MAP3K15 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAP3K15
Entrez Gene ID	389840
Full Name	mitogen-activated protein kinase kinase kinase 15
Synonyms	ASK3,bA723P2.3
General protein information	Preferred Names mitogen-activated protein kinase kinase kinase 15 Names mitogen-activated protein kinase kinase kinase 15 MAPK/ERK kinase kinase 15 MEK kinase 15 MEKK 15 apoptosis signal-regulating kinase 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.12
Summary	The protein encoded by this gene is a member of the mitogen-activated protein kinase (MAPK) family. These family members function in a protein kinase signal transduction cascade, where an activated MAPK kinase kinase (MAP3K) phosphorylates and activates a specific MAPK kinase (MAP2K), which then activates a specific MAPK. This MAP3K protein plays an essential role in apoptotic cell death triggered by cellular stresses. [provided by RefSeq, Jul 2010].
Disorder MIM:	300820

mRNA and Protein(s)

mRNA	Protein	Name
XM_011545508.2	XP_011543810.2	mitogen-activated protein kinase kinase kinase 15 isoform X2
XM_011545508.3	XP_011543810.3	mitogen-activated protein kinase kinase kinase 15 isoform X2
XM_017029511.1	XP_016885000.1	mitogen-activated protein kinase kinase kinase 15 isoform X4
NM_001001671.3	NP_001001671.3	mitogen-activated protein kinase kinase kinase 15
XM_011545507.2	XP_011543809.2	mitogen-activated protein kinase kinase kinase 15 isoform X1
XM_011545507.3	XP_011543809.3	mitogen-activated protein kinase kinase kinase 15 isoform X1
XM_011545510.2	XP_011543812.1	mitogen-activated protein kinase kinase kinase 15 isoform X3
XM_011545511.2	XP_011543813.1	mitogen-activated protein kinase kinase kinase 15 isoform X4

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAP3K15	NP_001001671.3
Bos taurus (cattle)	MAP3K15	XP_002700399.1
Rattus norvegicus (Norway rat)	Map3k15	XP_006256994.1
Gallus gallus (chicken)	MAP3K15	XP_003640537.1
Drosophila melanogaster (fruit fly)	Pk92B	NP_477089.3
	LOC695958	XP_002808565.1
Canis lupus familiaris (dog)	MAP3K15	XP_548885.3
Mus musculus (house mouse)	Map3k15	NP_001156557.2
Danio rerio (zebrafish)	map3k15	XP_003201359.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000747	XP_311281.5

Gene Ontology
Bibliography

Related articles in PubMed

ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney.
Naguro I, Umeda T, Kobayashi Y, Maruyama J, Hattori K, Shimizu Y, Kataoka K, Kim-Mitsuyama S, Uchida S, Vandewalle A, Noguchi T, Nishitoh H, Matsuzawa A, Takeda K, Ichijo H
Nature communications31285(2012)

ASK3, a novel member of the apoptosis signal-regulating kinase family, is essential for stress-induced cell death in HeLa cells.
Kaji T, Yoshida S, Kawai K, Fuchigami Y, Watanabe W, Kubodera H, Kishimoto T
Biochemical and biophysical research communications395(2)213-8(2010 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

"ASK3 gene" is a novel member of apoptosis signal-regulating kinases and that it plays a pivotal role in the signal transduction pathway implicated in apoptotic cell death triggered by cellular stresses.
Title: ASK3, a novel member of the apoptosis signal-regulating kinase family, is essential for stress-induced cell death in HeLa cells.

The following MAP3K15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAP3K15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu80029	XM_011545510.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OHu80030	XM_011545511.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
OHu80026	XM_011545507.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu80027	XM_011545508.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu21561	NM_001001671.3 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu99304	XM_011545507.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu99305	XM_011545508.2 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu99306	XM_017029511.1 Latest version!	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAP3K15 ( XM_011545510.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543812.1 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu80029
Clone ID Related Accession (Same CDS sequence)	XM_011545510.2
Accession Version	XM_011545510.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545510.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACAATT ACTGGGATGT GGGTCAGTTC TTCAGCGTCA GCATGCTGGC CCATGATGTC 
GGGAAAGCCG TCCAGGCAGC AGAGAGGTTG TTCAAACTGA AACCTCCAGT CTGGTACCTG 
CGATCATTAG TTCAGAACTT GTTACTAATT CGGCGCTTCA AGAAAACCAT TATTGAACAC 
TCGCCCAGGC AAGAGCGGCT GAACTTCTGG TTAGATATAA TTTTTGAGGC AACAAATGAA 
GTCACTAATG GACTCAGATT TCCAGTTCTG GTCATAGAGC CAACCAAAGT GTACCAGCCT 
TCTTATGTTT CCATAAACAA TGAAGCCGAG GAGAGAACAG TTTCTTTATG GCATGTCTCA 
CCCACAGAAA TGAAACAGAT GCACGAATGG AATTTTACAG CCTCTTCCAT AAAGGGAATA 
AGCCTATCAA AGTTTGATGA AAGGTGTTGT TTTCTTTATG TCCATGATAA TTCTGATGAC 
TTTCAAATCT ACTTTTCCAC CGAAGAGCAG TGCAGTAGAT TTTTCTCTTT GGTCAAAGAG 
ATGATAACCA ATACAGCAGG CAGTACGGTG GAGCTGGAGG GAGAGACCGA TGGAGACACC 
TTGGAGTATG AGTATGACCA TGATGCAAAT GGTGAGAGAG TTGTCTTGGG GAAAGGCACG 
TATGGGATTG TGTATGCTGG CCGAGATCTG AGCAATCAAG TGCGAATAGC CATCAAAGAA 
ATCCCGGAGA GAGATAGCAG GTATTCTCAG CCTCTGCACG AGGAGATAGC CCTGCACAAG 
TACCTTAAGC ACCGCAATAT CGTTCAGTAC CTGGGCTCTG TTTCAGAGAA CGGCTACATT 
AAGATATTTA TGGAGCAGGT GCCTGGAGGA AGCCTTTCTG CTCTTCTGCG ATCCAAATGG 
GGGCCGATGA AGGAACCGAC AATCAAGTTT TACACCAAAC AGATCCTGGA GGGCCTTAAG 
TATCTTCATG AAAACCAGAT CGTGCACAGA GACATAAAGG GCGATAATGT TCTGGTGAAC 
ACCTACAGCG GAGTGGTGAA AATCTCCGAT TTTGGAACCT CGAAACGTCT TGCGGGTGTG 
AACCCCTGCA CAGAGACTTT TACTGGCACC CTGCAGTACA TGGCACCTGA GATAATTGAC 
CAAGGGCCTC GCGGATATGG TGCCCCAGCC GATATCTGGT CCCTGGGCTG CACCATCATT 
GAGATGGCCA CCAGCAAGCC TCCGTTCCAT GAGCTTGGTG AGCCGCAGGC AGCCATGTTC 
AAAGTGGGCA TGTTTAAGAT CCACCCTGAG ATTCCAGAAG CCCTTTCAGC TGAAGCCCGA 
GCCTTCATTT TATCCTGTTT CGAGCCTGAC CCCCACAAAC GTGCCACCAC TGCTGAGCTA 
CTGAGAGAGG GTTTCTTAAG GCAGGTGAAC AAGGGCAAGA AGAACCGAAT TGCCTTCAAG 
CCCTCAGAAG GTCCCCGCGG TGTCGTCCTG GCCCTGCCCA CACAGGGAGA GCCCATGGCC 
ACCAGCAGCA GCGAGCACGG CTCTGTCTCC CCAGACTCCG ACGCCCAGCC TGACGCACTC 
TTTGAGAGGA CCCGGGCGCC CAGGCACCAC CTTGGCCACC TCCTCAGTGT TCCAGACGAG 
AGCTCAGCCT TGGAAGACCG GGGCTTGGCC TCGTCCCCGG AGGACAGGGA CCAGGGCCTC 
TTCCTGCTAC GCAAGGACAG TGAGCGCCGT GCCATCCTGT ACAAAATCCT CTGGGAGGAG 
CAGAACCAGG TGGCTTCCAA CCTGCAGGAG TGTGTGGCCC AGAGTTCCGA AGAGTTGCAT 
CTCTCAGTTG GACACATCAA GCAAATCATT GGGATCCTGA GGGACTTCAT CCGCTCCCCA 
GAGCACCGGG TGATGGCGAC CACAATATCA AAGCTCAAGG TGGACCTGGA CTTTGACAGC 
TCGTCCATCA GTCAGATTCA CCTGGTGCTG TTCGGATTTC AGGATGCCGT AAATAAAATT 
TTGAGGAACC ACTTAATTAG GCCCCACTGG ATGTTCGCGA TGGACAACAT CATCCGCCGA 
GCGGTGCAGG CCGCGGTCAC CATTCTCATC CCAGAGCTCC GAGCCCACTT TGAGCCTACC 
TGTGAGACTG AAGGGGTAGA TAAGGACATG GATGAAGCGG AAGAGGGCTA TCCCCCAGCC 
ACCGGACCTG GCCAGGAGGC CCAGCCCCAC CAGCAGCACC TGAGCCTCCA GCTGGGTGAG 
CTCAGACAGG AGACCAACAG ACTTTTGGAA CACCTAGTTG AAAAAGAGAG AGAGTACCAG 
AATCTTCTGC GGCAAACTCT AGAACAGAAA ACTCAAGAAT TGTATCACCT TCAGTTAAAA 
TTAAAATCGA ATTGTATTAC AGAGAACCCA GCAGGCCCCT ACGGGCAGAG AACAGATAAA 
GAGCTTATAG ACTGGTTGCG GCTGCAAGGA GCTGATGCAA AGACAATTGA AAAGATTGTT 
GAAGAGGGTT ATACACTTTC GGATATTCTT AATGAGATCA CTAAGGAAGA TCTAAGATAC 
CTTCGACTAC GGGGTGGTCT CCTCTGCAGA CTCTGGAGTG CGGTCTCCCA GTACAGAAGG 
GCTCAGGAGG CCTCAGAAAC CAAAGACAAG GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543812.1
CDS	2067..4682
Translation	MNNYWDVGQFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGSVSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA

Target ORF information:

RefSeq Version	XM_011545510.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545510.2

ORF Insert Sequence:

ATGAACAATT ACTGGGATGT GGGTCAGTTC TTCAGCGTCA GCATGCTGGC CCATGATGTC 
GGGAAAGCCG TCCAGGCAGC AGAGAGGTTG TTCAAACTGA AACCTCCAGT CTGGTACCTG 
CGATCATTAG TTCAGAACTT GTTACTAATT CGGCGCTTCA AGAAAACCAT TATTGAACAC 
TCGCCCAGGC AAGAGCGGCT GAACTTCTGG TTAGATATAA TTTTTGAGGC AACAAATGAA 
GTCACTAATG GACTCAGATT TCCAGTTCTG GTCATAGAGC CAACCAAAGT GTACCAGCCT 
TCTTATGTTT CCATAAACAA TGAAGCCGAG GAGAGAACAG TTTCTTTATG GCATGTCTCA 
CCCACAGAAA TGAAACAGAT GCACGAATGG AATTTTACAG CCTCTTCCAT AAAGGGAATA 
AGCCTATCAA AGTTTGATGA AAGGTGTTGT TTTCTTTATG TCCATGATAA TTCTGATGAC 
TTTCAAATCT ACTTTTCCAC CGAAGAGCAG TGCAGTAGAT TTTTCTCTTT GGTCAAAGAG 
ATGATAACCA ATACAGCAGG CAGTACGGTG GAGCTGGAGG GAGAGACCGA TGGAGACACC 
TTGGAGTATG AGTATGACCA TGATGCAAAT GGTGAGAGAG TTGTCTTGGG GAAAGGCACG 
TATGGGATTG TGTATGCTGG CCGAGATCTG AGCAATCAAG TGCGAATAGC CATCAAAGAA 
ATCCCGGAGA GAGATAGCAG GTATTCTCAG CCTCTGCACG AGGAGATAGC CCTGCACAAG 
TACCTTAAGC ACCGCAATAT CGTTCAGTAC CTGGGCTCTG TTTCAGAGAA CGGCTACATT 
AAGATATTTA TGGAGCAGGT GCCTGGAGGA AGCCTTTCTG CTCTTCTGCG ATCCAAATGG 
GGGCCGATGA AGGAACCGAC AATCAAGTTT TACACCAAAC AGATCCTGGA GGGCCTTAAG 
TATCTTCATG AAAACCAGAT CGTGCACAGA GACATAAAGG GCGATAATGT TCTGGTGAAC 
ACCTACAGCG GAGTGGTGAA AATCTCCGAT TTTGGAACCT CGAAACGTCT TGCGGGTGTG 
AACCCCTGCA CAGAGACTTT TACTGGCACC CTGCAGTACA TGGCACCTGA GATAATTGAC 
CAAGGGCCTC GCGGATATGG TGCCCCAGCC GATATCTGGT CCCTGGGCTG CACCATCATT 
GAGATGGCCA CCAGCAAGCC TCCGTTCCAT GAGCTTGGTG AGCCGCAGGC AGCCATGTTC 
AAAGTGGGCA TGTTTAAGAT CCACCCTGAG ATTCCAGAAG CCCTTTCAGC TGAAGCCCGA 
GCCTTCATTT TATCCTGTTT CGAGCCTGAC CCCCACAAAC GTGCCACCAC TGCTGAGCTA 
CTGAGAGAGG GTTTCTTAAG GCAGGTGAAC AAGGGCAAGA AGAACCGAAT TGCCTTCAAG 
CCCTCAGAAG GTCCCCGCGG TGTCGTCCTG GCCCTGCCCA CACAGGGAGA GCCCATGGCC 
ACCAGCAGCA GCGAGCACGG CTCTGTCTCC CCAGACTCCG ACGCCCAGCC TGACGCACTC 
TTTGAGAGGA CCCGGGCGCC CAGGCACCAC CTTGGCCACC TCCTCAGTGT TCCAGACGAG 
AGCTCAGCCT TGGAAGACCG GGGCTTGGCC TCGTCCCCGG AGGACAGGGA CCAGGGCCTC 
TTCCTGCTAC GCAAGGACAG TGAGCGCCGT GCCATCCTGT ACAAAATCCT CTGGGAGGAG 
CAGAACCAGG TGGCTTCCAA CCTGCAGGAG TGTGTGGCCC AGAGTTCCGA AGAGTTGCAT 
CTCTCAGTTG GACACATCAA GCAAATCATT GGGATCCTGA GGGACTTCAT CCGCTCCCCA 
GAGCACCGGG TGATGGCGAC CACAATATCA AAGCTCAAGG TGGACCTGGA CTTTGACAGC 
TCGTCCATCA GTCAGATTCA CCTGGTGCTG TTCGGATTTC AGGATGCCGT AAATAAAATT 
TTGAGGAACC ACTTAATTAG GCCCCACTGG ATGTTCGCGA TGGACAACAT CATCCGCCGA 
GCGGTGCAGG CCGCGGTCAC CATTCTCATC CCAGAGCTCC GAGCCCACTT TGAGCCTACC 
TGTGAGACTG AAGGGGTAGA TAAGGACATG GATGAAGCGG AAGAGGGCTA TCCCCCAGCC 
ACCGGACCTG GCCAGGAGGC CCAGCCCCAC CAGCAGCACC TGAGCCTCCA GCTGGGTGAG 
CTCAGACAGG AGACCAACAG ACTTTTGGAA CACCTAGTTG AAAAAGAGAG AGAGTACCAG 
AATCTTCTGC GGCAAACTCT AGAACAGAAA ACTCAAGAAT TGTATCACCT TCAGTTAAAA 
TTAAAATCGA ATTGTATTAC AGAGAACCCA GCAGGCCCCT ACGGGCAGAG AACAGATAAA 
GAGCTTATAG ACTGGTTGCG GCTGCAAGGA GCTGATGCAA AGACAATTGA AAAGATTGTT 
GAAGAGGGTT ATACACTTTC GGATATTCTT AATGAGATCA CTAAGGAAGA TCTAAGATAC 
CTTCGACTAC GGGGTGGTCT CCTCTGCAGA CTCTGGAGTG CGGTCTCCCA GTACAGAAGG 
GCTCAGGAGG CCTCAGAAAC CAAAGACAAG GCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K15 ( XM_011545511.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543813.1 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu80030
Clone ID Related Accession (Same CDS sequence)	XM_011545511.2
Accession Version	XM_011545511.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545511.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAACAGA TGCACGAATG GAATTTTACA GCCTCTTCCA TAAAGGGAAT AAGCCTATCA 
AAGTTTGATG AAAGGTGTTG TTTTCTTTAT GTCCATGATA ATTCTGATGA CTTTCAAATC 
TACTTTTCCA CCGAAGAGCA GTGCAGTAGA TTTTTCTCTT TGGTCAAAGA GATGATAACC 
AATACAGCAG GCAGTACGGT GGAGCTGGAG GGAGAGACCG ATGGAGACAC CTTGGAGTAT 
GAGTATGACC ATGATGCAAA TGGTGAGAGA GTTGTCTTGG GGAAAGGCAC GTATGGGATT 
GTGTATGCTG GCCGAGATCT GAGCAATCAA GTGCGAATAG CCATCAAAGA AATCCCGGAG 
AGAGATAGCA GGTATTCTCA GCCTCTGCAC GAGGAGATAG CCCTGCACAA GTACCTTAAG 
CACCGCAATA TCGTTCAGTA CCTGGGCTCT GTTTCAGAGA ACGGCTACAT TAAGATATTT 
ATGGAGCAGG TGCCTGGAGG AAGCCTTTCT GCTCTTCTGC GATCCAAATG GGGGCCGATG 
AAGGAACCGA CAATCAAGTT TTACACCAAA CAGATCCTGG AGGGCCTTAA GTATCTTCAT 
GAAAACCAGA TCGTGCACAG AGACATAAAG GGCGATAATG TTCTGGTGAA CACCTACAGC 
GGAGTGGTGA AAATCTCCGA TTTTGGAACC TCGAAACGTC TTGCGGGTGT GAACCCCTGC 
ACAGAGACTT TTACTGGCAC CCTGCAGTAC ATGGCACCTG AGATAATTGA CCAAGGGCCT 
CGCGGATATG GTGCCCCAGC CGATATCTGG TCCCTGGGCT GCACCATCAT TGAGATGGCC 
ACCAGCAAGC CTCCGTTCCA TGAGCTTGGT GAGCCGCAGG CAGCCATGTT CAAAGTGGGC 
ATGTTTAAGA TCCACCCTGA GATTCCAGAA GCCCTTTCAG CTGAAGCCCG AGCCTTCATT 
TTATCCTGTT TCGAGCCTGA CCCCCACAAA CGTGCCACCA CTGCTGAGCT ACTGAGAGAG 
GGTTTCTTAA GGCAGGTGAA CAAGGGCAAG AAGAACCGAA TTGCCTTCAA GCCCTCAGAA 
GGTCCCCGCG GTGTCGTCCT GGCCCTGCCC ACACAGGGAG AGCCCATGGC CACCAGCAGC 
AGCGAGCACG GCTCTGTCTC CCCAGACTCC GACGCCCAGC CTGACGCACT CTTTGAGAGG 
ACCCGGGCGC CCAGGCACCA CCTTGGCCAC CTCCTCAGTG TTCCAGACGA GAGCTCAGCC 
TTGGAAGACC GGGGCTTGGC CTCGTCCCCG GAGGACAGGG ACCAGGGCCT CTTCCTGCTA 
CGCAAGGACA GTGAGCGCCG TGCCATCCTG TACAAAATCC TCTGGGAGGA GCAGAACCAG 
GTGGCTTCCA ACCTGCAGGA GTGTGTGGCC CAGAGTTCCG AAGAGTTGCA TCTCTCAGTT 
GGACACATCA AGCAAATCAT TGGGATCCTG AGGGACTTCA TCCGCTCCCC AGAGCACCGG 
GTGATGGCGA CCACAATATC AAAGCTCAAG GTGGACCTGG ACTTTGACAG CTCGTCCATC 
AGTCAGATTC ACCTGGTGCT GTTCGGATTT CAGGATGCCG TAAATAAAAT TTTGAGGAAC 
CACTTAATTA GGCCCCACTG GATGTTCGCG ATGGACAACA TCATCCGCCG AGCGGTGCAG 
GCCGCGGTCA CCATTCTCAT CCCAGAGCTC CGAGCCCACT TTGAGCCTAC CTGTGAGACT 
GAAGGGGTAG ATAAGGACAT GGATGAAGCG GAAGAGGGCT ATCCCCCAGC CACCGGACCT 
GGCCAGGAGG CCCAGCCCCA CCAGCAGCAC CTGAGCCTCC AGCTGGGTGA GCTCAGACAG 
GAGACCAACA GACTTTTGGA ACACCTAGTT GAAAAAGAGA GAGAGTACCA GAATCTTCTG 
CGGCAAACTC TAGAACAGAA AACTCAAGAA TTGTATCACC TTCAGTTAAA ATTAAAATCG 
AATTGTATTA CAGAGAACCC AGCAGGCCCC TACGGGCAGA GAACAGATAA AGAGCTTATA 
GACTGGTTGC GGCTGCAAGG AGCTGATGCA AAGACAATTG AAAAGATTGT TGAAGAGGGT 
TATACACTTT CGGATATTCT TAATGAGATC ACTAAGGAAG ATCTAAGATA CCTTCGACTA 
CGGGGTGGTC TCCTCTGCAG ACTCTGGAGT GCGGTCTCCC AGTACAGAAG GGCTCAGGAG 
GCCTCAGAAA CCAAAGACAA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543813.1
CDS	947..3193
Translation	MKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGSVSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA

Target ORF information:

RefSeq Version	XM_011545511.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545511.2

ORF Insert Sequence:

ATGAAACAGA TGCACGAATG GAATTTTACA GCCTCTTCCA TAAAGGGAAT AAGCCTATCA 
AAGTTTGATG AAAGGTGTTG TTTTCTTTAT GTCCATGATA ATTCTGATGA CTTTCAAATC 
TACTTTTCCA CCGAAGAGCA GTGCAGTAGA TTTTTCTCTT TGGTCAAAGA GATGATAACC 
AATACAGCAG GCAGTACGGT GGAGCTGGAG GGAGAGACCG ATGGAGACAC CTTGGAGTAT 
GAGTATGACC ATGATGCAAA TGGTGAGAGA GTTGTCTTGG GGAAAGGCAC GTATGGGATT 
GTGTATGCTG GCCGAGATCT GAGCAATCAA GTGCGAATAG CCATCAAAGA AATCCCGGAG 
AGAGATAGCA GGTATTCTCA GCCTCTGCAC GAGGAGATAG CCCTGCACAA GTACCTTAAG 
CACCGCAATA TCGTTCAGTA CCTGGGCTCT GTTTCAGAGA ACGGCTACAT TAAGATATTT 
ATGGAGCAGG TGCCTGGAGG AAGCCTTTCT GCTCTTCTGC GATCCAAATG GGGGCCGATG 
AAGGAACCGA CAATCAAGTT TTACACCAAA CAGATCCTGG AGGGCCTTAA GTATCTTCAT 
GAAAACCAGA TCGTGCACAG AGACATAAAG GGCGATAATG TTCTGGTGAA CACCTACAGC 
GGAGTGGTGA AAATCTCCGA TTTTGGAACC TCGAAACGTC TTGCGGGTGT GAACCCCTGC 
ACAGAGACTT TTACTGGCAC CCTGCAGTAC ATGGCACCTG AGATAATTGA CCAAGGGCCT 
CGCGGATATG GTGCCCCAGC CGATATCTGG TCCCTGGGCT GCACCATCAT TGAGATGGCC 
ACCAGCAAGC CTCCGTTCCA TGAGCTTGGT GAGCCGCAGG CAGCCATGTT CAAAGTGGGC 
ATGTTTAAGA TCCACCCTGA GATTCCAGAA GCCCTTTCAG CTGAAGCCCG AGCCTTCATT 
TTATCCTGTT TCGAGCCTGA CCCCCACAAA CGTGCCACCA CTGCTGAGCT ACTGAGAGAG 
GGTTTCTTAA GGCAGGTGAA CAAGGGCAAG AAGAACCGAA TTGCCTTCAA GCCCTCAGAA 
GGTCCCCGCG GTGTCGTCCT GGCCCTGCCC ACACAGGGAG AGCCCATGGC CACCAGCAGC 
AGCGAGCACG GCTCTGTCTC CCCAGACTCC GACGCCCAGC CTGACGCACT CTTTGAGAGG 
ACCCGGGCGC CCAGGCACCA CCTTGGCCAC CTCCTCAGTG TTCCAGACGA GAGCTCAGCC 
TTGGAAGACC GGGGCTTGGC CTCGTCCCCG GAGGACAGGG ACCAGGGCCT CTTCCTGCTA 
CGCAAGGACA GTGAGCGCCG TGCCATCCTG TACAAAATCC TCTGGGAGGA GCAGAACCAG 
GTGGCTTCCA ACCTGCAGGA GTGTGTGGCC CAGAGTTCCG AAGAGTTGCA TCTCTCAGTT 
GGACACATCA AGCAAATCAT TGGGATCCTG AGGGACTTCA TCCGCTCCCC AGAGCACCGG 
GTGATGGCGA CCACAATATC AAAGCTCAAG GTGGACCTGG ACTTTGACAG CTCGTCCATC 
AGTCAGATTC ACCTGGTGCT GTTCGGATTT CAGGATGCCG TAAATAAAAT TTTGAGGAAC 
CACTTAATTA GGCCCCACTG GATGTTCGCG ATGGACAACA TCATCCGCCG AGCGGTGCAG 
GCCGCGGTCA CCATTCTCAT CCCAGAGCTC CGAGCCCACT TTGAGCCTAC CTGTGAGACT 
GAAGGGGTAG ATAAGGACAT GGATGAAGCG GAAGAGGGCT ATCCCCCAGC CACCGGACCT 
GGCCAGGAGG CCCAGCCCCA CCAGCAGCAC CTGAGCCTCC AGCTGGGTGA GCTCAGACAG 
GAGACCAACA GACTTTTGGA ACACCTAGTT GAAAAAGAGA GAGAGTACCA GAATCTTCTG 
CGGCAAACTC TAGAACAGAA AACTCAAGAA TTGTATCACC TTCAGTTAAA ATTAAAATCG 
AATTGTATTA CAGAGAACCC AGCAGGCCCC TACGGGCAGA GAACAGATAA AGAGCTTATA 
GACTGGTTGC GGCTGCAAGG AGCTGATGCA AAGACAATTG AAAAGATTGT TGAAGAGGGT 
TATACACTTT CGGATATTCT TAATGAGATC ACTAAGGAAG ATCTAAGATA CCTTCGACTA 
CGGGGTGGTC TCCTCTGCAG ACTCTGGAGT GCGGTCTCCC AGTACAGAAG GGCTCAGGAG 
GCCTCAGAAA CCAAAGACAA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K15 ( XM_011545507.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543809.3 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu80026
Clone ID Related Accession (Same CDS sequence)	XM_011545507.3
Accession Version	XM_011545507.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3597bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGATG TCTCCAGACA GCCTTCCCTC TTCTACCATC TTGGAGTCCG AGAAAGCTTT 
GACATGGCCA ATAATGTGAT CTTGTACCAT GACACCGATG CCGACACTGC TCTCTCTTTG 
AAGGACATGG TAACTCAAAA AAACACAGAA GTCTTTGACC ATAACATCAA CCAATTTCAG 
GCATCCAGTG GAAATTATTA TTTCATCCCA TACATCGTGA CACCGTGCGC TGATTATTTT 
TGCTGCGAGA GTGATGCCCA GAGACGAGCC TCCGAGTACA TGCAGCCCAA CTGGGACAAC 
ATCCTGGGCC CGCTGTGCAT GCCTTTGGTG GACAGGTTCA TTAGCCTCCT TAAGGACATC 
CACGTGACCT CATGTGTTTA TTACAAAGAA ACCTTGTTAA ATGACATCCG GAAAGCCAGA 
GAGAAATACC AAGGTGAGGA ACTGGCGAAG GAGCTAGCTC GGATCAAGCT CCGCATGGAT 
AATACTGAGG TTCTGACCTC AGACATCATC ATTAACTTAC TCCTGTCCTA CCGTGATATC 
CAGGACTATG ATGCGATGGT GAAGCTGGTG GAAACACTGG AGATGCTGCC TACGTGTGAT 
TTGGCCGATC AGCATAACAT TAAATTCCAC TATGCGTTTG CACTGAATAG GAGAAACAGC 
ACAGGTGACC GTGAGAAGGC TCTGCAGATC ATGCTCCAGG TTCTGCAGAG CTGTGATCAC 
CCGGGCCCCG ACATGTTCTG CCTGTGTGGG AGGATCTACA AGGACATCTT CTTGGATTCA 
GACTGCAAAG ATGACACCAG CCGCGACAGC GCCATTGAGT GGTATCGCAA AGGGTTTGAA 
CTCCAGTCAT CCCTCTATTC GGGAATTAAT CTTGCAGTTT TGCTGATTGT TGCTGGACAA 
CAATTTGAAA CTTCCTTGGA ACTAAGGAAA ATAGGTGTCC GGCTGAACAG TTTGTTGGGA 
AGAAAAGGGA GCTTGGAGAA AATGAACAAT TACTGGGATG TGGGTCAGTT CTTCAGCGTC 
AGCATGCTGG CCCATGATGT CGGGAAAGCC GTCCAGGCAG CAGAGAGGTT GTTCAAACTG 
AAACCTCCAG TCTGGTACCT GCGATCATTA GTTCAGAACT TGTTACTAAT TCGGCGCTTC 
AAGAAAACCA TTATTGAACA CTCGCCCAGG CAAGAGCGGC TGAACTTCTG GTTAGATATA 
ATTTTTGAGG CAACAAATGA AGTCACTAAT GGACTCAGAT TTCCAGTTCT GGTCATAGAG 
CCAACCAAAG TGTACCAGCC TTCTTATGTT TCCATAAACA ATGAAGCCGA GGAGAGAACA 
GTTTCTTTAT GGCATGTCTC ACCCACAGAA ATGAAACAGA TGCACGAATG GAATTTTACA 
GCCTCTTCCA TAAAGGGAAT AAGCCTATCA AAGTTTGATG AAAGGTGTTG TTTTCTTTAT 
GTCCATGATA ATTCTGATGA CTTTCAAATC TACTTTTCCA CCGAAGAGCA GTGCAGTAGA 
TTTTTCTCTT TGGTCAAAGA GATGATAACC AATACAGCAG GCAGTACGGT GGAGCTGGAG 
GGAGAGACCG ATGGAGACAC CTTGGAGTAT GAGTATGACC ATGATGCAAA TGGTGAGAGA 
GTTGTCTTGG GGAAAGGCAC GTATGGGATT GTGTATGCTG GCCGAGATCT GAGCAATCAA 
GTGCGAATAG CCATCAAAGA AATCCCGGAG AGAGATAGCA GGTATTCTCA GCCTCTGCAC 
GAGGAGATAG CCCTGCACAA GTACCTTAAG CACCGCAATA TCGTTCAGTA CCTGGGCTCT 
GTTTCAGAGA ACGGCTACAT TAAGATATTT ATGGAGCAGG TGCCTGGAGG AAGCCTTTCT 
GCTCTTCTGC GATCCAAATG GGGGCCGATG AAGGAACCGA CAATCAAGTT TTACACCAAA 
CAGATCCTGG AGGGCCTTAA GTATCTTCAT GAAAACCAGA TCGTGCACAG AGACATAAAG 
GGCGATAATG TTCTGGTGAA CACCTACAGC GGAGTGGTGA AAATCTCCGA TTTTGGAACC 
TCGAAACGTC TTGCGGGTGT GAACCCCTGC ACAGAGACTT TTACTGGCAC CCTGCAGTAC 
ATGGCACCTG AGATAATTGA CCAAGGGCCT CGCGGATATG GTGCCCCAGC CGATATCTGG 
TCCCTGGGCT GCACCATCAT TGAGATGGCC ACCAGCAAGC CTCCGTTCCA TGAGCTTGGT 
GAGCCGCAGG CAGCCATGTT CAAAGTGGGC ATGTTTAAGA TCCACCCTGA GATTCCAGAA 
GCCCTTTCAG CTGAAGCCCG AGCCTTCATT TTATCCTGTT TCGAGCCTGA CCCCCACAAA 
CGTGCCACCA CTGCTGAGCT ACTGAGAGAG GGTTTCTTAA GGCAGGTGAA CAAGGGCAAG 
AAGAACCGAA TTGCCTTCAA GCCCTCAGAA GGTCCCCGCG GTGTCGTCCT GGCCCTGCCC 
ACACAGGGAG AGCCCATGGC CACCAGCAGC AGCGAGCACG GCTCTGTCTC CCCAGACTCC 
GACGCCCAGC CTGACGCACT CTTTGAGAGG ACCCGGGCGC CCAGGCACCA CCTTGGCCAC 
CTCCTCAGTG TTCCAGACGA GAGCTCAGCC TTGGAAGACC GGGGCTTGGC CTCGTCCCCG 
GAGGACAGGG ACCAGGGCCT CTTCCTGCTA CGCAAGGACA GTGAGCGCCG TGCCATCCTG 
TACAAAATCC TCTGGGAGGA GCAGAACCAG GTGGCTTCCA ACCTGCAGGA GTGTGTGGCC 
CAGAGTTCCG AAGAGTTGCA TCTCTCAGTT GGACACATCA AGCAAATCAT TGGGATCCTG 
AGGGACTTCA TCCGCTCCCC AGAGCACCGG GTGATGGCGA CCACAATATC AAAGCTCAAG 
GTGGACCTGG ACTTTGACAG CTCGTCCATC AGTCAGATTC ACCTGGTGCT GTTCGGATTT 
CAGGATGCCG TAAATAAAAT TTTGAGGAAC CACTTAATTA GGCCCCACTG GATGTTCGCG 
ATGGACAACA TCATCCGCCG AGCGGTGCAG GCCGCGGTCA CCATTCTCAT CCCAGAGCTC 
CGAGCCCACT TTGAGCCTAC CTGTGAGACT GAAGGGGTAG ATAAGGACAT GGATGAAGCG 
GAAGAGGGCT ATCCCCCAGC CACCGGACCT GGCCAGGAGG CCCAGCCCCA CCAGCAGCAC 
CTGAGCCTCC AGCTGGGTGA GCTCAGACAG GAGACCAACA GACTTTTGGA ACACCTAGTT 
GAAAAAGAGA GAGAGTACCA GAATCTTCTG CGGCAAACTC TAGAACAGAA AACTCAAGAA 
TTGTATCACC TTCAGTTAAA ATTAAAATCG AATTGTATTA CAGAGAACCC AGCAGGCCCC 
TACGGGCAGA GAACAGATAA AGAGCTTATA GACTGGTTGC GGCTGCAAGG AGCTGATGCA 
AAGACAATTG AAAAGATTGT TGAAGAGGGT TATACACTTT CGGATATTCT TAATGAGATC 
ACTAAGGAAG ATCTAAGATA CCTTCGACTA CGGGGTGGTC TCCTCTGCAG ACTCTGGAGT 
GCGGTCTCCC AGTACAGAAG GGCTCAGGAG GCCTCAGAAA CCAAAGACAA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543809.3
CDS	97..3693
Translation	MSDVSRQPSLFYHLGVRESFDMANNVILYHDTDADTALSLKDMVTQKNTEVFDHNINQFQASSGNYYFIPYIVTPCADYFCCESDAQRRASEYMQPNWDNILGPLCMPLVDRFISLLKDIHVTSCVYYKETLLNDIRKAREKYQGEELAKELARIKLRMDNTEVLTSDIIINLLLSYRDIQDYDAMVKLVETLEMLPTCDLADQHNIKFHYAFALNRRNSTGDREKALQIMLQVLQSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRKGFELQSSLYSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVGQFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGSVSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA

Target ORF information:

RefSeq Version	XM_011545507.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545507.3

ORF Insert Sequence:

ATGAGCGATG TCTCCAGACA GCCTTCCCTC TTCTACCATC TTGGAGTCCG AGAAAGCTTT 
GACATGGCCA ATAATGTGAT CTTGTACCAT GACACCGATG CCGACACTGC TCTCTCTTTG 
AAGGACATGG TAACTCAAAA AAACACAGAA GTCTTTGACC ATAACATCAA CCAATTTCAG 
GCATCCAGTG GAAATTATTA TTTCATCCCA TACATCGTGA CACCGTGCGC TGATTATTTT 
TGCTGCGAGA GTGATGCCCA GAGACGAGCC TCCGAGTACA TGCAGCCCAA CTGGGACAAC 
ATCCTGGGCC CGCTGTGCAT GCCTTTGGTG GACAGGTTCA TTAGCCTCCT TAAGGACATC 
CACGTGACCT CATGTGTTTA TTACAAAGAA ACCTTGTTAA ATGACATCCG GAAAGCCAGA 
GAGAAATACC AAGGTGAGGA ACTGGCGAAG GAGCTAGCTC GGATCAAGCT CCGCATGGAT 
AATACTGAGG TTCTGACCTC AGACATCATC ATTAACTTAC TCCTGTCCTA CCGTGATATC 
CAGGACTATG ATGCGATGGT GAAGCTGGTG GAAACACTGG AGATGCTGCC TACGTGTGAT 
TTGGCCGATC AGCATAACAT TAAATTCCAC TATGCGTTTG CACTGAATAG GAGAAACAGC 
ACAGGTGACC GTGAGAAGGC TCTGCAGATC ATGCTCCAGG TTCTGCAGAG CTGTGATCAC 
CCGGGCCCCG ACATGTTCTG CCTGTGTGGG AGGATCTACA AGGACATCTT CTTGGATTCA 
GACTGCAAAG ATGACACCAG CCGCGACAGC GCCATTGAGT GGTATCGCAA AGGGTTTGAA 
CTCCAGTCAT CCCTCTATTC GGGAATTAAT CTTGCAGTTT TGCTGATTGT TGCTGGACAA 
CAATTTGAAA CTTCCTTGGA ACTAAGGAAA ATAGGTGTCC GGCTGAACAG TTTGTTGGGA 
AGAAAAGGGA GCTTGGAGAA AATGAACAAT TACTGGGATG TGGGTCAGTT CTTCAGCGTC 
AGCATGCTGG CCCATGATGT CGGGAAAGCC GTCCAGGCAG CAGAGAGGTT GTTCAAACTG 
AAACCTCCAG TCTGGTACCT GCGATCATTA GTTCAGAACT TGTTACTAAT TCGGCGCTTC 
AAGAAAACCA TTATTGAACA CTCGCCCAGG CAAGAGCGGC TGAACTTCTG GTTAGATATA 
ATTTTTGAGG CAACAAATGA AGTCACTAAT GGACTCAGAT TTCCAGTTCT GGTCATAGAG 
CCAACCAAAG TGTACCAGCC TTCTTATGTT TCCATAAACA ATGAAGCCGA GGAGAGAACA 
GTTTCTTTAT GGCATGTCTC ACCCACAGAA ATGAAACAGA TGCACGAATG GAATTTTACA 
GCCTCTTCCA TAAAGGGAAT AAGCCTATCA AAGTTTGATG AAAGGTGTTG TTTTCTTTAT 
GTCCATGATA ATTCTGATGA CTTTCAAATC TACTTTTCCA CCGAAGAGCA GTGCAGTAGA 
TTTTTCTCTT TGGTCAAAGA GATGATAACC AATACAGCAG GCAGTACGGT GGAGCTGGAG 
GGAGAGACCG ATGGAGACAC CTTGGAGTAT GAGTATGACC ATGATGCAAA TGGTGAGAGA 
GTTGTCTTGG GGAAAGGCAC GTATGGGATT GTGTATGCTG GCCGAGATCT GAGCAATCAA 
GTGCGAATAG CCATCAAAGA AATCCCGGAG AGAGATAGCA GGTATTCTCA GCCTCTGCAC 
GAGGAGATAG CCCTGCACAA GTACCTTAAG CACCGCAATA TCGTTCAGTA CCTGGGCTCT 
GTTTCAGAGA ACGGCTACAT TAAGATATTT ATGGAGCAGG TGCCTGGAGG AAGCCTTTCT 
GCTCTTCTGC GATCCAAATG GGGGCCGATG AAGGAACCGA CAATCAAGTT TTACACCAAA 
CAGATCCTGG AGGGCCTTAA GTATCTTCAT GAAAACCAGA TCGTGCACAG AGACATAAAG 
GGCGATAATG TTCTGGTGAA CACCTACAGC GGAGTGGTGA AAATCTCCGA TTTTGGAACC 
TCGAAACGTC TTGCGGGTGT GAACCCCTGC ACAGAGACTT TTACTGGCAC CCTGCAGTAC 
ATGGCACCTG AGATAATTGA CCAAGGGCCT CGCGGATATG GTGCCCCAGC CGATATCTGG 
TCCCTGGGCT GCACCATCAT TGAGATGGCC ACCAGCAAGC CTCCGTTCCA TGAGCTTGGT 
GAGCCGCAGG CAGCCATGTT CAAAGTGGGC ATGTTTAAGA TCCACCCTGA GATTCCAGAA 
GCCCTTTCAG CTGAAGCCCG AGCCTTCATT TTATCCTGTT TCGAGCCTGA CCCCCACAAA 
CGTGCCACCA CTGCTGAGCT ACTGAGAGAG GGTTTCTTAA GGCAGGTGAA CAAGGGCAAG 
AAGAACCGAA TTGCCTTCAA GCCCTCAGAA GGTCCCCGCG GTGTCGTCCT GGCCCTGCCC 
ACACAGGGAG AGCCCATGGC CACCAGCAGC AGCGAGCACG GCTCTGTCTC CCCAGACTCC 
GACGCCCAGC CTGACGCACT CTTTGAGAGG ACCCGGGCGC CCAGGCACCA CCTTGGCCAC 
CTCCTCAGTG TTCCAGACGA GAGCTCAGCC TTGGAAGACC GGGGCTTGGC CTCGTCCCCG 
GAGGACAGGG ACCAGGGCCT CTTCCTGCTA CGCAAGGACA GTGAGCGCCG TGCCATCCTG 
TACAAAATCC TCTGGGAGGA GCAGAACCAG GTGGCTTCCA ACCTGCAGGA GTGTGTGGCC 
CAGAGTTCCG AAGAGTTGCA TCTCTCAGTT GGACACATCA AGCAAATCAT TGGGATCCTG 
AGGGACTTCA TCCGCTCCCC AGAGCACCGG GTGATGGCGA CCACAATATC AAAGCTCAAG 
GTGGACCTGG ACTTTGACAG CTCGTCCATC AGTCAGATTC ACCTGGTGCT GTTCGGATTT 
CAGGATGCCG TAAATAAAAT TTTGAGGAAC CACTTAATTA GGCCCCACTG GATGTTCGCG 
ATGGACAACA TCATCCGCCG AGCGGTGCAG GCCGCGGTCA CCATTCTCAT CCCAGAGCTC 
CGAGCCCACT TTGAGCCTAC CTGTGAGACT GAAGGGGTAG ATAAGGACAT GGATGAAGCG 
GAAGAGGGCT ATCCCCCAGC CACCGGACCT GGCCAGGAGG CCCAGCCCCA CCAGCAGCAC 
CTGAGCCTCC AGCTGGGTGA GCTCAGACAG GAGACCAACA GACTTTTGGA ACACCTAGTT 
GAAAAAGAGA GAGAGTACCA GAATCTTCTG CGGCAAACTC TAGAACAGAA AACTCAAGAA 
TTGTATCACC TTCAGTTAAA ATTAAAATCG AATTGTATTA CAGAGAACCC AGCAGGCCCC 
TACGGGCAGA GAACAGATAA AGAGCTTATA GACTGGTTGC GGCTGCAAGG AGCTGATGCA 
AAGACAATTG AAAAGATTGT TGAAGAGGGT TATACACTTT CGGATATTCT TAATGAGATC 
ACTAAGGAAG ATCTAAGATA CCTTCGACTA CGGGGTGGTC TCCTCTGCAG ACTCTGGAGT 
GCGGTCTCCC AGTACAGAAG GGCTCAGGAG GCCTCAGAAA CCAAAGACAA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K15 ( XM_011545508.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543810.3 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu80027
Clone ID Related Accession (Same CDS sequence)	XM_011545508.3
Accession Version	XM_011545508.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGATG TCTCCAGACA GCCTTCCCTC TTCTACCATC TTGGAGTCCG AGAAAGCTTT 
GACATGGCCA ATAATGTGAT CTTGTACCAT GACACCGATG CCGACACTGC TCTCTCTTTG 
AAGGACATGG TAACTCAAAA AAACACAGAA GTCTTTGACC ATAACATCAA CCAATTTCAG 
GCATCCAGTG GAAATTATTA TTTCATCCCA TACATCGTGA CACCGTGCGC TGATTATTTT 
TGCTGCGAGA GTGATGCCCA GAGACGAGCC TCCGAGTACA TGCAGCCCAA CTGGGACAAC 
ATCCTGGGCC CGCTGTGCAT GCCTTTGGTG GACAGGTTCA TTAGCCTCCT TAAGGACATC 
CACGTGACCT CATGTGTTTA TTACAAAGAA ACCTTGTTAA ATGACATCCG GAAAGCCAGA 
GAGAAATACC AAGGTGAGGA ACTGGCGAAG GAGCTAGCTC GGATCAAGCT CCGCATGGAT 
AATACTGAGG TTCTGACCTC AGACATCATC ATTAACTTAC TCCTGTCCTA CCGTGATATC 
CAGGACTATG ATGCGATGGT GAAGCTGGTG GAAACACTGG AGATGCTGCC TACGTGTGAT 
TTGGCCGATC AGCATAACAT TAAATTCCAC TATGCGTTTG CACTGAATAG GAGAAACAGC 
ACAGGTGACC GTGAGAAGGC TCTGCAGATC ATGCTCCAGG TTCTGCAGAG CTGTGATCAC 
CCGGGCCCCG ACATGTTCTG CCTGTGTGGG AGGATCTACA AGGACATCTT CTTGGATTCA 
GACTGCAAAG ATGACACCAG CCGCGACAGC GCCATTGAGT GGTATCGCAA AGGGTTTGAA 
CTCCAGTCAT CCCTCTATTC GGGAATTAAT CTTGCAGTTT TGCTGATTGT TGCTGGACAA 
CAATTTGAAA CTTCCTTGGA ACTAAGGAAA ATAGGTGTCC GGCTGAACAG TTTGTTGGGA 
AGAAAAGGGA GCTTGGAGAA AATGAACAAT TACTGGGATG TGGGTCAGTT CTTCAGCGTC 
AGCATGCTGG CCCATGATGT CGGGAAAGCC GTCCAGGCAG CAGAGAGGTT GTTCAAACTG 
AAACCTCCAG TCTGGTACCT GCGATCATTA GTTCAGAACT TGTTACTAAT TCGGCGCTTC 
AAGAAAACCA TTATTGAACA CTCGCCCAGG CAAGAGCGGC TGAACTTCTG GTTAGATATA 
ATTTTTGAGG CAACAAATGA AGTCACTAAT GGACTCAGAT TTCCAGTTCT GGTCATAGAG 
CCAACCAAAG TGTACCAGCC TTCTTATGTT TCCATAAACA ATGAAGCCGA GGAGAGAACA 
GTTTCTTTAT GGCATGTCTC ACCCACAGAA ATGAAACAGA TGCACGAATG GAATTTTACA 
GCCTCTTCCA TAAAGGGAAT AAGCCTATCA AAGTTTGATG AAAGGTGTTG TTTTCTTTAT 
GTCCATGATA ATTCTGATGA CTTTCAAATC TACTTTTCCA CCGAAGAGCA GTGCAGTAGA 
TTTTTCTCTT TGGTCAAAGA GATGATAACC AATACAGCAG GCAGTACGGT GGAGCTGGAG 
GGAGAGACCG ATGGAGACAC CTTGGAGTAT GAGTATGACC ATGATGCAAA TGGTGAGAGA 
GTTGTCTTGG GGAAAGGCAC GTATGGGATT GTGTATGCTG GCCGAGATCT GAGCAATCAA 
GTGCGAATAG CCATCAAAGA AATCCCGGAG AGAGATAGCA GGTATTCTCA GCCTCTGCAC 
GAGGAGATAG CCCTGCACAA GTACCTTAAG CACCGCAATA TCGTTCAGTA CCTGGGCTCT 
GTTTCAGAGA ACGGCTACAT TAAGATATTT ATGGAGCAGG TGCCTGGAGG AAGCCTTTCT 
GCTCTTCTGC GATCCAAATG GGGGCCGATG AAGGAACCGA CAATCAAGTT TTACACCAAA 
CAGATCCTGG AGGGCCTTAA GTATCTTCAT GAAAACCAGA TCGTGCACAG AGACATAAAG 
GGCGATAATG TTCTGGTGAA CACCTACAGC GGAGTGGTGA AAATCTCCGA TTTTGGAACC 
TCGAAACGTC TTGCGGGTGT GAACCCCTGC ACAGAGACTT TTACTGGCAC CCTGCAGTAC 
ATGGCACCTG AGATAATTGA CCAAGGGCCT CGCGGATATG GTGCCCCAGC CGATATCTGG 
TCCCTGGGCT GCACCATCAT TGAGATGGCC ACCAGCAAGC CTCCGTTCCA TGAGCTTGGT 
GAGCCGCAGG CAGCCATGTT CAAAGTGGGC ATGTTTAAGA TCCACCCTGA GATTCCAGAA 
GCCCTTTCAG CTGAAGCCCG AGCCTTCATT TTATCCTGTT TCGAGCCTGA CCCCCACAAA 
CGTGCCACCA CTGCTGAGCT ACTGAGAGAG GGTTTCTTAA GGCAGGTGAA CAAGGGCAAG 
AAGAACCGAA TTGCCTTCAA GCCCTCAGAC TCCGACGCCC AGCCTGACGC ACTCTTTGAG 
AGGACCCGGG CGCCCAGGCA CCACCTTGGC CACCTCCTCA GTGTTCCAGA CGAGAGCTCA 
GCCTTGGAAG ACCGGGGCTT GGCCTCGTCC CCGGAGGACA GGGACCAGGG CCTCTTCCTG 
CTACGCAAGG ACAGTGAGCG CCGTGCCATC CTGTACAAAA TCCTCTGGGA GGAGCAGAAC 
CAGGTGGCTT CCAACCTGCA GGAGTGTGTG GCCCAGAGTT CCGAAGAGTT GCATCTCTCA 
GTTGGACACA TCAAGCAAAT CATTGGGATC CTGAGGGACT TCATCCGCTC CCCAGAGCAC 
CGGGTGATGG CGACCACAAT ATCAAAGCTC AAGGTGGACC TGGACTTTGA CAGCTCGTCC 
ATCAGTCAGA TTCACCTGGT GCTGTTCGGA TTTCAGGATG CCGTAAATAA AATTTTGAGG 
AACCACTTAA TTAGGCCCCA CTGGATGTTC GCGATGGACA ACATCATCCG CCGAGCGGTG 
CAGGCCGCGG TCACCATTCT CATCCCAGAG CTCCGAGCCC ACTTTGAGCC TACCTGTGAG 
ACTGAAGGGG TAGATAAGGA CATGGATGAA GCGGAAGAGG GCTATCCCCC AGCCACCGGA 
CCTGGCCAGG AGGCCCAGCC CCACCAGCAG CACCTGAGCC TCCAGCTGGG TGAGCTCAGA 
CAGGAGACCA ACAGACTTTT GGAACACCTA GTTGAAAAAG AGAGAGAGTA CCAGAATCTT 
CTGCGGCAAA CTCTAGAACA GAAAACTCAA GAATTGTATC ACCTTCAGTT AAAATTAAAA 
TCGAATTGTA TTACAGAGAA CCCAGCAGGC CCCTACGGGC AGAGAACAGA TAAAGAGCTT 
ATAGACTGGT TGCGGCTGCA AGGAGCTGAT GCAAAGACAA TTGAAAAGAT TGTTGAAGAG 
GGTTATACAC TTTCGGATAT TCTTAATGAG ATCACTAAGG AAGATCTAAG ATACCTTCGA 
CTACGGGGTG GTCTCCTCTG CAGACTCTGG AGTGCGGTCT CCCAGTACAG AAGGGCTCAG 
GAGGCCTCAG AAACCAAAGA CAAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543810.3
CDS	97..3606
Translation	MSDVSRQPSLFYHLGVRESFDMANNVILYHDTDADTALSLKDMVTQKNTEVFDHNINQFQASSGNYYFIPYIVTPCADYFCCESDAQRRASEYMQPNWDNILGPLCMPLVDRFISLLKDIHVTSCVYYKETLLNDIRKAREKYQGEELAKELARIKLRMDNTEVLTSDIIINLLLSYRDIQDYDAMVKLVETLEMLPTCDLADQHNIKFHYAFALNRRNSTGDREKALQIMLQVLQSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRKGFELQSSLYSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVGQFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA

Target ORF information:

RefSeq Version	XM_011545508.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545508.3

ORF Insert Sequence:

ATGAGCGATG TCTCCAGACA GCCTTCCCTC TTCTACCATC TTGGAGTCCG AGAAAGCTTT 
GACATGGCCA ATAATGTGAT CTTGTACCAT GACACCGATG CCGACACTGC TCTCTCTTTG 
AAGGACATGG TAACTCAAAA AAACACAGAA GTCTTTGACC ATAACATCAA CCAATTTCAG 
GCATCCAGTG GAAATTATTA TTTCATCCCA TACATCGTGA CACCGTGCGC TGATTATTTT 
TGCTGCGAGA GTGATGCCCA GAGACGAGCC TCCGAGTACA TGCAGCCCAA CTGGGACAAC 
ATCCTGGGCC CGCTGTGCAT GCCTTTGGTG GACAGGTTCA TTAGCCTCCT TAAGGACATC 
CACGTGACCT CATGTGTTTA TTACAAAGAA ACCTTGTTAA ATGACATCCG GAAAGCCAGA 
GAGAAATACC AAGGTGAGGA ACTGGCGAAG GAGCTAGCTC GGATCAAGCT CCGCATGGAT 
AATACTGAGG TTCTGACCTC AGACATCATC ATTAACTTAC TCCTGTCCTA CCGTGATATC 
CAGGACTATG ATGCGATGGT GAAGCTGGTG GAAACACTGG AGATGCTGCC TACGTGTGAT 
TTGGCCGATC AGCATAACAT TAAATTCCAC TATGCGTTTG CACTGAATAG GAGAAACAGC 
ACAGGTGACC GTGAGAAGGC TCTGCAGATC ATGCTCCAGG TTCTGCAGAG CTGTGATCAC 
CCGGGCCCCG ACATGTTCTG CCTGTGTGGG AGGATCTACA AGGACATCTT CTTGGATTCA 
GACTGCAAAG ATGACACCAG CCGCGACAGC GCCATTGAGT GGTATCGCAA AGGGTTTGAA 
CTCCAGTCAT CCCTCTATTC GGGAATTAAT CTTGCAGTTT TGCTGATTGT TGCTGGACAA 
CAATTTGAAA CTTCCTTGGA ACTAAGGAAA ATAGGTGTCC GGCTGAACAG TTTGTTGGGA 
AGAAAAGGGA GCTTGGAGAA AATGAACAAT TACTGGGATG TGGGTCAGTT CTTCAGCGTC 
AGCATGCTGG CCCATGATGT CGGGAAAGCC GTCCAGGCAG CAGAGAGGTT GTTCAAACTG 
AAACCTCCAG TCTGGTACCT GCGATCATTA GTTCAGAACT TGTTACTAAT TCGGCGCTTC 
AAGAAAACCA TTATTGAACA CTCGCCCAGG CAAGAGCGGC TGAACTTCTG GTTAGATATA 
ATTTTTGAGG CAACAAATGA AGTCACTAAT GGACTCAGAT TTCCAGTTCT GGTCATAGAG 
CCAACCAAAG TGTACCAGCC TTCTTATGTT TCCATAAACA ATGAAGCCGA GGAGAGAACA 
GTTTCTTTAT GGCATGTCTC ACCCACAGAA ATGAAACAGA TGCACGAATG GAATTTTACA 
GCCTCTTCCA TAAAGGGAAT AAGCCTATCA AAGTTTGATG AAAGGTGTTG TTTTCTTTAT 
GTCCATGATA ATTCTGATGA CTTTCAAATC TACTTTTCCA CCGAAGAGCA GTGCAGTAGA 
TTTTTCTCTT TGGTCAAAGA GATGATAACC AATACAGCAG GCAGTACGGT GGAGCTGGAG 
GGAGAGACCG ATGGAGACAC CTTGGAGTAT GAGTATGACC ATGATGCAAA TGGTGAGAGA 
GTTGTCTTGG GGAAAGGCAC GTATGGGATT GTGTATGCTG GCCGAGATCT GAGCAATCAA 
GTGCGAATAG CCATCAAAGA AATCCCGGAG AGAGATAGCA GGTATTCTCA GCCTCTGCAC 
GAGGAGATAG CCCTGCACAA GTACCTTAAG CACCGCAATA TCGTTCAGTA CCTGGGCTCT 
GTTTCAGAGA ACGGCTACAT TAAGATATTT ATGGAGCAGG TGCCTGGAGG AAGCCTTTCT 
GCTCTTCTGC GATCCAAATG GGGGCCGATG AAGGAACCGA CAATCAAGTT TTACACCAAA 
CAGATCCTGG AGGGCCTTAA GTATCTTCAT GAAAACCAGA TCGTGCACAG AGACATAAAG 
GGCGATAATG TTCTGGTGAA CACCTACAGC GGAGTGGTGA AAATCTCCGA TTTTGGAACC 
TCGAAACGTC TTGCGGGTGT GAACCCCTGC ACAGAGACTT TTACTGGCAC CCTGCAGTAC 
ATGGCACCTG AGATAATTGA CCAAGGGCCT CGCGGATATG GTGCCCCAGC CGATATCTGG 
TCCCTGGGCT GCACCATCAT TGAGATGGCC ACCAGCAAGC CTCCGTTCCA TGAGCTTGGT 
GAGCCGCAGG CAGCCATGTT CAAAGTGGGC ATGTTTAAGA TCCACCCTGA GATTCCAGAA 
GCCCTTTCAG CTGAAGCCCG AGCCTTCATT TTATCCTGTT TCGAGCCTGA CCCCCACAAA 
CGTGCCACCA CTGCTGAGCT ACTGAGAGAG GGTTTCTTAA GGCAGGTGAA CAAGGGCAAG 
AAGAACCGAA TTGCCTTCAA GCCCTCAGAC TCCGACGCCC AGCCTGACGC ACTCTTTGAG 
AGGACCCGGG CGCCCAGGCA CCACCTTGGC CACCTCCTCA GTGTTCCAGA CGAGAGCTCA 
GCCTTGGAAG ACCGGGGCTT GGCCTCGTCC CCGGAGGACA GGGACCAGGG CCTCTTCCTG 
CTACGCAAGG ACAGTGAGCG CCGTGCCATC CTGTACAAAA TCCTCTGGGA GGAGCAGAAC 
CAGGTGGCTT CCAACCTGCA GGAGTGTGTG GCCCAGAGTT CCGAAGAGTT GCATCTCTCA 
GTTGGACACA TCAAGCAAAT CATTGGGATC CTGAGGGACT TCATCCGCTC CCCAGAGCAC 
CGGGTGATGG CGACCACAAT ATCAAAGCTC AAGGTGGACC TGGACTTTGA CAGCTCGTCC 
ATCAGTCAGA TTCACCTGGT GCTGTTCGGA TTTCAGGATG CCGTAAATAA AATTTTGAGG 
AACCACTTAA TTAGGCCCCA CTGGATGTTC GCGATGGACA ACATCATCCG CCGAGCGGTG 
CAGGCCGCGG TCACCATTCT CATCCCAGAG CTCCGAGCCC ACTTTGAGCC TACCTGTGAG 
ACTGAAGGGG TAGATAAGGA CATGGATGAA GCGGAAGAGG GCTATCCCCC AGCCACCGGA 
CCTGGCCAGG AGGCCCAGCC CCACCAGCAG CACCTGAGCC TCCAGCTGGG TGAGCTCAGA 
CAGGAGACCA ACAGACTTTT GGAACACCTA GTTGAAAAAG AGAGAGAGTA CCAGAATCTT 
CTGCGGCAAA CTCTAGAACA GAAAACTCAA GAATTGTATC ACCTTCAGTT AAAATTAAAA 
TCGAATTGTA TTACAGAGAA CCCAGCAGGC CCCTACGGGC AGAGAACAGA TAAAGAGCTT 
ATAGACTGGT TGCGGCTGCA AGGAGCTGAT GCAAAGACAA TTGAAAAGAT TGTTGAAGAG 
GGTTATACAC TTTCGGATAT TCTTAATGAG ATCACTAAGG AAGATCTAAG ATACCTTCGA 
CTACGGGGTG GTCTCCTCTG CAGACTCTGG AGTGCGGTCT CCCAGTACAG AAGGGCTCAG 
GAGGCCTCAG AAACCAAAGA CAAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K15 ( NM_001001671.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001671.3 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), mRNA.

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CloneID	OHu21561
Clone ID Related Accession (Same CDS sequence)	NM_001001671.3
Accession Version	NM_001001671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3942bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL732423.12, DV080548.1, AK131412.1 and CN293540.1. This sequence is a reference standard in the RefSeqGene project. On Jan 1, 2010 this sequence version replaced NM_001001671.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments, including the related human protein NP_005914.1, but the complete splice pattern has not been experimentally verified. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGCATC CAGTGGAAAT 
TATTATTTCA TCCCATACAT CGTGACACCG TGCGCTGATT ATTTTTGCTG CGAGAGTGAT 
GCCCAGAGAC GAGCCTCCGA GTACATGCAG CCCAACTGGG ACAACATCCT GGGCCCGCTG 
TGCATGCCTT TGGTGGACAG GTTCATTAGC CTCCTTAAGG ACATCCACGT GACCTCATGT 
GTTTATTACA AAGAAACCTT GTTAAATGAC ATCCGGAAAG CCAGAGAGAA ATACCAAGGT 
GAGGAACTGG CGAAGGAGCT AGCTCGGATC AAGCTCCGCA TGGATAATAC TGAGGTTCTG 
ACCTCAGACA TCATCATTAA CTTACTCCTG TCCTACCGTG ATATCCAGGA CTATGATGCG 
ATGGTGAAGC TGGTGGAAAC ACTGGAGATG CTGCCTACGT GTGATTTGGC CGATCAGCAT 
AACATTAAAT TCCACTATGC GTTTGCACTG AATAGGAGAA ACAGCACAGG TGACCGTGAG 
AAGGCTCTGC AGATCATGCT CCAGGTTCTG CAGAGCTGTG ATCACCCGGG CCCCGACATG 
TTCTGCCTGT GTGGGAGGAT CTACAAGGAC ATCTTCTTGG ATTCAGACTG CAAAGATGAC 
ACCAGCCGCG ACAGCGCCAT TGAGTGGTAT CGCAAAGGGT TTGAACTCCA GTCATCCCTC 
TATTCGGGAA TTAATCTTGC AGTTTTGCTG ATTGTTGCTG GACAACAATT TGAAACTTCC 
TTGGAACTAA GGAAAATAGG TGTCCGGCTG AACAGTTTGT TGGGAAGAAA AGGGAGCTTG 
GAGAAAATGA ACAATTACTG GGATGTGGGT CAGTTCTTCA GCGTCAGCAT GCTGGCCCAT 
GATGTCGGGA AAGCCGTCCA GGCAGCAGAG AGGTTGTTCA AACTGAAACC TCCAGTCTGG 
TACCTGCGAT CATTAGTTCA GAACTTGTTA CTAATTCGGC GCTTCAAGAA AACCATTATT 
GAACACTCGC CCAGGCAAGA GCGGCTGAAC TTCTGGTTAG ATATAATTTT TGAGGCAACA 
AATGAAGTCA CTAATGGACT CAGATTTCCA GTTCTGGTCA TAGAGCCAAC CAAAGTGTAC 
CAGCCTTCTT ATGTTTCCAT AAACAATGAA GCCGAGGAGA GAACAGTTTC TTTATGGCAT 
GTCTCACCCA CAGAAATGAA ACAGATGCAC GAATGGAATT TTACAGCCTC TTCCATAAAG 
GGAATAAGCC TATCAAAGTT TGATGAAAGG TGTTGTTTTC TTTATGTCCA TGATAATTCT 
GATGACTTTC AAATCTACTT TTCCACCGAA GAGCAGTGCA GTAGATTTTT CTCTTTGGTC 
AAAGAGATGA TAACCAATAC AGCAGGCAGT ACGGTGGAGC TGGAGGGAGA GACCGATGGA 
GACACCTTGG AGTATGAGTA TGACCATGAT GCAAATGGTG AGAGAGTTGT CTTGGGGAAA 
GGCACGTATG GGATTGTGTA TGCTGGCCGA GATCTGAGCA ATCAAGTGCG AATAGCCATC 
AAAGAAATCC CGGAGAGAGA TAGCAGGTAT TCTCAGCCTC TGCACGAGGA GATAGCCCTG 
CACAAGTACC TTAAGCACCG CAATATCGTT CAGTACCTGG GCTCTGTTTC AGAGAACGGC 
TACATTAAGA TATTTATGGA GCAGGTGCCT GGAGGAAGCC TTTCTGCTCT TCTGCGATCC 
AAATGGGGGC CGATGAAGGA ACCGACAATC AAGTTTTACA CCAAACAGAT CCTGGAGGGC 
CTTAAGTATC TTCATGAAAA CCAGATCGTG CACAGAGACA TAAAGGGCGA TAATGTTCTG 
GTGAACACCT ACAGCGGAGT GGTGAAAATC TCCGATTTTG GAACCTCGAA ACGTCTTGCG 
GGTGTGAACC CCTGCACAGA GACTTTTACT GGCACCCTGC AGTACATGGC ACCTGAGATA 
ATTGACCAAG GGCCTCGCGG ATATGGTGCC CCAGCCGATA TCTGGTCCCT GGGCTGCACC 
ATCATTGAGA TGGCCACCAG CAAGCCTCCG TTCCATGAGC TTGGTGAGCC GCAGGCAGCC 
ATGTTCAAAG TGGGCATGTT TAAGATCCAC CCTGAGATTC CAGAAGCCCT TTCAGCTGAA 
GCCCGAGCCT TCATTTTATC CTGTTTCGAG CCTGACCCCC ACAAACGTGC CACCACTGCT 
GAGCTACTGA GAGAGGGTTT CTTAAGGCAG GTGAACAAGG GCAAGAAGAA CCGAATTGCC 
TTCAAGCCCT CAGAAGGTCC CCGCGGTGTC GTCCTGGCCC TGCCCACACA GGGAGAGCCC 
ATGGCCACCA GCAGCAGCGA GCACGGCTCT GTCTCCCCAG ACTCCGACGC CCAGCCTGAC 
GCACTCTTTG AGAGGACCCG GGCGCCCAGG CACCACCTTG GCCACCTCCT CAGTGTTCCA 
GACGAGAGCT CAGCCTTGGA AGACCGGGGC TTGGCCTCGT CCCCGGAGGA CAGGGACCAG 
GGCCTCTTCC TGCTACGCAA GGACAGTGAG CGCCGTGCCA TCCTGTACAA AATCCTCTGG 
GAGGAGCAGA ACCAGGTGGC TTCCAACCTG CAGGAGTGTG TGGCCCAGAG TTCCGAAGAG 
TTGCATCTCT CAGTTGGACA CATCAAGCAA ATCATTGGGA TCCTGAGGGA CTTCATCCGC 
TCCCCAGAGC ACCGGGTGAT GGCGACCACA ATATCAAAGC TCAAGGTGGA CCTGGACTTT 
GACAGCTCGT CCATCAGTCA GATTCACCTG GTGCTGTTCG GATTTCAGGA TGCCGTAAAT 
AAAATTTTGA GGAACCACTT AATTAGGCCC CACTGGATGT TCGCGATGGA CAACATCATC 
CGCCGAGCGG TGCAGGCCGC GGTCACCATT CTCATCCCAG AGCTCCGAGC CCACTTTGAG 
CCTACCTGTG AGACTGAAGG GGTAGATAAG GACATGGATG AAGCGGAAGA GGGCTATCCC 
CCAGCCACCG GACCTGGCCA GGAGGCCCAG CCCCACCAGC AGCACCTGAG CCTCCAGCTG 
GGTGAGCTCA GACAGGAGAC CAACAGACTT TTGGAACACC TAGTTGAAAA AGAGAGAGAG 
TACCAGAATC TTCTGCGGCA AACTCTAGAA CAGAAAACTC AAGAATTGTA TCACCTTCAG 
TTAAAATTAA AATCGAATTG TATTACAGAG AACCCAGCAG GCCCCTACGG GCAGAGAACA 
GATAAAGAGC TTATAGACTG GTTGCGGCTG CAAGGAGCTG ATGCAAAGAC AATTGAAAAG 
ATTGTTGAAG AGGGTTATAC ACTTTCGGAT ATTCTTAATG AGATCACTAA GGAAGATCTA 
AGATACCTTC GACTACGGGG TGGTCTCCTC TGCAGACTCT GGAGTGCGGT CTCCCAGTAC 
AGAAGGGCTC AGGAGGCCTC AGAAACCAAA GACAAGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001671.3
CDS	1..3942
Translation	MESGGGNAPAGALGAASESPQCPPPPGVEGAAGPAEPDGAAEGAAGGSGEGESGGGPRRALRAVYVRSESSQGGAAGGPEAGARQCLLRACEAEGAHLTSVPFGELDFGETAVLDAFYDADVAVVDMSDVSRQPSLFYHLGVRESFDMANNVILYHDTDADTALSLKDMVTQKNTASSGNYYFIPYIVTPCADYFCCESDAQRRASEYMQPNWDNILGPLCMPLVDRFISLLKDIHVTSCVYYKETLLNDIRKAREKYQGEELAKELARIKLRMDNTEVLTSDIIINLLLSYRDIQDYDAMVKLVETLEMLPTCDLADQHNIKFHYAFALNRRNSTGDREKALQIMLQVLQSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRKGFELQSSLYSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVGQFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGSVSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA

Target ORF information:

RefSeq Version	NM_001001671.3
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001671.3

ORF Insert Sequence:

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGCATC CAGTGGAAAT 
TATTATTTCA TCCCATACAT CGTGACACCG TGCGCTGATT ATTTTTGCTG CGAGAGTGAT 
GCCCAGAGAC GAGCCTCCGA GTACATGCAG CCCAACTGGG ACAACATCCT GGGCCCGCTG 
TGCATGCCTT TGGTGGACAG GTTCATTAGC CTCCTTAAGG ACATCCACGT GACCTCATGT 
GTTTATTACA AAGAAACCTT GTTAAATGAC ATCCGGAAAG CCAGAGAGAA ATACCAAGGT 
GAGGAACTGG CGAAGGAGCT AGCTCGGATC AAGCTCCGCA TGGATAATAC TGAGGTTCTG 
ACCTCAGACA TCATCATTAA CTTACTCCTG TCCTACCGTG ATATCCAGGA CTATGATGCG 
ATGGTGAAGC TGGTGGAAAC ACTGGAGATG CTGCCTACGT GTGATTTGGC CGATCAGCAT 
AACATTAAAT TCCACTATGC GTTTGCACTG AATAGGAGAA ACAGCACAGG TGACCGTGAG 
AAGGCTCTGC AGATCATGCT CCAGGTTCTG CAGAGCTGTG ATCACCCGGG CCCCGACATG 
TTCTGCCTGT GTGGGAGGAT CTACAAGGAC ATCTTCTTGG ATTCAGACTG CAAAGATGAC 
ACCAGCCGCG ACAGCGCCAT TGAGTGGTAT CGCAAAGGGT TTGAACTCCA GTCATCCCTC 
TATTCGGGAA TTAATCTTGC AGTTTTGCTG ATTGTTGCTG GACAACAATT TGAAACTTCC 
TTGGAACTAA GGAAAATAGG TGTCCGGCTG AACAGTTTGT TGGGAAGAAA AGGGAGCTTG 
GAGAAAATGA ACAATTACTG GGATGTGGGT CAGTTCTTCA GCGTCAGCAT GCTGGCCCAT 
GATGTCGGGA AAGCCGTCCA GGCAGCAGAG AGGTTGTTCA AACTGAAACC TCCAGTCTGG 
TACCTGCGAT CATTAGTTCA GAACTTGTTA CTAATTCGGC GCTTCAAGAA AACCATTATT 
GAACACTCGC CCAGGCAAGA GCGGCTGAAC TTCTGGTTAG ATATAATTTT TGAGGCAACA 
AATGAAGTCA CTAATGGACT CAGATTTCCA GTTCTGGTCA TAGAGCCAAC CAAAGTGTAC 
CAGCCTTCTT ATGTTTCCAT AAACAATGAA GCCGAGGAGA GAACAGTTTC TTTATGGCAT 
GTCTCACCCA CAGAAATGAA ACAGATGCAC GAATGGAATT TTACAGCCTC TTCCATAAAG 
GGAATAAGCC TATCAAAGTT TGATGAAAGG TGTTGTTTTC TTTATGTCCA TGATAATTCT 
GATGACTTTC AAATCTACTT TTCCACCGAA GAGCAGTGCA GTAGATTTTT CTCTTTGGTC 
AAAGAGATGA TAACCAATAC AGCAGGCAGT ACGGTGGAGC TGGAGGGAGA GACCGATGGA 
GACACCTTGG AGTATGAGTA TGACCATGAT GCAAATGGTG AGAGAGTTGT CTTGGGGAAA 
GGCACGTATG GGATTGTGTA TGCTGGCCGA GATCTGAGCA ATCAAGTGCG AATAGCCATC 
AAAGAAATCC CGGAGAGAGA TAGCAGGTAT TCTCAGCCTC TGCACGAGGA GATAGCCCTG 
CACAAGTACC TTAAGCACCG CAATATCGTT CAGTACCTGG GCTCTGTTTC AGAGAACGGC 
TACATTAAGA TATTTATGGA GCAGGTGCCT GGAGGAAGCC TTTCTGCTCT TCTGCGATCC 
AAATGGGGGC CGATGAAGGA ACCGACAATC AAGTTTTACA CCAAACAGAT CCTGGAGGGC 
CTTAAGTATC TTCATGAAAA CCAGATCGTG CACAGAGACA TAAAGGGCGA TAATGTTCTG 
GTGAACACCT ACAGCGGAGT GGTGAAAATC TCCGATTTTG GAACCTCGAA ACGTCTTGCG 
GGTGTGAACC CCTGCACAGA GACTTTTACT GGCACCCTGC AGTACATGGC ACCTGAGATA 
ATTGACCAAG GGCCTCGCGG ATATGGTGCC CCAGCCGATA TCTGGTCCCT GGGCTGCACC 
ATCATTGAGA TGGCCACCAG CAAGCCTCCG TTCCATGAGC TTGGTGAGCC GCAGGCAGCC 
ATGTTCAAAG TGGGCATGTT TAAGATCCAC CCTGAGATTC CAGAAGCCCT TTCAGCTGAA 
GCCCGAGCCT TCATTTTATC CTGTTTCGAG CCTGACCCCC ACAAACGTGC CACCACTGCT 
GAGCTACTGA GAGAGGGTTT CTTAAGGCAG GTGAACAAGG GCAAGAAGAA CCGAATTGCC 
TTCAAGCCCT CAGAAGGTCC CCGCGGTGTC GTCCTGGCCC TGCCCACACA GGGAGAGCCC 
ATGGCCACCA GCAGCAGCGA GCACGGCTCT GTCTCCCCAG ACTCCGACGC CCAGCCTGAC 
GCACTCTTTG AGAGGACCCG GGCGCCCAGG CACCACCTTG GCCACCTCCT CAGTGTTCCA 
GACGAGAGCT CAGCCTTGGA AGACCGGGGC TTGGCCTCGT CCCCGGAGGA CAGGGACCAG 
GGCCTCTTCC TGCTACGCAA GGACAGTGAG CGCCGTGCCA TCCTGTACAA AATCCTCTGG 
GAGGAGCAGA ACCAGGTGGC TTCCAACCTG CAGGAGTGTG TGGCCCAGAG TTCCGAAGAG 
TTGCATCTCT CAGTTGGACA CATCAAGCAA ATCATTGGGA TCCTGAGGGA CTTCATCCGC 
TCCCCAGAGC ACCGGGTGAT GGCGACCACA ATATCAAAGC TCAAGGTGGA CCTGGACTTT 
GACAGCTCGT CCATCAGTCA GATTCACCTG GTGCTGTTCG GATTTCAGGA TGCCGTAAAT 
AAAATTTTGA GGAACCACTT AATTAGGCCC CACTGGATGT TCGCGATGGA CAACATCATC 
CGCCGAGCGG TGCAGGCCGC GGTCACCATT CTCATCCCAG AGCTCCGAGC CCACTTTGAG 
CCTACCTGTG AGACTGAAGG GGTAGATAAG GACATGGATG AAGCGGAAGA GGGCTATCCC 
CCAGCCACCG GACCTGGCCA GGAGGCCCAG CCCCACCAGC AGCACCTGAG CCTCCAGCTG 
GGTGAGCTCA GACAGGAGAC CAACAGACTT TTGGAACACC TAGTTGAAAA AGAGAGAGAG 
TACCAGAATC TTCTGCGGCA AACTCTAGAA CAGAAAACTC AAGAATTGTA TCACCTTCAG 
TTAAAATTAA AATCGAATTG TATTACAGAG AACCCAGCAG GCCCCTACGG GCAGAGAACA 
GATAAAGAGC TTATAGACTG GTTGCGGCTG CAAGGAGCTG ATGCAAAGAC AATTGAAAAG 
ATTGTTGAAG AGGGTTATAC ACTTTCGGAT ATTCTTAATG AGATCACTAA GGAAGATCTA 
AGATACCTTC GACTACGGGG TGGTCTCCTC TGCAGACTCT GGAGTGCGGT CTCCCAGTAC 
AGAAGGGCTC AGGAGGCCTC AGAAACCAAA GACAAGGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K15 ( XM_011545507.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543809.2 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu99304
Clone ID Related Accession (Same CDS sequence)	XM_011545507.2
Accession Version	XM_011545507.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3975bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545507.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGAAGT CTTTGACCAT 
AACATCAACC AATTTCAGGC ATCCAGTGGA AATTATTATT TCATCCCATA CATCGTGACA 
CCGTGCGCTG ATTATTTTTG CTGCGAGAGT GATGCCCAGA GACGAGCCTC CGAGTACATG 
CAGCCCAACT GGGACAACAT CCTGGGCCCG CTGTGCATGC CTTTGGTGGA CAGGTTCATT 
AGCCTCCTTA AGGACATCCA CGTGACCTCA TGTGTTTATT ACAAAGAAAC CTTGTTAAAT 
GACATCCGGA AAGCCAGAGA GAAATACCAA GGTGAGGAAC TGGCGAAGGA GCTAGCTCGG 
ATCAAGCTCC GCATGGATAA TACTGAGGTT CTGACCTCAG ACATCATCAT TAACTTACTC 
CTGTCCTACC GTGATATCCA GGACTATGAT GCGATGGTGA AGCTGGTGGA AACACTGGAG 
ATGCTGCCTA CGTGTGATTT GGCCGATCAG CATAACATTA AATTCCACTA TGCGTTTGCA 
CTGAATAGGA GAAACAGCAC AGGTGACCGT GAGAAGGCTC TGCAGATCAT GCTCCAGGTT 
CTGCAGAGCT GTGATCACCC GGGCCCCGAC ATGTTCTGCC TGTGTGGGAG GATCTACAAG 
GACATCTTCT TGGATTCAGA CTGCAAAGAT GACACCAGCC GCGACAGCGC CATTGAGTGG 
TATCGCAAAG GGTTTGAACT CCAGTCATCC CTCTATTCGG GAATTAATCT TGCAGTTTTG 
CTGATTGTTG CTGGACAACA ATTTGAAACT TCCTTGGAAC TAAGGAAAAT AGGTGTCCGG 
CTGAACAGTT TGTTGGGAAG AAAAGGGAGC TTGGAGAAAA TGAACAATTA CTGGGATGTG 
GGTCAGTTCT TCAGCGTCAG CATGCTGGCC CATGATGTCG GGAAAGCCGT CCAGGCAGCA 
GAGAGGTTGT TCAAACTGAA ACCTCCAGTC TGGTACCTGC GATCATTAGT TCAGAACTTG 
TTACTAATTC GGCGCTTCAA GAAAACCATT ATTGAACACT CGCCCAGGCA AGAGCGGCTG 
AACTTCTGGT TAGATATAAT TTTTGAGGCA ACAAATGAAG TCACTAATGG ACTCAGATTT 
CCAGTTCTGG TCATAGAGCC AACCAAAGTG TACCAGCCTT CTTATGTTTC CATAAACAAT 
GAAGCCGAGG AGAGAACAGT TTCTTTATGG CATGTCTCAC CCACAGAAAT GAAACAGATG 
CACGAATGGA ATTTTACAGC CTCTTCCATA AAGGGAATAA GCCTATCAAA GTTTGATGAA 
AGGTGTTGTT TTCTTTATGT CCATGATAAT TCTGATGACT TTCAAATCTA CTTTTCCACC 
GAAGAGCAGT GCAGTAGATT TTTCTCTTTG GTCAAAGAGA TGATAACCAA TACAGCAGGC 
AGTACGGTGG AGCTGGAGGG AGAGACCGAT GGAGACACCT TGGAGTATGA GTATGACCAT 
GATGCAAATG GTGAGAGAGT TGTCTTGGGG AAAGGCACGT ATGGGATTGT GTATGCTGGC 
CGAGATCTGA GCAATCAAGT GCGAATAGCC ATCAAAGAAA TCCCGGAGAG AGATAGCAGG 
TATTCTCAGC CTCTGCACGA GGAGATAGCC CTGCACAAGT ACCTTAAGCA CCGCAATATC 
GTTCAGTACC TGGGCTCTGT TTCAGAGAAC GGCTACATTA AGATATTTAT GGAGCAGGTG 
CCTGGAGGAA GCCTTTCTGC TCTTCTGCGA TCCAAATGGG GGCCGATGAA GGAACCGACA 
ATCAAGTTTT ACACCAAACA GATCCTGGAG GGCCTTAAGT ATCTTCATGA AAACCAGATC 
GTGCACAGAG ACATAAAGGG CGATAATGTT CTGGTGAACA CCTACAGCGG AGTGGTGAAA 
ATCTCCGATT TTGGAACCTC GAAACGTCTT GCGGGTGTGA ACCCCTGCAC AGAGACTTTT 
ACTGGCACCC TGCAGTACAT GGCACCTGAG ATAATTGACC AAGGGCCTCG CGGATATGGT 
GCCCCAGCCG ATATCTGGTC CCTGGGCTGC ACCATCATTG AGATGGCCAC CAGCAAGCCT 
CCGTTCCATG AGCTTGGTGA GCCGCAGGCA GCCATGTTCA AAGTGGGCAT GTTTAAGATC 
CACCCTGAGA TTCCAGAAGC CCTTTCAGCT GAAGCCCGAG CCTTCATTTT ATCCTGTTTC 
GAGCCTGACC CCCACAAACG TGCCACCACT GCTGAGCTAC TGAGAGAGGG TTTCTTAAGG 
CAGGTGAACA AGGGCAAGAA GAACCGAATT GCCTTCAAGC CCTCAGAAGG TCCCCGCGGT 
GTCGTCCTGG CCCTGCCCAC ACAGGGAGAG CCCATGGCCA CCAGCAGCAG CGAGCACGGC 
TCTGTCTCCC CAGACTCCGA CGCCCAGCCT GACGCACTCT TTGAGAGGAC CCGGGCGCCC 
AGGCACCACC TTGGCCACCT CCTCAGTGTT CCAGACGAGA GCTCAGCCTT GGAAGACCGG 
GGCTTGGCCT CGTCCCCGGA GGACAGGGAC CAGGGCCTCT TCCTGCTACG CAAGGACAGT 
GAGCGCCGTG CCATCCTGTA CAAAATCCTC TGGGAGGAGC AGAACCAGGT GGCTTCCAAC 
CTGCAGGAGT GTGTGGCCCA GAGTTCCGAA GAGTTGCATC TCTCAGTTGG ACACATCAAG 
CAAATCATTG GGATCCTGAG GGACTTCATC CGCTCCCCAG AGCACCGGGT GATGGCGACC 
ACAATATCAA AGCTCAAGGT GGACCTGGAC TTTGACAGCT CGTCCATCAG TCAGATTCAC 
CTGGTGCTGT TCGGATTTCA GGATGCCGTA AATAAAATTT TGAGGAACCA CTTAATTAGG 
CCCCACTGGA TGTTCGCGAT GGACAACATC ATCCGCCGAG CGGTGCAGGC CGCGGTCACC 
ATTCTCATCC CAGAGCTCCG AGCCCACTTT GAGCCTACCT GTGAGACTGA AGGGGTAGAT 
AAGGACATGG ATGAAGCGGA AGAGGGCTAT CCCCCAGCCA CCGGACCTGG CCAGGAGGCC 
CAGCCCCACC AGCAGCACCT GAGCCTCCAG CTGGGTGAGC TCAGACAGGA GACCAACAGA 
CTTTTGGAAC ACCTAGTTGA AAAAGAGAGA GAGTACCAGA ATCTTCTGCG GCAAACTCTA 
GAACAGAAAA CTCAAGAATT GTATCACCTT CAGTTAAAAT TAAAATCGAA TTGTATTACA 
GAGAACCCAG CAGGCCCCTA CGGGCAGAGA ACAGATAAAG AGCTTATAGA CTGGTTGCGG 
CTGCAAGGAG CTGATGCAAA GACAATTGAA AAGATTGTTG AAGAGGGTTA TACACTTTCG 
GATATTCTTA ATGAGATCAC TAAGGAAGAT CTAAGATACC TTCGACTACG GGGTGGTCTC 
CTCTGCAGAC TCTGGAGTGC GGTCTCCCAG TACAGAAGGG CTCAGGAGGC CTCAGAAACC 
AAAGACAAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543809.2
CDS	195..4169
Translation	MESGGGNAPAGALGAASESPQCPPPPGVEGAAGPAEPDGAAEGAAGGSGEGESGGGPRRALRAVYVRSESSQGGAAGGPEAGARQCLLRACEAEGAHLTSVPFGELDFGETAVLDAFYDADVAVVDMSDVSRQPSLFYHLGVRESFDMANNVILYHDTDADTALSLKDMVTQKNTEVFDHNINQFQASSGNYYFIPYIVTPCADYFCCESDAQRRASEYMQPNWDNILGPLCMPLVDRFISLLKDIHVTSCVYYKETLLNDIRKAREKYQGEELAKELARIKLRMDNTEVLTSDIIINLLLSYRDIQDYDAMVKLVETLEMLPTCDLADQHNIKFHYAFALNRRNSTGDREKALQIMLQVLQSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRKGFELQSSLYSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVGQFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSEGPRGVVLALPTQGEPMATSSSEHGSVSPDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA

Target ORF information:

RefSeq Version	XM_011545507.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545507.2

ORF Insert Sequence:

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGAAGT CTTTGACCAT 
AACATCAACC AATTTCAGGC ATCCAGTGGA AATTATTATT TCATCCCATA CATCGTGACA 
CCGTGCGCTG ATTATTTTTG CTGCGAGAGT GATGCCCAGA GACGAGCCTC CGAGTACATG 
CAGCCCAACT GGGACAACAT CCTGGGCCCG CTGTGCATGC CTTTGGTGGA CAGGTTCATT 
AGCCTCCTTA AGGACATCCA CGTGACCTCA TGTGTTTATT ACAAAGAAAC CTTGTTAAAT 
GACATCCGGA AAGCCAGAGA GAAATACCAA GGTGAGGAAC TGGCGAAGGA GCTAGCTCGG 
ATCAAGCTCC GCATGGATAA TACTGAGGTT CTGACCTCAG ACATCATCAT TAACTTACTC 
CTGTCCTACC GTGATATCCA GGACTATGAT GCGATGGTGA AGCTGGTGGA AACACTGGAG 
ATGCTGCCTA CGTGTGATTT GGCCGATCAG CATAACATTA AATTCCACTA TGCGTTTGCA 
CTGAATAGGA GAAACAGCAC AGGTGACCGT GAGAAGGCTC TGCAGATCAT GCTCCAGGTT 
CTGCAGAGCT GTGATCACCC GGGCCCCGAC ATGTTCTGCC TGTGTGGGAG GATCTACAAG 
GACATCTTCT TGGATTCAGA CTGCAAAGAT GACACCAGCC GCGACAGCGC CATTGAGTGG 
TATCGCAAAG GGTTTGAACT CCAGTCATCC CTCTATTCGG GAATTAATCT TGCAGTTTTG 
CTGATTGTTG CTGGACAACA ATTTGAAACT TCCTTGGAAC TAAGGAAAAT AGGTGTCCGG 
CTGAACAGTT TGTTGGGAAG AAAAGGGAGC TTGGAGAAAA TGAACAATTA CTGGGATGTG 
GGTCAGTTCT TCAGCGTCAG CATGCTGGCC CATGATGTCG GGAAAGCCGT CCAGGCAGCA 
GAGAGGTTGT TCAAACTGAA ACCTCCAGTC TGGTACCTGC GATCATTAGT TCAGAACTTG 
TTACTAATTC GGCGCTTCAA GAAAACCATT ATTGAACACT CGCCCAGGCA AGAGCGGCTG 
AACTTCTGGT TAGATATAAT TTTTGAGGCA ACAAATGAAG TCACTAATGG ACTCAGATTT 
CCAGTTCTGG TCATAGAGCC AACCAAAGTG TACCAGCCTT CTTATGTTTC CATAAACAAT 
GAAGCCGAGG AGAGAACAGT TTCTTTATGG CATGTCTCAC CCACAGAAAT GAAACAGATG 
CACGAATGGA ATTTTACAGC CTCTTCCATA AAGGGAATAA GCCTATCAAA GTTTGATGAA 
AGGTGTTGTT TTCTTTATGT CCATGATAAT TCTGATGACT TTCAAATCTA CTTTTCCACC 
GAAGAGCAGT GCAGTAGATT TTTCTCTTTG GTCAAAGAGA TGATAACCAA TACAGCAGGC 
AGTACGGTGG AGCTGGAGGG AGAGACCGAT GGAGACACCT TGGAGTATGA GTATGACCAT 
GATGCAAATG GTGAGAGAGT TGTCTTGGGG AAAGGCACGT ATGGGATTGT GTATGCTGGC 
CGAGATCTGA GCAATCAAGT GCGAATAGCC ATCAAAGAAA TCCCGGAGAG AGATAGCAGG 
TATTCTCAGC CTCTGCACGA GGAGATAGCC CTGCACAAGT ACCTTAAGCA CCGCAATATC 
GTTCAGTACC TGGGCTCTGT TTCAGAGAAC GGCTACATTA AGATATTTAT GGAGCAGGTG 
CCTGGAGGAA GCCTTTCTGC TCTTCTGCGA TCCAAATGGG GGCCGATGAA GGAACCGACA 
ATCAAGTTTT ACACCAAACA GATCCTGGAG GGCCTTAAGT ATCTTCATGA AAACCAGATC 
GTGCACAGAG ACATAAAGGG CGATAATGTT CTGGTGAACA CCTACAGCGG AGTGGTGAAA 
ATCTCCGATT TTGGAACCTC GAAACGTCTT GCGGGTGTGA ACCCCTGCAC AGAGACTTTT 
ACTGGCACCC TGCAGTACAT GGCACCTGAG ATAATTGACC AAGGGCCTCG CGGATATGGT 
GCCCCAGCCG ATATCTGGTC CCTGGGCTGC ACCATCATTG AGATGGCCAC CAGCAAGCCT 
CCGTTCCATG AGCTTGGTGA GCCGCAGGCA GCCATGTTCA AAGTGGGCAT GTTTAAGATC 
CACCCTGAGA TTCCAGAAGC CCTTTCAGCT GAAGCCCGAG CCTTCATTTT ATCCTGTTTC 
GAGCCTGACC CCCACAAACG TGCCACCACT GCTGAGCTAC TGAGAGAGGG TTTCTTAAGG 
CAGGTGAACA AGGGCAAGAA GAACCGAATT GCCTTCAAGC CCTCAGAAGG TCCCCGCGGT 
GTCGTCCTGG CCCTGCCCAC ACAGGGAGAG CCCATGGCCA CCAGCAGCAG CGAGCACGGC 
TCTGTCTCCC CAGACTCCGA CGCCCAGCCT GACGCACTCT TTGAGAGGAC CCGGGCGCCC 
AGGCACCACC TTGGCCACCT CCTCAGTGTT CCAGACGAGA GCTCAGCCTT GGAAGACCGG 
GGCTTGGCCT CGTCCCCGGA GGACAGGGAC CAGGGCCTCT TCCTGCTACG CAAGGACAGT 
GAGCGCCGTG CCATCCTGTA CAAAATCCTC TGGGAGGAGC AGAACCAGGT GGCTTCCAAC 
CTGCAGGAGT GTGTGGCCCA GAGTTCCGAA GAGTTGCATC TCTCAGTTGG ACACATCAAG 
CAAATCATTG GGATCCTGAG GGACTTCATC CGCTCCCCAG AGCACCGGGT GATGGCGACC 
ACAATATCAA AGCTCAAGGT GGACCTGGAC TTTGACAGCT CGTCCATCAG TCAGATTCAC 
CTGGTGCTGT TCGGATTTCA GGATGCCGTA AATAAAATTT TGAGGAACCA CTTAATTAGG 
CCCCACTGGA TGTTCGCGAT GGACAACATC ATCCGCCGAG CGGTGCAGGC CGCGGTCACC 
ATTCTCATCC CAGAGCTCCG AGCCCACTTT GAGCCTACCT GTGAGACTGA AGGGGTAGAT 
AAGGACATGG ATGAAGCGGA AGAGGGCTAT CCCCCAGCCA CCGGACCTGG CCAGGAGGCC 
CAGCCCCACC AGCAGCACCT GAGCCTCCAG CTGGGTGAGC TCAGACAGGA GACCAACAGA 
CTTTTGGAAC ACCTAGTTGA AAAAGAGAGA GAGTACCAGA ATCTTCTGCG GCAAACTCTA 
GAACAGAAAA CTCAAGAATT GTATCACCTT CAGTTAAAAT TAAAATCGAA TTGTATTACA 
GAGAACCCAG CAGGCCCCTA CGGGCAGAGA ACAGATAAAG AGCTTATAGA CTGGTTGCGG 
CTGCAAGGAG CTGATGCAAA GACAATTGAA AAGATTGTTG AAGAGGGTTA TACACTTTCG 
GATATTCTTA ATGAGATCAC TAAGGAAGAT CTAAGATACC TTCGACTACG GGGTGGTCTC 
CTCTGCAGAC TCTGGAGTGC GGTCTCCCAG TACAGAAGGG CTCAGGAGGC CTCAGAAACC 
AAAGACAAGG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K15 ( XM_011545508.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011543810.2 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu99305
Clone ID Related Accession (Same CDS sequence)	XM_011545508.2
Accession Version	XM_011545508.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3888bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545508.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGAAGT CTTTGACCAT 
AACATCAACC AATTTCAGGC ATCCAGTGGA AATTATTATT TCATCCCATA CATCGTGACA 
CCGTGCGCTG ATTATTTTTG CTGCGAGAGT GATGCCCAGA GACGAGCCTC CGAGTACATG 
CAGCCCAACT GGGACAACAT CCTGGGCCCG CTGTGCATGC CTTTGGTGGA CAGGTTCATT 
AGCCTCCTTA AGGACATCCA CGTGACCTCA TGTGTTTATT ACAAAGAAAC CTTGTTAAAT 
GACATCCGGA AAGCCAGAGA GAAATACCAA GGTGAGGAAC TGGCGAAGGA GCTAGCTCGG 
ATCAAGCTCC GCATGGATAA TACTGAGGTT CTGACCTCAG ACATCATCAT TAACTTACTC 
CTGTCCTACC GTGATATCCA GGACTATGAT GCGATGGTGA AGCTGGTGGA AACACTGGAG 
ATGCTGCCTA CGTGTGATTT GGCCGATCAG CATAACATTA AATTCCACTA TGCGTTTGCA 
CTGAATAGGA GAAACAGCAC AGGTGACCGT GAGAAGGCTC TGCAGATCAT GCTCCAGGTT 
CTGCAGAGCT GTGATCACCC GGGCCCCGAC ATGTTCTGCC TGTGTGGGAG GATCTACAAG 
GACATCTTCT TGGATTCAGA CTGCAAAGAT GACACCAGCC GCGACAGCGC CATTGAGTGG 
TATCGCAAAG GGTTTGAACT CCAGTCATCC CTCTATTCGG GAATTAATCT TGCAGTTTTG 
CTGATTGTTG CTGGACAACA ATTTGAAACT TCCTTGGAAC TAAGGAAAAT AGGTGTCCGG 
CTGAACAGTT TGTTGGGAAG AAAAGGGAGC TTGGAGAAAA TGAACAATTA CTGGGATGTG 
GGTCAGTTCT TCAGCGTCAG CATGCTGGCC CATGATGTCG GGAAAGCCGT CCAGGCAGCA 
GAGAGGTTGT TCAAACTGAA ACCTCCAGTC TGGTACCTGC GATCATTAGT TCAGAACTTG 
TTACTAATTC GGCGCTTCAA GAAAACCATT ATTGAACACT CGCCCAGGCA AGAGCGGCTG 
AACTTCTGGT TAGATATAAT TTTTGAGGCA ACAAATGAAG TCACTAATGG ACTCAGATTT 
CCAGTTCTGG TCATAGAGCC AACCAAAGTG TACCAGCCTT CTTATGTTTC CATAAACAAT 
GAAGCCGAGG AGAGAACAGT TTCTTTATGG CATGTCTCAC CCACAGAAAT GAAACAGATG 
CACGAATGGA ATTTTACAGC CTCTTCCATA AAGGGAATAA GCCTATCAAA GTTTGATGAA 
AGGTGTTGTT TTCTTTATGT CCATGATAAT TCTGATGACT TTCAAATCTA CTTTTCCACC 
GAAGAGCAGT GCAGTAGATT TTTCTCTTTG GTCAAAGAGA TGATAACCAA TACAGCAGGC 
AGTACGGTGG AGCTGGAGGG AGAGACCGAT GGAGACACCT TGGAGTATGA GTATGACCAT 
GATGCAAATG GTGAGAGAGT TGTCTTGGGG AAAGGCACGT ATGGGATTGT GTATGCTGGC 
CGAGATCTGA GCAATCAAGT GCGAATAGCC ATCAAAGAAA TCCCGGAGAG AGATAGCAGG 
TATTCTCAGC CTCTGCACGA GGAGATAGCC CTGCACAAGT ACCTTAAGCA CCGCAATATC 
GTTCAGTACC TGGGCTCTGT TTCAGAGAAC GGCTACATTA AGATATTTAT GGAGCAGGTG 
CCTGGAGGAA GCCTTTCTGC TCTTCTGCGA TCCAAATGGG GGCCGATGAA GGAACCGACA 
ATCAAGTTTT ACACCAAACA GATCCTGGAG GGCCTTAAGT ATCTTCATGA AAACCAGATC 
GTGCACAGAG ACATAAAGGG CGATAATGTT CTGGTGAACA CCTACAGCGG AGTGGTGAAA 
ATCTCCGATT TTGGAACCTC GAAACGTCTT GCGGGTGTGA ACCCCTGCAC AGAGACTTTT 
ACTGGCACCC TGCAGTACAT GGCACCTGAG ATAATTGACC AAGGGCCTCG CGGATATGGT 
GCCCCAGCCG ATATCTGGTC CCTGGGCTGC ACCATCATTG AGATGGCCAC CAGCAAGCCT 
CCGTTCCATG AGCTTGGTGA GCCGCAGGCA GCCATGTTCA AAGTGGGCAT GTTTAAGATC 
CACCCTGAGA TTCCAGAAGC CCTTTCAGCT GAAGCCCGAG CCTTCATTTT ATCCTGTTTC 
GAGCCTGACC CCCACAAACG TGCCACCACT GCTGAGCTAC TGAGAGAGGG TTTCTTAAGG 
CAGGTGAACA AGGGCAAGAA GAACCGAATT GCCTTCAAGC CCTCAGACTC CGACGCCCAG 
CCTGACGCAC TCTTTGAGAG GACCCGGGCG CCCAGGCACC ACCTTGGCCA CCTCCTCAGT 
GTTCCAGACG AGAGCTCAGC CTTGGAAGAC CGGGGCTTGG CCTCGTCCCC GGAGGACAGG 
GACCAGGGCC TCTTCCTGCT ACGCAAGGAC AGTGAGCGCC GTGCCATCCT GTACAAAATC 
CTCTGGGAGG AGCAGAACCA GGTGGCTTCC AACCTGCAGG AGTGTGTGGC CCAGAGTTCC 
GAAGAGTTGC ATCTCTCAGT TGGACACATC AAGCAAATCA TTGGGATCCT GAGGGACTTC 
ATCCGCTCCC CAGAGCACCG GGTGATGGCG ACCACAATAT CAAAGCTCAA GGTGGACCTG 
GACTTTGACA GCTCGTCCAT CAGTCAGATT CACCTGGTGC TGTTCGGATT TCAGGATGCC 
GTAAATAAAA TTTTGAGGAA CCACTTAATT AGGCCCCACT GGATGTTCGC GATGGACAAC 
ATCATCCGCC GAGCGGTGCA GGCCGCGGTC ACCATTCTCA TCCCAGAGCT CCGAGCCCAC 
TTTGAGCCTA CCTGTGAGAC TGAAGGGGTA GATAAGGACA TGGATGAAGC GGAAGAGGGC 
TATCCCCCAG CCACCGGACC TGGCCAGGAG GCCCAGCCCC ACCAGCAGCA CCTGAGCCTC 
CAGCTGGGTG AGCTCAGACA GGAGACCAAC AGACTTTTGG AACACCTAGT TGAAAAAGAG 
AGAGAGTACC AGAATCTTCT GCGGCAAACT CTAGAACAGA AAACTCAAGA ATTGTATCAC 
CTTCAGTTAA AATTAAAATC GAATTGTATT ACAGAGAACC CAGCAGGCCC CTACGGGCAG 
AGAACAGATA AAGAGCTTAT AGACTGGTTG CGGCTGCAAG GAGCTGATGC AAAGACAATT 
GAAAAGATTG TTGAAGAGGG TTATACACTT TCGGATATTC TTAATGAGAT CACTAAGGAA 
GATCTAAGAT ACCTTCGACT ACGGGGTGGT CTCCTCTGCA GACTCTGGAG TGCGGTCTCC 
CAGTACAGAA GGGCTCAGGA GGCCTCAGAA ACCAAAGACA AGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011543810.2
CDS	195..4082
Translation	MESGGGNAPAGALGAASESPQCPPPPGVEGAAGPAEPDGAAEGAAGGSGEGESGGGPRRALRAVYVRSESSQGGAAGGPEAGARQCLLRACEAEGAHLTSVPFGELDFGETAVLDAFYDADVAVVDMSDVSRQPSLFYHLGVRESFDMANNVILYHDTDADTALSLKDMVTQKNTEVFDHNINQFQASSGNYYFIPYIVTPCADYFCCESDAQRRASEYMQPNWDNILGPLCMPLVDRFISLLKDIHVTSCVYYKETLLNDIRKAREKYQGEELAKELARIKLRMDNTEVLTSDIIINLLLSYRDIQDYDAMVKLVETLEMLPTCDLADQHNIKFHYAFALNRRNSTGDREKALQIMLQVLQSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRKGFELQSSLYSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVGQFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGTLQYMAPEIIDQGPRGYGAPADIWSLGCTIIEMATSKPPFHELGEPQAAMFKVGMFKIHPEIPEALSAEARAFILSCFEPDPHKRATTAELLREGFLRQVNKGKKNRIAFKPSDSDAQPDALFERTRAPRHHLGHLLSVPDESSALEDRGLASSPEDRDQGLFLLRKDSERRAILYKILWEEQNQVASNLQECVAQSSEELHLSVGHIKQIIGILRDFIRSPEHRVMATTISKLKVDLDFDSSSISQIHLVLFGFQDAVNKILRNHLIRPHWMFAMDNIIRRAVQAAVTILIPELRAHFEPTCETEGVDKDMDEAEEGYPPATGPGQEAQPHQQHLSLQLGELRQETNRLLEHLVEKEREYQNLLRQTLEQKTQELYHLQLKLKSNCITENPAGPYGQRTDKELIDWLRLQGADAKTIEKIVEEGYTLSDILNEITKEDLRYLRLRGGLLCRLWSAVSQYRRAQEASETKDKA

Target ORF information:

RefSeq Version	XM_011545508.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545508.2

ORF Insert Sequence:

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGAAGT CTTTGACCAT 
AACATCAACC AATTTCAGGC ATCCAGTGGA AATTATTATT TCATCCCATA CATCGTGACA 
CCGTGCGCTG ATTATTTTTG CTGCGAGAGT GATGCCCAGA GACGAGCCTC CGAGTACATG 
CAGCCCAACT GGGACAACAT CCTGGGCCCG CTGTGCATGC CTTTGGTGGA CAGGTTCATT 
AGCCTCCTTA AGGACATCCA CGTGACCTCA TGTGTTTATT ACAAAGAAAC CTTGTTAAAT 
GACATCCGGA AAGCCAGAGA GAAATACCAA GGTGAGGAAC TGGCGAAGGA GCTAGCTCGG 
ATCAAGCTCC GCATGGATAA TACTGAGGTT CTGACCTCAG ACATCATCAT TAACTTACTC 
CTGTCCTACC GTGATATCCA GGACTATGAT GCGATGGTGA AGCTGGTGGA AACACTGGAG 
ATGCTGCCTA CGTGTGATTT GGCCGATCAG CATAACATTA AATTCCACTA TGCGTTTGCA 
CTGAATAGGA GAAACAGCAC AGGTGACCGT GAGAAGGCTC TGCAGATCAT GCTCCAGGTT 
CTGCAGAGCT GTGATCACCC GGGCCCCGAC ATGTTCTGCC TGTGTGGGAG GATCTACAAG 
GACATCTTCT TGGATTCAGA CTGCAAAGAT GACACCAGCC GCGACAGCGC CATTGAGTGG 
TATCGCAAAG GGTTTGAACT CCAGTCATCC CTCTATTCGG GAATTAATCT TGCAGTTTTG 
CTGATTGTTG CTGGACAACA ATTTGAAACT TCCTTGGAAC TAAGGAAAAT AGGTGTCCGG 
CTGAACAGTT TGTTGGGAAG AAAAGGGAGC TTGGAGAAAA TGAACAATTA CTGGGATGTG 
GGTCAGTTCT TCAGCGTCAG CATGCTGGCC CATGATGTCG GGAAAGCCGT CCAGGCAGCA 
GAGAGGTTGT TCAAACTGAA ACCTCCAGTC TGGTACCTGC GATCATTAGT TCAGAACTTG 
TTACTAATTC GGCGCTTCAA GAAAACCATT ATTGAACACT CGCCCAGGCA AGAGCGGCTG 
AACTTCTGGT TAGATATAAT TTTTGAGGCA ACAAATGAAG TCACTAATGG ACTCAGATTT 
CCAGTTCTGG TCATAGAGCC AACCAAAGTG TACCAGCCTT CTTATGTTTC CATAAACAAT 
GAAGCCGAGG AGAGAACAGT TTCTTTATGG CATGTCTCAC CCACAGAAAT GAAACAGATG 
CACGAATGGA ATTTTACAGC CTCTTCCATA AAGGGAATAA GCCTATCAAA GTTTGATGAA 
AGGTGTTGTT TTCTTTATGT CCATGATAAT TCTGATGACT TTCAAATCTA CTTTTCCACC 
GAAGAGCAGT GCAGTAGATT TTTCTCTTTG GTCAAAGAGA TGATAACCAA TACAGCAGGC 
AGTACGGTGG AGCTGGAGGG AGAGACCGAT GGAGACACCT TGGAGTATGA GTATGACCAT 
GATGCAAATG GTGAGAGAGT TGTCTTGGGG AAAGGCACGT ATGGGATTGT GTATGCTGGC 
CGAGATCTGA GCAATCAAGT GCGAATAGCC ATCAAAGAAA TCCCGGAGAG AGATAGCAGG 
TATTCTCAGC CTCTGCACGA GGAGATAGCC CTGCACAAGT ACCTTAAGCA CCGCAATATC 
GTTCAGTACC TGGGCTCTGT TTCAGAGAAC GGCTACATTA AGATATTTAT GGAGCAGGTG 
CCTGGAGGAA GCCTTTCTGC TCTTCTGCGA TCCAAATGGG GGCCGATGAA GGAACCGACA 
ATCAAGTTTT ACACCAAACA GATCCTGGAG GGCCTTAAGT ATCTTCATGA AAACCAGATC 
GTGCACAGAG ACATAAAGGG CGATAATGTT CTGGTGAACA CCTACAGCGG AGTGGTGAAA 
ATCTCCGATT TTGGAACCTC GAAACGTCTT GCGGGTGTGA ACCCCTGCAC AGAGACTTTT 
ACTGGCACCC TGCAGTACAT GGCACCTGAG ATAATTGACC AAGGGCCTCG CGGATATGGT 
GCCCCAGCCG ATATCTGGTC CCTGGGCTGC ACCATCATTG AGATGGCCAC CAGCAAGCCT 
CCGTTCCATG AGCTTGGTGA GCCGCAGGCA GCCATGTTCA AAGTGGGCAT GTTTAAGATC 
CACCCTGAGA TTCCAGAAGC CCTTTCAGCT GAAGCCCGAG CCTTCATTTT ATCCTGTTTC 
GAGCCTGACC CCCACAAACG TGCCACCACT GCTGAGCTAC TGAGAGAGGG TTTCTTAAGG 
CAGGTGAACA AGGGCAAGAA GAACCGAATT GCCTTCAAGC CCTCAGACTC CGACGCCCAG 
CCTGACGCAC TCTTTGAGAG GACCCGGGCG CCCAGGCACC ACCTTGGCCA CCTCCTCAGT 
GTTCCAGACG AGAGCTCAGC CTTGGAAGAC CGGGGCTTGG CCTCGTCCCC GGAGGACAGG 
GACCAGGGCC TCTTCCTGCT ACGCAAGGAC AGTGAGCGCC GTGCCATCCT GTACAAAATC 
CTCTGGGAGG AGCAGAACCA GGTGGCTTCC AACCTGCAGG AGTGTGTGGC CCAGAGTTCC 
GAAGAGTTGC ATCTCTCAGT TGGACACATC AAGCAAATCA TTGGGATCCT GAGGGACTTC 
ATCCGCTCCC CAGAGCACCG GGTGATGGCG ACCACAATAT CAAAGCTCAA GGTGGACCTG 
GACTTTGACA GCTCGTCCAT CAGTCAGATT CACCTGGTGC TGTTCGGATT TCAGGATGCC 
GTAAATAAAA TTTTGAGGAA CCACTTAATT AGGCCCCACT GGATGTTCGC GATGGACAAC 
ATCATCCGCC GAGCGGTGCA GGCCGCGGTC ACCATTCTCA TCCCAGAGCT CCGAGCCCAC 
TTTGAGCCTA CCTGTGAGAC TGAAGGGGTA GATAAGGACA TGGATGAAGC GGAAGAGGGC 
TATCCCCCAG CCACCGGACC TGGCCAGGAG GCCCAGCCCC ACCAGCAGCA CCTGAGCCTC 
CAGCTGGGTG AGCTCAGACA GGAGACCAAC AGACTTTTGG AACACCTAGT TGAAAAAGAG 
AGAGAGTACC AGAATCTTCT GCGGCAAACT CTAGAACAGA AAACTCAAGA ATTGTATCAC 
CTTCAGTTAA AATTAAAATC GAATTGTATT ACAGAGAACC CAGCAGGCCC CTACGGGCAG 
AGAACAGATA AAGAGCTTAT AGACTGGTTG CGGCTGCAAG GAGCTGATGC AAAGACAATT 
GAAAAGATTG TTGAAGAGGG TTATACACTT TCGGATATTC TTAATGAGAT CACTAAGGAA 
GATCTAAGAT ACCTTCGACT ACGGGGTGGT CTCCTCTGCA GACTCTGGAG TGCGGTCTCC 
CAGTACAGAA GGGCTCAGGA GGCCTCAGAA ACCAAAGACA AGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAP3K15 ( XM_017029511.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016885000.1 Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X4, mRNA.

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CloneID	OHu99306
Clone ID Related Accession (Same CDS sequence)	XM_017029511.1
Accession Version	XM_017029511.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	mitogen-activated protein kinase kinase kinase 15 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGAAGT CTTTGACCAT 
AACATCAACC AATTTCAGGC ATCCAGTGGA AATTATTATT TCATCCCATA CATCGTGACA 
CCGTGCGCTG ATTATTTTTG CTGCGAGAGT GATGCCCAGA GACGAGCCTC CGAGTACATG 
CAGCCCAACT GGGACAACAT CCTGGGCCCG CTGTGCATGC CTTTGGTGGA CAGGTTCATT 
AGCCTCCTTA AGGACATCCA CGTGACCTCA TGTGTTTATT ACAAAGAAAC CTTGTTAAAT 
GACATCCGGA AAGCCAGAGA GAAATACCAA GGTGAGGAAC TGGCGAAGGA GCTAGCTCGG 
ATCAAGCTCC GCATGGATAA TACTGAGGTT CTGACCTCAG ACATCATCAT TAACTTACTC 
CTGTCCTACC GTGATATCCA GGACTATGAT GCGATGGTGA AGCTGGTGGA AACACTGGAG 
ATGCTGCCTA CGTGTGATTT GGCCGATCAG CATAACATTA AATTCCACTA TGCGTTTGCA 
CTGAATAGGA GAAACAGCAC AGGTGACCGT GAGAAGGCTC TGCAGATCAT GCTCCAGGTT 
CTGCAGAGCT GTGATCACCC GGGCCCCGAC ATGTTCTGCC TGTGTGGGAG GATCTACAAG 
GACATCTTCT TGGATTCAGA CTGCAAAGAT GACACCAGCC GCGACAGCGC CATTGAGTGG 
TATCGCAAAG GGTTTGAACT CCAGTCATCC CTCTATTCGG GAATTAATCT TGCAGTTTTG 
CTGATTGTTG CTGGACAACA ATTTGAAACT TCCTTGGAAC TAAGGAAAAT AGGTGTCCGG 
CTGAACAGTT TGTTGGGAAG AAAAGGGAGC TTGGAGAAAA TGAACAATTA CTGGGATGTG 
GGTCAGTTCT TCAGCGTCAG CATGCTGGCC CATGATGTCG GGAAAGCCGT CCAGGCAGCA 
GAGAGGTTGT TCAAACTGAA ACCTCCAGTC TGGTACCTGC GATCATTAGT TCAGAACTTG 
TTACTAATTC GGCGCTTCAA GAAAACCATT ATTGAACACT CGCCCAGGCA AGAGCGGCTG 
AACTTCTGGT TAGATATAAT TTTTGAGGCA ACAAATGAAG TCACTAATGG ACTCAGATTT 
CCAGTTCTGG TCATAGAGCC AACCAAAGTG TACCAGCCTT CTTATGTTTC CATAAACAAT 
GAAGCCGAGG AGAGAACAGT TTCTTTATGG CATGTCTCAC CCACAGAAAT GAAACAGATG 
CACGAATGGA ATTTTACAGC CTCTTCCATA AAGGGAATAA GCCTATCAAA GTTTGATGAA 
AGGTGTTGTT TTCTTTATGT CCATGATAAT TCTGATGACT TTCAAATCTA CTTTTCCACC 
GAAGAGCAGT GCAGTAGATT TTTCTCTTTG GTCAAAGAGA TGATAACCAA TACAGCAGGC 
AGTACGGTGG AGCTGGAGGG AGAGACCGAT GGAGACACCT TGGAGTATGA GTATGACCAT 
GATGCAAATG GTGAGAGAGT TGTCTTGGGG AAAGGCACGT ATGGGATTGT GTATGCTGGC 
CGAGATCTGA GCAATCAAGT GCGAATAGCC ATCAAAGAAA TCCCGGAGAG AGATAGCAGG 
TATTCTCAGC CTCTGCACGA GGAGATAGCC CTGCACAAGT ACCTTAAGCA CCGCAATATC 
GTTCAGTACC TGGGCTCTGT TTCAGAGAAC GGCTACATTA AGATATTTAT GGAGCAGGTG 
CCTGGAGGAA GCCTTTCTGC TCTTCTGCGA TCCAAATGGG GGCCGATGAA GGAACCGACA 
ATCAAGTTTT ACACCAAACA GATCCTGGAG GGCCTTAAGT ATCTTCATGA AAACCAGATC 
GTGCACAGAG ACATAAAGGG CGATAATGTT CTGGTGAACA CCTACAGCGG AGTGGTGAAA 
ATCTCCGATT TTGGAACCTC GAAACGTCTT GCGGGTGTGA ACCCCTGCAC AGAGACTTTT 
ACTGGTGGGC ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016885000.1
CDS	197..2674
Translation	MESGGGNAPAGALGAASESPQCPPPPGVEGAAGPAEPDGAAEGAAGGSGEGESGGGPRRALRAVYVRSESSQGGAAGGPEAGARQCLLRACEAEGAHLTSVPFGELDFGETAVLDAFYDADVAVVDMSDVSRQPSLFYHLGVRESFDMANNVILYHDTDADTALSLKDMVTQKNTEVFDHNINQFQASSGNYYFIPYIVTPCADYFCCESDAQRRASEYMQPNWDNILGPLCMPLVDRFISLLKDIHVTSCVYYKETLLNDIRKAREKYQGEELAKELARIKLRMDNTEVLTSDIIINLLLSYRDIQDYDAMVKLVETLEMLPTCDLADQHNIKFHYAFALNRRNSTGDREKALQIMLQVLQSCDHPGPDMFCLCGRIYKDIFLDSDCKDDTSRDSAIEWYRKGFELQSSLYSGINLAVLLIVAGQQFETSLELRKIGVRLNSLLGRKGSLEKMNNYWDVGQFFSVSMLAHDVGKAVQAAERLFKLKPPVWYLRSLVQNLLLIRRFKKTIIEHSPRQERLNFWLDIIFEATNEVTNGLRFPVLVIEPTKVYQPSYVSINNEAEERTVSLWHVSPTEMKQMHEWNFTASSIKGISLSKFDERCCFLYVHDNSDDFQIYFSTEEQCSRFFSLVKEMITNTAGSTVELEGETDGDTLEYEYDHDANGERVVLGKGTYGIVYAGRDLSNQVRIAIKEIPERDSRYSQPLHEEIALHKYLKHRNIVQYLGSVSENGYIKIFMEQVPGGSLSALLRSKWGPMKEPTIKFYTKQILEGLKYLHENQIVHRDIKGDNVLVNTYSGVVKISDFGTSKRLAGVNPCTETFTGGHV

Target ORF information:

RefSeq Version	XM_017029511.1
Organism	Homo sapiens(human)
Definition	Homo sapiens mitogen-activated protein kinase kinase kinase 15 (MAP3K15), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017029511.1

ORF Insert Sequence:

ATGGAGAGCG GCGGTGGGAA TGCTCCGGCC GGGGCCCTCG GGGCGGCGAG CGAGTCCCCT 
CAGTGCCCGC CGCCGCCGGG GGTGGAGGGC GCGGCCGGGC CGGCGGAGCC CGACGGGGCG 
GCGGAGGGCG CGGCAGGCGG CAGCGGCGAG GGCGAGAGTG GGGGCGGGCC GCGGCGGGCT 
CTGCGGGCAG TATACGTGCG CAGTGAGAGC TCCCAGGGCG GCGCGGCCGG CGGCCCGGAG 
GCTGGGGCGC GGCAGTGCCT GCTGCGGGCC TGCGAGGCCG AGGGCGCTCA CCTCACCTCC 
GTGCCCTTCG GGGAGCTGGA CTTCGGGGAG ACGGCCGTGC TCGACGCCTT CTACGACGCA 
GATGTTGCTG TGGTAGACAT GAGCGATGTC TCCAGACAGC CTTCCCTCTT CTACCATCTT 
GGAGTCCGAG AAAGCTTTGA CATGGCCAAT AATGTGATCT TGTACCATGA CACCGATGCC 
GACACTGCTC TCTCTTTGAA GGACATGGTA ACTCAAAAAA ACACAGAAGT CTTTGACCAT 
AACATCAACC AATTTCAGGC ATCCAGTGGA AATTATTATT TCATCCCATA CATCGTGACA 
CCGTGCGCTG ATTATTTTTG CTGCGAGAGT GATGCCCAGA GACGAGCCTC CGAGTACATG 
CAGCCCAACT GGGACAACAT CCTGGGCCCG CTGTGCATGC CTTTGGTGGA CAGGTTCATT 
AGCCTCCTTA AGGACATCCA CGTGACCTCA TGTGTTTATT ACAAAGAAAC CTTGTTAAAT 
GACATCCGGA AAGCCAGAGA GAAATACCAA GGTGAGGAAC TGGCGAAGGA GCTAGCTCGG 
ATCAAGCTCC GCATGGATAA TACTGAGGTT CTGACCTCAG ACATCATCAT TAACTTACTC 
CTGTCCTACC GTGATATCCA GGACTATGAT GCGATGGTGA AGCTGGTGGA AACACTGGAG 
ATGCTGCCTA CGTGTGATTT GGCCGATCAG CATAACATTA AATTCCACTA TGCGTTTGCA 
CTGAATAGGA GAAACAGCAC AGGTGACCGT GAGAAGGCTC TGCAGATCAT GCTCCAGGTT 
CTGCAGAGCT GTGATCACCC GGGCCCCGAC ATGTTCTGCC TGTGTGGGAG GATCTACAAG 
GACATCTTCT TGGATTCAGA CTGCAAAGAT GACACCAGCC GCGACAGCGC CATTGAGTGG 
TATCGCAAAG GGTTTGAACT CCAGTCATCC CTCTATTCGG GAATTAATCT TGCAGTTTTG 
CTGATTGTTG CTGGACAACA ATTTGAAACT TCCTTGGAAC TAAGGAAAAT AGGTGTCCGG 
CTGAACAGTT TGTTGGGAAG AAAAGGGAGC TTGGAGAAAA TGAACAATTA CTGGGATGTG 
GGTCAGTTCT TCAGCGTCAG CATGCTGGCC CATGATGTCG GGAAAGCCGT CCAGGCAGCA 
GAGAGGTTGT TCAAACTGAA ACCTCCAGTC TGGTACCTGC GATCATTAGT TCAGAACTTG 
TTACTAATTC GGCGCTTCAA GAAAACCATT ATTGAACACT CGCCCAGGCA AGAGCGGCTG 
AACTTCTGGT TAGATATAAT TTTTGAGGCA ACAAATGAAG TCACTAATGG ACTCAGATTT 
CCAGTTCTGG TCATAGAGCC AACCAAAGTG TACCAGCCTT CTTATGTTTC CATAAACAAT 
GAAGCCGAGG AGAGAACAGT TTCTTTATGG CATGTCTCAC CCACAGAAAT GAAACAGATG 
CACGAATGGA ATTTTACAGC CTCTTCCATA AAGGGAATAA GCCTATCAAA GTTTGATGAA 
AGGTGTTGTT TTCTTTATGT CCATGATAAT TCTGATGACT TTCAAATCTA CTTTTCCACC 
GAAGAGCAGT GCAGTAGATT TTTCTCTTTG GTCAAAGAGA TGATAACCAA TACAGCAGGC 
AGTACGGTGG AGCTGGAGGG AGAGACCGAT GGAGACACCT TGGAGTATGA GTATGACCAT 
GATGCAAATG GTGAGAGAGT TGTCTTGGGG AAAGGCACGT ATGGGATTGT GTATGCTGGC 
CGAGATCTGA GCAATCAAGT GCGAATAGCC ATCAAAGAAA TCCCGGAGAG AGATAGCAGG 
TATTCTCAGC CTCTGCACGA GGAGATAGCC CTGCACAAGT ACCTTAAGCA CCGCAATATC 
GTTCAGTACC TGGGCTCTGT TTCAGAGAAC GGCTACATTA AGATATTTAT GGAGCAGGTG 
CCTGGAGGAA GCCTTTCTGC TCTTCTGCGA TCCAAATGGG GGCCGATGAA GGAACCGACA 
ATCAAGTTTT ACACCAAACA GATCCTGGAG GGCCTTAAGT ATCTTCATGA AAACCAGATC 
GTGCACAGAG ACATAAAGGG CGATAATGTT CTGGTGAACA CCTACAGCGG AGTGGTGAAA 
ATCTCCGATT TTGGAACCTC GAAACGTCTT GCGGGTGTGA ACCCCTGCAC AGAGACTTTT 
ACTGGTGGGC ATGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ASK3 responds to osmotic stress and regulates blood pressure by suppressing WNK1-SPAK/OSR1 signaling in the kidney.
Nature communications31285(2012)
Naguro I,Umeda T,Kobayashi Y,Maruyama J,Hattori K,Shimizu Y,Kataoka K,Kim-Mitsuyama S,Uchida S,Vandewalle A,Noguchi T,Nishitoh H,Matsuzawa A,Takeda K,Ichijo H

ASK3, a novel member of the apoptosis signal-regulating kinase family, is essential for stress-induced cell death in HeLa cells.
Biochemical and biophysical research communications395(2)213-8(2010 Apr)
Kaji T,Yoshida S,Kawai K,Fuchigami Y,Watanabe W,Kubodera H,Kishimoto T

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MAP3K15 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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