LTC4S cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LTC4S
Entrez Gene ID	4056
Full Name	leukotriene C4 synthase
General protein information	Preferred Names leukotriene C4 synthase Names leukotriene C4 synthase LTC4 synthase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q35.3
Summary	The MAPEG (Membrane Associated Proteins in Eicosanoid and Glutathione metabolism) family includes a number of human proteins, several of which are involved the production of leukotrienes. This gene encodes an enzyme that catalyzes the first step in the biosynthesis of cysteinyl leukotrienes, potent biological compounds derived from arachidonic acid. Leukotrienes have been implicated as mediators of anaphylaxis and inflammatory conditions such as human bronchial asthma. This protein localizes to the nuclear envelope and adjacent endoplasmic reticulum. [provided by RefSeq, Jul 2008].
Disorder MIM:	246530
Disorder Html:	Leukotriene C4 synthase deficiency, 614037 (1)

mRNA and Protein(s)

mRNA	Protein	Name
NM_145867.2	NP_665874.1	leukotriene C4 synthase
NM_145867.1	NP_665874.1	leukotriene C4 synthase

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	mgst2	NP_001106565.1
Pan troglodytes (chimpanzee)	LTC4S	XP_001153488.2
	LTC4S	XP_001101985.1
Canis lupus familiaris (dog)	LTC4S	XP_849032.2
Bos taurus (cattle)	LTC4S	NP_001039563.2
Gallus gallus (chicken)	LOC770619	XP_001233958.3
Mus musculus (house mouse)	Ltc4s	NP_032547.1
Homo sapiens (human)	LTC4S	NP_665874.1
Rattus norvegicus (Norway rat)	Ltc4s	NP_446091.2

Gene Ontology
Bibliography

Related articles in PubMed

Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs Catalytic Activity.
Ahmad S, Ytterberg AJ, Thulasingam M, Tholander F, Bergman T, Zubarev R, Wetterholm A, Rinaldo-Matthis A, Haeggstr?m JZ
The Journal of biological chemistry291(35)18410-8(2016 08)

Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.
Niegowski D, Kleinschmidt T, Olsson U, Ahmad S, Rinaldo-Matthis A, Haeggstr?m JZ
The Journal of biological chemistry289(8)5199-207(2014 Feb)

Lack of effect of common single nucleotide polymorphisms in leukotriene pathway genes on platelet reactivity in patients with diabetes.
Rosiak M, Postula M, Kaplon-Cieslicka A, Kondracka A, Trzepla E, Zaremba M, Filipiak KJ, Kosior DA, Czlonkowski A, Opolski G, Janicki PK
Molecular medicine reports8(3)853-60(2013 Sep)

Elevated levels of leukotriene C4 synthase mRNA distinguish a subpopulation of eosinophilic oesophagitis patients.
Lexmond WS, Pardo M, Rooney K, Goettel JA, Snapper SB, Yen EH, Dehlink E, Nurko S, Fiebiger E
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology43(8)902-13(2013 Aug)

Genetic variation in members of the leukotrienes biosynthesis pathway confers risk of ischemic stroke in Eastern Han Chinese.
Wang G, Zhang J, Sun H, Cao W, Zhang J, Wang Y, Xiao H
Prostaglandins, leukotrienes, and essential fatty acids87(6)169-75(2012 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

mutational and kinetic data, together with molecular simulations, suggest that phosphorylation of Ser(36) inhibits the catalytic function of LTC4S by interference with the catalytic machinery.
Title: Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs Catalytic Activity.

Biochemical and structural characterization of LTC4S mutants along with crystal structures of the wild type and mutated enzymes in complex with three product analogs, provide new insights to binding of substrates and product.
Title: Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.

The results of our study failed to confirm whether the selected variants in LTC4S gene within the LT metabolism pathway contribute to platelet reactivity in a diabetic population treated with ASA.
Title: Lack of effect of common single nucleotide polymorphisms in leukotriene pathway genes on platelet reactivity in patients with diabetes.

Elevated levels of leukotriene C4 synthase mRNA distinguish a subpopulation of eosinophilic oesophagitis patients.
Title: Elevated levels of leukotriene C4 synthase mRNA distinguish a subpopulation of eosinophilic oesophagitis patients.

genetic association studies in population of Han Chinese in Eastern China: Data suggest that an SNP in LTC4S (rs730012) is associated with risk of ischemic stroke; carriers of C allele of rs730012 in LTC4S are most susceptible to ischemic stroke.
Title: Genetic variation in members of the leukotrienes biosynthesis pathway confers risk of ischemic stroke in Eastern Han Chinese.

The following LTC4S gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LTC4S cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18702	NM_145867.2 Latest version!	Homo sapiens leukotriene C4 synthase (LTC4S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu18702	NM_145867.1	Homo sapiens leukotriene C4 synthase (LTC4S), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LTC4S ( NM_145867.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_665874.1 Homo sapiens leukotriene C4 synthase (LTC4S), mRNA.

Price & Availability↑

CloneID	OHu18702
Clone ID Related Accession (Same CDS sequence)	NM_145867.2 , NM_145867.1
Accession Version	NM_145867.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	leukotriene C4 synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008393.7. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_145867.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC029498.1, BI668417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000292596.15/ ENSP00000292596.10 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAAGGACG AGGTAGCTCT ACTGGCTGCT GTCACCCTCC TGGGAGTCCT GCTGCAAGCC 
TACTTCTCCC TGCAGGTGAT CTCGGCGCGC AGGGCCTTCC GCGTGTCGCC GCCGCTCACC 
ACCGGCCCAC CCGAGTTCGA GCGCGTCTAC CGAGCCCAGG TGAACTGCAG CGAGTACTTC 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TTCATGAAGG GGCGGCGGCC 
CTGTGCGGCC TGGTCTACCT GTTCGCGCGC CTCCGCTACT TCCAGGGCTA CGCGCGCTCC 
GCGCAGCTCA GGCTGGCACC GCTGTACGCG AGCGCGCGCG CCCTCTGGCT GCTGGTGGCG 
CTGGCTGCGC TCGGCCTGCT CGCCCACTTC CTCCCGGCCG CGCTGCGCGC CGCGCTCCTC 
GGACGGCTCC GGACGCTGCT GCCGTGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665874.1
CDS	96..548
Translation	MKDEVALLAAVTLLGVLLQAYFSLQVISARRAFRVSPPLTTGPPEFERVYRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLVYLFARLRYFQGYARSAQLRLAPLYASARALWLLVALAALGLLAHFLPAALRAALLGRLRTLLPWA

Target ORF information:

RefSeq Version	NM_145867.2
Organism	Homo sapiens(human)
Definition	Homo sapiens leukotriene C4 synthase (LTC4S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145867.2

ORF Insert Sequence:

ATGAAGGACG AGGTAGCTCT ACTGGCTGCT GTCACCCTCC TGGGAGTCCT GCTGCAAGCC 
TACTTCTCCC TGCAGGTGAT CTCGGCGCGC AGGGCCTTCC GCGTGTCGCC GCCGCTCACC 
ACCGGCCCAC CCGAGTTCGA GCGCGTCTAC CGAGCCCAGG TGAACTGCAG CGAGTACTTC 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TTCATGAAGG GGCGGCGGCC 
CTGTGCGGCC TGGTCTACCT GTTCGCGCGC CTCCGCTACT TCCAGGGCTA CGCGCGCTCC 
GCGCAGCTCA GGCTGGCACC GCTGTACGCG AGCGCGCGCG CCCTCTGGCT GCTGGTGGCG 
CTGGCTGCGC TCGGCCTGCT CGCCCACTTC CTCCCGGCCG CGCTGCGCGC CGCGCTCCTC 
GGACGGCTCC GGACGCTGCT GCCGTGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LTC4S ( NM_145867.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_665874.1 Homo sapiens leukotriene C4 synthase (LTC4S), mRNA.

Price & Availability↑

CloneID	OHu18702
Clone ID Related Accession (Same CDS sequence)	NM_145867.2 , NM_145867.1
Accession Version	NM_145867.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 453bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	leukotriene C4 synthase
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC029498.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BY995423.1, BI668417.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGAAGGACG AGGTAGCTCT ACTGGCTGCT GTCACCCTCC TGGGAGTCCT GCTGCAAGCC 
TACTTCTCCC TGCAGGTGAT CTCGGCGCGC AGGGCCTTCC GCGTGTCGCC GCCGCTCACC 
ACCGGCCCAC CCGAGTTCGA GCGCGTCTAC CGAGCCCAGG TGAACTGCAG CGAGTACTTC 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TTCATGAAGG GGCGGCGGCC 
CTGTGCGGCC TGGTCTACCT GTTCGCGCGC CTCCGCTACT TCCAGGGCTA CGCGCGCTCC 
GCGCAGCTCA GGCTGGCACC GCTGTACGCG AGCGCGCGCG CCCTCTGGCT GCTGGTGGCG 
CTGGCTGCGC TCGGCCTGCT CGCCCACTTC CTCCCGGCCG CGCTGCGCGC CGCGCTCCTC 
GGACGGCTCC GGACGCTGCT GCCGTGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665874.1
CDS	97..549
Translation	MKDEVALLAAVTLLGVLLQAYFSLQVISARRAFRVSPPLTTGPPEFERVYRAQVNCSEYFPLFLATLWVAGIFFHEGAAALCGLVYLFARLRYFQGYARSAQLRLAPLYASARALWLLVALAALGLLAHFLPAALRAALLGRLRTLLPWA

Target ORF information:

RefSeq Version	NM_145867.1
Organism	Homo sapiens(human)
Definition	Homo sapiens leukotriene C4 synthase (LTC4S), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145867.1

ORF Insert Sequence:

ATGAAGGACG AGGTAGCTCT ACTGGCTGCT GTCACCCTCC TGGGAGTCCT GCTGCAAGCC 
TACTTCTCCC TGCAGGTGAT CTCGGCGCGC AGGGCCTTCC GCGTGTCGCC GCCGCTCACC 
ACCGGCCCAC CCGAGTTCGA GCGCGTCTAC CGAGCCCAGG TGAACTGCAG CGAGTACTTC 
CCGCTGTTCC TCGCCACGCT CTGGGTCGCC GGCATCTTCT TTCATGAAGG GGCGGCGGCC 
CTGTGCGGCC TGGTCTACCT GTTCGCGCGC CTCCGCTACT TCCAGGGCTA CGCGCGCTCC 
GCGCAGCTCA GGCTGGCACC GCTGTACGCG AGCGCGCGCG CCCTCTGGCT GCTGGTGGCG 
CTGGCTGCGC TCGGCCTGCT CGCCCACTTC CTCCCGGCCG CGCTGCGCGC CGCGCTCCTC 
GGACGGCTCC GGACGCTGCT GCCGTGGGCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Phosphorylation of Leukotriene C4 Synthase at Serine 36 Impairs Catalytic Activity.
The Journal of biological chemistry291(35)18410-8(2016 08)
Ahmad S,Ytterberg AJ,Thulasingam M,Tholander F,Bergman T,Zubarev R,Wetterholm A,Rinaldo-Matthis A,Haeggstr?m JZ

Crystal structures of leukotriene C4 synthase in complex with product analogs: implications for the enzyme mechanism.
The Journal of biological chemistry289(8)5199-207(2014 Feb)
Niegowski D,Kleinschmidt T,Olsson U,Ahmad S,Rinaldo-Matthis A,Haeggstr?m JZ

Lack of effect of common single nucleotide polymorphisms in leukotriene pathway genes on platelet reactivity in patients with diabetes.
Molecular medicine reports8(3)853-60(2013 Sep)
Rosiak M,Postula M,Kaplon-Cieslicka A,Kondracka A,Trzepla E,Zaremba M,Filipiak KJ,Kosior DA,Czlonkowski A,Opolski G,Janicki PK

Elevated levels of leukotriene C4 synthase mRNA distinguish a subpopulation of eosinophilic oesophagitis patients.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology43(8)902-13(2013 Aug)
Lexmond WS,Pardo M,Rooney K,Goettel JA,Snapper SB,Yen EH,Dehlink E,Nurko S,Fiebiger E

Genetic variation in members of the leukotrienes biosynthesis pathway confers risk of ischemic stroke in Eastern Han Chinese.
Prostaglandins, leukotrienes, and essential fatty acids87(6)169-75(2012 Dec)
Wang G,Zhang J,Sun H,Cao W,Zhang J,Wang Y,Xiao H

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LTC4S cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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