LIG3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	LIG3
Entrez Gene ID	3980
Full Name	DNA ligase 3
Synonyms	LIG2
General protein information	Preferred Names DNA ligase 3 Names DNA ligase 3 ligase II, DNA, ATP-dependent ligase III, DNA, ATP-dependent polydeoxyribonucleotide synthase [ATP] 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q12
Summary	This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	600940

mRNA and Protein(s)

mRNA	Protein	Name
NM_013975.4	NP_039269.2	DNA ligase 3 isoform alpha precursor
XM_017024624.1	XP_016880113.1	DNA ligase 3 isoform X2
NM_002311.4	NP_002302.2	DNA ligase 3 isoform beta precursor
XM_005257970.3	XP_005258027.1	DNA ligase 3 isoform X1
NM_013975.3	NP_039269.2	DNA ligase 3 isoform alpha precursor
XM_006721896.3	XP_006721959.1	DNA ligase 3 isoform X3
XM_005257970.4	XP_005258027.1	DNA ligase 3 isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	LIG3	NP_001033196.1
Rattus norvegicus (Norway rat)	Lig3	NP_001012011.1
Gallus gallus (chicken)	LIG3	NP_001006215.1
Homo sapiens (human)	LIG3	NP_039269.2
	LIG3	XP_001113780.1
Canis lupus familiaris (dog)	LIG3	XP_005624869.1
Mus musculus (house mouse)	Lig3	NP_034846.2
Drosophila melanogaster (fruit fly)	lig3	NP_650187.2
Xenopus tropicalis (tropical clawed frog)	lig3	NP_001016351.2
Pan troglodytes (chimpanzee)	LIG3	XP_511409.3
Danio rerio (zebrafish)	lig3	NP_001025345.2

Gene Ontology
Bibliography

Related articles in PubMed

Kinetic analyses of single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase I.
McNally JR, O'Brien PJ
The Journal of biological chemistry292(38)15870-15879(2017 09)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase I
Title: Kinetic analyses of single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase I.

A computational approach to determine susceptibility to cancer by evaluating the deleterious effect of nsSNP in XRCC1 gene on binding interaction of XRCC1 protein with ligase III.
Title: A computational approach to determine susceptibility to cancer by evaluating the deleterious effect of nsSNP in XRCC1 gene on binding interaction of XRCC1 protein with ligase III.

Domains constituting the LigIII catalytic core collaborate and are essential for formation of a DNA-bridging intermediate by adenylated LigIII that positions a pair of blunt-ended duplex DNAs for efficient and specific intermolecular ligation.
Title: Human DNA ligase III bridges two DNA ends to promote specific intermolecular DNA end joining.

In the context of tyrosine kinase-activated leukemias, c-MYC contributes to aberrant DNA repair through downstream targets LIG3 and PARP1 up-regulation.
Title: c-MYC Generates Repair Errors via Increased Transcription of Alternative-NHEJ Factors, LIG3 and PARP1, in Tyrosine Kinase-Activated Leukemias.

The g.29661G>A and g.29059C>T polymorphisms of LIG3 may play a role in the keratoconus and Fuchs endothelial corneal dystrophy pathogenesis and can be considered as markers in these diseases.
Title: Polymorphism of the LIG3 gene in keratoconus and Fuchs endothelial corneal dystrophy.

The following LIG3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LIG3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18231	NM_013975.4 Latest version!	Homo sapiens DNA ligase 3 (LIG3), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu18231	XM_017024624.1 Latest version!	Homo sapiens DNA ligase 3 (LIG3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu09436	NM_002311.4 Latest version!	Homo sapiens DNA ligase 3 (LIG3), transcript variant beta, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu50375	XM_006721896.3 Latest version!	Homo sapiens DNA ligase 3 (LIG3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu50374	XM_005257970.4 Latest version!	Homo sapiens DNA ligase 3 (LIG3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
View Old Accession Versions >>
OHu18231	NM_013975.3	Homo sapiens DNA ligase 3 (LIG3), transcript variant alpha, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu50374	XM_005257970.3	Homo sapiens DNA ligase 3 (LIG3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LIG3 ( NM_013975.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_039269.2 Homo sapiens DNA ligase 3 (LIG3), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu18231
Clone ID Related Accession (Same CDS sequence)	NM_013975.4 , XM_017024624.1 , NM_013975.3
Accession Version	NM_013975.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-20
Organism	Homo sapiens(human)
Product	DNA ligase 3 isoform alpha precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB100562.1, BC068005.1, BM726768.1, AW166526.1, AA872458.1 and AC004223.2. On May 17, 2019 this sequence version replaced NM_013975.3. Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.177858.1, SRR1803611.130782.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000378526.9/ ENSP00000367787.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGGTAT TGCTGGACAT CTTCACTGGG 
GTGCGGCTTT ACTTGCCACC CTCCACACCA GACTTCAGCC GTCTCAGACG CTACTTTGTG 
GCATTCGACG GGGACCTGGT ACAGGAATTT GATATGACTT CAGCCACGCA CGTGCTGGGT 
AGCAGGGACA AGAACCCTGC GGCCCAGCAG GTCTCCCCAG AGTGGATTTG GGCATGTATC 
CGGAAACGGA GACTGGTAGC TCCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_039269.2
CDS	116..3145
Translation	MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	NM_013975.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA ligase 3 (LIG3), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013975.4

ORF Insert Sequence:

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGGTAT TGCTGGACAT CTTCACTGGG 
GTGCGGCTTT ACTTGCCACC CTCCACACCA GACTTCAGCC GTCTCAGACG CTACTTTGTG 
GCATTCGACG GGGACCTGGT ACAGGAATTT GATATGACTT CAGCCACGCA CGTGCTGGGT 
AGCAGGGACA AGAACCCTGC GGCCCAGCAG GTCTCCCCAG AGTGGATTTG GGCATGTATC 
CGGAAACGGA GACTGGTAGC TCCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( NM_013975.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_039269.2 Homo sapiens DNA ligase 3 (LIG3), transcript variant alpha, mRNA.

Price & Availability↑

CloneID	OHu18231
Clone ID Related Accession (Same CDS sequence)	NM_013975.4 , XM_017024624.1 , NM_013975.3
Accession Version	NM_013975.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	DNA ligase 3 isoform alpha precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB100562.1, BC068005.1, BM726768.1, AW166526.1 and AA872458.1. This sequence is a reference standard in the RefSeqGene project. On Jun 29, 2010 this sequence version replaced NM_013975.2. Transcript Variant: This variant (alpha) represents the longer transcript and encodes the longer isoform (alpha). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.148780.1, SRR1660803.177858.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGGTAT TGCTGGACAT CTTCACTGGG 
GTGCGGCTTT ACTTGCCACC CTCCACACCA GACTTCAGCC GTCTCAGACG CTACTTTGTG 
GCATTCGACG GGGACCTGGT ACAGGAATTT GATATGACTT CAGCCACGCA CGTGCTGGGT 
AGCAGGGACA AGAACCCTGC GGCCCAGCAG GTCTCCCCAG AGTGGATTTG GGCATGTATC 
CGGAAACGGA GACTGGTAGC TCCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_039269.2
CDS	130..3159
Translation	MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	NM_013975.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA ligase 3 (LIG3), transcript variant alpha, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_013975.3

ORF Insert Sequence:

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGGTAT TGCTGGACAT CTTCACTGGG 
GTGCGGCTTT ACTTGCCACC CTCCACACCA GACTTCAGCC GTCTCAGACG CTACTTTGTG 
GCATTCGACG GGGACCTGGT ACAGGAATTT GATATGACTT CAGCCACGCA CGTGCTGGGT 
AGCAGGGACA AGAACCCTGC GGCCCAGCAG GTCTCCCCAG AGTGGATTTG GGCATGTATC 
CGGAAACGGA GACTGGTAGC TCCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( XM_017024624.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016880113.1 Homo sapiens DNA ligase 3 (LIG3), transcript variant X2, mRNA.

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CloneID	OHu18231
Clone ID Related Accession (Same CDS sequence)	NM_013975.4 , XM_017024624.1 , NM_013975.3
Accession Version	XM_017024624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3030bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA ligase 3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGGTAT TGCTGGACAT CTTCACTGGG 
GTGCGGCTTT ACTTGCCACC CTCCACACCA GACTTCAGCC GTCTCAGACG CTACTTTGTG 
GCATTCGACG GGGACCTGGT ACAGGAATTT GATATGACTT CAGCCACGCA CGTGCTGGGT 
AGCAGGGACA AGAACCCTGC GGCCCAGCAG GTCTCCCCAG AGTGGATTTG GGCATGTATC 
CGGAAACGGA GACTGGTAGC TCCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016880113.1
CDS	294..3323
Translation	MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_017024624.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA ligase 3 (LIG3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017024624.1

ORF Insert Sequence:

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGGTAT TGCTGGACAT CTTCACTGGG 
GTGCGGCTTT ACTTGCCACC CTCCACACCA GACTTCAGCC GTCTCAGACG CTACTTTGTG 
GCATTCGACG GGGACCTGGT ACAGGAATTT GATATGACTT CAGCCACGCA CGTGCTGGGT 
AGCAGGGACA AGAACCCTGC GGCCCAGCAG GTCTCCCCAG AGTGGATTTG GGCATGTATC 
CGGAAACGGA GACTGGTAGC TCCCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( NM_002311.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002302.2 Homo sapiens DNA ligase 3 (LIG3), transcript variant beta, mRNA.

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CloneID	OHu09436
Clone ID Related Accession (Same CDS sequence)	NM_002311.4
Accession Version	NM_002311.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	DNA ligase 3 isoform beta precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB100562.1, U40671.1 and BG470103.1. On Jun 29, 2010 this sequence version replaced NM_002311.3. Transcript Variant: This variant (beta) contains an alternate in-frame exon in the coding region which includes an in-frame stop codon, compared to variant alpha. This isoform (beta) is shorter and has a distinct C-terminus, compared to isoform alpha. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U40671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGAGGC GGCCAGCCAG TGAGCAGAGA 
GGAAGAACTG TGCCAGCAGG CAGGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002302.2
CDS	130..2979
Translation	MSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKRRPASEQRGRTVPAGRR

Target ORF information:

RefSeq Version	NM_002311.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA ligase 3 (LIG3), transcript variant beta, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002311.4

ORF Insert Sequence:

ATGTCTTTGG CTTTCAAGAT CTTCTTTCCA CAAACCCTCC GTGCACTCAG CCGAAAAGAA 
CTGTGCCTAT TCCGAAAACA TCACTGGCGT GATGTAAGAC AATTCAGCCA GTGGTCAGAA 
ACAGATCTGC TTCATGGACA TCCCCTCTTC CTGAGAAGAA AGCCTGTTCT ATCATTCCAG 
GGAAGCCATC TAAGATCACG TGCCACCTAC CTTGTTTTCT TGCCAGGGTT GCATGTGGGA 
CTCTGCAGTG GCCCCTGTGA GATGGCTGAG CAACGGTTCT GTGTGGACTA TGCCAAGCGT 
GGCACAGCTG GCTGCAAAAA ATGCAAGGAA AAGATTGTGA AGGGCGTATG CCGAATTGGC 
AAAGTGGTGC CCAATCCCTT CTCAGAGTCT GGGGGTGATA TGAAAGAGTG GTACCACATT 
AAATGCATGT TTGAGAAACT AGAGCGGGCC CGGGCCACCA CAAAAAAAAT CGAGGACCTC 
ACAGAGCTGG AAGGCTGGGA AGAGCTGGAA GATAATGAGA AGGAACAGAT AACCCAGCAC 
ATTGCAGATC TGTCTTCTAA GGCAGCAGGT ACACCAAAGA AGAAAGCTGT TGTCCAGGCT 
AAGTTGACAA CCACTGGCCA GGTGACTTCT CCAGTGAAAG GCGCCTCATT TGTCACCAGT 
ACCAATCCCC GGAAATTTTC TGGCTTTTCA GCCAAGCCCA ACAACTCTGG GGAAGCCCCC 
TCGAGCCCCA CCCCTAAGAG AAGTCTGTCT TCAAGCAAAT GTGACCCCAG GCATAAGGAC 
TGTCTGCTAC GGGAGTTTCG AAAGTTATGC GCCATGGTGG CCGATAATCC TAGCTACAAC 
ACGAAGACCC AGATCATCCA GGACTTCCTT CGGAAAGGCT CAGCAGGAGA TGGTTTCCAC 
GGTGATGTGT ACCTAACAGT GAAGCTGCTG CTGCCAGGAG TCATTAAGAC TGTTTACAAC 
TTGAACGATA AGCAGATTGT GAAGCTTTTC AGTCGCATTT TTAACTGCAA CCCAGATGAT 
ATGGCACGGG ACCTAGAGCA GGGTGACGTG TCAGAGACAA TCAGAGTCTT CTTTGAGCAG 
AGCAAGTCTT TCCCCCCAGC TGCCAAGAGC CTCCTTACCA TCCAGGAAGT GGATGAGTTC 
CTTCTGCGGC TGTCCAAGCT CACCAAGGAG GATGAGCAGC AACAGGCCCT ACAGGACATT 
GCCTCCAGGT GTACAGCCAA TGACCTTAAA TGCATCATCA GGTTGATCAA ACATGATCTG 
AAGATGAACT CAGGTGCAAA ACATGTGTTA GACGCCCTTG ACCCCAATGC CTATGAAGCC 
TTCAAAGCCT CGCGCAACCT GCAGGATGTG GTGGAGCGGG TCCTTCACAA CGCGCAGGAG 
GTGGAGAAGG AGCCGGGCCA GAGACGAGCT CTGAGCGTCC AGGCCTCGCT GATGACACCT 
GTGCAGCCCA TGTTGGCGGA GGCCTGCAAG TCCGTTGAGT ATGCAATGAA GAAATGTCCC 
AATGGCATGT TCTCTGAGAT CAAGTACGAT GGAGAGCGAG TCCAGGTGCA TAAGAATGGA 
GACCACTTCA GCTACTTCAG CCGCAGTCTC AAGCCCGTCC TTCCTCACAA GGTGGCCCAC 
TTTAAGGACT ACATTCCCCA GGCTTTTCCT GGGGGCCACA GCATGATCTT GGATTCTGAA 
GTGCTTCTGA TTGACAACAA GACAGGCAAA CCACTGCCCT TTGGGACTCT GGGAGTACAC 
AAGAAAGCAG CCTTCCAGGA TGCTAATGTC TGCCTGTTTG TTTTTGATTG TATCTACTTT 
AATGATGTCA GCTTGATGGA CAGACCTCTG TGTGAGCGGC GGAAGTTTCT TCATGACAAC 
ATGGTTGAAA TTCCAAACCG GATCATGTTC TCAGAAATGA AGCGAGTCAC AAAAGCTTTG 
GACTTGGCTG ACATGATAAC CCGGGTGATC CAGGAGGGAT TGGAGGGGCT GGTGCTGAAG 
GATGTGAAGG GTACATATGA GCCTGGGAAG CGGCACTGGC TGAAAGTGAA GAAAGACTAT 
TTGAACGAGG GGGCCATGGC CGACACAGCT GACCTGGTGG TCCTTGGAGC CTTCTATGGG 
CAAGGGAGCA AAGGCGGCAT GATGTCAATC TTCCTCATGG GCTGCTACGA CCCTGGCAGC 
CAGAAGTGGT GCACAGTCAC CAAGTGTGCA GGAGGCCATG ATGATGCCAC GCTTGCCCGC 
CTGCAGAATG AACTAGACAT GGTGAAGATC AGCAAGGACC CCAGCAAAAT ACCCAGCTGG 
TTGAAGGTCA ACAAGATCTA CTATCCTGAC TTCATCGTCC CAGACCCAAA GAAAGCTGCC 
GTGTGGGAGA TCACAGGGGC TGAATTCTCC AAATCGGAGG CTCATACAGC TGACGGGATC 
TCCATCCGAT TCCCTCGCTG CACCCGAATC CGAGATGATA AGGACTGGAA ATCTGCCACT 
AACCTTCCCC AACTCAAGGA ACTGTACCAG TTGTCCAAGG AGAAGGCAGA CTTCACTGTA 
GTGGCTGGAG ATGAGGGGAG CTCCACTACA GGGGGTAGCA GTGAAGAGAA TAAGGGTCCC 
TCAGGGTCTG CTGTGTCCCG CAAGGCCCCC AGCAAGCCCT CAGCCAGTAC CAAGAAAGCA 
GAAGGGAAGC TGAGTAACTC CAACAGCAAA GATGGCAACA TGCAGACTGC AAAGCCTTCC 
GCTATGAAGG TGGGGGAGAA GCTGGCCACA AAGTCTTCTC CAGTGAAAGT AGGGGAGAAG 
CGGAAAGCTG CTGATGAGAC GCTGTGCCAA ACAAAGAGGC GGCCAGCCAG TGAGCAGAGA 
GGAAGAACTG TGCCAGCAGG CAGGAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( XM_006721896.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006721959.1 Homo sapiens DNA ligase 3 (LIG3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu50375
Clone ID Related Accession (Same CDS sequence)	XM_006721896.3
Accession Version	XM_006721896.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA ligase 3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006721896.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAAGT TCCGAGGCCT ACGCTATATG TCTTTGGCTT TCAAGATCTT CTTTCCACAA 
ACCCTCCGTG CACTCAGCCG AAAAGAACTG TGCCTATTCC GAAAACATCA CTGGCGTGAT 
GTAAGACAAT TCAGCCAGTG GTCAGAAACA GATCTGCTTC ATGGACATCC CCTCTTCCTG 
AGAAGAAAGC CTGTTCTATC ATTCCAGGGA AGCCATCTAA GATCACGTGC CACCTACCTT 
GTTTTCTTGC CAGGGTTGCA TGTGGGACTC TGCAGTGGCC CCTGTGAGAT GGCTGAGCAA 
CGGTTCTGTG TGGACTATGC CAAGCGTGGC ACAGCTGGCT GCAAAAAATG CAAGGAAAAG 
ATTGTGAAGG GCGTATGCCG AATTGGCAAA GTGGTGCCCA ATCCCTTCTC AGAGTCTGGG 
GGTGATATGA AAGAGTGGTA CCACATTAAA TGCATGTTTG AGAAACTAGA GCGGGCCCGG 
GCCACCACAA AAAAAATCGA GGACCTCACA GAGCTGGAAG GCTGGGAAGA GCTGGAAGAT 
AATGAGAAGG AACAGATAAC CCAGCACATT GCAGATCTGT CTTCTAAGGC AGCAGGTACA 
CCAAAGAAGA AAGCTGTTGT CCAGGCTAAG TTGACAACCA CTGGCCAGGT GACTTCTCCA 
GTGAAAGGCG CCTCATTTGT CACCAGTACC AATCCCCGGA AATTTTCTGG CTTTTCAGCC 
AAGCCCAACA ACTCTGGGGA AGCCCCCTCG AGCCCCACCC CTAAGAGAAG TCTGTCTTCA 
AGCAAATGTG ACCCCAGGCA TAAGGACTGT CTGCTACGGG AGTTTCGAAA GTTATGCGCC 
ATGGTGGCCG ATAATCCTAG CTACAACACG AAGACCCAGA TCATCCAGGA CTTCCTTCGG 
AAAGGCTCAG CAGGAGATGG TTTCCACGGT GATGTGTACC TAACAGTGAA GCTGCTGCTG 
CCAGGAGTCA TTAAGACTGT TTACAACTTG AACGATAAGC AGATTGTGAA GCTTTTCAGT 
CGCATTTTTA ACTGCAACCC AGATGATATG GCACGGGACC TAGAGCAGGG TGACGTGTCA 
GAGACAATCA GAGTCTTCTT TGAGCAGAGC AAGTCTTTCC CCCCAGCTGC CAAGAGCCTC 
CTTACCATCC AGGAAGTGGA TGAGTTCCTT CTGCGGCTGT CCAAGCTCAC CAAGGAGGAT 
GAGCAGCAAC AGGCCCTACA GGACATTGCC TCCAGGTGTA CAGCCAATGA CCTTAAATGC 
ATCATCAGGT TGATCAAACA TGATCTGAAG ATGAACTCAG GTGCAAAACA TGTGTTAGAC 
GCCCTTGACC CCAATGCCTA TGAAGCCTTC AAAGCCTCGC GCAACCTGCA GGATGTGGTG 
GAGCGGGTCC TTCACAACGC GCAGGAGGTG GAGAAGGAGC CGGGCCAGAG ACGAGCTCTG 
AGCGTCCAGG CCTCGCTGAT GACACCTGTG CAGCCCATGT TGGCGGAGGC CTGCAAGTCC 
GTTGAGTATG CAATGAAGAA ATGTCCCAAT GGCATGTTCT CTGAGATCAA GTACGATGGA 
GAGCGAGTCC AGGTGCATAA GAATGGAGAC CACTTCAGCT ACTTCAGCCG CAGTCTCAAG 
CCCGTCCTTC CTCACAAGGT GGCCCACTTT AAGGACTACA TTCCCCAGGC TTTTCCTGGG 
GGCCACAGCA TGATCTTGGA TTCTGAAGTG CTTCTGATTG ACAACAAGAC AGGCAAACCA 
CTGCCCTTTG GGACTCTGGG AGTACACAAG AAAGCAGCCT TCCAGGATGC TAATGTCTGC 
CTGTTTGTTT TTGATTGTAT CTACTTTAAT GATGTCAGCT TGATGGACAG ACCTCTGTGT 
GAGCGGCGGA AGTTTCTTCA TGACAACATG GTTGAAATTC CAAACCGGAT CATGTTCTCA 
GAAATGAAGC GAGTCACAAA AGCTTTGGAC TTGGCTGACA TGATAACCCG GGTGATCCAG 
GAGGGATTGG AGGGGCTGGT GCTGAAGGAT GTGAAGGGTA CATATGAGCC TGGGAAGCGG 
CACTGGCTGA AAGTGAAGAA AGACTATTTG AACGAGGGGG CCATGGCCGA CACAGCTGAC 
CTGGTGGTCC TTGGAGCCTT CTATGGGCAA GGGAGCAAAG GCGGCATGAT GTCAATCTTC 
CTCATGGGCT GCTACGACCC TGGCAGCCAG AAGTGGTGCA CAGTCACCAA GTGTGCAGGA 
GGCCATGATG ATGCCACGCT TGCCCGCCTG CAGAATGAAC TAGACATGGT GAAGATCAGC 
AAGGACCCCA GCAAAATACC CAGCTGGTTG AAGGTCAACA AGATCTACTA TCCTGACTTC 
ATCGTCCCAG ACCCAAAGAA AGCTGCCGTG TGGGAGATCA CAGGGGCTGA ATTCTCCAAA 
TCGGAGGCTC ATACAGCTGA CGGGATCTCC ATCCGATTCC CTCGCTGCAC CCGAATCCGA 
GATGATAAGG ACTGGAAATC TGCCACTAAC CTTCCCCAAC TCAAGGAACT GTACCAGTTG 
TCCAAGGAGA AGGCAGACTT CACTGTAGTG GCTGGAGATG AGGGGAGCTC CACTACAGGG 
GGTAGCAGTG AAGAGAATAA GGGTCCCTCA GGGTCTGCTG TGTCCCGCAA GGCCCCCAGC 
AAGCCCTCAG CCAGTACCAA GAAAGCAGAA GGGAAGCTGA GTAACTCCAA CAGCAAAGAT 
GGCAACATGC AGACTGCAAA GCCTTCCGCT ATGAAGGTGG GGGAGAAGCT GGCCACAAAG 
TCTTCTCCAG TGAAAGTAGG GGAGAAGCGG AAAGCTGCTG ATGAGACGCT GTGCCAAACA 
AAGAGGCGGC CAGCCAGTGA GCAGAGAGGA AGAACTGTGC CAGCAGGCAG GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006721959.1
CDS	72..2948
Translation	MSKFRGLRYMSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKRRPASEQRGRTVPAGRR

Target ORF information:

RefSeq Version	XM_006721896.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA ligase 3 (LIG3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006721896.3

ORF Insert Sequence:

ATGAGCAAGT TCCGAGGCCT ACGCTATATG TCTTTGGCTT TCAAGATCTT CTTTCCACAA 
ACCCTCCGTG CACTCAGCCG AAAAGAACTG TGCCTATTCC GAAAACATCA CTGGCGTGAT 
GTAAGACAAT TCAGCCAGTG GTCAGAAACA GATCTGCTTC ATGGACATCC CCTCTTCCTG 
AGAAGAAAGC CTGTTCTATC ATTCCAGGGA AGCCATCTAA GATCACGTGC CACCTACCTT 
GTTTTCTTGC CAGGGTTGCA TGTGGGACTC TGCAGTGGCC CCTGTGAGAT GGCTGAGCAA 
CGGTTCTGTG TGGACTATGC CAAGCGTGGC ACAGCTGGCT GCAAAAAATG CAAGGAAAAG 
ATTGTGAAGG GCGTATGCCG AATTGGCAAA GTGGTGCCCA ATCCCTTCTC AGAGTCTGGG 
GGTGATATGA AAGAGTGGTA CCACATTAAA TGCATGTTTG AGAAACTAGA GCGGGCCCGG 
GCCACCACAA AAAAAATCGA GGACCTCACA GAGCTGGAAG GCTGGGAAGA GCTGGAAGAT 
AATGAGAAGG AACAGATAAC CCAGCACATT GCAGATCTGT CTTCTAAGGC AGCAGGTACA 
CCAAAGAAGA AAGCTGTTGT CCAGGCTAAG TTGACAACCA CTGGCCAGGT GACTTCTCCA 
GTGAAAGGCG CCTCATTTGT CACCAGTACC AATCCCCGGA AATTTTCTGG CTTTTCAGCC 
AAGCCCAACA ACTCTGGGGA AGCCCCCTCG AGCCCCACCC CTAAGAGAAG TCTGTCTTCA 
AGCAAATGTG ACCCCAGGCA TAAGGACTGT CTGCTACGGG AGTTTCGAAA GTTATGCGCC 
ATGGTGGCCG ATAATCCTAG CTACAACACG AAGACCCAGA TCATCCAGGA CTTCCTTCGG 
AAAGGCTCAG CAGGAGATGG TTTCCACGGT GATGTGTACC TAACAGTGAA GCTGCTGCTG 
CCAGGAGTCA TTAAGACTGT TTACAACTTG AACGATAAGC AGATTGTGAA GCTTTTCAGT 
CGCATTTTTA ACTGCAACCC AGATGATATG GCACGGGACC TAGAGCAGGG TGACGTGTCA 
GAGACAATCA GAGTCTTCTT TGAGCAGAGC AAGTCTTTCC CCCCAGCTGC CAAGAGCCTC 
CTTACCATCC AGGAAGTGGA TGAGTTCCTT CTGCGGCTGT CCAAGCTCAC CAAGGAGGAT 
GAGCAGCAAC AGGCCCTACA GGACATTGCC TCCAGGTGTA CAGCCAATGA CCTTAAATGC 
ATCATCAGGT TGATCAAACA TGATCTGAAG ATGAACTCAG GTGCAAAACA TGTGTTAGAC 
GCCCTTGACC CCAATGCCTA TGAAGCCTTC AAAGCCTCGC GCAACCTGCA GGATGTGGTG 
GAGCGGGTCC TTCACAACGC GCAGGAGGTG GAGAAGGAGC CGGGCCAGAG ACGAGCTCTG 
AGCGTCCAGG CCTCGCTGAT GACACCTGTG CAGCCCATGT TGGCGGAGGC CTGCAAGTCC 
GTTGAGTATG CAATGAAGAA ATGTCCCAAT GGCATGTTCT CTGAGATCAA GTACGATGGA 
GAGCGAGTCC AGGTGCATAA GAATGGAGAC CACTTCAGCT ACTTCAGCCG CAGTCTCAAG 
CCCGTCCTTC CTCACAAGGT GGCCCACTTT AAGGACTACA TTCCCCAGGC TTTTCCTGGG 
GGCCACAGCA TGATCTTGGA TTCTGAAGTG CTTCTGATTG ACAACAAGAC AGGCAAACCA 
CTGCCCTTTG GGACTCTGGG AGTACACAAG AAAGCAGCCT TCCAGGATGC TAATGTCTGC 
CTGTTTGTTT TTGATTGTAT CTACTTTAAT GATGTCAGCT TGATGGACAG ACCTCTGTGT 
GAGCGGCGGA AGTTTCTTCA TGACAACATG GTTGAAATTC CAAACCGGAT CATGTTCTCA 
GAAATGAAGC GAGTCACAAA AGCTTTGGAC TTGGCTGACA TGATAACCCG GGTGATCCAG 
GAGGGATTGG AGGGGCTGGT GCTGAAGGAT GTGAAGGGTA CATATGAGCC TGGGAAGCGG 
CACTGGCTGA AAGTGAAGAA AGACTATTTG AACGAGGGGG CCATGGCCGA CACAGCTGAC 
CTGGTGGTCC TTGGAGCCTT CTATGGGCAA GGGAGCAAAG GCGGCATGAT GTCAATCTTC 
CTCATGGGCT GCTACGACCC TGGCAGCCAG AAGTGGTGCA CAGTCACCAA GTGTGCAGGA 
GGCCATGATG ATGCCACGCT TGCCCGCCTG CAGAATGAAC TAGACATGGT GAAGATCAGC 
AAGGACCCCA GCAAAATACC CAGCTGGTTG AAGGTCAACA AGATCTACTA TCCTGACTTC 
ATCGTCCCAG ACCCAAAGAA AGCTGCCGTG TGGGAGATCA CAGGGGCTGA ATTCTCCAAA 
TCGGAGGCTC ATACAGCTGA CGGGATCTCC ATCCGATTCC CTCGCTGCAC CCGAATCCGA 
GATGATAAGG ACTGGAAATC TGCCACTAAC CTTCCCCAAC TCAAGGAACT GTACCAGTTG 
TCCAAGGAGA AGGCAGACTT CACTGTAGTG GCTGGAGATG AGGGGAGCTC CACTACAGGG 
GGTAGCAGTG AAGAGAATAA GGGTCCCTCA GGGTCTGCTG TGTCCCGCAA GGCCCCCAGC 
AAGCCCTCAG CCAGTACCAA GAAAGCAGAA GGGAAGCTGA GTAACTCCAA CAGCAAAGAT 
GGCAACATGC AGACTGCAAA GCCTTCCGCT ATGAAGGTGG GGGAGAAGCT GGCCACAAAG 
TCTTCTCCAG TGAAAGTAGG GGAGAAGCGG AAAGCTGCTG ATGAGACGCT GTGCCAAACA 
AAGAGGCGGC CAGCCAGTGA GCAGAGAGGA AGAACTGTGC CAGCAGGCAG GAGATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( XM_005257970.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258027.1 Homo sapiens DNA ligase 3 (LIG3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu50374
Clone ID Related Accession (Same CDS sequence)	XM_005257970.3 , XM_005257970.4
Accession Version	XM_005257970.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005257970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAAGT TCCGAGGCCT ACGCTATATG TCTTTGGCTT TCAAGATCTT CTTTCCACAA 
ACCCTCCGTG CACTCAGCCG AAAAGAACTG TGCCTATTCC GAAAACATCA CTGGCGTGAT 
GTAAGACAAT TCAGCCAGTG GTCAGAAACA GATCTGCTTC ATGGACATCC CCTCTTCCTG 
AGAAGAAAGC CTGTTCTATC ATTCCAGGGA AGCCATCTAA GATCACGTGC CACCTACCTT 
GTTTTCTTGC CAGGGTTGCA TGTGGGACTC TGCAGTGGCC CCTGTGAGAT GGCTGAGCAA 
CGGTTCTGTG TGGACTATGC CAAGCGTGGC ACAGCTGGCT GCAAAAAATG CAAGGAAAAG 
ATTGTGAAGG GCGTATGCCG AATTGGCAAA GTGGTGCCCA ATCCCTTCTC AGAGTCTGGG 
GGTGATATGA AAGAGTGGTA CCACATTAAA TGCATGTTTG AGAAACTAGA GCGGGCCCGG 
GCCACCACAA AAAAAATCGA GGACCTCACA GAGCTGGAAG GCTGGGAAGA GCTGGAAGAT 
AATGAGAAGG AACAGATAAC CCAGCACATT GCAGATCTGT CTTCTAAGGC AGCAGGTACA 
CCAAAGAAGA AAGCTGTTGT CCAGGCTAAG TTGACAACCA CTGGCCAGGT GACTTCTCCA 
GTGAAAGGCG CCTCATTTGT CACCAGTACC AATCCCCGGA AATTTTCTGG CTTTTCAGCC 
AAGCCCAACA ACTCTGGGGA AGCCCCCTCG AGCCCCACCC CTAAGAGAAG TCTGTCTTCA 
AGCAAATGTG ACCCCAGGCA TAAGGACTGT CTGCTACGGG AGTTTCGAAA GTTATGCGCC 
ATGGTGGCCG ATAATCCTAG CTACAACACG AAGACCCAGA TCATCCAGGA CTTCCTTCGG 
AAAGGCTCAG CAGGAGATGG TTTCCACGGT GATGTGTACC TAACAGTGAA GCTGCTGCTG 
CCAGGAGTCA TTAAGACTGT TTACAACTTG AACGATAAGC AGATTGTGAA GCTTTTCAGT 
CGCATTTTTA ACTGCAACCC AGATGATATG GCACGGGACC TAGAGCAGGG TGACGTGTCA 
GAGACAATCA GAGTCTTCTT TGAGCAGAGC AAGTCTTTCC CCCCAGCTGC CAAGAGCCTC 
CTTACCATCC AGGAAGTGGA TGAGTTCCTT CTGCGGCTGT CCAAGCTCAC CAAGGAGGAT 
GAGCAGCAAC AGGCCCTACA GGACATTGCC TCCAGGTGTA CAGCCAATGA CCTTAAATGC 
ATCATCAGGT TGATCAAACA TGATCTGAAG ATGAACTCAG GTGCAAAACA TGTGTTAGAC 
GCCCTTGACC CCAATGCCTA TGAAGCCTTC AAAGCCTCGC GCAACCTGCA GGATGTGGTG 
GAGCGGGTCC TTCACAACGC GCAGGAGGTG GAGAAGGAGC CGGGCCAGAG ACGAGCTCTG 
AGCGTCCAGG CCTCGCTGAT GACACCTGTG CAGCCCATGT TGGCGGAGGC CTGCAAGTCC 
GTTGAGTATG CAATGAAGAA ATGTCCCAAT GGCATGTTCT CTGAGATCAA GTACGATGGA 
GAGCGAGTCC AGGTGCATAA GAATGGAGAC CACTTCAGCT ACTTCAGCCG CAGTCTCAAG 
CCCGTCCTTC CTCACAAGGT GGCCCACTTT AAGGACTACA TTCCCCAGGC TTTTCCTGGG 
GGCCACAGCA TGATCTTGGA TTCTGAAGTG CTTCTGATTG ACAACAAGAC AGGCAAACCA 
CTGCCCTTTG GGACTCTGGG AGTACACAAG AAAGCAGCCT TCCAGGATGC TAATGTCTGC 
CTGTTTGTTT TTGATTGTAT CTACTTTAAT GATGTCAGCT TGATGGACAG ACCTCTGTGT 
GAGCGGCGGA AGTTTCTTCA TGACAACATG GTTGAAATTC CAAACCGGAT CATGTTCTCA 
GAAATGAAGC GAGTCACAAA AGCTTTGGAC TTGGCTGACA TGATAACCCG GGTGATCCAG 
GAGGGATTGG AGGGGCTGGT GCTGAAGGAT GTGAAGGGTA CATATGAGCC TGGGAAGCGG 
CACTGGCTGA AAGTGAAGAA AGACTATTTG AACGAGGGGG CCATGGCCGA CACAGCTGAC 
CTGGTGGTCC TTGGAGCCTT CTATGGGCAA GGGAGCAAAG GCGGCATGAT GTCAATCTTC 
CTCATGGGCT GCTACGACCC TGGCAGCCAG AAGTGGTGCA CAGTCACCAA GTGTGCAGGA 
GGCCATGATG ATGCCACGCT TGCCCGCCTG CAGAATGAAC TAGACATGGT GAAGATCAGC 
AAGGACCCCA GCAAAATACC CAGCTGGTTG AAGGTCAACA AGATCTACTA TCCTGACTTC 
ATCGTCCCAG ACCCAAAGAA AGCTGCCGTG TGGGAGATCA CAGGGGCTGA ATTCTCCAAA 
TCGGAGGCTC ATACAGCTGA CGGGATCTCC ATCCGATTCC CTCGCTGCAC CCGAATCCGA 
GATGATAAGG ACTGGAAATC TGCCACTAAC CTTCCCCAAC TCAAGGAACT GTACCAGTTG 
TCCAAGGAGA AGGCAGACTT CACTGTAGTG GCTGGAGATG AGGGGAGCTC CACTACAGGG 
GGTAGCAGTG AAGAGAATAA GGGTCCCTCA GGGTCTGCTG TGTCCCGCAA GGCCCCCAGC 
AAGCCCTCAG CCAGTACCAA GAAAGCAGAA GGGAAGCTGA GTAACTCCAA CAGCAAAGAT 
GGCAACATGC AGACTGCAAA GCCTTCCGCT ATGAAGGTGG GGGAGAAGCT GGCCACAAAG 
TCTTCTCCAG TGAAAGTAGG GGAGAAGCGG AAAGCTGCTG ATGAGACGCT GTGCCAAACA 
AAGGTATTGC TGGACATCTT CACTGGGGTG CGGCTTTACT TGCCACCCTC CACACCAGAC 
TTCAGCCGTC TCAGACGCTA CTTTGTGGCA TTCGACGGGG ACCTGGTACA GGAATTTGAT 
ATGACTTCAG CCACGCACGT GCTGGGTAGC AGGGACAAGA ACCCTGCGGC CCAGCAGGTC 
TCCCCAGAGT GGATTTGGGC ATGTATCCGG AAACGGAGAC TGGTAGCTCC CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258027.1
CDS	71..3127
Translation	MSKFRGLRYMSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_005257970.3
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA ligase 3 (LIG3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257970.3

ORF Insert Sequence:

ATGAGCAAGT TCCGAGGCCT ACGCTATATG TCTTTGGCTT TCAAGATCTT CTTTCCACAA 
ACCCTCCGTG CACTCAGCCG AAAAGAACTG TGCCTATTCC GAAAACATCA CTGGCGTGAT 
GTAAGACAAT TCAGCCAGTG GTCAGAAACA GATCTGCTTC ATGGACATCC CCTCTTCCTG 
AGAAGAAAGC CTGTTCTATC ATTCCAGGGA AGCCATCTAA GATCACGTGC CACCTACCTT 
GTTTTCTTGC CAGGGTTGCA TGTGGGACTC TGCAGTGGCC CCTGTGAGAT GGCTGAGCAA 
CGGTTCTGTG TGGACTATGC CAAGCGTGGC ACAGCTGGCT GCAAAAAATG CAAGGAAAAG 
ATTGTGAAGG GCGTATGCCG AATTGGCAAA GTGGTGCCCA ATCCCTTCTC AGAGTCTGGG 
GGTGATATGA AAGAGTGGTA CCACATTAAA TGCATGTTTG AGAAACTAGA GCGGGCCCGG 
GCCACCACAA AAAAAATCGA GGACCTCACA GAGCTGGAAG GCTGGGAAGA GCTGGAAGAT 
AATGAGAAGG AACAGATAAC CCAGCACATT GCAGATCTGT CTTCTAAGGC AGCAGGTACA 
CCAAAGAAGA AAGCTGTTGT CCAGGCTAAG TTGACAACCA CTGGCCAGGT GACTTCTCCA 
GTGAAAGGCG CCTCATTTGT CACCAGTACC AATCCCCGGA AATTTTCTGG CTTTTCAGCC 
AAGCCCAACA ACTCTGGGGA AGCCCCCTCG AGCCCCACCC CTAAGAGAAG TCTGTCTTCA 
AGCAAATGTG ACCCCAGGCA TAAGGACTGT CTGCTACGGG AGTTTCGAAA GTTATGCGCC 
ATGGTGGCCG ATAATCCTAG CTACAACACG AAGACCCAGA TCATCCAGGA CTTCCTTCGG 
AAAGGCTCAG CAGGAGATGG TTTCCACGGT GATGTGTACC TAACAGTGAA GCTGCTGCTG 
CCAGGAGTCA TTAAGACTGT TTACAACTTG AACGATAAGC AGATTGTGAA GCTTTTCAGT 
CGCATTTTTA ACTGCAACCC AGATGATATG GCACGGGACC TAGAGCAGGG TGACGTGTCA 
GAGACAATCA GAGTCTTCTT TGAGCAGAGC AAGTCTTTCC CCCCAGCTGC CAAGAGCCTC 
CTTACCATCC AGGAAGTGGA TGAGTTCCTT CTGCGGCTGT CCAAGCTCAC CAAGGAGGAT 
GAGCAGCAAC AGGCCCTACA GGACATTGCC TCCAGGTGTA CAGCCAATGA CCTTAAATGC 
ATCATCAGGT TGATCAAACA TGATCTGAAG ATGAACTCAG GTGCAAAACA TGTGTTAGAC 
GCCCTTGACC CCAATGCCTA TGAAGCCTTC AAAGCCTCGC GCAACCTGCA GGATGTGGTG 
GAGCGGGTCC TTCACAACGC GCAGGAGGTG GAGAAGGAGC CGGGCCAGAG ACGAGCTCTG 
AGCGTCCAGG CCTCGCTGAT GACACCTGTG CAGCCCATGT TGGCGGAGGC CTGCAAGTCC 
GTTGAGTATG CAATGAAGAA ATGTCCCAAT GGCATGTTCT CTGAGATCAA GTACGATGGA 
GAGCGAGTCC AGGTGCATAA GAATGGAGAC CACTTCAGCT ACTTCAGCCG CAGTCTCAAG 
CCCGTCCTTC CTCACAAGGT GGCCCACTTT AAGGACTACA TTCCCCAGGC TTTTCCTGGG 
GGCCACAGCA TGATCTTGGA TTCTGAAGTG CTTCTGATTG ACAACAAGAC AGGCAAACCA 
CTGCCCTTTG GGACTCTGGG AGTACACAAG AAAGCAGCCT TCCAGGATGC TAATGTCTGC 
CTGTTTGTTT TTGATTGTAT CTACTTTAAT GATGTCAGCT TGATGGACAG ACCTCTGTGT 
GAGCGGCGGA AGTTTCTTCA TGACAACATG GTTGAAATTC CAAACCGGAT CATGTTCTCA 
GAAATGAAGC GAGTCACAAA AGCTTTGGAC TTGGCTGACA TGATAACCCG GGTGATCCAG 
GAGGGATTGG AGGGGCTGGT GCTGAAGGAT GTGAAGGGTA CATATGAGCC TGGGAAGCGG 
CACTGGCTGA AAGTGAAGAA AGACTATTTG AACGAGGGGG CCATGGCCGA CACAGCTGAC 
CTGGTGGTCC TTGGAGCCTT CTATGGGCAA GGGAGCAAAG GCGGCATGAT GTCAATCTTC 
CTCATGGGCT GCTACGACCC TGGCAGCCAG AAGTGGTGCA CAGTCACCAA GTGTGCAGGA 
GGCCATGATG ATGCCACGCT TGCCCGCCTG CAGAATGAAC TAGACATGGT GAAGATCAGC 
AAGGACCCCA GCAAAATACC CAGCTGGTTG AAGGTCAACA AGATCTACTA TCCTGACTTC 
ATCGTCCCAG ACCCAAAGAA AGCTGCCGTG TGGGAGATCA CAGGGGCTGA ATTCTCCAAA 
TCGGAGGCTC ATACAGCTGA CGGGATCTCC ATCCGATTCC CTCGCTGCAC CCGAATCCGA 
GATGATAAGG ACTGGAAATC TGCCACTAAC CTTCCCCAAC TCAAGGAACT GTACCAGTTG 
TCCAAGGAGA AGGCAGACTT CACTGTAGTG GCTGGAGATG AGGGGAGCTC CACTACAGGG 
GGTAGCAGTG AAGAGAATAA GGGTCCCTCA GGGTCTGCTG TGTCCCGCAA GGCCCCCAGC 
AAGCCCTCAG CCAGTACCAA GAAAGCAGAA GGGAAGCTGA GTAACTCCAA CAGCAAAGAT 
GGCAACATGC AGACTGCAAA GCCTTCCGCT ATGAAGGTGG GGGAGAAGCT GGCCACAAAG 
TCTTCTCCAG TGAAAGTAGG GGAGAAGCGG AAAGCTGCTG ATGAGACGCT GTGCCAAACA 
AAGGTATTGC TGGACATCTT CACTGGGGTG CGGCTTTACT TGCCACCCTC CACACCAGAC 
TTCAGCCGTC TCAGACGCTA CTTTGTGGCA TTCGACGGGG ACCTGGTACA GGAATTTGAT 
ATGACTTCAG CCACGCACGT GCTGGGTAGC AGGGACAAGA ACCCTGCGGC CCAGCAGGTC 
TCCCCAGAGT GGATTTGGGC ATGTATCCGG AAACGGAGAC TGGTAGCTCC CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LIG3 ( XM_005257970.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005258027.1 Homo sapiens DNA ligase 3 (LIG3), transcript variant X1, mRNA.

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CloneID	OHu50374
Clone ID Related Accession (Same CDS sequence)	XM_005257970.3 , XM_005257970.4
Accession Version	XM_005257970.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3057bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	DNA ligase 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000017.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005257970.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCAAGT TCCGAGGCCT ACGCTATATG TCTTTGGCTT TCAAGATCTT CTTTCCACAA 
ACCCTCCGTG CACTCAGCCG AAAAGAACTG TGCCTATTCC GAAAACATCA CTGGCGTGAT 
GTAAGACAAT TCAGCCAGTG GTCAGAAACA GATCTGCTTC ATGGACATCC CCTCTTCCTG 
AGAAGAAAGC CTGTTCTATC ATTCCAGGGA AGCCATCTAA GATCACGTGC CACCTACCTT 
GTTTTCTTGC CAGGGTTGCA TGTGGGACTC TGCAGTGGCC CCTGTGAGAT GGCTGAGCAA 
CGGTTCTGTG TGGACTATGC CAAGCGTGGC ACAGCTGGCT GCAAAAAATG CAAGGAAAAG 
ATTGTGAAGG GCGTATGCCG AATTGGCAAA GTGGTGCCCA ATCCCTTCTC AGAGTCTGGG 
GGTGATATGA AAGAGTGGTA CCACATTAAA TGCATGTTTG AGAAACTAGA GCGGGCCCGG 
GCCACCACAA AAAAAATCGA GGACCTCACA GAGCTGGAAG GCTGGGAAGA GCTGGAAGAT 
AATGAGAAGG AACAGATAAC CCAGCACATT GCAGATCTGT CTTCTAAGGC AGCAGGTACA 
CCAAAGAAGA AAGCTGTTGT CCAGGCTAAG TTGACAACCA CTGGCCAGGT GACTTCTCCA 
GTGAAAGGCG CCTCATTTGT CACCAGTACC AATCCCCGGA AATTTTCTGG CTTTTCAGCC 
AAGCCCAACA ACTCTGGGGA AGCCCCCTCG AGCCCCACCC CTAAGAGAAG TCTGTCTTCA 
AGCAAATGTG ACCCCAGGCA TAAGGACTGT CTGCTACGGG AGTTTCGAAA GTTATGCGCC 
ATGGTGGCCG ATAATCCTAG CTACAACACG AAGACCCAGA TCATCCAGGA CTTCCTTCGG 
AAAGGCTCAG CAGGAGATGG TTTCCACGGT GATGTGTACC TAACAGTGAA GCTGCTGCTG 
CCAGGAGTCA TTAAGACTGT TTACAACTTG AACGATAAGC AGATTGTGAA GCTTTTCAGT 
CGCATTTTTA ACTGCAACCC AGATGATATG GCACGGGACC TAGAGCAGGG TGACGTGTCA 
GAGACAATCA GAGTCTTCTT TGAGCAGAGC AAGTCTTTCC CCCCAGCTGC CAAGAGCCTC 
CTTACCATCC AGGAAGTGGA TGAGTTCCTT CTGCGGCTGT CCAAGCTCAC CAAGGAGGAT 
GAGCAGCAAC AGGCCCTACA GGACATTGCC TCCAGGTGTA CAGCCAATGA CCTTAAATGC 
ATCATCAGGT TGATCAAACA TGATCTGAAG ATGAACTCAG GTGCAAAACA TGTGTTAGAC 
GCCCTTGACC CCAATGCCTA TGAAGCCTTC AAAGCCTCGC GCAACCTGCA GGATGTGGTG 
GAGCGGGTCC TTCACAACGC GCAGGAGGTG GAGAAGGAGC CGGGCCAGAG ACGAGCTCTG 
AGCGTCCAGG CCTCGCTGAT GACACCTGTG CAGCCCATGT TGGCGGAGGC CTGCAAGTCC 
GTTGAGTATG CAATGAAGAA ATGTCCCAAT GGCATGTTCT CTGAGATCAA GTACGATGGA 
GAGCGAGTCC AGGTGCATAA GAATGGAGAC CACTTCAGCT ACTTCAGCCG CAGTCTCAAG 
CCCGTCCTTC CTCACAAGGT GGCCCACTTT AAGGACTACA TTCCCCAGGC TTTTCCTGGG 
GGCCACAGCA TGATCTTGGA TTCTGAAGTG CTTCTGATTG ACAACAAGAC AGGCAAACCA 
CTGCCCTTTG GGACTCTGGG AGTACACAAG AAAGCAGCCT TCCAGGATGC TAATGTCTGC 
CTGTTTGTTT TTGATTGTAT CTACTTTAAT GATGTCAGCT TGATGGACAG ACCTCTGTGT 
GAGCGGCGGA AGTTTCTTCA TGACAACATG GTTGAAATTC CAAACCGGAT CATGTTCTCA 
GAAATGAAGC GAGTCACAAA AGCTTTGGAC TTGGCTGACA TGATAACCCG GGTGATCCAG 
GAGGGATTGG AGGGGCTGGT GCTGAAGGAT GTGAAGGGTA CATATGAGCC TGGGAAGCGG 
CACTGGCTGA AAGTGAAGAA AGACTATTTG AACGAGGGGG CCATGGCCGA CACAGCTGAC 
CTGGTGGTCC TTGGAGCCTT CTATGGGCAA GGGAGCAAAG GCGGCATGAT GTCAATCTTC 
CTCATGGGCT GCTACGACCC TGGCAGCCAG AAGTGGTGCA CAGTCACCAA GTGTGCAGGA 
GGCCATGATG ATGCCACGCT TGCCCGCCTG CAGAATGAAC TAGACATGGT GAAGATCAGC 
AAGGACCCCA GCAAAATACC CAGCTGGTTG AAGGTCAACA AGATCTACTA TCCTGACTTC 
ATCGTCCCAG ACCCAAAGAA AGCTGCCGTG TGGGAGATCA CAGGGGCTGA ATTCTCCAAA 
TCGGAGGCTC ATACAGCTGA CGGGATCTCC ATCCGATTCC CTCGCTGCAC CCGAATCCGA 
GATGATAAGG ACTGGAAATC TGCCACTAAC CTTCCCCAAC TCAAGGAACT GTACCAGTTG 
TCCAAGGAGA AGGCAGACTT CACTGTAGTG GCTGGAGATG AGGGGAGCTC CACTACAGGG 
GGTAGCAGTG AAGAGAATAA GGGTCCCTCA GGGTCTGCTG TGTCCCGCAA GGCCCCCAGC 
AAGCCCTCAG CCAGTACCAA GAAAGCAGAA GGGAAGCTGA GTAACTCCAA CAGCAAAGAT 
GGCAACATGC AGACTGCAAA GCCTTCCGCT ATGAAGGTGG GGGAGAAGCT GGCCACAAAG 
TCTTCTCCAG TGAAAGTAGG GGAGAAGCGG AAAGCTGCTG ATGAGACGCT GTGCCAAACA 
AAGGTATTGC TGGACATCTT CACTGGGGTG CGGCTTTACT TGCCACCCTC CACACCAGAC 
TTCAGCCGTC TCAGACGCTA CTTTGTGGCA TTCGACGGGG ACCTGGTACA GGAATTTGAT 
ATGACTTCAG CCACGCACGT GCTGGGTAGC AGGGACAAGA ACCCTGCGGC CCAGCAGGTC 
TCCCCAGAGT GGATTTGGGC ATGTATCCGG AAACGGAGAC TGGTAGCTCC CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005258027.1
CDS	72..3128
Translation	MSKFRGLRYMSLAFKIFFPQTLRALSRKELCLFRKHHWRDVRQFSQWSETDLLHGHPLFLRRKPVLSFQGSHLRSRATYLVFLPGLHVGLCSGPCEMAEQRFCVDYAKRGTAGCKKCKEKIVKGVCRIGKVVPNPFSESGGDMKEWYHIKCMFEKLERARATTKKIEDLTELEGWEELEDNEKEQITQHIADLSSKAAGTPKKKAVVQAKLTTTGQVTSPVKGASFVTSTNPRKFSGFSAKPNNSGEAPSSPTPKRSLSSSKCDPRHKDCLLREFRKLCAMVADNPSYNTKTQIIQDFLRKGSAGDGFHGDVYLTVKLLLPGVIKTVYNLNDKQIVKLFSRIFNCNPDDMARDLEQGDVSETIRVFFEQSKSFPPAAKSLLTIQEVDEFLLRLSKLTKEDEQQQALQDIASRCTANDLKCIIRLIKHDLKMNSGAKHVLDALDPNAYEAFKASRNLQDVVERVLHNAQEVEKEPGQRRALSVQASLMTPVQPMLAEACKSVEYAMKKCPNGMFSEIKYDGERVQVHKNGDHFSYFSRSLKPVLPHKVAHFKDYIPQAFPGGHSMILDSEVLLIDNKTGKPLPFGTLGVHKKAAFQDANVCLFVFDCIYFNDVSLMDRPLCERRKFLHDNMVEIPNRIMFSEMKRVTKALDLADMITRVIQEGLEGLVLKDVKGTYEPGKRHWLKVKKDYLNEGAMADTADLVVLGAFYGQGSKGGMMSIFLMGCYDPGSQKWCTVTKCAGGHDDATLARLQNELDMVKISKDPSKIPSWLKVNKIYYPDFIVPDPKKAAVWEITGAEFSKSEAHTADGISIRFPRCTRIRDDKDWKSATNLPQLKELYQLSKEKADFTVVAGDEGSSTTGGSSEENKGPSGSAVSRKAPSKPSASTKKAEGKLSNSNSKDGNMQTAKPSAMKVGEKLATKSSPVKVGEKRKAADETLCQTKVLLDIFTGVRLYLPPSTPDFSRLRRYFVAFDGDLVQEFDMTSATHVLGSRDKNPAAQQVSPEWIWACIRKRRLVAPC

Target ORF information:

RefSeq Version	XM_005257970.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA ligase 3 (LIG3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005257970.4

ORF Insert Sequence:

ATGAGCAAGT TCCGAGGCCT ACGCTATATG TCTTTGGCTT TCAAGATCTT CTTTCCACAA 
ACCCTCCGTG CACTCAGCCG AAAAGAACTG TGCCTATTCC GAAAACATCA CTGGCGTGAT 
GTAAGACAAT TCAGCCAGTG GTCAGAAACA GATCTGCTTC ATGGACATCC CCTCTTCCTG 
AGAAGAAAGC CTGTTCTATC ATTCCAGGGA AGCCATCTAA GATCACGTGC CACCTACCTT 
GTTTTCTTGC CAGGGTTGCA TGTGGGACTC TGCAGTGGCC CCTGTGAGAT GGCTGAGCAA 
CGGTTCTGTG TGGACTATGC CAAGCGTGGC ACAGCTGGCT GCAAAAAATG CAAGGAAAAG 
ATTGTGAAGG GCGTATGCCG AATTGGCAAA GTGGTGCCCA ATCCCTTCTC AGAGTCTGGG 
GGTGATATGA AAGAGTGGTA CCACATTAAA TGCATGTTTG AGAAACTAGA GCGGGCCCGG 
GCCACCACAA AAAAAATCGA GGACCTCACA GAGCTGGAAG GCTGGGAAGA GCTGGAAGAT 
AATGAGAAGG AACAGATAAC CCAGCACATT GCAGATCTGT CTTCTAAGGC AGCAGGTACA 
CCAAAGAAGA AAGCTGTTGT CCAGGCTAAG TTGACAACCA CTGGCCAGGT GACTTCTCCA 
GTGAAAGGCG CCTCATTTGT CACCAGTACC AATCCCCGGA AATTTTCTGG CTTTTCAGCC 
AAGCCCAACA ACTCTGGGGA AGCCCCCTCG AGCCCCACCC CTAAGAGAAG TCTGTCTTCA 
AGCAAATGTG ACCCCAGGCA TAAGGACTGT CTGCTACGGG AGTTTCGAAA GTTATGCGCC 
ATGGTGGCCG ATAATCCTAG CTACAACACG AAGACCCAGA TCATCCAGGA CTTCCTTCGG 
AAAGGCTCAG CAGGAGATGG TTTCCACGGT GATGTGTACC TAACAGTGAA GCTGCTGCTG 
CCAGGAGTCA TTAAGACTGT TTACAACTTG AACGATAAGC AGATTGTGAA GCTTTTCAGT 
CGCATTTTTA ACTGCAACCC AGATGATATG GCACGGGACC TAGAGCAGGG TGACGTGTCA 
GAGACAATCA GAGTCTTCTT TGAGCAGAGC AAGTCTTTCC CCCCAGCTGC CAAGAGCCTC 
CTTACCATCC AGGAAGTGGA TGAGTTCCTT CTGCGGCTGT CCAAGCTCAC CAAGGAGGAT 
GAGCAGCAAC AGGCCCTACA GGACATTGCC TCCAGGTGTA CAGCCAATGA CCTTAAATGC 
ATCATCAGGT TGATCAAACA TGATCTGAAG ATGAACTCAG GTGCAAAACA TGTGTTAGAC 
GCCCTTGACC CCAATGCCTA TGAAGCCTTC AAAGCCTCGC GCAACCTGCA GGATGTGGTG 
GAGCGGGTCC TTCACAACGC GCAGGAGGTG GAGAAGGAGC CGGGCCAGAG ACGAGCTCTG 
AGCGTCCAGG CCTCGCTGAT GACACCTGTG CAGCCCATGT TGGCGGAGGC CTGCAAGTCC 
GTTGAGTATG CAATGAAGAA ATGTCCCAAT GGCATGTTCT CTGAGATCAA GTACGATGGA 
GAGCGAGTCC AGGTGCATAA GAATGGAGAC CACTTCAGCT ACTTCAGCCG CAGTCTCAAG 
CCCGTCCTTC CTCACAAGGT GGCCCACTTT AAGGACTACA TTCCCCAGGC TTTTCCTGGG 
GGCCACAGCA TGATCTTGGA TTCTGAAGTG CTTCTGATTG ACAACAAGAC AGGCAAACCA 
CTGCCCTTTG GGACTCTGGG AGTACACAAG AAAGCAGCCT TCCAGGATGC TAATGTCTGC 
CTGTTTGTTT TTGATTGTAT CTACTTTAAT GATGTCAGCT TGATGGACAG ACCTCTGTGT 
GAGCGGCGGA AGTTTCTTCA TGACAACATG GTTGAAATTC CAAACCGGAT CATGTTCTCA 
GAAATGAAGC GAGTCACAAA AGCTTTGGAC TTGGCTGACA TGATAACCCG GGTGATCCAG 
GAGGGATTGG AGGGGCTGGT GCTGAAGGAT GTGAAGGGTA CATATGAGCC TGGGAAGCGG 
CACTGGCTGA AAGTGAAGAA AGACTATTTG AACGAGGGGG CCATGGCCGA CACAGCTGAC 
CTGGTGGTCC TTGGAGCCTT CTATGGGCAA GGGAGCAAAG GCGGCATGAT GTCAATCTTC 
CTCATGGGCT GCTACGACCC TGGCAGCCAG AAGTGGTGCA CAGTCACCAA GTGTGCAGGA 
GGCCATGATG ATGCCACGCT TGCCCGCCTG CAGAATGAAC TAGACATGGT GAAGATCAGC 
AAGGACCCCA GCAAAATACC CAGCTGGTTG AAGGTCAACA AGATCTACTA TCCTGACTTC 
ATCGTCCCAG ACCCAAAGAA AGCTGCCGTG TGGGAGATCA CAGGGGCTGA ATTCTCCAAA 
TCGGAGGCTC ATACAGCTGA CGGGATCTCC ATCCGATTCC CTCGCTGCAC CCGAATCCGA 
GATGATAAGG ACTGGAAATC TGCCACTAAC CTTCCCCAAC TCAAGGAACT GTACCAGTTG 
TCCAAGGAGA AGGCAGACTT CACTGTAGTG GCTGGAGATG AGGGGAGCTC CACTACAGGG 
GGTAGCAGTG AAGAGAATAA GGGTCCCTCA GGGTCTGCTG TGTCCCGCAA GGCCCCCAGC 
AAGCCCTCAG CCAGTACCAA GAAAGCAGAA GGGAAGCTGA GTAACTCCAA CAGCAAAGAT 
GGCAACATGC AGACTGCAAA GCCTTCCGCT ATGAAGGTGG GGGAGAAGCT GGCCACAAAG 
TCTTCTCCAG TGAAAGTAGG GGAGAAGCGG AAAGCTGCTG ATGAGACGCT GTGCCAAACA 
AAGGTATTGC TGGACATCTT CACTGGGGTG CGGCTTTACT TGCCACCCTC CACACCAGAC 
TTCAGCCGTC TCAGACGCTA CTTTGTGGCA TTCGACGGGG ACCTGGTACA GGAATTTGAT 
ATGACTTCAG CCACGCACGT GCTGGGTAGC AGGGACAAGA ACCCTGCGGC CCAGCAGGTC 
TCCCCAGAGT GGATTTGGGC ATGTATCCGG AAACGGAGAC TGGTAGCTCC CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Kinetic analyses of single-stranded break repair by human DNA ligase III isoforms reveal biochemical differences from DNA ligase I.
The Journal of biological chemistry292(38)15870-15879(2017 09)
McNally JR,O'Brien PJ

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LIG3 cDNA ORF clone, Homo sapiens(human)

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gRNAs

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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