LIG4 cDNA ORF clone, Homo sapiens(human)

The following LIG4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu13291 NM_206937.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_002312.3
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352598.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352599.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352600.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352602.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001098268.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352601.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352603.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 10, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_017020571.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_011521091.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_005254058.4
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_011521092.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_017020570.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_006719952.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_005254057.4
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_006719951.3
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_005254056.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_017020569.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu84536 NM_001330595.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu84536 XM_017020573.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu84534 NM_001352604.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant 11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu84534 XM_017020565.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu84534 XM_017020564.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu84534 XM_017020563.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu84534 XM_017020566.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu84534 XM_017020567.1
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu84535 XM_017020568.2
Latest version!
Homo sapiens DNA ligase 4 (LIG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
View Old Accession Versions >>
OHu13291 XM_005254057.3 Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352598.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_005254058.3 Homo sapiens DNA ligase 4 (LIG4), transcript variant X16, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352601.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_005254058.2 Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_011521092.1 Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 XM_006719951.2 Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_206937.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001098268.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352599.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu84535 XM_017020568.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu13291 XM_011521091.1 Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352602.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
OHu13291 NM_001352600.1 Homo sapiens DNA ligase 4 (LIG4), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$189.50-$265.30
$379.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352603.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-28
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. Transcript Variant: This variant (10), as well as variants 1-3 and 5-9, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM803759.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2147975, SAMN01820693 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339532.1
    CDS129..2864
    Translation

    Target ORF information:

    RefSeq Version NM_001352603.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352603.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_206937.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC037491.2 and BX648833.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 and 5-10 all encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037491.2, SRR1660803.134525.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996820.1
    CDS153..2888
    Translation

    Target ORF information:

    RefSeq Version NM_206937.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352598.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 18, 2017 this sequence version replaced XM_006719952.1. Transcript Variant: This variant (5), as well as variants 1-3 and 6-10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.41068.1, DA401268.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687, SAMN01820689 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339527.1
    CDS653..3388
    Translation

    Target ORF information:

    RefSeq Version NM_001352598.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352598.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352598.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 2, 2019 this sequence version replaced NM_001352598.1. Transcript Variant: This variant (5), as well as variants 1-3 and 6-10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.41068.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820687, SAMN01820689 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339527.1
    CDS634..3369
    Translation

    Target ORF information:

    RefSeq Version NM_001352598.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352598.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352600.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 18, 2017 this sequence version replaced XM_017020570.1. Transcript Variant: This variant (7), as well as variants 1-3, 5, 6, and 8-10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.254252.1, SRR1803614.212731.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339529.1
    CDS569..3304
    Translation

    Target ORF information:

    RefSeq Version NM_001352600.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352600.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352601.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 18, 2017 this sequence version replaced XM_005254057.4. Transcript Variant: This variant (8), as well as variants 1-3, 5-7, 9, and 10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.68316.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2147975 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339530.1
    CDS288..3023
    Translation

    Target ORF information:

    RefSeq Version NM_001352601.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352601.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352602.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 2, 2019 this sequence version replaced NM_001352602.1. Transcript Variant: This variant (9), as well as variants 1-3, 5-8, and 10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.61728.1, SRR1660803.162983.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339531.1
    CDS151..2886
    Translation

    Target ORF information:

    RefSeq Version NM_001352602.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352602.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352601.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 2, 2019 this sequence version replaced NM_001352601.1. Transcript Variant: This variant (8), as well as variants 1-3, 5-7, 9, and 10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.68316.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1968968, SAMEA2147975 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339530.1
    CDS259..2994
    Translation

    Target ORF information:

    RefSeq Version NM_001352601.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352601.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001098268.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648833.1 and BC037491.2. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 and 5-10 all encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX648833.1, SRR1660809.17745.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091738.1
    CDS236..2971
    Translation

    Target ORF information:

    RefSeq Version NM_001098268.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098268.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352599.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 18, 2017 this sequence version replaced XM_011521091.2. Transcript Variant: This variant (6), as well as variants 1-3, 5, and 7-10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.179778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339528.1
    CDS677..3412
    Translation

    Target ORF information:

    RefSeq Version NM_001352599.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352599.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_206937.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13, BC037491.2 and BX648833.1. On Jun 2, 2019 this sequence version replaced NM_206937.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1-3 and 5-10 all encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC037491.2, SRR1660805.155260.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_996820.1
    CDS155..2890
    Translation

    Target ORF information:

    RefSeq Version NM_206937.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_206937.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001098268.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX648833.1 and BC037491.2. On Jun 2, 2019 this sequence version replaced NM_001098268.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1-3 and 5-10 all encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.17745.1, SRR1803614.209931.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091738.1
    CDS217..2952
    Translation

    Target ORF information:

    RefSeq Version NM_001098268.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098268.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352599.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 2, 2019 this sequence version replaced NM_001352599.1. Transcript Variant: This variant (6), as well as variants 1-3, 5, and 7-10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.179778.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339528.1
    CDS658..3393
    Translation

    Target ORF information:

    RefSeq Version NM_001352599.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352599.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352602.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 18, 2017 this sequence version replaced XM_011521092.2. Transcript Variant: This variant (9), as well as variants 1-3, 5-8, and 10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.179148.1, SRR1660803.162983.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339531.1
    CDS180..2915
    Translation

    Target ORF information:

    RefSeq Version NM_001352602.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352602.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_002312.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X83441.1, BC037491.2, CD102896.1 and AI809944.1. This sequence is a reference standard in the RefSeqGene project. On Apr 7, 2004 this sequence version replaced NM_002312.2. Transcript Variant: This variant (1), as well as variants 2, 3, and 5-10, encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X83441.1, SRR1660807.214250.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2147975, SAMN01820693 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_002303.2
    CDS274..3009
    Translation

    Target ORF information:

    RefSeq Version NM_002312.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_002312.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version NM_001352600.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 2, 2019 this sequence version replaced NM_001352600.1. Transcript Variant: This variant (7), as well as variants 1-3, 5, 6, and 8-10, encodes isoform 1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660807.254252.1, SRR1803617.271917.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820693 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339529.1
    CDS550..3285
    Translation

    Target ORF information:

    RefSeq Version NM_001352600.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352600.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_017020569.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876058.1
    CDS2256..4991
    Translation

    Target ORF information:

    RefSeq Version XM_017020569.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_005254057.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254057.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254114.1
    CDS288..3023
    Translation

    Target ORF information:

    RefSeq Version XM_005254057.4
    Organism Homo sapiens(Human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254057.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_005254057.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254114.1
    Translation

    Target ORF information:

    RefSeq Version XM_005254057.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_005254058.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254058.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254115.1
    CDS1734..4469
    Translation

    Target ORF information:

    RefSeq Version XM_005254058.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X16, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254058.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_005254056.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254113.1
    CDS118..2853
    Translation

    Target ORF information:

    RefSeq Version XM_005254056.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254056.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_011521091.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521091.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519393.1
    CDS615..3350
    Translation

    Target ORF information:

    RefSeq Version XM_011521091.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521091.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_011521092.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519394.1
    Translation

    Target ORF information:

    RefSeq Version XM_011521092.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521092.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_017020571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876060.1
    CDS2340..5075
    Translation

    Target ORF information:

    RefSeq Version XM_017020571.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_005254058.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005254058.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254115.1
    CDS1962..4697
    Translation

    Target ORF information:

    RefSeq Version XM_005254058.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254058.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_005254058.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254115.1
    Translation

    Target ORF information:

    RefSeq Version XM_005254058.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254058.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_006719951.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720014.1
    Translation

    Target ORF information:

    RefSeq Version XM_006719951.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006719951.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_011521092.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521092.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519394.1
    CDS191..2926
    Translation

    Target ORF information:

    RefSeq Version XM_011521092.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521092.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_017020570.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876059.1
    CDS535..3270
    Translation

    Target ORF information:

    RefSeq Version XM_017020570.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_006719952.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720015.1
    CDS586..3321
    Translation

    Target ORF information:

    RefSeq Version XM_006719952.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006719952.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_006719951.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006719951.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006720014.1
    CDS1993..4728
    Translation

    Target ORF information:

    RefSeq Version XM_006719951.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006719951.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu13291
    Clone ID Related Accession (Same CDS sequence) XM_017020569.1 , XM_005254057.4 , XM_005254057.3 , XM_005254058.3 , NM_001352603.1 , XM_005254056.1 , XM_011521091.2 , XM_011521092.1 , NM_206937.1 , NM_001352598.1 , NM_001352598.2 , NM_001352600.1 , XM_017020571.1 , XM_005254058.4 , NM_001352601.1 , NM_001352602.2 , NM_001352601.2 , XM_005254058.2 , XM_006719951.2 , XM_011521092.2 , XM_017020570.1 , XM_006719952.1 , XM_006719951.3 , NM_001098268.1 , NM_001352599.1 , NM_206937.2 , NM_001098268.2 , NM_001352599.2 , XM_011521091.1 , NM_001352602.1 , NM_002312.3 , NM_001352600.2
    Accession Version XM_011521091.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011519393.1
    Translation

    Target ORF information:

    RefSeq Version XM_011521091.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens ligase IV, DNA, ATP-dependent (LIG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521091.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCTGCCT CACAAACTTC ACAAACTGTT GCATCTCACG TTCCTTTTGC AGATTTGTGT 
    TCAACTTTAG AACGAATACA GAAAAGTAAA GGACGTGCAG AAAAAATCAG ACACTTCAGG
    GAATTTTTAG ATTCTTGGAG AAAATTTCAT GATGCTCTTC ATAAGAACCA CAAAGATGTC
    ACAGACTCTT TTTATCCAGC AATGAGACTA ATTCTTCCTC AGCTAGAAAG AGAGAGAATG
    GCCTATGGAA TTAAAGAAAC TATGCTTGCT AAGCTTTATA TTGAGTTGCT TAATTTACCT
    AGAGATGGAA AAGATGCCCT CAAACTTTTA AACTACAGAA CACCCACTGG AACTCATGGA
    GATGCTGGAG ACTTTGCAAT GATTGCATAT TTTGTGTTGA AGCCAAGATG TTTACAGAAA
    GGAAGTTTAA CCATACAGCA AGTAAACGAC CTTTTAGACT CAATTGCCAG CAATAATTCT
    GCTAAAAGAA AAGACCTAAT AAAAAAGAGC CTTCTTCAAC TTATAACTCA GAGTTCAGCA
    CTTGAGCAAA AGTGGCTTAT ACGGATGATC ATAAAGGATT TAAAGCTTGG TGTTAGTCAG
    CAAACTATCT TTTCTGTTTT TCATAATGAT GCTGCTGAGT TGCATAATGT CACTACAGAT
    CTGGAAAAAG TCTGTAGGCA ACTGCATGAT CCTTCTGTAG GACTCAGTGA TATTTCTATC
    ACTTTATTTT CTGCATTTAA ACCAATGCTA GCTGCTATTG CAGATATTGA GCACATTGAG
    AAGGATATGA AACATCAGAG TTTCTACATA GAAACCAAGC TAGATGGTGA ACGTATGCAA
    ATGCACAAAG ATGGAGATGT ATATAAATAC TTCTCTCGAA ATGGATATAA CTACACTGAT
    CAGTTTGGTG CTTCTCCTAC TGAAGGTTCT CTTACCCCAT TCATTCATAA TGCATTCAAA
    GCAGATATAC AAATCTGTAT TCTTGATGGT GAGATGATGG CCTATAATCC TAATACACAA
    ACTTTCATGC AAAAGGGAAC TAAGTTTGAT ATTAAAAGAA TGGTAGAGGA TTCTGATCTG
    CAAACTTGTT ATTGTGTTTT TGATGTATTG ATGGTTAATA ATAAAAAGCT AGGGCATGAG
    ACTCTGAGAA AGAGGTATGA GATTCTTAGT AGTATTTTTA CACCAATTCC AGGTAGAATA
    GAAATAGTGC AGAAAACACA AGCTCATACT AAGAATGAAG TAATTGATGC ATTGAATGAA
    GCAATAGATA AAAGAGAAGA GGGAATTATG GTAAAACAAC CTCTATCCAT CTACAAGCCA
    GACAAAAGAG GTGAAGGGTG GTTAAAAATT AAACCAGAGT ATGTCAGTGG ACTAATGGAT
    GAATTGGACA TTTTAATTGT TGGAGGATAT TGGGGTAAAG GATCACGGGG TGGAATGATG
    TCTCATTTTC TGTGTGCAGT AGCAGAGAAG CCCCCTCCTG GTGAGAAGCC ATCTGTGTTT
    CATACTCTCT CTCGTGTTGG GTCTGGCTGC ACCATGAAAG AACTGTATGA TCTGGGTTTG
    AAATTGGCCA AGTATTGGAA GCCTTTTCAT AGAAAAGCTC CACCAAGCAG CATTTTATGT
    GGAACAGAGA AGCCAGAAGT ATACATTGAA CCTTGTAATT CTGTCATTGT TCAGATTAAA
    GCAGCAGAGA TCGTACCCAG TGATATGTAT AAAACTGGCT GCACCTTGCG TTTTCCACGA
    ATTGAAAAGA TAAGAGATGA CAAGGAGTGG CATGAGTGCA TGACCCTGGA CGACCTAGAA
    CAACTTAGGG GGAAGGCATC TGGTAAGCTC GCATCTAAAC ACCTTTATAT AGGTGGTGAT
    GATGAACCAC AAGAAAAAAA GCGGAAAGCT GCCCCAAAGA TGAAGAAAGT TATTGGAATT
    ATTGAGCACT TAAAAGCACC TAACCTTACT AACGTTAACA AAATTTCTAA TATATTTGAA
    GATGTAGAGT TTTGTGTTAT GAGTGGAACA GATAGCCAGC CAAAGCCTGA CCTGGAGAAC
    AGAATTGCAG AATTTGGTGG TTATATAGTA CAAAATCCAG GCCCAGACAC GTACTGTGTA
    ATTGCAGGGT CTGAGAACAT CAGAGTGAAA AACATAATTT TGTCAAATAA ACATGATGTT
    GTCAAGCCTG CATGGCTTTT AGAATGTTTT AAGACCAAAA GCTTTGTACC ATGGCAGCCT
    CGCTTTATGA TTCATATGTG CCCATCAACC AAAGAACATT TTGCCCGTGA ATATGATTGC
    TATGGTGATA GTTATTTCAT TGATACAGAC TTGAACCAAC TGAAGGAAGT ATTCTCAGGA
    ATTAAAAATT CTAACGAGCA GACTCCTGAA GAAATGGCTT CTCTGATTGC TGATTTAGAA
    TATCGGTATT CCTGGGATTG CTCTCCTCTC AGTATGTTTC GACGCCACAC CGTTTATTTG
    GACTCGTATG CTGTTATTAA TGACCTGAGT ACCAAAAATG AGGGGACAAG GTTAGCTATT
    AAAGCCTTGG AGCTTCGGTT TCATGGAGCA AAAGTAGTTT CTTGTTTAGC TGAGGGAGTG
    TCTCATGTAA TAATTGGGGA AGATCATAGT CGTGTTGCAG ATTTTAAAGC TTTTAGAAGA
    ACTTTTAAGA GAAAGTTTAA AATCCTAAAA GAAAGTTGGG TAACTGATTC AATAGACAAG
    TGTGAATTAC AAGAAGAAAA CCAGTATTTG ATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84536
    Clone ID Related Accession (Same CDS sequence) XM_017020573.1 , NM_001330595.1
    Accession Version NM_001330595.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC037491.2, DC372305.1, AK292419.1 and BX648833.1. On Aug 27, 2016 this sequence version replaced XM_017020572.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a dowstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292419.1, SRR1803616.89779.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTAA TTCTTCCTCA GCTAGAAAGA GAGAGAATGG CCTATGGAAT TAAAGAAACT 
    ATGCTTGCTA AGCTTTATAT TGAGTTGCTT AATTTACCTA GAGATGGAAA AGATGCCCTC
    AAACTTTTAA ACTACAGAAC ACCCACTGGA ACTCATGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCATATT TTGTGTTGAA GCCAAGATGT TTACAGAAAG GAAGTTTAAC CATACAGCAA
    GTAAACGACC TTTTAGACTC AATTGCCAGC AATAATTCTG CTAAAAGAAA AGACCTAATA
    AAAAAGAGCC TTCTTCAACT TATAACTCAG AGTTCAGCAC TTGAGCAAAA GTGGCTTATA
    CGGATGATCA TAAAGGATTT AAAGCTTGGT GTTAGTCAGC AAACTATCTT TTCTGTTTTT
    CATAATGATG CTGCTGAGTT GCATAATGTC ACTACAGATC TGGAAAAAGT CTGTAGGCAA
    CTGCATGATC CTTCTGTAGG ACTCAGTGAT ATTTCTATCA CTTTATTTTC TGCATTTAAA
    CCAATGCTAG CTGCTATTGC AGATATTGAG CACATTGAGA AGGATATGAA ACATCAGAGT
    TTCTACATAG AAACCAAGCT AGATGGTGAA CGTATGCAAA TGCACAAAGA TGGAGATGTA
    TATAAATACT TCTCTCGAAA TGGATATAAC TACACTGATC AGTTTGGTGC TTCTCCTACT
    GAAGGTTCTC TTACCCCATT CATTCATAAT GCATTCAAAG CAGATATACA AATCTGTATT
    CTTGATGGTG AGATGATGGC CTATAATCCT AATACACAAA CTTTCATGCA AAAGGGAACT
    AAGTTTGATA TTAAAAGAAT GGTAGAGGAT TCTGATCTGC AAACTTGTTA TTGTGTTTTT
    GATGTATTGA TGGTTAATAA TAAAAAGCTA GGGCATGAGA CTCTGAGAAA GAGGTATGAG
    ATTCTTAGTA GTATTTTTAC ACCAATTCCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAATGAAGT AATTGATGCA TTGAATGAAG CAATAGATAA AAGAGAAGAG
    GGAATTATGG TAAAACAACC TCTATCCATC TACAAGCCAG ACAAAAGAGG TGAAGGGTGG
    TTAAAAATTA AACCAGAGTA TGTCAGTGGA CTAATGGATG AATTGGACAT TTTAATTGTT
    GGAGGATATT GGGGTAAAGG ATCACGGGGT GGAATGATGT CTCATTTTCT GTGTGCAGTA
    GCAGAGAAGC CCCCTCCTGG TGAGAAGCCA TCTGTGTTTC ATACTCTCTC TCGTGTTGGG
    TCTGGCTGCA CCATGAAAGA ACTGTATGAT CTGGGTTTGA AATTGGCCAA GTATTGGAAG
    CCTTTTCATA GAAAAGCTCC ACCAAGCAGC ATTTTATGTG GAACAGAGAA GCCAGAAGTA
    TACATTGAAC CTTGTAATTC TGTCATTGTT CAGATTAAAG CAGCAGAGAT CGTACCCAGT
    GATATGTATA AAACTGGCTG CACCTTGCGT TTTCCACGAA TTGAAAAGAT AAGAGATGAC
    AAGGAGTGGC ATGAGTGCAT GACCCTGGAC GACCTAGAAC AACTTAGGGG GAAGGCATCT
    GGTAAGCTCG CATCTAAACA CCTTTATATA GGTGGTGATG ATGAACCACA AGAAAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTT ATTGGAATTA TTGAGCACTT AAAAGCACCT
    AACCTTACTA ACGTTAACAA AATTTCTAAT ATATTTGAAG ATGTAGAGTT TTGTGTTATG
    AGTGGAACAG ATAGCCAGCC AAAGCCTGAC CTGGAGAACA GAATTGCAGA ATTTGGTGGT
    TATATAGTAC AAAATCCAGG CCCAGACACG TACTGTGTAA TTGCAGGGTC TGAGAACATC
    AGAGTGAAAA ACATAATTTT GTCAAATAAA CATGATGTTG TCAAGCCTGC ATGGCTTTTA
    GAATGTTTTA AGACCAAAAG CTTTGTACCA TGGCAGCCTC GCTTTATGAT TCATATGTGC
    CCATCAACCA AAGAACATTT TGCCCGTGAA TATGATTGCT ATGGTGATAG TTATTTCATT
    GATACAGACT TGAACCAACT GAAGGAAGTA TTCTCAGGAA TTAAAAATTC TAACGAGCAG
    ACTCCTGAAG AAATGGCTTC TCTGATTGCT GATTTAGAAT ATCGGTATTC CTGGGATTGC
    TCTCCTCTCA GTATGTTTCG ACGCCACACC GTTTATTTGG ACTCGTATGC TGTTATTAAT
    GACCTGAGTA CCAAAAATGA GGGGACAAGG TTAGCTATTA AAGCCTTGGA GCTTCGGTTT
    CATGGAGCAA AAGTAGTTTC TTGTTTAGCT GAGGGAGTGT CTCATGTAAT AATTGGGGAA
    GATCATAGTC GTGTTGCAGA TTTTAAAGCT TTTAGAAGAA CTTTTAAGAG AAAGTTTAAA
    ATCCTAAAAG AAAGTTGGGT AACTGATTCA ATAGACAAGT GTGAATTACA AGAAGAAAAC
    CAGTATTTGA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317524.1
    CDS274..2808
    Translation

    Target ORF information:

    RefSeq Version NM_001330595.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330595.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTAA TTCTTCCTCA GCTAGAAAGA GAGAGAATGG CCTATGGAAT TAAAGAAACT 
    ATGCTTGCTA AGCTTTATAT TGAGTTGCTT AATTTACCTA GAGATGGAAA AGATGCCCTC
    AAACTTTTAA ACTACAGAAC ACCCACTGGA ACTCATGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCATATT TTGTGTTGAA GCCAAGATGT TTACAGAAAG GAAGTTTAAC CATACAGCAA
    GTAAACGACC TTTTAGACTC AATTGCCAGC AATAATTCTG CTAAAAGAAA AGACCTAATA
    AAAAAGAGCC TTCTTCAACT TATAACTCAG AGTTCAGCAC TTGAGCAAAA GTGGCTTATA
    CGGATGATCA TAAAGGATTT AAAGCTTGGT GTTAGTCAGC AAACTATCTT TTCTGTTTTT
    CATAATGATG CTGCTGAGTT GCATAATGTC ACTACAGATC TGGAAAAAGT CTGTAGGCAA
    CTGCATGATC CTTCTGTAGG ACTCAGTGAT ATTTCTATCA CTTTATTTTC TGCATTTAAA
    CCAATGCTAG CTGCTATTGC AGATATTGAG CACATTGAGA AGGATATGAA ACATCAGAGT
    TTCTACATAG AAACCAAGCT AGATGGTGAA CGTATGCAAA TGCACAAAGA TGGAGATGTA
    TATAAATACT TCTCTCGAAA TGGATATAAC TACACTGATC AGTTTGGTGC TTCTCCTACT
    GAAGGTTCTC TTACCCCATT CATTCATAAT GCATTCAAAG CAGATATACA AATCTGTATT
    CTTGATGGTG AGATGATGGC CTATAATCCT AATACACAAA CTTTCATGCA AAAGGGAACT
    AAGTTTGATA TTAAAAGAAT GGTAGAGGAT TCTGATCTGC AAACTTGTTA TTGTGTTTTT
    GATGTATTGA TGGTTAATAA TAAAAAGCTA GGGCATGAGA CTCTGAGAAA GAGGTATGAG
    ATTCTTAGTA GTATTTTTAC ACCAATTCCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAATGAAGT AATTGATGCA TTGAATGAAG CAATAGATAA AAGAGAAGAG
    GGAATTATGG TAAAACAACC TCTATCCATC TACAAGCCAG ACAAAAGAGG TGAAGGGTGG
    TTAAAAATTA AACCAGAGTA TGTCAGTGGA CTAATGGATG AATTGGACAT TTTAATTGTT
    GGAGGATATT GGGGTAAAGG ATCACGGGGT GGAATGATGT CTCATTTTCT GTGTGCAGTA
    GCAGAGAAGC CCCCTCCTGG TGAGAAGCCA TCTGTGTTTC ATACTCTCTC TCGTGTTGGG
    TCTGGCTGCA CCATGAAAGA ACTGTATGAT CTGGGTTTGA AATTGGCCAA GTATTGGAAG
    CCTTTTCATA GAAAAGCTCC ACCAAGCAGC ATTTTATGTG GAACAGAGAA GCCAGAAGTA
    TACATTGAAC CTTGTAATTC TGTCATTGTT CAGATTAAAG CAGCAGAGAT CGTACCCAGT
    GATATGTATA AAACTGGCTG CACCTTGCGT TTTCCACGAA TTGAAAAGAT AAGAGATGAC
    AAGGAGTGGC ATGAGTGCAT GACCCTGGAC GACCTAGAAC AACTTAGGGG GAAGGCATCT
    GGTAAGCTCG CATCTAAACA CCTTTATATA GGTGGTGATG ATGAACCACA AGAAAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTT ATTGGAATTA TTGAGCACTT AAAAGCACCT
    AACCTTACTA ACGTTAACAA AATTTCTAAT ATATTTGAAG ATGTAGAGTT TTGTGTTATG
    AGTGGAACAG ATAGCCAGCC AAAGCCTGAC CTGGAGAACA GAATTGCAGA ATTTGGTGGT
    TATATAGTAC AAAATCCAGG CCCAGACACG TACTGTGTAA TTGCAGGGTC TGAGAACATC
    AGAGTGAAAA ACATAATTTT GTCAAATAAA CATGATGTTG TCAAGCCTGC ATGGCTTTTA
    GAATGTTTTA AGACCAAAAG CTTTGTACCA TGGCAGCCTC GCTTTATGAT TCATATGTGC
    CCATCAACCA AAGAACATTT TGCCCGTGAA TATGATTGCT ATGGTGATAG TTATTTCATT
    GATACAGACT TGAACCAACT GAAGGAAGTA TTCTCAGGAA TTAAAAATTC TAACGAGCAG
    ACTCCTGAAG AAATGGCTTC TCTGATTGCT GATTTAGAAT ATCGGTATTC CTGGGATTGC
    TCTCCTCTCA GTATGTTTCG ACGCCACACC GTTTATTTGG ACTCGTATGC TGTTATTAAT
    GACCTGAGTA CCAAAAATGA GGGGACAAGG TTAGCTATTA AAGCCTTGGA GCTTCGGTTT
    CATGGAGCAA AAGTAGTTTC TTGTTTAGCT GAGGGAGTGT CTCATGTAAT AATTGGGGAA
    GATCATAGTC GTGTTGCAGA TTTTAAAGCT TTTAGAAGAA CTTTTAAGAG AAAGTTTAAA
    ATCCTAAAAG AAAGTTGGGT AACTGATTCA ATAGACAAGT GTGAATTACA AGAAGAAAAC
    CAGTATTTGA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84536
    Clone ID Related Accession (Same CDS sequence) XM_017020573.1 , NM_001330595.1
    Accession Version XM_017020573.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2535bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTAA TTCTTCCTCA GCTAGAAAGA GAGAGAATGG CCTATGGAAT TAAAGAAACT 
    ATGCTTGCTA AGCTTTATAT TGAGTTGCTT AATTTACCTA GAGATGGAAA AGATGCCCTC
    AAACTTTTAA ACTACAGAAC ACCCACTGGA ACTCATGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCATATT TTGTGTTGAA GCCAAGATGT TTACAGAAAG GAAGTTTAAC CATACAGCAA
    GTAAACGACC TTTTAGACTC AATTGCCAGC AATAATTCTG CTAAAAGAAA AGACCTAATA
    AAAAAGAGCC TTCTTCAACT TATAACTCAG AGTTCAGCAC TTGAGCAAAA GTGGCTTATA
    CGGATGATCA TAAAGGATTT AAAGCTTGGT GTTAGTCAGC AAACTATCTT TTCTGTTTTT
    CATAATGATG CTGCTGAGTT GCATAATGTC ACTACAGATC TGGAAAAAGT CTGTAGGCAA
    CTGCATGATC CTTCTGTAGG ACTCAGTGAT ATTTCTATCA CTTTATTTTC TGCATTTAAA
    CCAATGCTAG CTGCTATTGC AGATATTGAG CACATTGAGA AGGATATGAA ACATCAGAGT
    TTCTACATAG AAACCAAGCT AGATGGTGAA CGTATGCAAA TGCACAAAGA TGGAGATGTA
    TATAAATACT TCTCTCGAAA TGGATATAAC TACACTGATC AGTTTGGTGC TTCTCCTACT
    GAAGGTTCTC TTACCCCATT CATTCATAAT GCATTCAAAG CAGATATACA AATCTGTATT
    CTTGATGGTG AGATGATGGC CTATAATCCT AATACACAAA CTTTCATGCA AAAGGGAACT
    AAGTTTGATA TTAAAAGAAT GGTAGAGGAT TCTGATCTGC AAACTTGTTA TTGTGTTTTT
    GATGTATTGA TGGTTAATAA TAAAAAGCTA GGGCATGAGA CTCTGAGAAA GAGGTATGAG
    ATTCTTAGTA GTATTTTTAC ACCAATTCCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAATGAAGT AATTGATGCA TTGAATGAAG CAATAGATAA AAGAGAAGAG
    GGAATTATGG TAAAACAACC TCTATCCATC TACAAGCCAG ACAAAAGAGG TGAAGGGTGG
    TTAAAAATTA AACCAGAGTA TGTCAGTGGA CTAATGGATG AATTGGACAT TTTAATTGTT
    GGAGGATATT GGGGTAAAGG ATCACGGGGT GGAATGATGT CTCATTTTCT GTGTGCAGTA
    GCAGAGAAGC CCCCTCCTGG TGAGAAGCCA TCTGTGTTTC ATACTCTCTC TCGTGTTGGG
    TCTGGCTGCA CCATGAAAGA ACTGTATGAT CTGGGTTTGA AATTGGCCAA GTATTGGAAG
    CCTTTTCATA GAAAAGCTCC ACCAAGCAGC ATTTTATGTG GAACAGAGAA GCCAGAAGTA
    TACATTGAAC CTTGTAATTC TGTCATTGTT CAGATTAAAG CAGCAGAGAT CGTACCCAGT
    GATATGTATA AAACTGGCTG CACCTTGCGT TTTCCACGAA TTGAAAAGAT AAGAGATGAC
    AAGGAGTGGC ATGAGTGCAT GACCCTGGAC GACCTAGAAC AACTTAGGGG GAAGGCATCT
    GGTAAGCTCG CATCTAAACA CCTTTATATA GGTGGTGATG ATGAACCACA AGAAAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTT ATTGGAATTA TTGAGCACTT AAAAGCACCT
    AACCTTACTA ACGTTAACAA AATTTCTAAT ATATTTGAAG ATGTAGAGTT TTGTGTTATG
    AGTGGAACAG ATAGCCAGCC AAAGCCTGAC CTGGAGAACA GAATTGCAGA ATTTGGTGGT
    TATATAGTAC AAAATCCAGG CCCAGACACG TACTGTGTAA TTGCAGGGTC TGAGAACATC
    AGAGTGAAAA ACATAATTTT GTCAAATAAA CATGATGTTG TCAAGCCTGC ATGGCTTTTA
    GAATGTTTTA AGACCAAAAG CTTTGTACCA TGGCAGCCTC GCTTTATGAT TCATATGTGC
    CCATCAACCA AAGAACATTT TGCCCGTGAA TATGATTGCT ATGGTGATAG TTATTTCATT
    GATACAGACT TGAACCAACT GAAGGAAGTA TTCTCAGGAA TTAAAAATTC TAACGAGCAG
    ACTCCTGAAG AAATGGCTTC TCTGATTGCT GATTTAGAAT ATCGGTATTC CTGGGATTGC
    TCTCCTCTCA GTATGTTTCG ACGCCACACC GTTTATTTGG ACTCGTATGC TGTTATTAAT
    GACCTGAGTA CCAAAAATGA GGGGACAAGG TTAGCTATTA AAGCCTTGGA GCTTCGGTTT
    CATGGAGCAA AAGTAGTTTC TTGTTTAGCT GAGGGAGTGT CTCATGTAAT AATTGGGGAA
    GATCATAGTC GTGTTGCAGA TTTTAAAGCT TTTAGAAGAA CTTTTAAGAG AAAGTTTAAA
    ATCCTAAAAG AAAGTTGGGT AACTGATTCA ATAGACAAGT GTGAATTACA AGAAGAAAAC
    CAGTATTTGA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876062.1
    CDS238..2772
    Translation

    Target ORF information:

    RefSeq Version XM_017020573.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020573.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    ATGAGACTAA TTCTTCCTCA GCTAGAAAGA GAGAGAATGG CCTATGGAAT TAAAGAAACT 
    ATGCTTGCTA AGCTTTATAT TGAGTTGCTT AATTTACCTA GAGATGGAAA AGATGCCCTC
    AAACTTTTAA ACTACAGAAC ACCCACTGGA ACTCATGGAG ATGCTGGAGA CTTTGCAATG
    ATTGCATATT TTGTGTTGAA GCCAAGATGT TTACAGAAAG GAAGTTTAAC CATACAGCAA
    GTAAACGACC TTTTAGACTC AATTGCCAGC AATAATTCTG CTAAAAGAAA AGACCTAATA
    AAAAAGAGCC TTCTTCAACT TATAACTCAG AGTTCAGCAC TTGAGCAAAA GTGGCTTATA
    CGGATGATCA TAAAGGATTT AAAGCTTGGT GTTAGTCAGC AAACTATCTT TTCTGTTTTT
    CATAATGATG CTGCTGAGTT GCATAATGTC ACTACAGATC TGGAAAAAGT CTGTAGGCAA
    CTGCATGATC CTTCTGTAGG ACTCAGTGAT ATTTCTATCA CTTTATTTTC TGCATTTAAA
    CCAATGCTAG CTGCTATTGC AGATATTGAG CACATTGAGA AGGATATGAA ACATCAGAGT
    TTCTACATAG AAACCAAGCT AGATGGTGAA CGTATGCAAA TGCACAAAGA TGGAGATGTA
    TATAAATACT TCTCTCGAAA TGGATATAAC TACACTGATC AGTTTGGTGC TTCTCCTACT
    GAAGGTTCTC TTACCCCATT CATTCATAAT GCATTCAAAG CAGATATACA AATCTGTATT
    CTTGATGGTG AGATGATGGC CTATAATCCT AATACACAAA CTTTCATGCA AAAGGGAACT
    AAGTTTGATA TTAAAAGAAT GGTAGAGGAT TCTGATCTGC AAACTTGTTA TTGTGTTTTT
    GATGTATTGA TGGTTAATAA TAAAAAGCTA GGGCATGAGA CTCTGAGAAA GAGGTATGAG
    ATTCTTAGTA GTATTTTTAC ACCAATTCCA GGTAGAATAG AAATAGTGCA GAAAACACAA
    GCTCATACTA AGAATGAAGT AATTGATGCA TTGAATGAAG CAATAGATAA AAGAGAAGAG
    GGAATTATGG TAAAACAACC TCTATCCATC TACAAGCCAG ACAAAAGAGG TGAAGGGTGG
    TTAAAAATTA AACCAGAGTA TGTCAGTGGA CTAATGGATG AATTGGACAT TTTAATTGTT
    GGAGGATATT GGGGTAAAGG ATCACGGGGT GGAATGATGT CTCATTTTCT GTGTGCAGTA
    GCAGAGAAGC CCCCTCCTGG TGAGAAGCCA TCTGTGTTTC ATACTCTCTC TCGTGTTGGG
    TCTGGCTGCA CCATGAAAGA ACTGTATGAT CTGGGTTTGA AATTGGCCAA GTATTGGAAG
    CCTTTTCATA GAAAAGCTCC ACCAAGCAGC ATTTTATGTG GAACAGAGAA GCCAGAAGTA
    TACATTGAAC CTTGTAATTC TGTCATTGTT CAGATTAAAG CAGCAGAGAT CGTACCCAGT
    GATATGTATA AAACTGGCTG CACCTTGCGT TTTCCACGAA TTGAAAAGAT AAGAGATGAC
    AAGGAGTGGC ATGAGTGCAT GACCCTGGAC GACCTAGAAC AACTTAGGGG GAAGGCATCT
    GGTAAGCTCG CATCTAAACA CCTTTATATA GGTGGTGATG ATGAACCACA AGAAAAAAAG
    CGGAAAGCTG CCCCAAAGAT GAAGAAAGTT ATTGGAATTA TTGAGCACTT AAAAGCACCT
    AACCTTACTA ACGTTAACAA AATTTCTAAT ATATTTGAAG ATGTAGAGTT TTGTGTTATG
    AGTGGAACAG ATAGCCAGCC AAAGCCTGAC CTGGAGAACA GAATTGCAGA ATTTGGTGGT
    TATATAGTAC AAAATCCAGG CCCAGACACG TACTGTGTAA TTGCAGGGTC TGAGAACATC
    AGAGTGAAAA ACATAATTTT GTCAAATAAA CATGATGTTG TCAAGCCTGC ATGGCTTTTA
    GAATGTTTTA AGACCAAAAG CTTTGTACCA TGGCAGCCTC GCTTTATGAT TCATATGTGC
    CCATCAACCA AAGAACATTT TGCCCGTGAA TATGATTGCT ATGGTGATAG TTATTTCATT
    GATACAGACT TGAACCAACT GAAGGAAGTA TTCTCAGGAA TTAAAAATTC TAACGAGCAG
    ACTCCTGAAG AAATGGCTTC TCTGATTGCT GATTTAGAAT ATCGGTATTC CTGGGATTGC
    TCTCCTCTCA GTATGTTTCG ACGCCACACC GTTTATTTGG ACTCGTATGC TGTTATTAAT
    GACCTGAGTA CCAAAAATGA GGGGACAAGG TTAGCTATTA AAGCCTTGGA GCTTCGGTTT
    CATGGAGCAA AAGTAGTTTC TTGTTTAGCT GAGGGAGTGT CTCATGTAAT AATTGGGGAA
    GATCATAGTC GTGTTGCAGA TTTTAAAGCT TTTAGAAGAA CTTTTAAGAG AAAGTTTAAA
    ATCCTAAAAG AAAGTTGGGT AACTGATTCA ATAGACAAGT GTGAATTACA AGAAGAAAAC
    CAGTATTTGA TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84534
    Clone ID Related Accession (Same CDS sequence) NM_001352604.1 , XM_017020567.1 , XM_017020563.1 , XM_017020564.1 , XM_017020565.1 , XM_017020566.1
    Accession Version NM_001352604.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-03
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL157762.13. On Jun 18, 2017 this sequence version replaced XM_017020567.1. Transcript Variant: This variant (11) encodes the longest isoform (3). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.171331.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001339533.1
    CDS172..2943
    Translation

    Target ORF information:

    RefSeq Version NM_001352604.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant 11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001352604.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84534
    Clone ID Related Accession (Same CDS sequence) NM_001352604.1 , XM_017020567.1 , XM_017020563.1 , XM_017020564.1 , XM_017020565.1 , XM_017020566.1
    Accession Version XM_017020567.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product DNA ligase 4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876056.1
    CDS153..2924
    Translation

    Target ORF information:

    RefSeq Version XM_017020567.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020567.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84534
    Clone ID Related Accession (Same CDS sequence) NM_001352604.1 , XM_017020567.1 , XM_017020563.1 , XM_017020564.1 , XM_017020565.1 , XM_017020566.1
    Accession Version XM_017020563.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876052.1
    CDS1799..4570
    Translation

    Target ORF information:

    RefSeq Version XM_017020563.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020563.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84534
    Clone ID Related Accession (Same CDS sequence) NM_001352604.1 , XM_017020567.1 , XM_017020563.1 , XM_017020564.1 , XM_017020565.1 , XM_017020566.1
    Accession Version XM_017020564.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876053.1
    CDS225..2996
    Translation

    Target ORF information:

    RefSeq Version XM_017020564.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020564.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84534
    Clone ID Related Accession (Same CDS sequence) NM_001352604.1 , XM_017020567.1 , XM_017020563.1 , XM_017020564.1 , XM_017020565.1 , XM_017020566.1
    Accession Version XM_017020565.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876054.1
    CDS596..3367
    Translation

    Target ORF information:

    RefSeq Version XM_017020565.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020565.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84534
    Clone ID Related Accession (Same CDS sequence) NM_001352604.1 , XM_017020567.1 , XM_017020563.1 , XM_017020564.1 , XM_017020565.1 , XM_017020566.1
    Accession Version XM_017020566.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2772bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876055.1
    CDS499..3270
    Translation

    Target ORF information:

    RefSeq Version XM_017020566.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020566.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGGTAACG GAGCTTGCCC GAGGCCAGAT TTGTGTTCAA CTTTAGAACG AATACAGAAA
    AGTAAAGGAC GTGCAGAAAA AATCAGACAC TTCAGGGAAT TTTTAGATTC TTGGAGAAAA
    TTTCATGATG CTCTTCATAA GAACCACAAA GATGTCACAG ACTCTTTTTA TCCAGCAATG
    AGACTAATTC TTCCTCAGCT AGAAAGAGAG AGAATGGCCT ATGGAATTAA AGAAACTATG
    CTTGCTAAGC TTTATATTGA GTTGCTTAAT TTACCTAGAG ATGGAAAAGA TGCCCTCAAA
    CTTTTAAACT ACAGAACACC CACTGGAACT CATGGAGATG CTGGAGACTT TGCAATGATT
    GCATATTTTG TGTTGAAGCC AAGATGTTTA CAGAAAGGAA GTTTAACCAT ACAGCAAGTA
    AACGACCTTT TAGACTCAAT TGCCAGCAAT AATTCTGCTA AAAGAAAAGA CCTAATAAAA
    AAGAGCCTTC TTCAACTTAT AACTCAGAGT TCAGCACTTG AGCAAAAGTG GCTTATACGG
    ATGATCATAA AGGATTTAAA GCTTGGTGTT AGTCAGCAAA CTATCTTTTC TGTTTTTCAT
    AATGATGCTG CTGAGTTGCA TAATGTCACT ACAGATCTGG AAAAAGTCTG TAGGCAACTG
    CATGATCCTT CTGTAGGACT CAGTGATATT TCTATCACTT TATTTTCTGC ATTTAAACCA
    ATGCTAGCTG CTATTGCAGA TATTGAGCAC ATTGAGAAGG ATATGAAACA TCAGAGTTTC
    TACATAGAAA CCAAGCTAGA TGGTGAACGT ATGCAAATGC ACAAAGATGG AGATGTATAT
    AAATACTTCT CTCGAAATGG ATATAACTAC ACTGATCAGT TTGGTGCTTC TCCTACTGAA
    GGTTCTCTTA CCCCATTCAT TCATAATGCA TTCAAAGCAG ATATACAAAT CTGTATTCTT
    GATGGTGAGA TGATGGCCTA TAATCCTAAT ACACAAACTT TCATGCAAAA GGGAACTAAG
    TTTGATATTA AAAGAATGGT AGAGGATTCT GATCTGCAAA CTTGTTATTG TGTTTTTGAT
    GTATTGATGG TTAATAATAA AAAGCTAGGG CATGAGACTC TGAGAAAGAG GTATGAGATT
    CTTAGTAGTA TTTTTACACC AATTCCAGGT AGAATAGAAA TAGTGCAGAA AACACAAGCT
    CATACTAAGA ATGAAGTAAT TGATGCATTG AATGAAGCAA TAGATAAAAG AGAAGAGGGA
    ATTATGGTAA AACAACCTCT ATCCATCTAC AAGCCAGACA AAAGAGGTGA AGGGTGGTTA
    AAAATTAAAC CAGAGTATGT CAGTGGACTA ATGGATGAAT TGGACATTTT AATTGTTGGA
    GGATATTGGG GTAAAGGATC ACGGGGTGGA ATGATGTCTC ATTTTCTGTG TGCAGTAGCA
    GAGAAGCCCC CTCCTGGTGA GAAGCCATCT GTGTTTCATA CTCTCTCTCG TGTTGGGTCT
    GGCTGCACCA TGAAAGAACT GTATGATCTG GGTTTGAAAT TGGCCAAGTA TTGGAAGCCT
    TTTCATAGAA AAGCTCCACC AAGCAGCATT TTATGTGGAA CAGAGAAGCC AGAAGTATAC
    ATTGAACCTT GTAATTCTGT CATTGTTCAG ATTAAAGCAG CAGAGATCGT ACCCAGTGAT
    ATGTATAAAA CTGGCTGCAC CTTGCGTTTT CCACGAATTG AAAAGATAAG AGATGACAAG
    GAGTGGCATG AGTGCATGAC CCTGGACGAC CTAGAACAAC TTAGGGGGAA GGCATCTGGT
    AAGCTCGCAT CTAAACACCT TTATATAGGT GGTGATGATG AACCACAAGA AAAAAAGCGG
    AAAGCTGCCC CAAAGATGAA GAAAGTTATT GGAATTATTG AGCACTTAAA AGCACCTAAC
    CTTACTAACG TTAACAAAAT TTCTAATATA TTTGAAGATG TAGAGTTTTG TGTTATGAGT
    GGAACAGATA GCCAGCCAAA GCCTGACCTG GAGAACAGAA TTGCAGAATT TGGTGGTTAT
    ATAGTACAAA ATCCAGGCCC AGACACGTAC TGTGTAATTG CAGGGTCTGA GAACATCAGA
    GTGAAAAACA TAATTTTGTC AAATAAACAT GATGTTGTCA AGCCTGCATG GCTTTTAGAA
    TGTTTTAAGA CCAAAAGCTT TGTACCATGG CAGCCTCGCT TTATGATTCA TATGTGCCCA
    TCAACCAAAG AACATTTTGC CCGTGAATAT GATTGCTATG GTGATAGTTA TTTCATTGAT
    ACAGACTTGA ACCAACTGAA GGAAGTATTC TCAGGAATTA AAAATTCTAA CGAGCAGACT
    CCTGAAGAAA TGGCTTCTCT GATTGCTGAT TTAGAATATC GGTATTCCTG GGATTGCTCT
    CCTCTCAGTA TGTTTCGACG CCACACCGTT TATTTGGACT CGTATGCTGT TATTAATGAC
    CTGAGTACCA AAAATGAGGG GACAAGGTTA GCTATTAAAG CCTTGGAGCT TCGGTTTCAT
    GGAGCAAAAG TAGTTTCTTG TTTAGCTGAG GGAGTGTCTC ATGTAATAAT TGGGGAAGAT
    CATAGTCGTG TTGCAGATTT TAAAGCTTTT AGAAGAACTT TTAAGAGAAA GTTTAAAATC
    CTAAAAGAAA GTTGGGTAAC TGATTCAATA GACAAGTGTG AATTACAAGA AGAAAACCAG
    TATTTGATTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84535
    Clone ID Related Accession (Same CDS sequence) XM_017020568.2 , XM_017020568.1
    Accession Version XM_017020568.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017020568.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGATTTGT GTTCAACTTT AGAACGAATA CAGAAAAGTA AAGGACGTGC AGAAAAAATC
    AGACACTTCA GGGAATTTTT AGATTCTTGG AGAAAATTTC ATGATGCTCT TCATAAGAAC
    CACAAAGATG TCACAGACTC TTTTTATCCA GCAATGAGAC TAATTCTTCC TCAGCTAGAA
    AGAGAGAGAA TGGCCTATGG AATTAAAGAA ACTATGCTTG CTAAGCTTTA TATTGAGTTG
    CTTAATTTAC CTAGAGATGG AAAAGATGCC CTCAAACTTT TAAACTACAG AACACCCACT
    GGAACTCATG GAGATGCTGG AGACTTTGCA ATGATTGCAT ATTTTGTGTT GAAGCCAAGA
    TGTTTACAGA AAGGAAGTTT AACCATACAG CAAGTAAACG ACCTTTTAGA CTCAATTGCC
    AGCAATAATT CTGCTAAAAG AAAAGACCTA ATAAAAAAGA GCCTTCTTCA ACTTATAACT
    CAGAGTTCAG CACTTGAGCA AAAGTGGCTT ATACGGATGA TCATAAAGGA TTTAAAGCTT
    GGTGTTAGTC AGCAAACTAT CTTTTCTGTT TTTCATAATG ATGCTGCTGA GTTGCATAAT
    GTCACTACAG ATCTGGAAAA AGTCTGTAGG CAACTGCATG ATCCTTCTGT AGGACTCAGT
    GATATTTCTA TCACTTTATT TTCTGCATTT AAACCAATGC TAGCTGCTAT TGCAGATATT
    GAGCACATTG AGAAGGATAT GAAACATCAG AGTTTCTACA TAGAAACCAA GCTAGATGGT
    GAACGTATGC AAATGCACAA AGATGGAGAT GTATATAAAT ACTTCTCTCG AAATGGATAT
    AACTACACTG ATCAGTTTGG TGCTTCTCCT ACTGAAGGTT CTCTTACCCC ATTCATTCAT
    AATGCATTCA AAGCAGATAT ACAAATCTGT ATTCTTGATG GTGAGATGAT GGCCTATAAT
    CCTAATACAC AAACTTTCAT GCAAAAGGGA ACTAAGTTTG ATATTAAAAG AATGGTAGAG
    GATTCTGATC TGCAAACTTG TTATTGTGTT TTTGATGTAT TGATGGTTAA TAATAAAAAG
    CTAGGGCATG AGACTCTGAG AAAGAGGTAT GAGATTCTTA GTAGTATTTT TACACCAATT
    CCAGGTAGAA TAGAAATAGT GCAGAAAACA CAAGCTCATA CTAAGAATGA AGTAATTGAT
    GCATTGAATG AAGCAATAGA TAAAAGAGAA GAGGGAATTA TGGTAAAACA ACCTCTATCC
    ATCTACAAGC CAGACAAAAG AGGTGAAGGG TGGTTAAAAA TTAAACCAGA GTATGTCAGT
    GGACTAATGG ATGAATTGGA CATTTTAATT GTTGGAGGAT ATTGGGGTAA AGGATCACGG
    GGTGGAATGA TGTCTCATTT TCTGTGTGCA GTAGCAGAGA AGCCCCCTCC TGGTGAGAAG
    CCATCTGTGT TTCATACTCT CTCTCGTGTT GGGTCTGGCT GCACCATGAA AGAACTGTAT
    GATCTGGGTT TGAAATTGGC CAAGTATTGG AAGCCTTTTC ATAGAAAAGC TCCACCAAGC
    AGCATTTTAT GTGGAACAGA GAAGCCAGAA GTATACATTG AACCTTGTAA TTCTGTCATT
    GTTCAGATTA AAGCAGCAGA GATCGTACCC AGTGATATGT ATAAAACTGG CTGCACCTTG
    CGTTTTCCAC GAATTGAAAA GATAAGAGAT GACAAGGAGT GGCATGAGTG CATGACCCTG
    GACGACCTAG AACAACTTAG GGGGAAGGCA TCTGGTAAGC TCGCATCTAA ACACCTTTAT
    ATAGGTGGTG ATGATGAACC ACAAGAAAAA AAGCGGAAAG CTGCCCCAAA GATGAAGAAA
    GTTATTGGAA TTATTGAGCA CTTAAAAGCA CCTAACCTTA CTAACGTTAA CAAAATTTCT
    AATATATTTG AAGATGTAGA GTTTTGTGTT ATGAGTGGAA CAGATAGCCA GCCAAAGCCT
    GACCTGGAGA ACAGAATTGC AGAATTTGGT GGTTATATAG TACAAAATCC AGGCCCAGAC
    ACGTACTGTG TAATTGCAGG GTCTGAGAAC ATCAGAGTGA AAAACATAAT TTTGTCAAAT
    AAACATGATG TTGTCAAGCC TGCATGGCTT TTAGAATGTT TTAAGACCAA AAGCTTTGTA
    CCATGGCAGC CTCGCTTTAT GATTCATATG TGCCCATCAA CCAAAGAACA TTTTGCCCGT
    GAATATGATT GCTATGGTGA TAGTTATTTC ATTGATACAG ACTTGAACCA ACTGAAGGAA
    GTATTCTCAG GAATTAAAAA TTCTAACGAG CAGACTCCTG AAGAAATGGC TTCTCTGATT
    GCTGATTTAG AATATCGGTA TTCCTGGGAT TGCTCTCCTC TCAGTATGTT TCGACGCCAC
    ACCGTTTATT TGGACTCGTA TGCTGTTATT AATGACCTGA GTACCAAAAA TGAGGGGACA
    AGGTTAGCTA TTAAAGCCTT GGAGCTTCGG TTTCATGGAG CAAAAGTAGT TTCTTGTTTA
    GCTGAGGGAG TGTCTCATGT AATAATTGGG GAAGATCATA GTCGTGTTGC AGATTTTAAA
    GCTTTTAGAA GAACTTTTAA GAGAAAGTTT AAAATCCTAA AAGAAAGTTG GGTAACTGAT
    TCAATAGACA AGTGTGAATT ACAAGAAGAA AACCAGTATT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876057.1
    CDS2027..4774
    Translation

    Target ORF information:

    RefSeq Version XM_017020568.2
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020568.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGATTTGT GTTCAACTTT AGAACGAATA CAGAAAAGTA AAGGACGTGC AGAAAAAATC
    AGACACTTCA GGGAATTTTT AGATTCTTGG AGAAAATTTC ATGATGCTCT TCATAAGAAC
    CACAAAGATG TCACAGACTC TTTTTATCCA GCAATGAGAC TAATTCTTCC TCAGCTAGAA
    AGAGAGAGAA TGGCCTATGG AATTAAAGAA ACTATGCTTG CTAAGCTTTA TATTGAGTTG
    CTTAATTTAC CTAGAGATGG AAAAGATGCC CTCAAACTTT TAAACTACAG AACACCCACT
    GGAACTCATG GAGATGCTGG AGACTTTGCA ATGATTGCAT ATTTTGTGTT GAAGCCAAGA
    TGTTTACAGA AAGGAAGTTT AACCATACAG CAAGTAAACG ACCTTTTAGA CTCAATTGCC
    AGCAATAATT CTGCTAAAAG AAAAGACCTA ATAAAAAAGA GCCTTCTTCA ACTTATAACT
    CAGAGTTCAG CACTTGAGCA AAAGTGGCTT ATACGGATGA TCATAAAGGA TTTAAAGCTT
    GGTGTTAGTC AGCAAACTAT CTTTTCTGTT TTTCATAATG ATGCTGCTGA GTTGCATAAT
    GTCACTACAG ATCTGGAAAA AGTCTGTAGG CAACTGCATG ATCCTTCTGT AGGACTCAGT
    GATATTTCTA TCACTTTATT TTCTGCATTT AAACCAATGC TAGCTGCTAT TGCAGATATT
    GAGCACATTG AGAAGGATAT GAAACATCAG AGTTTCTACA TAGAAACCAA GCTAGATGGT
    GAACGTATGC AAATGCACAA AGATGGAGAT GTATATAAAT ACTTCTCTCG AAATGGATAT
    AACTACACTG ATCAGTTTGG TGCTTCTCCT ACTGAAGGTT CTCTTACCCC ATTCATTCAT
    AATGCATTCA AAGCAGATAT ACAAATCTGT ATTCTTGATG GTGAGATGAT GGCCTATAAT
    CCTAATACAC AAACTTTCAT GCAAAAGGGA ACTAAGTTTG ATATTAAAAG AATGGTAGAG
    GATTCTGATC TGCAAACTTG TTATTGTGTT TTTGATGTAT TGATGGTTAA TAATAAAAAG
    CTAGGGCATG AGACTCTGAG AAAGAGGTAT GAGATTCTTA GTAGTATTTT TACACCAATT
    CCAGGTAGAA TAGAAATAGT GCAGAAAACA CAAGCTCATA CTAAGAATGA AGTAATTGAT
    GCATTGAATG AAGCAATAGA TAAAAGAGAA GAGGGAATTA TGGTAAAACA ACCTCTATCC
    ATCTACAAGC CAGACAAAAG AGGTGAAGGG TGGTTAAAAA TTAAACCAGA GTATGTCAGT
    GGACTAATGG ATGAATTGGA CATTTTAATT GTTGGAGGAT ATTGGGGTAA AGGATCACGG
    GGTGGAATGA TGTCTCATTT TCTGTGTGCA GTAGCAGAGA AGCCCCCTCC TGGTGAGAAG
    CCATCTGTGT TTCATACTCT CTCTCGTGTT GGGTCTGGCT GCACCATGAA AGAACTGTAT
    GATCTGGGTT TGAAATTGGC CAAGTATTGG AAGCCTTTTC ATAGAAAAGC TCCACCAAGC
    AGCATTTTAT GTGGAACAGA GAAGCCAGAA GTATACATTG AACCTTGTAA TTCTGTCATT
    GTTCAGATTA AAGCAGCAGA GATCGTACCC AGTGATATGT ATAAAACTGG CTGCACCTTG
    CGTTTTCCAC GAATTGAAAA GATAAGAGAT GACAAGGAGT GGCATGAGTG CATGACCCTG
    GACGACCTAG AACAACTTAG GGGGAAGGCA TCTGGTAAGC TCGCATCTAA ACACCTTTAT
    ATAGGTGGTG ATGATGAACC ACAAGAAAAA AAGCGGAAAG CTGCCCCAAA GATGAAGAAA
    GTTATTGGAA TTATTGAGCA CTTAAAAGCA CCTAACCTTA CTAACGTTAA CAAAATTTCT
    AATATATTTG AAGATGTAGA GTTTTGTGTT ATGAGTGGAA CAGATAGCCA GCCAAAGCCT
    GACCTGGAGA ACAGAATTGC AGAATTTGGT GGTTATATAG TACAAAATCC AGGCCCAGAC
    ACGTACTGTG TAATTGCAGG GTCTGAGAAC ATCAGAGTGA AAAACATAAT TTTGTCAAAT
    AAACATGATG TTGTCAAGCC TGCATGGCTT TTAGAATGTT TTAAGACCAA AAGCTTTGTA
    CCATGGCAGC CTCGCTTTAT GATTCATATG TGCCCATCAA CCAAAGAACA TTTTGCCCGT
    GAATATGATT GCTATGGTGA TAGTTATTTC ATTGATACAG ACTTGAACCA ACTGAAGGAA
    GTATTCTCAG GAATTAAAAA TTCTAACGAG CAGACTCCTG AAGAAATGGC TTCTCTGATT
    GCTGATTTAG AATATCGGTA TTCCTGGGAT TGCTCTCCTC TCAGTATGTT TCGACGCCAC
    ACCGTTTATT TGGACTCGTA TGCTGTTATT AATGACCTGA GTACCAAAAA TGAGGGGACA
    AGGTTAGCTA TTAAAGCCTT GGAGCTTCGG TTTCATGGAG CAAAAGTAGT TTCTTGTTTA
    GCTGAGGGAG TGTCTCATGT AATAATTGGG GAAGATCATA GTCGTGTTGC AGATTTTAAA
    GCTTTTAGAA GAACTTTTAA GAGAAAGTTT AAAATCCTAA AAGAAAGTTG GGTAACTGAT
    TCAATAGACA AGTGTGAATT ACAAGAAGAA AACCAGTATT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu84535
    Clone ID Related Accession (Same CDS sequence) XM_017020568.2 , XM_017020568.1
    Accession Version XM_017020568.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2748bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product DNA ligase 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGATTTGT GTTCAACTTT AGAACGAATA CAGAAAAGTA AAGGACGTGC AGAAAAAATC
    AGACACTTCA GGGAATTTTT AGATTCTTGG AGAAAATTTC ATGATGCTCT TCATAAGAAC
    CACAAAGATG TCACAGACTC TTTTTATCCA GCAATGAGAC TAATTCTTCC TCAGCTAGAA
    AGAGAGAGAA TGGCCTATGG AATTAAAGAA ACTATGCTTG CTAAGCTTTA TATTGAGTTG
    CTTAATTTAC CTAGAGATGG AAAAGATGCC CTCAAACTTT TAAACTACAG AACACCCACT
    GGAACTCATG GAGATGCTGG AGACTTTGCA ATGATTGCAT ATTTTGTGTT GAAGCCAAGA
    TGTTTACAGA AAGGAAGTTT AACCATACAG CAAGTAAACG ACCTTTTAGA CTCAATTGCC
    AGCAATAATT CTGCTAAAAG AAAAGACCTA ATAAAAAAGA GCCTTCTTCA ACTTATAACT
    CAGAGTTCAG CACTTGAGCA AAAGTGGCTT ATACGGATGA TCATAAAGGA TTTAAAGCTT
    GGTGTTAGTC AGCAAACTAT CTTTTCTGTT TTTCATAATG ATGCTGCTGA GTTGCATAAT
    GTCACTACAG ATCTGGAAAA AGTCTGTAGG CAACTGCATG ATCCTTCTGT AGGACTCAGT
    GATATTTCTA TCACTTTATT TTCTGCATTT AAACCAATGC TAGCTGCTAT TGCAGATATT
    GAGCACATTG AGAAGGATAT GAAACATCAG AGTTTCTACA TAGAAACCAA GCTAGATGGT
    GAACGTATGC AAATGCACAA AGATGGAGAT GTATATAAAT ACTTCTCTCG AAATGGATAT
    AACTACACTG ATCAGTTTGG TGCTTCTCCT ACTGAAGGTT CTCTTACCCC ATTCATTCAT
    AATGCATTCA AAGCAGATAT ACAAATCTGT ATTCTTGATG GTGAGATGAT GGCCTATAAT
    CCTAATACAC AAACTTTCAT GCAAAAGGGA ACTAAGTTTG ATATTAAAAG AATGGTAGAG
    GATTCTGATC TGCAAACTTG TTATTGTGTT TTTGATGTAT TGATGGTTAA TAATAAAAAG
    CTAGGGCATG AGACTCTGAG AAAGAGGTAT GAGATTCTTA GTAGTATTTT TACACCAATT
    CCAGGTAGAA TAGAAATAGT GCAGAAAACA CAAGCTCATA CTAAGAATGA AGTAATTGAT
    GCATTGAATG AAGCAATAGA TAAAAGAGAA GAGGGAATTA TGGTAAAACA ACCTCTATCC
    ATCTACAAGC CAGACAAAAG AGGTGAAGGG TGGTTAAAAA TTAAACCAGA GTATGTCAGT
    GGACTAATGG ATGAATTGGA CATTTTAATT GTTGGAGGAT ATTGGGGTAA AGGATCACGG
    GGTGGAATGA TGTCTCATTT TCTGTGTGCA GTAGCAGAGA AGCCCCCTCC TGGTGAGAAG
    CCATCTGTGT TTCATACTCT CTCTCGTGTT GGGTCTGGCT GCACCATGAA AGAACTGTAT
    GATCTGGGTT TGAAATTGGC CAAGTATTGG AAGCCTTTTC ATAGAAAAGC TCCACCAAGC
    AGCATTTTAT GTGGAACAGA GAAGCCAGAA GTATACATTG AACCTTGTAA TTCTGTCATT
    GTTCAGATTA AAGCAGCAGA GATCGTACCC AGTGATATGT ATAAAACTGG CTGCACCTTG
    CGTTTTCCAC GAATTGAAAA GATAAGAGAT GACAAGGAGT GGCATGAGTG CATGACCCTG
    GACGACCTAG AACAACTTAG GGGGAAGGCA TCTGGTAAGC TCGCATCTAA ACACCTTTAT
    ATAGGTGGTG ATGATGAACC ACAAGAAAAA AAGCGGAAAG CTGCCCCAAA GATGAAGAAA
    GTTATTGGAA TTATTGAGCA CTTAAAAGCA CCTAACCTTA CTAACGTTAA CAAAATTTCT
    AATATATTTG AAGATGTAGA GTTTTGTGTT ATGAGTGGAA CAGATAGCCA GCCAAAGCCT
    GACCTGGAGA ACAGAATTGC AGAATTTGGT GGTTATATAG TACAAAATCC AGGCCCAGAC
    ACGTACTGTG TAATTGCAGG GTCTGAGAAC ATCAGAGTGA AAAACATAAT TTTGTCAAAT
    AAACATGATG TTGTCAAGCC TGCATGGCTT TTAGAATGTT TTAAGACCAA AAGCTTTGTA
    CCATGGCAGC CTCGCTTTAT GATTCATATG TGCCCATCAA CCAAAGAACA TTTTGCCCGT
    GAATATGATT GCTATGGTGA TAGTTATTTC ATTGATACAG ACTTGAACCA ACTGAAGGAA
    GTATTCTCAG GAATTAAAAA TTCTAACGAG CAGACTCCTG AAGAAATGGC TTCTCTGATT
    GCTGATTTAG AATATCGGTA TTCCTGGGAT TGCTCTCCTC TCAGTATGTT TCGACGCCAC
    ACCGTTTATT TGGACTCGTA TGCTGTTATT AATGACCTGA GTACCAAAAA TGAGGGGACA
    AGGTTAGCTA TTAAAGCCTT GGAGCTTCGG TTTCATGGAG CAAAAGTAGT TTCTTGTTTA
    GCTGAGGGAG TGTCTCATGT AATAATTGGG GAAGATCATA GTCGTGTTGC AGATTTTAAA
    GCTTTTAGAA GAACTTTTAA GAGAAAGTTT AAAATCCTAA AAGAAAGTTG GGTAACTGAT
    TCAATAGACA AGTGTGAATT ACAAGAAGAA AACCAGTATT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016876057.1
    CDS1799..4546
    Translation

    Target ORF information:

    RefSeq Version XM_017020568.1
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA ligase 4 (LIG4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017020568.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGCAACCC CGCAGGAAAC CATCAAGATC TCATTTTACA GCTGGGATTC TCTGGTTCAC 
    AGAGATTTGT GTTCAACTTT AGAACGAATA CAGAAAAGTA AAGGACGTGC AGAAAAAATC
    AGACACTTCA GGGAATTTTT AGATTCTTGG AGAAAATTTC ATGATGCTCT TCATAAGAAC
    CACAAAGATG TCACAGACTC TTTTTATCCA GCAATGAGAC TAATTCTTCC TCAGCTAGAA
    AGAGAGAGAA TGGCCTATGG AATTAAAGAA ACTATGCTTG CTAAGCTTTA TATTGAGTTG
    CTTAATTTAC CTAGAGATGG AAAAGATGCC CTCAAACTTT TAAACTACAG AACACCCACT
    GGAACTCATG GAGATGCTGG AGACTTTGCA ATGATTGCAT ATTTTGTGTT GAAGCCAAGA
    TGTTTACAGA AAGGAAGTTT AACCATACAG CAAGTAAACG ACCTTTTAGA CTCAATTGCC
    AGCAATAATT CTGCTAAAAG AAAAGACCTA ATAAAAAAGA GCCTTCTTCA ACTTATAACT
    CAGAGTTCAG CACTTGAGCA AAAGTGGCTT ATACGGATGA TCATAAAGGA TTTAAAGCTT
    GGTGTTAGTC AGCAAACTAT CTTTTCTGTT TTTCATAATG ATGCTGCTGA GTTGCATAAT
    GTCACTACAG ATCTGGAAAA AGTCTGTAGG CAACTGCATG ATCCTTCTGT AGGACTCAGT
    GATATTTCTA TCACTTTATT TTCTGCATTT AAACCAATGC TAGCTGCTAT TGCAGATATT
    GAGCACATTG AGAAGGATAT GAAACATCAG AGTTTCTACA TAGAAACCAA GCTAGATGGT
    GAACGTATGC AAATGCACAA AGATGGAGAT GTATATAAAT ACTTCTCTCG AAATGGATAT
    AACTACACTG ATCAGTTTGG TGCTTCTCCT ACTGAAGGTT CTCTTACCCC ATTCATTCAT
    AATGCATTCA AAGCAGATAT ACAAATCTGT ATTCTTGATG GTGAGATGAT GGCCTATAAT
    CCTAATACAC AAACTTTCAT GCAAAAGGGA ACTAAGTTTG ATATTAAAAG AATGGTAGAG
    GATTCTGATC TGCAAACTTG TTATTGTGTT TTTGATGTAT TGATGGTTAA TAATAAAAAG
    CTAGGGCATG AGACTCTGAG AAAGAGGTAT GAGATTCTTA GTAGTATTTT TACACCAATT
    CCAGGTAGAA TAGAAATAGT GCAGAAAACA CAAGCTCATA CTAAGAATGA AGTAATTGAT
    GCATTGAATG AAGCAATAGA TAAAAGAGAA GAGGGAATTA TGGTAAAACA ACCTCTATCC
    ATCTACAAGC CAGACAAAAG AGGTGAAGGG TGGTTAAAAA TTAAACCAGA GTATGTCAGT
    GGACTAATGG ATGAATTGGA CATTTTAATT GTTGGAGGAT ATTGGGGTAA AGGATCACGG
    GGTGGAATGA TGTCTCATTT TCTGTGTGCA GTAGCAGAGA AGCCCCCTCC TGGTGAGAAG
    CCATCTGTGT TTCATACTCT CTCTCGTGTT GGGTCTGGCT GCACCATGAA AGAACTGTAT
    GATCTGGGTT TGAAATTGGC CAAGTATTGG AAGCCTTTTC ATAGAAAAGC TCCACCAAGC
    AGCATTTTAT GTGGAACAGA GAAGCCAGAA GTATACATTG AACCTTGTAA TTCTGTCATT
    GTTCAGATTA AAGCAGCAGA GATCGTACCC AGTGATATGT ATAAAACTGG CTGCACCTTG
    CGTTTTCCAC GAATTGAAAA GATAAGAGAT GACAAGGAGT GGCATGAGTG CATGACCCTG
    GACGACCTAG AACAACTTAG GGGGAAGGCA TCTGGTAAGC TCGCATCTAA ACACCTTTAT
    ATAGGTGGTG ATGATGAACC ACAAGAAAAA AAGCGGAAAG CTGCCCCAAA GATGAAGAAA
    GTTATTGGAA TTATTGAGCA CTTAAAAGCA CCTAACCTTA CTAACGTTAA CAAAATTTCT
    AATATATTTG AAGATGTAGA GTTTTGTGTT ATGAGTGGAA CAGATAGCCA GCCAAAGCCT
    GACCTGGAGA ACAGAATTGC AGAATTTGGT GGTTATATAG TACAAAATCC AGGCCCAGAC
    ACGTACTGTG TAATTGCAGG GTCTGAGAAC ATCAGAGTGA AAAACATAAT TTTGTCAAAT
    AAACATGATG TTGTCAAGCC TGCATGGCTT TTAGAATGTT TTAAGACCAA AAGCTTTGTA
    CCATGGCAGC CTCGCTTTAT GATTCATATG TGCCCATCAA CCAAAGAACA TTTTGCCCGT
    GAATATGATT GCTATGGTGA TAGTTATTTC ATTGATACAG ACTTGAACCA ACTGAAGGAA
    GTATTCTCAG GAATTAAAAA TTCTAACGAG CAGACTCCTG AAGAAATGGC TTCTCTGATT
    GCTGATTTAG AATATCGGTA TTCCTGGGAT TGCTCTCCTC TCAGTATGTT TCGACGCCAC
    ACCGTTTATT TGGACTCGTA TGCTGTTATT AATGACCTGA GTACCAAAAA TGAGGGGACA
    AGGTTAGCTA TTAAAGCCTT GGAGCTTCGG TTTCATGGAG CAAAAGTAGT TTCTTGTTTA
    GCTGAGGGAG TGTCTCATGT AATAATTGGG GAAGATCATA GTCGTGTTGC AGATTTTAAA
    GCTTTTAGAA GAACTTTTAA GAGAAAGTTT AAAATCCTAA AAGAAAGTTG GGTAACTGAT
    TCAATAGACA AGTGTGAATT ACAAGAAGAA AACCAGTATT TGATTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.