LIMK2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIMK2
Entrez Gene ID	3985
Full Name	LIM domain kinase 2
General protein information	Preferred Names LIM domain kinase 2 Names LIM domain kinase 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 22 Map Location: 22q12.2
Summary	There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	601988

mRNA and Protein(s)

mRNA	Protein	Name
NM_005569.4	NP_005560.1	LIM domain kinase 2 isoform 2a
NM_001031801.2	NP_001026971.1	LIM domain kinase 2 isoform 1
NM_005569.3	NP_005560.1	LIM domain kinase 2 isoform 2a
NM_016733.2	NP_057952.1	LIM domain kinase 2 isoform 2b
NM_016733.3	NP_057952.1	LIM domain kinase 2 isoform 2b
NM_001031801.1	NP_001026971.1	LIM domain kinase 2 isoform 1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Limk2	NP_034848.1
Pan troglodytes (chimpanzee)	LIMK2	XP_003317251.1
Canis lupus familiaris (dog)	LIMK2	XP_852696.1
Rattus norvegicus (Norway rat)	Limk2	NP_077049.2
Bos taurus (cattle)	LIMK2	NP_001033187.1
Gallus gallus (chicken)	LIMK2	NP_990446.1
Xenopus tropicalis (tropical clawed frog)	limk2	NP_001135686.1
Danio rerio (zebrafish)	limk2	NP_001002651.1
Homo sapiens (human)	LIMK2	NP_005560.1

Gene Ontology
Bibliography

Related articles in PubMed

Single Nucleotide Polymorphisms of Paclitaxel-induced Peripheral Sensory Neuropathy in Chinese Han Population.
Dou XL, Mai YL, Sun Z, Wang YY, Shao YJ, Cheng YJ, Zhou N, Luo F, Zhang B, Bai CM, Ma SQ
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae39(5)593-601(2017 10)

The role of the IL-8 signaling pathway in the infiltration of granulocytes into the livers of patients with alcoholic hepatitis.
French SW, Mendoza AS, Afifiyan N, Tillman B, Vitocruz E, French BA
Experimental and molecular pathology103(2)137-140(2017 10)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High LIMK2 expression is associated with alcoholic hepatitis.
Title: The role of the IL-8 signaling pathway in the infiltration of granulocytes into the livers of patients with alcoholic hepatitis.

these results suggested that TUG1 mediates cell growth and chemoresistance of SCLC by regulating LIMK2b via EZH2.
Title: Long non-coding RNA TUG1 is involved in cell growth and chemoresistance of small cell lung cancer by regulating LIMK2b via EZH2.

We observed marked increases in LIM kinase 2 (LIMK2) and cofilin 1 (CFL1) gene expressions in metabolic syndrome patients.
Title: Evidence for elevated (LIMK2 and CFL1) and suppressed (ICAM1, EZR, MAP2K2, and NOS3) gene expressions in metabolic syndrome.

LIMK2 (rs149034313) is associated with Behcet's disease. The LIMK2 association is a missense variant with predicted protein damage that may influence functional interactions with proteins involved in cytoskeletal regulation by Rho GTPase, inflammation mediated by chemokine and cytokine signaling pathways, T cell activation, and angiogenesis.
Title: Whole Exome Sequencing Identifies Rare Protein-Coding Variants in Beh?et's Disease.

In turn, LIMK1 and LIMK2 are required for MT1-MMP-dependent matrix degradation and cell invasion in a three-dimensional type I collagen environment.
Title: LIMK Regulates Tumor-Cell Invasion and Matrix Degradation Through Tyrosine Phosphorylation of MT1-MMP.

The following LIMK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIMK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25805	NM_001031801.2 Latest version!	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu25734	NM_016733.3 Latest version!	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OHu25838	NM_005569.4 Latest version!	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$377.30 $539.00
View Old Accession Versions >>
OHu25838	NM_005569.3	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$377.30 $539.00
OHu25734	NM_016733.2	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$363.30 $519.00
OHu25805	NM_001031801.1	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIMK2 ( NM_001031801.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026971.1 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25805
Clone ID Related Accession (Same CDS sequence)	NM_001031801.2 , NM_001031801.1
Accession Version	NM_001031801.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	LIM domain kinase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU553494.1 and BC013051.1. On May 31, 2019 this sequence version replaced NM_001031801.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013051.1, SRR1803612.250640.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA GCCCCCCCCG GGGCCGCAGG AGAGGGCCCG 
GGCTGCGCGG ATGATGAGGG CCCAGTGAGG CGCCAAGGGA AGGTCACCAT CAAGTATGAC 
CCCAAGGAGC TACGGAAGCA CCTCAACCTA GAGGAGTGGA TCCTGGAGCA GCTCACGCGC 
CTCTACGACT GCCAGGAAGA GGAGATCTCA GAACTAGAGA TTGACGTGGA TGAGCTCCTG 
GACATGGAGA GTGACGATGC CTGGGCTTCC AGGGTCAAGG AGCTGCTGGT TGACTGTTAC 
AAACCCACAG AGGCCTTCAT CTCTGGCCTG CTGGACAAGA TCCGGGCCAT GCAGAAGCTG 
AGCACACCCC AGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026971.1
CDS	334..2394
Translation	MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYDPKELRKHLNLEEWILEQLTRLYDCQEEEISELEIDVDELLDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAMQKLSTPQKK

Target ORF information:

RefSeq Version	NM_001031801.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031801.2

ORF Insert Sequence:

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA GCCCCCCCCG GGGCCGCAGG AGAGGGCCCG 
GGCTGCGCGG ATGATGAGGG CCCAGTGAGG CGCCAAGGGA AGGTCACCAT CAAGTATGAC 
CCCAAGGAGC TACGGAAGCA CCTCAACCTA GAGGAGTGGA TCCTGGAGCA GCTCACGCGC 
CTCTACGACT GCCAGGAAGA GGAGATCTCA GAACTAGAGA TTGACGTGGA TGAGCTCCTG 
GACATGGAGA GTGACGATGC CTGGGCTTCC AGGGTCAAGG AGCTGCTGGT TGACTGTTAC 
AAACCCACAG AGGCCTTCAT CTCTGGCCTG CTGGACAAGA TCCGGGCCAT GCAGAAGCTG 
AGCACACCCC AGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK2 ( NM_001031801.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001026971.1 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25805
Clone ID Related Accession (Same CDS sequence)	NM_001031801.2 , NM_001031801.1
Accession Version	NM_001031801.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2061bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	LIM domain kinase 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU553494.1 and BC013051.1. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC013051.1, SRR1803615.19229.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA GCCCCCCCCG GGGCCGCAGG AGAGGGCCCG 
GGCTGCGCGG ATGATGAGGG CCCAGTGAGG CGCCAAGGGA AGGTCACCAT CAAGTATGAC 
CCCAAGGAGC TACGGAAGCA CCTCAACCTA GAGGAGTGGA TCCTGGAGCA GCTCACGCGC 
CTCTACGACT GCCAGGAAGA GGAGATCTCA GAACTAGAGA TTGACGTGGA TGAGCTCCTG 
GACATGGAGA GTGACGATGC CTGGGCTTCC AGGGTCAAGG AGCTGCTGGT TGACTGTTAC 
AAACCCACAG AGGCCTTCAT CTCTGGCCTG CTGGACAAGA TCCGGGCCAT GCAGAAGCTG 
AGCACACCCC AGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001026971.1
CDS	356..2416
Translation	MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRAPPGAAGEGPGCADDEGPVRRQGKVTIKYDPKELRKHLNLEEWILEQLTRLYDCQEEEISELEIDVDELLDMESDDAWASRVKELLVDCYKPTEAFISGLLDKIRAMQKLSTPQKK

Target ORF information:

RefSeq Version	NM_001031801.1
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001031801.1

ORF Insert Sequence:

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA GCCCCCCCCG GGGCCGCAGG AGAGGGCCCG 
GGCTGCGCGG ATGATGAGGG CCCAGTGAGG CGCCAAGGGA AGGTCACCAT CAAGTATGAC 
CCCAAGGAGC TACGGAAGCA CCTCAACCTA GAGGAGTGGA TCCTGGAGCA GCTCACGCGC 
CTCTACGACT GCCAGGAAGA GGAGATCTCA GAACTAGAGA TTGACGTGGA TGAGCTCCTG 
GACATGGAGA GTGACGATGC CTGGGCTTCC AGGGTCAAGG AGCTGCTGGT TGACTGTTAC 
AAACCCACAG AGGCCTTCAT CTCTGGCCTG CTGGACAAGA TCCGGGCCAT GCAGAAGCTG 
AGCACACCCC AGAAGAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK2 ( NM_016733.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_057952.1 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b, mRNA.

Price & Availability↑

CloneID	OHu25734
Clone ID Related Accession (Same CDS sequence)	NM_016733.2 , NM_016733.3
Accession Version	NM_016733.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	LIM domain kinase 2 isoform 2b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU553494.1, BC013051.1 and AC002073.1. On Aug 16, 2005 this sequence version replaced NM_016733.1. Transcript Variant: This variant (2b) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (2b) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.208285.1, SRR1803617.244252.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA CCAGCATTCT CGAAATTGGA GGACTCCTTT 
GAGGCCCTCT CCCTGTACCT GGGGGAGCTG GGCATCCCGC TGCCTGCAGA GCTGGAGGAG 
TTGGACCACA CTGTGAGCAT GCAGTACGGC CTGACCCGGG ACTCACCTCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057952.1
CDS	356..2209
Translation	MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

Target ORF information:

RefSeq Version	NM_016733.2
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016733.2

ORF Insert Sequence:

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA CCAGCATTCT CGAAATTGGA GGACTCCTTT 
GAGGCCCTCT CCCTGTACCT GGGGGAGCTG GGCATCCCGC TGCCTGCAGA GCTGGAGGAG 
TTGGACCACA CTGTGAGCAT GCAGTACGGC CTGACCCGGG ACTCACCTCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK2 ( NM_016733.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_057952.1 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b, mRNA.

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CloneID	OHu25734
Clone ID Related Accession (Same CDS sequence)	NM_016733.2 , NM_016733.3
Accession Version	NM_016733.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1854bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-02
Organism	Homo sapiens(human)
Product	LIM domain kinase 2 isoform 2b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BU553494.1, BC013051.1 and AC002073.1. On May 31, 2019 this sequence version replaced NM_016733.2. Transcript Variant: This variant (2b) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (2b) is shorter and has a distinct C-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1903954.1, SRR7346977.1613095.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA CCAGCATTCT CGAAATTGGA GGACTCCTTT 
GAGGCCCTCT CCCTGTACCT GGGGGAGCTG GGCATCCCGC TGCCTGCAGA GCTGGAGGAG 
TTGGACCACA CTGTGAGCAT GCAGTACGGC CTGACCCGGG ACTCACCTCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_057952.1
CDS	334..2187
Translation	MGSYLSVPAYFTSRDLFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

Target ORF information:

RefSeq Version	NM_016733.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_016733.3

ORF Insert Sequence:

ATGGGGAGTT ACTTGTCAGT CCCGGCTTAC TTCACCTCCA GAGACCTGTT TCGGTGTTCA 
GAATGCCAGG ATTCCCTCAC CAACTGGTAC TATGAGAAGG ATGGGAAGCT CTACTGCCCC 
AAGGACTACT GGGGGAAGTT TGGGGAGTTC TGTCATGGGT GCTCCCTGCT GATGACAGGG 
CCTTTTATGG TGGCTGGGGA GTTCAAGTAC CACCCAGAGT GCTTTGCCTG TATGAGCTGC 
AAGGTGATCA TTGAGGATGG GGATGCATAT GCACTGGTGC AGCATGCCAC CCTCTACTGT 
GGGAAGTGCC ACAATGAGGT GGTGCTGGCA CCCATGTTTG AGAGACTCTC CACAGAGTCT 
GTTCAGGAGC AGCTGCCCTA CTCTGTCACG CTCATCTCCA TGCCGGCCAC CACTGAAGGC 
AGGCGGGGCT TCTCCGTGTC CGTGGAGAGT GCCTGCTCCA ACTACGCCAC CACTGTGCAA 
GTGAAAGAGG TCAACCGGAT GCACATCAGT CCCAACAATC GAAACGCCAT CCACCCTGGG 
GACCGCATCC TGGAGATCAA TGGGACCCCC GTCCGCACAC TTCGAGTGGA GGAGGTGGAG 
GATGCAATTA GCCAGACGAG CCAGACACTT CAGCTGTTGA TTGAACATGA CCCCGTCTCC 
CAACGCCTGG ACCAGCTGCG GCTGGAGGCC CGGCTCGCTC CTCACATGCA GAATGCCGGA 
CACCCCCACG CCCTCAGCAC CCTGGACACC AAGGAGAATC TGGAGGGGAC ACTGAGGAGA 
CGTTCCCTAA GGCGCAGTAA CAGTATCTCC AAGTCCCCTG GCCCCAGCTC CCCAAAGGAG 
CCCCTGCTGT TCAGCCGTGA CATCAGCCGC TCAGAATCCC TTCGTTGTTC CAGCAGCTAT 
TCACAGCAGA TCTTCCGGCC CTGTGACCTA ATCCATGGGG AGGTCCTGGG GAAGGGCTTC 
TTTGGGCAGG CTATCAAGGT GACACACAAA GCCACGGGCA AAGTGATGGT CATGAAAGAG 
TTAATTCGAT GTGATGAGGA GACCCAGAAA ACTTTTCTGA CTGAGGTGAA AGTGATGCGC 
AGCCTGGACC ACCCCAATGT GCTCAAGTTC ATTGGTGTGC TGTACAAGGA TAAGAAGCTG 
AACCTCCTGA CAGAGTACAT TGAGGGGGGC ACACTGAAGG ACTTTCTGCG CAGTATGGAT 
CCGTTCCCCT GGCAGCAGAA GGTCAGGTTT GCCAAAGGAA TCGCCTCCGG AATGGCCTAT 
TTGCACTCTA TGTGCATCAT CCACCGGGAT CTGAACTCGC ACAACTGCCT CATCAAGTTG 
GACAAGACTG TGGTGGTGGC AGACTTTGGG CTGTCACGGC TCATAGTGGA AGAGAGGAAA 
AGGGCCCCCA TGGAGAAGGC CACCACCAAG AAACGCACCT TGCGCAAGAA CGACCGCAAG 
AAGCGCTACA CGGTGGTGGG AAACCCCTAC TGGATGGCCC CTGAGATGCT GAACGGAAAG 
AGCTATGATG AGACGGTGGA TATCTTCTCC TTTGGGATCG TTCTCTGTGA GATCATTGGG 
CAGGTGTATG CAGATCCTGA CTGCCTTCCC CGAACACTGG ACTTTGGCCT CAACGTGAAG 
CTTTTCTGGG AGAAGTTTGT TCCCACAGAT TGTCCCCCGG CCTTCTTCCC GCTGGCCGCC 
ATCTGCTGCA GACTGGAGCC TGAGAGCAGA CCAGCATTCT CGAAATTGGA GGACTCCTTT 
GAGGCCCTCT CCCTGTACCT GGGGGAGCTG GGCATCCCGC TGCCTGCAGA GCTGGAGGAG 
TTGGACCACA CTGTGAGCAT GCAGTACGGC CTGACCCGGG ACTCACCTCC CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK2 ( NM_005569.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005560.1 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a, mRNA.

Price & Availability↑

CloneID	OHu25838
Clone ID Related Accession (Same CDS sequence)	NM_005569.4 , NM_005569.3
Accession Version	NM_005569.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	LIM domain kinase 2 isoform 2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX385507.2, D45906.1, BC013051.1 and AC002073.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_005569.3. Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: D45906.1, SRR1803617.62527.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331728.9/ ENSP00000332687.4 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCCGCGC TGGCGGGTGA AGATGTCTGG AGGTGTCCAG GCTGTGGGGA CCACATTGCT 
CCAAGCCAGA TATGGTACAG GACTGTCAAC GAAACCTGGC ACGGCTCTTG CTTCCGGTGT 
TCAGAATGCC AGGATTCCCT CACCAACTGG TACTATGAGA AGGATGGGAA GCTCTACTGC 
CCCAAGGACT ACTGGGGGAA GTTTGGGGAG TTCTGTCATG GGTGCTCCCT GCTGATGACA 
GGGCCTTTTA TGGTGGCTGG GGAGTTCAAG TACCACCCAG AGTGCTTTGC CTGTATGAGC 
TGCAAGGTGA TCATTGAGGA TGGGGATGCA TATGCACTGG TGCAGCATGC CACCCTCTAC 
TGTGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAGAGACT CTCCACAGAG 
TCTGTTCAGG AGCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCGGC CACCACTGAA 
GGCAGGCGGG GCTTCTCCGT GTCCGTGGAG AGTGCCTGCT CCAACTACGC CACCACTGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGTCCCAACA ATCGAAACGC CATCCACCCT 
GGGGACCGCA TCCTGGAGAT CAATGGGACC CCCGTCCGCA CACTTCGAGT GGAGGAGGTG 
GAGGATGCAA TTAGCCAGAC GAGCCAGACA CTTCAGCTGT TGATTGAACA TGACCCCGTC 
TCCCAACGCC TGGACCAGCT GCGGCTGGAG GCCCGGCTCG CTCCTCACAT GCAGAATGCC 
GGACACCCCC ACGCCCTCAG CACCCTGGAC ACCAAGGAGA ATCTGGAGGG GACACTGAGG 
AGACGTTCCC TAAGGCGCAG TAACAGTATC TCCAAGTCCC CTGGCCCCAG CTCCCCAAAG 
GAGCCCCTGC TGTTCAGCCG TGACATCAGC CGCTCAGAAT CCCTTCGTTG TTCCAGCAGC 
TATTCACAGC AGATCTTCCG GCCCTGTGAC CTAATCCATG GGGAGGTCCT GGGGAAGGGC 
TTCTTTGGGC AGGCTATCAA GGTGACACAC AAAGCCACGG GCAAAGTGAT GGTCATGAAA 
GAGTTAATTC GATGTGATGA GGAGACCCAG AAAACTTTTC TGACTGAGGT GAAAGTGATG 
CGCAGCCTGG ACCACCCCAA TGTGCTCAAG TTCATTGGTG TGCTGTACAA GGATAAGAAG 
CTGAACCTCC TGACAGAGTA CATTGAGGGG GGCACACTGA AGGACTTTCT GCGCAGTATG 
GATCCGTTCC CCTGGCAGCA GAAGGTCAGG TTTGCCAAAG GAATCGCCTC CGGAATGGCC 
TATTTGCACT CTATGTGCAT CATCCACCGG GATCTGAACT CGCACAACTG CCTCATCAAG 
TTGGACAAGA CTGTGGTGGT GGCAGACTTT GGGCTGTCAC GGCTCATAGT GGAAGAGAGG 
AAAAGGGCCC CCATGGAGAA GGCCACCACC AAGAAACGCA CCTTGCGCAA GAACGACCGC 
AAGAAGCGCT ACACGGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAACGGA 
AAGAGCTATG ATGAGACGGT GGATATCTTC TCCTTTGGGA TCGTTCTCTG TGAGATCATT 
GGGCAGGTGT ATGCAGATCC TGACTGCCTT CCCCGAACAC TGGACTTTGG CCTCAACGTG 
AAGCTTTTCT GGGAGAAGTT TGTTCCCACA GATTGTCCCC CGGCCTTCTT CCCGCTGGCC 
GCCATCTGCT GCAGACTGGA GCCTGAGAGC AGACCAGCAT TCTCGAAATT GGAGGACTCC 
TTTGAGGCCC TCTCCCTGTA CCTGGGGGAG CTGGGCATCC CGCTGCCTGC AGAGCTGGAG 
GAGTTGGACC ACACTGTGAG CATGCAGTAC GGCCTGACCC GGGACTCACC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005560.1
CDS	112..2028
Translation	MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

Target ORF information:

RefSeq Version	NM_005569.4
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005569.4

ORF Insert Sequence:

ATGTCCGCGC TGGCGGGTGA AGATGTCTGG AGGTGTCCAG GCTGTGGGGA CCACATTGCT 
CCAAGCCAGA TATGGTACAG GACTGTCAAC GAAACCTGGC ACGGCTCTTG CTTCCGGTGT 
TCAGAATGCC AGGATTCCCT CACCAACTGG TACTATGAGA AGGATGGGAA GCTCTACTGC 
CCCAAGGACT ACTGGGGGAA GTTTGGGGAG TTCTGTCATG GGTGCTCCCT GCTGATGACA 
GGGCCTTTTA TGGTGGCTGG GGAGTTCAAG TACCACCCAG AGTGCTTTGC CTGTATGAGC 
TGCAAGGTGA TCATTGAGGA TGGGGATGCA TATGCACTGG TGCAGCATGC CACCCTCTAC 
TGTGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAGAGACT CTCCACAGAG 
TCTGTTCAGG AGCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCGGC CACCACTGAA 
GGCAGGCGGG GCTTCTCCGT GTCCGTGGAG AGTGCCTGCT CCAACTACGC CACCACTGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGTCCCAACA ATCGAAACGC CATCCACCCT 
GGGGACCGCA TCCTGGAGAT CAATGGGACC CCCGTCCGCA CACTTCGAGT GGAGGAGGTG 
GAGGATGCAA TTAGCCAGAC GAGCCAGACA CTTCAGCTGT TGATTGAACA TGACCCCGTC 
TCCCAACGCC TGGACCAGCT GCGGCTGGAG GCCCGGCTCG CTCCTCACAT GCAGAATGCC 
GGACACCCCC ACGCCCTCAG CACCCTGGAC ACCAAGGAGA ATCTGGAGGG GACACTGAGG 
AGACGTTCCC TAAGGCGCAG TAACAGTATC TCCAAGTCCC CTGGCCCCAG CTCCCCAAAG 
GAGCCCCTGC TGTTCAGCCG TGACATCAGC CGCTCAGAAT CCCTTCGTTG TTCCAGCAGC 
TATTCACAGC AGATCTTCCG GCCCTGTGAC CTAATCCATG GGGAGGTCCT GGGGAAGGGC 
TTCTTTGGGC AGGCTATCAA GGTGACACAC AAAGCCACGG GCAAAGTGAT GGTCATGAAA 
GAGTTAATTC GATGTGATGA GGAGACCCAG AAAACTTTTC TGACTGAGGT GAAAGTGATG 
CGCAGCCTGG ACCACCCCAA TGTGCTCAAG TTCATTGGTG TGCTGTACAA GGATAAGAAG 
CTGAACCTCC TGACAGAGTA CATTGAGGGG GGCACACTGA AGGACTTTCT GCGCAGTATG 
GATCCGTTCC CCTGGCAGCA GAAGGTCAGG TTTGCCAAAG GAATCGCCTC CGGAATGGCC 
TATTTGCACT CTATGTGCAT CATCCACCGG GATCTGAACT CGCACAACTG CCTCATCAAG 
TTGGACAAGA CTGTGGTGGT GGCAGACTTT GGGCTGTCAC GGCTCATAGT GGAAGAGAGG 
AAAAGGGCCC CCATGGAGAA GGCCACCACC AAGAAACGCA CCTTGCGCAA GAACGACCGC 
AAGAAGCGCT ACACGGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAACGGA 
AAGAGCTATG ATGAGACGGT GGATATCTTC TCCTTTGGGA TCGTTCTCTG TGAGATCATT 
GGGCAGGTGT ATGCAGATCC TGACTGCCTT CCCCGAACAC TGGACTTTGG CCTCAACGTG 
AAGCTTTTCT GGGAGAAGTT TGTTCCCACA GATTGTCCCC CGGCCTTCTT CCCGCTGGCC 
GCCATCTGCT GCAGACTGGA GCCTGAGAGC AGACCAGCAT TCTCGAAATT GGAGGACTCC 
TTTGAGGCCC TCTCCCTGTA CCTGGGGGAG CTGGGCATCC CGCTGCCTGC AGAGCTGGAG 
GAGTTGGACC ACACTGTGAG CATGCAGTAC GGCCTGACCC GGGACTCACC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIMK2 ( NM_005569.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005560.1 Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a, mRNA.

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CloneID	OHu25838
Clone ID Related Accession (Same CDS sequence)	NM_005569.4 , NM_005569.3
Accession Version	NM_005569.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1917bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	LIM domain kinase 2 isoform 2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX385507.2, D45906.1, BC013051.1 and AC002073.1. This sequence is a reference standard in the RefSeqGene project. On Aug 16, 2005 this sequence version replaced NM_005569.2. Transcript Variant: This variant (2a) differs in the 5' UTR and coding region as well as the 3' UTR and coding region, compared to variant 1. The resulting isoform (2a) is shorter and has distinct N- and C-termini compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.62527.1, SRR1660803.9195.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCCGCGC TGGCGGGTGA AGATGTCTGG AGGTGTCCAG GCTGTGGGGA CCACATTGCT 
CCAAGCCAGA TATGGTACAG GACTGTCAAC GAAACCTGGC ACGGCTCTTG CTTCCGGTGT 
TCAGAATGCC AGGATTCCCT CACCAACTGG TACTATGAGA AGGATGGGAA GCTCTACTGC 
CCCAAGGACT ACTGGGGGAA GTTTGGGGAG TTCTGTCATG GGTGCTCCCT GCTGATGACA 
GGGCCTTTTA TGGTGGCTGG GGAGTTCAAG TACCACCCAG AGTGCTTTGC CTGTATGAGC 
TGCAAGGTGA TCATTGAGGA TGGGGATGCA TATGCACTGG TGCAGCATGC CACCCTCTAC 
TGTGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAGAGACT CTCCACAGAG 
TCTGTTCAGG AGCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCGGC CACCACTGAA 
GGCAGGCGGG GCTTCTCCGT GTCCGTGGAG AGTGCCTGCT CCAACTACGC CACCACTGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGTCCCAACA ATCGAAACGC CATCCACCCT 
GGGGACCGCA TCCTGGAGAT CAATGGGACC CCCGTCCGCA CACTTCGAGT GGAGGAGGTG 
GAGGATGCAA TTAGCCAGAC GAGCCAGACA CTTCAGCTGT TGATTGAACA TGACCCCGTC 
TCCCAACGCC TGGACCAGCT GCGGCTGGAG GCCCGGCTCG CTCCTCACAT GCAGAATGCC 
GGACACCCCC ACGCCCTCAG CACCCTGGAC ACCAAGGAGA ATCTGGAGGG GACACTGAGG 
AGACGTTCCC TAAGGCGCAG TAACAGTATC TCCAAGTCCC CTGGCCCCAG CTCCCCAAAG 
GAGCCCCTGC TGTTCAGCCG TGACATCAGC CGCTCAGAAT CCCTTCGTTG TTCCAGCAGC 
TATTCACAGC AGATCTTCCG GCCCTGTGAC CTAATCCATG GGGAGGTCCT GGGGAAGGGC 
TTCTTTGGGC AGGCTATCAA GGTGACACAC AAAGCCACGG GCAAAGTGAT GGTCATGAAA 
GAGTTAATTC GATGTGATGA GGAGACCCAG AAAACTTTTC TGACTGAGGT GAAAGTGATG 
CGCAGCCTGG ACCACCCCAA TGTGCTCAAG TTCATTGGTG TGCTGTACAA GGATAAGAAG 
CTGAACCTCC TGACAGAGTA CATTGAGGGG GGCACACTGA AGGACTTTCT GCGCAGTATG 
GATCCGTTCC CCTGGCAGCA GAAGGTCAGG TTTGCCAAAG GAATCGCCTC CGGAATGGCC 
TATTTGCACT CTATGTGCAT CATCCACCGG GATCTGAACT CGCACAACTG CCTCATCAAG 
TTGGACAAGA CTGTGGTGGT GGCAGACTTT GGGCTGTCAC GGCTCATAGT GGAAGAGAGG 
AAAAGGGCCC CCATGGAGAA GGCCACCACC AAGAAACGCA CCTTGCGCAA GAACGACCGC 
AAGAAGCGCT ACACGGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAACGGA 
AAGAGCTATG ATGAGACGGT GGATATCTTC TCCTTTGGGA TCGTTCTCTG TGAGATCATT 
GGGCAGGTGT ATGCAGATCC TGACTGCCTT CCCCGAACAC TGGACTTTGG CCTCAACGTG 
AAGCTTTTCT GGGAGAAGTT TGTTCCCACA GATTGTCCCC CGGCCTTCTT CCCGCTGGCC 
GCCATCTGCT GCAGACTGGA GCCTGAGAGC AGACCAGCAT TCTCGAAATT GGAGGACTCC 
TTTGAGGCCC TCTCCCTGTA CCTGGGGGAG CTGGGCATCC CGCTGCCTGC AGAGCTGGAG 
GAGTTGGACC ACACTGTGAG CATGCAGTAC GGCCTGACCC GGGACTCACC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005560.1
CDS	146..2062
Translation	MSALAGEDVWRCPGCGDHIAPSQIWYRTVNETWHGSCFRCSECQDSLTNWYYEKDGKLYCPKDYWGKFGEFCHGCSLLMTGPFMVAGEFKYHPECFACMSCKVIIEDGDAYALVQHATLYCGKCHNEVVLAPMFERLSTESVQEQLPYSVTLISMPATTEGRRGFSVSVESACSNYATTVQVKEVNRMHISPNNRNAIHPGDRILEINGTPVRTLRVEEVEDAISQTSQTLQLLIEHDPVSQRLDQLRLEARLAPHMQNAGHPHALSTLDTKENLEGTLRRRSLRRSNSISKSPGPSSPKEPLLFSRDISRSESLRCSSSYSQQIFRPCDLIHGEVLGKGFFGQAIKVTHKATGKVMVMKELIRCDEETQKTFLTEVKVMRSLDHPNVLKFIGVLYKDKKLNLLTEYIEGGTLKDFLRSMDPFPWQQKVRFAKGIASGMAYLHSMCIIHRDLNSHNCLIKLDKTVVVADFGLSRLIVEERKRAPMEKATTKKRTLRKNDRKKRYTVVGNPYWMAPEMLNGKSYDETVDIFSFGIVLCEIIGQVYADPDCLPRTLDFGLNVKLFWEKFVPTDCPPAFFPLAAICCRLEPESRPAFSKLEDSFEALSLYLGELGIPLPAELEELDHTVSMQYGLTRDSPP

Target ORF information:

RefSeq Version	NM_005569.3
Organism	Homo sapiens(human)
Definition	Homo sapiens LIM domain kinase 2 (LIMK2), transcript variant 2a, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005569.3

ORF Insert Sequence:

ATGTCCGCGC TGGCGGGTGA AGATGTCTGG AGGTGTCCAG GCTGTGGGGA CCACATTGCT 
CCAAGCCAGA TATGGTACAG GACTGTCAAC GAAACCTGGC ACGGCTCTTG CTTCCGGTGT 
TCAGAATGCC AGGATTCCCT CACCAACTGG TACTATGAGA AGGATGGGAA GCTCTACTGC 
CCCAAGGACT ACTGGGGGAA GTTTGGGGAG TTCTGTCATG GGTGCTCCCT GCTGATGACA 
GGGCCTTTTA TGGTGGCTGG GGAGTTCAAG TACCACCCAG AGTGCTTTGC CTGTATGAGC 
TGCAAGGTGA TCATTGAGGA TGGGGATGCA TATGCACTGG TGCAGCATGC CACCCTCTAC 
TGTGGGAAGT GCCACAATGA GGTGGTGCTG GCACCCATGT TTGAGAGACT CTCCACAGAG 
TCTGTTCAGG AGCAGCTGCC CTACTCTGTC ACGCTCATCT CCATGCCGGC CACCACTGAA 
GGCAGGCGGG GCTTCTCCGT GTCCGTGGAG AGTGCCTGCT CCAACTACGC CACCACTGTG 
CAAGTGAAAG AGGTCAACCG GATGCACATC AGTCCCAACA ATCGAAACGC CATCCACCCT 
GGGGACCGCA TCCTGGAGAT CAATGGGACC CCCGTCCGCA CACTTCGAGT GGAGGAGGTG 
GAGGATGCAA TTAGCCAGAC GAGCCAGACA CTTCAGCTGT TGATTGAACA TGACCCCGTC 
TCCCAACGCC TGGACCAGCT GCGGCTGGAG GCCCGGCTCG CTCCTCACAT GCAGAATGCC 
GGACACCCCC ACGCCCTCAG CACCCTGGAC ACCAAGGAGA ATCTGGAGGG GACACTGAGG 
AGACGTTCCC TAAGGCGCAG TAACAGTATC TCCAAGTCCC CTGGCCCCAG CTCCCCAAAG 
GAGCCCCTGC TGTTCAGCCG TGACATCAGC CGCTCAGAAT CCCTTCGTTG TTCCAGCAGC 
TATTCACAGC AGATCTTCCG GCCCTGTGAC CTAATCCATG GGGAGGTCCT GGGGAAGGGC 
TTCTTTGGGC AGGCTATCAA GGTGACACAC AAAGCCACGG GCAAAGTGAT GGTCATGAAA 
GAGTTAATTC GATGTGATGA GGAGACCCAG AAAACTTTTC TGACTGAGGT GAAAGTGATG 
CGCAGCCTGG ACCACCCCAA TGTGCTCAAG TTCATTGGTG TGCTGTACAA GGATAAGAAG 
CTGAACCTCC TGACAGAGTA CATTGAGGGG GGCACACTGA AGGACTTTCT GCGCAGTATG 
GATCCGTTCC CCTGGCAGCA GAAGGTCAGG TTTGCCAAAG GAATCGCCTC CGGAATGGCC 
TATTTGCACT CTATGTGCAT CATCCACCGG GATCTGAACT CGCACAACTG CCTCATCAAG 
TTGGACAAGA CTGTGGTGGT GGCAGACTTT GGGCTGTCAC GGCTCATAGT GGAAGAGAGG 
AAAAGGGCCC CCATGGAGAA GGCCACCACC AAGAAACGCA CCTTGCGCAA GAACGACCGC 
AAGAAGCGCT ACACGGTGGT GGGAAACCCC TACTGGATGG CCCCTGAGAT GCTGAACGGA 
AAGAGCTATG ATGAGACGGT GGATATCTTC TCCTTTGGGA TCGTTCTCTG TGAGATCATT 
GGGCAGGTGT ATGCAGATCC TGACTGCCTT CCCCGAACAC TGGACTTTGG CCTCAACGTG 
AAGCTTTTCT GGGAGAAGTT TGTTCCCACA GATTGTCCCC CGGCCTTCTT CCCGCTGGCC 
GCCATCTGCT GCAGACTGGA GCCTGAGAGC AGACCAGCAT TCTCGAAATT GGAGGACTCC 
TTTGAGGCCC TCTCCCTGTA CCTGGGGGAG CTGGGCATCC CGCTGCCTGC AGAGCTGGAG 
GAGTTGGACC ACACTGTGAG CATGCAGTAC GGCCTGACCC GGGACTCACC TCCCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Single Nucleotide Polymorphisms of Paclitaxel-induced Peripheral Sensory Neuropathy in Chinese Han Population.
Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae39(5)593-601(2017 10)
Dou XL,Mai YL,Sun Z,Wang YY,Shao YJ,Cheng YJ,Zhou N,Luo F,Zhang B,Bai CM,Ma SQ

The role of the IL-8 signaling pathway in the infiltration of granulocytes into the livers of patients with alcoholic hepatitis.
Experimental and molecular pathology103(2)137-140(2017 10)
French SW,Mendoza AS,Afifiyan N,Tillman B,Vitocruz E,French BA

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LIMK2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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