KRT3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KRT3
Entrez Gene ID	3850
Full Name	keratin 3
Synonyms	CK3,K3
General protein information	Preferred Names keratin 3 Names keratin, type II cytoskeletal 3 65 kDa cytokeratin CK-3 cytokeratin 3 keratin 3, type II type-II keratin Kb3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q13.13
Summary	The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the corneal epithelium with family member KRT12 and mutations in these genes have been associated with Meesmann's Corneal Dystrophy. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008].
Disorder MIM:	148043
Disorder Html:	Meesmann corneal dystrophy, 122100 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024448975.1	XP_024304743.1	keratin, type II cytoskeletal 3 isoform X1
NM_057088.2	NP_476429.2	keratin, type II cytoskeletal 3
NM_057088.3	NP_476429.2	keratin, type II cytoskeletal 3

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Human aniridia limbal epithelial cells lack expression of keratins K3 and K12.
Latta L, Viestenz A, Stachon T, Colanesi S, Szentm?ry N, Seitz B, K?smann-Kellner B
Experimental eye research167100-109(2017 Nov)

PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.
Sasamoto Y, Hayashi R, Park SJ, Saito-Adachi M, Suzuki Y, Kawasaki S, Quantock AJ, Nakai K, Tsujikawa M, Nishida K
Scientific reports620807(2016 Feb)

Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family.
Cao W, Yan M, Hao Q, Wang S, Wu L, Liu Q, Li M, Biddle FG, Wu W
The Journal of international medical research41(2)511-8(2013 Apr)

Genetics of Meesmann corneal dystrophy: a novel mutation in the keratin 3 gene in an asymptomatic family suggests genotype-phenotype correlation.
Szaflik JP, O?dak M, Maksym RB, Kami?ska A, Pollak A, Udziela M, P?oski R, Szaflik J
Molecular vision141713-8(2008 Sep)

New consensus nomenclature for mammalian keratins.
Schweizer J, Bowden PE, Coulombe PA, Langbein L, Lane EB, Magin TM, Maltais L, Omary MB, Parry DA, Rogers MA, Wright MW
The Journal of cell biology174(2)169-74(2006 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4, and OCT4 induced KRT12 expression
Title: PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.

Exon sequencing of KRT3 and KRT12 in six affected and eight unaffected individuals did not detect any mutations or nucleotide sequence variants in Meesmann epithelial corneal dystrophy.
Title: Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family.

Location of the E498V mutation emphasizes the functional relevance of the highly conserved boundary motifs at the COOH-terminus of the alpha-helical rod domain in keratin 3 (K3).
Title: Genetics of Meesmann corneal dystrophy: a novel mutation in the keratin 3 gene in an asymptomatic family suggests genotype-phenotype correlation.

A novel missense mutation (R503P) in KRT3 leads to MCD in 2 unrelated Taiwanese families.
Title: Novel mutations in the helix termination motif of keratin 3 and keratin 12 in 2 Taiwanese families with Meesmann corneal dystrophy.

The following KRT3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KRT3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu103724	XM_024448975.1 Latest version!	Homo sapiens keratin 3 (KRT3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu17842	NM_057088.3 Latest version!	Homo sapiens keratin 3 (KRT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu17842	NM_057088.2	Homo sapiens keratin 3 (KRT3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KRT3 ( XM_024448975.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024304743.1 Homo sapiens keratin 3 (KRT3), transcript variant X1, mRNA.

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CloneID	OHu103724
Clone ID Related Accession (Same CDS sequence)	XM_024448975.1
Accession Version	XM_024448975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	keratin, type II cytoskeletal 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000012.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGAATG CACTCTCTGC CTTGGCCACC CTACTGTGCC TCCCACCCTT GACTGCCGCA 
GAGTTCATTC AGACTCCAGT TCCTCCAGCC CTGTGTCCAT GGGGCCTCAT GGTGTGCAGG 
CAGAAAGGCA CGTCGTTTGC CCAAGACCAG TCCTTGCCCA TGGGAGTCTC GTGCAGATCC 
AAGGTCTTCA TTTCCCATCC CTCTTCCTCT GACTCTCAGT GTCCCTGCTT AACTTACACT 
CTTCTCTTCG CCAAGCTCCT TACCATGAGC AGACAAGCCA GCAAGACATC TGGTGGCGGG 
AGCCAGGGTT TCTCCGGCCG CTCTGCTGTG GTCTCCGGCA GCAGCAGGAT GAGCTGTGTG 
GCCCACTCTG GGGGAGCTGG CGGAGGGGCC TATGGCTTCC GGAGCGGAGC AGGTGGCTTT 
GGCAGTCGCA GCCTCTACAA CCTGGGCGGC AACAAGAGCA TCTCCATCAG CGTGGCAGCT 
GGCGGCTCCC GGGCTGGAGG CTTTGGGGGA GGGCGGAGCA GCTGTGCCTT TGCAGGTGGC 
TATGGAGGTG GCTTTGGGAG CGGCTATGGA GGTGGCTTTG GTGGTGGCTT TGGTGGTGGC 
AGAGGAATGG GAGGTGGCTT TGGTGGAGCT GGTGGCTTTG GAGGGGCTGG TGGCTTTGGA 
GGGGCTGGTG GCTTTGGTGG TCCTGGTGGC TTTGGTGGGT CTGGTGGCTT TGGTGGGCCT 
GGCAGCTTGG GCAGTCCTGG TGGCTTTGGC CCTGGGGGCT TTCCTGGGGG AATTCAGGAA 
GTGACTATCA ACCAGAGTCT CCTGCAGCCC CTCAATGTGG AGATCGACCC CCAGATTGGG 
CAAGTAAAGG CCCAGGAGCG GGAACAGATC AAGACCCTCA ACAACAAGTT TGCCTCCTTC 
ATTGACAAGG TGCGGTTCCT GGAGCAACAG AACAAAGTCC TGGAGACCAA GTGGAACCTG 
CTCCAGCAGC AGGGCACAAG TTCCATCTCA GGCACAAACA ACCTTGAGCC TCTTTTTGAG 
AATCACATCA ACTACCTGCG GAGCTACCTG GACAACATCC TCGGGGAGAG AGGGCGCCTG 
GACTCTGAGC TGAAGAACAT GGAGGACCTG GTGGAAGACT TCAAGAAGAA ATATGAGGAT 
GAAATCAATA AACGTACAGC TGCTGAGAAT GAATTTGTGA CTCTGAAGAA GGATGTGGAC 
AGTGCCTATA TGAACAAGGT GGAGCTTCAG GCCAAAGTGG ATGCCTTGAT AGATGAGATC 
GACTTCTTAA GGACCCTCTA CGACGCTGAG CTATCTCAGA TGCAGAGCCA CATCAGTGAC 
ACATCTGTGG TGCTGTCCAT GGACAATAAT CGCTCCCTGG ACCTGGACAG CATCATTGCT 
GAAGTTCGTG CACAGTATGA GGATATCGCT CAGAGAAGCA AGGCCGAAGC TGAGGCCCTG 
TACCAGACCA AGTTGGGGGA GCTGCAGACC ACGGCTGGCA GGCATGGGGA TGACCTAAGA 
AATACCAAGA GCGAGATCAT AGAGCTCAAC AGAATGATCC AGAGGCTGCG GGCAGAGATC 
GAGGGTGTCA AGAAGCAGAA TGCCAACCTG CAGACGGCCA TTGCCGAGGC CGAGCAGCAT 
GGAGAGATGG CCCTCAAGGA TGCCAATGCC AAGCTCCAAG AGCTGCAGGC TGCTCTACAG 
CAGGCGAAGG ATGACCTGGC GCGGCTGCTA CGTGACTACC AGGAGCTGAT GAATGTCAAG 
CTGGCCCTGG ACGTGGAGAT CGCCACCTAC CGCAAGCTGC TGGAGGGCGA GGAGTACAGG 
ATGTCTGGAG AGTGTCCGAG TGCTGTCAGC ATCTCCGTGG TCAGCAGCAG CACGACTTCC 
GCCTCCGCAG GTGGCTATGG AGGAGGTTAC GGCGGAGGCA TGGGCGGTGG TTTAGGAGGT 
GGCTTCAGTG CGGGCGGCGG CTCAGGCAGT GGCTTTGGCC GGGGAGGCGG CGGTGGAATC 
GGCGGTGGAT TTGGCGGCGG CAGCAGCGGT TTCAGCGGTG GCAGCGGCTT TGGCTCCATC 
TCTGGCGCCC GCTATGGAGT CAGTGGCGGG GGCTTCAGCT CGGCCAGCAA CCGGGGCGGC 
AGCATCAAGT TCTCCCAGTC CTCCCAGTCC TCCCAGCGCT ACTCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024304743.1
CDS	66..2216
Translation	MQNALSALATLLCLPPLTAAEFIQTPVPPALCPWGLMVCRQKGTSFAQDQSLPMGVSCRSKVFISHPSSSDSQCPCLTYTLLFAKLLTMSRQASKTSGGGSQGFSGRSAVVSGSSRMSCVAHSGGAGGGAYGFRSGAGGFGSRSLYNLGGNKSISISVAAGGSRAGGFGGGRSSCAFAGGYGGGFGSGYGGGFGGGFGGGRGMGGGFGGAGGFGGAGGFGGAGGFGGPGGFGGSGGFGGPGSLGSPGGFGPGGFPGGIQEVTINQSLLQPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWNLLQQQGTSSISGTNNLEPLFENHINYLRSYLDNILGERGRLDSELKNMEDLVEDFKKKYEDEINKRTAAENEFVTLKKDVDSAYMNKVELQAKVDALIDEIDFLRTLYDAELSQMQSHISDTSVVLSMDNNRSLDLDSIIAEVRAQYEDIAQRSKAEAEALYQTKLGELQTTAGRHGDDLRNTKSEIIELNRMIQRLRAEIEGVKKQNANLQTAIAEAEQHGEMALKDANAKLQELQAALQQAKDDLARLLRDYQELMNVKLALDVEIATYRKLLEGEEYRMSGECPSAVSISVVSSSTTSASAGGYGGGYGGGMGGGLGGGFSAGGGSGSGFGRGGGGGIGGGFGGGSSGFSGGSGFGSISGARYGVSGGGFSSASNRGGSIKFSQSSQSSQRYSR

Target ORF information:

RefSeq Version	XM_024448975.1
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 3 (KRT3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024448975.1

ORF Insert Sequence:

ATGCAGAATG CACTCTCTGC CTTGGCCACC CTACTGTGCC TCCCACCCTT GACTGCCGCA 
GAGTTCATTC AGACTCCAGT TCCTCCAGCC CTGTGTCCAT GGGGCCTCAT GGTGTGCAGG 
CAGAAAGGCA CGTCGTTTGC CCAAGACCAG TCCTTGCCCA TGGGAGTCTC GTGCAGATCC 
AAGGTCTTCA TTTCCCATCC CTCTTCCTCT GACTCTCAGT GTCCCTGCTT AACTTACACT 
CTTCTCTTCG CCAAGCTCCT TACCATGAGC AGACAAGCCA GCAAGACATC TGGTGGCGGG 
AGCCAGGGTT TCTCCGGCCG CTCTGCTGTG GTCTCCGGCA GCAGCAGGAT GAGCTGTGTG 
GCCCACTCTG GGGGAGCTGG CGGAGGGGCC TATGGCTTCC GGAGCGGAGC AGGTGGCTTT 
GGCAGTCGCA GCCTCTACAA CCTGGGCGGC AACAAGAGCA TCTCCATCAG CGTGGCAGCT 
GGCGGCTCCC GGGCTGGAGG CTTTGGGGGA GGGCGGAGCA GCTGTGCCTT TGCAGGTGGC 
TATGGAGGTG GCTTTGGGAG CGGCTATGGA GGTGGCTTTG GTGGTGGCTT TGGTGGTGGC 
AGAGGAATGG GAGGTGGCTT TGGTGGAGCT GGTGGCTTTG GAGGGGCTGG TGGCTTTGGA 
GGGGCTGGTG GCTTTGGTGG TCCTGGTGGC TTTGGTGGGT CTGGTGGCTT TGGTGGGCCT 
GGCAGCTTGG GCAGTCCTGG TGGCTTTGGC CCTGGGGGCT TTCCTGGGGG AATTCAGGAA 
GTGACTATCA ACCAGAGTCT CCTGCAGCCC CTCAATGTGG AGATCGACCC CCAGATTGGG 
CAAGTAAAGG CCCAGGAGCG GGAACAGATC AAGACCCTCA ACAACAAGTT TGCCTCCTTC 
ATTGACAAGG TGCGGTTCCT GGAGCAACAG AACAAAGTCC TGGAGACCAA GTGGAACCTG 
CTCCAGCAGC AGGGCACAAG TTCCATCTCA GGCACAAACA ACCTTGAGCC TCTTTTTGAG 
AATCACATCA ACTACCTGCG GAGCTACCTG GACAACATCC TCGGGGAGAG AGGGCGCCTG 
GACTCTGAGC TGAAGAACAT GGAGGACCTG GTGGAAGACT TCAAGAAGAA ATATGAGGAT 
GAAATCAATA AACGTACAGC TGCTGAGAAT GAATTTGTGA CTCTGAAGAA GGATGTGGAC 
AGTGCCTATA TGAACAAGGT GGAGCTTCAG GCCAAAGTGG ATGCCTTGAT AGATGAGATC 
GACTTCTTAA GGACCCTCTA CGACGCTGAG CTATCTCAGA TGCAGAGCCA CATCAGTGAC 
ACATCTGTGG TGCTGTCCAT GGACAATAAT CGCTCCCTGG ACCTGGACAG CATCATTGCT 
GAAGTTCGTG CACAGTATGA GGATATCGCT CAGAGAAGCA AGGCCGAAGC TGAGGCCCTG 
TACCAGACCA AGTTGGGGGA GCTGCAGACC ACGGCTGGCA GGCATGGGGA TGACCTAAGA 
AATACCAAGA GCGAGATCAT AGAGCTCAAC AGAATGATCC AGAGGCTGCG GGCAGAGATC 
GAGGGTGTCA AGAAGCAGAA TGCCAACCTG CAGACGGCCA TTGCCGAGGC CGAGCAGCAT 
GGAGAGATGG CCCTCAAGGA TGCCAATGCC AAGCTCCAAG AGCTGCAGGC TGCTCTACAG 
CAGGCGAAGG ATGACCTGGC GCGGCTGCTA CGTGACTACC AGGAGCTGAT GAATGTCAAG 
CTGGCCCTGG ACGTGGAGAT CGCCACCTAC CGCAAGCTGC TGGAGGGCGA GGAGTACAGG 
ATGTCTGGAG AGTGTCCGAG TGCTGTCAGC ATCTCCGTGG TCAGCAGCAG CACGACTTCC 
GCCTCCGCAG GTGGCTATGG AGGAGGTTAC GGCGGAGGCA TGGGCGGTGG TTTAGGAGGT 
GGCTTCAGTG CGGGCGGCGG CTCAGGCAGT GGCTTTGGCC GGGGAGGCGG CGGTGGAATC 
GGCGGTGGAT TTGGCGGCGG CAGCAGCGGT TTCAGCGGTG GCAGCGGCTT TGGCTCCATC 
TCTGGCGCCC GCTATGGAGT CAGTGGCGGG GGCTTCAGCT CGGCCAGCAA CCGGGGCGGC 
AGCATCAAGT TCTCCCAGTC CTCCCAGTCC TCCCAGCGCT ACTCCAGATA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KRT3 ( NM_057088.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_476429.2 Homo sapiens keratin 3 (KRT3), mRNA.

Price & Availability↑

CloneID	OHu17842
Clone ID Related Accession (Same CDS sequence)	NM_057088.2 , NM_057088.3
Accession Version	NM_057088.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	keratin, type II cytoskeletal 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from CV571376.1, AJ628418.1, AC107016.20 and BM672628.1. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2006 this sequence version replaced NM_057088.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ628418.1, AK314987.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGAC AAGCCAGCAA GACATCTGGT GGCGGGAGCC AGGGTTTCTC CGGCCGCTCT 
GCTGTGGTCT CCGGCAGCAG CAGGATGAGC TGTGTGGCCC ACTCTGGGGG AGCTGGCGGA 
GGGGCCTATG GCTTCCGGAG CGGAGCAGGT GGCTTTGGCA GTCGCAGCCT CTACAACCTG 
GGCGGCAACA AGAGCATCTC CATCAGCGTG GCAGCTGGCG GCTCCCGGGC TGGAGGCTTT 
GGGGGAGGGC GGAGCAGCTG TGCCTTTGCA GGTGGCTATG GAGGTGGCTT TGGGAGCGGC 
TATGGAGGTG GCTTTGGTGG TGGCTTTGGT GGTGGCAGAG GAATGGGAGG TGGCTTTGGT 
GGAGCTGGTG GCTTTGGAGG GGCTGGTGGC TTTGGAGGGG CTGGTGGCTT TGGTGGTCCT 
GGTGGCTTTG GTGGGTCTGG TGGCTTTGGT GGGCCTGGCA GCTTGGGCAG TCCTGGTGGC 
TTTGGCCCTG GGGGCTTTCC TGGGGGAATT CAGGAAGTGA CTATCAACCA GAGTCTCCTG 
CAGCCCCTCA ATGTGGAGAT CGACCCCCAG ATTGGGCAAG TAAAGGCCCA GGAGCGGGAA 
CAGATCAAGA CCCTCAACAA CAAGTTTGCC TCCTTCATTG ACAAGGTGCG GTTCCTGGAG 
CAACAGAACA AAGTCCTGGA GACCAAGTGG AACCTGCTCC AGCAGCAGGG CACAAGTTCC 
ATCTCAGGCA CAAACAACCT TGAGCCTCTT TTTGAGAATC ACATCAACTA CCTGCGGAGC 
TACCTGGACA ACATCCTCGG GGAGAGAGGG CGCCTGGACT CTGAGCTGAA GAACATGGAG 
GACCTGGTGG AAGACTTCAA GAAGAAATAT GAGGATGAAA TCAATAAACG TACAGCTGCT 
GAGAATGAAT TTGTGACTCT GAAGAAGGAT GTGGACAGTG CCTATATGAA CAAGGTGGAG 
CTTCAGGCCA AAGTGGATGC CTTGATAGAT GAGATCGACT TCTTAAGGAC CCTCTACGAC 
GCTGAGCTAT CTCAGATGCA GAGCCACATC AGTGACACAT CTGTGGTGCT GTCCATGGAC 
AATAATCGCT CCCTGGACCT GGACAGCATC ATTGCTGAAG TTCGTGCACA GTATGAGGAT 
ATCGCTCAGA GAAGCAAGGC CGAAGCTGAG GCCCTGTACC AGACCAAGTT GGGGGAGCTG 
CAGACCACGG CTGGCAGGCA TGGGGATGAC CTAAGAAATA CCAAGAGCGA GATCATAGAG 
CTCAACAGAA TGATCCAGAG GCTGCGGGCA GAGATCGAGG GTGTCAAGAA GCAGAATGCC 
AACCTGCAGA CGGCCATTGC CGAGGCCGAG CAGCATGGAG AGATGGCCCT CAAGGATGCC 
AATGCCAAGC TCCAAGAGCT GCAGGCTGCT CTACAGCAGG CGAAGGATGA CCTGGCGCGG 
CTGCTACGTG ACTACCAGGA GCTGATGAAT GTCAAGCTGG CCCTGGACGT GGAGATCGCC 
ACCTACCGCA AGCTGCTGGA GGGCGAGGAG TACAGGATGT CTGGAGAGTG TCCGAGTGCT 
GTCAGCATCT CCGTGGTCAG CAGCAGCACG ACTTCCGCCT CCGCAGGTGG CTATGGAGGA 
GGTTACGGCG GAGGCATGGG CGGTGGTTTA GGAGGTGGCT TCAGTGCGGG CGGCGGCTCA 
GGCAGTGGCT TTGGCCGGGG AGGCGGCGGT GGAATCGGCG GTGGATTTGG CGGCGGCAGC 
AGCGGTTTCA GCGGTGGCAG CGGCTTTGGC TCCATCTCTG GCGCCCGCTA TGGAGTCAGT 
GGCGGGGGCT TCAGCTCGGC CAGCAACCGG GGCGGCAGCA TCAAGTTCTC CCAGTCCTCC 
CAGTCCTCCC AGCGCTACTC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476429.2
CDS	67..1953
Translation	MSRQASKTSGGGSQGFSGRSAVVSGSSRMSCVAHSGGAGGGAYGFRSGAGGFGSRSLYNLGGNKSISISVAAGGSRAGGFGGGRSSCAFAGGYGGGFGSGYGGGFGGGFGGGRGMGGGFGGAGGFGGAGGFGGAGGFGGPGGFGGSGGFGGPGSLGSPGGFGPGGFPGGIQEVTINQSLLQPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWNLLQQQGTSSISGTNNLEPLFENHINYLRSYLDNILGERGRLDSELKNMEDLVEDFKKKYEDEINKRTAAENEFVTLKKDVDSAYMNKVELQAKVDALIDEIDFLRTLYDAELSQMQSHISDTSVVLSMDNNRSLDLDSIIAEVRAQYEDIAQRSKAEAEALYQTKLGELQTTAGRHGDDLRNTKSEIIELNRMIQRLRAEIEGVKKQNANLQTAIAEAEQHGEMALKDANAKLQELQAALQQAKDDLARLLRDYQELMNVKLALDVEIATYRKLLEGEEYRMSGECPSAVSISVVSSSTTSASAGGYGGGYGGGMGGGLGGGFSAGGGSGSGFGRGGGGGIGGGFGGGSSGFSGGSGFGSISGARYGVSGGGFSSASNRGGSIKFSQSSQSSQRYSR

Target ORF information:

RefSeq Version	NM_057088.2
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 3 (KRT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057088.2

ORF Insert Sequence:

ATGAGCAGAC AAGCCAGCAA GACATCTGGT GGCGGGAGCC AGGGTTTCTC CGGCCGCTCT 
GCTGTGGTCT CCGGCAGCAG CAGGATGAGC TGTGTGGCCC ACTCTGGGGG AGCTGGCGGA 
GGGGCCTATG GCTTCCGGAG CGGAGCAGGT GGCTTTGGCA GTCGCAGCCT CTACAACCTG 
GGCGGCAACA AGAGCATCTC CATCAGCGTG GCAGCTGGCG GCTCCCGGGC TGGAGGCTTT 
GGGGGAGGGC GGAGCAGCTG TGCCTTTGCA GGTGGCTATG GAGGTGGCTT TGGGAGCGGC 
TATGGAGGTG GCTTTGGTGG TGGCTTTGGT GGTGGCAGAG GAATGGGAGG TGGCTTTGGT 
GGAGCTGGTG GCTTTGGAGG GGCTGGTGGC TTTGGAGGGG CTGGTGGCTT TGGTGGTCCT 
GGTGGCTTTG GTGGGTCTGG TGGCTTTGGT GGGCCTGGCA GCTTGGGCAG TCCTGGTGGC 
TTTGGCCCTG GGGGCTTTCC TGGGGGAATT CAGGAAGTGA CTATCAACCA GAGTCTCCTG 
CAGCCCCTCA ATGTGGAGAT CGACCCCCAG ATTGGGCAAG TAAAGGCCCA GGAGCGGGAA 
CAGATCAAGA CCCTCAACAA CAAGTTTGCC TCCTTCATTG ACAAGGTGCG GTTCCTGGAG 
CAACAGAACA AAGTCCTGGA GACCAAGTGG AACCTGCTCC AGCAGCAGGG CACAAGTTCC 
ATCTCAGGCA CAAACAACCT TGAGCCTCTT TTTGAGAATC ACATCAACTA CCTGCGGAGC 
TACCTGGACA ACATCCTCGG GGAGAGAGGG CGCCTGGACT CTGAGCTGAA GAACATGGAG 
GACCTGGTGG AAGACTTCAA GAAGAAATAT GAGGATGAAA TCAATAAACG TACAGCTGCT 
GAGAATGAAT TTGTGACTCT GAAGAAGGAT GTGGACAGTG CCTATATGAA CAAGGTGGAG 
CTTCAGGCCA AAGTGGATGC CTTGATAGAT GAGATCGACT TCTTAAGGAC CCTCTACGAC 
GCTGAGCTAT CTCAGATGCA GAGCCACATC AGTGACACAT CTGTGGTGCT GTCCATGGAC 
AATAATCGCT CCCTGGACCT GGACAGCATC ATTGCTGAAG TTCGTGCACA GTATGAGGAT 
ATCGCTCAGA GAAGCAAGGC CGAAGCTGAG GCCCTGTACC AGACCAAGTT GGGGGAGCTG 
CAGACCACGG CTGGCAGGCA TGGGGATGAC CTAAGAAATA CCAAGAGCGA GATCATAGAG 
CTCAACAGAA TGATCCAGAG GCTGCGGGCA GAGATCGAGG GTGTCAAGAA GCAGAATGCC 
AACCTGCAGA CGGCCATTGC CGAGGCCGAG CAGCATGGAG AGATGGCCCT CAAGGATGCC 
AATGCCAAGC TCCAAGAGCT GCAGGCTGCT CTACAGCAGG CGAAGGATGA CCTGGCGCGG 
CTGCTACGTG ACTACCAGGA GCTGATGAAT GTCAAGCTGG CCCTGGACGT GGAGATCGCC 
ACCTACCGCA AGCTGCTGGA GGGCGAGGAG TACAGGATGT CTGGAGAGTG TCCGAGTGCT 
GTCAGCATCT CCGTGGTCAG CAGCAGCACG ACTTCCGCCT CCGCAGGTGG CTATGGAGGA 
GGTTACGGCG GAGGCATGGG CGGTGGTTTA GGAGGTGGCT TCAGTGCGGG CGGCGGCTCA 
GGCAGTGGCT TTGGCCGGGG AGGCGGCGGT GGAATCGGCG GTGGATTTGG CGGCGGCAGC 
AGCGGTTTCA GCGGTGGCAG CGGCTTTGGC TCCATCTCTG GCGCCCGCTA TGGAGTCAGT 
GGCGGGGGCT TCAGCTCGGC CAGCAACCGG GGCGGCAGCA TCAAGTTCTC CCAGTCCTCC 
CAGTCCTCCC AGCGCTACTC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KRT3 ( NM_057088.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_476429.2 Homo sapiens keratin 3 (KRT3), mRNA.

Price & Availability↑

CloneID	OHu17842
Clone ID Related Accession (Same CDS sequence)	NM_057088.2 , NM_057088.3
Accession Version	NM_057088.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1887bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	keratin, type II cytoskeletal 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Michael Rogers. The reference sequence was derived from AC107016.20, CV571376.1, AJ628418.1 and BM672628.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_057088.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK314987.1, AJ628418.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156670 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000417996.2/ ENSP00000413479.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGCAGAC AAGCCAGCAA GACATCTGGT GGCGGGAGCC AGGGTTTCTC CGGCCGCTCT 
GCTGTGGTCT CCGGCAGCAG CAGGATGAGC TGTGTGGCCC ACTCTGGGGG AGCTGGCGGA 
GGGGCCTATG GCTTCCGGAG CGGAGCAGGT GGCTTTGGCA GTCGCAGCCT CTACAACCTG 
GGCGGCAACA AGAGCATCTC CATCAGCGTG GCAGCTGGCG GCTCCCGGGC TGGAGGCTTT 
GGGGGAGGGC GGAGCAGCTG TGCCTTTGCA GGTGGCTATG GAGGTGGCTT TGGGAGCGGC 
TATGGAGGTG GCTTTGGTGG TGGCTTTGGT GGTGGCAGAG GAATGGGAGG TGGCTTTGGT 
GGAGCTGGTG GCTTTGGAGG GGCTGGTGGC TTTGGAGGGG CTGGTGGCTT TGGTGGTCCT 
GGTGGCTTTG GTGGGTCTGG TGGCTTTGGT GGGCCTGGCA GCTTGGGCAG TCCTGGTGGC 
TTTGGCCCTG GGGGCTTTCC TGGGGGAATT CAGGAAGTGA CTATCAACCA GAGTCTCCTG 
CAGCCCCTCA ATGTGGAGAT CGACCCCCAG ATTGGGCAAG TAAAGGCCCA GGAGCGGGAA 
CAGATCAAGA CCCTCAACAA CAAGTTTGCC TCCTTCATTG ACAAGGTGCG GTTCCTGGAG 
CAACAGAACA AAGTCCTGGA GACCAAGTGG AACCTGCTCC AGCAGCAGGG CACAAGTTCC 
ATCTCAGGCA CAAACAACCT TGAGCCTCTT TTTGAGAATC ACATCAACTA CCTGCGGAGC 
TACCTGGACA ACATCCTCGG GGAGAGAGGG CGCCTGGACT CTGAGCTGAA GAACATGGAG 
GACCTGGTGG AAGACTTCAA GAAGAAATAT GAGGATGAAA TCAATAAACG TACAGCTGCT 
GAGAATGAAT TTGTGACTCT GAAGAAGGAT GTGGACAGTG CCTATATGAA CAAGGTGGAG 
CTTCAGGCCA AAGTGGATGC CTTGATAGAT GAGATCGACT TCTTAAGGAC CCTCTACGAC 
GCTGAGCTAT CTCAGATGCA GAGCCACATC AGTGACACAT CTGTGGTGCT GTCCATGGAC 
AATAATCGCT CCCTGGACCT GGACAGCATC ATTGCTGAAG TTCGTGCACA GTATGAGGAT 
ATCGCTCAGA GAAGCAAGGC CGAAGCTGAG GCCCTGTACC AGACCAAGTT GGGGGAGCTG 
CAGACCACGG CTGGCAGGCA TGGGGATGAC CTAAGAAATA CCAAGAGCGA GATCATAGAG 
CTCAACAGAA TGATCCAGAG GCTGCGGGCA GAGATCGAGG GTGTCAAGAA GCAGAATGCC 
AACCTGCAGA CGGCCATTGC CGAGGCCGAG CAGCATGGAG AGATGGCCCT CAAGGATGCC 
AATGCCAAGC TCCAAGAGCT GCAGGCTGCT CTACAGCAGG CGAAGGATGA CCTGGCGCGG 
CTGCTACGTG ACTACCAGGA GCTGATGAAT GTCAAGCTGG CCCTGGACGT GGAGATCGCC 
ACCTACCGCA AGCTGCTGGA GGGCGAGGAG TACAGGATGT CTGGAGAGTG TCCGAGTGCT 
GTCAGCATCT CCGTGGTCAG CAGCAGCACG ACTTCCGCCT CCGCAGGTGG CTATGGAGGA 
GGTTACGGCG GAGGCATGGG CGGTGGTTTA GGAGGTGGCT TCAGTGCGGG CGGCGGCTCA 
GGCAGTGGCT TTGGCCGGGG AGGCGGCGGT GGAATCGGCG GTGGATTTGG CGGCGGCAGC 
AGCGGTTTCA GCGGTGGCAG CGGCTTTGGC TCCATCTCTG GCGCCCGCTA TGGAGTCAGT 
GGCGGGGGCT TCAGCTCGGC CAGCAACCGG GGCGGCAGCA TCAAGTTCTC CCAGTCCTCC 
CAGTCCTCCC AGCGCTACTC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_476429.2
CDS	76..1962
Translation	MSRQASKTSGGGSQGFSGRSAVVSGSSRMSCVAHSGGAGGGAYGFRSGAGGFGSRSLYNLGGNKSISISVAAGGSRAGGFGGGRSSCAFAGGYGGGFGSGYGGGFGGGFGGGRGMGGGFGGAGGFGGAGGFGGAGGFGGPGGFGGSGGFGGPGSLGSPGGFGPGGFPGGIQEVTINQSLLQPLNVEIDPQIGQVKAQEREQIKTLNNKFASFIDKVRFLEQQNKVLETKWNLLQQQGTSSISGTNNLEPLFENHINYLRSYLDNILGERGRLDSELKNMEDLVEDFKKKYEDEINKRTAAENEFVTLKKDVDSAYMNKVELQAKVDALIDEIDFLRTLYDAELSQMQSHISDTSVVLSMDNNRSLDLDSIIAEVRAQYEDIAQRSKAEAEALYQTKLGELQTTAGRHGDDLRNTKSEIIELNRMIQRLRAEIEGVKKQNANLQTAIAEAEQHGEMALKDANAKLQELQAALQQAKDDLARLLRDYQELMNVKLALDVEIATYRKLLEGEEYRMSGECPSAVSISVVSSSTTSASAGGYGGGYGGGMGGGLGGGFSAGGGSGSGFGRGGGGGIGGGFGGGSSGFSGGSGFGSISGARYGVSGGGFSSASNRGGSIKFSQSSQSSQRYSR

Target ORF information:

RefSeq Version	NM_057088.3
Organism	Homo sapiens(human)
Definition	Homo sapiens keratin 3 (KRT3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_057088.3

ORF Insert Sequence:

ATGAGCAGAC AAGCCAGCAA GACATCTGGT GGCGGGAGCC AGGGTTTCTC CGGCCGCTCT 
GCTGTGGTCT CCGGCAGCAG CAGGATGAGC TGTGTGGCCC ACTCTGGGGG AGCTGGCGGA 
GGGGCCTATG GCTTCCGGAG CGGAGCAGGT GGCTTTGGCA GTCGCAGCCT CTACAACCTG 
GGCGGCAACA AGAGCATCTC CATCAGCGTG GCAGCTGGCG GCTCCCGGGC TGGAGGCTTT 
GGGGGAGGGC GGAGCAGCTG TGCCTTTGCA GGTGGCTATG GAGGTGGCTT TGGGAGCGGC 
TATGGAGGTG GCTTTGGTGG TGGCTTTGGT GGTGGCAGAG GAATGGGAGG TGGCTTTGGT 
GGAGCTGGTG GCTTTGGAGG GGCTGGTGGC TTTGGAGGGG CTGGTGGCTT TGGTGGTCCT 
GGTGGCTTTG GTGGGTCTGG TGGCTTTGGT GGGCCTGGCA GCTTGGGCAG TCCTGGTGGC 
TTTGGCCCTG GGGGCTTTCC TGGGGGAATT CAGGAAGTGA CTATCAACCA GAGTCTCCTG 
CAGCCCCTCA ATGTGGAGAT CGACCCCCAG ATTGGGCAAG TAAAGGCCCA GGAGCGGGAA 
CAGATCAAGA CCCTCAACAA CAAGTTTGCC TCCTTCATTG ACAAGGTGCG GTTCCTGGAG 
CAACAGAACA AAGTCCTGGA GACCAAGTGG AACCTGCTCC AGCAGCAGGG CACAAGTTCC 
ATCTCAGGCA CAAACAACCT TGAGCCTCTT TTTGAGAATC ACATCAACTA CCTGCGGAGC 
TACCTGGACA ACATCCTCGG GGAGAGAGGG CGCCTGGACT CTGAGCTGAA GAACATGGAG 
GACCTGGTGG AAGACTTCAA GAAGAAATAT GAGGATGAAA TCAATAAACG TACAGCTGCT 
GAGAATGAAT TTGTGACTCT GAAGAAGGAT GTGGACAGTG CCTATATGAA CAAGGTGGAG 
CTTCAGGCCA AAGTGGATGC CTTGATAGAT GAGATCGACT TCTTAAGGAC CCTCTACGAC 
GCTGAGCTAT CTCAGATGCA GAGCCACATC AGTGACACAT CTGTGGTGCT GTCCATGGAC 
AATAATCGCT CCCTGGACCT GGACAGCATC ATTGCTGAAG TTCGTGCACA GTATGAGGAT 
ATCGCTCAGA GAAGCAAGGC CGAAGCTGAG GCCCTGTACC AGACCAAGTT GGGGGAGCTG 
CAGACCACGG CTGGCAGGCA TGGGGATGAC CTAAGAAATA CCAAGAGCGA GATCATAGAG 
CTCAACAGAA TGATCCAGAG GCTGCGGGCA GAGATCGAGG GTGTCAAGAA GCAGAATGCC 
AACCTGCAGA CGGCCATTGC CGAGGCCGAG CAGCATGGAG AGATGGCCCT CAAGGATGCC 
AATGCCAAGC TCCAAGAGCT GCAGGCTGCT CTACAGCAGG CGAAGGATGA CCTGGCGCGG 
CTGCTACGTG ACTACCAGGA GCTGATGAAT GTCAAGCTGG CCCTGGACGT GGAGATCGCC 
ACCTACCGCA AGCTGCTGGA GGGCGAGGAG TACAGGATGT CTGGAGAGTG TCCGAGTGCT 
GTCAGCATCT CCGTGGTCAG CAGCAGCACG ACTTCCGCCT CCGCAGGTGG CTATGGAGGA 
GGTTACGGCG GAGGCATGGG CGGTGGTTTA GGAGGTGGCT TCAGTGCGGG CGGCGGCTCA 
GGCAGTGGCT TTGGCCGGGG AGGCGGCGGT GGAATCGGCG GTGGATTTGG CGGCGGCAGC 
AGCGGTTTCA GCGGTGGCAG CGGCTTTGGC TCCATCTCTG GCGCCCGCTA TGGAGTCAGT 
GGCGGGGGCT TCAGCTCGGC CAGCAACCGG GGCGGCAGCA TCAAGTTCTC CCAGTCCTCC 
CAGTCCTCCC AGCGCTACTC CAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Human aniridia limbal epithelial cells lack expression of keratins K3 and K12.
Experimental eye research167100-109(2017 Nov)
Latta L,Viestenz A,Stachon T,Colanesi S,Szentm?ry N,Seitz B,K?smann-Kellner B

PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.
Scientific reports620807(2016 Feb)
Sasamoto Y,Hayashi R,Park SJ,Saito-Adachi M,Suzuki Y,Kawasaki S,Quantock AJ,Nakai K,Tsujikawa M,Nishida K

Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family.
The Journal of international medical research41(2)511-8(2013 Apr)
Cao W,Yan M,Hao Q,Wang S,Wu L,Liu Q,Li M,Biddle FG,Wu W

Genetics of Meesmann corneal dystrophy: a novel mutation in the keratin 3 gene in an asymptomatic family suggests genotype-phenotype correlation.
Molecular vision141713-8(2008 Sep)
Szaflik JP,O?dak M,Maksym RB,Kami?ska A,Pollak A,Udziela M,P?oski R,Szaflik J

New consensus nomenclature for mammalian keratins.
The Journal of cell biology174(2)169-74(2006 Jul)
Schweizer J,Bowden PE,Coulombe PA,Langbein L,Lane EB,Magin TM,Maltais L,Omary MB,Parry DA,Rogers MA,Wright MW

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KRT3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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