KCNJ3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	KCNJ3
Entrez Gene ID	3760
Full Name	potassium voltage-gated channel subfamily J member 3
Synonyms	GIRK1,KGA,KIR3.1
General protein information	Preferred Names potassium voltage-gated channel subfamily J member 3 Names G protein-activated inward rectifier potassium channel 1 GIRK-1 inward rectifier K(+) channel Kir3.1 inward rectifier K+ channel KIR3.1 potassium channel, inwardly rectifying subfamily J member 3 potassium inwardly-rectifying channel subfamily J member 3 splice variant 1e potassium inwardly-rectifying channel, subfamily J, member 3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q24.1
Summary	Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and plays an important role in regulating heartbeat. It associates with three other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex that also couples to neurotransmitter receptors in the brain and whereby channel activation can inhibit action potential firing by hyperpolarizing the plasma membrane. These multimeric G-protein-gated inwardly-rectifying potassium (GIRK) channels may play a role in the pathophysiology of epilepsy, addiction, Down's syndrome, ataxia, and Parkinson's disease. Alternative splicing results in multiple transcript variants encoding distinct proteins. [provided by RefSeq, May 2012].
Disorder MIM:	601534

mRNA and Protein(s)

mRNA	Protein	Name
NM_001260509.1	NP_001247438.1	G protein-activated inward rectifier potassium channel 1 isoform 3
NM_001260510.2	NP_001247439.1	G protein-activated inward rectifier potassium channel 1 isoform 4
NM_001260510.1	NP_001247439.1	G protein-activated inward rectifier potassium channel 1 isoform 4
NM_002239.4	NP_002230.1	G protein-activated inward rectifier potassium channel 1 isoform 1
NM_001260508.1	NP_001247437.1	G protein-activated inward rectifier potassium channel 1 isoform 2
NM_001260509.2	NP_001247438.1	G protein-activated inward rectifier potassium channel 1 isoform 3
NM_002239.3	NP_002230.1	G protein-activated inward rectifier potassium channel 1 isoform 1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	KCNJ3	XP_545477.2
Rattus norvegicus (Norway rat)	Kcnj3	NP_113798.1
Mus musculus (house mouse)	Kcnj3	NP_032452.1
Bos taurus (cattle)	KCNJ3	NP_001179553.1
Gallus gallus (chicken)	KCNJ3	NP_990735.1
Danio rerio (zebrafish)	si:ch73-83c10.1	XP_002663356.2
Xenopus tropicalis (tropical clawed frog)	kcnj3	XP_002936698.1
Homo sapiens (human)	KCNJ3	NP_002230.1
	KCNJ3	XP_001084969.1

Gene Ontology
Bibliography

Related articles in PubMed

KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients.
Kammerer S, Sokolowski A, Hackl H, Platzer D, Jahn SW, El-Heliebi A, Schwarzenbacher D, Stiegelbauer V, Pichler M, Rezania S, Fiegl H, Peintinger F, Regitnig P, Hoefler G, Schreibmayer W, Bauernhofer T
Oncotarget7(51)84705-84717(2016 Dec)

Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner.
Rezania S, Kammerer S, Li C, Steinecker-Frohnwieser B, Gorischek A, DeVaney TT, Verheyen S, Passegger CA, Tabrizi-Wizsy NG, Hackl H, Platzer D, Zarnani AH, Malle E, Jahn SW, Bauernhofer T, Schreibmayer W
BMC cancer16628(2016 08)

The GIRK1 subunit potentiates G protein activation of cardiac GIRK1/4 hetero-tetramers.
Touhara KK, Wang W, MacKinnon R
eLife5(2016 04)

Variations in potassium channel genes are associated with distinct trajectories of persistent breast pain after breast cancer surgery.
Langford DJ, Paul SM, West CM, Dunn LB, Levine JD, Kober KM, Dodd MJ, Miaskowski C, Aouizerat BE
Pain156(3)371-80(2015 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Results clearly corroborate that overexpression of GIRK1 protein exerts profound effects on wound healing, chemoinvasion and cellular motility in the MCF-7 breast cancer cell line suggesting a role to promote invasion and metastasis.
Title: Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner.

GIRK1/GIRK4 hetero-tetramers are not activated by Na+, but rather are in a permanent state of high responsiveness to G proteins beta-gamma, suggesting that the GIRK1 subunit functions like a GIRK4 subunit with Na+ permanently bound.
Title: The GIRK1 subunit potentiates G protein activation of cardiac GIRK1/4 hetero-tetramers.

The findings of this study suggest that variations in KCNJ3 genes are associated with both mild and severe persistent breast pain after breast cancer surgery.
Title: Variations in potassium channel genes are associated with distinct trajectories of persistent breast pain after breast cancer surgery.

For KCNJ3 rs7574878, individuals who were heterozygous or homozygous for the rare G allele (TT versus TG+ GG) had a 48% reduction in the odds of reporting preoperative breast pain.
Title: Variations in potassium channel genes are associated with breast pain in women prior to breast cancer surgery.

we show that Kir3.1, in the absence of trafficking partner subunits, can exit the endoplasmic reticulum (ER) and reach the Golgi (though not the plasma membrane)
Title: A novel, radiolabel-free pulse chase strategy to study Kir3 channel ontogeny.

The following KCNJ3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the KCNJ3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01264	NM_002239.4 Latest version!	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu01295	NM_001260508.1 Latest version!	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu09731	NM_001260510.2 Latest version!	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu10051	NM_001260509.2 Latest version!	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu09731	NM_001260510.1	Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu10051	NM_001260509.1	Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu01264	NM_002239.3	Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

KCNJ3 ( NM_002239.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002230.1 Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01264
Clone ID Related Accession (Same CDS sequence)	NM_002239.4 , NM_002239.3
Accession Version	NM_002239.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	G protein-activated inward rectifier potassium channel 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U50964.1 and AC093633.3. On Nov 22, 2018 this sequence version replaced NM_002239.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as GIRK1a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.54688.1, SRR1803613.164476.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000295101.3/ ENSP00000295101.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG GATGACTTGT CAAGCTCGAA CATCATATAC TGAAGATGAA 
GTTCTTTGGG GTCATCGTTT TTTTCCTGTA ATTTCCTTAG AAGAGGGATT CTTTAAAGTT 
GATTACTCCC AGTTCCATGC AACATTTGAA GTCCCCACCC CACCTTACAG TGTGAAAGAG 
CAGGAGGAAA TGCTTCTCAT GTCGTCCCCT TTAATAGCAC CAGCCATAAC TAACAGCAAA 
GAAAGACATA ATTCTGTGGA ATGCTTAGAT GGACTAGATG ATATTACTAC AAAACTACCA 
TCTAAGCTGC AGAAAATTAC TGGAAGAGAA GACTTTCCCA AAAAACTCTT GAGGATGAGT 
TCTACAACTT CAGAAAAAGC CTACAGCTTG GGAGACTTGC CCATGAAACT TCAACGAATA 
AGTTCAGTTC CGGGCAACTC AGAAGAAAAA CTGGTATCTA AAACCACCAA GATGTTATCT 
GATCCCATGA GCCAGTCTGT GGCTGATTTG CCACCAAAGC TTCAAAAGAT GGCTGGAGGA 
GCAGCTAGGA TGGAAGGGAA CCTTCCAGCC AAATTAAGAA AAATGAACTC TGATCGCTTC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002230.1
CDS	82..1587
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDITTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGAARMEGNLPAKLRKMNSDRFT

Target ORF information:

RefSeq Version	NM_002239.4
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002239.4

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG GATGACTTGT CAAGCTCGAA CATCATATAC TGAAGATGAA 
GTTCTTTGGG GTCATCGTTT TTTTCCTGTA ATTTCCTTAG AAGAGGGATT CTTTAAAGTT 
GATTACTCCC AGTTCCATGC AACATTTGAA GTCCCCACCC CACCTTACAG TGTGAAAGAG 
CAGGAGGAAA TGCTTCTCAT GTCGTCCCCT TTAATAGCAC CAGCCATAAC TAACAGCAAA 
GAAAGACATA ATTCTGTGGA ATGCTTAGAT GGACTAGATG ATATTACTAC AAAACTACCA 
TCTAAGCTGC AGAAAATTAC TGGAAGAGAA GACTTTCCCA AAAAACTCTT GAGGATGAGT 
TCTACAACTT CAGAAAAAGC CTACAGCTTG GGAGACTTGC CCATGAAACT TCAACGAATA 
AGTTCAGTTC CGGGCAACTC AGAAGAAAAA CTGGTATCTA AAACCACCAA GATGTTATCT 
GATCCCATGA GCCAGTCTGT GGCTGATTTG CCACCAAAGC TTCAAAAGAT GGCTGGAGGA 
GCAGCTAGGA TGGAAGGGAA CCTTCCAGCC AAATTAAGAA AAATGAACTC TGATCGCTTC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNJ3 ( NM_002239.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002230.1 Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01264
Clone ID Related Accession (Same CDS sequence)	NM_002239.4 , NM_002239.3
Accession Version	NM_002239.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1506bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	G protein-activated inward rectifier potassium channel 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U50964.1 and AC093633.3. On May 9, 2012 this sequence version replaced NM_002239.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as GIRK1a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U50964.1, SRR1660803.54688.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG GATGACTTGT CAAGCTCGAA CATCATATAC TGAAGATGAA 
GTTCTTTGGG GTCATCGTTT TTTTCCTGTA ATTTCCTTAG AAGAGGGATT CTTTAAAGTT 
GATTACTCCC AGTTCCATGC AACATTTGAA GTCCCCACCC CACCTTACAG TGTGAAAGAG 
CAGGAGGAAA TGCTTCTCAT GTCGTCCCCT TTAATAGCAC CAGCCATAAC TAACAGCAAA 
GAAAGACATA ATTCTGTGGA ATGCTTAGAT GGACTAGATG ATATTACTAC AAAACTACCA 
TCTAAGCTGC AGAAAATTAC TGGAAGAGAA GACTTTCCCA AAAAACTCTT GAGGATGAGT 
TCTACAACTT CAGAAAAAGC CTACAGCTTG GGAGACTTGC CCATGAAACT TCAACGAATA 
AGTTCAGTTC CGGGCAACTC AGAAGAAAAA CTGGTATCTA AAACCACCAA GATGTTATCT 
GATCCCATGA GCCAGTCTGT GGCTGATTTG CCACCAAAGC TTCAAAAGAT GGCTGGAGGA 
GCAGCTAGGA TGGAAGGGAA CCTTCCAGCC AAATTAAGAA AAATGAACTC TGATCGCTTC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002230.1
CDS	196..1701
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEIVVILEGIVETTGMTCQARTSYTEDEVLWGHRFFPVISLEEGFFKVDYSQFHATFEVPTPPYSVKEQEEMLLMSSPLIAPAITNSKERHNSVECLDGLDDITTKLPSKLQKITGREDFPKKLLRMSSTTSEKAYSLGDLPMKLQRISSVPGNSEEKLVSKTTKMLSDPMSQSVADLPPKLQKMAGGAARMEGNLPAKLRKMNSDRFT

Target ORF information:

RefSeq Version	NM_002239.3
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002239.3

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG GATGACTTGT CAAGCTCGAA CATCATATAC TGAAGATGAA 
GTTCTTTGGG GTCATCGTTT TTTTCCTGTA ATTTCCTTAG AAGAGGGATT CTTTAAAGTT 
GATTACTCCC AGTTCCATGC AACATTTGAA GTCCCCACCC CACCTTACAG TGTGAAAGAG 
CAGGAGGAAA TGCTTCTCAT GTCGTCCCCT TTAATAGCAC CAGCCATAAC TAACAGCAAA 
GAAAGACATA ATTCTGTGGA ATGCTTAGAT GGACTAGATG ATATTACTAC AAAACTACCA 
TCTAAGCTGC AGAAAATTAC TGGAAGAGAA GACTTTCCCA AAAAACTCTT GAGGATGAGT 
TCTACAACTT CAGAAAAAGC CTACAGCTTG GGAGACTTGC CCATGAAACT TCAACGAATA 
AGTTCAGTTC CGGGCAACTC AGAAGAAAAA CTGGTATCTA AAACCACCAA GATGTTATCT 
GATCCCATGA GCCAGTCTGT GGCTGATTTG CCACCAAAGC TTCAAAAGAT GGCTGGAGGA 
GCAGCTAGGA TGGAAGGGAA CCTTCCAGCC AAATTAAGAA AAATGAACTC TGATCGCTTC 
ACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNJ3 ( NM_001260508.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247437.1 Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01295
Clone ID Related Accession (Same CDS sequence)	NM_001260508.1
Accession Version	NM_001260508.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Homo sapiens(human)
Product	G protein-activated inward rectifier potassium channel 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U50964.1, AK293824.1 and AC093633.3. Transcript Variant: This variant (2) lacks an exon in the coding region, compared to variant 1. This difference results in a frameshift and a protein (isoform 2; also known as GIRK1d) with a truncated C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.184736.1, SRR1803615.108770.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247437.1
CDS	196..903
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKG

Target ORF information:

RefSeq Version	NM_001260508.1
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260508.1

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AAGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNJ3 ( NM_001260510.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247439.1 Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu09731
Clone ID Related Accession (Same CDS sequence)	NM_001260510.2 , NM_001260510.1
Accession Version	NM_001260510.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	G protein-activated inward rectifier potassium channel 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U50964.1 and GU074516.1. On May 31, 2019 this sequence version replaced NM_001260510.1. Transcript Variant: This variant (4) lacks multiple 3' terminal exons and contains an additional coding segment, compared to variant 1. These differences result in a protein (isoform 4; also known as GIRK1e) with a truncated and novel C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript is intronless :: GU074516.1 [ECO:0000345] ##Evidence-Data-END##

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AAGTAAGTGC TCCCCGCCCC 
TTCCCCACCG GGAGACCTGC GTCCCCCAAA CCCGCGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247439.1
CDS	82..843
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKVSAPRPFPTGRPASPKPAE

Target ORF information:

RefSeq Version	NM_001260510.2
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260510.2

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AAGTAAGTGC TCCCCGCCCC 
TTCCCCACCG GGAGACCTGC GTCCCCCAAA CCCGCGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNJ3 ( NM_001260510.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247439.1 Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu09731
Clone ID Related Accession (Same CDS sequence)	NM_001260510.2 , NM_001260510.1
Accession Version	NM_001260510.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	G protein-activated inward rectifier potassium channel 1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U50964.1 and GU074516.1. Transcript Variant: This variant (4) lacks multiple 3' terminal exons and contains an additional coding segment, compared to variant 1. These differences result in a protein (isoform 4; also known as GIRK1e) with a truncated and novel C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AAGTAAGTGC TCCCCGCCCC 
TTCCCCACCG GGAGACCTGC GTCCCCCAAA CCCGCGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247439.1
CDS	196..957
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKVSAPRPFPTGRPASPKPAE

Target ORF information:

RefSeq Version	NM_001260510.1
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260510.1

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AAGTAAGTGC TCCCCGCCCC 
TTCCCCACCG GGAGACCTGC GTCCCCCAAA CCCGCGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNJ3 ( NM_001260509.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247438.1 Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10051
Clone ID Related Accession (Same CDS sequence)	NM_001260509.1 , NM_001260509.2
Accession Version	NM_001260509.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	G protein-activated inward rectifier potassium channel 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U50964.1 and GU068048.1. Transcript Variant: This variant (3) lacks the 3' terminal exon and uses an alternate splice site in the upstream coding region, compared to variant 1. These differences result in a protein (isoform 3; also known as GIRK1c) with a truncated and novel C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU068048.1, BG702790.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247438.1
CDS	196..1122
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEIVVILEGIVETTGE

Target ORF information:

RefSeq Version	NM_001260509.1
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium voltage-gated channel subfamily J member 3 (KCNJ3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260509.1

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNJ3 ( NM_001260509.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001247438.1 Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu10051
Clone ID Related Accession (Same CDS sequence)	NM_001260509.1 , NM_001260509.2
Accession Version	NM_001260509.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 927bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	G protein-activated inward rectifier potassium channel 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U50964.1 and GU068048.1. On May 31, 2019 this sequence version replaced NM_001260509.1. Transcript Variant: This variant (3) lacks the 3' terminal exon and uses an alternate splice site in the upstream coding region, compared to variant 1. These differences result in a protein (isoform 3; also known as GIRK1c) with a truncated and novel C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: GU068048.1, SRR1660807.294920.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END##

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001247438.1
CDS	82..1008
Translation	MSALRRKFGDDYQVVTTSSSGSGLQPQGPGQDPQQQLVPKKKRQRFVDKNGRCNVQHGNLGSETSRYLSDLFTTLVDLKWRWNLFIFILTYTVAWLFMASMWWVIAYTRGDLNKAHVGNYTPCVANVYNFPSAFLFFIETEATIGYGYRYITDKCPEGIILFLFQSILGSIVDAFLIGCMFIKMSQPKKRAETLMFSEHAVISMRDGKLTLMFRVGNLRNSHMVSAQIRCKLLKSRQTPEGEFLPLDQLELDVGFSTGADQLFLVSPLTICHVIDAKSPFYDLSQRSMQTEQFEIVVILEGIVETTGE

Target ORF information:

RefSeq Version	NM_001260509.2
Organism	Homo sapiens(human)
Definition	Homo sapiens potassium inwardly rectifying channel subfamily J member 3 (KCNJ3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001260509.2

ORF Insert Sequence:

ATGTCTGCAC TCCGAAGGAA ATTTGGGGAC GATTATCAGG TAGTGACCAC ATCGTCCAGC 
GGCTCGGGCT TGCAGCCCCA GGGGCCAGGC CAGGACCCTC AGCAGCAGCT TGTGCCCAAG 
AAGAAGCGGC AGCGGTTCGT GGACAAGAAC GGCCGGTGCA ATGTACAGCA CGGCAACCTG 
GGCAGCGAGA CAAGCCGCTA CCTCTCGGAC CTCTTCACCA CGCTGGTGGA CCTCAAGTGG 
CGCTGGAACC TCTTCATCTT CATTCTCACC TACACCGTGG CCTGGCTTTT CATGGCGTCC 
ATGTGGTGGG TGATCGCCTA CACTCGGGGC GACCTGAACA AAGCCCACGT CGGTAACTAC 
ACGCCTTGCG TGGCCAATGT CTATAACTTC CCTTCTGCCT TCCTCTTCTT CATCGAGACG 
GAGGCCACCA TCGGCTATGG CTACCGATAC ATCACAGACA AGTGCCCCGA GGGCATCATC 
CTCTTCCTCT TCCAGTCCAT CCTGGGCTCC ATCGTGGACG CCTTCCTCAT CGGCTGCATG 
TTCATCAAGA TGTCCCAGCC CAAGAAGCGC GCCGAGACCC TCATGTTCAG CGAGCACGCG 
GTGATCTCCA TGAGGGACGG AAAACTCACG CTTATGTTCC GGGTGGGCAA CCTGCGCAAC 
AGCCACATGG TCTCCGCGCA GATTCGCTGC AAGCTGCTCA AATCTCGGCA GACACCTGAG 
GGTGAGTTCC TTCCCCTTGA CCAACTTGAA CTGGATGTAG GTTTTAGTAC AGGGGCAGAT 
CAACTTTTTC TTGTGTCCCC CCTCACAATT TGCCACGTGA TCGATGCCAA AAGCCCCTTT 
TATGACCTAT CCCAGCGAAG CATGCAAACT GAACAGTTCG AGATTGTCGT CATCCTAGAA 
GGCATTGTGG AAACAACTGG TGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

KCNJ3 is a new independent prognostic marker for estrogen receptor positive breast cancer patients.
Oncotarget7(51)84705-84717(2016 Dec)
Kammerer S,Sokolowski A,Hackl H,Platzer D,Jahn SW,El-Heliebi A,Schwarzenbacher D,Stiegelbauer V,Pichler M,Rezania S,Fiegl H,Peintinger F,Regitnig P,Hoefler G,Schreibmayer W,Bauernhofer T

Overexpression of KCNJ3 gene splice variants affects vital parameters of the malignant breast cancer cell line MCF-7 in an opposing manner.
BMC cancer16628(2016 08)
Rezania S,Kammerer S,Li C,Steinecker-Frohnwieser B,Gorischek A,DeVaney TT,Verheyen S,Passegger CA,Tabrizi-Wizsy NG,Hackl H,Platzer D,Zarnani AH,Malle E,Jahn SW,Bauernhofer T,Schreibmayer W

The GIRK1 subunit potentiates G protein activation of cardiac GIRK1/4 hetero-tetramers.
eLife5(2016 04)
Touhara KK,Wang W,MacKinnon R

Variations in potassium channel genes are associated with distinct trajectories of persistent breast pain after breast cancer surgery.
Pain156(3)371-80(2015 Mar)
Langford DJ,Paul SM,West CM,Dunn LB,Levine JD,Kober KM,Dodd MJ,Miaskowski C,Aouizerat BE

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KCNJ3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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