KCNMB1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KCNMB1
Entrez Gene ID	3779
Full Name	potassium calcium-activated channel subfamily M regulatory beta subunit 1
Synonyms	BKbeta1,K(VCA)beta,SLO-BETA,hbeta1,hslo-beta,k(VCA)beta-1,slo-beta-1
General protein information	Preferred Names potassium calcium-activated channel subfamily M regulatory beta subunit 1 Names calcium-activated potassium channel subunit beta-1 BK channel beta subunit 1 BK channel subunit beta-1 MaxiK channel beta-subunit 1 big potassium channel beta subunit 1 calcium-activated potassium channel, subfamily M subunit beta-1 charybdotoxin receptor subunit beta-1 large conductance Ca2+-activated K+ channel beta 1 subunit maxi K channel subunit beta-1 potassium channel subfamily M regulatory beta subunit 1 potassium large conductance calcium-activated channel, subfamily M, beta member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q35.1
Summary	MaxiK channels are large conductance, voltage and calcium-sensitive potassium channels which are fundamental to the control of smooth muscle tone and neuronal excitability. MaxiK channels can be formed by 2 subunits: the pore-forming alpha subunit and the product of this gene, the modulatory beta subunit. Intracellular calcium regulates the physical association between the alpha and beta subunits. [provided by RefSeq, Jul 2008].
Disorder MIM:	603951
Disorder Html:	{Hypertension, diastolic, resistance to}, 608622 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_004137.3	NP_004128.1	calcium-activated potassium channel subunit beta-1
NM_004137.4	NP_004128.1	calcium-activated potassium channel subunit beta-1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	KCNMB1	NP_001001141.1
Mus musculus (house mouse)	Kcnmb1	NP_112446.2
Pan troglodytes (chimpanzee)	KCNMB1	XP_518094.1
Gallus gallus (chicken)	KCNMB1	NP_989933.1
Canis lupus familiaris (dog)	KCNMB1	NP_001003299.1
Homo sapiens (human)	KCNMB1	NP_004128.1
	KCNMB1	XP_001092823.1
Rattus norvegicus (Norway rat)	Kcnmb1	NP_062146.1

Gene Ontology
Bibliography

Related articles in PubMed

Regulation of vascular large-conductance calcium-activated potassium channels by Nrf2 signalling.
Li Y, Wang XL, Sun X, Chai Q, Li J, Thompson B, Shen WK, Lu T, Lee HC
Diabetes & vascular disease research14(4)353-362(2017 Jul)

The unique N-terminal sequence of the BKCa channel ?-subunit determines its modulation by ?-subunits.
Lorca RA, Ma X, England SK
PloS one12(7)e0182068(2017)

?1-subunit-induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel.
Castillo JP, S?nchez-Rodr?guez JE, Hyde HC, Zaelzer CA, Aguayo D, Sep?lveda RV, Luk LY, Kent SB, Gonzalez-Nilo FD, Bezanilla F, Latorre R
Proceedings of the National Academy of Sciences of the United States of America113(23)E3231-9(2016 Jun)

Molecular mechanism underlying ?1 regulation in voltage- and calcium-activated potassium (BK) channels.
Castillo K, Contreras GF, Pupo A, Torres YP, Neely A, Gonz?lez C, Latorre R
Proceedings of the National Academy of Sciences of the United States of America112(15)4809-14(2015 Apr)

Two distinct effects of PIP2 underlie auxiliary subunit-dependent modulation of Slo1 BK channels.
Tian Y, Ullrich F, Xu R, Heinemann SH, Hou S, Hoshi T
The Journal of general physiology145(4)331-43(2015 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression of vascular large-conductance calcium-activated potassium channel Kcnmb1 is regulated by Nrf2 signaling.
Title: Regulation of vascular large-conductance calcium-activated potassium channels by Nrf2 signalling.

These results provide further insights into the mechanism of modulation of the different N-terminal regions of the BKCa channel by beta-subunits.
Title: The unique N-terminal sequence of the BKCa channel ?-subunit determines its modulation by ?-subunits.

By introducing lanthanide binding tags in the extracellular region of the alpha- or beta1-subunit, we determined (i) a basic extracellular map of the BK channel, (ii) beta1-subunit-induced rearrangements of the voltage sensor in alpha-subunits, and (iii) the relative position of the beta1-subunit within the alpha/beta1-subunit complex.
Title: ?1-subunit-induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel.

Data show that two lysine residues that are unique to the N terminus of calcium-activated potassium channel subunit beta-1 were identified to be sufficient for voltage-sensor modulation.
Title: Molecular mechanism underlying ?1 regulation in voltage- and calcium-activated potassium (BK) channels.

To determine the relationship between atherosclerosis and KCNMB1, we studied some atherogenic factors affecting vascular tone. Blood of atherosclerotic patients shows increased concentration of 7-ketocholesterol, possibly a harmful lipid to blood vessels.
Title: 7-Ketocholesterol induces the reduction of KCNMB1 in atherosclerotic blood vessels.

The following KCNMB1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNMB1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19847	NM_004137.4 Latest version!	Homo sapiens potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu19847	NM_004137.3	Homo sapiens potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCNMB1 ( NM_004137.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004128.1 Homo sapiens potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), mRNA.

Price & Availability↑

CloneID	OHu19847
Clone ID Related Accession (Same CDS sequence)	NM_004137.3 , NM_004137.4
Accession Version	NM_004137.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-17
Organism	Homo sapiens(human)
Product	calcium-activated potassium channel subunit beta-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA619309.1, U25138.1 and AA464625.1. This sequence is a reference standard in the RefSeqGene project. On Feb 13, 2013 this sequence version replaced NM_004137.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.287664.1, U25138.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159368 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGAAGA AGCTGGTGAT GGCCCAGAAG CGGGGAGAGA CACGAGCCCT TTGCCTGGGT 
GTAACCATGG TGGTGTGTGC CGTCATCACC TACTACATCC TGGTCACGAC TGTGCTGCCC 
CTCTACCAGA AAAGCGTGTG GACCCAGGAA TCCAAGTGCC ACCTGATTGA GACCAACATC 
AGGGACCAGG AGGAGCTGAA GGGCAAGAAG GTGCCCCAGT ACCCATGCCT GTGGGTCAAC 
GTGTCAGCTG CCGGCAGGTG GGCTGTGCTG TACCACACGG AGGACACTCG GGACCAGAAC 
CAGCAGTGCT CCTACATCCC AGGCAGCGTG GACAATTACC AGACGGCCCG GGCCGACGTG 
GAGAAGGTCA GAGCCAAATT CCAAGAGCAG CAGGTCTTCT ACTGCTTCTC CGCACCTCGG 
GGGAACGAAA CCAGCGTCCT ATTCCAGCGC CTCTACGGGC CCCAGGCCCT CCTCTTCTCC 
CTCTTCTGGC CCACCTTCCT GCTGACCGGT GGCCTCCTCA TTATCGCCAT GGTGAAGAGC 
AACCAGTACC TGTCCATCCT GGCGGCCCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004128.1
CDS	444..1019
Translation	MVKKLVMAQKRGETRALCLGVTMVVCAVITYYILVTTVLPLYQKSVWTQESKCHLIETNIRDQEELKGKKVPQYPCLWVNVSAAGRWAVLYHTEDTRDQNQQCSYIPGSVDNYQTARADVEKVRAKFQEQQVFYCFSAPRGNETSVLFQRLYGPQALLFSLFWPTFLLTGGLLIIAMVKSNQYLSILAAQK

Target ORF information:

RefSeq Version	NM_004137.3
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004137.3

ORF Insert Sequence:

ATGGTGAAGA AGCTGGTGAT GGCCCAGAAG CGGGGAGAGA CACGAGCCCT TTGCCTGGGT 
GTAACCATGG TGGTGTGTGC CGTCATCACC TACTACATCC TGGTCACGAC TGTGCTGCCC 
CTCTACCAGA AAAGCGTGTG GACCCAGGAA TCCAAGTGCC ACCTGATTGA GACCAACATC 
AGGGACCAGG AGGAGCTGAA GGGCAAGAAG GTGCCCCAGT ACCCATGCCT GTGGGTCAAC 
GTGTCAGCTG CCGGCAGGTG GGCTGTGCTG TACCACACGG AGGACACTCG GGACCAGAAC 
CAGCAGTGCT CCTACATCCC AGGCAGCGTG GACAATTACC AGACGGCCCG GGCCGACGTG 
GAGAAGGTCA GAGCCAAATT CCAAGAGCAG CAGGTCTTCT ACTGCTTCTC CGCACCTCGG 
GGGAACGAAA CCAGCGTCCT ATTCCAGCGC CTCTACGGGC CCCAGGCCCT CCTCTTCTCC 
CTCTTCTGGC CCACCTTCCT GCTGACCGGT GGCCTCCTCA TTATCGCCAT GGTGAAGAGC 
AACCAGTACC TGTCCATCCT GGCGGCCCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNMB1 ( NM_004137.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004128.1 Homo sapiens potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), mRNA.

Price & Availability↑

CloneID	OHu19847
Clone ID Related Accession (Same CDS sequence)	NM_004137.3 , NM_004137.4
Accession Version	NM_004137.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	calcium-activated potassium channel subunit beta-1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC134820.1. On Nov 23, 2018 this sequence version replaced NM_004137.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U25138.1, SRR5189655.201403.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159368 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000274629.9/ ENSP00000274629.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTGAAGA AGCTGGTGAT GGCCCAGAAG CGGGGAGAGA CACGAGCCCT TTGCCTGGGT 
GTAACCATGG TGGTGTGTGC CGTCATCACC TACTACATCC TGGTCACGAC TGTGCTGCCC 
CTCTACCAGA AAAGCGTGTG GACCCAGGAA TCCAAGTGCC ACCTGATTGA GACCAACATC 
AGGGACCAGG AGGAGCTGAA GGGCAAGAAG GTGCCCCAGT ACCCATGCCT GTGGGTCAAC 
GTGTCAGCTG CCGGCAGGTG GGCTGTGCTG TACCACACGG AGGACACTCG GGACCAGAAC 
CAGCAGTGCT CCTACATCCC AGGCAGCGTG GACAATTACC AGACGGCCCG GGCCGACGTG 
GAGAAGGTCA GAGCCAAATT CCAAGAGCAG CAGGTCTTCT ACTGCTTCTC CGCACCTCGG 
GGGAACGAAA CCAGCGTCCT ATTCCAGCGC CTCTACGGGC CCCAGGCCCT CCTCTTCTCC 
CTCTTCTGGC CCACCTTCCT GCTGACCGGT GGCCTCCTCA TTATCGCCAT GGTGAAGAGC 
AACCAGTACC TGTCCATCCT GGCGGCCCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004128.1
CDS	134..709
Translation	MVKKLVMAQKRGETRALCLGVTMVVCAVITYYILVTTVLPLYQKSVWTQESKCHLIETNIRDQEELKGKKVPQYPCLWVNVSAAGRWAVLYHTEDTRDQNQQCSYIPGSVDNYQTARADVEKVRAKFQEQQVFYCFSAPRGNETSVLFQRLYGPQALLFSLFWPTFLLTGGLLIIAMVKSNQYLSILAAQK

Target ORF information:

RefSeq Version	NM_004137.4
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium calcium-activated channel subfamily M regulatory beta subunit 1 (KCNMB1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004137.4

ORF Insert Sequence:

ATGGTGAAGA AGCTGGTGAT GGCCCAGAAG CGGGGAGAGA CACGAGCCCT TTGCCTGGGT 
GTAACCATGG TGGTGTGTGC CGTCATCACC TACTACATCC TGGTCACGAC TGTGCTGCCC 
CTCTACCAGA AAAGCGTGTG GACCCAGGAA TCCAAGTGCC ACCTGATTGA GACCAACATC 
AGGGACCAGG AGGAGCTGAA GGGCAAGAAG GTGCCCCAGT ACCCATGCCT GTGGGTCAAC 
GTGTCAGCTG CCGGCAGGTG GGCTGTGCTG TACCACACGG AGGACACTCG GGACCAGAAC 
CAGCAGTGCT CCTACATCCC AGGCAGCGTG GACAATTACC AGACGGCCCG GGCCGACGTG 
GAGAAGGTCA GAGCCAAATT CCAAGAGCAG CAGGTCTTCT ACTGCTTCTC CGCACCTCGG 
GGGAACGAAA CCAGCGTCCT ATTCCAGCGC CTCTACGGGC CCCAGGCCCT CCTCTTCTCC 
CTCTTCTGGC CCACCTTCCT GCTGACCGGT GGCCTCCTCA TTATCGCCAT GGTGAAGAGC 
AACCAGTACC TGTCCATCCT GGCGGCCCAG AAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Regulation of vascular large-conductance calcium-activated potassium channels by Nrf2 signalling.
Diabetes & vascular disease research14(4)353-362(2017 Jul)
Li Y,Wang XL,Sun X,Chai Q,Li J,Thompson B,Shen WK,Lu T,Lee HC

The unique N-terminal sequence of the BKCa channel ?-subunit determines its modulation by ?-subunits.
PloS one12(7)e0182068(2017)
Lorca RA,Ma X,England SK

?1-subunit-induced structural rearrangements of the Ca2+- and voltage-activated K+ (BK) channel.
Proceedings of the National Academy of Sciences of the United States of America113(23)E3231-9(2016 Jun)
Castillo JP,S?nchez-Rodr?guez JE,Hyde HC,Zaelzer CA,Aguayo D,Sep?lveda RV,Luk LY,Kent SB,Gonzalez-Nilo FD,Bezanilla F,Latorre R

Molecular mechanism underlying ?1 regulation in voltage- and calcium-activated potassium (BK) channels.
Proceedings of the National Academy of Sciences of the United States of America112(15)4809-14(2015 Apr)
Castillo K,Contreras GF,Pupo A,Torres YP,Neely A,Gonz?lez C,Latorre R

Two distinct effects of PIP2 underlie auxiliary subunit-dependent modulation of Slo1 BK channels.
The Journal of general physiology145(4)331-43(2015 Apr)
Tian Y,Ullrich F,Xu R,Heinemann SH,Hou S,Hoshi T

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KCNMB1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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