ARF4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ARF4
Entrez Gene ID	378
Full Name	ADP ribosylation factor 4
Synonyms	ARF2
General protein information	Preferred Names ADP ribosylation factor 4 Names ADP-ribosylation factor 4 ADP-ribosylation factor 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p14.3
Summary	This gene is a member of the human ARF gene family whose members encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The gene products include 5 ARF proteins and 11 ARF-like proteins and constitute one family of the RAS superfamily. The ARF proteins are categorized as class I, class II and class III; this gene is a class II member. The members of each class share a common gene organization. The ARF4 gene spans approximately 12kb and contains six exons and five introns. This gene is the most divergent member of the human ARFs. Conflicting map positions at 3p14 or 3p21 have been reported for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	601177

mRNA and Protein(s)

mRNA	Protein	Name
NM_001660.4	NP_001651.1	ADP-ribosylation factor 4
NM_001660.3	NP_001651.1	ADP-ribosylation factor 4

Pathways from BioSystems

Homologies

	ARF5	XP_001103974.1
Homo sapiens (human)	ARF4	NP_001651.1
Pan troglodytes (chimpanzee)	ARF4	XP_003950181.1
Gallus gallus (chicken)	ARF4	XP_001232784.3
Canis lupus familiaris (dog)	ARF4	XP_849047.1
Drosophila melanogaster (fruit fly)	Arf102F	NP_524631.1
Xenopus tropicalis (tropical clawed frog)	arf4	NP_001025648.1
Mus musculus (house mouse)	Arf4	NP_031505.1
Danio rerio (zebrafish)	arf4a	NP_001003590.1
Rattus norvegicus (Norway rat)	Arf4	NP_077065.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002931	XP_003436315.1

Gene Ontology
Bibliography

Related articles in PubMed

Interplay between hepatitis C virus and ARF4.
Zhang N, Ke Y, Zhang L
Virologica Sinica32(6)533-536(2017 Dec)

Commensal microbiota-induced microRNA modulates intestinal epithelial permeability through the small GTPase ARF4.
Nakata K, Sugi Y, Narabayashi H, Kobayakawa T, Nakanishi Y, Tsuda M, Hosono A, Kaminogawa S, Hanazawa S, Takahashi K
The Journal of biological chemistry292(37)15426-15433(2017 09)

MiR-221-3p targets ARF4 and inhibits the proliferation and migration of epithelial ovarian cancer cells.
Wu Q, Ren X, Zhang Y, Fu X, Li Y, Peng Y, Xiao Q, Li T, Ouyang C, Hu Y, Zhang Y, Zhou W, Yan W, Guo K, Li W, Hu Y, Yang X, Shu G, Xue H, Wei Z, Luo Y, Yin G
Biochemical and biophysical research communications(2017 Jan)

Structure of Rab11-FIP3-Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11.
Vetter M, Stehle R, Basquin C, Lorentzen E
Nature structural & molecular biology22(9)695-702(2015 Sep)

WLS retrograde transport to the endoplasmic reticulum during Wnt secretion.
Yu J, Chia J, Canning CA, Jones CM, Bard FA, Virshup DM
Developmental cell29(3)277-91(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that, when compared to intestinal epithelial cells (IECs) from germ-free mice, IECs from mice with commensal bacteria exhibit increased expression of microRNA-21-5p; up-regulation of expression of microRNA-21-5p appears to increase IEC permeability and up-regulates ADP ribosylation factor 4 (Arf4/ARF4) in mouse and human cells.
Title: Commensal microbiota-induced microRNA modulates intestinal epithelial permeability through the small GTPase ARF4.

A CREB3-ARF4 signalling cascade may be part of a Golgi stress response set in motion by stimuli compromising Golgi capacity.
Title: A CREB3-ARF4 signalling pathway mediates the response to Golgi stress and susceptibility to pathogens.

ARF1+ARF4 are required for integrity of recycling endosomes but are involved in distinct transport pathways: the pair regulates retrograde transport from endosomes to the TGN.
Title: ARF1 and ARF4 regulate recycling endosomal morphology and retrograde transport from endosomes to the Golgi apparatus.

a crucial role for class II Arf proteins (Arf4 and Arf5) in the dengue flavivirus life cycle
Title: Class II ADP-ribosylation factors are required for efficient secretion of dengue viruses.

The lipid droplets deposition and the cellular triacylglycerol content are significantly increased by siRNA-mediated depletion of Arf4.
Title: GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis.

The following ARF4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ARF4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14888	NM_001660.4 Latest version!	Homo sapiens ADP ribosylation factor 4 (ARF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu14888	NM_001660.3	Homo sapiens ADP ribosylation factor 4 (ARF4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ARF4 ( NM_001660.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001651.1 Homo sapiens ADP ribosylation factor 4 (ARF4), mRNA.

Price & Availability↑

CloneID	OHu14888
Clone ID Related Accession (Same CDS sequence)	NM_001660.4 , NM_001660.3
Accession Version	NM_001660.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA259855.1 and BC003364.1. On Nov 22, 2018 this sequence version replaced NM_001660.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1001558.1, SRR3476690.1024844.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000303436.11/ ENSP00000306010.6 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCTCA CTATCTCCTC CCTCTTCTCC CGACTATTTG GCAAGAAGCA GATGCGCATT 
TTGATGGTTG GATTGGATGC TGCTGGCAAG ACAACCATTC TGTATAAACT GAAGTTAGGG 
GAGATAGTCA CCACCATTCC TACCATTGGT TTTAATGTGG AAACAGTAGA ATATAAGAAC 
ATTTGTTTCA CAGTATGGGA TGTTGGTGGT CAAGATAGAA TTAGGCCTCT CTGGAAGCAT 
TACTTCCAGA ATACCCAGGG TCTTATTTTT GTGGTAGATA GCAACGATCG TGAAAGAATT 
CAGGAAGTAG CAGATGAGCT GCAGAAAATG CTTCTGGTAG ATGAATTGAG AGATGCAGTG 
CTGCTACTTT TTGCAAACAA ACAGGATTTG CCAAATGCTA TGGCCATCAG TGAAATGACA 
GATAAACTAG GGCTTCAGTC TCTTCGTAAC AGAACATGGT ATGTTCAAGC CACTTGTGCA 
ACACAAGGAA CTGGTCTGTA TGAAGGACTT GACTGGCTGT CAAATGAGCT TTCAAAACGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001651.1
CDS	205..747
Translation	MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR

Target ORF information:

RefSeq Version	NM_001660.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 4 (ARF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001660.4

ORF Insert Sequence:

ATGGGCCTCA CTATCTCCTC CCTCTTCTCC CGACTATTTG GCAAGAAGCA GATGCGCATT 
TTGATGGTTG GATTGGATGC TGCTGGCAAG ACAACCATTC TGTATAAACT GAAGTTAGGG 
GAGATAGTCA CCACCATTCC TACCATTGGT TTTAATGTGG AAACAGTAGA ATATAAGAAC 
ATTTGTTTCA CAGTATGGGA TGTTGGTGGT CAAGATAGAA TTAGGCCTCT CTGGAAGCAT 
TACTTCCAGA ATACCCAGGG TCTTATTTTT GTGGTAGATA GCAACGATCG TGAAAGAATT 
CAGGAAGTAG CAGATGAGCT GCAGAAAATG CTTCTGGTAG ATGAATTGAG AGATGCAGTG 
CTGCTACTTT TTGCAAACAA ACAGGATTTG CCAAATGCTA TGGCCATCAG TGAAATGACA 
GATAAACTAG GGCTTCAGTC TCTTCGTAAC AGAACATGGT ATGTTCAAGC CACTTGTGCA 
ACACAAGGAA CTGGTCTGTA TGAAGGACTT GACTGGCTGT CAAATGAGCT TTCAAAACGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ARF4 ( NM_001660.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001651.1 Homo sapiens ADP ribosylation factor 4 (ARF4), mRNA.

Price & Availability↑

CloneID	OHu14888
Clone ID Related Accession (Same CDS sequence)	NM_001660.4 , NM_001660.3
Accession Version	NM_001660.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 543bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	ADP-ribosylation factor 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA259855.1 and BC003364.1. On May 7, 2008 this sequence version replaced NM_001660.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.206172.1, SRR1163658.251566.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2149398, SAMEA2154405 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGGCCTCA CTATCTCCTC CCTCTTCTCC CGACTATTTG GCAAGAAGCA GATGCGCATT 
TTGATGGTTG GATTGGATGC TGCTGGCAAG ACAACCATTC TGTATAAACT GAAGTTAGGG 
GAGATAGTCA CCACCATTCC TACCATTGGT TTTAATGTGG AAACAGTAGA ATATAAGAAC 
ATTTGTTTCA CAGTATGGGA TGTTGGTGGT CAAGATAGAA TTAGGCCTCT CTGGAAGCAT 
TACTTCCAGA ATACCCAGGG TCTTATTTTT GTGGTAGATA GCAACGATCG TGAAAGAATT 
CAGGAAGTAG CAGATGAGCT GCAGAAAATG CTTCTGGTAG ATGAATTGAG AGATGCAGTG 
CTGCTACTTT TTGCAAACAA ACAGGATTTG CCAAATGCTA TGGCCATCAG TGAAATGACA 
GATAAACTAG GGCTTCAGTC TCTTCGTAAC AGAACATGGT ATGTTCAAGC CACTTGTGCA 
ACACAAGGAA CTGGTCTGTA TGAAGGACTT GACTGGCTGT CAAATGAGCT TTCAAAACGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001651.1
CDS	349..891
Translation	MGLTISSLFSRLFGKKQMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYKNICFTVWDVGGQDRIRPLWKHYFQNTQGLIFVVDSNDRERIQEVADELQKMLLVDELRDAVLLLFANKQDLPNAMAISEMTDKLGLQSLRNRTWYVQATCATQGTGLYEGLDWLSNELSKR

Target ORF information:

RefSeq Version	NM_001660.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ADP ribosylation factor 4 (ARF4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001660.3

ORF Insert Sequence:

ATGGGCCTCA CTATCTCCTC CCTCTTCTCC CGACTATTTG GCAAGAAGCA GATGCGCATT 
TTGATGGTTG GATTGGATGC TGCTGGCAAG ACAACCATTC TGTATAAACT GAAGTTAGGG 
GAGATAGTCA CCACCATTCC TACCATTGGT TTTAATGTGG AAACAGTAGA ATATAAGAAC 
ATTTGTTTCA CAGTATGGGA TGTTGGTGGT CAAGATAGAA TTAGGCCTCT CTGGAAGCAT 
TACTTCCAGA ATACCCAGGG TCTTATTTTT GTGGTAGATA GCAACGATCG TGAAAGAATT 
CAGGAAGTAG CAGATGAGCT GCAGAAAATG CTTCTGGTAG ATGAATTGAG AGATGCAGTG 
CTGCTACTTT TTGCAAACAA ACAGGATTTG CCAAATGCTA TGGCCATCAG TGAAATGACA 
GATAAACTAG GGCTTCAGTC TCTTCGTAAC AGAACATGGT ATGTTCAAGC CACTTGTGCA 
ACACAAGGAA CTGGTCTGTA TGAAGGACTT GACTGGCTGT CAAATGAGCT TTCAAAACGT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Interplay between hepatitis C virus and ARF4.
Virologica Sinica32(6)533-536(2017 Dec)
Zhang N,Ke Y,Zhang L

Commensal microbiota-induced microRNA modulates intestinal epithelial permeability through the small GTPase ARF4.
The Journal of biological chemistry292(37)15426-15433(2017 09)
Nakata K,Sugi Y,Narabayashi H,Kobayakawa T,Nakanishi Y,Tsuda M,Hosono A,Kaminogawa S,Hanazawa S,Takahashi K

MiR-221-3p targets ARF4 and inhibits the proliferation and migration of epithelial ovarian cancer cells.
Biochemical and biophysical research communications(2017 Jan)
Wu Q,Ren X,Zhang Y,Fu X,Li Y,Peng Y,Xiao Q,Li T,Ouyang C,Hu Y,Zhang Y,Zhou W,Yan W,Guo K,Li W,Hu Y,Yang X,Shu G,Xue H,Wei Z,Luo Y,Yin G

Structure of Rab11-FIP3-Rabin8 reveals simultaneous binding of FIP3 and Rabin8 effectors to Rab11.
Nature structural & molecular biology22(9)695-702(2015 Sep)
Vetter M,Stehle R,Basquin C,Lorentzen E

WLS retrograde transport to the endoplasmic reticulum during Wnt secretion.
Developmental cell29(3)277-91(2014 May)
Yu J,Chia J,Canning CA,Jones CM,Bard FA,Virshup DM

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ARF4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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