LIM2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIM2
Entrez Gene ID	3982
Full Name	lens intrinsic membrane protein 2
Synonyms	CTRCT19,MP17,MP19
General protein information	Preferred Names lens intrinsic membrane protein 2 Names lens fiber membrane intrinsic protein MP18 MP20 lens intrinsic membrane protein 2, 19kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19q13.41
Summary	This gene encodes an eye lens-specific protein found at the junctions of lens fiber cells, where it may contribute to cell junctional organization. It acts as a receptor for calmodulin, and may play an important role in both lens development and cataractogenesis. Mutations in this gene have been associated with cataract formation. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009].
Disorder MIM:	154045
Disorder Html:	Cataract 19, multiple types, 615277 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_030657.3	NP_085915.2	lens fiber membrane intrinsic protein isoform 1
NM_001161748.2	NP_001155220.1	lens fiber membrane intrinsic protein isoform 2
NM_030657.4	NP_085915.2	lens fiber membrane intrinsic protein isoform 1
NM_001161748.1	NP_001155220.1	lens fiber membrane intrinsic protein isoform 2

Pathways from BioSystems

Homologies

	LIM2	XP_001116349.1
Mus musculus (house mouse)	Lim2	NP_808361.1
Danio rerio (zebrafish)	lim2.5	NP_001013540.1
Homo sapiens (human)	LIM2	NP_085915.2
Pan troglodytes (chimpanzee)	LIM2	XP_001174186.1
Xenopus tropicalis (tropical clawed frog)	lim2	NP_001096249.1
Rattus norvegicus (Norway rat)	Lim2	NP_446223.1
Canis lupus familiaris (dog)	LIM2	XP_003638853.1
Bos taurus (cattle)	LIM2	NP_776527.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutation in LIM2 Is Responsible for Autosomal Recessive Congenital Cataracts.
Irum B, Khan SY, Ali M, Kaul H, Kabir F, Rauf B, Fatima F, Nadeem R, Khan AO, Al Obaisi S, Naeem MA, Nasir IA, Khan SN, Husnain T, Riazuddin S, Akram J, Eghrari AO, Riazuddin SA
PloS one11(11)e0162620(2016)

Absence of mutations in four genes encoding for congenital cataract and expressed in the human brain in Tunisian families with cataract and mental retardation.
Chograni M, Chaabouni M, M?azoul F, Bouzid H, Kraiem A, Chaabouni HB
BMC ophthalmology1135(2011 Nov)

Genetic variations in GJA3, GJA8, LIM2, and age-related cataract in the Chinese population: a mutation screening study.
Zhou Z, Wang B, Hu S, Zhang C, Ma X, Qi Y
Molecular vision17621-6(2011 Feb)

A missense mutation in LIM2 causes autosomal recessive congenital cataract.
Ponnam SP, Ramesha K, Tejwani S, Matalia J, Kannabiran C
Molecular vision141204-8(2008 Jun)

Identification of a lens-specific cis-acting element within the basal promoter of the human lens intrinsic membrane protein MP19 gene (LIM2).
Hsu H, Zhang SR, Sang JL, Qi RM, Church RL
Sheng wu gong cheng xue bao = Chinese journal of biotechnology20(4)507-15(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A novel missense mutation in LIM2 is responsible for autosomal recessive congenital cataracts.
Title: Mutation in LIM2 Is Responsible for Autosomal Recessive Congenital Cataracts.

we report the absence of mutations in all studied genes in four families with phenotypes associating cataract, mental retardation and microcephaly.
Title: Absence of mutations in four genes encoding for congenital cataract and expressed in the human brain in Tunisian families with cataract and mental retardation.

The genetic mutation in GJA3, GJA8, and LIM2 may slightly contribute to the development of age-related cataracts.
Title: Genetic variations in GJA3, GJA8, LIM2, and age-related cataract in the Chinese population: a mutation screening study.

This study shows the involvement of LIM2 in human congenital cataract.
Title: A missense mutation in LIM2 causes autosomal recessive congenital cataract.

Since the LIM2 gene promoter does not contain a classic TATA box, the Hsu element may serve as the site for binding the RNA polymerase complex.
Title: Identification of a lens-specific cis-acting element within the basal promoter of the human lens intrinsic membrane protein MP19 gene (LIM2).

The following LIM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18001	NM_030657.4 Latest version!	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu03246	NM_001161748.2 Latest version!	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu18001	NM_030657.3	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu03246	NM_001161748.1	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIM2 ( NM_030657.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_085915.2 Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18001
Clone ID Related Accession (Same CDS sequence)	NM_030657.3 , NM_030657.4
Accession Version	NM_030657.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	lens fiber membrane intrinsic protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD675859.1, AF340019.1 and BU734982.1. This sequence is a reference standard in the RefSeqGene project. On Jun 13, 2009 this sequence version replaced NM_030657.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF340019.1, BC069430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163105, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGGTGAG 
CCCCCCGGCC AGGGTCCAGG CCGCGCCTGG GGAAAGAGCA GGGCGGACCT CGGGGCCCAA 
GGACACCTGT ATTCCAGATG GAGAACTCTG CGGCTCAAAG AGGGAAAGGG AGCAACCCAA 
GCATACTGGA ATGCCACCCG GGCCTTCATG ATCCTGTCTG CCCTATGCGC CATCTCCGGC 
ATCATCATGG GCATCATGGC CTTCGCTCAT CAGCCTACCT TCTCCCGCAT CTCCCGGCCC 
TTCTCTGCTG GCATCATGTT TTTTTCCTCA ACCCTTTTCG TCGTGTTGGC CTTGGCCATC 
TACACTGGAG TCACCGTCAG CTTCCTGGGC CGCCGCTTTG GGGACTGGCG CTTTTCCTGG 
TCCTACATCC TGGGCTGGGT GGCAGTGCTC ATGACGTTCT TCGCAGGGAT TTTCTACATG 
TGCGCCTACC GGGTGCATGA ATGCCGGCGC CTGTCTACAC CCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085915.2
CDS	45..692
Translation	MYSFMGGGLFCAWVGTILLVVAMATDHWMQYRLSGSFAHQGLWRYCLGNKCYLQTDSIGEPPGQGPGRAWGKSRADLGAQGHLYSRWRTLRLKEGKGATQAYWNATRAFMILSALCAISGIIMGIMAFAHQPTFSRISRPFSAGIMFFSSTLFVVLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVAVLMTFFAGIFYMCAYRVHECRRLSTPR

Target ORF information:

RefSeq Version	NM_030657.3
Organism	Homo sapiens(human)
Definition	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030657.3

ORF Insert Sequence:

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGGTGAG 
CCCCCCGGCC AGGGTCCAGG CCGCGCCTGG GGAAAGAGCA GGGCGGACCT CGGGGCCCAA 
GGACACCTGT ATTCCAGATG GAGAACTCTG CGGCTCAAAG AGGGAAAGGG AGCAACCCAA 
GCATACTGGA ATGCCACCCG GGCCTTCATG ATCCTGTCTG CCCTATGCGC CATCTCCGGC 
ATCATCATGG GCATCATGGC CTTCGCTCAT CAGCCTACCT TCTCCCGCAT CTCCCGGCCC 
TTCTCTGCTG GCATCATGTT TTTTTCCTCA ACCCTTTTCG TCGTGTTGGC CTTGGCCATC 
TACACTGGAG TCACCGTCAG CTTCCTGGGC CGCCGCTTTG GGGACTGGCG CTTTTCCTGG 
TCCTACATCC TGGGCTGGGT GGCAGTGCTC ATGACGTTCT TCGCAGGGAT TTTCTACATG 
TGCGCCTACC GGGTGCATGA ATGCCGGCGC CTGTCTACAC CCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIM2 ( NM_030657.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_085915.2 Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18001
Clone ID Related Accession (Same CDS sequence)	NM_030657.3 , NM_030657.4
Accession Version	NM_030657.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	lens fiber membrane intrinsic protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008750.9, CD675859.1, AF340019.1 and BU734982.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_030657.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF340019.1, BC069430.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163105, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGGTGAG 
CCCCCCGGCC AGGGTCCAGG CCGCGCCTGG GGAAAGAGCA GGGCGGACCT CGGGGCCCAA 
GGACACCTGT ATTCCAGATG GAGAACTCTG CGGCTCAAAG AGGGAAAGGG AGCAACCCAA 
GCATACTGGA ATGCCACCCG GGCCTTCATG ATCCTGTCTG CCCTATGCGC CATCTCCGGC 
ATCATCATGG GCATCATGGC CTTCGCTCAT CAGCCTACCT TCTCCCGCAT CTCCCGGCCC 
TTCTCTGCTG GCATCATGTT TTTTTCCTCA ACCCTTTTCG TCGTGTTGGC CTTGGCCATC 
TACACTGGAG TCACCGTCAG CTTCCTGGGC CGCCGCTTTG GGGACTGGCG CTTTTCCTGG 
TCCTACATCC TGGGCTGGGT GGCAGTGCTC ATGACGTTCT TCGCAGGGAT TTTCTACATG 
TGCGCCTACC GGGTGCATGA ATGCCGGCGC CTGTCTACAC CCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_085915.2
CDS	63..710
Translation	MYSFMGGGLFCAWVGTILLVVAMATDHWMQYRLSGSFAHQGLWRYCLGNKCYLQTDSIGEPPGQGPGRAWGKSRADLGAQGHLYSRWRTLRLKEGKGATQAYWNATRAFMILSALCAISGIIMGIMAFAHQPTFSRISRPFSAGIMFFSSTLFVVLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVAVLMTFFAGIFYMCAYRVHECRRLSTPR

Target ORF information:

RefSeq Version	NM_030657.4
Organism	Homo sapiens(human)
Definition	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_030657.4

ORF Insert Sequence:

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGGTGAG 
CCCCCCGGCC AGGGTCCAGG CCGCGCCTGG GGAAAGAGCA GGGCGGACCT CGGGGCCCAA 
GGACACCTGT ATTCCAGATG GAGAACTCTG CGGCTCAAAG AGGGAAAGGG AGCAACCCAA 
GCATACTGGA ATGCCACCCG GGCCTTCATG ATCCTGTCTG CCCTATGCGC CATCTCCGGC 
ATCATCATGG GCATCATGGC CTTCGCTCAT CAGCCTACCT TCTCCCGCAT CTCCCGGCCC 
TTCTCTGCTG GCATCATGTT TTTTTCCTCA ACCCTTTTCG TCGTGTTGGC CTTGGCCATC 
TACACTGGAG TCACCGTCAG CTTCCTGGGC CGCCGCTTTG GGGACTGGCG CTTTTCCTGG 
TCCTACATCC TGGGCTGGGT GGCAGTGCTC ATGACGTTCT TCGCAGGGAT TTTCTACATG 
TGCGCCTACC GGGTGCATGA ATGCCGGCGC CTGTCTACAC CCCGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIM2 ( NM_001161748.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001155220.1 Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03246
Clone ID Related Accession (Same CDS sequence)	NM_001161748.2 , NM_001161748.1
Accession Version	NM_001161748.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	lens fiber membrane intrinsic protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008750.9, CD675859.1, AF340020.1 and BU734982.1. On Jun 2, 2019 this sequence version replaced NM_001161748.1. Transcript Variant: This variant (2) uses an alternate, in-frame donor splice site at the 5' coding exon compared to variant 1. This results in a shorter isoform (2) missing a 42 aa protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF340020.1, CD675859.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163105, SAMN01820687 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGCATAC 
TGGAATGCCA CCCGGGCCTT CATGATCCTG TCTGCCCTAT GCGCCATCTC CGGCATCATC 
ATGGGCATCA TGGCCTTCGC TCATCAGCCT ACCTTCTCCC GCATCTCCCG GCCCTTCTCT 
GCTGGCATCA TGTTTTTTTC CTCAACCCTT TTCGTCGTGT TGGCCTTGGC CATCTACACT 
GGAGTCACCG TCAGCTTCCT GGGCCGCCGC TTTGGGGACT GGCGCTTTTC CTGGTCCTAC 
ATCCTGGGCT GGGTGGCAGT GCTCATGACG TTCTTCGCAG GGATTTTCTA CATGTGCGCC 
TACCGGGTGC ATGAATGCCG GCGCCTGTCT ACACCCCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155220.1
CDS	63..584
Translation	MYSFMGGGLFCAWVGTILLVVAMATDHWMQYRLSGSFAHQGLWRYCLGNKCYLQTDSIAYWNATRAFMILSALCAISGIIMGIMAFAHQPTFSRISRPFSAGIMFFSSTLFVVLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVAVLMTFFAGIFYMCAYRVHECRRLSTPR

Target ORF information:

RefSeq Version	NM_001161748.2
Organism	Homo sapiens(human)
Definition	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161748.2

ORF Insert Sequence:

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGCATAC 
TGGAATGCCA CCCGGGCCTT CATGATCCTG TCTGCCCTAT GCGCCATCTC CGGCATCATC 
ATGGGCATCA TGGCCTTCGC TCATCAGCCT ACCTTCTCCC GCATCTCCCG GCCCTTCTCT 
GCTGGCATCA TGTTTTTTTC CTCAACCCTT TTCGTCGTGT TGGCCTTGGC CATCTACACT 
GGAGTCACCG TCAGCTTCCT GGGCCGCCGC TTTGGGGACT GGCGCTTTTC CTGGTCCTAC 
ATCCTGGGCT GGGTGGCAGT GCTCATGACG TTCTTCGCAG GGATTTTCTA CATGTGCGCC 
TACCGGGTGC ATGAATGCCG GCGCCTGTCT ACACCCCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIM2 ( NM_001161748.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001155220.1 Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu03246
Clone ID Related Accession (Same CDS sequence)	NM_001161748.2 , NM_001161748.1
Accession Version	NM_001161748.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	lens fiber membrane intrinsic protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CD675859.1, AF340020.1 and BU734982.1. Transcript Variant: This variant (2) uses an alternate, in-frame donor splice site at the 5' coding exon compared to variant 1. This results in a shorter isoform (2) missing a 42 aa protein segment compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CD675859.1, AF340020.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2163105, SAMN01820687 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGCATAC 
TGGAATGCCA CCCGGGCCTT CATGATCCTG TCTGCCCTAT GCGCCATCTC CGGCATCATC 
ATGGGCATCA TGGCCTTCGC TCATCAGCCT ACCTTCTCCC GCATCTCCCG GCCCTTCTCT 
GCTGGCATCA TGTTTTTTTC CTCAACCCTT TTCGTCGTGT TGGCCTTGGC CATCTACACT 
GGAGTCACCG TCAGCTTCCT GGGCCGCCGC TTTGGGGACT GGCGCTTTTC CTGGTCCTAC 
ATCCTGGGCT GGGTGGCAGT GCTCATGACG TTCTTCGCAG GGATTTTCTA CATGTGCGCC 
TACCGGGTGC ATGAATGCCG GCGCCTGTCT ACACCCCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001155220.1
CDS	45..566
Translation	MYSFMGGGLFCAWVGTILLVVAMATDHWMQYRLSGSFAHQGLWRYCLGNKCYLQTDSIAYWNATRAFMILSALCAISGIIMGIMAFAHQPTFSRISRPFSAGIMFFSSTLFVVLALAIYTGVTVSFLGRRFGDWRFSWSYILGWVAVLMTFFAGIFYMCAYRVHECRRLSTPR

Target ORF information:

RefSeq Version	NM_001161748.1
Organism	Homo sapiens(human)
Definition	Homo sapiens lens intrinsic membrane protein 2 (LIM2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001161748.1

ORF Insert Sequence:

ATGTACAGCT TCATGGGTGG TGGCCTGTTC TGTGCCTGGG TGGGGACCAT CCTCCTGGTG 
GTGGCCATGG CAACAGACCA CTGGATGCAG TACCGGCTGT CAGGGTCCTT CGCCCACCAG 
GGCCTGTGGC GGTACTGCCT GGGCAACAAG TGCTACCTGC AGACAGACAG CATCGCATAC 
TGGAATGCCA CCCGGGCCTT CATGATCCTG TCTGCCCTAT GCGCCATCTC CGGCATCATC 
ATGGGCATCA TGGCCTTCGC TCATCAGCCT ACCTTCTCCC GCATCTCCCG GCCCTTCTCT 
GCTGGCATCA TGTTTTTTTC CTCAACCCTT TTCGTCGTGT TGGCCTTGGC CATCTACACT 
GGAGTCACCG TCAGCTTCCT GGGCCGCCGC TTTGGGGACT GGCGCTTTTC CTGGTCCTAC 
ATCCTGGGCT GGGTGGCAGT GCTCATGACG TTCTTCGCAG GGATTTTCTA CATGTGCGCC 
TACCGGGTGC ATGAATGCCG GCGCCTGTCT ACACCCCGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutation in LIM2 Is Responsible for Autosomal Recessive Congenital Cataracts.
PloS one11(11)e0162620(2016)
Irum B,Khan SY,Ali M,Kaul H,Kabir F,Rauf B,Fatima F,Nadeem R,Khan AO,Al Obaisi S,Naeem MA,Nasir IA,Khan SN,Husnain T,Riazuddin S,Akram J,Eghrari AO,Riazuddin SA

Absence of mutations in four genes encoding for congenital cataract and expressed in the human brain in Tunisian families with cataract and mental retardation.
BMC ophthalmology1135(2011 Nov)
Chograni M,Chaabouni M,M?azoul F,Bouzid H,Kraiem A,Chaabouni HB

Genetic variations in GJA3, GJA8, LIM2, and age-related cataract in the Chinese population: a mutation screening study.
Molecular vision17621-6(2011 Feb)
Zhou Z,Wang B,Hu S,Zhang C,Ma X,Qi Y

A missense mutation in LIM2 causes autosomal recessive congenital cataract.
Molecular vision141204-8(2008 Jun)
Ponnam SP,Ramesha K,Tejwani S,Matalia J,Kannabiran C

Identification of a lens-specific cis-acting element within the basal promoter of the human lens intrinsic membrane protein MP19 gene (LIM2).
Sheng wu gong cheng xue bao = Chinese journal of biotechnology20(4)507-15(2004 Jul)
Hsu H,Zhang SR,Sang JL,Qi RM,Church RL

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LIM2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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