PCARE cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PCARE
Entrez Gene ID	388939
Full Name	chromosome 2 open reading frame 71
Synonyms	RP54
General protein information	Preferred Names chromosome 2 open reading frame 71 Names uncharacterized protein C2orf71
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p23.2
Summary	The protein encoded by this gene is highly expressed in photoreceptors and may associate with the primary cilium of the outer segment. The encoded protein appears to undergo post-translational lipid modification. Nonsense and missense variants of this gene appear to cause a recessive form of retinitis pigmentosa. [provided by RefSeq, Jun 2010].
Disorder MIM:	613425
Disorder Html:	Retinitis pigmentosa 54, 613428 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001029883.2	NP_001025054.1	uncharacterized protein C2orf71
XM_011532826.1	XP_011531128.1	uncharacterized protein C2orf71 isoform X1
NM_001029883.3	NP_001025054.1	photoreceptor cilium actin regulator

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	RGD1304963	XP_006239833.1
Gallus gallus (chicken)	C3H2orf71	XP_004940245.1
Canis lupus familiaris (dog)	C17H2orf71	XP_003432228.1
	LOC703862	XP_001102943.1
Xenopus tropicalis (tropical clawed frog)	LOC101732635	XP_004914984.1
Homo sapiens (human)	C2orf71	NP_001025054.1
Mus musculus (house mouse)	BC027072	NP_666194.2
Danio rerio (zebrafish)	LOC100537793	XP_003200429.2
Pan troglodytes (chimpanzee)	C2AH2orf71	XP_515380.2
Bos taurus (cattle)	C11H2orf71	XP_002691539.1

Gene Ontology
Bibliography

Related articles in PubMed

C2orf71 Mutations as a Frequent Cause of Autosomal-Recessive Retinitis Pigmentosa: Clinical Analysis and Presentation of 8 Novel Mutations.
Gerth-Kahlert C, Tiwari A, Hanson JVM, Batmanabane V, Traboulsi E, Pennesi ME, Al-Qahtani AA, Lam BL, Heckenlively J, Zweifel SA, Vincent A, Fierz F, Barthelmes D, Branham K, Khan N, Bahr A, Baehr L, Magyar I, Koller S, Azzarello-Burri S, Niedrist D, Heon E, Berger W
Investigative ophthalmology & visual science58(10)3840-3850(2017 Aug)

Putative digenic inheritance of heterozygous RP1L1 and C2orf71 null mutations in syndromic retinal dystrophy.
Liu YP, Bosch DG, Siemiatkowska AM, Rendtorff ND, Boonstra FN, M?ller C, Tranebj?rg L, Katsanis N, Cremers FP
Ophthalmic genetics38(2)127-132(2017 Mar-Apr)

A homozygous nonsense CEP250 mutation combined with a heterozygous nonsense C2orf71 mutation is associated with atypical Usher syndrome.
Khateb S, Zelinger L, Mizrahi-Meissonnier L, Ayuso C, Koenekoop RK, Laxer U, Gross M, Banin E, Sharon D
Journal of medical genetics51(7)460-9(2014 Jul)

Novel C2orf71 mutations account for ?1% of cases in a large French arRP cohort.
Audo I, Lancelot ME, Mohand-Sa?d S, Antonio A, Germain A, Sahel JA, Bhattacharya SS, Zeitz C
Human mutation32(4)E2091-103(2011 Apr)

A survey of DNA variation of C2ORF71 in probands with progressive autosomal recessive retinal degeneration and controls.
Sergouniotis PI, Li Z, Mackay DS, Wright GA, Borman AD, Devery SR, Moore AT, Webster AR
Investigative ophthalmology & visual science52(3)1880-6(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

On the basis of our multicenter analysis, C2orf71 might represent a more frequently mutated gene in autosomal recessive retinitis pigmentosa in some populations.
Title: C2orf71 Mutations as a Frequent Cause of Autosomal-Recessive Retinitis Pigmentosa: Clinical Analysis and Presentation of 8 Novel Mutations.

We propose that the combination of heterozygous loss-of-function mutations in these genes drives syndromic retinal dystrophy, likely through the genetic interaction of at least two loci.
Title: Putative digenic inheritance of heterozygous RP1L1 and C2orf71 null mutations in syndromic retinal dystrophy.

A homozygous nonsense CEP250 mutation, in combination with a heterozygous C2orf71 nonsense mutation, causes an atypical form of Usher syndrome, characterised by early-onset sensorineural hearing loss and a relatively mild retinitis pigmentosa.
Title: A homozygous nonsense CEP250 mutation combined with a heterozygous nonsense C2orf71 mutation is associated with atypical Usher syndrome.

Novel C2orf71 mutations account for approximately 1% of cases in a large French arRP cohort.
Title: Novel C2orf71 mutations account for ?1% of cases in a large French arRP cohort.

Observational study of gene-disease association. (HuGE Navigator)
Title: A survey of DNA variation of C2ORF71 in probands with progressive autosomal recessive retinal degeneration and controls.

The following PCARE gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PCARE cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21482	NM_001029883.3 Latest version!	Homo sapiens photoreceptor cilium actin regulator (PCARE), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu21482	XM_011532826.1 Latest version!	Homo sapiens chromosome 2 open reading frame 71 (C2orf71), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
View Old Accession Versions >>
OHu21482	NM_001029883.2	Homo sapiens chromosome 2 open reading frame 71 (C2orf71), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PCARE ( NM_001029883.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025054.1 Homo sapiens chromosome 2 open reading frame 71 (C2orf71), mRNA.

Price & Availability↑

CloneID	OHu21482
Clone ID Related Accession (Same CDS sequence)	NM_001029883.2 , XM_011532826.1 , NM_001029883.3
Accession Version	NM_001029883.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	uncharacterized protein C2orf71
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105398.3, AK092250.1 and BM669582.1. This sequence is a reference standard in the RefSeqGene project. On Nov 9, 2011 this sequence version replaced NM_001029883.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK092250.1, DA742287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGTGTA CACCTTCACA CAGTGACCTT GTAAACAGCG TTGCAAAGAG TGGCATTCAG 
TTCTTGAAAA AGCCCAAAGC AATTCGGCCA GGATGTCAGG GCGGAAGTGA AAGAGGTTCC 
ATCCCTTTGC TGGTTAAAAA CTCCACCTGC TATGACGCTG GGGAGGGCCT GGCAGAGGAG 
CAGCCAAGTC CCAGGAGGAA CCAAACCACA GCTAAAGGTC TTTGTCAGCT CATGGGAGAT 
CCTGCTTCAG GCAAAAGGAA AGATATGGAA GGACTGATCC CAGGAACCAA AACCTCTTCA 
TCCCAGCTGA ACAAATCACA AAGCCACATG GCTAAGGATA TTCCGTTCAA GACACAGGGT 
TCCCATGGAT CACAAGGGGC AGACTTTTCT GGAGATGAGA GTGAGGAAAG TAGTACCCAA 
GATACTTCCA AATGGAAAAG GACAGCAAAA TGTCACACGT CAAGCACACA GAGCCACTGC 
TACCAAACCA TCCACCCTGC TCATGAGCCT GAAGGCAAAG TGGACTTCCC GGAGCCTCTG 
GTAAAGGCCC ACCAGCAGGC TTACACCTAT CTACACTCCA GCCTCTCCAA ATATGAAGCA 
ATTCTGTGCA TCATCCATCA GGCCACCCAG ACCCGGGAGC TGCTGCAGCC CATGGTCAGC 
TTCTTGCTGC TGTGCTTTGA GGAGATCAGC CAGCTGTTGG GGGAGATCTC CAAGGATGGA 
GAAGTGCTCC TGCAGGAAGT CAGGGAGGAT CTGGCTTGGC CTTTGAAGAA AAGAGAGCCC 
CAGGAGCAGC CAAATCTCCT GCAACAGCTG CTACAGTACA CAGTCAGCAA GCTGCAGGTG 
CTCAATGGCA CAGTGGCCTC GCTCACCGGC AGCTTCCTGG AGGGCTCCAG CAGCTACCTC 
CACTCCACTG CAACCCACTT GGAAAATAAG CTGAGCACAA AAAGGAATGT GGATGAACGC 
CTCCTGAGGG CTCTGAGGCA GCTAGAGAGC CTGGCGAGTG GCTGTGGCGA CCCTGGGGTG 
CAGGGTCTCC CCTTATGCTC TGAGGACAGT GGCATTGGTG CTGACAATGA GTCCGTGCAG 
TCGGTGGACA AGCTGGGCAA GCAAACCAGC TGGGACCTTG CACCAGAGCC CGAAGAATGG 
AAGTCGGTGA CTTCACCCCA CACAGAGGCC AGGCAGTCAG GACACACCTG GCAGCAAAGT 
CCATTCTGTT TGGGCTCAGG CAGACCCCAG GACTGCCTGC TCTCAGGGGC TCCTATGGCA 
AAGGTTCAGC CACGAGCACA GGACGAAGCA AGGAGCCCAT GCCTCTCCAG TACAAGCCCA 
GAAAATATCA CCTCCCCACC TTTGAAGCTG GGGACAAGCA CCCCATGTGA TTCCTTTGGG 
ATTGGGGTCT CTGTGGAACC ACACCTTTCC AAAACCTCCA GGCCGATGGA CGCTTCATCT 
CTTAGTGACA GCGAGGACAG CAGCCCAGAG GAGGAGGAGG AAGACAAAAT GAGCAGCATG 
AGTCTGTGTG CCTGGCAGGA AAAAACTCCA CATTCAAGGC CACAATCTTC ACCTGCTGAC 
CGGGAAAGCC CATTTCAGGC CCGCACCAGG AGGCTTAGGA GCCTCCAGGC CCAGGAAATG 
ATTCTGAAGA TGAAGGAGTC AATCAGCGAA AGGATCAAGT TTGTCCCTGT GCCCTGTGGG 
CACCAGGACT GGTCTGAGGA GGAGGAGGGG AGGACAGTGG TCCCCCCAAG ACCTAGCACG 
GTAAGTGGCA GCAGGAGGGC CCCTGAGAGG CAGACGAGGT CCCAGTCAGA GTCGTGTCTC 
CAGAGTCACG TGGAGGACCC CACCTTTCAG GAGCTGCGAA GGGTCCAGAG GGACCTCAGT 
CAGAAGCTGG AGGCATTTTA TGCCCTGGGT GCCAAAGGGC AGGGGCAGAG CCAGGAGCAA 
ATTCTGCAGC CCAGAGCAGC CGCCGTGTGG CCCAATGGCA CCTGCAGGGT CAGTCCAAGC 
AACACCACCA GCAGGCTCAA GGCATCCCTC ACCAAGAACT TCAGTATTTT GCCTAGTCAG 
GACAAGAGCA TCTTGCAGAA ATGCAATCCC CATCCTGAGG ACGAACAAGG CAAAGCTGGG 
AAGCTTCCAA ATGCCATCCC ATCAGGAGAG GTCAGTGAGG CTGCCAAGGC CACAGACTGG 
AATGTCAGAG GCTGTCCCAC CAGAACATCC GTCAAGAAGC TTATTGAAAC TTTCAGTCCC 
ACGGAGAGTC TGAGGATGCT GGGGGACTCT AAGGACGCTG GGGCAAGTCC CTGCCTCAGG 
AATTGCATCA TGCCCCCCAG ATTTCCCAAG TACACAGGGC TTGCCCCTTT GTATCCGAAG 
CCCCAAATTT CTCCAGCATC AGGCAGAGAA TCTCTCAAAA TGGGCATAGG CTGGAAGCCC 
TTAGCACCTA TCTTTCCCCC TCTGCCTAAA GCAGAAGCAG CCAAGAGTGA GGAGCTCAGC 
TGTGAAATGG AGGGGAACCT CGAGCACCTC CCTCCACCGC CTATGGAAGT TCTGATGGAC 
AAATCATTCG CTTCTCTGGA GTCCCCAGAA AGCAGCAAGT CCACAGAGAA CTCCCCCAAG 
GAAACCCAGG AGCCAGGGCC GGGAGAGGCT GGCCCCACCA GGAGAACATG GGCTTCCCCA 
AAGCTGAGGG CCTCTGTGAG CCCCCTGGAC TTGCTGCCCA GCAAGAGCAC CGCCAGCCTG 
ACCAAGCCTC ACAGCACAGG GCCAGGGAGT GGCAGGAGCA GCTGCCAGCC CAGGAAGCCA 
GCCCTGGACC TGAGCAGCCC ACCAGCCACC AGCCAAAGCC CCGAGGTGAA GGGTGGGACT 
TGGAGTCAGG CAGAGAAGGC CACCAGCCTC TACAGGCAGC CCCGGAAGGC CATCGCCTGG 
CACCACTCCG GCCCTCCATC TGGACAAAAC AGGACCTCAG AGTCCAGCCT GGCCAGACCA 
AGGCAGAGCC GAGAGAGAAG CCCCCCTGTG GGCAGAAAGG CCTCTCCCAC GAGGACACAC 
TGGGTGCCTC AAGCAGACAA GAGGCGCCGG AGCCTTCCCT CCTCTTACAG ACCTGCCCAG 
CCAAGCCCCT CTGCTGTGCA GACGCCCCCC AGCCCACCTG TGAGCCCCAG GGTGCTAAGC 
CCACCCACCA CAAAGCGGCG AACTTCCCCA CCGCACCAGC CCAAGTTGCC CAACCCTCCC 
CCCGAGAGTG CACCTGCTCA GTGCAAGGTC CCCAGCCCCC CAACCCAGCA CCCAGAAGCA 
AGCCCCCCTT TCTCGATTCC CTCCCCATCA CCCCCAATGT CCCCTTCTCA GGAGCACAAG 
GAAACAAGAG ACTCTGAAGA CAGCCAAGCA GTCATAGCCA AAGTGTCTGG GAACACACAT 
TCCATATTCT GCCCAGCTAC CTCCTCTCTG TTTGAAGCTA AACCGCCACT CTCAACAGCC 
CACCCACTGA CCCCACCATC GCTGCCGCCA GAGGCTGGGG GCCCTCTCGG GAACCCAGCA 
GAATGCTGGA AGAACAGCTC AGGGCCTTGG CTGAGAGCAG ACTCGCAGCG GAGAGCAGCT 
CTGTGTGCCC TCAACCCTCT GCCTTTCCTC AGGAGGACAG CTTCTGACCG CCAGCCAGGT 
GGCCGACCGC AGCCTCCCAC CTTGGACCCC ACCAGCACCT CTTATGAATC CCAGCTCGGC 
CAGAACAGCA GCAGCGAGGA GAGCCCTAAG AAGGACACAG AGCCGGGGAG CAGCCCCTGT 
TCCCCTGAAC TGCAGGGCGG CACCAGGCGT GCATCTCCCC CAGAGTTCTG TGTGCTGGGC 
CACGGGCTGC AACCGGAGCC TCGGACCGGC CACATCCAGG ACAAATCCCA GCCAGAGGCG 
CAGCCCCAGC AAGAGGAGGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025054.1
CDS	1..3867
Translation	MGCTPSHSDLVNSVAKSGIQFLKKPKAIRPGCQGGSERGSIPLLVKNSTCYDAGEGLAEEQPSPRRNQTTAKGLCQLMGDPASGKRKDMEGLIPGTKTSSSQLNKSQSHMAKDIPFKTQGSHGSQGADFSGDESEESSTQDTSKWKRTAKCHTSSTQSHCYQTIHPAHEPEGKVDFPEPLVKAHQQAYTYLHSSLSKYEAILCIIHQATQTRELLQPMVSFLLLCFEEISQLLGEISKDGEVLLQEVREDLAWPLKKREPQEQPNLLQQLLQYTVSKLQVLNGTVASLTGSFLEGSSSYLHSTATHLENKLSTKRNVDERLLRALRQLESLASGCGDPGVQGLPLCSEDSGIGADNESVQSVDKLGKQTSWDLAPEPEEWKSVTSPHTEARQSGHTWQQSPFCLGSGRPQDCLLSGAPMAKVQPRAQDEARSPCLSSTSPENITSPPLKLGTSTPCDSFGIGVSVEPHLSKTSRPMDASSLSDSEDSSPEEEEEDKMSSMSLCAWQEKTPHSRPQSSPADRESPFQARTRRLRSLQAQEMILKMKESISERIKFVPVPCGHQDWSEEEEGRTVVPPRPSTVSGSRRAPERQTRSQSESCLQSHVEDPTFQELRRVQRDLSQKLEAFYALGAKGQGQSQEQILQPRAAAVWPNGTCRVSPSNTTSRLKASLTKNFSILPSQDKSILQKCNPHPEDEQGKAGKLPNAIPSGEVSEAAKATDWNVRGCPTRTSVKKLIETFSPTESLRMLGDSKDAGASPCLRNCIMPPRFPKYTGLAPLYPKPQISPASGRESLKMGIGWKPLAPIFPPLPKAEAAKSEELSCEMEGNLEHLPPPPMEVLMDKSFASLESPESSKSTENSPKETQEPGPGEAGPTRRTWASPKLRASVSPLDLLPSKSTASLTKPHSTGPGSGRSSCQPRKPALDLSSPPATSQSPEVKGGTWSQAEKATSLYRQPRKAIAWHHSGPPSGQNRTSESSLARPRQSRERSPPVGRKASPTRTHWVPQADKRRRSLPSSYRPAQPSPSAVQTPPSPPVSPRVLSPPTTKRRTSPPHQPKLPNPPPESAPAQCKVPSPPTQHPEASPPFSIPSPSPPMSPSQEHKETRDSEDSQAVIAKVSGNTHSIFCPATSSLFEAKPPLSTAHPLTPPSLPPEAGGPLGNPAECWKNSSGPWLRADSQRRAALCALNPLPFLRRTASDRQPGGRPQPPTLDPTSTSYESQLGQNSSSEESPKKDTEPGSSPCSPELQGGTRRASPPEFCVLGHGLQPEPRTGHIQDKSQPEAQPQQEEVS

Target ORF information:

RefSeq Version	NM_001029883.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromosome 2 open reading frame 71 (C2orf71), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029883.2

ORF Insert Sequence:

ATGGGGTGTA CACCTTCACA CAGTGACCTT GTAAACAGCG TTGCAAAGAG TGGCATTCAG 
TTCTTGAAAA AGCCCAAAGC AATTCGGCCA GGATGTCAGG GCGGAAGTGA AAGAGGTTCC 
ATCCCTTTGC TGGTTAAAAA CTCCACCTGC TATGACGCTG GGGAGGGCCT GGCAGAGGAG 
CAGCCAAGTC CCAGGAGGAA CCAAACCACA GCTAAAGGTC TTTGTCAGCT CATGGGAGAT 
CCTGCTTCAG GCAAAAGGAA AGATATGGAA GGACTGATCC CAGGAACCAA AACCTCTTCA 
TCCCAGCTGA ACAAATCACA AAGCCACATG GCTAAGGATA TTCCGTTCAA GACACAGGGT 
TCCCATGGAT CACAAGGGGC AGACTTTTCT GGAGATGAGA GTGAGGAAAG TAGTACCCAA 
GATACTTCCA AATGGAAAAG GACAGCAAAA TGTCACACGT CAAGCACACA GAGCCACTGC 
TACCAAACCA TCCACCCTGC TCATGAGCCT GAAGGCAAAG TGGACTTCCC GGAGCCTCTG 
GTAAAGGCCC ACCAGCAGGC TTACACCTAT CTACACTCCA GCCTCTCCAA ATATGAAGCA 
ATTCTGTGCA TCATCCATCA GGCCACCCAG ACCCGGGAGC TGCTGCAGCC CATGGTCAGC 
TTCTTGCTGC TGTGCTTTGA GGAGATCAGC CAGCTGTTGG GGGAGATCTC CAAGGATGGA 
GAAGTGCTCC TGCAGGAAGT CAGGGAGGAT CTGGCTTGGC CTTTGAAGAA AAGAGAGCCC 
CAGGAGCAGC CAAATCTCCT GCAACAGCTG CTACAGTACA CAGTCAGCAA GCTGCAGGTG 
CTCAATGGCA CAGTGGCCTC GCTCACCGGC AGCTTCCTGG AGGGCTCCAG CAGCTACCTC 
CACTCCACTG CAACCCACTT GGAAAATAAG CTGAGCACAA AAAGGAATGT GGATGAACGC 
CTCCTGAGGG CTCTGAGGCA GCTAGAGAGC CTGGCGAGTG GCTGTGGCGA CCCTGGGGTG 
CAGGGTCTCC CCTTATGCTC TGAGGACAGT GGCATTGGTG CTGACAATGA GTCCGTGCAG 
TCGGTGGACA AGCTGGGCAA GCAAACCAGC TGGGACCTTG CACCAGAGCC CGAAGAATGG 
AAGTCGGTGA CTTCACCCCA CACAGAGGCC AGGCAGTCAG GACACACCTG GCAGCAAAGT 
CCATTCTGTT TGGGCTCAGG CAGACCCCAG GACTGCCTGC TCTCAGGGGC TCCTATGGCA 
AAGGTTCAGC CACGAGCACA GGACGAAGCA AGGAGCCCAT GCCTCTCCAG TACAAGCCCA 
GAAAATATCA CCTCCCCACC TTTGAAGCTG GGGACAAGCA CCCCATGTGA TTCCTTTGGG 
ATTGGGGTCT CTGTGGAACC ACACCTTTCC AAAACCTCCA GGCCGATGGA CGCTTCATCT 
CTTAGTGACA GCGAGGACAG CAGCCCAGAG GAGGAGGAGG AAGACAAAAT GAGCAGCATG 
AGTCTGTGTG CCTGGCAGGA AAAAACTCCA CATTCAAGGC CACAATCTTC ACCTGCTGAC 
CGGGAAAGCC CATTTCAGGC CCGCACCAGG AGGCTTAGGA GCCTCCAGGC CCAGGAAATG 
ATTCTGAAGA TGAAGGAGTC AATCAGCGAA AGGATCAAGT TTGTCCCTGT GCCCTGTGGG 
CACCAGGACT GGTCTGAGGA GGAGGAGGGG AGGACAGTGG TCCCCCCAAG ACCTAGCACG 
GTAAGTGGCA GCAGGAGGGC CCCTGAGAGG CAGACGAGGT CCCAGTCAGA GTCGTGTCTC 
CAGAGTCACG TGGAGGACCC CACCTTTCAG GAGCTGCGAA GGGTCCAGAG GGACCTCAGT 
CAGAAGCTGG AGGCATTTTA TGCCCTGGGT GCCAAAGGGC AGGGGCAGAG CCAGGAGCAA 
ATTCTGCAGC CCAGAGCAGC CGCCGTGTGG CCCAATGGCA CCTGCAGGGT CAGTCCAAGC 
AACACCACCA GCAGGCTCAA GGCATCCCTC ACCAAGAACT TCAGTATTTT GCCTAGTCAG 
GACAAGAGCA TCTTGCAGAA ATGCAATCCC CATCCTGAGG ACGAACAAGG CAAAGCTGGG 
AAGCTTCCAA ATGCCATCCC ATCAGGAGAG GTCAGTGAGG CTGCCAAGGC CACAGACTGG 
AATGTCAGAG GCTGTCCCAC CAGAACATCC GTCAAGAAGC TTATTGAAAC TTTCAGTCCC 
ACGGAGAGTC TGAGGATGCT GGGGGACTCT AAGGACGCTG GGGCAAGTCC CTGCCTCAGG 
AATTGCATCA TGCCCCCCAG ATTTCCCAAG TACACAGGGC TTGCCCCTTT GTATCCGAAG 
CCCCAAATTT CTCCAGCATC AGGCAGAGAA TCTCTCAAAA TGGGCATAGG CTGGAAGCCC 
TTAGCACCTA TCTTTCCCCC TCTGCCTAAA GCAGAAGCAG CCAAGAGTGA GGAGCTCAGC 
TGTGAAATGG AGGGGAACCT CGAGCACCTC CCTCCACCGC CTATGGAAGT TCTGATGGAC 
AAATCATTCG CTTCTCTGGA GTCCCCAGAA AGCAGCAAGT CCACAGAGAA CTCCCCCAAG 
GAAACCCAGG AGCCAGGGCC GGGAGAGGCT GGCCCCACCA GGAGAACATG GGCTTCCCCA 
AAGCTGAGGG CCTCTGTGAG CCCCCTGGAC TTGCTGCCCA GCAAGAGCAC CGCCAGCCTG 
ACCAAGCCTC ACAGCACAGG GCCAGGGAGT GGCAGGAGCA GCTGCCAGCC CAGGAAGCCA 
GCCCTGGACC TGAGCAGCCC ACCAGCCACC AGCCAAAGCC CCGAGGTGAA GGGTGGGACT 
TGGAGTCAGG CAGAGAAGGC CACCAGCCTC TACAGGCAGC CCCGGAAGGC CATCGCCTGG 
CACCACTCCG GCCCTCCATC TGGACAAAAC AGGACCTCAG AGTCCAGCCT GGCCAGACCA 
AGGCAGAGCC GAGAGAGAAG CCCCCCTGTG GGCAGAAAGG CCTCTCCCAC GAGGACACAC 
TGGGTGCCTC AAGCAGACAA GAGGCGCCGG AGCCTTCCCT CCTCTTACAG ACCTGCCCAG 
CCAAGCCCCT CTGCTGTGCA GACGCCCCCC AGCCCACCTG TGAGCCCCAG GGTGCTAAGC 
CCACCCACCA CAAAGCGGCG AACTTCCCCA CCGCACCAGC CCAAGTTGCC CAACCCTCCC 
CCCGAGAGTG CACCTGCTCA GTGCAAGGTC CCCAGCCCCC CAACCCAGCA CCCAGAAGCA 
AGCCCCCCTT TCTCGATTCC CTCCCCATCA CCCCCAATGT CCCCTTCTCA GGAGCACAAG 
GAAACAAGAG ACTCTGAAGA CAGCCAAGCA GTCATAGCCA AAGTGTCTGG GAACACACAT 
TCCATATTCT GCCCAGCTAC CTCCTCTCTG TTTGAAGCTA AACCGCCACT CTCAACAGCC 
CACCCACTGA CCCCACCATC GCTGCCGCCA GAGGCTGGGG GCCCTCTCGG GAACCCAGCA 
GAATGCTGGA AGAACAGCTC AGGGCCTTGG CTGAGAGCAG ACTCGCAGCG GAGAGCAGCT 
CTGTGTGCCC TCAACCCTCT GCCTTTCCTC AGGAGGACAG CTTCTGACCG CCAGCCAGGT 
GGCCGACCGC AGCCTCCCAC CTTGGACCCC ACCAGCACCT CTTATGAATC CCAGCTCGGC 
CAGAACAGCA GCAGCGAGGA GAGCCCTAAG AAGGACACAG AGCCGGGGAG CAGCCCCTGT 
TCCCCTGAAC TGCAGGGCGG CACCAGGCGT GCATCTCCCC CAGAGTTCTG TGTGCTGGGC 
CACGGGCTGC AACCGGAGCC TCGGACCGGC CACATCCAGG ACAAATCCCA GCCAGAGGCG 
CAGCCCCAGC AAGAGGAGGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCARE ( NM_001029883.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025054.1 Homo sapiens photoreceptor cilium actin regulator (PCARE), mRNA.

Price & Availability↑

CloneID	OHu21482
Clone ID Related Accession (Same CDS sequence)	NM_001029883.2 , XM_011532826.1 , NM_001029883.3
Accession Version	NM_001029883.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-21
Organism	Homo sapiens(human)
Product	photoreceptor cilium actin regulator
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC105398.3, AK092250.1 and BM669582.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_001029883.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AK092250.1, DA742287.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000331664.6/ ENSP00000332809.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGGTGTA CACCTTCACA CAGTGACCTT GTAAACAGCG TTGCAAAGAG TGGCATTCAG 
TTCTTGAAAA AGCCCAAAGC AATTCGGCCA GGATGTCAGG GCGGAAGTGA AAGAGGTTCC 
ATCCCTTTGC TGGTTAAAAA CTCCACCTGC TATGACGCTG GGGAGGGCCT GGCAGAGGAG 
CAGCCAAGTC CCAGGAGGAA CCAAACCACA GCTAAAGGTC TTTGTCAGCT CATGGGAGAT 
CCTGCTTCAG GCAAAAGGAA AGATATGGAA GGACTGATCC CAGGAACCAA AACCTCTTCA 
TCCCAGCTGA ACAAATCACA AAGCCACATG GCTAAGGATA TTCCGTTCAA GACACAGGGT 
TCCCATGGAT CACAAGGGGC AGACTTTTCT GGAGATGAGA GTGAGGAAAG TAGTACCCAA 
GATACTTCCA AATGGAAAAG GACAGCAAAA TGTCACACGT CAAGCACACA GAGCCACTGC 
TACCAAACCA TCCACCCTGC TCATGAGCCT GAAGGCAAAG TGGACTTCCC GGAGCCTCTG 
GTAAAGGCCC ACCAGCAGGC TTACACCTAT CTACACTCCA GCCTCTCCAA ATATGAAGCA 
ATTCTGTGCA TCATCCATCA GGCCACCCAG ACCCGGGAGC TGCTGCAGCC CATGGTCAGC 
TTCTTGCTGC TGTGCTTTGA GGAGATCAGC CAGCTGTTGG GGGAGATCTC CAAGGATGGA 
GAAGTGCTCC TGCAGGAAGT CAGGGAGGAT CTGGCTTGGC CTTTGAAGAA AAGAGAGCCC 
CAGGAGCAGC CAAATCTCCT GCAACAGCTG CTACAGTACA CAGTCAGCAA GCTGCAGGTG 
CTCAATGGCA CAGTGGCCTC GCTCACCGGC AGCTTCCTGG AGGGCTCCAG CAGCTACCTC 
CACTCCACTG CAACCCACTT GGAAAATAAG CTGAGCACAA AAAGGAATGT GGATGAACGC 
CTCCTGAGGG CTCTGAGGCA GCTAGAGAGC CTGGCGAGTG GCTGTGGCGA CCCTGGGGTG 
CAGGGTCTCC CCTTATGCTC TGAGGACAGT GGCATTGGTG CTGACAATGA GTCCGTGCAG 
TCGGTGGACA AGCTGGGCAA GCAAACCAGC TGGGACCTTG CACCAGAGCC CGAAGAATGG 
AAGTCGGTGA CTTCACCCCA CACAGAGGCC AGGCAGTCAG GACACACCTG GCAGCAAAGT 
CCATTCTGTT TGGGCTCAGG CAGACCCCAG GACTGCCTGC TCTCAGGGGC TCCTATGGCA 
AAGGTTCAGC CACGAGCACA GGACGAAGCA AGGAGCCCAT GCCTCTCCAG TACAAGCCCA 
GAAAATATCA CCTCCCCACC TTTGAAGCTG GGGACAAGCA CCCCATGTGA TTCCTTTGGG 
ATTGGGGTCT CTGTGGAACC ACACCTTTCC AAAACCTCCA GGCCGATGGA CGCTTCATCT 
CTTAGTGACA GCGAGGACAG CAGCCCAGAG GAGGAGGAGG AAGACAAAAT GAGCAGCATG 
AGTCTGTGTG CCTGGCAGGA AAAAACTCCA CATTCAAGGC CACAATCTTC ACCTGCTGAC 
CGGGAAAGCC CATTTCAGGC CCGCACCAGG AGGCTTAGGA GCCTCCAGGC CCAGGAAATG 
ATTCTGAAGA TGAAGGAGTC AATCAGCGAA AGGATCAAGT TTGTCCCTGT GCCCTGTGGG 
CACCAGGACT GGTCTGAGGA GGAGGAGGGG AGGACAGTGG TCCCCCCAAG ACCTAGCACG 
GTAAGTGGCA GCAGGAGGGC CCCTGAGAGG CAGACGAGGT CCCAGTCAGA GTCGTGTCTC 
CAGAGTCACG TGGAGGACCC CACCTTTCAG GAGCTGCGAA GGGTCCAGAG GGACCTCAGT 
CAGAAGCTGG AGGCATTTTA TGCCCTGGGT GCCAAAGGGC AGGGGCAGAG CCAGGAGCAA 
ATTCTGCAGC CCAGAGCAGC CGCCGTGTGG CCCAATGGCA CCTGCAGGGT CAGTCCAAGC 
AACACCACCA GCAGGCTCAA GGCATCCCTC ACCAAGAACT TCAGTATTTT GCCTAGTCAG 
GACAAGAGCA TCTTGCAGAA ATGCAATCCC CATCCTGAGG ACGAACAAGG CAAAGCTGGG 
AAGCTTCCAA ATGCCATCCC ATCAGGAGAG GTCAGTGAGG CTGCCAAGGC CACAGACTGG 
AATGTCAGAG GCTGTCCCAC CAGAACATCC GTCAAGAAGC TTATTGAAAC TTTCAGTCCC 
ACGGAGAGTC TGAGGATGCT GGGGGACTCT AAGGACGCTG GGGCAAGTCC CTGCCTCAGG 
AATTGCATCA TGCCCCCCAG ATTTCCCAAG TACACAGGGC TTGCCCCTTT GTATCCGAAG 
CCCCAAATTT CTCCAGCATC AGGCAGAGAA TCTCTCAAAA TGGGCATAGG CTGGAAGCCC 
TTAGCACCTA TCTTTCCCCC TCTGCCTAAA GCAGAAGCAG CCAAGAGTGA GGAGCTCAGC 
TGTGAAATGG AGGGGAACCT CGAGCACCTC CCTCCACCGC CTATGGAAGT TCTGATGGAC 
AAATCATTCG CTTCTCTGGA GTCCCCAGAA AGCAGCAAGT CCACAGAGAA CTCCCCCAAG 
GAAACCCAGG AGCCAGGGCC GGGAGAGGCT GGCCCCACCA GGAGAACATG GGCTTCCCCA 
AAGCTGAGGG CCTCTGTGAG CCCCCTGGAC TTGCTGCCCA GCAAGAGCAC CGCCAGCCTG 
ACCAAGCCTC ACAGCACAGG GCCAGGGAGT GGCAGGAGCA GCTGCCAGCC CAGGAAGCCA 
GCCCTGGACC TGAGCAGCCC ACCAGCCACC AGCCAAAGCC CCGAGGTGAA GGGTGGGACT 
TGGAGTCAGG CAGAGAAGGC CACCAGCCTC TACAGGCAGC CCCGGAAGGC CATCGCCTGG 
CACCACTCCG GCCCTCCATC TGGACAAAAC AGGACCTCAG AGTCCAGCCT GGCCAGACCA 
AGGCAGAGCC GAGAGAGAAG CCCCCCTGTG GGCAGAAAGG CCTCTCCCAC GAGGACACAC 
TGGGTGCCTC AAGCAGACAA GAGGCGCCGG AGCCTTCCCT CCTCTTACAG ACCTGCCCAG 
CCAAGCCCCT CTGCTGTGCA GACGCCCCCC AGCCCACCTG TGAGCCCCAG GGTGCTAAGC 
CCACCCACCA CAAAGCGGCG AACTTCCCCA CCGCACCAGC CCAAGTTGCC CAACCCTCCC 
CCCGAGAGTG CACCTGCTCA GTGCAAGGTC CCCAGCCCCC CAACCCAGCA CCCAGAAGCA 
AGCCCCCCTT TCTCGATTCC CTCCCCATCA CCCCCAATGT CCCCTTCTCA GGAGCACAAG 
GAAACAAGAG ACTCTGAAGA CAGCCAAGCA GTCATAGCCA AAGTGTCTGG GAACACACAT 
TCCATATTCT GCCCAGCTAC CTCCTCTCTG TTTGAAGCTA AACCGCCACT CTCAACAGCC 
CACCCACTGA CCCCACCATC GCTGCCGCCA GAGGCTGGGG GCCCTCTCGG GAACCCAGCA 
GAATGCTGGA AGAACAGCTC AGGGCCTTGG CTGAGAGCAG ACTCGCAGCG GAGAGCAGCT 
CTGTGTGCCC TCAACCCTCT GCCTTTCCTC AGGAGGACAG CTTCTGACCG CCAGCCAGGT 
GGCCGACCGC AGCCTCCCAC CTTGGACCCC ACCAGCACCT CTTATGAATC CCAGCTCGGC 
CAGAACAGCA GCAGCGAGGA GAGCCCTAAG AAGGACACAG AGCCGGGGAG CAGCCCCTGT 
TCCCCTGAAC TGCAGGGCGG CACCAGGCGT GCATCTCCCC CAGAGTTCTG TGTGCTGGGC 
CACGGGCTGC AACCGGAGCC TCGGACCGGC CACATCCAGG ACAAATCCCA GCCAGAGGCG 
CAGCCCCAGC AAGAGGAGGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025054.1
CDS	263..4129
Translation	MGCTPSHSDLVNSVAKSGIQFLKKPKAIRPGCQGGSERGSIPLLVKNSTCYDAGEGLAEEQPSPRRNQTTAKGLCQLMGDPASGKRKDMEGLIPGTKTSSSQLNKSQSHMAKDIPFKTQGSHGSQGADFSGDESEESSTQDTSKWKRTAKCHTSSTQSHCYQTIHPAHEPEGKVDFPEPLVKAHQQAYTYLHSSLSKYEAILCIIHQATQTRELLQPMVSFLLLCFEEISQLLGEISKDGEVLLQEVREDLAWPLKKREPQEQPNLLQQLLQYTVSKLQVLNGTVASLTGSFLEGSSSYLHSTATHLENKLSTKRNVDERLLRALRQLESLASGCGDPGVQGLPLCSEDSGIGADNESVQSVDKLGKQTSWDLAPEPEEWKSVTSPHTEARQSGHTWQQSPFCLGSGRPQDCLLSGAPMAKVQPRAQDEARSPCLSSTSPENITSPPLKLGTSTPCDSFGIGVSVEPHLSKTSRPMDASSLSDSEDSSPEEEEEDKMSSMSLCAWQEKTPHSRPQSSPADRESPFQARTRRLRSLQAQEMILKMKESISERIKFVPVPCGHQDWSEEEEGRTVVPPRPSTVSGSRRAPERQTRSQSESCLQSHVEDPTFQELRRVQRDLSQKLEAFYALGAKGQGQSQEQILQPRAAAVWPNGTCRVSPSNTTSRLKASLTKNFSILPSQDKSILQKCNPHPEDEQGKAGKLPNAIPSGEVSEAAKATDWNVRGCPTRTSVKKLIETFSPTESLRMLGDSKDAGASPCLRNCIMPPRFPKYTGLAPLYPKPQISPASGRESLKMGIGWKPLAPIFPPLPKAEAAKSEELSCEMEGNLEHLPPPPMEVLMDKSFASLESPESSKSTENSPKETQEPGPGEAGPTRRTWASPKLRASVSPLDLLPSKSTASLTKPHSTGPGSGRSSCQPRKPALDLSSPPATSQSPEVKGGTWSQAEKATSLYRQPRKAIAWHHSGPPSGQNRTSESSLARPRQSRERSPPVGRKASPTRTHWVPQADKRRRSLPSSYRPAQPSPSAVQTPPSPPVSPRVLSPPTTKRRTSPPHQPKLPNPPPESAPAQCKVPSPPTQHPEASPPFSIPSPSPPMSPSQEHKETRDSEDSQAVIAKVSGNTHSIFCPATSSLFEAKPPLSTAHPLTPPSLPPEAGGPLGNPAECWKNSSGPWLRADSQRRAALCALNPLPFLRRTASDRQPGGRPQPPTLDPTSTSYESQLGQNSSSEESPKKDTEPGSSPCSPELQGGTRRASPPEFCVLGHGLQPEPRTGHIQDKSQPEAQPQQEEVS

Target ORF information:

RefSeq Version	NM_001029883.3
Organism	Homo sapiens(human)
Definition	Homo sapiens photoreceptor cilium actin regulator (PCARE), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001029883.3

ORF Insert Sequence:

ATGGGGTGTA CACCTTCACA CAGTGACCTT GTAAACAGCG TTGCAAAGAG TGGCATTCAG 
TTCTTGAAAA AGCCCAAAGC AATTCGGCCA GGATGTCAGG GCGGAAGTGA AAGAGGTTCC 
ATCCCTTTGC TGGTTAAAAA CTCCACCTGC TATGACGCTG GGGAGGGCCT GGCAGAGGAG 
CAGCCAAGTC CCAGGAGGAA CCAAACCACA GCTAAAGGTC TTTGTCAGCT CATGGGAGAT 
CCTGCTTCAG GCAAAAGGAA AGATATGGAA GGACTGATCC CAGGAACCAA AACCTCTTCA 
TCCCAGCTGA ACAAATCACA AAGCCACATG GCTAAGGATA TTCCGTTCAA GACACAGGGT 
TCCCATGGAT CACAAGGGGC AGACTTTTCT GGAGATGAGA GTGAGGAAAG TAGTACCCAA 
GATACTTCCA AATGGAAAAG GACAGCAAAA TGTCACACGT CAAGCACACA GAGCCACTGC 
TACCAAACCA TCCACCCTGC TCATGAGCCT GAAGGCAAAG TGGACTTCCC GGAGCCTCTG 
GTAAAGGCCC ACCAGCAGGC TTACACCTAT CTACACTCCA GCCTCTCCAA ATATGAAGCA 
ATTCTGTGCA TCATCCATCA GGCCACCCAG ACCCGGGAGC TGCTGCAGCC CATGGTCAGC 
TTCTTGCTGC TGTGCTTTGA GGAGATCAGC CAGCTGTTGG GGGAGATCTC CAAGGATGGA 
GAAGTGCTCC TGCAGGAAGT CAGGGAGGAT CTGGCTTGGC CTTTGAAGAA AAGAGAGCCC 
CAGGAGCAGC CAAATCTCCT GCAACAGCTG CTACAGTACA CAGTCAGCAA GCTGCAGGTG 
CTCAATGGCA CAGTGGCCTC GCTCACCGGC AGCTTCCTGG AGGGCTCCAG CAGCTACCTC 
CACTCCACTG CAACCCACTT GGAAAATAAG CTGAGCACAA AAAGGAATGT GGATGAACGC 
CTCCTGAGGG CTCTGAGGCA GCTAGAGAGC CTGGCGAGTG GCTGTGGCGA CCCTGGGGTG 
CAGGGTCTCC CCTTATGCTC TGAGGACAGT GGCATTGGTG CTGACAATGA GTCCGTGCAG 
TCGGTGGACA AGCTGGGCAA GCAAACCAGC TGGGACCTTG CACCAGAGCC CGAAGAATGG 
AAGTCGGTGA CTTCACCCCA CACAGAGGCC AGGCAGTCAG GACACACCTG GCAGCAAAGT 
CCATTCTGTT TGGGCTCAGG CAGACCCCAG GACTGCCTGC TCTCAGGGGC TCCTATGGCA 
AAGGTTCAGC CACGAGCACA GGACGAAGCA AGGAGCCCAT GCCTCTCCAG TACAAGCCCA 
GAAAATATCA CCTCCCCACC TTTGAAGCTG GGGACAAGCA CCCCATGTGA TTCCTTTGGG 
ATTGGGGTCT CTGTGGAACC ACACCTTTCC AAAACCTCCA GGCCGATGGA CGCTTCATCT 
CTTAGTGACA GCGAGGACAG CAGCCCAGAG GAGGAGGAGG AAGACAAAAT GAGCAGCATG 
AGTCTGTGTG CCTGGCAGGA AAAAACTCCA CATTCAAGGC CACAATCTTC ACCTGCTGAC 
CGGGAAAGCC CATTTCAGGC CCGCACCAGG AGGCTTAGGA GCCTCCAGGC CCAGGAAATG 
ATTCTGAAGA TGAAGGAGTC AATCAGCGAA AGGATCAAGT TTGTCCCTGT GCCCTGTGGG 
CACCAGGACT GGTCTGAGGA GGAGGAGGGG AGGACAGTGG TCCCCCCAAG ACCTAGCACG 
GTAAGTGGCA GCAGGAGGGC CCCTGAGAGG CAGACGAGGT CCCAGTCAGA GTCGTGTCTC 
CAGAGTCACG TGGAGGACCC CACCTTTCAG GAGCTGCGAA GGGTCCAGAG GGACCTCAGT 
CAGAAGCTGG AGGCATTTTA TGCCCTGGGT GCCAAAGGGC AGGGGCAGAG CCAGGAGCAA 
ATTCTGCAGC CCAGAGCAGC CGCCGTGTGG CCCAATGGCA CCTGCAGGGT CAGTCCAAGC 
AACACCACCA GCAGGCTCAA GGCATCCCTC ACCAAGAACT TCAGTATTTT GCCTAGTCAG 
GACAAGAGCA TCTTGCAGAA ATGCAATCCC CATCCTGAGG ACGAACAAGG CAAAGCTGGG 
AAGCTTCCAA ATGCCATCCC ATCAGGAGAG GTCAGTGAGG CTGCCAAGGC CACAGACTGG 
AATGTCAGAG GCTGTCCCAC CAGAACATCC GTCAAGAAGC TTATTGAAAC TTTCAGTCCC 
ACGGAGAGTC TGAGGATGCT GGGGGACTCT AAGGACGCTG GGGCAAGTCC CTGCCTCAGG 
AATTGCATCA TGCCCCCCAG ATTTCCCAAG TACACAGGGC TTGCCCCTTT GTATCCGAAG 
CCCCAAATTT CTCCAGCATC AGGCAGAGAA TCTCTCAAAA TGGGCATAGG CTGGAAGCCC 
TTAGCACCTA TCTTTCCCCC TCTGCCTAAA GCAGAAGCAG CCAAGAGTGA GGAGCTCAGC 
TGTGAAATGG AGGGGAACCT CGAGCACCTC CCTCCACCGC CTATGGAAGT TCTGATGGAC 
AAATCATTCG CTTCTCTGGA GTCCCCAGAA AGCAGCAAGT CCACAGAGAA CTCCCCCAAG 
GAAACCCAGG AGCCAGGGCC GGGAGAGGCT GGCCCCACCA GGAGAACATG GGCTTCCCCA 
AAGCTGAGGG CCTCTGTGAG CCCCCTGGAC TTGCTGCCCA GCAAGAGCAC CGCCAGCCTG 
ACCAAGCCTC ACAGCACAGG GCCAGGGAGT GGCAGGAGCA GCTGCCAGCC CAGGAAGCCA 
GCCCTGGACC TGAGCAGCCC ACCAGCCACC AGCCAAAGCC CCGAGGTGAA GGGTGGGACT 
TGGAGTCAGG CAGAGAAGGC CACCAGCCTC TACAGGCAGC CCCGGAAGGC CATCGCCTGG 
CACCACTCCG GCCCTCCATC TGGACAAAAC AGGACCTCAG AGTCCAGCCT GGCCAGACCA 
AGGCAGAGCC GAGAGAGAAG CCCCCCTGTG GGCAGAAAGG CCTCTCCCAC GAGGACACAC 
TGGGTGCCTC AAGCAGACAA GAGGCGCCGG AGCCTTCCCT CCTCTTACAG ACCTGCCCAG 
CCAAGCCCCT CTGCTGTGCA GACGCCCCCC AGCCCACCTG TGAGCCCCAG GGTGCTAAGC 
CCACCCACCA CAAAGCGGCG AACTTCCCCA CCGCACCAGC CCAAGTTGCC CAACCCTCCC 
CCCGAGAGTG CACCTGCTCA GTGCAAGGTC CCCAGCCCCC CAACCCAGCA CCCAGAAGCA 
AGCCCCCCTT TCTCGATTCC CTCCCCATCA CCCCCAATGT CCCCTTCTCA GGAGCACAAG 
GAAACAAGAG ACTCTGAAGA CAGCCAAGCA GTCATAGCCA AAGTGTCTGG GAACACACAT 
TCCATATTCT GCCCAGCTAC CTCCTCTCTG TTTGAAGCTA AACCGCCACT CTCAACAGCC 
CACCCACTGA CCCCACCATC GCTGCCGCCA GAGGCTGGGG GCCCTCTCGG GAACCCAGCA 
GAATGCTGGA AGAACAGCTC AGGGCCTTGG CTGAGAGCAG ACTCGCAGCG GAGAGCAGCT 
CTGTGTGCCC TCAACCCTCT GCCTTTCCTC AGGAGGACAG CTTCTGACCG CCAGCCAGGT 
GGCCGACCGC AGCCTCCCAC CTTGGACCCC ACCAGCACCT CTTATGAATC CCAGCTCGGC 
CAGAACAGCA GCAGCGAGGA GAGCCCTAAG AAGGACACAG AGCCGGGGAG CAGCCCCTGT 
TCCCCTGAAC TGCAGGGCGG CACCAGGCGT GCATCTCCCC CAGAGTTCTG TGTGCTGGGC 
CACGGGCTGC AACCGGAGCC TCGGACCGGC CACATCCAGG ACAAATCCCA GCCAGAGGCG 
CAGCCCCAGC AAGAGGAGGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PCARE ( XM_011532826.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531128.1 Homo sapiens chromosome 2 open reading frame 71 (C2orf71), transcript variant X1, mRNA.

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CloneID	OHu21482
Clone ID Related Accession (Same CDS sequence)	NM_001029883.2 , XM_011532826.1 , NM_001029883.3
Accession Version	XM_011532826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	uncharacterized protein C2orf71 isoform X1
Comment

ATGGGGTGTA CACCTTCACA CAGTGACCTT GTAAACAGCG TTGCAAAGAG TGGCATTCAG 
TTCTTGAAAA AGCCCAAAGC AATTCGGCCA GGATGTCAGG GCGGAAGTGA AAGAGGTTCC 
ATCCCTTTGC TGGTTAAAAA CTCCACCTGC TATGACGCTG GGGAGGGCCT GGCAGAGGAG 
CAGCCAAGTC CCAGGAGGAA CCAAACCACA GCTAAAGGTC TTTGTCAGCT CATGGGAGAT 
CCTGCTTCAG GCAAAAGGAA AGATATGGAA GGACTGATCC CAGGAACCAA AACCTCTTCA 
TCCCAGCTGA ACAAATCACA AAGCCACATG GCTAAGGATA TTCCGTTCAA GACACAGGGT 
TCCCATGGAT CACAAGGGGC AGACTTTTCT GGAGATGAGA GTGAGGAAAG TAGTACCCAA 
GATACTTCCA AATGGAAAAG GACAGCAAAA TGTCACACGT CAAGCACACA GAGCCACTGC 
TACCAAACCA TCCACCCTGC TCATGAGCCT GAAGGCAAAG TGGACTTCCC GGAGCCTCTG 
GTAAAGGCCC ACCAGCAGGC TTACACCTAT CTACACTCCA GCCTCTCCAA ATATGAAGCA 
ATTCTGTGCA TCATCCATCA GGCCACCCAG ACCCGGGAGC TGCTGCAGCC CATGGTCAGC 
TTCTTGCTGC TGTGCTTTGA GGAGATCAGC CAGCTGTTGG GGGAGATCTC CAAGGATGGA 
GAAGTGCTCC TGCAGGAAGT CAGGGAGGAT CTGGCTTGGC CTTTGAAGAA AAGAGAGCCC 
CAGGAGCAGC CAAATCTCCT GCAACAGCTG CTACAGTACA CAGTCAGCAA GCTGCAGGTG 
CTCAATGGCA CAGTGGCCTC GCTCACCGGC AGCTTCCTGG AGGGCTCCAG CAGCTACCTC 
CACTCCACTG CAACCCACTT GGAAAATAAG CTGAGCACAA AAAGGAATGT GGATGAACGC 
CTCCTGAGGG CTCTGAGGCA GCTAGAGAGC CTGGCGAGTG GCTGTGGCGA CCCTGGGGTG 
CAGGGTCTCC CCTTATGCTC TGAGGACAGT GGCATTGGTG CTGACAATGA GTCCGTGCAG 
TCGGTGGACA AGCTGGGCAA GCAAACCAGC TGGGACCTTG CACCAGAGCC CGAAGAATGG 
AAGTCGGTGA CTTCACCCCA CACAGAGGCC AGGCAGTCAG GACACACCTG GCAGCAAAGT 
CCATTCTGTT TGGGCTCAGG CAGACCCCAG GACTGCCTGC TCTCAGGGGC TCCTATGGCA 
AAGGTTCAGC CACGAGCACA GGACGAAGCA AGGAGCCCAT GCCTCTCCAG TACAAGCCCA 
GAAAATATCA CCTCCCCACC TTTGAAGCTG GGGACAAGCA CCCCATGTGA TTCCTTTGGG 
ATTGGGGTCT CTGTGGAACC ACACCTTTCC AAAACCTCCA GGCCGATGGA CGCTTCATCT 
CTTAGTGACA GCGAGGACAG CAGCCCAGAG GAGGAGGAGG AAGACAAAAT GAGCAGCATG 
AGTCTGTGTG CCTGGCAGGA AAAAACTCCA CATTCAAGGC CACAATCTTC ACCTGCTGAC 
CGGGAAAGCC CATTTCAGGC CCGCACCAGG AGGCTTAGGA GCCTCCAGGC CCAGGAAATG 
ATTCTGAAGA TGAAGGAGTC AATCAGCGAA AGGATCAAGT TTGTCCCTGT GCCCTGTGGG 
CACCAGGACT GGTCTGAGGA GGAGGAGGGG AGGACAGTGG TCCCCCCAAG ACCTAGCACG 
GTAAGTGGCA GCAGGAGGGC CCCTGAGAGG CAGACGAGGT CCCAGTCAGA GTCGTGTCTC 
CAGAGTCACG TGGAGGACCC CACCTTTCAG GAGCTGCGAA GGGTCCAGAG GGACCTCAGT 
CAGAAGCTGG AGGCATTTTA TGCCCTGGGT GCCAAAGGGC AGGGGCAGAG CCAGGAGCAA 
ATTCTGCAGC CCAGAGCAGC CGCCGTGTGG CCCAATGGCA CCTGCAGGGT CAGTCCAAGC 
AACACCACCA GCAGGCTCAA GGCATCCCTC ACCAAGAACT TCAGTATTTT GCCTAGTCAG 
GACAAGAGCA TCTTGCAGAA ATGCAATCCC CATCCTGAGG ACGAACAAGG CAAAGCTGGG 
AAGCTTCCAA ATGCCATCCC ATCAGGAGAG GTCAGTGAGG CTGCCAAGGC CACAGACTGG 
AATGTCAGAG GCTGTCCCAC CAGAACATCC GTCAAGAAGC TTATTGAAAC TTTCAGTCCC 
ACGGAGAGTC TGAGGATGCT GGGGGACTCT AAGGACGCTG GGGCAAGTCC CTGCCTCAGG 
AATTGCATCA TGCCCCCCAG ATTTCCCAAG TACACAGGGC TTGCCCCTTT GTATCCGAAG 
CCCCAAATTT CTCCAGCATC AGGCAGAGAA TCTCTCAAAA TGGGCATAGG CTGGAAGCCC 
TTAGCACCTA TCTTTCCCCC TCTGCCTAAA GCAGAAGCAG CCAAGAGTGA GGAGCTCAGC 
TGTGAAATGG AGGGGAACCT CGAGCACCTC CCTCCACCGC CTATGGAAGT TCTGATGGAC 
AAATCATTCG CTTCTCTGGA GTCCCCAGAA AGCAGCAAGT CCACAGAGAA CTCCCCCAAG 
GAAACCCAGG AGCCAGGGCC GGGAGAGGCT GGCCCCACCA GGAGAACATG GGCTTCCCCA 
AAGCTGAGGG CCTCTGTGAG CCCCCTGGAC TTGCTGCCCA GCAAGAGCAC CGCCAGCCTG 
ACCAAGCCTC ACAGCACAGG GCCAGGGAGT GGCAGGAGCA GCTGCCAGCC CAGGAAGCCA 
GCCCTGGACC TGAGCAGCCC ACCAGCCACC AGCCAAAGCC CCGAGGTGAA GGGTGGGACT 
TGGAGTCAGG CAGAGAAGGC CACCAGCCTC TACAGGCAGC CCCGGAAGGC CATCGCCTGG 
CACCACTCCG GCCCTCCATC TGGACAAAAC AGGACCTCAG AGTCCAGCCT GGCCAGACCA 
AGGCAGAGCC GAGAGAGAAG CCCCCCTGTG GGCAGAAAGG CCTCTCCCAC GAGGACACAC 
TGGGTGCCTC AAGCAGACAA GAGGCGCCGG AGCCTTCCCT CCTCTTACAG ACCTGCCCAG 
CCAAGCCCCT CTGCTGTGCA GACGCCCCCC AGCCCACCTG TGAGCCCCAG GGTGCTAAGC 
CCACCCACCA CAAAGCGGCG AACTTCCCCA CCGCACCAGC CCAAGTTGCC CAACCCTCCC 
CCCGAGAGTG CACCTGCTCA GTGCAAGGTC CCCAGCCCCC CAACCCAGCA CCCAGAAGCA 
AGCCCCCCTT TCTCGATTCC CTCCCCATCA CCCCCAATGT CCCCTTCTCA GGAGCACAAG 
GAAACAAGAG ACTCTGAAGA CAGCCAAGCA GTCATAGCCA AAGTGTCTGG GAACACACAT 
TCCATATTCT GCCCAGCTAC CTCCTCTCTG TTTGAAGCTA AACCGCCACT CTCAACAGCC 
CACCCACTGA CCCCACCATC GCTGCCGCCA GAGGCTGGGG GCCCTCTCGG GAACCCAGCA 
GAATGCTGGA AGAACAGCTC AGGGCCTTGG CTGAGAGCAG ACTCGCAGCG GAGAGCAGCT 
CTGTGTGCCC TCAACCCTCT GCCTTTCCTC AGGAGGACAG CTTCTGACCG CCAGCCAGGT 
GGCCGACCGC AGCCTCCCAC CTTGGACCCC ACCAGCACCT CTTATGAATC CCAGCTCGGC 
CAGAACAGCA GCAGCGAGGA GAGCCCTAAG AAGGACACAG AGCCGGGGAG CAGCCCCTGT 
TCCCCTGAAC TGCAGGGCGG CACCAGGCGT GCATCTCCCC CAGAGTTCTG TGTGCTGGGC 
CACGGGCTGC AACCGGAGCC TCGGACCGGC CACATCCAGG ACAAATCCCA GCCAGAGGCG 
CAGCCCCAGC AAGAGGAGGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011531128.1

Translation

MGCTPSHSDLVNSVAKSGIQFLKKPKAIRPGCQGGSERGSIPLLVKNSTCYDAGEGLAEEQPSPRRNQTTAKGLCQLMGDPASGKRKDMEGLIPGTKTSSSQLNKSQSHMAKDIPFKTQGSHGSQGADFSGDESEESSTQDTSKWKRTAKCHTSSTQSHCYQTIHPAHEPEGKVDFPEPLVKAHQQAYTYLHSSLSKYEAILCIIHQATQTRELLQPMVSFLLLCFEEISQLLGEISKDGEVLLQEVREDLAWPLKKREPQEQPNLLQQLLQYTVSKLQVLNGTVASLTGSFLEGSSSYLHSTATHLENKLSTKRNVDERLLRALRQLESLASGCGDPGVQGLPLCSEDSGIGADNESVQSVDKLGKQTSWDLAPEPEEWKSVTSPHTEARQSGHTWQQSPFCLGSGRPQDCLLSGAPMAKVQPRAQDEARSPCLSSTSPENITSPPLKLGTSTPCDSFGIGVSVEPHLSKTSRPMDASSLSDSEDSSPEEEEEDKMSSMSLCAWQEKTPHSRPQSSPADRESPFQARTRRLRSLQAQEMILKMKESISERIKFVPVPCGHQDWSEEEEGRTVVPPRPSTVSGSRRAPERQTRSQSESCLQSHVEDPTFQELRRVQRDLSQKLEAFYALGAKGQGQSQEQILQPRAAAVWPNGTCRVSPSNTTSRLKASLTKNFSILPSQDKSILQKCNPHPEDEQGKAGKLPNAIPSGEVSEAAKATDWNVRGCPTRTSVKKLIETFSPTESLRMLGDSKDAGASPCLRNCIMPPRFPKYTGLAPLYPKPQISPASGRESLKMGIGWKPLAPIFPPLPKAEAAKSEELSCEMEGNLEHLPPPPMEVLMDKSFASLESPESSKSTENSPKETQEPGPGEAGPTRRTWASPKLRASVSPLDLLPSKSTASLTKPHSTGPGSGRSSCQPRKPALDLSSPPATSQSPEVKGGTWSQAEKATSLYRQPRKAIAWHHSGPPSGQNRTSESSLARPRQSRERSPPVGRKASPTRTHWVPQADKRRRSLPSSYRPAQPSPSAVQTPPSPPVSPRVLSPPTTKRRTSPPHQPKLPNPPPESAPAQCKVPSPPTQHPEASPPFSIPSPSPPMSPSQEHKETRDSEDSQAVIAKVSGNTHSIFCPATSSLFEAKPPLSTAHPLTPPSLPPEAGGPLGNPAECWKNSSGPWLRADSQRRAALCALNPLPFLRRTASDRQPGGRPQPPTLDPTSTSYESQLGQNSSSEESPKKDTEPGSSPCSPELQGGTRRASPPEFCVLGHGLQPEPRTGHIQDKSQPEAQPQQEEVS

Target ORF information:

RefSeq Version	XM_011532826.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens chromosome 2 open reading frame 71 (C2orf71), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011532826.1

ORF Insert Sequence:

ATGGGGTGTA CACCTTCACA CAGTGACCTT GTAAACAGCG TTGCAAAGAG TGGCATTCAG 
TTCTTGAAAA AGCCCAAAGC AATTCGGCCA GGATGTCAGG GCGGAAGTGA AAGAGGTTCC 
ATCCCTTTGC TGGTTAAAAA CTCCACCTGC TATGACGCTG GGGAGGGCCT GGCAGAGGAG 
CAGCCAAGTC CCAGGAGGAA CCAAACCACA GCTAAAGGTC TTTGTCAGCT CATGGGAGAT 
CCTGCTTCAG GCAAAAGGAA AGATATGGAA GGACTGATCC CAGGAACCAA AACCTCTTCA 
TCCCAGCTGA ACAAATCACA AAGCCACATG GCTAAGGATA TTCCGTTCAA GACACAGGGT 
TCCCATGGAT CACAAGGGGC AGACTTTTCT GGAGATGAGA GTGAGGAAAG TAGTACCCAA 
GATACTTCCA AATGGAAAAG GACAGCAAAA TGTCACACGT CAAGCACACA GAGCCACTGC 
TACCAAACCA TCCACCCTGC TCATGAGCCT GAAGGCAAAG TGGACTTCCC GGAGCCTCTG 
GTAAAGGCCC ACCAGCAGGC TTACACCTAT CTACACTCCA GCCTCTCCAA ATATGAAGCA 
ATTCTGTGCA TCATCCATCA GGCCACCCAG ACCCGGGAGC TGCTGCAGCC CATGGTCAGC 
TTCTTGCTGC TGTGCTTTGA GGAGATCAGC CAGCTGTTGG GGGAGATCTC CAAGGATGGA 
GAAGTGCTCC TGCAGGAAGT CAGGGAGGAT CTGGCTTGGC CTTTGAAGAA AAGAGAGCCC 
CAGGAGCAGC CAAATCTCCT GCAACAGCTG CTACAGTACA CAGTCAGCAA GCTGCAGGTG 
CTCAATGGCA CAGTGGCCTC GCTCACCGGC AGCTTCCTGG AGGGCTCCAG CAGCTACCTC 
CACTCCACTG CAACCCACTT GGAAAATAAG CTGAGCACAA AAAGGAATGT GGATGAACGC 
CTCCTGAGGG CTCTGAGGCA GCTAGAGAGC CTGGCGAGTG GCTGTGGCGA CCCTGGGGTG 
CAGGGTCTCC CCTTATGCTC TGAGGACAGT GGCATTGGTG CTGACAATGA GTCCGTGCAG 
TCGGTGGACA AGCTGGGCAA GCAAACCAGC TGGGACCTTG CACCAGAGCC CGAAGAATGG 
AAGTCGGTGA CTTCACCCCA CACAGAGGCC AGGCAGTCAG GACACACCTG GCAGCAAAGT 
CCATTCTGTT TGGGCTCAGG CAGACCCCAG GACTGCCTGC TCTCAGGGGC TCCTATGGCA 
AAGGTTCAGC CACGAGCACA GGACGAAGCA AGGAGCCCAT GCCTCTCCAG TACAAGCCCA 
GAAAATATCA CCTCCCCACC TTTGAAGCTG GGGACAAGCA CCCCATGTGA TTCCTTTGGG 
ATTGGGGTCT CTGTGGAACC ACACCTTTCC AAAACCTCCA GGCCGATGGA CGCTTCATCT 
CTTAGTGACA GCGAGGACAG CAGCCCAGAG GAGGAGGAGG AAGACAAAAT GAGCAGCATG 
AGTCTGTGTG CCTGGCAGGA AAAAACTCCA CATTCAAGGC CACAATCTTC ACCTGCTGAC 
CGGGAAAGCC CATTTCAGGC CCGCACCAGG AGGCTTAGGA GCCTCCAGGC CCAGGAAATG 
ATTCTGAAGA TGAAGGAGTC AATCAGCGAA AGGATCAAGT TTGTCCCTGT GCCCTGTGGG 
CACCAGGACT GGTCTGAGGA GGAGGAGGGG AGGACAGTGG TCCCCCCAAG ACCTAGCACG 
GTAAGTGGCA GCAGGAGGGC CCCTGAGAGG CAGACGAGGT CCCAGTCAGA GTCGTGTCTC 
CAGAGTCACG TGGAGGACCC CACCTTTCAG GAGCTGCGAA GGGTCCAGAG GGACCTCAGT 
CAGAAGCTGG AGGCATTTTA TGCCCTGGGT GCCAAAGGGC AGGGGCAGAG CCAGGAGCAA 
ATTCTGCAGC CCAGAGCAGC CGCCGTGTGG CCCAATGGCA CCTGCAGGGT CAGTCCAAGC 
AACACCACCA GCAGGCTCAA GGCATCCCTC ACCAAGAACT TCAGTATTTT GCCTAGTCAG 
GACAAGAGCA TCTTGCAGAA ATGCAATCCC CATCCTGAGG ACGAACAAGG CAAAGCTGGG 
AAGCTTCCAA ATGCCATCCC ATCAGGAGAG GTCAGTGAGG CTGCCAAGGC CACAGACTGG 
AATGTCAGAG GCTGTCCCAC CAGAACATCC GTCAAGAAGC TTATTGAAAC TTTCAGTCCC 
ACGGAGAGTC TGAGGATGCT GGGGGACTCT AAGGACGCTG GGGCAAGTCC CTGCCTCAGG 
AATTGCATCA TGCCCCCCAG ATTTCCCAAG TACACAGGGC TTGCCCCTTT GTATCCGAAG 
CCCCAAATTT CTCCAGCATC AGGCAGAGAA TCTCTCAAAA TGGGCATAGG CTGGAAGCCC 
TTAGCACCTA TCTTTCCCCC TCTGCCTAAA GCAGAAGCAG CCAAGAGTGA GGAGCTCAGC 
TGTGAAATGG AGGGGAACCT CGAGCACCTC CCTCCACCGC CTATGGAAGT TCTGATGGAC 
AAATCATTCG CTTCTCTGGA GTCCCCAGAA AGCAGCAAGT CCACAGAGAA CTCCCCCAAG 
GAAACCCAGG AGCCAGGGCC GGGAGAGGCT GGCCCCACCA GGAGAACATG GGCTTCCCCA 
AAGCTGAGGG CCTCTGTGAG CCCCCTGGAC TTGCTGCCCA GCAAGAGCAC CGCCAGCCTG 
ACCAAGCCTC ACAGCACAGG GCCAGGGAGT GGCAGGAGCA GCTGCCAGCC CAGGAAGCCA 
GCCCTGGACC TGAGCAGCCC ACCAGCCACC AGCCAAAGCC CCGAGGTGAA GGGTGGGACT 
TGGAGTCAGG CAGAGAAGGC CACCAGCCTC TACAGGCAGC CCCGGAAGGC CATCGCCTGG 
CACCACTCCG GCCCTCCATC TGGACAAAAC AGGACCTCAG AGTCCAGCCT GGCCAGACCA 
AGGCAGAGCC GAGAGAGAAG CCCCCCTGTG GGCAGAAAGG CCTCTCCCAC GAGGACACAC 
TGGGTGCCTC AAGCAGACAA GAGGCGCCGG AGCCTTCCCT CCTCTTACAG ACCTGCCCAG 
CCAAGCCCCT CTGCTGTGCA GACGCCCCCC AGCCCACCTG TGAGCCCCAG GGTGCTAAGC 
CCACCCACCA CAAAGCGGCG AACTTCCCCA CCGCACCAGC CCAAGTTGCC CAACCCTCCC 
CCCGAGAGTG CACCTGCTCA GTGCAAGGTC CCCAGCCCCC CAACCCAGCA CCCAGAAGCA 
AGCCCCCCTT TCTCGATTCC CTCCCCATCA CCCCCAATGT CCCCTTCTCA GGAGCACAAG 
GAAACAAGAG ACTCTGAAGA CAGCCAAGCA GTCATAGCCA AAGTGTCTGG GAACACACAT 
TCCATATTCT GCCCAGCTAC CTCCTCTCTG TTTGAAGCTA AACCGCCACT CTCAACAGCC 
CACCCACTGA CCCCACCATC GCTGCCGCCA GAGGCTGGGG GCCCTCTCGG GAACCCAGCA 
GAATGCTGGA AGAACAGCTC AGGGCCTTGG CTGAGAGCAG ACTCGCAGCG GAGAGCAGCT 
CTGTGTGCCC TCAACCCTCT GCCTTTCCTC AGGAGGACAG CTTCTGACCG CCAGCCAGGT 
GGCCGACCGC AGCCTCCCAC CTTGGACCCC ACCAGCACCT CTTATGAATC CCAGCTCGGC 
CAGAACAGCA GCAGCGAGGA GAGCCCTAAG AAGGACACAG AGCCGGGGAG CAGCCCCTGT 
TCCCCTGAAC TGCAGGGCGG CACCAGGCGT GCATCTCCCC CAGAGTTCTG TGTGCTGGGC 
CACGGGCTGC AACCGGAGCC TCGGACCGGC CACATCCAGG ACAAATCCCA GCCAGAGGCG 
CAGCCCCAGC AAGAGGAGGT GTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

C2orf71 Mutations as a Frequent Cause of Autosomal-Recessive Retinitis Pigmentosa: Clinical Analysis and Presentation of 8 Novel Mutations.
Investigative ophthalmology & visual science58(10)3840-3850(2017 Aug)
Gerth-Kahlert C,Tiwari A,Hanson JVM,Batmanabane V,Traboulsi E,Pennesi ME,Al-Qahtani AA,Lam BL,Heckenlively J,Zweifel SA,Vincent A,Fierz F,Barthelmes D,Branham K,Khan N,Bahr A,Baehr L,Magyar I,Koller S,Azzarello-Burri S,Niedrist D,Heon E,Berger W

Putative digenic inheritance of heterozygous RP1L1 and C2orf71 null mutations in syndromic retinal dystrophy.
Ophthalmic genetics38(2)127-132(2017 Mar-Apr)
Liu YP,Bosch DG,Siemiatkowska AM,Rendtorff ND,Boonstra FN,M?ller C,Tranebj?rg L,Katsanis N,Cremers FP

A homozygous nonsense CEP250 mutation combined with a heterozygous nonsense C2orf71 mutation is associated with atypical Usher syndrome.
Journal of medical genetics51(7)460-9(2014 Jul)
Khateb S,Zelinger L,Mizrahi-Meissonnier L,Ayuso C,Koenekoop RK,Laxer U,Gross M,Banin E,Sharon D

Novel C2orf71 mutations account for ?1% of cases in a large French arRP cohort.
Human mutation32(4)E2091-103(2011 Apr)
Audo I,Lancelot ME,Mohand-Sa?d S,Antonio A,Germain A,Sahel JA,Bhattacharya SS,Zeitz C

A survey of DNA variation of C2ORF71 in probands with progressive autosomal recessive retinal degeneration and controls.
Investigative ophthalmology & visual science52(3)1880-6(2011 Mar)
Sergouniotis PI,Li Z,Mackay DS,Wright GA,Borman AD,Devery SR,Moore AT,Webster AR

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PCARE cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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