MAFA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAFA
Entrez Gene ID	389692
Full Name	MAF bZIP transcription factor A
Synonyms	RIPE3b1,hMafA
General protein information	Preferred Names MAF bZIP transcription factor A Names transcription factor MafA pancreatic beta-cell-specific transcriptional activator transcription factor RIPE3b1 v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog A
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 8 Map Location: 8q24.3
Summary	MAFA is a transcription factor that binds RIPE3b, a conserved enhancer element that regulates pancreatic beta cell-specific expression of the insulin gene (INS; MIM 176730) (Olbrot et al., 2002 [PubMed 12011435]).[supplied by OMIM, Mar 2008].
Disorder MIM:	610303

mRNA and Protein(s)

mRNA	Protein	Name
NM_201589.3	NP_963883.2	transcription factor MafA
NM_201589.4	NP_963883.2	transcription factor MafA

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Mafa	NP_919331.1
Gallus gallus (chicken)	MAFA	NP_990356.1
Danio rerio (zebrafish)	mafa	NP_001076409.1
Bos taurus (cattle)	MAFA	XP_005215337.1
Canis lupus familiaris (dog)	MAFA	XP_003431862.2
Rattus norvegicus (Norway rat)	Mafa	XP_006241965.1
Homo sapiens (human)	MAFA	NP_963883.2
Xenopus tropicalis (tropical clawed frog)	mafa	NP_001027475.1

Gene Ontology
Bibliography

Related articles in PubMed

Iacovazzo D, Flanagan SE, Walker E, Quezado R, de Sousa Barros FA, Caswell R, Johnson MB, Wakeling M, Br?ndle M, Guo M, Dang MN, Gabrovska P, Niederle B, Christ E, Jenni S, Sipos B, Nieser M, Frilling A, Dhatariya K, Chanson P, de Herder WW, Konukiewitz B, Kl?ppel G, Stein R, Korbonits M, Ellard S
Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)

PDX1, Neurogenin-3, and MAFA: critical transcription regulators for beta cell development and regeneration.
Zhu Y, Liu Q, Zhou Z, Ikeda Y
Stem cell research & therapy8(1)240(2017 Nov)

miR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA.
Marzinotto I, Pellegrini S, Brigatti C, Nano R, Melzi R, Mercalli A, Liberati D, Sordi V, Ferrari M, Falconi M, Doglioni C, Ravassard P, Piemonti L, Lampasona V
Scientific reports7(1)14051(2017 Oct)

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis.
Wang S, Juan J, Zhang Z, Du Y, Xu Y, Tong J, Cao B, Moran MF, Zeng Y, Mao X
Cell death & disease8(9)e3058(2017 Sep)

MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes.
Ganic E, Singh T, Luan C, Fadista J, Johansson JK, Cyphert HA, Bennet H, Storm P, Prost G, Ahlenius H, Renstr?m E, Stein R, Groop L, Fex M, Artner I
Cell reports14(8)1991-2002(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pdx1 and MafA play crucial roles in the pancreas and maintain mature beta-cell function. Our results showed that the expression of Pdx1 and MafA were significantly upregulated after a sleeve gastrectomy for morbid obesity.
Title: Upregulated Pdx1 and MafA Contribute to ?-Cell Function Improvement by Sleeve Gastrectomy.

MAFA controls autonomic nervous system-mediated insulin secretion by activating the transcription of nicotinic (ChrnB2 and ChrnB4) receptor genes, which is impaired in patients with type 2 diabetes.
Title: MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes.

These findings demonstrate that regulation of monoamine levels by Mao activity in beta cells is pivotal for physiological insulin secretion and that loss of MaoB expression may contribute to the beta cell dysfunction in type 2 diabetes.
Title: Islet-specific monoamine oxidase A and B expression depends on MafA transcriptional activity and is compromised in type 2 diabetes.

Loss of MAFA expression is associated with insulinoma.
Title: Altered MENIN expression disrupts the MAFA differentiation pathway in insulinoma.

MAFA nuclear expression in pancreatic alpha and beta cells, and the percentage of alpha cells expressing PAX4 are altered in patients with type 2 diabetes.
Title: Both PAX4 and MAFA are expressed in a substantial proportion of normal human pancreatic alpha cells and deregulated in patients with type 2 diabetes.

The following MAFA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAFA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23130	NM_201589.4 Latest version!	Homo sapiens MAF bZIP transcription factor A (MAFA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu23130	NM_201589.3	Homo sapiens MAF bZIP transcription factor A (MAFA), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAFA ( NM_201589.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_963883.2 Homo sapiens MAF bZIP transcription factor A (MAFA), mRNA.

Price & Availability↑

CloneID	OHu23130
Clone ID Related Accession (Same CDS sequence)	NM_201589.3 , NM_201589.4
Accession Version	NM_201589.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	transcription factor MafA
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105118.23. On Dec 3, 2010 this sequence version replaced NM_201589.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCCGCGG AGCTGGCGAT GGGCGCCGAG CTGCCCAGCA GCCCGCTGGC CATCGAGTAC 
GTCAACGACT TCGACCTGAT GAAGTTCGAG GTGAAGAAGG AGCCTCCCGA GGCCGAGCGC 
TTCTGCCACC GCCTGCCGCC AGGCTCGCTG TCCTCGACGC CGCTCAGCAC GCCCTGCTCC 
TCCGTGCCCT CCTCGCCCAG CTTCTGCGCG CCCAGCCCGG GCACCGGCGG CGGCGGCGGC 
GCGGGGGGCG GCGGCGGCTC GTCTCAGGCC GGGGGCGCCC CCGGGCCGCC GAGCGGGGGC 
CCCGGCGCCG TCGGGGGCAC CTCGGGGAAG CCGGCGCTGG AGGATCTGTA CTGGATGAGC 
GGCTACCAGC ATCACCTCAA CCCCGAGGCG CTCAACCTGA CGCCCGAGGA CGCGGTGGAG 
GCGCTCATCG GCAGCGGCCA CCACGGCGCG CACCACGGCG CGCACCACCC GGCGGCCGCC 
GCAGCCTACG AGGCTTTCCG CGGCCCGGGC TTCGCGGGCG GCGGCGGAGC GGACGACATG 
GGCGCCGGCC ACCACCACGG CGCGCACCAC GCCGCCCACC ATCACCACGC CGCCCACCAC 
CACCACCACC ACCACCACCA CCATGGCGGC GCGGGACACG GCGGTGGCGC GGGCCACCAC 
GTGCGCCTGG AGGAGCGCTT CTCCGACGAC CAGCTGGTGT CCATGTCGGT GCGCGAGCTG 
AACCGGCAGC TCCGCGGCTT CAGCAAGGAG GAGGTCATCC GGCTCAAGCA GAAGCGGCGC 
ACGCTCAAGA ACCGCGGCTA CGCGCAGTCC TGCCGCTTCA AGCGGGTGCA GCAGCGGCAC 
ATTCTGGAGA GCGAGAAGTG CCAACTCCAG AGCCAGGTGG AGCAGCTGAA GCTGGAGGTG 
GGGCGCCTGG CCAAAGAGCG GGACCTGTAC AAGGAGAAAT ACGAGAAGCT GGCGGGCCGG 
GGCGGCCCCG GGAGCGCGGG CGGGGCCGGT TTCCCGCGGG AGCCTTCGCC GCCGCAGGCC 
GGTCCCGGCG GGGCCAAGGG CACGGCCGAC TTCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963883.2
CDS	27..1088
Translation	MAAELAMGAELPSSPLAIEYVNDFDLMKFEVKKEPPEAERFCHRLPPGSLSSTPLSTPCSSVPSSPSFCAPSPGTGGGGGAGGGGGSSQAGGAPGPPSGGPGAVGGTSGKPALEDLYWMSGYQHHLNPEALNLTPEDAVEALIGSGHHGAHHGAHHPAAAAAYEAFRGPGFAGGGGADDMGAGHHHGAHHAAHHHHAAHHHHHHHHHHGGAGHGGGAGHHVRLEERFSDDQLVSMSVRELNRQLRGFSKEEVIRLKQKRRTLKNRGYAQSCRFKRVQQRHILESEKCQLQSQVEQLKLEVGRLAKERDLYKEKYEKLAGRGGPGSAGGAGFPREPSPPQAGPGGAKGTADFFL

Target ORF information:

RefSeq Version	NM_201589.3
Organism	Homo sapiens(human)
Definition	Homo sapiens MAF bZIP transcription factor A (MAFA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201589.3

ORF Insert Sequence:

ATGGCCGCGG AGCTGGCGAT GGGCGCCGAG CTGCCCAGCA GCCCGCTGGC CATCGAGTAC 
GTCAACGACT TCGACCTGAT GAAGTTCGAG GTGAAGAAGG AGCCTCCCGA GGCCGAGCGC 
TTCTGCCACC GCCTGCCGCC AGGCTCGCTG TCCTCGACGC CGCTCAGCAC GCCCTGCTCC 
TCCGTGCCCT CCTCGCCCAG CTTCTGCGCG CCCAGCCCGG GCACCGGCGG CGGCGGCGGC 
GCGGGGGGCG GCGGCGGCTC GTCTCAGGCC GGGGGCGCCC CCGGGCCGCC GAGCGGGGGC 
CCCGGCGCCG TCGGGGGCAC CTCGGGGAAG CCGGCGCTGG AGGATCTGTA CTGGATGAGC 
GGCTACCAGC ATCACCTCAA CCCCGAGGCG CTCAACCTGA CGCCCGAGGA CGCGGTGGAG 
GCGCTCATCG GCAGCGGCCA CCACGGCGCG CACCACGGCG CGCACCACCC GGCGGCCGCC 
GCAGCCTACG AGGCTTTCCG CGGCCCGGGC TTCGCGGGCG GCGGCGGAGC GGACGACATG 
GGCGCCGGCC ACCACCACGG CGCGCACCAC GCCGCCCACC ATCACCACGC CGCCCACCAC 
CACCACCACC ACCACCACCA CCATGGCGGC GCGGGACACG GCGGTGGCGC GGGCCACCAC 
GTGCGCCTGG AGGAGCGCTT CTCCGACGAC CAGCTGGTGT CCATGTCGGT GCGCGAGCTG 
AACCGGCAGC TCCGCGGCTT CAGCAAGGAG GAGGTCATCC GGCTCAAGCA GAAGCGGCGC 
ACGCTCAAGA ACCGCGGCTA CGCGCAGTCC TGCCGCTTCA AGCGGGTGCA GCAGCGGCAC 
ATTCTGGAGA GCGAGAAGTG CCAACTCCAG AGCCAGGTGG AGCAGCTGAA GCTGGAGGTG 
GGGCGCCTGG CCAAAGAGCG GGACCTGTAC AAGGAGAAAT ACGAGAAGCT GGCGGGCCGG 
GGCGGCCCCG GGAGCGCGGG CGGGGCCGGT TTCCCGCGGG AGCCTTCGCC GCCGCAGGCC 
GGTCCCGGCG GGGCCAAGGG CACGGCCGAC TTCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAFA ( NM_201589.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_963883.2 Homo sapiens MAF bZIP transcription factor A (MAFA), mRNA.

Price & Availability↑

CloneID	OHu23130
Clone ID Related Accession (Same CDS sequence)	NM_201589.3 , NM_201589.4
Accession Version	NM_201589.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-24
Organism	Homo sapiens(human)
Product	transcription factor MafA
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC105118.23. On May 17, 2019 this sequence version replaced NM_201589.3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000333480.3/ ENSP00000328364.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGCCGCGG AGCTGGCGAT GGGCGCCGAG CTGCCCAGCA GCCCGCTGGC CATCGAGTAC 
GTCAACGACT TCGACCTGAT GAAGTTCGAG GTGAAGAAGG AGCCTCCCGA GGCCGAGCGC 
TTCTGCCACC GCCTGCCGCC AGGCTCGCTG TCCTCGACGC CGCTCAGCAC GCCCTGCTCC 
TCCGTGCCCT CCTCGCCCAG CTTCTGCGCG CCCAGCCCGG GCACCGGCGG CGGCGGCGGC 
GCGGGGGGCG GCGGCGGCTC GTCTCAGGCC GGGGGCGCCC CCGGGCCGCC GAGCGGGGGC 
CCCGGCGCCG TCGGGGGCAC CTCGGGGAAG CCGGCGCTGG AGGATCTGTA CTGGATGAGC 
GGCTACCAGC ATCACCTCAA CCCCGAGGCG CTCAACCTGA CGCCCGAGGA CGCGGTGGAG 
GCGCTCATCG GCAGCGGCCA CCACGGCGCG CACCACGGCG CGCACCACCC GGCGGCCGCC 
GCAGCCTACG AGGCTTTCCG CGGCCCGGGC TTCGCGGGCG GCGGCGGAGC GGACGACATG 
GGCGCCGGCC ACCACCACGG CGCGCACCAC GCCGCCCACC ATCACCACGC CGCCCACCAC 
CACCACCACC ACCACCACCA CCATGGCGGC GCGGGACACG GCGGTGGCGC GGGCCACCAC 
GTGCGCCTGG AGGAGCGCTT CTCCGACGAC CAGCTGGTGT CCATGTCGGT GCGCGAGCTG 
AACCGGCAGC TCCGCGGCTT CAGCAAGGAG GAGGTCATCC GGCTCAAGCA GAAGCGGCGC 
ACGCTCAAGA ACCGCGGCTA CGCGCAGTCC TGCCGCTTCA AGCGGGTGCA GCAGCGGCAC 
ATTCTGGAGA GCGAGAAGTG CCAACTCCAG AGCCAGGTGG AGCAGCTGAA GCTGGAGGTG 
GGGCGCCTGG CCAAAGAGCG GGACCTGTAC AAGGAGAAAT ACGAGAAGCT GGCGGGCCGG 
GGCGGCCCCG GGAGCGCGGG CGGGGCCGGT TTCCCGCGGG AGCCTTCGCC GCCGCAGGCC 
GGTCCCGGCG GGGCCAAGGG CACGGCCGAC TTCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963883.2
CDS	327..1388
Translation	MAAELAMGAELPSSPLAIEYVNDFDLMKFEVKKEPPEAERFCHRLPPGSLSSTPLSTPCSSVPSSPSFCAPSPGTGGGGGAGGGGGSSQAGGAPGPPSGGPGAVGGTSGKPALEDLYWMSGYQHHLNPEALNLTPEDAVEALIGSGHHGAHHGAHHPAAAAAYEAFRGPGFAGGGGADDMGAGHHHGAHHAAHHHHAAHHHHHHHHHHGGAGHGGGAGHHVRLEERFSDDQLVSMSVRELNRQLRGFSKEEVIRLKQKRRTLKNRGYAQSCRFKRVQQRHILESEKCQLQSQVEQLKLEVGRLAKERDLYKEKYEKLAGRGGPGSAGGAGFPREPSPPQAGPGGAKGTADFFL

Target ORF information:

RefSeq Version	NM_201589.4
Organism	Homo sapiens(human)
Definition	Homo sapiens MAF bZIP transcription factor A (MAFA), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201589.4

ORF Insert Sequence:

ATGGCCGCGG AGCTGGCGAT GGGCGCCGAG CTGCCCAGCA GCCCGCTGGC CATCGAGTAC 
GTCAACGACT TCGACCTGAT GAAGTTCGAG GTGAAGAAGG AGCCTCCCGA GGCCGAGCGC 
TTCTGCCACC GCCTGCCGCC AGGCTCGCTG TCCTCGACGC CGCTCAGCAC GCCCTGCTCC 
TCCGTGCCCT CCTCGCCCAG CTTCTGCGCG CCCAGCCCGG GCACCGGCGG CGGCGGCGGC 
GCGGGGGGCG GCGGCGGCTC GTCTCAGGCC GGGGGCGCCC CCGGGCCGCC GAGCGGGGGC 
CCCGGCGCCG TCGGGGGCAC CTCGGGGAAG CCGGCGCTGG AGGATCTGTA CTGGATGAGC 
GGCTACCAGC ATCACCTCAA CCCCGAGGCG CTCAACCTGA CGCCCGAGGA CGCGGTGGAG 
GCGCTCATCG GCAGCGGCCA CCACGGCGCG CACCACGGCG CGCACCACCC GGCGGCCGCC 
GCAGCCTACG AGGCTTTCCG CGGCCCGGGC TTCGCGGGCG GCGGCGGAGC GGACGACATG 
GGCGCCGGCC ACCACCACGG CGCGCACCAC GCCGCCCACC ATCACCACGC CGCCCACCAC 
CACCACCACC ACCACCACCA CCATGGCGGC GCGGGACACG GCGGTGGCGC GGGCCACCAC 
GTGCGCCTGG AGGAGCGCTT CTCCGACGAC CAGCTGGTGT CCATGTCGGT GCGCGAGCTG 
AACCGGCAGC TCCGCGGCTT CAGCAAGGAG GAGGTCATCC GGCTCAAGCA GAAGCGGCGC 
ACGCTCAAGA ACCGCGGCTA CGCGCAGTCC TGCCGCTTCA AGCGGGTGCA GCAGCGGCAC 
ATTCTGGAGA GCGAGAAGTG CCAACTCCAG AGCCAGGTGG AGCAGCTGAA GCTGGAGGTG 
GGGCGCCTGG CCAAAGAGCG GGACCTGTAC AAGGAGAAAT ACGAGAAGCT GGCGGGCCGG 
GGCGGCCCCG GGAGCGCGGG CGGGGCCGGT TTCCCGCGGG AGCCTTCGCC GCCGCAGGCC 
GGTCCCGGCG GGGCCAAGGG CACGGCCGAC TTCTTCCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Proceedings of the National Academy of Sciences of the United States of America(2018 Jan)
Iacovazzo D,Flanagan SE,Walker E,Quezado R,de Sousa Barros FA,Caswell R,Johnson MB,Wakeling M,Br?ndle M,Guo M,Dang MN,Gabrovska P,Niederle B,Christ E,Jenni S,Sipos B,Nieser M,Frilling A,Dhatariya K,Chanson P,de Herder WW,Konukiewitz B,Kl?ppel G,Stein R,Korbonits M,Ellard S

PDX1, Neurogenin-3, and MAFA: critical transcription regulators for beta cell development and regeneration.
Stem cell research & therapy8(1)240(2017 Nov)
Zhu Y,Liu Q,Zhou Z,Ikeda Y

miR-204 is associated with an endocrine phenotype in human pancreatic islets but does not regulate the insulin mRNA through MAFA.
Scientific reports7(1)14051(2017 Oct)
Marzinotto I,Pellegrini S,Brigatti C,Nano R,Melzi R,Mercalli A,Liberati D,Sordi V,Ferrari M,Falconi M,Doglioni C,Ravassard P,Piemonti L,Lampasona V

Inhibition of the deubiquitinase USP5 leads to c-Maf protein degradation and myeloma cell apoptosis.
Cell death & disease8(9)e3058(2017 Sep)
Wang S,Juan J,Zhang Z,Du Y,Xu Y,Tong J,Cao B,Moran MF,Zeng Y,Mao X

MafA-Controlled Nicotinic Receptor Expression Is Essential for Insulin Secretion and Is Impaired in Patients with Type 2 Diabetes.
Cell reports14(8)1991-2002(2016 Mar)
Ganic E,Singh T,Luan C,Fadista J,Johansson JK,Cyphert HA,Bennet H,Storm P,Prost G,Ahlenius H,Renstr?m E,Stein R,Groop L,Fex M,Artner I

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MAFA cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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