SMIM1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SMIM1
Entrez Gene ID	388588
Full Name	small integral membrane protein 1 (Vel blood group)
Synonyms	Vel
General protein information	Preferred Names small integral membrane protein 1 (Vel blood group) Names small integral membrane protein 1 vel blood group antigen
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.32
Summary	This gene encodes a small, conserved protein that participates in red blood cell formation. The encoded protein is localized to the cell membrane and is the antigen for the Vel blood group. Alternative splicing results in different transcript variants that encode the same protein. [provided by RefSeq, Dec 2013].
Disorder MIM:	615242
Disorder Html:	[Blood group, Vel system], 615264 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001288583.1	NP_001275512.1	small integral membrane protein 1
NM_001163724.2	NP_001157196.1	small integral membrane protein 1

Pathways from BioSystems

Homologies

	SMIM1	XP_001084487.1
Bos taurus (cattle)	SMIM1	NP_001157199.1
Canis lupus familiaris (dog)	SMIM1	XP_003639106.1
Xenopus tropicalis (tropical clawed frog)	smim1	XP_002937501.1
Homo sapiens (human)	SMIM1	NP_001157196.1
Pan troglodytes (chimpanzee)	SMIM1	XP_003949327.1
Mus musculus (house mouse)	Smim1	NP_001157194.1
Rattus norvegicus (Norway rat)	Smim1	NP_001157195.1

Gene Ontology
Bibliography

Related articles in PubMed

SMIM1 variants rs1175550 and rs143702418 independently modulate Vel blood group antigen expression.
Christophersen MK, J?ud M, Ajore R, Vege S, Ljungdahl KW, Westhoff CM, Olsson ML, Storry JR, Nilsson B
Scientific reports740451(2017 Jan)

Screening for the SMIM1*64_80 del Allele in blood donors in a population from Southern Brazil.
Costa DC, Dezan M, Santos T, Schinaider AA, Sch?rner EJ, Levi JE, Santos-Silva MC
Transfusion medicine (Oxford, England)26(5)355-359(2016 Oct)

The c.64_80del SMIM1 allele is segregating in the Hutterite population.
Coghlan G, Zelinski T
Transfusion56(4)946-9(2016 Apr)

SMIM1 is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus.
Arnaud L, Kelley LP, Helias V, Cartron JP, Ballif BA
FEBS letters589(23)3624-30(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Among the 448 samples analysed, 10 (2.23%) harboured the 17 bp deletion of the gene SMIM1, and all were heterozygote for the SMIM1*64_80 del allele.
Title: Screening for the SMIM1*64_80 del Allele in blood donors in a population from Southern Brazil.

Alleles that initially entered the population have been maintained within the population. The c.64_80del null allele of SMIM1 is one such allele, thus having implications for transfusion medicine and child or maternal health.
Title: The c.64_80del SMIM1 allele is segregating in the Hutterite population.

SMIM1 carries the Vel antigen as a type II membrane protein with a predicted C-terminal extracellular domain of only 3-12 amino acids
Title: SMIM1 is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus.

Weak Vel expression levels are caused by multiple genetic factors in SMIM1 and probably also by other genetic or environmental factors.
Title: Impact of genetic variation in the SMIM1 gene on Vel expression levels.

Loss of SMIM1 is associated with reduction in red blood cell formation.
Title: SMIM1 underlies the Vel blood group and influences red blood cell traits.

The following SMIM1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMIM1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu14378	NM_001288583.1 Latest version!	Homo sapiens small integral membrane protein 1 (Vel blood group) (SMIM1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14378	NM_001163724.2 Latest version!	Homo sapiens small integral membrane protein 1 (Vel blood group) (SMIM1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMIM1 ( NM_001288583.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001275512.1 Homo sapiens small integral membrane protein 1 (Vel blood group) (SMIM1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14378
Clone ID Related Accession (Same CDS sequence)	NM_001288583.1 , NM_001163724.2
Accession Version	NM_001288583.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	small integral membrane protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY032469.1, KC152644.1 and AI990490.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: HY032469.1, KC152644.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCCC AGGAGAGCCA CGTCCACTAT AGTAGGTGGG AGGACGGCAG CAGGGACGGA 
GTCAGCCTAG GGGCTGTGTC CAGCACAGAA GAGGCCTCAC GCTGCCGCAG GATCTCCCAG 
AGGCTGTGCA CGGGCAAGCT GGGCATCGCC ATGAAGGTGC TGGGCGGCGT GGCCCTCTTC 
TGGATCATCT TCATCCTGGG CTACCTCACA GGCTACTATG TGCACAAGTG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275512.1
CDS	223..459
Translation	MQPQESHVHYSRWEDGSRDGVSLGAVSSTEEASRCRRISQRLCTGKLGIAMKVLGGVALFWIIFILGYLTGYYVHKCK

Target ORF information:

RefSeq Version	NM_001288583.1
Organism	Homo sapiens(human)
Definition	Homo sapiens small integral membrane protein 1 (Vel blood group) (SMIM1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288583.1

ORF Insert Sequence:

ATGCAGCCCC AGGAGAGCCA CGTCCACTAT AGTAGGTGGG AGGACGGCAG CAGGGACGGA 
GTCAGCCTAG GGGCTGTGTC CAGCACAGAA GAGGCCTCAC GCTGCCGCAG GATCTCCCAG 
AGGCTGTGCA CGGGCAAGCT GGGCATCGCC ATGAAGGTGC TGGGCGGCGT GGCCCTCTTC 
TGGATCATCT TCATCCTGGG CTACCTCACA GGCTACTATG TGCACAAGTG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SMIM1 ( NM_001163724.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157196.1 Homo sapiens small integral membrane protein 1 (Vel blood group) (SMIM1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14378
Clone ID Related Accession (Same CDS sequence)	NM_001288583.1 , NM_001163724.2
Accession Version	NM_001163724.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 237bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Homo sapiens(human)
Product	small integral membrane protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY003308.1 and AI990490.1. This sequence is a reference standard in the RefSeqGene project. On Dec 21, 2013 this sequence version replaced NM_001163724.1. Transcript Variant: This variant (1) encodes the longer transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: HY003308.1, BC042129.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAGCCCC AGGAGAGCCA CGTCCACTAT AGTAGGTGGG AGGACGGCAG CAGGGACGGA 
GTCAGCCTAG GGGCTGTGTC CAGCACAGAA GAGGCCTCAC GCTGCCGCAG GATCTCCCAG 
AGGCTGTGCA CGGGCAAGCT GGGCATCGCC ATGAAGGTGC TGGGCGGCGT GGCCCTCTTC 
TGGATCATCT TCATCCTGGG CTACCTCACA GGCTACTATG TGCACAAGTG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157196.1
CDS	250..486
Translation	MQPQESHVHYSRWEDGSRDGVSLGAVSSTEEASRCRRISQRLCTGKLGIAMKVLGGVALFWIIFILGYLTGYYVHKCK

Target ORF information:

RefSeq Version	NM_001163724.2
Organism	Homo sapiens(human)
Definition	Homo sapiens small integral membrane protein 1 (Vel blood group) (SMIM1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163724.2

ORF Insert Sequence:

ATGCAGCCCC AGGAGAGCCA CGTCCACTAT AGTAGGTGGG AGGACGGCAG CAGGGACGGA 
GTCAGCCTAG GGGCTGTGTC CAGCACAGAA GAGGCCTCAC GCTGCCGCAG GATCTCCCAG 
AGGCTGTGCA CGGGCAAGCT GGGCATCGCC ATGAAGGTGC TGGGCGGCGT GGCCCTCTTC 
TGGATCATCT TCATCCTGGG CTACCTCACA GGCTACTATG TGCACAAGTG CAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SMIM1 variants rs1175550 and rs143702418 independently modulate Vel blood group antigen expression.
Scientific reports740451(2017 Jan)
Christophersen MK,J?ud M,Ajore R,Vege S,Ljungdahl KW,Westhoff CM,Olsson ML,Storry JR,Nilsson B

Screening for the SMIM1*64_80 del Allele in blood donors in a population from Southern Brazil.
Transfusion medicine (Oxford, England)26(5)355-359(2016 Oct)
Costa DC,Dezan M,Santos T,Schinaider AA,Sch?rner EJ,Levi JE,Santos-Silva MC

The c.64_80del SMIM1 allele is segregating in the Hutterite population.
Transfusion56(4)946-9(2016 Apr)
Coghlan G,Zelinski T

SMIM1 is a type II transmembrane phosphoprotein and displays the Vel blood group antigen at its carboxyl-terminus.
FEBS letters589(23)3624-30(2015 Nov)
Arnaud L,Kelley LP,Helias V,Cartron JP,Ballif BA

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SMIM1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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