLEWFLTDRSGARPRLASAEMQGSELQVTMHDTRGRSPPYQLDSQGRLVLAEAQVGDERDYVCVVRAGAAGTAEATARLNVFAKPEATEVSPNKGTLSVMEDSAQEIATCNSRNGNPAPKITWYRNGQRLEVPVEMNPEGYMTSRTVREASGLLSLTSTLYLRLRKDDRDASFHCAAHYSLPEGRHGRLDSPTFHLTLHYPTEHVQFWVGSPSTPAGWVREGDTVQLLCRGDGSPSPEYTLFRLQDEQEEVLNVNLEGNLTLEGVTRGQSGTYGCRVEDYDAADDVQLSKTLELRVAYLDPLELSEGKVLSLPLNSSAVVNCSVHGLPTPALRWTKDSTPLGDGPMLSLSSITFDSNGTYVCEASLPTVPVLSRTQNFTLLVQGSPELKTAEIEPKADGSWREGDEVTLICSARGHPDPKLSWSQLGGSPAEPIPGRQGWVSSSLTLKVTSALSRDGISCEASNPHGNKRHVFHFGTVSPQTSQAGVAVMAVAVSVGLLLLVVAVFYCVRRKGGPCCRQRREKGAPPPGEPGLSHSGSEQPEQTGLLMGGASGGARGGSGGFGDEC

Target ORF information:

RefSeq Version	NM_005581.5
Organism	Homo sapiens(human)
Definition	Homo sapiens basal cell adhesion molecule (Lutheran blood group) (BCAM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005581.5

ORF Insert Sequence:

ATGGAGCCCC CGGACGCACC GGCCCAGGCG CGCGGGGCCC CGCGGCTGCT GTTGCTCGCA 
GTCCTGCTGG CGGCGCACCC AGATGCCCAG GCGGAGGTGC GCTTGTCTGT ACCCCCGCTG 
GTGGAGGTGA TGCGAGGAAA GTCTGTCATT CTGGACTGCA CCCCTACGGG AACCCACGAC 
CATTATATGC TGGAATGGTT CCTTACCGAC CGCTCGGGAG CTCGCCCCCG CCTAGCCTCG 
GCTGAGATGC AGGGCTCTGA GCTCCAGGTC ACAATGCACG ACACCCGGGG CCGCAGTCCC 
CCATACCAGC TGGACTCCCA GGGGCGCCTG GTGCTGGCTG AGGCCCAGGT GGGCGACGAG 
CGAGACTACG TGTGCGTGGT GAGGGCAGGG GCGGCAGGCA CTGCTGAGGC CACTGCGCGG 
CTCAACGTGT TTGCAAAGCC AGAGGCCACT GAGGTCTCCC CCAACAAAGG GACACTGTCT 
GTGATGGAGG ACTCTGCCCA GGAGATCGCC ACCTGCAACA GCCGGAACGG GAACCCGGCC 
CCCAAGATCA CGTGGTATCG CAACGGGCAG CGCCTGGAGG TGCCCGTAGA GATGAACCCA 
GAGGGCTACA TGACCAGCCG CACGGTCCGG GAGGCCTCGG GCCTGCTCTC CCTCACCAGC 
ACCCTCTACC TGCGGCTCCG CAAGGATGAC CGAGACGCCA GCTTCCACTG CGCCGCCCAC 
TACAGCCTGC CCGAGGGCCG CCACGGCCGC CTGGACAGCC CCACCTTCCA CCTCACCCTG 
CACTATCCCA CGGAGCACGT GCAGTTCTGG GTGGGCAGCC CGTCCACCCC AGCAGGCTGG 
GTACGCGAGG GTGACACTGT CCAGCTGCTC TGCCGGGGGG ACGGCAGCCC CAGCCCGGAG 
TATACGCTTT TCCGCCTTCA GGATGAGCAG GAGGAAGTGC TGAATGTGAA TCTCGAGGGG 
AACTTGACCC TGGAGGGAGT GACCCGGGGC CAGAGCGGGA CCTATGGCTG CAGAGTGGAG 
GATTACGACG CGGCAGATGA CGTGCAGCTC TCCAAGACGC TGGAGCTGCG CGTGGCCTAT 
CTGGACCCCC TGGAGCTCAG CGAGGGGAAG GTGCTTTCCT TACCTCTAAA CAGCAGTGCA 
GTCGTGAACT GCTCCGTGCA CGGCCTGCCC ACCCCTGCCC TACGCTGGAC CAAGGACTCC 
ACTCCCCTGG GCGATGGCCC CATGCTGTCG CTCAGTTCTA TCACCTTCGA TTCCAATGGC 
ACCTACGTAT GTGAGGCCTC CCTGCCCACA GTCCCGGTCC TCAGCCGCAC CCAGAACTTC 
ACGCTGCTGG TCCAAGGCTC GCCAGAGCTA AAGACAGCGG AAATAGAGCC CAAGGCAGAT 
GGCAGCTGGA GGGAAGGAGA CGAAGTCACA CTCATCTGCT CTGCCCGCGG CCATCCAGAC 
CCCAAACTCA GCTGGAGCCA ATTGGGGGGC AGCCCCGCAG AGCCAATCCC CGGACGGCAG 
GGTTGGGTGA GCAGCTCTCT GACCCTGAAA GTGACCAGCG CCCTGAGCCG CGATGGCATC 
TCCTGTGAAG CCTCCAACCC CCACGGGAAC AAGCGCCATG TCTTCCACTT CGGCACCGTG 
AGCCCCCAGA CCTCCCAGGC TGGAGTGGCC GTCATGGCCG TGGCCGTCAG CGTGGGCCTC 
CTGCTCCTCG TCGTTGCTGT CTTCTACTGC GTGAGACGCA AAGGGGGCCC CTGCTGCCGC 
CAGCGGCGGG AGAAGGGGGC TCCGCCGCCA GGGGAGCCAG GGCTGAGCCA CTCGGGGTCG 
GAGCAACCAG AGCAGACCGG CCTTCTCATG GGAGGTGCCT CCGGAGGAGC CAGGGGTGGC 
AGCGGGGGCT TCGGAGACGA GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Adhesive activity of Lu glycoproteins is regulated by interaction with spectrin.
Blood112(13)5212-8(2008 Dec)
An X,Gauthier E,Zhang X,Guo X,Anstee DJ,Mohandas N,Chasis JA

The Laminin 511/521-binding site on the Lutheran blood group glycoprotein is located at the flexible junction of Ig domains 2 and 3.
Blood110(9)3398-406(2007 Nov)
Mankelow TJ,Burton N,Stefansdottir FO,Spring FA,Parsons SF,Pedersen JS,Oliveira CL,Lammie D,Wess T,Mohandas N,Chasis JA,Brady RL,Anstee DJ

Characterization of the laminin binding domains of the Lutheran blood group glycoprotein.
The Journal of biological chemistry276(26)23757-62(2001 Jun)
El Nemer W,Gane P,Colin Y,D'Ambrosio AM,Callebaut I,Cartron JP,Van Kim CL

Lutheran blood group glycoprotein and its newly characterized mouse homologue specifically bind alpha5 chain-containing human laminin with high affinity.
Blood97(1)312-20(2001 Jan)
Parsons SF,Lee G,Spring FA,Willig TN,Peters LL,Gimm JA,Tanner MJ,Mohandas N,Anstee DJ,Chasis JA

The Lutheran blood group glycoproteins, the erythroid receptors for laminin, are adhesion molecules.
The Journal of biological chemistry273(27)16686-93(1998 Jul)
El Nemer W,Gane P,Colin Y,Bony V,Rahuel C,Galact?ros F,Cartron JP,Le Van Kim C

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BCAM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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