MAST4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MAST4
Entrez Gene ID	375449
Full Name	microtubule associated serine/threonine kinase family member 4
General protein information	Preferred Names microtubule associated serine/threonine kinase family member 4 Names microtubule-associated serine/threonine-protein kinase 4
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q12.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001164664.1	NP_001158136.1	microtubule-associated serine/threonine-protein kinase 4 isoform c
NM_198828.3	NP_942123.1	microtubule-associated serine/threonine-protein kinase 4 isoform b
NM_001297651.2	NP_001284580.1	microtubule-associated serine/threonine-protein kinase 4 isoform g
NM_015183.3	NP_055998.1	microtubule-associated serine/threonine-protein kinase 4 isoform a
NM_001290226.1	NP_001277155.1	microtubule-associated serine/threonine-protein kinase 4 isoform d
NM_198828.2	NP_942123.1	microtubule-associated serine/threonine-protein kinase 4 isoform b
XM_011543382.2	XP_011541684.1	microtubule-associated serine/threonine-protein kinase 4 isoform X2
XM_011543385.2	XP_011541687.1	microtubule-associated serine/threonine-protein kinase 4 isoform X8
NM_001290228.2	NP_001277157.1	microtubule-associated serine/threonine-protein kinase 4 isoform f
NM_001290227.2	NP_001277156.1	microtubule-associated serine/threonine-protein kinase 4 isoform e
XM_017009449.1	XP_016864938.1	microtubule-associated serine/threonine-protein kinase 4 isoform X2
XM_011543384.2	XP_011541686.1	microtubule-associated serine/threonine-protein kinase 4 isoform X4
XM_017009450.1	XP_016864939.1	microtubule-associated serine/threonine-protein kinase 4 isoform X5
NM_015183.2	NP_055998.1	microtubule-associated serine/threonine-protein kinase 4 isoform a
XM_011543385.3	XP_011541687.1	microtubule-associated serine/threonine-protein kinase 4 isoform X6
XM_017009451.1	XP_016864940.1	microtubule-associated serine/threonine-protein kinase 4 isoform X9
XM_017009452.1	XP_016864941.1	microtubule-associated serine/threonine-protein kinase 4 isoform X10
NM_001290226.2	NP_001277155.1	microtubule-associated serine/threonine-protein kinase 4 isoform d
NM_001164664.2	NP_001158136.1	microtubule-associated serine/threonine-protein kinase 4 isoform c
XM_011543386.2	XP_011541688.1	microtubule-associated serine/threonine-protein kinase 4 isoform X7
XM_017009453.1	XP_016864942.1	microtubule-associated serine/threonine-protein kinase 4 isoform X10
NM_001290228.1	NP_001277157.1	microtubule-associated serine/threonine-protein kinase 4 isoform f
NM_001297651.1	NP_001284580.1	microtubule-associated serine/threonine-protein kinase 4 isoform g
NM_001290227.1	NP_001277156.1	microtubule-associated serine/threonine-protein kinase 4 isoform e
XM_017009448.1	XP_016864937.1	microtubule-associated serine/threonine-protein kinase 4 isoform X3
XM_017009447.1	XP_016864936.1	microtubule-associated serine/threonine-protein kinase 4 isoform X1
XM_024446044.1	XP_024301812.1	microtubule-associated serine/threonine-protein kinase 4 isoform X1
XM_006714606.3	XP_006714669.1	microtubule-associated serine/threonine-protein kinase 4 isoform X3
XM_006714610.2	XP_006714673.1	microtubule-associated serine/threonine-protein kinase 4 isoform X8

Pathways from BioSystems

Homologies

Homo sapiens (human)	MAST4	NP_001158136.1
Bos taurus (cattle)	MAST4	XP_003587555.2
Rattus norvegicus (Norway rat)	Mast4	XP_006231936.1
Gallus gallus (chicken)	MAST4	XP_004937359.1
Pan troglodytes (chimpanzee)	MAST4	XP_003950594.1
Mus musculus (house mouse)	Mast4	NP_780380.2
Canis lupus familiaris (dog)	MAST4	XP_005617968.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide association analysis of genetic generalized epilepsies implicates susceptibility loci at 1q43, 2p16.1, 2q22.3 and 17q21.32.
, , Steffens M, Leu C, Ruppert AK, Zara F, Striano P, Robbiano A, Capovilla G, Tinuper P, Gambardella A, Bianchi A, La Neve A, Crichiutti G, de Kovel CG, Kasteleijn-Nolst Trenit? D, de Haan GJ, Lindhout D, Gaus V, Schmitz B, Janz D, Weber YG, Becker F, Lerche H, Steinhoff BJ, Kleefu?-Lie AA, Kunz WS, Surges R, Elger CE, Muhle H, von Spiczak S, Ostertag P, Helbig I, Stephani U, M?ller RS, Hjalgrim H, Dibbens LM, Bellows S, Oliver K, Mullen S, Scheffer IE, Berkovic SF, Everett KV, Gardiner MR, Marini C, Guerrini R, Lehesjoki AE, Siren A, Guipponi M, Malafosse A, Thomas P, Nabbout R, Baulac S, Leguern E, Guerrero R, Serratosa JM, Reif PS, Rosenow F, M?rzinger M, Feucht M, Zimprich F, Kapser C, Schankin CJ, Suls A, Smets K, De Jonghe P, Jordanova A, Caglayan H, Yapici Z, Yalcin DA, Baykan B, Bebek N, Ozbek U, Gieger C, Wichmann HE, Balschun T, Ellinghaus D, Franke A, Meesters C, Becker T, Wienker TF, Hempelmann A, Schulz H, R?schendorf F, Leber M, Pauck SM, Trucks H, Toliat MR, N?rnberg P, Avanzini G, Koeleman BP, Sander T
Human molecular genetics21(24)5359-72(2012 Dec)

Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.
Martins-de-Souza D, Guest PC, Mann DM, Roeber S, Rahmoune H, Bauder C, Kretzschmar H, Volk B, Baborie A, Bahn S
Journal of proteome research11(4)2533-43(2012 Apr)

A genome-wide association study of carotid atherosclerosis in HIV-infected men.
Shrestha S, Irvin MR, Taylor KD, Wiener HW, Pajewski NM, Haritunians T, Delaney JA, Schambelan M, Polak JF, Arnett DK, Chen YD, Grunfeld C
AIDS (London, England)24(4)583-92(2010 Feb)

[Identification of a novel human MAST4 gene, a new member of the microtubule associated serine-threonine kinase family].
Sun L, Gu S, Li X, Sun Y, Zheng D, Yu K, Ji C, Tang R, Xie Y, Mao Y
Molekuliarnaia biologiia40(5)808-15(2006 Sep-Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A protein encoded by this locus was found to be differentially expressed in postmortem brains from patients with atypical frontotemporal lobar degeneration.
Title: Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

high expression level of MAST4 in most normal human tissues, with an exception of in testis, small intestine, colon and peripheral blood leukocyte.
Title: [Identification of a novel human MAST4 gene, a new member of the microtubule associated serine-threonine kinase family].

The following MAST4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MAST4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13482	NM_198828.3 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu95870	XM_017009449.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu95871	XM_017009450.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu95872	XM_017009451.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OHu95873	XM_017009452.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu95873	XM_017009453.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
OHu14568	NM_015183.3 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu15116	NM_001290227.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu15141	NM_001290226.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu39568	NM_001297651.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu15116	XM_006714610.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu39568	XM_006714606.3 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu79755	XM_011543384.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu79756	XM_011543385.3 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu79757	XM_011543386.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu15018	NM_001290228.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu21594	NM_001164664.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu105665	XM_024446044.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu79753	XM_011543382.2 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
OHu95868	XM_017009447.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu95869	XM_017009448.1 Latest version!	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1301.30 $1859.00
View Old Accession Versions >>
OHu13482	NM_198828.2	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu14568	NM_015183.2	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$923.30 $1319.00
OHu79756	XM_011543385.2	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu15141	NM_001290226.1	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu21594	NM_001164664.1	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu15018	NM_001290228.1	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu39568	NM_001297651.1	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu15116	NM_001290227.1	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MAST4 ( NM_198828.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_942123.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13482
Clone ID Related Accession (Same CDS sequence)	NM_198828.3 , NM_198828.2
Accession Version	NM_198828.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC033215.1. On Jun 2, 2019 this sequence version replaced NM_198828.2. Transcript Variant: This variant (2) uses a distinct 3' coding region and 3' UTR, compared to variant 3. The resulting isoform (b) has a substantially shorter and distinct C-terminus, compared to isoform c. ##Evidence-Data-START## Transcript exon combination :: BC033215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03568912 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTGATAGA TTCCCAGAAG TGGAATTGCT TGGTCAAACG CCCTGTGTGT 
CCAAATGCTG GGAGAACATC ACCCCTTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942123.1
CDS	264..1016
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSLIDSQKWNCLVKRPVCPNAGRTSPLG

Target ORF information:

RefSeq Version	NM_198828.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198828.3

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTGATAGA TTCCCAGAAG TGGAATTGCT TGGTCAAACG CCCTGTGTGT 
CCAAATGCTG GGAGAACATC ACCCCTTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_198828.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_942123.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu13482
Clone ID Related Accession (Same CDS sequence)	NM_198828.3 , NM_198828.2
Accession Version	NM_198828.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 753bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-20
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM967134.1 and BC033215.1. On Jun 8, 2007 this sequence version replaced NM_198828.1. Transcript Variant: This variant (2) uses a distinct 3' coding region and 3' UTR, compared to variant 3. The resulting isoform (b) has a substantially shorter and distinct C-terminus, compared to isoform c. ##Evidence-Data-START## Transcript exon combination :: BC033215.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03568912 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTGATAGA TTCCCAGAAG TGGAATTGCT TGGTCAAACG CCCTGTGTGT 
CCAAATGCTG GGAGAACATC ACCCCTTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942123.1
CDS	309..1061
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSLIDSQKWNCLVKRPVCPNAGRTSPLG

Target ORF information:

RefSeq Version	NM_198828.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198828.2

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTGATAGA TTCCCAGAAG TGGAATTGCT TGGTCAAACG CCCTGTGTGT 
CCAAATGCTG GGAGAACATC ACCCCTTGGA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_017009449.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864938.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu95870
Clone ID Related Accession (Same CDS sequence)	XM_017009449.1
Accession Version	XM_017009449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7326bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCCAGG 
AGCCTGAGTC CAACCCCATC CAGCCCAGGC AGTCCTTGTA GTCCTCTCTT GGCCTTTCAC 
TTTTGGAGTT GCCGAACAAG CAACCGGAAA AGCTTAATAG GCAATGGGCA GTCACCAGCA 
TTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA 
AGAAATTTCT CCCCCAGTGC CTCAGCCCAT TTTTCATTTG CACGGAGGAC TGATGGACGC 
CGCTGGTCGT TGGCTTCTCT CCCTTCCTCT GGCTATGGGA CAAACACACC CAGCTCTACG 
GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCATACCA ACCAACACCA 
GACGAGTTAC ACTTCTTATC AAAACATTTC TGTACCACCG AAAGCATCGC CACTGAGAAC 
AGATGCAGGA ACACGCCGAT GCGCCCCCGT TCCCGAAGTC TGAGCCCTGG ACGTTCTCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCTACAGCTC AGATGGAAGA ACGTCTAAAG GAAATTATCA CCAGCTACTC TCCTGACAAC 
GTTCTACCCT TAGCAGATGG AGTGCTTAGT TTCACTCACC ACCAGATTAT TGAACTGGCT 
CGAGATTGCT TGGATAAATC CCACCAGGGC CTCATCACCT CACGATACTT CCTTGAATTA 
CAGCACAAAT TAGATAAGTT GCTACAGGAG GCTCATGATC GTTCAGAAAG TGGAGAATTG 
GCATTTATTA AACAACTAGT TCGAAAGATC CTAATTGTTA TTGCCCGCCC TGCTCGGTTA 
TTAGAGTGCC TGGAATTTGA TCCGGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC 
CATGCCAAAG AAGGACAGGG TATTAAAACC GACATTCCCA GGTACATCAT TAGCCAACTG 
GGACTCAATA AGGATCCCTT GGAAGAAATG GCTCATTTGG GAAACTACGA TAGTGGGACA 
GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCCTCCCT GAAACTTCGA 
AGGAAACCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TTAGCAATGG AGCCTATGGG 
GCAGTCTACT TTGTTCGGCA TAAAGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAT 
AAACAGAACC TCATCCTTCG AAACCAGATC CAGCAGGCCT TTGTGGAGCG GGATATCCTG 
ACTTTTGCAG AAAACCCCTT TGTTGTCAGC ATGTATTGCT CCTTTGAAAC AAGGCGCCAC 
TTGTGCATGG TCATGGAATA TGTGGAAGGG GGAGACTGTG CTACTTTAAT GAAAAACATG 
GGTCCTCTCC CTGTTGATAT GGCCAGAATG TACTTTGCTG AGACGGTCTT GGCCTTGGAA 
TATTTACATA ATTATGGAAT TGTACACAGG GATTTGAAAC CAGACAACTT GTTGGTTACC 
TCCATGGGGC ACATAAAGCT GACAGATTTT GGATTATCTA AGGTGGGACT AATGAGCATG 
ACTACCAACC TTTACGAGGG TCATATTGAG AAGGATGCTA GAGAGTTCCT GGATAAACAG 
GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG TTATGGAAAG 
CCGGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA 
TTCTTTGGGG ATACTCCAGA GGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG 
CCTGAGAAGG ATGAGGCACC CCCACCTGAT GCCCAGGATC TGATTACCTT ACTCCTCAGG 
CAGAATCCCC TGGAGAGGCT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC 
TTCCGTTCTT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTTAT TCCCCAACTG 
GAATCTGAGG ATGACACAAG TTATTTTGAT ACTCGGTCTG AGAAGTATCA TCATATGGAA 
ACGGAGGAAG AAGATGACAC AAATGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCT 
TCATGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGAATCAC TCAGAATTCA 
GCAGAAGAGA AGGAAGACTC TGTGGACAAA ACCAAAAGCA CCACCTTGCC ATCCACAGAA 
ACACTGAGCT GGAGTTCAGA ATATTCTGAA ATGCAACAGC TATCAACATC CAACTCTTCA 
GATACTGAAA GCAACAGACA TAAACTCAGT TCTGGCCTAC TTCCCAAACT GGCTATTTCA 
ACAGAGGGAG AGCAAGATGA AGCTGCCTCC TGCCCTGGAG ACCCCCATGA GGAGCCAGGA 
AAGCCAGCCC TTCCTCCTGA AGAGTGTGCC CAGGAGGAGC CTGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGCTCCAC CCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGACGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
GCTTCTCACA TGGCTCGGCA GCGATTAGAA AGCACAGAAA AAAAGAAAAT CTCGGGGAAA 
GTCACAAAGT CCCTCTCTGC CAGTGCTCTT TCCCTCATGA TCCCAGGAGA TATGTTTGCT 
GTTTCCCCTC TGGGAAGTCC AATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCTTCA 
CGAGATTCCT CTCCCAGCCG AGATTCCTCA GCAGCTTCTG CCAGTCCACA TCAGCCGATT 
GTGATCCACA GTTCGGGGAA GAACTACGGC TTTACCATCC GAGCCATCCG GGTGTATGTG 
GGAGACAGTG ACATCTATAC AGTGCACCAT ATCGTCTGGA ATGTAGAAGA AGGAAGTCCG 
GCATGCCAGG CAGGACTGAA GGCTGGAGAT CTTATCACTC ACATCAATGG AGAACCAGTG 
CATGGACTTG TCCACACAGA AGTTATAGAA CTCCTACTGA AGAGTGGGAA TAAGGTGTCA 
ATCACTACTA CCCCATTTGA AAACACATCA ATCAAAACTG GACCAGCCAG GAGAAACAGC 
TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCCAAGA AGAAAGAAAG TCTCGAAAGG 
AGGAGATCTC TTTTCAAAAA GCTAGCCAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA 
AGTTTCTCCT GCTTGAACAG ATCCCTGTCA TCGGGTGAGA GCCTCCCAGG TTCCCCCACT 
CATAGCTTGT CTCCCCGGTC TCCAACACCA AGCTACCGCT CCACCCCTGA CTTCCCATCT 
GGTACTAATT CCTCCCAGAG CAGCTCCCCT AGTTCTAGTG CCCCCAATTC CCCAGCAGGG 
TCCGGGCACA TCCGGCCCAG CACTCTCCAC GGTCTTGCAC CCAAACTCGG CGGGCAGCGG 
TACCGGTCCG GAAGGCGAAA GTCCGCCGGC AACATCCCAC TGTCCCCGCT GGCCCGGACG 
CCCTCTCCAA CCCCGCAACC CACCTCCCCG CAGCGGTCAC CATCCCCTCT TCTGGGACAC 
TCACTGGGCA ATTCCAAGAT CGCGCAAGCC TTTCCCAGCA AGATGCACTC CCCGCCCACC 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCGGAGCCCC CCAGGTCCCC GCTGCTCAAG 
CGCGTGCAGT CCGAGGAGAA GCTGTCGCCC TCTTACGGCA GTGACAAGAA GCACCTGTGC 
TCCCGCAAGC ACAGCCTGGA GGTGACCCAA GAGGAGGTGC AGCGGGAGCA GTCCCAGCGG 
GAGGCGCCGC TGCAGAGCCT GGATGAGAAC GTGTGCGACG TGCCGCCGCT CAGCCGCGCC 
CGGCCAGTGG AGCAAGGCTG CCTGAAACGC CCAGTCTCCC GGAAGGTGGG CCGCCAGGAG 
TCTGTGGACG ACCTGGACCG CGACAAGCTG AAGGCCAAGG TGGTGGTGAA GAAAGCAGAC 
GGCTTCCCAG AGAAACAGGA ATCCCACCAG AAATCCCATG GACCCGGGAG TGATTTGGAA 
AACTTTGCTC TGTTTAAGCT GGAAGAGAGA GAGAAGAAAG TCTATCCGAA GGCTGTGGAA 
AGGTCAAGTA CTTTTGAAAA CAAAGCGTCT ATGCAGGAGG CGCCACCGCT GGGCAGCCTG 
CTGAAGGATG CTCTTCACAA GCAGGCCAGC GTGCGCGCCA GCGAGGGTGC GATGTCGGAT 
GGCCGGGTGC CTGCGGAGCA CCGCCAGGGT GGCGGGGACT TCAGACGGGC CCCCGCTCCT 
GGCACCCTCC AGGATGGTCT CTGCCACTCC CTCGACAGGG GCATCTCTGG GAAGGGGGAA 
GGCACGGAGA AGTCCTCCCA GGCCAAGGAG CTTCTCCGAT GTGAAAAGTT AGACAGCAAG 
CTGGCCAACA TCGATTACCT CCGAAAGAAA ATGTCACTTG AGGACAAAGA GGACAACCTC 
TGCCCTGTGC TGAAGCCCAA GATGACAGCT GGCTCCCACG AATGCCTGCC AGGGAACCCA 
GTCCGACCCA CGGGTGGGCA GCAGGAGCCC CCGCCGGCTT CTGAGAGCCG AGCTTTTGTC 
AGCAGCACCC ATGCAGCTCA GATGAGTGCC GTCTCTTTTG TTCCCCTCAA GGCCTTAACA 
GGCCGGGTGG ACAGTGGAAC GGAGAAGCCT GGCTTGGTTG CTCCTGAGTC CCCTGTTAGG 
AAGAGCCCCT CCGAGTATAA GCTGGAAGGT AGGTCTGTCT CATGCCTGAA GCCGATCGAG 
GGCACTCTGG ACATTGCTCT CCTGTCCGGA CCTCAGGCCT CCAAGACAGA ACTGCCTTCC 
CCAGAGTCTG CACAGAGCCC CAGCCCAAGT GGTGACGTGA GGGCCTCTGT GCCACCAGTT 
CTCCCCAGCA GCAGTGGGAA AAAGAACGAT ACCACCAGTG CAAGAGAGCT TTCTCCTTCC 
AGCTTAAAGA TGAATAAATC CTACCTGCTG GAGCCTTGGT TCCTGCCCCC CAGCCGAGGT 
CTCCAGAATT CACCAGCAGT TTCCCTGCCT GACCCAGAGT TCAAGAGGGA CAGGAAAGGT 
CCCCATCCTA CTGCCAGGAG CCCTGGAACA GTCATGGAAA GCAATCCCCA ACAGAGAGAG 
GGCAGCTCCC CTAAACACCA AGACCACACC ACTGACCCCA AGCTTCTGAC CTGCCTGGGG 
CAGAACCTCC ACAGCCCTGA CCTGGCCAGG CCACGCTGCC CGCTCCCACC TGAAGCTTCC 
CCCTCAAGGG AGAAGCCAGG CCTGAGGGAA TCGTCTGAAA GAGGCCCTCC CACAGCCAGA 
AGCGAGCGCT CTGCTGCGAG GGCTGACACA TGCAGAGAGC CCTCCATGGA ACTGTGCTTT 
CCAGAAACTG CGAAAACCAG TGACAACTCC AAAAATCTCC TCTCTGTGGG AAGGACCCAC 
CCAGATTTCT ATACACAGAC CCAGGCCATG GAGAAAGCAT GGGCGCCGGG TGGGAAAACG 
AACCACAAAG ATGGCCCAGG TGAGGCGAGG CCCCCGCCCA GAGACAACTC CTCTCTGCAC 
TCAGCTGGAA TTCCCTGTGA GAAGGAGCTG GGCAAGGTGA GGCGTGGCGT GGAACCCAAG 
CCCGAAGCGC TTCTTGCCAG GCGGTCTCTG CAGCCACCTG GAATTGAGAG TGAGAAGAGT 
GAAAAGCTCT CCAGTTTCCC ATCTTTGCAG AAAGATGGTG CCAAGGAACC TGAAAGGAAG 
GAGCAGCCTC TACAAAGGCA TCCCAGCAGC ATCCCTCCGC CCCCTCTGAC GGCCAAAGAC 
CTGTCCAGCC CGGCTGCCAG GCAGCATTGC AGTTCCCCAA GCCACGCTTC TGGCAGAGAG 
CCGGGGGCCA AGCCCAGCAC TGCAGAGCCC AGCTCGAGCC CCCAGGACCC TCCCAAGCCT 
GTTGCTGCGC ACAGTGAAAG CAGCAGCCAC AAGCCCCGGC CTGGCCCTGA CCCGGGCCCT 
CCAAAGACTA AGCACCCCGA CCGGTCCCTC TCCTCTCAGA AACCAAGTGT CGGGGCCACA 
AAGGGCAAAG AGCCTGCCAC TCAGTCCCTC GGTGGCTCTA GCAGAGAGGG GAAGGGCCAC 
AGTAAGAGTG GGCCGGATGT GTTTCCTGCT ACCCCAGGCT CCCAGAACAA AGCCAGCGAT 
GGGATTGGCC AGGGAGAAGG TGGGCCCTCT GTCCCACTGC ACACTGACAG GGCTCCTCTA 
GACGCCAAGC CACAACCCAC CAGTGGTGGG CGGCCCCTGG AGGTGCTGGA GAAGCCTGTG 
CATTTGCCAA GGCCGGGACA CCCAGGGCCT AGTGAGCCAG CGGACCAGAA ACTGTCCGCT 
GTTGGTGAAA AGCAAACCCT GTCTCCAAAG CACCCCAAAC CATCCACTGT GAAAGATTGC 
CCCACCCTGT GCAAACAGAC AGACAACAGA CAGACAGACA AAAGCCCGAG TCAGCCGGCC 
GCCAACACCG ACAGAAGGGC GGAAGGGAAG AAATGCACTG AAGCACTTTA TGCTCCAGCA 
GAGGGCGACA AGCTCGAGGC CGGCCTTTCC TTTGTGCATA GCGAGAACCG GTTGAAAGGC 
GCGGAGCGGC CAGCCGCGGG GGTGGGGAAG GGCTTCCCTG AGGCCAGAGG GAAAGGGCCC 
GGTCCCCAGA AGCCACCGAC GGAGGCAGAC AAGCCCAATG GCATGAAACG GTCCCCCTCA 
GCCACTGGGC AGAGTTCTTT CCGATCCACG GCCCTCCCGG AAAAGTCTCT GAGCTGCTCC 
TCCAGCTTCC CTGAAACCAG GGCCGGAGTT AGAGAGGCCT CTGCAGCCAG CAGCGACACC 
TCTTCTGCCA AGGCCGCCGG GGGCATGCTG GAGCTTCCAG CCCCCAGCAA CAGGGACCAT 
AGGAAGGCTC AGCCTGCCGG GGAGGGCCGA ACCCACATGA CAAAGAGTGA CTCCCTGCCC 
TCCTTCCGGG TCTCCACCCT GCCTCTGGAG TCACACCACC CCGACCCAAA CACCATGGGC 
GGGGCCAGCC ACCGGGACAG GGCTCTCTCG GTGACTGCCA CCGTAGGGGA AACCAAAGGG 
AAGGACCCTG CCCCAGCCCA GCCTCCCCCA GCTAGGAAAC AGAACGTGGG CAGAGACGTG 
ACCAAGCCAT CCCCAGCCCC AAACACTGAC CGCCCCATCT CTCTTTCTAA TGAGAAGGAC 
TTTGTGGTAC GGCAGAGGCG GGGGAAAGAG AGTTTGCGTA GCAGCCCTCA CAAAAAGGCC 
TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864938.1
CDS	346..7671
Translation	MKAQRERLQIPGLTLDLTPRSLSPTPSSPGSPCSPLLAFHFWSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_017009449.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009449.1

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATCT CACTCCCAGG 
AGCCTGAGTC CAACCCCATC CAGCCCAGGC AGTCCTTGTA GTCCTCTCTT GGCCTTTCAC 
TTTTGGAGTT GCCGAACAAG CAACCGGAAA AGCTTAATAG GCAATGGGCA GTCACCAGCA 
TTGCCTCGAC CACACTCACC TCTCTCTGCT CATGCAGGAA ATAGCCCTCA AGATAGTCCA 
AGAAATTTCT CCCCCAGTGC CTCAGCCCAT TTTTCATTTG CACGGAGGAC TGATGGACGC 
CGCTGGTCGT TGGCTTCTCT CCCTTCCTCT GGCTATGGGA CAAACACACC CAGCTCTACG 
GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG TTGCATCAGT TACCATACCA ACCAACACCA 
GACGAGTTAC ACTTCTTATC AAAACATTTC TGTACCACCG AAAGCATCGC CACTGAGAAC 
AGATGCAGGA ACACGCCGAT GCGCCCCCGT TCCCGAAGTC TGAGCCCTGG ACGTTCTCCC 
GCCTGCTGTG ACCATGAAAT AATTATGATG AACCATGTCT ACAAAGAAAG GTTCCCAAAG 
GCTACAGCTC AGATGGAAGA ACGTCTAAAG GAAATTATCA CCAGCTACTC TCCTGACAAC 
GTTCTACCCT TAGCAGATGG AGTGCTTAGT TTCACTCACC ACCAGATTAT TGAACTGGCT 
CGAGATTGCT TGGATAAATC CCACCAGGGC CTCATCACCT CACGATACTT CCTTGAATTA 
CAGCACAAAT TAGATAAGTT GCTACAGGAG GCTCATGATC GTTCAGAAAG TGGAGAATTG 
GCATTTATTA AACAACTAGT TCGAAAGATC CTAATTGTTA TTGCCCGCCC TGCTCGGTTA 
TTAGAGTGCC TGGAATTTGA TCCGGAAGAA TTTTACTACC TATTGGAAGC AGCAGAAGGC 
CATGCCAAAG AAGGACAGGG TATTAAAACC GACATTCCCA GGTACATCAT TAGCCAACTG 
GGACTCAATA AGGATCCCTT GGAAGAAATG GCTCATTTGG GAAACTACGA TAGTGGGACA 
GCAGAAACAC CAGAAACAGA TGAATCAGTG AGTAGCTCTA ATGCCTCCCT GAAACTTCGA 
AGGAAACCTC GGGAAAGTGA TTTTGAAACG ATTAAATTGA TTAGCAATGG AGCCTATGGG 
GCAGTCTACT TTGTTCGGCA TAAAGAATCC CGGCAGAGGT TTGCCATGAA GAAGATTAAT 
AAACAGAACC TCATCCTTCG AAACCAGATC CAGCAGGCCT TTGTGGAGCG GGATATCCTG 
ACTTTTGCAG AAAACCCCTT TGTTGTCAGC ATGTATTGCT CCTTTGAAAC AAGGCGCCAC 
TTGTGCATGG TCATGGAATA TGTGGAAGGG GGAGACTGTG CTACTTTAAT GAAAAACATG 
GGTCCTCTCC CTGTTGATAT GGCCAGAATG TACTTTGCTG AGACGGTCTT GGCCTTGGAA 
TATTTACATA ATTATGGAAT TGTACACAGG GATTTGAAAC CAGACAACTT GTTGGTTACC 
TCCATGGGGC ACATAAAGCT GACAGATTTT GGATTATCTA AGGTGGGACT AATGAGCATG 
ACTACCAACC TTTACGAGGG TCATATTGAG AAGGATGCTA GAGAGTTCCT GGATAAACAG 
GTCTGTGGCA CACCTGAATA CATTGCACCA GAAGTGATTC TGAGGCAGGG TTATGGAAAG 
CCGGTGGACT GGTGGGCCAT GGGGATTATC CTCTATGAAT TTCTGGTTGG ATGCGTGCCA 
TTCTTTGGGG ATACTCCAGA GGAGCTATTT GGACAAGTCA TCAGTGATGA GATCAACTGG 
CCTGAGAAGG ATGAGGCACC CCCACCTGAT GCCCAGGATC TGATTACCTT ACTCCTCAGG 
CAGAATCCCC TGGAGAGGCT GGGAACAGGT GGTGCATATG AAGTCAAACA GCATCGATTC 
TTCCGTTCTT TAGACTGGAA CAGTTTGCTG AGACAGAAGG CAGAATTTAT TCCCCAACTG 
GAATCTGAGG ATGACACAAG TTATTTTGAT ACTCGGTCTG AGAAGTATCA TCATATGGAA 
ACGGAGGAAG AAGATGACAC AAATGATGAA GACTTTAATG TGGAAATAAG GCAGTTTTCT 
TCATGTTCAC ACAGGTTTTC AAAAGTTTTC AGCAGTATAG ATCGAATCAC TCAGAATTCA 
GCAGAAGAGA AGGAAGACTC TGTGGACAAA ACCAAAAGCA CCACCTTGCC ATCCACAGAA 
ACACTGAGCT GGAGTTCAGA ATATTCTGAA ATGCAACAGC TATCAACATC CAACTCTTCA 
GATACTGAAA GCAACAGACA TAAACTCAGT TCTGGCCTAC TTCCCAAACT GGCTATTTCA 
ACAGAGGGAG AGCAAGATGA AGCTGCCTCC TGCCCTGGAG ACCCCCATGA GGAGCCAGGA 
AAGCCAGCCC TTCCTCCTGA AGAGTGTGCC CAGGAGGAGC CTGAGGTCAC CACCCCAGCC 
AGCACCATCA GCAGCTCCAC CCTGTCAGTT GGCAGTTTTT CAGAGCACTT GGATCAGATA 
AATGGACGAA GCGAGTGTGT GGACAGTACA GATAATTCCT CAAAGCCATC CAGTGAACCC 
GCTTCTCACA TGGCTCGGCA GCGATTAGAA AGCACAGAAA AAAAGAAAAT CTCGGGGAAA 
GTCACAAAGT CCCTCTCTGC CAGTGCTCTT TCCCTCATGA TCCCAGGAGA TATGTTTGCT 
GTTTCCCCTC TGGGAAGTCC AATGTCTCCC CATTCCCTGT CCTCGGACCC TTCTTCTTCA 
CGAGATTCCT CTCCCAGCCG AGATTCCTCA GCAGCTTCTG CCAGTCCACA TCAGCCGATT 
GTGATCCACA GTTCGGGGAA GAACTACGGC TTTACCATCC GAGCCATCCG GGTGTATGTG 
GGAGACAGTG ACATCTATAC AGTGCACCAT ATCGTCTGGA ATGTAGAAGA AGGAAGTCCG 
GCATGCCAGG CAGGACTGAA GGCTGGAGAT CTTATCACTC ACATCAATGG AGAACCAGTG 
CATGGACTTG TCCACACAGA AGTTATAGAA CTCCTACTGA AGAGTGGGAA TAAGGTGTCA 
ATCACTACTA CCCCATTTGA AAACACATCA ATCAAAACTG GACCAGCCAG GAGAAACAGC 
TATAAGAGCC GGATGGTGAG GCGGAGCAAG AAATCCAAGA AGAAAGAAAG TCTCGAAAGG 
AGGAGATCTC TTTTCAAAAA GCTAGCCAAG CAGCCTTCTC CTTTACTCCA CACCAGCCGA 
AGTTTCTCCT GCTTGAACAG ATCCCTGTCA TCGGGTGAGA GCCTCCCAGG TTCCCCCACT 
CATAGCTTGT CTCCCCGGTC TCCAACACCA AGCTACCGCT CCACCCCTGA CTTCCCATCT 
GGTACTAATT CCTCCCAGAG CAGCTCCCCT AGTTCTAGTG CCCCCAATTC CCCAGCAGGG 
TCCGGGCACA TCCGGCCCAG CACTCTCCAC GGTCTTGCAC CCAAACTCGG CGGGCAGCGG 
TACCGGTCCG GAAGGCGAAA GTCCGCCGGC AACATCCCAC TGTCCCCGCT GGCCCGGACG 
CCCTCTCCAA CCCCGCAACC CACCTCCCCG CAGCGGTCAC CATCCCCTCT TCTGGGACAC 
TCACTGGGCA ATTCCAAGAT CGCGCAAGCC TTTCCCAGCA AGATGCACTC CCCGCCCACC 
ATCGTCAGAC ACATCGTGAG GCCCAAGAGT GCGGAGCCCC CCAGGTCCCC GCTGCTCAAG 
CGCGTGCAGT CCGAGGAGAA GCTGTCGCCC TCTTACGGCA GTGACAAGAA GCACCTGTGC 
TCCCGCAAGC ACAGCCTGGA GGTGACCCAA GAGGAGGTGC AGCGGGAGCA GTCCCAGCGG 
GAGGCGCCGC TGCAGAGCCT GGATGAGAAC GTGTGCGACG TGCCGCCGCT CAGCCGCGCC 
CGGCCAGTGG AGCAAGGCTG CCTGAAACGC CCAGTCTCCC GGAAGGTGGG CCGCCAGGAG 
TCTGTGGACG ACCTGGACCG CGACAAGCTG AAGGCCAAGG TGGTGGTGAA GAAAGCAGAC 
GGCTTCCCAG AGAAACAGGA ATCCCACCAG AAATCCCATG GACCCGGGAG TGATTTGGAA 
AACTTTGCTC TGTTTAAGCT GGAAGAGAGA GAGAAGAAAG TCTATCCGAA GGCTGTGGAA 
AGGTCAAGTA CTTTTGAAAA CAAAGCGTCT ATGCAGGAGG CGCCACCGCT GGGCAGCCTG 
CTGAAGGATG CTCTTCACAA GCAGGCCAGC GTGCGCGCCA GCGAGGGTGC GATGTCGGAT 
GGCCGGGTGC CTGCGGAGCA CCGCCAGGGT GGCGGGGACT TCAGACGGGC CCCCGCTCCT 
GGCACCCTCC AGGATGGTCT CTGCCACTCC CTCGACAGGG GCATCTCTGG GAAGGGGGAA 
GGCACGGAGA AGTCCTCCCA GGCCAAGGAG CTTCTCCGAT GTGAAAAGTT AGACAGCAAG 
CTGGCCAACA TCGATTACCT CCGAAAGAAA ATGTCACTTG AGGACAAAGA GGACAACCTC 
TGCCCTGTGC TGAAGCCCAA GATGACAGCT GGCTCCCACG AATGCCTGCC AGGGAACCCA 
GTCCGACCCA CGGGTGGGCA GCAGGAGCCC CCGCCGGCTT CTGAGAGCCG AGCTTTTGTC 
AGCAGCACCC ATGCAGCTCA GATGAGTGCC GTCTCTTTTG TTCCCCTCAA GGCCTTAACA 
GGCCGGGTGG ACAGTGGAAC GGAGAAGCCT GGCTTGGTTG CTCCTGAGTC CCCTGTTAGG 
AAGAGCCCCT CCGAGTATAA GCTGGAAGGT AGGTCTGTCT CATGCCTGAA GCCGATCGAG 
GGCACTCTGG ACATTGCTCT CCTGTCCGGA CCTCAGGCCT CCAAGACAGA ACTGCCTTCC 
CCAGAGTCTG CACAGAGCCC CAGCCCAAGT GGTGACGTGA GGGCCTCTGT GCCACCAGTT 
CTCCCCAGCA GCAGTGGGAA AAAGAACGAT ACCACCAGTG CAAGAGAGCT TTCTCCTTCC 
AGCTTAAAGA TGAATAAATC CTACCTGCTG GAGCCTTGGT TCCTGCCCCC CAGCCGAGGT 
CTCCAGAATT CACCAGCAGT TTCCCTGCCT GACCCAGAGT TCAAGAGGGA CAGGAAAGGT 
CCCCATCCTA CTGCCAGGAG CCCTGGAACA GTCATGGAAA GCAATCCCCA ACAGAGAGAG 
GGCAGCTCCC CTAAACACCA AGACCACACC ACTGACCCCA AGCTTCTGAC CTGCCTGGGG 
CAGAACCTCC ACAGCCCTGA CCTGGCCAGG CCACGCTGCC CGCTCCCACC TGAAGCTTCC 
CCCTCAAGGG AGAAGCCAGG CCTGAGGGAA TCGTCTGAAA GAGGCCCTCC CACAGCCAGA 
AGCGAGCGCT CTGCTGCGAG GGCTGACACA TGCAGAGAGC CCTCCATGGA ACTGTGCTTT 
CCAGAAACTG CGAAAACCAG TGACAACTCC AAAAATCTCC TCTCTGTGGG AAGGACCCAC 
CCAGATTTCT ATACACAGAC CCAGGCCATG GAGAAAGCAT GGGCGCCGGG TGGGAAAACG 
AACCACAAAG ATGGCCCAGG TGAGGCGAGG CCCCCGCCCA GAGACAACTC CTCTCTGCAC 
TCAGCTGGAA TTCCCTGTGA GAAGGAGCTG GGCAAGGTGA GGCGTGGCGT GGAACCCAAG 
CCCGAAGCGC TTCTTGCCAG GCGGTCTCTG CAGCCACCTG GAATTGAGAG TGAGAAGAGT 
GAAAAGCTCT CCAGTTTCCC ATCTTTGCAG AAAGATGGTG CCAAGGAACC TGAAAGGAAG 
GAGCAGCCTC TACAAAGGCA TCCCAGCAGC ATCCCTCCGC CCCCTCTGAC GGCCAAAGAC 
CTGTCCAGCC CGGCTGCCAG GCAGCATTGC AGTTCCCCAA GCCACGCTTC TGGCAGAGAG 
CCGGGGGCCA AGCCCAGCAC TGCAGAGCCC AGCTCGAGCC CCCAGGACCC TCCCAAGCCT 
GTTGCTGCGC ACAGTGAAAG CAGCAGCCAC AAGCCCCGGC CTGGCCCTGA CCCGGGCCCT 
CCAAAGACTA AGCACCCCGA CCGGTCCCTC TCCTCTCAGA AACCAAGTGT CGGGGCCACA 
AAGGGCAAAG AGCCTGCCAC TCAGTCCCTC GGTGGCTCTA GCAGAGAGGG GAAGGGCCAC 
AGTAAGAGTG GGCCGGATGT GTTTCCTGCT ACCCCAGGCT CCCAGAACAA AGCCAGCGAT 
GGGATTGGCC AGGGAGAAGG TGGGCCCTCT GTCCCACTGC ACACTGACAG GGCTCCTCTA 
GACGCCAAGC CACAACCCAC CAGTGGTGGG CGGCCCCTGG AGGTGCTGGA GAAGCCTGTG 
CATTTGCCAA GGCCGGGACA CCCAGGGCCT AGTGAGCCAG CGGACCAGAA ACTGTCCGCT 
GTTGGTGAAA AGCAAACCCT GTCTCCAAAG CACCCCAAAC CATCCACTGT GAAAGATTGC 
CCCACCCTGT GCAAACAGAC AGACAACAGA CAGACAGACA AAAGCCCGAG TCAGCCGGCC 
GCCAACACCG ACAGAAGGGC GGAAGGGAAG AAATGCACTG AAGCACTTTA TGCTCCAGCA 
GAGGGCGACA AGCTCGAGGC CGGCCTTTCC TTTGTGCATA GCGAGAACCG GTTGAAAGGC 
GCGGAGCGGC CAGCCGCGGG GGTGGGGAAG GGCTTCCCTG AGGCCAGAGG GAAAGGGCCC 
GGTCCCCAGA AGCCACCGAC GGAGGCAGAC AAGCCCAATG GCATGAAACG GTCCCCCTCA 
GCCACTGGGC AGAGTTCTTT CCGATCCACG GCCCTCCCGG AAAAGTCTCT GAGCTGCTCC 
TCCAGCTTCC CTGAAACCAG GGCCGGAGTT AGAGAGGCCT CTGCAGCCAG CAGCGACACC 
TCTTCTGCCA AGGCCGCCGG GGGCATGCTG GAGCTTCCAG CCCCCAGCAA CAGGGACCAT 
AGGAAGGCTC AGCCTGCCGG GGAGGGCCGA ACCCACATGA CAAAGAGTGA CTCCCTGCCC 
TCCTTCCGGG TCTCCACCCT GCCTCTGGAG TCACACCACC CCGACCCAAA CACCATGGGC 
GGGGCCAGCC ACCGGGACAG GGCTCTCTCG GTGACTGCCA CCGTAGGGGA AACCAAAGGG 
AAGGACCCTG CCCCAGCCCA GCCTCCCCCA GCTAGGAAAC AGAACGTGGG CAGAGACGTG 
ACCAAGCCAT CCCCAGCCCC AAACACTGAC CGCCCCATCT CTCTTTCTAA TGAGAAGGAC 
TTTGTGGTAC GGCAGAGGCG GGGGAAAGAG AGTTTGCGTA GCAGCCCTCA CAAAAAGGCC 
TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_017009450.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864939.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X5, mRNA.

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CloneID	OHu95871
Clone ID Related Accession (Same CDS sequence)	XM_017009450.1
Accession Version	XM_017009450.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TTTGCCGAAC AAGCAACCGG AAAAGCTTAA TAGGCAATGG GCAGTCACCA 
GCATTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGATAGT 
CCAAGAAATT TCTCCCCCAG TGCCTCAGCC CATTTTTCAT TTGCACGGAG GACTGATGGA 
CGCCGCTGGT CGTTGGCTTC TCTCCCTTCC TCTGGCTATG GGACAAACAC ACCCAGCTCT 
ACGGTCTCTT CATCCTGTTC CTCCCAGGAG AAGTTGCATC AGTTACCATA CCAACCAACA 
CCAGACGAGT TACACTTCTT ATCAAAACAT TTCTGTACCA CCGAAAGCAT CGCCACTGAG 
AACAGATGCA GGAACACGCC GATGCGCCCC CGTTCCCGAA GTCTGAGCCC TGGACGTTCT 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCTACAG CTCAGATGGA AGAACGTCTA AAGGAAATTA TCACCAGCTA CTCTCCTGAC 
AACGTTCTAC CCTTAGCAGA TGGAGTGCTT AGTTTCACTC ACCACCAGAT TATTGAACTG 
GCTCGAGATT GCTTGGATAA ATCCCACCAG GGCCTCATCA CCTCACGATA CTTCCTTGAA 
TTACAGCACA AATTAGATAA GTTGCTACAG GAGGCTCATG ATCGTTCAGA AAGTGGAGAA 
TTGGCATTTA TTAAACAACT AGTTCGAAAG ATCCTAATTG TTATTGCCCG CCCTGCTCGG 
TTATTAGAGT GCCTGGAATT TGATCCGGAA GAATTTTACT ACCTATTGGA AGCAGCAGAA 
GGCCATGCCA AAGAAGGACA GGGTATTAAA ACCGACATTC CCAGGTACAT CATTAGCCAA 
CTGGGACTCA ATAAGGATCC CTTGGAAGAA ATGGCTCATT TGGGAAACTA CGATAGTGGG 
ACAGCAGAAA CACCAGAAAC AGATGAATCA GTGAGTAGCT CTAATGCCTC CCTGAAACTT 
CGAAGGAAAC CTCGGGAAAG TGATTTTGAA ACGATTAAAT TGATTAGCAA TGGAGCCTAT 
GGGGCAGTCT ACTTTGTTCG GCATAAAGAA TCCCGGCAGA GGTTTGCCAT GAAGAAGATT 
AATAAACAGA ACCTCATCCT TCGAAACCAG ATCCAGCAGG CCTTTGTGGA GCGGGATATC 
CTGACTTTTG CAGAAAACCC CTTTGTTGTC AGCATGTATT GCTCCTTTGA AACAAGGCGC 
CACTTGTGCA TGGTCATGGA ATATGTGGAA GGGGGAGACT GTGCTACTTT AATGAAAAAC 
ATGGGTCCTC TCCCTGTTGA TATGGCCAGA ATGTACTTTG CTGAGACGGT CTTGGCCTTG 
GAATATTTAC ATAATTATGG AATTGTACAC AGGGATTTGA AACCAGACAA CTTGTTGGTT 
ACCTCCATGG GGCACATAAA GCTGACAGAT TTTGGATTAT CTAAGGTGGG ACTAATGAGC 
ATGACTACCA ACCTTTACGA GGGTCATATT GAGAAGGATG CTAGAGAGTT CCTGGATAAA 
CAGGTCTGTG GCACACCTGA ATACATTGCA CCAGAAGTGA TTCTGAGGCA GGGTTATGGA 
AAGCCGGTGG ACTGGTGGGC CATGGGGATT ATCCTCTATG AATTTCTGGT TGGATGCGTG 
CCATTCTTTG GGGATACTCC AGAGGAGCTA TTTGGACAAG TCATCAGTGA TGAGATCAAC 
TGGCCTGAGA AGGATGAGGC ACCCCCACCT GATGCCCAGG ATCTGATTAC CTTACTCCTC 
AGGCAGAATC CCCTGGAGAG GCTGGGAACA GGTGGTGCAT ATGAAGTCAA ACAGCATCGA 
TTCTTCCGTT CTTTAGACTG GAACAGTTTG CTGAGACAGA AGGCAGAATT TATTCCCCAA 
CTGGAATCTG AGGATGACAC AAGTTATTTT GATACTCGGT CTGAGAAGTA TCATCATATG 
GAAACGGAGG AAGAAGATGA CACAAATGAT GAAGACTTTA ATGTGGAAAT AAGGCAGTTT 
TCTTCATGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGAAT CACTCAGAAT 
TCAGCAGAAG AGAAGGAAGA CTCTGTGGAC AAAACCAAAA GCACCACCTT GCCATCCACA 
GAAACACTGA GCTGGAGTTC AGAATATTCT GAAATGCAAC AGCTATCAAC ATCCAACTCT 
TCAGATACTG AAAGCAACAG ACATAAACTC AGTTCTGGCC TACTTCCCAA ACTGGCTATT 
TCAACAGAGG GAGAGCAAGA TGAAGCTGCC TCCTGCCCTG GAGACCCCCA TGAGGAGCCA 
GGAAAGCCAG CCCTTCCTCC TGAAGAGTGT GCCCAGGAGG AGCCTGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGCTC CACCCTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGAC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCGCTTCTC ACATGGCTCG GCAGCGATTA GAAAGCACAG AAAAAAAGAA AATCTCGGGG 
AAAGTCACAA AGTCCCTCTC TGCCAGTGCT CTTTCCCTCA TGATCCCAGG AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864939.1
CDS	97..7365
Translation	MDESSILRRRGLQKELSLPRRGSFCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_017009450.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009450.1

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TTTGCCGAAC AAGCAACCGG AAAAGCTTAA TAGGCAATGG GCAGTCACCA 
GCATTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGATAGT 
CCAAGAAATT TCTCCCCCAG TGCCTCAGCC CATTTTTCAT TTGCACGGAG GACTGATGGA 
CGCCGCTGGT CGTTGGCTTC TCTCCCTTCC TCTGGCTATG GGACAAACAC ACCCAGCTCT 
ACGGTCTCTT CATCCTGTTC CTCCCAGGAG AAGTTGCATC AGTTACCATA CCAACCAACA 
CCAGACGAGT TACACTTCTT ATCAAAACAT TTCTGTACCA CCGAAAGCAT CGCCACTGAG 
AACAGATGCA GGAACACGCC GATGCGCCCC CGTTCCCGAA GTCTGAGCCC TGGACGTTCT 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCTACAG CTCAGATGGA AGAACGTCTA AAGGAAATTA TCACCAGCTA CTCTCCTGAC 
AACGTTCTAC CCTTAGCAGA TGGAGTGCTT AGTTTCACTC ACCACCAGAT TATTGAACTG 
GCTCGAGATT GCTTGGATAA ATCCCACCAG GGCCTCATCA CCTCACGATA CTTCCTTGAA 
TTACAGCACA AATTAGATAA GTTGCTACAG GAGGCTCATG ATCGTTCAGA AAGTGGAGAA 
TTGGCATTTA TTAAACAACT AGTTCGAAAG ATCCTAATTG TTATTGCCCG CCCTGCTCGG 
TTATTAGAGT GCCTGGAATT TGATCCGGAA GAATTTTACT ACCTATTGGA AGCAGCAGAA 
GGCCATGCCA AAGAAGGACA GGGTATTAAA ACCGACATTC CCAGGTACAT CATTAGCCAA 
CTGGGACTCA ATAAGGATCC CTTGGAAGAA ATGGCTCATT TGGGAAACTA CGATAGTGGG 
ACAGCAGAAA CACCAGAAAC AGATGAATCA GTGAGTAGCT CTAATGCCTC CCTGAAACTT 
CGAAGGAAAC CTCGGGAAAG TGATTTTGAA ACGATTAAAT TGATTAGCAA TGGAGCCTAT 
GGGGCAGTCT ACTTTGTTCG GCATAAAGAA TCCCGGCAGA GGTTTGCCAT GAAGAAGATT 
AATAAACAGA ACCTCATCCT TCGAAACCAG ATCCAGCAGG CCTTTGTGGA GCGGGATATC 
CTGACTTTTG CAGAAAACCC CTTTGTTGTC AGCATGTATT GCTCCTTTGA AACAAGGCGC 
CACTTGTGCA TGGTCATGGA ATATGTGGAA GGGGGAGACT GTGCTACTTT AATGAAAAAC 
ATGGGTCCTC TCCCTGTTGA TATGGCCAGA ATGTACTTTG CTGAGACGGT CTTGGCCTTG 
GAATATTTAC ATAATTATGG AATTGTACAC AGGGATTTGA AACCAGACAA CTTGTTGGTT 
ACCTCCATGG GGCACATAAA GCTGACAGAT TTTGGATTAT CTAAGGTGGG ACTAATGAGC 
ATGACTACCA ACCTTTACGA GGGTCATATT GAGAAGGATG CTAGAGAGTT CCTGGATAAA 
CAGGTCTGTG GCACACCTGA ATACATTGCA CCAGAAGTGA TTCTGAGGCA GGGTTATGGA 
AAGCCGGTGG ACTGGTGGGC CATGGGGATT ATCCTCTATG AATTTCTGGT TGGATGCGTG 
CCATTCTTTG GGGATACTCC AGAGGAGCTA TTTGGACAAG TCATCAGTGA TGAGATCAAC 
TGGCCTGAGA AGGATGAGGC ACCCCCACCT GATGCCCAGG ATCTGATTAC CTTACTCCTC 
AGGCAGAATC CCCTGGAGAG GCTGGGAACA GGTGGTGCAT ATGAAGTCAA ACAGCATCGA 
TTCTTCCGTT CTTTAGACTG GAACAGTTTG CTGAGACAGA AGGCAGAATT TATTCCCCAA 
CTGGAATCTG AGGATGACAC AAGTTATTTT GATACTCGGT CTGAGAAGTA TCATCATATG 
GAAACGGAGG AAGAAGATGA CACAAATGAT GAAGACTTTA ATGTGGAAAT AAGGCAGTTT 
TCTTCATGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGAAT CACTCAGAAT 
TCAGCAGAAG AGAAGGAAGA CTCTGTGGAC AAAACCAAAA GCACCACCTT GCCATCCACA 
GAAACACTGA GCTGGAGTTC AGAATATTCT GAAATGCAAC AGCTATCAAC ATCCAACTCT 
TCAGATACTG AAAGCAACAG ACATAAACTC AGTTCTGGCC TACTTCCCAA ACTGGCTATT 
TCAACAGAGG GAGAGCAAGA TGAAGCTGCC TCCTGCCCTG GAGACCCCCA TGAGGAGCCA 
GGAAAGCCAG CCCTTCCTCC TGAAGAGTGT GCCCAGGAGG AGCCTGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGCTC CACCCTGTCA GTTGGCAGTT TTTCAGAGCA CTTGGATCAG 
ATAAATGGAC GAAGCGAGTG TGTGGACAGT ACAGATAATT CCTCAAAGCC ATCCAGTGAA 
CCCGCTTCTC ACATGGCTCG GCAGCGATTA GAAAGCACAG AAAAAAAGAA AATCTCGGGG 
AAAGTCACAA AGTCCCTCTC TGCCAGTGCT CTTTCCCTCA TGATCCCAGG AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_017009451.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864940.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X9, mRNA.

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CloneID	OHu95872
Clone ID Related Accession (Same CDS sequence)	XM_017009451.1
Accession Version	XM_017009451.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TTTGCCGAAC AAGCAACCGG AAAAGCTTAA TAGGCAATGG GCAGTCACCA 
GCATTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGATAGT 
CCAAGAAATT TCTCCCCCAG TGCCTCAGCC CATTTTTCAT TTGCACGGAG GACTGATGGA 
CGCCGCTGGT CGTTGGCTTC TCTCCCTTCC TCTGGCTATG GGACAAACAC ACCCAGCTCT 
ACGGTCTCTT CATCCTGTTC CTCCCAGGAG AAGTTGCATC AGTTACCATA CCAACCAACA 
CCAGACGAGT TACACTTCTT ATCAAAACAT TTCTGTACCA CCGAAAGCAT CGCCACTGAG 
AACAGATGCA GGAACACGCC GATGCGCCCC CGTTCCCGAA GTCTGAGCCC TGGACGTTCT 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCTACAG CTCAGATGGA AGAACGTCTA AAGGAAATTA TCACCAGCTA CTCTCCTGAC 
AACGTTCTAC CCTTAGCAGA TGGAGTGCTT AGTTTCACTC ACCACCAGAT TATTGAACTG 
GCTCGAGATT GCTTGGATAA ATCCCACCAG GGCCTCATCA CCTCACGATA CTTCCTTGAA 
TTACAGCACA AATTAGATAA GTTGCTACAG GAGGCTCATG ATCGTTCAGA AAGTGGAGAA 
TTGGCATTTA TTAAACAACT AGTTCGAAAG ATCCTAATTG TTATTGCCCG CCCTGCTCGG 
TTATTAGAGT GCCTGGAATT TGATCCGGAA GAATTTTACT ACCTATTGGA AGCAGCAGAA 
GGCCATGCCA AAGAAGGACA GGGTATTAAA ACCGACATTC CCAGGTACAT CATTAGCCAA 
CTGGGACTCA ATAAGGATCC CTTGGAAGAA ATGGCTCATT TGGGAAACTA CGATAGTGGG 
ACAGCAGAAA CACCAGAAAC AGATGAATCA GTGAGTAGCT CTAATGCCTC CCTGAAACTT 
CGAAGGAAAC CTCGGGAAAG TGATTTTGAA ACGATTAAAT TGATTAGCAA TGGAGCCTAT 
GGGGCAGTCT ACTTTGTTCG GCATAAAGAA TCCCGGCAGA GGTTTGCCAT GAAGAAGATT 
AATAAACAGA ACCTCATCCT TCGAAACCAG ATCCAGCAGG CCTTTGTGGA GCGGGATATC 
CTGACTTTTG CAGAAAACCC CTTTGTTGTC AGCATGTATT GCTCCTTTGA AACAAGGCGC 
CACTTGTGCA TGGTCATGGA ATATGTGGAA GGGGGAGACT GTGCTACTTT AATGAAAAAC 
ATGGGTCCTC TCCCTGTTGA TATGGCCAGA ATGTACTTTG CTGAGACGGT CTTGGCCTTG 
GAATATTTAC ATAATTATGG AATTGTACAC AGGGATTTGA AACCAGACAA CTTGTTGGTT 
ACCTCCATGG GGCACATAAA GCTGACAGAT TTTGGATTAT CTAAGGTGGG ACTAATGAGC 
ATGACTACCA ACCTTTACGA GGGTCATATT GAGAAGGATG CTAGAGAGTT CCTGGATAAA 
CAGGTCTGTG GCACACCTGA ATACATTGCA CCAGAAGTGA TTCTGAGGCA GGGTTATGGA 
AAGCCGGTGG ACTGGTGGGC CATGGGGATT ATCCTCTATG AATTTCTGGT TGGATGCGTG 
CCATTCTTTG GGGATACTCC AGAGGAGCTA TTTGGACAAG TCATCAGTGA TGAGATCAAC 
TGGCCTGAGA AGGATGAGGC ACCCCCACCT GATGCCCAGG ATCTGATTAC CTTACTCCTC 
AGGCAGAATC CCCTGGAGAG GCTGGGAACA GGTGGTGCAT ATGAAGTCAA ACAGCATCGA 
TTCTTCCGTT CTTTAGACTG GAACAGTTTG CTGAGACAGA AGGCAGAATT TATTCCCCAA 
CTGGAATCTG AGGATGACAC AAGTTATTTT GATACTCGGT CTGAGAAGTA TCATCATATG 
GAAACGGAGG AAGAAGATGA CACAAATGAT GAAGACTTTA ATGTGGAAAT AAGGCAGTTT 
TCTTCATGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGAAT CACTCAGAAT 
TCAGCAGAAG AGAAGGAAGA CTCTGTGGAC AAAACCAAAA GCACCACCTT GCCATCCACA 
GAAACACTGA GCTGGAGTTC AGAATATTCT GAAATGCAAC AGCTATCAAC ATCCAACTCT 
TCAGATACTG AAAGCAACAG ACATAAACTC AGTTCTGGCC TACTTCCCAA ACTGGCTATT 
TCAACAGAGG GAGAGCAAGA TGAAGCTGCC TCCTGCCCTG GAGACCCCCA TGAGGAGCCA 
GGAAAGCCAG CCCTTCCTCC TGAAGAGTGT GCCCAGGAGG AGCCTGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGCTC CACCCTGTCA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864940.1
CDS	97..7164
Translation	MDESSILRRRGLQKELSLPRRGSFCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_017009451.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009451.1

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TTTGCCGAAC AAGCAACCGG AAAAGCTTAA TAGGCAATGG GCAGTCACCA 
GCATTGCCTC GACCACACTC ACCTCTCTCT GCTCATGCAG GAAATAGCCC TCAAGATAGT 
CCAAGAAATT TCTCCCCCAG TGCCTCAGCC CATTTTTCAT TTGCACGGAG GACTGATGGA 
CGCCGCTGGT CGTTGGCTTC TCTCCCTTCC TCTGGCTATG GGACAAACAC ACCCAGCTCT 
ACGGTCTCTT CATCCTGTTC CTCCCAGGAG AAGTTGCATC AGTTACCATA CCAACCAACA 
CCAGACGAGT TACACTTCTT ATCAAAACAT TTCTGTACCA CCGAAAGCAT CGCCACTGAG 
AACAGATGCA GGAACACGCC GATGCGCCCC CGTTCCCGAA GTCTGAGCCC TGGACGTTCT 
CCCGCCTGCT GTGACCATGA AATAATTATG ATGAACCATG TCTACAAAGA AAGGTTCCCA 
AAGGCTACAG CTCAGATGGA AGAACGTCTA AAGGAAATTA TCACCAGCTA CTCTCCTGAC 
AACGTTCTAC CCTTAGCAGA TGGAGTGCTT AGTTTCACTC ACCACCAGAT TATTGAACTG 
GCTCGAGATT GCTTGGATAA ATCCCACCAG GGCCTCATCA CCTCACGATA CTTCCTTGAA 
TTACAGCACA AATTAGATAA GTTGCTACAG GAGGCTCATG ATCGTTCAGA AAGTGGAGAA 
TTGGCATTTA TTAAACAACT AGTTCGAAAG ATCCTAATTG TTATTGCCCG CCCTGCTCGG 
TTATTAGAGT GCCTGGAATT TGATCCGGAA GAATTTTACT ACCTATTGGA AGCAGCAGAA 
GGCCATGCCA AAGAAGGACA GGGTATTAAA ACCGACATTC CCAGGTACAT CATTAGCCAA 
CTGGGACTCA ATAAGGATCC CTTGGAAGAA ATGGCTCATT TGGGAAACTA CGATAGTGGG 
ACAGCAGAAA CACCAGAAAC AGATGAATCA GTGAGTAGCT CTAATGCCTC CCTGAAACTT 
CGAAGGAAAC CTCGGGAAAG TGATTTTGAA ACGATTAAAT TGATTAGCAA TGGAGCCTAT 
GGGGCAGTCT ACTTTGTTCG GCATAAAGAA TCCCGGCAGA GGTTTGCCAT GAAGAAGATT 
AATAAACAGA ACCTCATCCT TCGAAACCAG ATCCAGCAGG CCTTTGTGGA GCGGGATATC 
CTGACTTTTG CAGAAAACCC CTTTGTTGTC AGCATGTATT GCTCCTTTGA AACAAGGCGC 
CACTTGTGCA TGGTCATGGA ATATGTGGAA GGGGGAGACT GTGCTACTTT AATGAAAAAC 
ATGGGTCCTC TCCCTGTTGA TATGGCCAGA ATGTACTTTG CTGAGACGGT CTTGGCCTTG 
GAATATTTAC ATAATTATGG AATTGTACAC AGGGATTTGA AACCAGACAA CTTGTTGGTT 
ACCTCCATGG GGCACATAAA GCTGACAGAT TTTGGATTAT CTAAGGTGGG ACTAATGAGC 
ATGACTACCA ACCTTTACGA GGGTCATATT GAGAAGGATG CTAGAGAGTT CCTGGATAAA 
CAGGTCTGTG GCACACCTGA ATACATTGCA CCAGAAGTGA TTCTGAGGCA GGGTTATGGA 
AAGCCGGTGG ACTGGTGGGC CATGGGGATT ATCCTCTATG AATTTCTGGT TGGATGCGTG 
CCATTCTTTG GGGATACTCC AGAGGAGCTA TTTGGACAAG TCATCAGTGA TGAGATCAAC 
TGGCCTGAGA AGGATGAGGC ACCCCCACCT GATGCCCAGG ATCTGATTAC CTTACTCCTC 
AGGCAGAATC CCCTGGAGAG GCTGGGAACA GGTGGTGCAT ATGAAGTCAA ACAGCATCGA 
TTCTTCCGTT CTTTAGACTG GAACAGTTTG CTGAGACAGA AGGCAGAATT TATTCCCCAA 
CTGGAATCTG AGGATGACAC AAGTTATTTT GATACTCGGT CTGAGAAGTA TCATCATATG 
GAAACGGAGG AAGAAGATGA CACAAATGAT GAAGACTTTA ATGTGGAAAT AAGGCAGTTT 
TCTTCATGTT CACACAGGTT TTCAAAAGTT TTCAGCAGTA TAGATCGAAT CACTCAGAAT 
TCAGCAGAAG AGAAGGAAGA CTCTGTGGAC AAAACCAAAA GCACCACCTT GCCATCCACA 
GAAACACTGA GCTGGAGTTC AGAATATTCT GAAATGCAAC AGCTATCAAC ATCCAACTCT 
TCAGATACTG AAAGCAACAG ACATAAACTC AGTTCTGGCC TACTTCCCAA ACTGGCTATT 
TCAACAGAGG GAGAGCAAGA TGAAGCTGCC TCCTGCCCTG GAGACCCCCA TGAGGAGCCA 
GGAAAGCCAG CCCTTCCTCC TGAAGAGTGT GCCCAGGAGG AGCCTGAGGT CACCACCCCA 
GCCAGCACCA TCAGCAGCTC CACCCTGTCA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_017009452.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864941.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X10, mRNA.

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CloneID	OHu95873
Clone ID Related Accession (Same CDS sequence)	XM_017009452.1 , XM_017009453.1
Accession Version	XM_017009452.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCCCC GTTCCCGAAG TCTGAGCCCT GGACGTTCTC CCGCCTGCTG TGACCATGAA 
ATAATTATGA TGAACCATGT CTACAAAGAA AGGTTCCCAA AGGCTACAGC TCAGATGGAA 
GAACGTCTAA AGGAAATTAT CACCAGCTAC TCTCCTGACA ACGTTCTACC CTTAGCAGAT 
GGAGTGCTTA GTTTCACTCA CCACCAGATT ATTGAACTGG CTCGAGATTG CTTGGATAAA 
TCCCACCAGG GCCTCATCAC CTCACGATAC TTCCTTGAAT TACAGCACAA ATTAGATAAG 
TTGCTACAGG AGGCTCATGA TCGTTCAGAA AGTGGAGAAT TGGCATTTAT TAAACAACTA 
GTTCGAAAGA TCCTAATTGT TATTGCCCGC CCTGCTCGGT TATTAGAGTG CCTGGAATTT 
GATCCGGAAG AATTTTACTA CCTATTGGAA GCAGCAGAAG GCCATGCCAA AGAAGGACAG 
GGTATTAAAA CCGACATTCC CAGGTACATC ATTAGCCAAC TGGGACTCAA TAAGGATCCC 
TTGGAAGAAA TGGCTCATTT GGGAAACTAC GATAGTGGGA CAGCAGAAAC ACCAGAAACA 
GATGAATCAG TGAGTAGCTC TAATGCCTCC CTGAAACTTC GAAGGAAACC TCGGGAAAGT 
GATTTTGAAA CGATTAAATT GATTAGCAAT GGAGCCTATG GGGCAGTCTA CTTTGTTCGG 
CATAAAGAAT CCCGGCAGAG GTTTGCCATG AAGAAGATTA ATAAACAGAA CCTCATCCTT 
CGAAACCAGA TCCAGCAGGC CTTTGTGGAG CGGGATATCC TGACTTTTGC AGAAAACCCC 
TTTGTTGTCA GCATGTATTG CTCCTTTGAA ACAAGGCGCC ACTTGTGCAT GGTCATGGAA 
TATGTGGAAG GGGGAGACTG TGCTACTTTA ATGAAAAACA TGGGTCCTCT CCCTGTTGAT 
ATGGCCAGAA TGTACTTTGC TGAGACGGTC TTGGCCTTGG AATATTTACA TAATTATGGA 
ATTGTACACA GGGATTTGAA ACCAGACAAC TTGTTGGTTA CCTCCATGGG GCACATAAAG 
CTGACAGATT TTGGATTATC TAAGGTGGGA CTAATGAGCA TGACTACCAA CCTTTACGAG 
GGTCATATTG AGAAGGATGC TAGAGAGTTC CTGGATAAAC AGGTCTGTGG CACACCTGAA 
TACATTGCAC CAGAAGTGAT TCTGAGGCAG GGTTATGGAA AGCCGGTGGA CTGGTGGGCC 
ATGGGGATTA TCCTCTATGA ATTTCTGGTT GGATGCGTGC CATTCTTTGG GGATACTCCA 
GAGGAGCTAT TTGGACAAGT CATCAGTGAT GAGATCAACT GGCCTGAGAA GGATGAGGCA 
CCCCCACCTG ATGCCCAGGA TCTGATTACC TTACTCCTCA GGCAGAATCC CCTGGAGAGG 
CTGGGAACAG GTGGTGCATA TGAAGTCAAA CAGCATCGAT TCTTCCGTTC TTTAGACTGG 
AACAGTTTGC TGAGACAGAA GGCAGAATTT ATTCCCCAAC TGGAATCTGA GGATGACACA 
AGTTATTTTG ATACTCGGTC TGAGAAGTAT CATCATATGG AAACGGAGGA AGAAGATGAC 
ACAAATGATG AAGACTTTAA TGTGGAAATA AGGCAGTTTT CTTCATGTTC ACACAGGTTT 
TCAAAAGTTT TCAGCAGTAT AGATCGAATC ACTCAGAATT CAGCAGAAGA GAAGGAAGAC 
TCTGTGGACA AAACCAAAAG CACCACCTTG CCATCCACAG AAACACTGAG CTGGAGTTCA 
GAATATTCTG AAATGCAACA GCTATCAACA TCCAACTCTT CAGATACTGA AAGCAACAGA 
CATAAACTCA GTTCTGGCCT ACTTCCCAAA CTGGCTATTT CAACAGAGGG AGAGCAAGAT 
GAAGCTGCCT CCTGCCCTGG AGACCCCCAT GAGGAGCCAG GAAAGCCAGC CCTTCCTCCT 
GAAGAGTGTG CCCAGGAGGA GCCTGAGGTC ACCACCCCAG CCAGCACCAT CAGCAGCTCC 
ACCCTGTCAG TTGGCAGTTT TTCAGAGCAC TTGGATCAGA TAAATGGACG AAGCGAGTGT 
GTGGACAGTA CAGATAATTC CTCAAAGCCA TCCAGTGAAC CCGCTTCTCA CATGGCTCGG 
CAGCGATTAG AAAGCACAGA AAAAAAGAAA ATCTCGGGGA AAGTCACAAA GTCCCTCTCT 
GCCAGTGCTC TTTCCCTCAT GATCCCAGGA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864941.1
CDS	3853..10680
Translation	MRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_017009452.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009452.1

ORF Insert Sequence:

ATGCGCCCCC GTTCCCGAAG TCTGAGCCCT GGACGTTCTC CCGCCTGCTG TGACCATGAA 
ATAATTATGA TGAACCATGT CTACAAAGAA AGGTTCCCAA AGGCTACAGC TCAGATGGAA 
GAACGTCTAA AGGAAATTAT CACCAGCTAC TCTCCTGACA ACGTTCTACC CTTAGCAGAT 
GGAGTGCTTA GTTTCACTCA CCACCAGATT ATTGAACTGG CTCGAGATTG CTTGGATAAA 
TCCCACCAGG GCCTCATCAC CTCACGATAC TTCCTTGAAT TACAGCACAA ATTAGATAAG 
TTGCTACAGG AGGCTCATGA TCGTTCAGAA AGTGGAGAAT TGGCATTTAT TAAACAACTA 
GTTCGAAAGA TCCTAATTGT TATTGCCCGC CCTGCTCGGT TATTAGAGTG CCTGGAATTT 
GATCCGGAAG AATTTTACTA CCTATTGGAA GCAGCAGAAG GCCATGCCAA AGAAGGACAG 
GGTATTAAAA CCGACATTCC CAGGTACATC ATTAGCCAAC TGGGACTCAA TAAGGATCCC 
TTGGAAGAAA TGGCTCATTT GGGAAACTAC GATAGTGGGA CAGCAGAAAC ACCAGAAACA 
GATGAATCAG TGAGTAGCTC TAATGCCTCC CTGAAACTTC GAAGGAAACC TCGGGAAAGT 
GATTTTGAAA CGATTAAATT GATTAGCAAT GGAGCCTATG GGGCAGTCTA CTTTGTTCGG 
CATAAAGAAT CCCGGCAGAG GTTTGCCATG AAGAAGATTA ATAAACAGAA CCTCATCCTT 
CGAAACCAGA TCCAGCAGGC CTTTGTGGAG CGGGATATCC TGACTTTTGC AGAAAACCCC 
TTTGTTGTCA GCATGTATTG CTCCTTTGAA ACAAGGCGCC ACTTGTGCAT GGTCATGGAA 
TATGTGGAAG GGGGAGACTG TGCTACTTTA ATGAAAAACA TGGGTCCTCT CCCTGTTGAT 
ATGGCCAGAA TGTACTTTGC TGAGACGGTC TTGGCCTTGG AATATTTACA TAATTATGGA 
ATTGTACACA GGGATTTGAA ACCAGACAAC TTGTTGGTTA CCTCCATGGG GCACATAAAG 
CTGACAGATT TTGGATTATC TAAGGTGGGA CTAATGAGCA TGACTACCAA CCTTTACGAG 
GGTCATATTG AGAAGGATGC TAGAGAGTTC CTGGATAAAC AGGTCTGTGG CACACCTGAA 
TACATTGCAC CAGAAGTGAT TCTGAGGCAG GGTTATGGAA AGCCGGTGGA CTGGTGGGCC 
ATGGGGATTA TCCTCTATGA ATTTCTGGTT GGATGCGTGC CATTCTTTGG GGATACTCCA 
GAGGAGCTAT TTGGACAAGT CATCAGTGAT GAGATCAACT GGCCTGAGAA GGATGAGGCA 
CCCCCACCTG ATGCCCAGGA TCTGATTACC TTACTCCTCA GGCAGAATCC CCTGGAGAGG 
CTGGGAACAG GTGGTGCATA TGAAGTCAAA CAGCATCGAT TCTTCCGTTC TTTAGACTGG 
AACAGTTTGC TGAGACAGAA GGCAGAATTT ATTCCCCAAC TGGAATCTGA GGATGACACA 
AGTTATTTTG ATACTCGGTC TGAGAAGTAT CATCATATGG AAACGGAGGA AGAAGATGAC 
ACAAATGATG AAGACTTTAA TGTGGAAATA AGGCAGTTTT CTTCATGTTC ACACAGGTTT 
TCAAAAGTTT TCAGCAGTAT AGATCGAATC ACTCAGAATT CAGCAGAAGA GAAGGAAGAC 
TCTGTGGACA AAACCAAAAG CACCACCTTG CCATCCACAG AAACACTGAG CTGGAGTTCA 
GAATATTCTG AAATGCAACA GCTATCAACA TCCAACTCTT CAGATACTGA AAGCAACAGA 
CATAAACTCA GTTCTGGCCT ACTTCCCAAA CTGGCTATTT CAACAGAGGG AGAGCAAGAT 
GAAGCTGCCT CCTGCCCTGG AGACCCCCAT GAGGAGCCAG GAAAGCCAGC CCTTCCTCCT 
GAAGAGTGTG CCCAGGAGGA GCCTGAGGTC ACCACCCCAG CCAGCACCAT CAGCAGCTCC 
ACCCTGTCAG TTGGCAGTTT TTCAGAGCAC TTGGATCAGA TAAATGGACG AAGCGAGTGT 
GTGGACAGTA CAGATAATTC CTCAAAGCCA TCCAGTGAAC CCGCTTCTCA CATGGCTCGG 
CAGCGATTAG AAAGCACAGA AAAAAAGAAA ATCTCGGGGA AAGTCACAAA GTCCCTCTCT 
GCCAGTGCTC TTTCCCTCAT GATCCCAGGA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_017009453.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864942.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X11, mRNA.

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CloneID	OHu95873
Clone ID Related Accession (Same CDS sequence)	XM_017009452.1 , XM_017009453.1
Accession Version	XM_017009453.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6828bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCCCC GTTCCCGAAG TCTGAGCCCT GGACGTTCTC CCGCCTGCTG TGACCATGAA 
ATAATTATGA TGAACCATGT CTACAAAGAA AGGTTCCCAA AGGCTACAGC TCAGATGGAA 
GAACGTCTAA AGGAAATTAT CACCAGCTAC TCTCCTGACA ACGTTCTACC CTTAGCAGAT 
GGAGTGCTTA GTTTCACTCA CCACCAGATT ATTGAACTGG CTCGAGATTG CTTGGATAAA 
TCCCACCAGG GCCTCATCAC CTCACGATAC TTCCTTGAAT TACAGCACAA ATTAGATAAG 
TTGCTACAGG AGGCTCATGA TCGTTCAGAA AGTGGAGAAT TGGCATTTAT TAAACAACTA 
GTTCGAAAGA TCCTAATTGT TATTGCCCGC CCTGCTCGGT TATTAGAGTG CCTGGAATTT 
GATCCGGAAG AATTTTACTA CCTATTGGAA GCAGCAGAAG GCCATGCCAA AGAAGGACAG 
GGTATTAAAA CCGACATTCC CAGGTACATC ATTAGCCAAC TGGGACTCAA TAAGGATCCC 
TTGGAAGAAA TGGCTCATTT GGGAAACTAC GATAGTGGGA CAGCAGAAAC ACCAGAAACA 
GATGAATCAG TGAGTAGCTC TAATGCCTCC CTGAAACTTC GAAGGAAACC TCGGGAAAGT 
GATTTTGAAA CGATTAAATT GATTAGCAAT GGAGCCTATG GGGCAGTCTA CTTTGTTCGG 
CATAAAGAAT CCCGGCAGAG GTTTGCCATG AAGAAGATTA ATAAACAGAA CCTCATCCTT 
CGAAACCAGA TCCAGCAGGC CTTTGTGGAG CGGGATATCC TGACTTTTGC AGAAAACCCC 
TTTGTTGTCA GCATGTATTG CTCCTTTGAA ACAAGGCGCC ACTTGTGCAT GGTCATGGAA 
TATGTGGAAG GGGGAGACTG TGCTACTTTA ATGAAAAACA TGGGTCCTCT CCCTGTTGAT 
ATGGCCAGAA TGTACTTTGC TGAGACGGTC TTGGCCTTGG AATATTTACA TAATTATGGA 
ATTGTACACA GGGATTTGAA ACCAGACAAC TTGTTGGTTA CCTCCATGGG GCACATAAAG 
CTGACAGATT TTGGATTATC TAAGGTGGGA CTAATGAGCA TGACTACCAA CCTTTACGAG 
GGTCATATTG AGAAGGATGC TAGAGAGTTC CTGGATAAAC AGGTCTGTGG CACACCTGAA 
TACATTGCAC CAGAAGTGAT TCTGAGGCAG GGTTATGGAA AGCCGGTGGA CTGGTGGGCC 
ATGGGGATTA TCCTCTATGA ATTTCTGGTT GGATGCGTGC CATTCTTTGG GGATACTCCA 
GAGGAGCTAT TTGGACAAGT CATCAGTGAT GAGATCAACT GGCCTGAGAA GGATGAGGCA 
CCCCCACCTG ATGCCCAGGA TCTGATTACC TTACTCCTCA GGCAGAATCC CCTGGAGAGG 
CTGGGAACAG GTGGTGCATA TGAAGTCAAA CAGCATCGAT TCTTCCGTTC TTTAGACTGG 
AACAGTTTGC TGAGACAGAA GGCAGAATTT ATTCCCCAAC TGGAATCTGA GGATGACACA 
AGTTATTTTG ATACTCGGTC TGAGAAGTAT CATCATATGG AAACGGAGGA AGAAGATGAC 
ACAAATGATG AAGACTTTAA TGTGGAAATA AGGCAGTTTT CTTCATGTTC ACACAGGTTT 
TCAAAAGTTT TCAGCAGTAT AGATCGAATC ACTCAGAATT CAGCAGAAGA GAAGGAAGAC 
TCTGTGGACA AAACCAAAAG CACCACCTTG CCATCCACAG AAACACTGAG CTGGAGTTCA 
GAATATTCTG AAATGCAACA GCTATCAACA TCCAACTCTT CAGATACTGA AAGCAACAGA 
CATAAACTCA GTTCTGGCCT ACTTCCCAAA CTGGCTATTT CAACAGAGGG AGAGCAAGAT 
GAAGCTGCCT CCTGCCCTGG AGACCCCCAT GAGGAGCCAG GAAAGCCAGC CCTTCCTCCT 
GAAGAGTGTG CCCAGGAGGA GCCTGAGGTC ACCACCCCAG CCAGCACCAT CAGCAGCTCC 
ACCCTGTCAG TTGGCAGTTT TTCAGAGCAC TTGGATCAGA TAAATGGACG AAGCGAGTGT 
GTGGACAGTA CAGATAATTC CTCAAAGCCA TCCAGTGAAC CCGCTTCTCA CATGGCTCGG 
CAGCGATTAG AAAGCACAGA AAAAAAGAAA ATCTCGGGGA AAGTCACAAA GTCCCTCTCT 
GCCAGTGCTC TTTCCCTCAT GATCCCAGGA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864942.1
CDS	10668..17495
Translation	MRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_017009453.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009453.1

ORF Insert Sequence:

ATGCGCCCCC GTTCCCGAAG TCTGAGCCCT GGACGTTCTC CCGCCTGCTG TGACCATGAA 
ATAATTATGA TGAACCATGT CTACAAAGAA AGGTTCCCAA AGGCTACAGC TCAGATGGAA 
GAACGTCTAA AGGAAATTAT CACCAGCTAC TCTCCTGACA ACGTTCTACC CTTAGCAGAT 
GGAGTGCTTA GTTTCACTCA CCACCAGATT ATTGAACTGG CTCGAGATTG CTTGGATAAA 
TCCCACCAGG GCCTCATCAC CTCACGATAC TTCCTTGAAT TACAGCACAA ATTAGATAAG 
TTGCTACAGG AGGCTCATGA TCGTTCAGAA AGTGGAGAAT TGGCATTTAT TAAACAACTA 
GTTCGAAAGA TCCTAATTGT TATTGCCCGC CCTGCTCGGT TATTAGAGTG CCTGGAATTT 
GATCCGGAAG AATTTTACTA CCTATTGGAA GCAGCAGAAG GCCATGCCAA AGAAGGACAG 
GGTATTAAAA CCGACATTCC CAGGTACATC ATTAGCCAAC TGGGACTCAA TAAGGATCCC 
TTGGAAGAAA TGGCTCATTT GGGAAACTAC GATAGTGGGA CAGCAGAAAC ACCAGAAACA 
GATGAATCAG TGAGTAGCTC TAATGCCTCC CTGAAACTTC GAAGGAAACC TCGGGAAAGT 
GATTTTGAAA CGATTAAATT GATTAGCAAT GGAGCCTATG GGGCAGTCTA CTTTGTTCGG 
CATAAAGAAT CCCGGCAGAG GTTTGCCATG AAGAAGATTA ATAAACAGAA CCTCATCCTT 
CGAAACCAGA TCCAGCAGGC CTTTGTGGAG CGGGATATCC TGACTTTTGC AGAAAACCCC 
TTTGTTGTCA GCATGTATTG CTCCTTTGAA ACAAGGCGCC ACTTGTGCAT GGTCATGGAA 
TATGTGGAAG GGGGAGACTG TGCTACTTTA ATGAAAAACA TGGGTCCTCT CCCTGTTGAT 
ATGGCCAGAA TGTACTTTGC TGAGACGGTC TTGGCCTTGG AATATTTACA TAATTATGGA 
ATTGTACACA GGGATTTGAA ACCAGACAAC TTGTTGGTTA CCTCCATGGG GCACATAAAG 
CTGACAGATT TTGGATTATC TAAGGTGGGA CTAATGAGCA TGACTACCAA CCTTTACGAG 
GGTCATATTG AGAAGGATGC TAGAGAGTTC CTGGATAAAC AGGTCTGTGG CACACCTGAA 
TACATTGCAC CAGAAGTGAT TCTGAGGCAG GGTTATGGAA AGCCGGTGGA CTGGTGGGCC 
ATGGGGATTA TCCTCTATGA ATTTCTGGTT GGATGCGTGC CATTCTTTGG GGATACTCCA 
GAGGAGCTAT TTGGACAAGT CATCAGTGAT GAGATCAACT GGCCTGAGAA GGATGAGGCA 
CCCCCACCTG ATGCCCAGGA TCTGATTACC TTACTCCTCA GGCAGAATCC CCTGGAGAGG 
CTGGGAACAG GTGGTGCATA TGAAGTCAAA CAGCATCGAT TCTTCCGTTC TTTAGACTGG 
AACAGTTTGC TGAGACAGAA GGCAGAATTT ATTCCCCAAC TGGAATCTGA GGATGACACA 
AGTTATTTTG ATACTCGGTC TGAGAAGTAT CATCATATGG AAACGGAGGA AGAAGATGAC 
ACAAATGATG AAGACTTTAA TGTGGAAATA AGGCAGTTTT CTTCATGTTC ACACAGGTTT 
TCAAAAGTTT TCAGCAGTAT AGATCGAATC ACTCAGAATT CAGCAGAAGA GAAGGAAGAC 
TCTGTGGACA AAACCAAAAG CACCACCTTG CCATCCACAG AAACACTGAG CTGGAGTTCA 
GAATATTCTG AAATGCAACA GCTATCAACA TCCAACTCTT CAGATACTGA AAGCAACAGA 
CATAAACTCA GTTCTGGCCT ACTTCCCAAA CTGGCTATTT CAACAGAGGG AGAGCAAGAT 
GAAGCTGCCT CCTGCCCTGG AGACCCCCAT GAGGAGCCAG GAAAGCCAGC CCTTCCTCCT 
GAAGAGTGTG CCCAGGAGGA GCCTGAGGTC ACCACCCCAG CCAGCACCAT CAGCAGCTCC 
ACCCTGTCAG TTGGCAGTTT TTCAGAGCAC TTGGATCAGA TAAATGGACG AAGCGAGTGT 
GTGGACAGTA CAGATAATTC CTCAAAGCCA TCCAGTGAAC CCGCTTCTCA CATGGCTCGG 
CAGCGATTAG AAAGCACAGA AAAAAAGAAA ATCTCGGGGA AAGTCACAAA GTCCCTCTCT 
GCCAGTGCTC TTTCCCTCAT GATCCCAGGA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_015183.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055998.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 1, mRNA.

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CloneID	OHu14568
Clone ID Related Accession (Same CDS sequence)	NM_015183.3 , NM_015183.2
Accession Version	NM_015183.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC016634.7, AY830839.1 and AC044799.5. On Jun 2, 2019 this sequence version replaced NM_015183.2. Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, and includes an alternate in-frame exon in the 5' coding region, compared to variant 3. The resulting isoform (a) has a shorter N-terminus and includes an additional segment near the N-terminus, compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY830839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154361, SAMEA2159607 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TGATAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055998.1
CDS	72..7376
Translation	MDESSILRRRGLQKELSLPRRGSLIDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_015183.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015183.3

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TGATAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_015183.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055998.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 1, mRNA.

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CloneID	OHu14568
Clone ID Related Accession (Same CDS sequence)	NM_015183.3 , NM_015183.2
Accession Version	NM_015183.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY830839.1 and AC044799.5. On Sep 9, 2009 this sequence version replaced NM_015183.1. Transcript Variant: This variant (1) represents use of an alternate promoter and 5' UTR, uses a downstream start codon, and includes an alternate in-frame exon in the 5' coding region, compared to variant 3. The resulting isoform (a) has a shorter N-terminus and includes an additional segment near the N-terminus, compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY830839.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154361, SAMEA2159607 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TGATAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055998.1
CDS	69..7373
Translation	MDESSILRRRGLQKELSLPRRGSLIDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_015183.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_015183.2

ORF Insert Sequence:

ATGGATGAGT CCAGCATTCT AAGACGAAGA GGGCTCCAGA AGGAGCTGAG TCTCCCCAGA 
AGAGGAAGTT TGATAGATTC CCAGAAGTGG AATTGCTTGG TCAAACGTTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001290227.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277156.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 5, mRNA.

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CloneID	OHu15116
Clone ID Related Accession (Same CDS sequence)	NM_001290227.2 , NM_001290227.1 , XM_006714610.2
Accession Version	NM_001290227.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK296707.1 and AC044799.5. On Jun 2, 2019 this sequence version replaced NM_001290227.1. Transcript Variant: This variant (5) represents use of an alternate promoter, differs in the 5' UTR, uses a downstream start codon, and lacks an alternate in-frame exon compared to variant 3. The encoded protein (isoform e) has a shorter and distinct N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277156.1
CDS	80..7168
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001290227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290227.2

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001290227.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277156.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 5, mRNA.

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CloneID	OHu15116
Clone ID Related Accession (Same CDS sequence)	NM_001290227.2 , NM_001290227.1 , XM_006714610.2
Accession Version	NM_001290227.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008872.6, AK296707.1 and AC044799.5. On Mar 4, 2014 this sequence version replaced XM_006714611.1. Transcript Variant: This variant (5) represents use of an alternate promoter, differs in the 5' UTR, uses a downstream start codon, and lacks an alternate in-frame exon compared to variant 3. The encoded protein (isoform e) has a shorter and distinct N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277156.1
CDS	115..7203
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001290227.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290227.1

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001290226.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277155.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 4, mRNA.

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CloneID	OHu15141
Clone ID Related Accession (Same CDS sequence)	NM_001290226.1 , NM_001290226.2
Accession Version	NM_001290226.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA794310.1, AK299538.1, AB002301.1 and AC044799.5. On Mar 4, 2014 this sequence version replaced XM_006714608.1. Transcript Variant: This variant (4) represents use of an alternate promoter, differs in the 5' UTR, uses a downstream start codon, and has an alternate in-frame exon compared to variant 3. The encoded protein (isoform d) has a shorter and distinct N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGAAAT GACAGGACTG ATGGACGCCG CTGGTCGTTG 
GCTTCTCTCC CTTCCTCTGG CTATGGGACA AACACACCCA GCTCTACGGT CTCTTCATCC 
TGTTCCTCCC AGGAGAAGTT GCATCAGTTA CCATACCAAC CAACACCAGA CGAGTTACAC 
TTCTTATCAA AACATTTCTG TACCACCGAA AGCATCGCCA CTGAGAACAG ATGCAGGAAC 
ACGCCGATGC GCCCCCGTTC CCGAAGTCTG AGCCCTGGAC GTTCTCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC TACAGCTCAG 
ATGGAAGAAC GTCTAAAGGA AATTATCACC AGCTACTCTC CTGACAACGT TCTACCCTTA 
GCAGATGGAG TGCTTAGTTT CACTCACCAC CAGATTATTG AACTGGCTCG AGATTGCTTG 
GATAAATCCC ACCAGGGCCT CATCACCTCA CGATACTTCC TTGAATTACA GCACAAATTA 
GATAAGTTGC TACAGGAGGC TCATGATCGT TCAGAAAGTG GAGAATTGGC ATTTATTAAA 
CAACTAGTTC GAAAGATCCT AATTGTTATT GCCCGCCCTG CTCGGTTATT AGAGTGCCTG 
GAATTTGATC CGGAAGAATT TTACTACCTA TTGGAAGCAG CAGAAGGCCA TGCCAAAGAA 
GGACAGGGTA TTAAAACCGA CATTCCCAGG TACATCATTA GCCAACTGGG ACTCAATAAG 
GATCCCTTGG AAGAAATGGC TCATTTGGGA AACTACGATA GTGGGACAGC AGAAACACCA 
GAAACAGATG AATCAGTGAG TAGCTCTAAT GCCTCCCTGA AACTTCGAAG GAAACCTCGG 
GAAAGTGATT TTGAAACGAT TAAATTGATT AGCAATGGAG CCTATGGGGC AGTCTACTTT 
GTTCGGCATA AAGAATCCCG GCAGAGGTTT GCCATGAAGA AGATTAATAA ACAGAACCTC 
ATCCTTCGAA ACCAGATCCA GCAGGCCTTT GTGGAGCGGG ATATCCTGAC TTTTGCAGAA 
AACCCCTTTG TTGTCAGCAT GTATTGCTCC TTTGAAACAA GGCGCCACTT GTGCATGGTC 
ATGGAATATG TGGAAGGGGG AGACTGTGCT ACTTTAATGA AAAACATGGG TCCTCTCCCT 
GTTGATATGG CCAGAATGTA CTTTGCTGAG ACGGTCTTGG CCTTGGAATA TTTACATAAT 
TATGGAATTG TACACAGGGA TTTGAAACCA GACAACTTGT TGGTTACCTC CATGGGGCAC 
ATAAAGCTGA CAGATTTTGG ATTATCTAAG GTGGGACTAA TGAGCATGAC TACCAACCTT 
TACGAGGGTC ATATTGAGAA GGATGCTAGA GAGTTCCTGG ATAAACAGGT CTGTGGCACA 
CCTGAATACA TTGCACCAGA AGTGATTCTG AGGCAGGGTT ATGGAAAGCC GGTGGACTGG 
TGGGCCATGG GGATTATCCT CTATGAATTT CTGGTTGGAT GCGTGCCATT CTTTGGGGAT 
ACTCCAGAGG AGCTATTTGG ACAAGTCATC AGTGATGAGA TCAACTGGCC TGAGAAGGAT 
GAGGCACCCC CACCTGATGC CCAGGATCTG ATTACCTTAC TCCTCAGGCA GAATCCCCTG 
GAGAGGCTGG GAACAGGTGG TGCATATGAA GTCAAACAGC ATCGATTCTT CCGTTCTTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCA GAATTTATTC CCCAACTGGA ATCTGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCTGAG AAGTATCATC ATATGGAAAC GGAGGAAGAA 
GATGACACAA ATGATGAAGA CTTTAATGTG GAAATAAGGC AGTTTTCTTC ATGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGAATCACTC AGAATTCAGC AGAAGAGAAG 
GAAGACTCTG TGGACAAAAC CAAAAGCACC ACCTTGCCAT CCACAGAAAC ACTGAGCTGG 
AGTTCAGAAT ATTCTGAAAT GCAACAGCTA TCAACATCCA ACTCTTCAGA TACTGAAAGC 
AACAGACATA AACTCAGTTC TGGCCTACTT CCCAAACTGG CTATTTCAAC AGAGGGAGAG 
CAAGATGAAG CTGCCTCCTG CCCTGGAGAC CCCCATGAGG AGCCAGGAAA GCCAGCCCTT 
CCTCCTGAAG AGTGTGCCCA GGAGGAGCCT GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGCTCCACCC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGACGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCGC TTCTCACATG 
GCTCGGCAGC GATTAGAAAG CACAGAAAAA AAGAAAATCT CGGGGAAAGT CACAAAGTCC 
CTCTCTGCCA GTGCTCTTTC CCTCATGATC CCAGGAGATA TGTTTGCTGT TTCCCCTCTG 
GGAAGTCCAA TGTCTCCCCA TTCCCTGTCC TCGGACCCTT CTTCTTCACG AGATTCCTCT 
CCCAGCCGAG ATTCCTCAGC AGCTTCTGCC AGTCCACATC AGCCGATTGT GATCCACAGT 
TCGGGGAAGA ACTACGGCTT TACCATCCGA GCCATCCGGG TGTATGTGGG AGACAGTGAC 
ATCTATACAG TGCACCATAT CGTCTGGAAT GTAGAAGAAG GAAGTCCGGC ATGCCAGGCA 
GGACTGAAGG CTGGAGATCT TATCACTCAC ATCAATGGAG AACCAGTGCA TGGACTTGTC 
CACACAGAAG TTATAGAACT CCTACTGAAG AGTGGGAATA AGGTGTCAAT CACTACTACC 
CCATTTGAAA ACACATCAAT CAAAACTGGA CCAGCCAGGA GAAACAGCTA TAAGAGCCGG 
ATGGTGAGGC GGAGCAAGAA ATCCAAGAAG AAAGAAAGTC TCGAAAGGAG GAGATCTCTT 
TTCAAAAAGC TAGCCAAGCA GCCTTCTCCT TTACTCCACA CCAGCCGAAG TTTCTCCTGC 
TTGAACAGAT CCCTGTCATC GGGTGAGAGC CTCCCAGGTT CCCCCACTCA TAGCTTGTCT 
CCCCGGTCTC CAACACCAAG CTACCGCTCC ACCCCTGACT TCCCATCTGG TACTAATTCC 
TCCCAGAGCA GCTCCCCTAG TTCTAGTGCC CCCAATTCCC CAGCAGGGTC CGGGCACATC 
CGGCCCAGCA CTCTCCACGG TCTTGCACCC AAACTCGGCG GGCAGCGGTA CCGGTCCGGA 
AGGCGAAAGT CCGCCGGCAA CATCCCACTG TCCCCGCTGG CCCGGACGCC CTCTCCAACC 
CCGCAACCCA CCTCCCCGCA GCGGTCACCA TCCCCTCTTC TGGGACACTC ACTGGGCAAT 
TCCAAGATCG CGCAAGCCTT TCCCAGCAAG ATGCACTCCC CGCCCACCAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC GGAGCCCCCC AGGTCCCCGC TGCTCAAGCG CGTGCAGTCC 
GAGGAGAAGC TGTCGCCCTC TTACGGCAGT GACAAGAAGC ACCTGTGCTC CCGCAAGCAC 
AGCCTGGAGG TGACCCAAGA GGAGGTGCAG CGGGAGCAGT CCCAGCGGGA GGCGCCGCTG 
CAGAGCCTGG ATGAGAACGT GTGCGACGTG CCGCCGCTCA GCCGCGCCCG GCCAGTGGAG 
CAAGGCTGCC TGAAACGCCC AGTCTCCCGG AAGGTGGGCC GCCAGGAGTC TGTGGACGAC 
CTGGACCGCG ACAAGCTGAA GGCCAAGGTG GTGGTGAAGA AAGCAGACGG CTTCCCAGAG 
AAACAGGAAT CCCACCAGAA ATCCCATGGA CCCGGGAGTG ATTTGGAAAA CTTTGCTCTG 
TTTAAGCTGG AAGAGAGAGA GAAGAAAGTC TATCCGAAGG CTGTGGAAAG GTCAAGTACT 
TTTGAAAACA AAGCGTCTAT GCAGGAGGCG CCACCGCTGG GCAGCCTGCT GAAGGATGCT 
CTTCACAAGC AGGCCAGCGT GCGCGCCAGC GAGGGTGCGA TGTCGGATGG CCGGGTGCCT 
GCGGAGCACC GCCAGGGTGG CGGGGACTTC AGACGGGCCC CCGCTCCTGG CACCCTCCAG 
GATGGTCTCT GCCACTCCCT CGACAGGGGC ATCTCTGGGA AGGGGGAAGG CACGGAGAAG 
TCCTCCCAGG CCAAGGAGCT TCTCCGATGT GAAAAGTTAG ACAGCAAGCT GGCCAACATC 
GATTACCTCC GAAAGAAAAT GTCACTTGAG GACAAAGAGG ACAACCTCTG CCCTGTGCTG 
AAGCCCAAGA TGACAGCTGG CTCCCACGAA TGCCTGCCAG GGAACCCAGT CCGACCCACG 
GGTGGGCAGC AGGAGCCCCC GCCGGCTTCT GAGAGCCGAG CTTTTGTCAG CAGCACCCAT 
GCAGCTCAGA TGAGTGCCGT CTCTTTTGTT CCCCTCAAGG CCTTAACAGG CCGGGTGGAC 
AGTGGAACGG AGAAGCCTGG CTTGGTTGCT CCTGAGTCCC CTGTTAGGAA GAGCCCCTCC 
GAGTATAAGC TGGAAGGTAG GTCTGTCTCA TGCCTGAAGC CGATCGAGGG CACTCTGGAC 
ATTGCTCTCC TGTCCGGACC TCAGGCCTCC AAGACAGAAC TGCCTTCCCC AGAGTCTGCA 
CAGAGCCCCA GCCCAAGTGG TGACGTGAGG GCCTCTGTGC CACCAGTTCT CCCCAGCAGC 
AGTGGGAAAA AGAACGATAC CACCAGTGCA AGAGAGCTTT CTCCTTCCAG CTTAAAGATG 
AATAAATCCT ACCTGCTGGA GCCTTGGTTC CTGCCCCCCA GCCGAGGTCT CCAGAATTCA 
CCAGCAGTTT CCCTGCCTGA CCCAGAGTTC AAGAGGGACA GGAAAGGTCC CCATCCTACT 
GCCAGGAGCC CTGGAACAGT CATGGAAAGC AATCCCCAAC AGAGAGAGGG CAGCTCCCCT 
AAACACCAAG ACCACACCAC TGACCCCAAG CTTCTGACCT GCCTGGGGCA GAACCTCCAC 
AGCCCTGACC TGGCCAGGCC ACGCTGCCCG CTCCCACCTG AAGCTTCCCC CTCAAGGGAG 
AAGCCAGGCC TGAGGGAATC GTCTGAAAGA GGCCCTCCCA CAGCCAGAAG CGAGCGCTCT 
GCTGCGAGGG CTGACACATG CAGAGAGCCC TCCATGGAAC TGTGCTTTCC AGAAACTGCG 
AAAACCAGTG ACAACTCCAA AAATCTCCTC TCTGTGGGAA GGACCCACCC AGATTTCTAT 
ACACAGACCC AGGCCATGGA GAAAGCATGG GCGCCGGGTG GGAAAACGAA CCACAAAGAT 
GGCCCAGGTG AGGCGAGGCC CCCGCCCAGA GACAACTCCT CTCTGCACTC AGCTGGAATT 
CCCTGTGAGA AGGAGCTGGG CAAGGTGAGG CGTGGCGTGG AACCCAAGCC CGAAGCGCTT 
CTTGCCAGGC GGTCTCTGCA GCCACCTGGA ATTGAGAGTG AGAAGAGTGA AAAGCTCTCC 
AGTTTCCCAT CTTTGCAGAA AGATGGTGCC AAGGAACCTG AAAGGAAGGA GCAGCCTCTA 
CAAAGGCATC CCAGCAGCAT CCCTCCGCCC CCTCTGACGG CCAAAGACCT GTCCAGCCCG 
GCTGCCAGGC AGCATTGCAG TTCCCCAAGC CACGCTTCTG GCAGAGAGCC GGGGGCCAAG 
CCCAGCACTG CAGAGCCCAG CTCGAGCCCC CAGGACCCTC CCAAGCCTGT TGCTGCGCAC 
AGTGAAAGCA GCAGCCACAA GCCCCGGCCT GGCCCTGACC CGGGCCCTCC AAAGACTAAG 
CACCCCGACC GGTCCCTCTC CTCTCAGAAA CCAAGTGTCG GGGCCACAAA GGGCAAAGAG 
CCTGCCACTC AGTCCCTCGG TGGCTCTAGC AGAGAGGGGA AGGGCCACAG TAAGAGTGGG 
CCGGATGTGT TTCCTGCTAC CCCAGGCTCC CAGAACAAAG CCAGCGATGG GATTGGCCAG 
GGAGAAGGTG GGCCCTCTGT CCCACTGCAC ACTGACAGGG CTCCTCTAGA CGCCAAGCCA 
CAACCCACCA GTGGTGGGCG GCCCCTGGAG GTGCTGGAGA AGCCTGTGCA TTTGCCAAGG 
CCGGGACACC CAGGGCCTAG TGAGCCAGCG GACCAGAAAC TGTCCGCTGT TGGTGAAAAG 
CAAACCCTGT CTCCAAAGCA CCCCAAACCA TCCACTGTGA AAGATTGCCC CACCCTGTGC 
AAACAGACAG ACAACAGACA GACAGACAAA AGCCCGAGTC AGCCGGCCGC CAACACCGAC 
AGAAGGGCGG AAGGGAAGAA ATGCACTGAA GCACTTTATG CTCCAGCAGA GGGCGACAAG 
CTCGAGGCCG GCCTTTCCTT TGTGCATAGC GAGAACCGGT TGAAAGGCGC GGAGCGGCCA 
GCCGCGGGGG TGGGGAAGGG CTTCCCTGAG GCCAGAGGGA AAGGGCCCGG TCCCCAGAAG 
CCACCGACGG AGGCAGACAA GCCCAATGGC ATGAAACGGT CCCCCTCAGC CACTGGGCAG 
AGTTCTTTCC GATCCACGGC CCTCCCGGAA AAGTCTCTGA GCTGCTCCTC CAGCTTCCCT 
GAAACCAGGG CCGGAGTTAG AGAGGCCTCT GCAGCCAGCA GCGACACCTC TTCTGCCAAG 
GCCGCCGGGG GCATGCTGGA GCTTCCAGCC CCCAGCAACA GGGACCATAG GAAGGCTCAG 
CCTGCCGGGG AGGGCCGAAC CCACATGACA AAGAGTGACT CCCTGCCCTC CTTCCGGGTC 
TCCACCCTGC CTCTGGAGTC ACACCACCCC GACCCAAACA CCATGGGCGG GGCCAGCCAC 
CGGGACAGGG CTCTCTCGGT GACTGCCACC GTAGGGGAAA CCAAAGGGAA GGACCCTGCC 
CCAGCCCAGC CTCCCCCAGC TAGGAAACAG AACGTGGGCA GAGACGTGAC CAAGCCATCC 
CCAGCCCCAA ACACTGACCG CCCCATCTCT CTTTCTAATG AGAAGGACTT TGTGGTACGG 
CAGAGGCGGG GGAAAGAGAG TTTGCGTAGC AGCCCTCACA AAAAGGCCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277155.1
CDS	379..7632
Translation	MKAQRERLQIPGLTLDCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001290226.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290226.1

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGAAAT GACAGGACTG ATGGACGCCG CTGGTCGTTG 
GCTTCTCTCC CTTCCTCTGG CTATGGGACA AACACACCCA GCTCTACGGT CTCTTCATCC 
TGTTCCTCCC AGGAGAAGTT GCATCAGTTA CCATACCAAC CAACACCAGA CGAGTTACAC 
TTCTTATCAA AACATTTCTG TACCACCGAA AGCATCGCCA CTGAGAACAG ATGCAGGAAC 
ACGCCGATGC GCCCCCGTTC CCGAAGTCTG AGCCCTGGAC GTTCTCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC TACAGCTCAG 
ATGGAAGAAC GTCTAAAGGA AATTATCACC AGCTACTCTC CTGACAACGT TCTACCCTTA 
GCAGATGGAG TGCTTAGTTT CACTCACCAC CAGATTATTG AACTGGCTCG AGATTGCTTG 
GATAAATCCC ACCAGGGCCT CATCACCTCA CGATACTTCC TTGAATTACA GCACAAATTA 
GATAAGTTGC TACAGGAGGC TCATGATCGT TCAGAAAGTG GAGAATTGGC ATTTATTAAA 
CAACTAGTTC GAAAGATCCT AATTGTTATT GCCCGCCCTG CTCGGTTATT AGAGTGCCTG 
GAATTTGATC CGGAAGAATT TTACTACCTA TTGGAAGCAG CAGAAGGCCA TGCCAAAGAA 
GGACAGGGTA TTAAAACCGA CATTCCCAGG TACATCATTA GCCAACTGGG ACTCAATAAG 
GATCCCTTGG AAGAAATGGC TCATTTGGGA AACTACGATA GTGGGACAGC AGAAACACCA 
GAAACAGATG AATCAGTGAG TAGCTCTAAT GCCTCCCTGA AACTTCGAAG GAAACCTCGG 
GAAAGTGATT TTGAAACGAT TAAATTGATT AGCAATGGAG CCTATGGGGC AGTCTACTTT 
GTTCGGCATA AAGAATCCCG GCAGAGGTTT GCCATGAAGA AGATTAATAA ACAGAACCTC 
ATCCTTCGAA ACCAGATCCA GCAGGCCTTT GTGGAGCGGG ATATCCTGAC TTTTGCAGAA 
AACCCCTTTG TTGTCAGCAT GTATTGCTCC TTTGAAACAA GGCGCCACTT GTGCATGGTC 
ATGGAATATG TGGAAGGGGG AGACTGTGCT ACTTTAATGA AAAACATGGG TCCTCTCCCT 
GTTGATATGG CCAGAATGTA CTTTGCTGAG ACGGTCTTGG CCTTGGAATA TTTACATAAT 
TATGGAATTG TACACAGGGA TTTGAAACCA GACAACTTGT TGGTTACCTC CATGGGGCAC 
ATAAAGCTGA CAGATTTTGG ATTATCTAAG GTGGGACTAA TGAGCATGAC TACCAACCTT 
TACGAGGGTC ATATTGAGAA GGATGCTAGA GAGTTCCTGG ATAAACAGGT CTGTGGCACA 
CCTGAATACA TTGCACCAGA AGTGATTCTG AGGCAGGGTT ATGGAAAGCC GGTGGACTGG 
TGGGCCATGG GGATTATCCT CTATGAATTT CTGGTTGGAT GCGTGCCATT CTTTGGGGAT 
ACTCCAGAGG AGCTATTTGG ACAAGTCATC AGTGATGAGA TCAACTGGCC TGAGAAGGAT 
GAGGCACCCC CACCTGATGC CCAGGATCTG ATTACCTTAC TCCTCAGGCA GAATCCCCTG 
GAGAGGCTGG GAACAGGTGG TGCATATGAA GTCAAACAGC ATCGATTCTT CCGTTCTTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCA GAATTTATTC CCCAACTGGA ATCTGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCTGAG AAGTATCATC ATATGGAAAC GGAGGAAGAA 
GATGACACAA ATGATGAAGA CTTTAATGTG GAAATAAGGC AGTTTTCTTC ATGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGAATCACTC AGAATTCAGC AGAAGAGAAG 
GAAGACTCTG TGGACAAAAC CAAAAGCACC ACCTTGCCAT CCACAGAAAC ACTGAGCTGG 
AGTTCAGAAT ATTCTGAAAT GCAACAGCTA TCAACATCCA ACTCTTCAGA TACTGAAAGC 
AACAGACATA AACTCAGTTC TGGCCTACTT CCCAAACTGG CTATTTCAAC AGAGGGAGAG 
CAAGATGAAG CTGCCTCCTG CCCTGGAGAC CCCCATGAGG AGCCAGGAAA GCCAGCCCTT 
CCTCCTGAAG AGTGTGCCCA GGAGGAGCCT GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGCTCCACCC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGACGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCGC TTCTCACATG 
GCTCGGCAGC GATTAGAAAG CACAGAAAAA AAGAAAATCT CGGGGAAAGT CACAAAGTCC 
CTCTCTGCCA GTGCTCTTTC CCTCATGATC CCAGGAGATA TGTTTGCTGT TTCCCCTCTG 
GGAAGTCCAA TGTCTCCCCA TTCCCTGTCC TCGGACCCTT CTTCTTCACG AGATTCCTCT 
CCCAGCCGAG ATTCCTCAGC AGCTTCTGCC AGTCCACATC AGCCGATTGT GATCCACAGT 
TCGGGGAAGA ACTACGGCTT TACCATCCGA GCCATCCGGG TGTATGTGGG AGACAGTGAC 
ATCTATACAG TGCACCATAT CGTCTGGAAT GTAGAAGAAG GAAGTCCGGC ATGCCAGGCA 
GGACTGAAGG CTGGAGATCT TATCACTCAC ATCAATGGAG AACCAGTGCA TGGACTTGTC 
CACACAGAAG TTATAGAACT CCTACTGAAG AGTGGGAATA AGGTGTCAAT CACTACTACC 
CCATTTGAAA ACACATCAAT CAAAACTGGA CCAGCCAGGA GAAACAGCTA TAAGAGCCGG 
ATGGTGAGGC GGAGCAAGAA ATCCAAGAAG AAAGAAAGTC TCGAAAGGAG GAGATCTCTT 
TTCAAAAAGC TAGCCAAGCA GCCTTCTCCT TTACTCCACA CCAGCCGAAG TTTCTCCTGC 
TTGAACAGAT CCCTGTCATC GGGTGAGAGC CTCCCAGGTT CCCCCACTCA TAGCTTGTCT 
CCCCGGTCTC CAACACCAAG CTACCGCTCC ACCCCTGACT TCCCATCTGG TACTAATTCC 
TCCCAGAGCA GCTCCCCTAG TTCTAGTGCC CCCAATTCCC CAGCAGGGTC CGGGCACATC 
CGGCCCAGCA CTCTCCACGG TCTTGCACCC AAACTCGGCG GGCAGCGGTA CCGGTCCGGA 
AGGCGAAAGT CCGCCGGCAA CATCCCACTG TCCCCGCTGG CCCGGACGCC CTCTCCAACC 
CCGCAACCCA CCTCCCCGCA GCGGTCACCA TCCCCTCTTC TGGGACACTC ACTGGGCAAT 
TCCAAGATCG CGCAAGCCTT TCCCAGCAAG ATGCACTCCC CGCCCACCAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC GGAGCCCCCC AGGTCCCCGC TGCTCAAGCG CGTGCAGTCC 
GAGGAGAAGC TGTCGCCCTC TTACGGCAGT GACAAGAAGC ACCTGTGCTC CCGCAAGCAC 
AGCCTGGAGG TGACCCAAGA GGAGGTGCAG CGGGAGCAGT CCCAGCGGGA GGCGCCGCTG 
CAGAGCCTGG ATGAGAACGT GTGCGACGTG CCGCCGCTCA GCCGCGCCCG GCCAGTGGAG 
CAAGGCTGCC TGAAACGCCC AGTCTCCCGG AAGGTGGGCC GCCAGGAGTC TGTGGACGAC 
CTGGACCGCG ACAAGCTGAA GGCCAAGGTG GTGGTGAAGA AAGCAGACGG CTTCCCAGAG 
AAACAGGAAT CCCACCAGAA ATCCCATGGA CCCGGGAGTG ATTTGGAAAA CTTTGCTCTG 
TTTAAGCTGG AAGAGAGAGA GAAGAAAGTC TATCCGAAGG CTGTGGAAAG GTCAAGTACT 
TTTGAAAACA AAGCGTCTAT GCAGGAGGCG CCACCGCTGG GCAGCCTGCT GAAGGATGCT 
CTTCACAAGC AGGCCAGCGT GCGCGCCAGC GAGGGTGCGA TGTCGGATGG CCGGGTGCCT 
GCGGAGCACC GCCAGGGTGG CGGGGACTTC AGACGGGCCC CCGCTCCTGG CACCCTCCAG 
GATGGTCTCT GCCACTCCCT CGACAGGGGC ATCTCTGGGA AGGGGGAAGG CACGGAGAAG 
TCCTCCCAGG CCAAGGAGCT TCTCCGATGT GAAAAGTTAG ACAGCAAGCT GGCCAACATC 
GATTACCTCC GAAAGAAAAT GTCACTTGAG GACAAAGAGG ACAACCTCTG CCCTGTGCTG 
AAGCCCAAGA TGACAGCTGG CTCCCACGAA TGCCTGCCAG GGAACCCAGT CCGACCCACG 
GGTGGGCAGC AGGAGCCCCC GCCGGCTTCT GAGAGCCGAG CTTTTGTCAG CAGCACCCAT 
GCAGCTCAGA TGAGTGCCGT CTCTTTTGTT CCCCTCAAGG CCTTAACAGG CCGGGTGGAC 
AGTGGAACGG AGAAGCCTGG CTTGGTTGCT CCTGAGTCCC CTGTTAGGAA GAGCCCCTCC 
GAGTATAAGC TGGAAGGTAG GTCTGTCTCA TGCCTGAAGC CGATCGAGGG CACTCTGGAC 
ATTGCTCTCC TGTCCGGACC TCAGGCCTCC AAGACAGAAC TGCCTTCCCC AGAGTCTGCA 
CAGAGCCCCA GCCCAAGTGG TGACGTGAGG GCCTCTGTGC CACCAGTTCT CCCCAGCAGC 
AGTGGGAAAA AGAACGATAC CACCAGTGCA AGAGAGCTTT CTCCTTCCAG CTTAAAGATG 
AATAAATCCT ACCTGCTGGA GCCTTGGTTC CTGCCCCCCA GCCGAGGTCT CCAGAATTCA 
CCAGCAGTTT CCCTGCCTGA CCCAGAGTTC AAGAGGGACA GGAAAGGTCC CCATCCTACT 
GCCAGGAGCC CTGGAACAGT CATGGAAAGC AATCCCCAAC AGAGAGAGGG CAGCTCCCCT 
AAACACCAAG ACCACACCAC TGACCCCAAG CTTCTGACCT GCCTGGGGCA GAACCTCCAC 
AGCCCTGACC TGGCCAGGCC ACGCTGCCCG CTCCCACCTG AAGCTTCCCC CTCAAGGGAG 
AAGCCAGGCC TGAGGGAATC GTCTGAAAGA GGCCCTCCCA CAGCCAGAAG CGAGCGCTCT 
GCTGCGAGGG CTGACACATG CAGAGAGCCC TCCATGGAAC TGTGCTTTCC AGAAACTGCG 
AAAACCAGTG ACAACTCCAA AAATCTCCTC TCTGTGGGAA GGACCCACCC AGATTTCTAT 
ACACAGACCC AGGCCATGGA GAAAGCATGG GCGCCGGGTG GGAAAACGAA CCACAAAGAT 
GGCCCAGGTG AGGCGAGGCC CCCGCCCAGA GACAACTCCT CTCTGCACTC AGCTGGAATT 
CCCTGTGAGA AGGAGCTGGG CAAGGTGAGG CGTGGCGTGG AACCCAAGCC CGAAGCGCTT 
CTTGCCAGGC GGTCTCTGCA GCCACCTGGA ATTGAGAGTG AGAAGAGTGA AAAGCTCTCC 
AGTTTCCCAT CTTTGCAGAA AGATGGTGCC AAGGAACCTG AAAGGAAGGA GCAGCCTCTA 
CAAAGGCATC CCAGCAGCAT CCCTCCGCCC CCTCTGACGG CCAAAGACCT GTCCAGCCCG 
GCTGCCAGGC AGCATTGCAG TTCCCCAAGC CACGCTTCTG GCAGAGAGCC GGGGGCCAAG 
CCCAGCACTG CAGAGCCCAG CTCGAGCCCC CAGGACCCTC CCAAGCCTGT TGCTGCGCAC 
AGTGAAAGCA GCAGCCACAA GCCCCGGCCT GGCCCTGACC CGGGCCCTCC AAAGACTAAG 
CACCCCGACC GGTCCCTCTC CTCTCAGAAA CCAAGTGTCG GGGCCACAAA GGGCAAAGAG 
CCTGCCACTC AGTCCCTCGG TGGCTCTAGC AGAGAGGGGA AGGGCCACAG TAAGAGTGGG 
CCGGATGTGT TTCCTGCTAC CCCAGGCTCC CAGAACAAAG CCAGCGATGG GATTGGCCAG 
GGAGAAGGTG GGCCCTCTGT CCCACTGCAC ACTGACAGGG CTCCTCTAGA CGCCAAGCCA 
CAACCCACCA GTGGTGGGCG GCCCCTGGAG GTGCTGGAGA AGCCTGTGCA TTTGCCAAGG 
CCGGGACACC CAGGGCCTAG TGAGCCAGCG GACCAGAAAC TGTCCGCTGT TGGTGAAAAG 
CAAACCCTGT CTCCAAAGCA CCCCAAACCA TCCACTGTGA AAGATTGCCC CACCCTGTGC 
AAACAGACAG ACAACAGACA GACAGACAAA AGCCCGAGTC AGCCGGCCGC CAACACCGAC 
AGAAGGGCGG AAGGGAAGAA ATGCACTGAA GCACTTTATG CTCCAGCAGA GGGCGACAAG 
CTCGAGGCCG GCCTTTCCTT TGTGCATAGC GAGAACCGGT TGAAAGGCGC GGAGCGGCCA 
GCCGCGGGGG TGGGGAAGGG CTTCCCTGAG GCCAGAGGGA AAGGGCCCGG TCCCCAGAAG 
CCACCGACGG AGGCAGACAA GCCCAATGGC ATGAAACGGT CCCCCTCAGC CACTGGGCAG 
AGTTCTTTCC GATCCACGGC CCTCCCGGAA AAGTCTCTGA GCTGCTCCTC CAGCTTCCCT 
GAAACCAGGG CCGGAGTTAG AGAGGCCTCT GCAGCCAGCA GCGACACCTC TTCTGCCAAG 
GCCGCCGGGG GCATGCTGGA GCTTCCAGCC CCCAGCAACA GGGACCATAG GAAGGCTCAG 
CCTGCCGGGG AGGGCCGAAC CCACATGACA AAGAGTGACT CCCTGCCCTC CTTCCGGGTC 
TCCACCCTGC CTCTGGAGTC ACACCACCCC GACCCAAACA CCATGGGCGG GGCCAGCCAC 
CGGGACAGGG CTCTCTCGGT GACTGCCACC GTAGGGGAAA CCAAAGGGAA GGACCCTGCC 
CCAGCCCAGC CTCCCCCAGC TAGGAAACAG AACGTGGGCA GAGACGTGAC CAAGCCATCC 
CCAGCCCCAA ACACTGACCG CCCCATCTCT CTTTCTAATG AGAAGGACTT TGTGGTACGG 
CAGAGGCGGG GGAAAGAGAG TTTGCGTAGC AGCCCTCACA AAAAGGCCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001290226.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277155.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 4, mRNA.

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CloneID	OHu15141
Clone ID Related Accession (Same CDS sequence)	NM_001290226.1 , NM_001290226.2
Accession Version	NM_001290226.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299538.1, AB002301.1 and AC044799.5. On Jun 2, 2019 this sequence version replaced NM_001290226.1. Transcript Variant: This variant (4) represents use of an alternate promoter, differs in the 5' UTR, uses a downstream start codon, and has an alternate in-frame exon compared to variant 3. The encoded protein (isoform d) has a shorter and distinct N-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGAAAT GACAGGACTG ATGGACGCCG CTGGTCGTTG 
GCTTCTCTCC CTTCCTCTGG CTATGGGACA AACACACCCA GCTCTACGGT CTCTTCATCC 
TGTTCCTCCC AGGAGAAGTT GCATCAGTTA CCATACCAAC CAACACCAGA CGAGTTACAC 
TTCTTATCAA AACATTTCTG TACCACCGAA AGCATCGCCA CTGAGAACAG ATGCAGGAAC 
ACGCCGATGC GCCCCCGTTC CCGAAGTCTG AGCCCTGGAC GTTCTCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC TACAGCTCAG 
ATGGAAGAAC GTCTAAAGGA AATTATCACC AGCTACTCTC CTGACAACGT TCTACCCTTA 
GCAGATGGAG TGCTTAGTTT CACTCACCAC CAGATTATTG AACTGGCTCG AGATTGCTTG 
GATAAATCCC ACCAGGGCCT CATCACCTCA CGATACTTCC TTGAATTACA GCACAAATTA 
GATAAGTTGC TACAGGAGGC TCATGATCGT TCAGAAAGTG GAGAATTGGC ATTTATTAAA 
CAACTAGTTC GAAAGATCCT AATTGTTATT GCCCGCCCTG CTCGGTTATT AGAGTGCCTG 
GAATTTGATC CGGAAGAATT TTACTACCTA TTGGAAGCAG CAGAAGGCCA TGCCAAAGAA 
GGACAGGGTA TTAAAACCGA CATTCCCAGG TACATCATTA GCCAACTGGG ACTCAATAAG 
GATCCCTTGG AAGAAATGGC TCATTTGGGA AACTACGATA GTGGGACAGC AGAAACACCA 
GAAACAGATG AATCAGTGAG TAGCTCTAAT GCCTCCCTGA AACTTCGAAG GAAACCTCGG 
GAAAGTGATT TTGAAACGAT TAAATTGATT AGCAATGGAG CCTATGGGGC AGTCTACTTT 
GTTCGGCATA AAGAATCCCG GCAGAGGTTT GCCATGAAGA AGATTAATAA ACAGAACCTC 
ATCCTTCGAA ACCAGATCCA GCAGGCCTTT GTGGAGCGGG ATATCCTGAC TTTTGCAGAA 
AACCCCTTTG TTGTCAGCAT GTATTGCTCC TTTGAAACAA GGCGCCACTT GTGCATGGTC 
ATGGAATATG TGGAAGGGGG AGACTGTGCT ACTTTAATGA AAAACATGGG TCCTCTCCCT 
GTTGATATGG CCAGAATGTA CTTTGCTGAG ACGGTCTTGG CCTTGGAATA TTTACATAAT 
TATGGAATTG TACACAGGGA TTTGAAACCA GACAACTTGT TGGTTACCTC CATGGGGCAC 
ATAAAGCTGA CAGATTTTGG ATTATCTAAG GTGGGACTAA TGAGCATGAC TACCAACCTT 
TACGAGGGTC ATATTGAGAA GGATGCTAGA GAGTTCCTGG ATAAACAGGT CTGTGGCACA 
CCTGAATACA TTGCACCAGA AGTGATTCTG AGGCAGGGTT ATGGAAAGCC GGTGGACTGG 
TGGGCCATGG GGATTATCCT CTATGAATTT CTGGTTGGAT GCGTGCCATT CTTTGGGGAT 
ACTCCAGAGG AGCTATTTGG ACAAGTCATC AGTGATGAGA TCAACTGGCC TGAGAAGGAT 
GAGGCACCCC CACCTGATGC CCAGGATCTG ATTACCTTAC TCCTCAGGCA GAATCCCCTG 
GAGAGGCTGG GAACAGGTGG TGCATATGAA GTCAAACAGC ATCGATTCTT CCGTTCTTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCA GAATTTATTC CCCAACTGGA ATCTGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCTGAG AAGTATCATC ATATGGAAAC GGAGGAAGAA 
GATGACACAA ATGATGAAGA CTTTAATGTG GAAATAAGGC AGTTTTCTTC ATGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGAATCACTC AGAATTCAGC AGAAGAGAAG 
GAAGACTCTG TGGACAAAAC CAAAAGCACC ACCTTGCCAT CCACAGAAAC ACTGAGCTGG 
AGTTCAGAAT ATTCTGAAAT GCAACAGCTA TCAACATCCA ACTCTTCAGA TACTGAAAGC 
AACAGACATA AACTCAGTTC TGGCCTACTT CCCAAACTGG CTATTTCAAC AGAGGGAGAG 
CAAGATGAAG CTGCCTCCTG CCCTGGAGAC CCCCATGAGG AGCCAGGAAA GCCAGCCCTT 
CCTCCTGAAG AGTGTGCCCA GGAGGAGCCT GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGCTCCACCC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGACGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCGC TTCTCACATG 
GCTCGGCAGC GATTAGAAAG CACAGAAAAA AAGAAAATCT CGGGGAAAGT CACAAAGTCC 
CTCTCTGCCA GTGCTCTTTC CCTCATGATC CCAGGAGATA TGTTTGCTGT TTCCCCTCTG 
GGAAGTCCAA TGTCTCCCCA TTCCCTGTCC TCGGACCCTT CTTCTTCACG AGATTCCTCT 
CCCAGCCGAG ATTCCTCAGC AGCTTCTGCC AGTCCACATC AGCCGATTGT GATCCACAGT 
TCGGGGAAGA ACTACGGCTT TACCATCCGA GCCATCCGGG TGTATGTGGG AGACAGTGAC 
ATCTATACAG TGCACCATAT CGTCTGGAAT GTAGAAGAAG GAAGTCCGGC ATGCCAGGCA 
GGACTGAAGG CTGGAGATCT TATCACTCAC ATCAATGGAG AACCAGTGCA TGGACTTGTC 
CACACAGAAG TTATAGAACT CCTACTGAAG AGTGGGAATA AGGTGTCAAT CACTACTACC 
CCATTTGAAA ACACATCAAT CAAAACTGGA CCAGCCAGGA GAAACAGCTA TAAGAGCCGG 
ATGGTGAGGC GGAGCAAGAA ATCCAAGAAG AAAGAAAGTC TCGAAAGGAG GAGATCTCTT 
TTCAAAAAGC TAGCCAAGCA GCCTTCTCCT TTACTCCACA CCAGCCGAAG TTTCTCCTGC 
TTGAACAGAT CCCTGTCATC GGGTGAGAGC CTCCCAGGTT CCCCCACTCA TAGCTTGTCT 
CCCCGGTCTC CAACACCAAG CTACCGCTCC ACCCCTGACT TCCCATCTGG TACTAATTCC 
TCCCAGAGCA GCTCCCCTAG TTCTAGTGCC CCCAATTCCC CAGCAGGGTC CGGGCACATC 
CGGCCCAGCA CTCTCCACGG TCTTGCACCC AAACTCGGCG GGCAGCGGTA CCGGTCCGGA 
AGGCGAAAGT CCGCCGGCAA CATCCCACTG TCCCCGCTGG CCCGGACGCC CTCTCCAACC 
CCGCAACCCA CCTCCCCGCA GCGGTCACCA TCCCCTCTTC TGGGACACTC ACTGGGCAAT 
TCCAAGATCG CGCAAGCCTT TCCCAGCAAG ATGCACTCCC CGCCCACCAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC GGAGCCCCCC AGGTCCCCGC TGCTCAAGCG CGTGCAGTCC 
GAGGAGAAGC TGTCGCCCTC TTACGGCAGT GACAAGAAGC ACCTGTGCTC CCGCAAGCAC 
AGCCTGGAGG TGACCCAAGA GGAGGTGCAG CGGGAGCAGT CCCAGCGGGA GGCGCCGCTG 
CAGAGCCTGG ATGAGAACGT GTGCGACGTG CCGCCGCTCA GCCGCGCCCG GCCAGTGGAG 
CAAGGCTGCC TGAAACGCCC AGTCTCCCGG AAGGTGGGCC GCCAGGAGTC TGTGGACGAC 
CTGGACCGCG ACAAGCTGAA GGCCAAGGTG GTGGTGAAGA AAGCAGACGG CTTCCCAGAG 
AAACAGGAAT CCCACCAGAA ATCCCATGGA CCCGGGAGTG ATTTGGAAAA CTTTGCTCTG 
TTTAAGCTGG AAGAGAGAGA GAAGAAAGTC TATCCGAAGG CTGTGGAAAG GTCAAGTACT 
TTTGAAAACA AAGCGTCTAT GCAGGAGGCG CCACCGCTGG GCAGCCTGCT GAAGGATGCT 
CTTCACAAGC AGGCCAGCGT GCGCGCCAGC GAGGGTGCGA TGTCGGATGG CCGGGTGCCT 
GCGGAGCACC GCCAGGGTGG CGGGGACTTC AGACGGGCCC CCGCTCCTGG CACCCTCCAG 
GATGGTCTCT GCCACTCCCT CGACAGGGGC ATCTCTGGGA AGGGGGAAGG CACGGAGAAG 
TCCTCCCAGG CCAAGGAGCT TCTCCGATGT GAAAAGTTAG ACAGCAAGCT GGCCAACATC 
GATTACCTCC GAAAGAAAAT GTCACTTGAG GACAAAGAGG ACAACCTCTG CCCTGTGCTG 
AAGCCCAAGA TGACAGCTGG CTCCCACGAA TGCCTGCCAG GGAACCCAGT CCGACCCACG 
GGTGGGCAGC AGGAGCCCCC GCCGGCTTCT GAGAGCCGAG CTTTTGTCAG CAGCACCCAT 
GCAGCTCAGA TGAGTGCCGT CTCTTTTGTT CCCCTCAAGG CCTTAACAGG CCGGGTGGAC 
AGTGGAACGG AGAAGCCTGG CTTGGTTGCT CCTGAGTCCC CTGTTAGGAA GAGCCCCTCC 
GAGTATAAGC TGGAAGGTAG GTCTGTCTCA TGCCTGAAGC CGATCGAGGG CACTCTGGAC 
ATTGCTCTCC TGTCCGGACC TCAGGCCTCC AAGACAGAAC TGCCTTCCCC AGAGTCTGCA 
CAGAGCCCCA GCCCAAGTGG TGACGTGAGG GCCTCTGTGC CACCAGTTCT CCCCAGCAGC 
AGTGGGAAAA AGAACGATAC CACCAGTGCA AGAGAGCTTT CTCCTTCCAG CTTAAAGATG 
AATAAATCCT ACCTGCTGGA GCCTTGGTTC CTGCCCCCCA GCCGAGGTCT CCAGAATTCA 
CCAGCAGTTT CCCTGCCTGA CCCAGAGTTC AAGAGGGACA GGAAAGGTCC CCATCCTACT 
GCCAGGAGCC CTGGAACAGT CATGGAAAGC AATCCCCAAC AGAGAGAGGG CAGCTCCCCT 
AAACACCAAG ACCACACCAC TGACCCCAAG CTTCTGACCT GCCTGGGGCA GAACCTCCAC 
AGCCCTGACC TGGCCAGGCC ACGCTGCCCG CTCCCACCTG AAGCTTCCCC CTCAAGGGAG 
AAGCCAGGCC TGAGGGAATC GTCTGAAAGA GGCCCTCCCA CAGCCAGAAG CGAGCGCTCT 
GCTGCGAGGG CTGACACATG CAGAGAGCCC TCCATGGAAC TGTGCTTTCC AGAAACTGCG 
AAAACCAGTG ACAACTCCAA AAATCTCCTC TCTGTGGGAA GGACCCACCC AGATTTCTAT 
ACACAGACCC AGGCCATGGA GAAAGCATGG GCGCCGGGTG GGAAAACGAA CCACAAAGAT 
GGCCCAGGTG AGGCGAGGCC CCCGCCCAGA GACAACTCCT CTCTGCACTC AGCTGGAATT 
CCCTGTGAGA AGGAGCTGGG CAAGGTGAGG CGTGGCGTGG AACCCAAGCC CGAAGCGCTT 
CTTGCCAGGC GGTCTCTGCA GCCACCTGGA ATTGAGAGTG AGAAGAGTGA AAAGCTCTCC 
AGTTTCCCAT CTTTGCAGAA AGATGGTGCC AAGGAACCTG AAAGGAAGGA GCAGCCTCTA 
CAAAGGCATC CCAGCAGCAT CCCTCCGCCC CCTCTGACGG CCAAAGACCT GTCCAGCCCG 
GCTGCCAGGC AGCATTGCAG TTCCCCAAGC CACGCTTCTG GCAGAGAGCC GGGGGCCAAG 
CCCAGCACTG CAGAGCCCAG CTCGAGCCCC CAGGACCCTC CCAAGCCTGT TGCTGCGCAC 
AGTGAAAGCA GCAGCCACAA GCCCCGGCCT GGCCCTGACC CGGGCCCTCC AAAGACTAAG 
CACCCCGACC GGTCCCTCTC CTCTCAGAAA CCAAGTGTCG GGGCCACAAA GGGCAAAGAG 
CCTGCCACTC AGTCCCTCGG TGGCTCTAGC AGAGAGGGGA AGGGCCACAG TAAGAGTGGG 
CCGGATGTGT TTCCTGCTAC CCCAGGCTCC CAGAACAAAG CCAGCGATGG GATTGGCCAG 
GGAGAAGGTG GGCCCTCTGT CCCACTGCAC ACTGACAGGG CTCCTCTAGA CGCCAAGCCA 
CAACCCACCA GTGGTGGGCG GCCCCTGGAG GTGCTGGAGA AGCCTGTGCA TTTGCCAAGG 
CCGGGACACC CAGGGCCTAG TGAGCCAGCG GACCAGAAAC TGTCCGCTGT TGGTGAAAAG 
CAAACCCTGT CTCCAAAGCA CCCCAAACCA TCCACTGTGA AAGATTGCCC CACCCTGTGC 
AAACAGACAG ACAACAGACA GACAGACAAA AGCCCGAGTC AGCCGGCCGC CAACACCGAC 
AGAAGGGCGG AAGGGAAGAA ATGCACTGAA GCACTTTATG CTCCAGCAGA GGGCGACAAG 
CTCGAGGCCG GCCTTTCCTT TGTGCATAGC GAGAACCGGT TGAAAGGCGC GGAGCGGCCA 
GCCGCGGGGG TGGGGAAGGG CTTCCCTGAG GCCAGAGGGA AAGGGCCCGG TCCCCAGAAG 
CCACCGACGG AGGCAGACAA GCCCAATGGC ATGAAACGGT CCCCCTCAGC CACTGGGCAG 
AGTTCTTTCC GATCCACGGC CCTCCCGGAA AAGTCTCTGA GCTGCTCCTC CAGCTTCCCT 
GAAACCAGGG CCGGAGTTAG AGAGGCCTCT GCAGCCAGCA GCGACACCTC TTCTGCCAAG 
GCCGCCGGGG GCATGCTGGA GCTTCCAGCC CCCAGCAACA GGGACCATAG GAAGGCTCAG 
CCTGCCGGGG AGGGCCGAAC CCACATGACA AAGAGTGACT CCCTGCCCTC CTTCCGGGTC 
TCCACCCTGC CTCTGGAGTC ACACCACCCC GACCCAAACA CCATGGGCGG GGCCAGCCAC 
CGGGACAGGG CTCTCTCGGT GACTGCCACC GTAGGGGAAA CCAAAGGGAA GGACCCTGCC 
CCAGCCCAGC CTCCCCCAGC TAGGAAACAG AACGTGGGCA GAGACGTGAC CAAGCCATCC 
CCAGCCCCAA ACACTGACCG CCCCATCTCT CTTTCTAATG AGAAGGACTT TGTGGTACGG 
CAGAGGCGGG GGAAAGAGAG TTTGCGTAGC AGCCCTCACA AAAAGGCCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277155.1
CDS	176..7429
Translation	MKAQRERLQIPGLTLDCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRNDRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001290226.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290226.2

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGAAAT GACAGGACTG ATGGACGCCG CTGGTCGTTG 
GCTTCTCTCC CTTCCTCTGG CTATGGGACA AACACACCCA GCTCTACGGT CTCTTCATCC 
TGTTCCTCCC AGGAGAAGTT GCATCAGTTA CCATACCAAC CAACACCAGA CGAGTTACAC 
TTCTTATCAA AACATTTCTG TACCACCGAA AGCATCGCCA CTGAGAACAG ATGCAGGAAC 
ACGCCGATGC GCCCCCGTTC CCGAAGTCTG AGCCCTGGAC GTTCTCCCGC CTGCTGTGAC 
CATGAAATAA TTATGATGAA CCATGTCTAC AAAGAAAGGT TCCCAAAGGC TACAGCTCAG 
ATGGAAGAAC GTCTAAAGGA AATTATCACC AGCTACTCTC CTGACAACGT TCTACCCTTA 
GCAGATGGAG TGCTTAGTTT CACTCACCAC CAGATTATTG AACTGGCTCG AGATTGCTTG 
GATAAATCCC ACCAGGGCCT CATCACCTCA CGATACTTCC TTGAATTACA GCACAAATTA 
GATAAGTTGC TACAGGAGGC TCATGATCGT TCAGAAAGTG GAGAATTGGC ATTTATTAAA 
CAACTAGTTC GAAAGATCCT AATTGTTATT GCCCGCCCTG CTCGGTTATT AGAGTGCCTG 
GAATTTGATC CGGAAGAATT TTACTACCTA TTGGAAGCAG CAGAAGGCCA TGCCAAAGAA 
GGACAGGGTA TTAAAACCGA CATTCCCAGG TACATCATTA GCCAACTGGG ACTCAATAAG 
GATCCCTTGG AAGAAATGGC TCATTTGGGA AACTACGATA GTGGGACAGC AGAAACACCA 
GAAACAGATG AATCAGTGAG TAGCTCTAAT GCCTCCCTGA AACTTCGAAG GAAACCTCGG 
GAAAGTGATT TTGAAACGAT TAAATTGATT AGCAATGGAG CCTATGGGGC AGTCTACTTT 
GTTCGGCATA AAGAATCCCG GCAGAGGTTT GCCATGAAGA AGATTAATAA ACAGAACCTC 
ATCCTTCGAA ACCAGATCCA GCAGGCCTTT GTGGAGCGGG ATATCCTGAC TTTTGCAGAA 
AACCCCTTTG TTGTCAGCAT GTATTGCTCC TTTGAAACAA GGCGCCACTT GTGCATGGTC 
ATGGAATATG TGGAAGGGGG AGACTGTGCT ACTTTAATGA AAAACATGGG TCCTCTCCCT 
GTTGATATGG CCAGAATGTA CTTTGCTGAG ACGGTCTTGG CCTTGGAATA TTTACATAAT 
TATGGAATTG TACACAGGGA TTTGAAACCA GACAACTTGT TGGTTACCTC CATGGGGCAC 
ATAAAGCTGA CAGATTTTGG ATTATCTAAG GTGGGACTAA TGAGCATGAC TACCAACCTT 
TACGAGGGTC ATATTGAGAA GGATGCTAGA GAGTTCCTGG ATAAACAGGT CTGTGGCACA 
CCTGAATACA TTGCACCAGA AGTGATTCTG AGGCAGGGTT ATGGAAAGCC GGTGGACTGG 
TGGGCCATGG GGATTATCCT CTATGAATTT CTGGTTGGAT GCGTGCCATT CTTTGGGGAT 
ACTCCAGAGG AGCTATTTGG ACAAGTCATC AGTGATGAGA TCAACTGGCC TGAGAAGGAT 
GAGGCACCCC CACCTGATGC CCAGGATCTG ATTACCTTAC TCCTCAGGCA GAATCCCCTG 
GAGAGGCTGG GAACAGGTGG TGCATATGAA GTCAAACAGC ATCGATTCTT CCGTTCTTTA 
GACTGGAACA GTTTGCTGAG ACAGAAGGCA GAATTTATTC CCCAACTGGA ATCTGAGGAT 
GACACAAGTT ATTTTGATAC TCGGTCTGAG AAGTATCATC ATATGGAAAC GGAGGAAGAA 
GATGACACAA ATGATGAAGA CTTTAATGTG GAAATAAGGC AGTTTTCTTC ATGTTCACAC 
AGGTTTTCAA AAGTTTTCAG CAGTATAGAT CGAATCACTC AGAATTCAGC AGAAGAGAAG 
GAAGACTCTG TGGACAAAAC CAAAAGCACC ACCTTGCCAT CCACAGAAAC ACTGAGCTGG 
AGTTCAGAAT ATTCTGAAAT GCAACAGCTA TCAACATCCA ACTCTTCAGA TACTGAAAGC 
AACAGACATA AACTCAGTTC TGGCCTACTT CCCAAACTGG CTATTTCAAC AGAGGGAGAG 
CAAGATGAAG CTGCCTCCTG CCCTGGAGAC CCCCATGAGG AGCCAGGAAA GCCAGCCCTT 
CCTCCTGAAG AGTGTGCCCA GGAGGAGCCT GAGGTCACCA CCCCAGCCAG CACCATCAGC 
AGCTCCACCC TGTCAGTTGG CAGTTTTTCA GAGCACTTGG ATCAGATAAA TGGACGAAGC 
GAGTGTGTGG ACAGTACAGA TAATTCCTCA AAGCCATCCA GTGAACCCGC TTCTCACATG 
GCTCGGCAGC GATTAGAAAG CACAGAAAAA AAGAAAATCT CGGGGAAAGT CACAAAGTCC 
CTCTCTGCCA GTGCTCTTTC CCTCATGATC CCAGGAGATA TGTTTGCTGT TTCCCCTCTG 
GGAAGTCCAA TGTCTCCCCA TTCCCTGTCC TCGGACCCTT CTTCTTCACG AGATTCCTCT 
CCCAGCCGAG ATTCCTCAGC AGCTTCTGCC AGTCCACATC AGCCGATTGT GATCCACAGT 
TCGGGGAAGA ACTACGGCTT TACCATCCGA GCCATCCGGG TGTATGTGGG AGACAGTGAC 
ATCTATACAG TGCACCATAT CGTCTGGAAT GTAGAAGAAG GAAGTCCGGC ATGCCAGGCA 
GGACTGAAGG CTGGAGATCT TATCACTCAC ATCAATGGAG AACCAGTGCA TGGACTTGTC 
CACACAGAAG TTATAGAACT CCTACTGAAG AGTGGGAATA AGGTGTCAAT CACTACTACC 
CCATTTGAAA ACACATCAAT CAAAACTGGA CCAGCCAGGA GAAACAGCTA TAAGAGCCGG 
ATGGTGAGGC GGAGCAAGAA ATCCAAGAAG AAAGAAAGTC TCGAAAGGAG GAGATCTCTT 
TTCAAAAAGC TAGCCAAGCA GCCTTCTCCT TTACTCCACA CCAGCCGAAG TTTCTCCTGC 
TTGAACAGAT CCCTGTCATC GGGTGAGAGC CTCCCAGGTT CCCCCACTCA TAGCTTGTCT 
CCCCGGTCTC CAACACCAAG CTACCGCTCC ACCCCTGACT TCCCATCTGG TACTAATTCC 
TCCCAGAGCA GCTCCCCTAG TTCTAGTGCC CCCAATTCCC CAGCAGGGTC CGGGCACATC 
CGGCCCAGCA CTCTCCACGG TCTTGCACCC AAACTCGGCG GGCAGCGGTA CCGGTCCGGA 
AGGCGAAAGT CCGCCGGCAA CATCCCACTG TCCCCGCTGG CCCGGACGCC CTCTCCAACC 
CCGCAACCCA CCTCCCCGCA GCGGTCACCA TCCCCTCTTC TGGGACACTC ACTGGGCAAT 
TCCAAGATCG CGCAAGCCTT TCCCAGCAAG ATGCACTCCC CGCCCACCAT CGTCAGACAC 
ATCGTGAGGC CCAAGAGTGC GGAGCCCCCC AGGTCCCCGC TGCTCAAGCG CGTGCAGTCC 
GAGGAGAAGC TGTCGCCCTC TTACGGCAGT GACAAGAAGC ACCTGTGCTC CCGCAAGCAC 
AGCCTGGAGG TGACCCAAGA GGAGGTGCAG CGGGAGCAGT CCCAGCGGGA GGCGCCGCTG 
CAGAGCCTGG ATGAGAACGT GTGCGACGTG CCGCCGCTCA GCCGCGCCCG GCCAGTGGAG 
CAAGGCTGCC TGAAACGCCC AGTCTCCCGG AAGGTGGGCC GCCAGGAGTC TGTGGACGAC 
CTGGACCGCG ACAAGCTGAA GGCCAAGGTG GTGGTGAAGA AAGCAGACGG CTTCCCAGAG 
AAACAGGAAT CCCACCAGAA ATCCCATGGA CCCGGGAGTG ATTTGGAAAA CTTTGCTCTG 
TTTAAGCTGG AAGAGAGAGA GAAGAAAGTC TATCCGAAGG CTGTGGAAAG GTCAAGTACT 
TTTGAAAACA AAGCGTCTAT GCAGGAGGCG CCACCGCTGG GCAGCCTGCT GAAGGATGCT 
CTTCACAAGC AGGCCAGCGT GCGCGCCAGC GAGGGTGCGA TGTCGGATGG CCGGGTGCCT 
GCGGAGCACC GCCAGGGTGG CGGGGACTTC AGACGGGCCC CCGCTCCTGG CACCCTCCAG 
GATGGTCTCT GCCACTCCCT CGACAGGGGC ATCTCTGGGA AGGGGGAAGG CACGGAGAAG 
TCCTCCCAGG CCAAGGAGCT TCTCCGATGT GAAAAGTTAG ACAGCAAGCT GGCCAACATC 
GATTACCTCC GAAAGAAAAT GTCACTTGAG GACAAAGAGG ACAACCTCTG CCCTGTGCTG 
AAGCCCAAGA TGACAGCTGG CTCCCACGAA TGCCTGCCAG GGAACCCAGT CCGACCCACG 
GGTGGGCAGC AGGAGCCCCC GCCGGCTTCT GAGAGCCGAG CTTTTGTCAG CAGCACCCAT 
GCAGCTCAGA TGAGTGCCGT CTCTTTTGTT CCCCTCAAGG CCTTAACAGG CCGGGTGGAC 
AGTGGAACGG AGAAGCCTGG CTTGGTTGCT CCTGAGTCCC CTGTTAGGAA GAGCCCCTCC 
GAGTATAAGC TGGAAGGTAG GTCTGTCTCA TGCCTGAAGC CGATCGAGGG CACTCTGGAC 
ATTGCTCTCC TGTCCGGACC TCAGGCCTCC AAGACAGAAC TGCCTTCCCC AGAGTCTGCA 
CAGAGCCCCA GCCCAAGTGG TGACGTGAGG GCCTCTGTGC CACCAGTTCT CCCCAGCAGC 
AGTGGGAAAA AGAACGATAC CACCAGTGCA AGAGAGCTTT CTCCTTCCAG CTTAAAGATG 
AATAAATCCT ACCTGCTGGA GCCTTGGTTC CTGCCCCCCA GCCGAGGTCT CCAGAATTCA 
CCAGCAGTTT CCCTGCCTGA CCCAGAGTTC AAGAGGGACA GGAAAGGTCC CCATCCTACT 
GCCAGGAGCC CTGGAACAGT CATGGAAAGC AATCCCCAAC AGAGAGAGGG CAGCTCCCCT 
AAACACCAAG ACCACACCAC TGACCCCAAG CTTCTGACCT GCCTGGGGCA GAACCTCCAC 
AGCCCTGACC TGGCCAGGCC ACGCTGCCCG CTCCCACCTG AAGCTTCCCC CTCAAGGGAG 
AAGCCAGGCC TGAGGGAATC GTCTGAAAGA GGCCCTCCCA CAGCCAGAAG CGAGCGCTCT 
GCTGCGAGGG CTGACACATG CAGAGAGCCC TCCATGGAAC TGTGCTTTCC AGAAACTGCG 
AAAACCAGTG ACAACTCCAA AAATCTCCTC TCTGTGGGAA GGACCCACCC AGATTTCTAT 
ACACAGACCC AGGCCATGGA GAAAGCATGG GCGCCGGGTG GGAAAACGAA CCACAAAGAT 
GGCCCAGGTG AGGCGAGGCC CCCGCCCAGA GACAACTCCT CTCTGCACTC AGCTGGAATT 
CCCTGTGAGA AGGAGCTGGG CAAGGTGAGG CGTGGCGTGG AACCCAAGCC CGAAGCGCTT 
CTTGCCAGGC GGTCTCTGCA GCCACCTGGA ATTGAGAGTG AGAAGAGTGA AAAGCTCTCC 
AGTTTCCCAT CTTTGCAGAA AGATGGTGCC AAGGAACCTG AAAGGAAGGA GCAGCCTCTA 
CAAAGGCATC CCAGCAGCAT CCCTCCGCCC CCTCTGACGG CCAAAGACCT GTCCAGCCCG 
GCTGCCAGGC AGCATTGCAG TTCCCCAAGC CACGCTTCTG GCAGAGAGCC GGGGGCCAAG 
CCCAGCACTG CAGAGCCCAG CTCGAGCCCC CAGGACCCTC CCAAGCCTGT TGCTGCGCAC 
AGTGAAAGCA GCAGCCACAA GCCCCGGCCT GGCCCTGACC CGGGCCCTCC AAAGACTAAG 
CACCCCGACC GGTCCCTCTC CTCTCAGAAA CCAAGTGTCG GGGCCACAAA GGGCAAAGAG 
CCTGCCACTC AGTCCCTCGG TGGCTCTAGC AGAGAGGGGA AGGGCCACAG TAAGAGTGGG 
CCGGATGTGT TTCCTGCTAC CCCAGGCTCC CAGAACAAAG CCAGCGATGG GATTGGCCAG 
GGAGAAGGTG GGCCCTCTGT CCCACTGCAC ACTGACAGGG CTCCTCTAGA CGCCAAGCCA 
CAACCCACCA GTGGTGGGCG GCCCCTGGAG GTGCTGGAGA AGCCTGTGCA TTTGCCAAGG 
CCGGGACACC CAGGGCCTAG TGAGCCAGCG GACCAGAAAC TGTCCGCTGT TGGTGAAAAG 
CAAACCCTGT CTCCAAAGCA CCCCAAACCA TCCACTGTGA AAGATTGCCC CACCCTGTGC 
AAACAGACAG ACAACAGACA GACAGACAAA AGCCCGAGTC AGCCGGCCGC CAACACCGAC 
AGAAGGGCGG AAGGGAAGAA ATGCACTGAA GCACTTTATG CTCCAGCAGA GGGCGACAAG 
CTCGAGGCCG GCCTTTCCTT TGTGCATAGC GAGAACCGGT TGAAAGGCGC GGAGCGGCCA 
GCCGCGGGGG TGGGGAAGGG CTTCCCTGAG GCCAGAGGGA AAGGGCCCGG TCCCCAGAAG 
CCACCGACGG AGGCAGACAA GCCCAATGGC ATGAAACGGT CCCCCTCAGC CACTGGGCAG 
AGTTCTTTCC GATCCACGGC CCTCCCGGAA AAGTCTCTGA GCTGCTCCTC CAGCTTCCCT 
GAAACCAGGG CCGGAGTTAG AGAGGCCTCT GCAGCCAGCA GCGACACCTC TTCTGCCAAG 
GCCGCCGGGG GCATGCTGGA GCTTCCAGCC CCCAGCAACA GGGACCATAG GAAGGCTCAG 
CCTGCCGGGG AGGGCCGAAC CCACATGACA AAGAGTGACT CCCTGCCCTC CTTCCGGGTC 
TCCACCCTGC CTCTGGAGTC ACACCACCCC GACCCAAACA CCATGGGCGG GGCCAGCCAC 
CGGGACAGGG CTCTCTCGGT GACTGCCACC GTAGGGGAAA CCAAAGGGAA GGACCCTGCC 
CCAGCCCAGC CTCCCCCAGC TAGGAAACAG AACGTGGGCA GAGACGTGAC CAAGCCATCC 
CCAGCCCCAA ACACTGACCG CCCCATCTCT CTTTCTAATG AGAAGGACTT TGTGGTACGG 
CAGAGGCGGG GGAAAGAGAG TTTGCGTAGC AGCCCTCACA AAAAGGCCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001297651.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284580.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 7, mRNA.

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CloneID	OHu39568
Clone ID Related Accession (Same CDS sequence)	NM_001297651.2 , NM_001297651.1 , XM_006714606.3
Accession Version	NM_001297651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299706.1, AK057601.1, AY830839.1, AC044799.5 and BF515864.1. On Jun 2, 2019 this sequence version replaced NM_001297651.1. Transcript Variant: This variant (7) represents use of an alternate promoter, differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate 5' exon, compared to variant 3. The encoded isoform (g) is shorter and has a distinct N-terminus, compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGA CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCGCTTCT CACATGGCTC GGCAGCGATT AGAAAGCACA 
GAAAAAAAGA AAATCTCGGG GAAAGTCACA AAGTCCCTCT CTGCCAGTGC TCTTTCCCTC 
ATGATCCCAG GAGATATGTT TGCTGTTTCC CCTCTGGGAA GTCCAATGTC TCCCCATTCC 
CTGTCCTCGG ACCCTTCTTC TTCACGAGAT TCCTCTCCCA GCCGAGATTC CTCAGCAGCT 
TCTGCCAGTC CACATCAGCC GATTGTGATC CACAGTTCGG GGAAGAACTA CGGCTTTACC 
ATCCGAGCCA TCCGGGTGTA TGTGGGAGAC AGTGACATCT ATACAGTGCA CCATATCGTC 
TGGAATGTAG AAGAAGGAAG TCCGGCATGC CAGGCAGGAC TGAAGGCTGG AGATCTTATC 
ACTCACATCA ATGGAGAACC AGTGCATGGA CTTGTCCACA CAGAAGTTAT AGAACTCCTA 
CTGAAGAGTG GGAATAAGGT GTCAATCACT ACTACCCCAT TTGAAAACAC ATCAATCAAA 
ACTGGACCAG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCC 
AAGAAGAAAG AAAGTCTCGA AAGGAGGAGA TCTCTTTTCA AAAAGCTAGC CAAGCAGCCT 
TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACAGATCCCT GTCATCGGGT 
GAGAGCCTCC CAGGTTCCCC CACTCATAGC TTGTCTCCCC GGTCTCCAAC ACCAAGCTAC 
CGCTCCACCC CTGACTTCCC ATCTGGTACT AATTCCTCCC AGAGCAGCTC CCCTAGTTCT 
AGTGCCCCCA ATTCCCCAGC AGGGTCCGGG CACATCCGGC CCAGCACTCT CCACGGTCTT 
GCACCCAAAC TCGGCGGGCA GCGGTACCGG TCCGGAAGGC GAAAGTCCGC CGGCAACATC 
CCACTGTCCC CGCTGGCCCG GACGCCCTCT CCAACCCCGC AACCCACCTC CCCGCAGCGG 
TCACCATCCC CTCTTCTGGG ACACTCACTG GGCAATTCCA AGATCGCGCA AGCCTTTCCC 
AGCAAGATGC ACTCCCCGCC CACCATCGTC AGACACATCG TGAGGCCCAA GAGTGCGGAG 
CCCCCCAGGT CCCCGCTGCT CAAGCGCGTG CAGTCCGAGG AGAAGCTGTC GCCCTCTTAC 
GGCAGTGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TGGAGGTGAC CCAAGAGGAG 
GTGCAGCGGG AGCAGTCCCA GCGGGAGGCG CCGCTGCAGA GCCTGGATGA GAACGTGTGC 
GACGTGCCGC CGCTCAGCCG CGCCCGGCCA GTGGAGCAAG GCTGCCTGAA ACGCCCAGTC 
TCCCGGAAGG TGGGCCGCCA GGAGTCTGTG GACGACCTGG ACCGCGACAA GCTGAAGGCC 
AAGGTGGTGG TGAAGAAAGC AGACGGCTTC CCAGAGAAAC AGGAATCCCA CCAGAAATCC 
CATGGACCCG GGAGTGATTT GGAAAACTTT GCTCTGTTTA AGCTGGAAGA GAGAGAGAAG 
AAAGTCTATC CGAAGGCTGT GGAAAGGTCA AGTACTTTTG AAAACAAAGC GTCTATGCAG 
GAGGCGCCAC CGCTGGGCAG CCTGCTGAAG GATGCTCTTC ACAAGCAGGC CAGCGTGCGC 
GCCAGCGAGG GTGCGATGTC GGATGGCCGG GTGCCTGCGG AGCACCGCCA GGGTGGCGGG 
GACTTCAGAC GGGCCCCCGC TCCTGGCACC CTCCAGGATG GTCTCTGCCA CTCCCTCGAC 
AGGGGCATCT CTGGGAAGGG GGAAGGCACG GAGAAGTCCT CCCAGGCCAA GGAGCTTCTC 
CGATGTGAAA AGTTAGACAG CAAGCTGGCC AACATCGATT ACCTCCGAAA GAAAATGTCA 
CTTGAGGACA AAGAGGACAA CCTCTGCCCT GTGCTGAAGC CCAAGATGAC AGCTGGCTCC 
CACGAATGCC TGCCAGGGAA CCCAGTCCGA CCCACGGGTG GGCAGCAGGA GCCCCCGCCG 
GCTTCTGAGA GCCGAGCTTT TGTCAGCAGC ACCCATGCAG CTCAGATGAG TGCCGTCTCT 
TTTGTTCCCC TCAAGGCCTT AACAGGCCGG GTGGACAGTG GAACGGAGAA GCCTGGCTTG 
GTTGCTCCTG AGTCCCCTGT TAGGAAGAGC CCCTCCGAGT ATAAGCTGGA AGGTAGGTCT 
GTCTCATGCC TGAAGCCGAT CGAGGGCACT CTGGACATTG CTCTCCTGTC CGGACCTCAG 
GCCTCCAAGA CAGAACTGCC TTCCCCAGAG TCTGCACAGA GCCCCAGCCC AAGTGGTGAC 
GTGAGGGCCT CTGTGCCACC AGTTCTCCCC AGCAGCAGTG GGAAAAAGAA CGATACCACC 
AGTGCAAGAG AGCTTTCTCC TTCCAGCTTA AAGATGAATA AATCCTACCT GCTGGAGCCT 
TGGTTCCTGC CCCCCAGCCG AGGTCTCCAG AATTCACCAG CAGTTTCCCT GCCTGACCCA 
GAGTTCAAGA GGGACAGGAA AGGTCCCCAT CCTACTGCCA GGAGCCCTGG AACAGTCATG 
GAAAGCAATC CCCAACAGAG AGAGGGCAGC TCCCCTAAAC ACCAAGACCA CACCACTGAC 
CCCAAGCTTC TGACCTGCCT GGGGCAGAAC CTCCACAGCC CTGACCTGGC CAGGCCACGC 
TGCCCGCTCC CACCTGAAGC TTCCCCCTCA AGGGAGAAGC CAGGCCTGAG GGAATCGTCT 
GAAAGAGGCC CTCCCACAGC CAGAAGCGAG CGCTCTGCTG CGAGGGCTGA CACATGCAGA 
GAGCCCTCCA TGGAACTGTG CTTTCCAGAA ACTGCGAAAA CCAGTGACAA CTCCAAAAAT 
CTCCTCTCTG TGGGAAGGAC CCACCCAGAT TTCTATACAC AGACCCAGGC CATGGAGAAA 
GCATGGGCGC CGGGTGGGAA AACGAACCAC AAAGATGGCC CAGGTGAGGC GAGGCCCCCG 
CCCAGAGACA ACTCCTCTCT GCACTCAGCT GGAATTCCCT GTGAGAAGGA GCTGGGCAAG 
GTGAGGCGTG GCGTGGAACC CAAGCCCGAA GCGCTTCTTG CCAGGCGGTC TCTGCAGCCA 
CCTGGAATTG AGAGTGAGAA GAGTGAAAAG CTCTCCAGTT TCCCATCTTT GCAGAAAGAT 
GGTGCCAAGG AACCTGAAAG GAAGGAGCAG CCTCTACAAA GGCATCCCAG CAGCATCCCT 
CCGCCCCCTC TGACGGCCAA AGACCTGTCC AGCCCGGCTG CCAGGCAGCA TTGCAGTTCC 
CCAAGCCACG CTTCTGGCAG AGAGCCGGGG GCCAAGCCCA GCACTGCAGA GCCCAGCTCG 
AGCCCCCAGG ACCCTCCCAA GCCTGTTGCT GCGCACAGTG AAAGCAGCAG CCACAAGCCC 
CGGCCTGGCC CTGACCCGGG CCCTCCAAAG ACTAAGCACC CCGACCGGTC CCTCTCCTCT 
CAGAAACCAA GTGTCGGGGC CACAAAGGGC AAAGAGCCTG CCACTCAGTC CCTCGGTGGC 
TCTAGCAGAG AGGGGAAGGG CCACAGTAAG AGTGGGCCGG ATGTGTTTCC TGCTACCCCA 
GGCTCCCAGA ACAAAGCCAG CGATGGGATT GGCCAGGGAG AAGGTGGGCC CTCTGTCCCA 
CTGCACACTG ACAGGGCTCC TCTAGACGCC AAGCCACAAC CCACCAGTGG TGGGCGGCCC 
CTGGAGGTGC TGGAGAAGCC TGTGCATTTG CCAAGGCCGG GACACCCAGG GCCTAGTGAG 
CCAGCGGACC AGAAACTGTC CGCTGTTGGT GAAAAGCAAA CCCTGTCTCC AAAGCACCCC 
AAACCATCCA CTGTGAAAGA TTGCCCCACC CTGTGCAAAC AGACAGACAA CAGACAGACA 
GACAAAAGCC CGAGTCAGCC GGCCGCCAAC ACCGACAGAA GGGCGGAAGG GAAGAAATGC 
ACTGAAGCAC TTTATGCTCC AGCAGAGGGC GACAAGCTCG AGGCCGGCCT TTCCTTTGTG 
CATAGCGAGA ACCGGTTGAA AGGCGCGGAG CGGCCAGCCG CGGGGGTGGG GAAGGGCTTC 
CCTGAGGCCA GAGGGAAAGG GCCCGGTCCC CAGAAGCCAC CGACGGAGGC AGACAAGCCC 
AATGGCATGA AACGGTCCCC CTCAGCCACT GGGCAGAGTT CTTTCCGATC CACGGCCCTC 
CCGGAAAAGT CTCTGAGCTG CTCCTCCAGC TTCCCTGAAA CCAGGGCCGG AGTTAGAGAG 
GCCTCTGCAG CCAGCAGCGA CACCTCTTCT GCCAAGGCCG CCGGGGGCAT GCTGGAGCTT 
CCAGCCCCCA GCAACAGGGA CCATAGGAAG GCTCAGCCTG CCGGGGAGGG CCGAACCCAC 
ATGACAAAGA GTGACTCCCT GCCCTCCTTC CGGGTCTCCA CCCTGCCTCT GGAGTCACAC 
CACCCCGACC CAAACACCAT GGGCGGGGCC AGCCACCGGG ACAGGGCTCT CTCGGTGACT 
GCCACCGTAG GGGAAACCAA AGGGAAGGAC CCTGCCCCAG CCCAGCCTCC CCCAGCTAGG 
AAACAGAACG TGGGCAGAGA CGTGACCAAG CCATCCCCAG CCCCAAACAC TGACCGCCCC 
ATCTCTCTTT CTAATGAGAA GGACTTTGTG GTACGGCAGA GGCGGGGGAA AGAGAGTTTG 
CGTAGCAGCC CTCACAAAAA GGCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284580.1
CDS	340..7629
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001297651.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297651.2

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGA CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCGCTTCT CACATGGCTC GGCAGCGATT AGAAAGCACA 
GAAAAAAAGA AAATCTCGGG GAAAGTCACA AAGTCCCTCT CTGCCAGTGC TCTTTCCCTC 
ATGATCCCAG GAGATATGTT TGCTGTTTCC CCTCTGGGAA GTCCAATGTC TCCCCATTCC 
CTGTCCTCGG ACCCTTCTTC TTCACGAGAT TCCTCTCCCA GCCGAGATTC CTCAGCAGCT 
TCTGCCAGTC CACATCAGCC GATTGTGATC CACAGTTCGG GGAAGAACTA CGGCTTTACC 
ATCCGAGCCA TCCGGGTGTA TGTGGGAGAC AGTGACATCT ATACAGTGCA CCATATCGTC 
TGGAATGTAG AAGAAGGAAG TCCGGCATGC CAGGCAGGAC TGAAGGCTGG AGATCTTATC 
ACTCACATCA ATGGAGAACC AGTGCATGGA CTTGTCCACA CAGAAGTTAT AGAACTCCTA 
CTGAAGAGTG GGAATAAGGT GTCAATCACT ACTACCCCAT TTGAAAACAC ATCAATCAAA 
ACTGGACCAG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCC 
AAGAAGAAAG AAAGTCTCGA AAGGAGGAGA TCTCTTTTCA AAAAGCTAGC CAAGCAGCCT 
TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACAGATCCCT GTCATCGGGT 
GAGAGCCTCC CAGGTTCCCC CACTCATAGC TTGTCTCCCC GGTCTCCAAC ACCAAGCTAC 
CGCTCCACCC CTGACTTCCC ATCTGGTACT AATTCCTCCC AGAGCAGCTC CCCTAGTTCT 
AGTGCCCCCA ATTCCCCAGC AGGGTCCGGG CACATCCGGC CCAGCACTCT CCACGGTCTT 
GCACCCAAAC TCGGCGGGCA GCGGTACCGG TCCGGAAGGC GAAAGTCCGC CGGCAACATC 
CCACTGTCCC CGCTGGCCCG GACGCCCTCT CCAACCCCGC AACCCACCTC CCCGCAGCGG 
TCACCATCCC CTCTTCTGGG ACACTCACTG GGCAATTCCA AGATCGCGCA AGCCTTTCCC 
AGCAAGATGC ACTCCCCGCC CACCATCGTC AGACACATCG TGAGGCCCAA GAGTGCGGAG 
CCCCCCAGGT CCCCGCTGCT CAAGCGCGTG CAGTCCGAGG AGAAGCTGTC GCCCTCTTAC 
GGCAGTGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TGGAGGTGAC CCAAGAGGAG 
GTGCAGCGGG AGCAGTCCCA GCGGGAGGCG CCGCTGCAGA GCCTGGATGA GAACGTGTGC 
GACGTGCCGC CGCTCAGCCG CGCCCGGCCA GTGGAGCAAG GCTGCCTGAA ACGCCCAGTC 
TCCCGGAAGG TGGGCCGCCA GGAGTCTGTG GACGACCTGG ACCGCGACAA GCTGAAGGCC 
AAGGTGGTGG TGAAGAAAGC AGACGGCTTC CCAGAGAAAC AGGAATCCCA CCAGAAATCC 
CATGGACCCG GGAGTGATTT GGAAAACTTT GCTCTGTTTA AGCTGGAAGA GAGAGAGAAG 
AAAGTCTATC CGAAGGCTGT GGAAAGGTCA AGTACTTTTG AAAACAAAGC GTCTATGCAG 
GAGGCGCCAC CGCTGGGCAG CCTGCTGAAG GATGCTCTTC ACAAGCAGGC CAGCGTGCGC 
GCCAGCGAGG GTGCGATGTC GGATGGCCGG GTGCCTGCGG AGCACCGCCA GGGTGGCGGG 
GACTTCAGAC GGGCCCCCGC TCCTGGCACC CTCCAGGATG GTCTCTGCCA CTCCCTCGAC 
AGGGGCATCT CTGGGAAGGG GGAAGGCACG GAGAAGTCCT CCCAGGCCAA GGAGCTTCTC 
CGATGTGAAA AGTTAGACAG CAAGCTGGCC AACATCGATT ACCTCCGAAA GAAAATGTCA 
CTTGAGGACA AAGAGGACAA CCTCTGCCCT GTGCTGAAGC CCAAGATGAC AGCTGGCTCC 
CACGAATGCC TGCCAGGGAA CCCAGTCCGA CCCACGGGTG GGCAGCAGGA GCCCCCGCCG 
GCTTCTGAGA GCCGAGCTTT TGTCAGCAGC ACCCATGCAG CTCAGATGAG TGCCGTCTCT 
TTTGTTCCCC TCAAGGCCTT AACAGGCCGG GTGGACAGTG GAACGGAGAA GCCTGGCTTG 
GTTGCTCCTG AGTCCCCTGT TAGGAAGAGC CCCTCCGAGT ATAAGCTGGA AGGTAGGTCT 
GTCTCATGCC TGAAGCCGAT CGAGGGCACT CTGGACATTG CTCTCCTGTC CGGACCTCAG 
GCCTCCAAGA CAGAACTGCC TTCCCCAGAG TCTGCACAGA GCCCCAGCCC AAGTGGTGAC 
GTGAGGGCCT CTGTGCCACC AGTTCTCCCC AGCAGCAGTG GGAAAAAGAA CGATACCACC 
AGTGCAAGAG AGCTTTCTCC TTCCAGCTTA AAGATGAATA AATCCTACCT GCTGGAGCCT 
TGGTTCCTGC CCCCCAGCCG AGGTCTCCAG AATTCACCAG CAGTTTCCCT GCCTGACCCA 
GAGTTCAAGA GGGACAGGAA AGGTCCCCAT CCTACTGCCA GGAGCCCTGG AACAGTCATG 
GAAAGCAATC CCCAACAGAG AGAGGGCAGC TCCCCTAAAC ACCAAGACCA CACCACTGAC 
CCCAAGCTTC TGACCTGCCT GGGGCAGAAC CTCCACAGCC CTGACCTGGC CAGGCCACGC 
TGCCCGCTCC CACCTGAAGC TTCCCCCTCA AGGGAGAAGC CAGGCCTGAG GGAATCGTCT 
GAAAGAGGCC CTCCCACAGC CAGAAGCGAG CGCTCTGCTG CGAGGGCTGA CACATGCAGA 
GAGCCCTCCA TGGAACTGTG CTTTCCAGAA ACTGCGAAAA CCAGTGACAA CTCCAAAAAT 
CTCCTCTCTG TGGGAAGGAC CCACCCAGAT TTCTATACAC AGACCCAGGC CATGGAGAAA 
GCATGGGCGC CGGGTGGGAA AACGAACCAC AAAGATGGCC CAGGTGAGGC GAGGCCCCCG 
CCCAGAGACA ACTCCTCTCT GCACTCAGCT GGAATTCCCT GTGAGAAGGA GCTGGGCAAG 
GTGAGGCGTG GCGTGGAACC CAAGCCCGAA GCGCTTCTTG CCAGGCGGTC TCTGCAGCCA 
CCTGGAATTG AGAGTGAGAA GAGTGAAAAG CTCTCCAGTT TCCCATCTTT GCAGAAAGAT 
GGTGCCAAGG AACCTGAAAG GAAGGAGCAG CCTCTACAAA GGCATCCCAG CAGCATCCCT 
CCGCCCCCTC TGACGGCCAA AGACCTGTCC AGCCCGGCTG CCAGGCAGCA TTGCAGTTCC 
CCAAGCCACG CTTCTGGCAG AGAGCCGGGG GCCAAGCCCA GCACTGCAGA GCCCAGCTCG 
AGCCCCCAGG ACCCTCCCAA GCCTGTTGCT GCGCACAGTG AAAGCAGCAG CCACAAGCCC 
CGGCCTGGCC CTGACCCGGG CCCTCCAAAG ACTAAGCACC CCGACCGGTC CCTCTCCTCT 
CAGAAACCAA GTGTCGGGGC CACAAAGGGC AAAGAGCCTG CCACTCAGTC CCTCGGTGGC 
TCTAGCAGAG AGGGGAAGGG CCACAGTAAG AGTGGGCCGG ATGTGTTTCC TGCTACCCCA 
GGCTCCCAGA ACAAAGCCAG CGATGGGATT GGCCAGGGAG AAGGTGGGCC CTCTGTCCCA 
CTGCACACTG ACAGGGCTCC TCTAGACGCC AAGCCACAAC CCACCAGTGG TGGGCGGCCC 
CTGGAGGTGC TGGAGAAGCC TGTGCATTTG CCAAGGCCGG GACACCCAGG GCCTAGTGAG 
CCAGCGGACC AGAAACTGTC CGCTGTTGGT GAAAAGCAAA CCCTGTCTCC AAAGCACCCC 
AAACCATCCA CTGTGAAAGA TTGCCCCACC CTGTGCAAAC AGACAGACAA CAGACAGACA 
GACAAAAGCC CGAGTCAGCC GGCCGCCAAC ACCGACAGAA GGGCGGAAGG GAAGAAATGC 
ACTGAAGCAC TTTATGCTCC AGCAGAGGGC GACAAGCTCG AGGCCGGCCT TTCCTTTGTG 
CATAGCGAGA ACCGGTTGAA AGGCGCGGAG CGGCCAGCCG CGGGGGTGGG GAAGGGCTTC 
CCTGAGGCCA GAGGGAAAGG GCCCGGTCCC CAGAAGCCAC CGACGGAGGC AGACAAGCCC 
AATGGCATGA AACGGTCCCC CTCAGCCACT GGGCAGAGTT CTTTCCGATC CACGGCCCTC 
CCGGAAAAGT CTCTGAGCTG CTCCTCCAGC TTCCCTGAAA CCAGGGCCGG AGTTAGAGAG 
GCCTCTGCAG CCAGCAGCGA CACCTCTTCT GCCAAGGCCG CCGGGGGCAT GCTGGAGCTT 
CCAGCCCCCA GCAACAGGGA CCATAGGAAG GCTCAGCCTG CCGGGGAGGG CCGAACCCAC 
ATGACAAAGA GTGACTCCCT GCCCTCCTTC CGGGTCTCCA CCCTGCCTCT GGAGTCACAC 
CACCCCGACC CAAACACCAT GGGCGGGGCC AGCCACCGGG ACAGGGCTCT CTCGGTGACT 
GCCACCGTAG GGGAAACCAA AGGGAAGGAC CCTGCCCCAG CCCAGCCTCC CCCAGCTAGG 
AAACAGAACG TGGGCAGAGA CGTGACCAAG CCATCCCCAG CCCCAAACAC TGACCGCCCC 
ATCTCTCTTT CTAATGAGAA GGACTTTGTG GTACGGCAGA GGCGGGGGAA AGAGAGTTTG 
CGTAGCAGCC CTCACAAAAA GGCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001297651.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001284580.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 7, mRNA.

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CloneID	OHu39568
Clone ID Related Accession (Same CDS sequence)	NM_001297651.2 , NM_001297651.1 , XM_006714606.3
Accession Version	NM_001297651.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK299706.1, AK057601.1, AY830839.1, AC044799.5 and BF515864.1. On Jul 10, 2014 this sequence version replaced XM_006714605.1. Transcript Variant: This variant (7) represents use of an alternate promoter, differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at an alternate 5' exon, compared to variant 3. The encoded isoform (g) is shorter and has a distinct N-terminus, compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END##

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGA CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCGCTTCT CACATGGCTC GGCAGCGATT AGAAAGCACA 
GAAAAAAAGA AAATCTCGGG GAAAGTCACA AAGTCCCTCT CTGCCAGTGC TCTTTCCCTC 
ATGATCCCAG GAGATATGTT TGCTGTTTCC CCTCTGGGAA GTCCAATGTC TCCCCATTCC 
CTGTCCTCGG ACCCTTCTTC TTCACGAGAT TCCTCTCCCA GCCGAGATTC CTCAGCAGCT 
TCTGCCAGTC CACATCAGCC GATTGTGATC CACAGTTCGG GGAAGAACTA CGGCTTTACC 
ATCCGAGCCA TCCGGGTGTA TGTGGGAGAC AGTGACATCT ATACAGTGCA CCATATCGTC 
TGGAATGTAG AAGAAGGAAG TCCGGCATGC CAGGCAGGAC TGAAGGCTGG AGATCTTATC 
ACTCACATCA ATGGAGAACC AGTGCATGGA CTTGTCCACA CAGAAGTTAT AGAACTCCTA 
CTGAAGAGTG GGAATAAGGT GTCAATCACT ACTACCCCAT TTGAAAACAC ATCAATCAAA 
ACTGGACCAG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCC 
AAGAAGAAAG AAAGTCTCGA AAGGAGGAGA TCTCTTTTCA AAAAGCTAGC CAAGCAGCCT 
TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACAGATCCCT GTCATCGGGT 
GAGAGCCTCC CAGGTTCCCC CACTCATAGC TTGTCTCCCC GGTCTCCAAC ACCAAGCTAC 
CGCTCCACCC CTGACTTCCC ATCTGGTACT AATTCCTCCC AGAGCAGCTC CCCTAGTTCT 
AGTGCCCCCA ATTCCCCAGC AGGGTCCGGG CACATCCGGC CCAGCACTCT CCACGGTCTT 
GCACCCAAAC TCGGCGGGCA GCGGTACCGG TCCGGAAGGC GAAAGTCCGC CGGCAACATC 
CCACTGTCCC CGCTGGCCCG GACGCCCTCT CCAACCCCGC AACCCACCTC CCCGCAGCGG 
TCACCATCCC CTCTTCTGGG ACACTCACTG GGCAATTCCA AGATCGCGCA AGCCTTTCCC 
AGCAAGATGC ACTCCCCGCC CACCATCGTC AGACACATCG TGAGGCCCAA GAGTGCGGAG 
CCCCCCAGGT CCCCGCTGCT CAAGCGCGTG CAGTCCGAGG AGAAGCTGTC GCCCTCTTAC 
GGCAGTGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TGGAGGTGAC CCAAGAGGAG 
GTGCAGCGGG AGCAGTCCCA GCGGGAGGCG CCGCTGCAGA GCCTGGATGA GAACGTGTGC 
GACGTGCCGC CGCTCAGCCG CGCCCGGCCA GTGGAGCAAG GCTGCCTGAA ACGCCCAGTC 
TCCCGGAAGG TGGGCCGCCA GGAGTCTGTG GACGACCTGG ACCGCGACAA GCTGAAGGCC 
AAGGTGGTGG TGAAGAAAGC AGACGGCTTC CCAGAGAAAC AGGAATCCCA CCAGAAATCC 
CATGGACCCG GGAGTGATTT GGAAAACTTT GCTCTGTTTA AGCTGGAAGA GAGAGAGAAG 
AAAGTCTATC CGAAGGCTGT GGAAAGGTCA AGTACTTTTG AAAACAAAGC GTCTATGCAG 
GAGGCGCCAC CGCTGGGCAG CCTGCTGAAG GATGCTCTTC ACAAGCAGGC CAGCGTGCGC 
GCCAGCGAGG GTGCGATGTC GGATGGCCGG GTGCCTGCGG AGCACCGCCA GGGTGGCGGG 
GACTTCAGAC GGGCCCCCGC TCCTGGCACC CTCCAGGATG GTCTCTGCCA CTCCCTCGAC 
AGGGGCATCT CTGGGAAGGG GGAAGGCACG GAGAAGTCCT CCCAGGCCAA GGAGCTTCTC 
CGATGTGAAA AGTTAGACAG CAAGCTGGCC AACATCGATT ACCTCCGAAA GAAAATGTCA 
CTTGAGGACA AAGAGGACAA CCTCTGCCCT GTGCTGAAGC CCAAGATGAC AGCTGGCTCC 
CACGAATGCC TGCCAGGGAA CCCAGTCCGA CCCACGGGTG GGCAGCAGGA GCCCCCGCCG 
GCTTCTGAGA GCCGAGCTTT TGTCAGCAGC ACCCATGCAG CTCAGATGAG TGCCGTCTCT 
TTTGTTCCCC TCAAGGCCTT AACAGGCCGG GTGGACAGTG GAACGGAGAA GCCTGGCTTG 
GTTGCTCCTG AGTCCCCTGT TAGGAAGAGC CCCTCCGAGT ATAAGCTGGA AGGTAGGTCT 
GTCTCATGCC TGAAGCCGAT CGAGGGCACT CTGGACATTG CTCTCCTGTC CGGACCTCAG 
GCCTCCAAGA CAGAACTGCC TTCCCCAGAG TCTGCACAGA GCCCCAGCCC AAGTGGTGAC 
GTGAGGGCCT CTGTGCCACC AGTTCTCCCC AGCAGCAGTG GGAAAAAGAA CGATACCACC 
AGTGCAAGAG AGCTTTCTCC TTCCAGCTTA AAGATGAATA AATCCTACCT GCTGGAGCCT 
TGGTTCCTGC CCCCCAGCCG AGGTCTCCAG AATTCACCAG CAGTTTCCCT GCCTGACCCA 
GAGTTCAAGA GGGACAGGAA AGGTCCCCAT CCTACTGCCA GGAGCCCTGG AACAGTCATG 
GAAAGCAATC CCCAACAGAG AGAGGGCAGC TCCCCTAAAC ACCAAGACCA CACCACTGAC 
CCCAAGCTTC TGACCTGCCT GGGGCAGAAC CTCCACAGCC CTGACCTGGC CAGGCCACGC 
TGCCCGCTCC CACCTGAAGC TTCCCCCTCA AGGGAGAAGC CAGGCCTGAG GGAATCGTCT 
GAAAGAGGCC CTCCCACAGC CAGAAGCGAG CGCTCTGCTG CGAGGGCTGA CACATGCAGA 
GAGCCCTCCA TGGAACTGTG CTTTCCAGAA ACTGCGAAAA CCAGTGACAA CTCCAAAAAT 
CTCCTCTCTG TGGGAAGGAC CCACCCAGAT TTCTATACAC AGACCCAGGC CATGGAGAAA 
GCATGGGCGC CGGGTGGGAA AACGAACCAC AAAGATGGCC CAGGTGAGGC GAGGCCCCCG 
CCCAGAGACA ACTCCTCTCT GCACTCAGCT GGAATTCCCT GTGAGAAGGA GCTGGGCAAG 
GTGAGGCGTG GCGTGGAACC CAAGCCCGAA GCGCTTCTTG CCAGGCGGTC TCTGCAGCCA 
CCTGGAATTG AGAGTGAGAA GAGTGAAAAG CTCTCCAGTT TCCCATCTTT GCAGAAAGAT 
GGTGCCAAGG AACCTGAAAG GAAGGAGCAG CCTCTACAAA GGCATCCCAG CAGCATCCCT 
CCGCCCCCTC TGACGGCCAA AGACCTGTCC AGCCCGGCTG CCAGGCAGCA TTGCAGTTCC 
CCAAGCCACG CTTCTGGCAG AGAGCCGGGG GCCAAGCCCA GCACTGCAGA GCCCAGCTCG 
AGCCCCCAGG ACCCTCCCAA GCCTGTTGCT GCGCACAGTG AAAGCAGCAG CCACAAGCCC 
CGGCCTGGCC CTGACCCGGG CCCTCCAAAG ACTAAGCACC CCGACCGGTC CCTCTCCTCT 
CAGAAACCAA GTGTCGGGGC CACAAAGGGC AAAGAGCCTG CCACTCAGTC CCTCGGTGGC 
TCTAGCAGAG AGGGGAAGGG CCACAGTAAG AGTGGGCCGG ATGTGTTTCC TGCTACCCCA 
GGCTCCCAGA ACAAAGCCAG CGATGGGATT GGCCAGGGAG AAGGTGGGCC CTCTGTCCCA 
CTGCACACTG ACAGGGCTCC TCTAGACGCC AAGCCACAAC CCACCAGTGG TGGGCGGCCC 
CTGGAGGTGC TGGAGAAGCC TGTGCATTTG CCAAGGCCGG GACACCCAGG GCCTAGTGAG 
CCAGCGGACC AGAAACTGTC CGCTGTTGGT GAAAAGCAAA CCCTGTCTCC AAAGCACCCC 
AAACCATCCA CTGTGAAAGA TTGCCCCACC CTGTGCAAAC AGACAGACAA CAGACAGACA 
GACAAAAGCC CGAGTCAGCC GGCCGCCAAC ACCGACAGAA GGGCGGAAGG GAAGAAATGC 
ACTGAAGCAC TTTATGCTCC AGCAGAGGGC GACAAGCTCG AGGCCGGCCT TTCCTTTGTG 
CATAGCGAGA ACCGGTTGAA AGGCGCGGAG CGGCCAGCCG CGGGGGTGGG GAAGGGCTTC 
CCTGAGGCCA GAGGGAAAGG GCCCGGTCCC CAGAAGCCAC CGACGGAGGC AGACAAGCCC 
AATGGCATGA AACGGTCCCC CTCAGCCACT GGGCAGAGTT CTTTCCGATC CACGGCCCTC 
CCGGAAAAGT CTCTGAGCTG CTCCTCCAGC TTCCCTGAAA CCAGGGCCGG AGTTAGAGAG 
GCCTCTGCAG CCAGCAGCGA CACCTCTTCT GCCAAGGCCG CCGGGGGCAT GCTGGAGCTT 
CCAGCCCCCA GCAACAGGGA CCATAGGAAG GCTCAGCCTG CCGGGGAGGG CCGAACCCAC 
ATGACAAAGA GTGACTCCCT GCCCTCCTTC CGGGTCTCCA CCCTGCCTCT GGAGTCACAC 
CACCCCGACC CAAACACCAT GGGCGGGGCC AGCCACCGGG ACAGGGCTCT CTCGGTGACT 
GCCACCGTAG GGGAAACCAA AGGGAAGGAC CCTGCCCCAG CCCAGCCTCC CCCAGCTAGG 
AAACAGAACG TGGGCAGAGA CGTGACCAAG CCATCCCCAG CCCCAAACAC TGACCGCCCC 
ATCTCTCTTT CTAATGAGAA GGACTTTGTG GTACGGCAGA GGCGGGGGAA AGAGAGTTTG 
CGTAGCAGCC CTCACAAAAA GGCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001284580.1
CDS	375..7664
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001297651.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001297651.1

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGA CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCGCTTCT CACATGGCTC GGCAGCGATT AGAAAGCACA 
GAAAAAAAGA AAATCTCGGG GAAAGTCACA AAGTCCCTCT CTGCCAGTGC TCTTTCCCTC 
ATGATCCCAG GAGATATGTT TGCTGTTTCC CCTCTGGGAA GTCCAATGTC TCCCCATTCC 
CTGTCCTCGG ACCCTTCTTC TTCACGAGAT TCCTCTCCCA GCCGAGATTC CTCAGCAGCT 
TCTGCCAGTC CACATCAGCC GATTGTGATC CACAGTTCGG GGAAGAACTA CGGCTTTACC 
ATCCGAGCCA TCCGGGTGTA TGTGGGAGAC AGTGACATCT ATACAGTGCA CCATATCGTC 
TGGAATGTAG AAGAAGGAAG TCCGGCATGC CAGGCAGGAC TGAAGGCTGG AGATCTTATC 
ACTCACATCA ATGGAGAACC AGTGCATGGA CTTGTCCACA CAGAAGTTAT AGAACTCCTA 
CTGAAGAGTG GGAATAAGGT GTCAATCACT ACTACCCCAT TTGAAAACAC ATCAATCAAA 
ACTGGACCAG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCC 
AAGAAGAAAG AAAGTCTCGA AAGGAGGAGA TCTCTTTTCA AAAAGCTAGC CAAGCAGCCT 
TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACAGATCCCT GTCATCGGGT 
GAGAGCCTCC CAGGTTCCCC CACTCATAGC TTGTCTCCCC GGTCTCCAAC ACCAAGCTAC 
CGCTCCACCC CTGACTTCCC ATCTGGTACT AATTCCTCCC AGAGCAGCTC CCCTAGTTCT 
AGTGCCCCCA ATTCCCCAGC AGGGTCCGGG CACATCCGGC CCAGCACTCT CCACGGTCTT 
GCACCCAAAC TCGGCGGGCA GCGGTACCGG TCCGGAAGGC GAAAGTCCGC CGGCAACATC 
CCACTGTCCC CGCTGGCCCG GACGCCCTCT CCAACCCCGC AACCCACCTC CCCGCAGCGG 
TCACCATCCC CTCTTCTGGG ACACTCACTG GGCAATTCCA AGATCGCGCA AGCCTTTCCC 
AGCAAGATGC ACTCCCCGCC CACCATCGTC AGACACATCG TGAGGCCCAA GAGTGCGGAG 
CCCCCCAGGT CCCCGCTGCT CAAGCGCGTG CAGTCCGAGG AGAAGCTGTC GCCCTCTTAC 
GGCAGTGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TGGAGGTGAC CCAAGAGGAG 
GTGCAGCGGG AGCAGTCCCA GCGGGAGGCG CCGCTGCAGA GCCTGGATGA GAACGTGTGC 
GACGTGCCGC CGCTCAGCCG CGCCCGGCCA GTGGAGCAAG GCTGCCTGAA ACGCCCAGTC 
TCCCGGAAGG TGGGCCGCCA GGAGTCTGTG GACGACCTGG ACCGCGACAA GCTGAAGGCC 
AAGGTGGTGG TGAAGAAAGC AGACGGCTTC CCAGAGAAAC AGGAATCCCA CCAGAAATCC 
CATGGACCCG GGAGTGATTT GGAAAACTTT GCTCTGTTTA AGCTGGAAGA GAGAGAGAAG 
AAAGTCTATC CGAAGGCTGT GGAAAGGTCA AGTACTTTTG AAAACAAAGC GTCTATGCAG 
GAGGCGCCAC CGCTGGGCAG CCTGCTGAAG GATGCTCTTC ACAAGCAGGC CAGCGTGCGC 
GCCAGCGAGG GTGCGATGTC GGATGGCCGG GTGCCTGCGG AGCACCGCCA GGGTGGCGGG 
GACTTCAGAC GGGCCCCCGC TCCTGGCACC CTCCAGGATG GTCTCTGCCA CTCCCTCGAC 
AGGGGCATCT CTGGGAAGGG GGAAGGCACG GAGAAGTCCT CCCAGGCCAA GGAGCTTCTC 
CGATGTGAAA AGTTAGACAG CAAGCTGGCC AACATCGATT ACCTCCGAAA GAAAATGTCA 
CTTGAGGACA AAGAGGACAA CCTCTGCCCT GTGCTGAAGC CCAAGATGAC AGCTGGCTCC 
CACGAATGCC TGCCAGGGAA CCCAGTCCGA CCCACGGGTG GGCAGCAGGA GCCCCCGCCG 
GCTTCTGAGA GCCGAGCTTT TGTCAGCAGC ACCCATGCAG CTCAGATGAG TGCCGTCTCT 
TTTGTTCCCC TCAAGGCCTT AACAGGCCGG GTGGACAGTG GAACGGAGAA GCCTGGCTTG 
GTTGCTCCTG AGTCCCCTGT TAGGAAGAGC CCCTCCGAGT ATAAGCTGGA AGGTAGGTCT 
GTCTCATGCC TGAAGCCGAT CGAGGGCACT CTGGACATTG CTCTCCTGTC CGGACCTCAG 
GCCTCCAAGA CAGAACTGCC TTCCCCAGAG TCTGCACAGA GCCCCAGCCC AAGTGGTGAC 
GTGAGGGCCT CTGTGCCACC AGTTCTCCCC AGCAGCAGTG GGAAAAAGAA CGATACCACC 
AGTGCAAGAG AGCTTTCTCC TTCCAGCTTA AAGATGAATA AATCCTACCT GCTGGAGCCT 
TGGTTCCTGC CCCCCAGCCG AGGTCTCCAG AATTCACCAG CAGTTTCCCT GCCTGACCCA 
GAGTTCAAGA GGGACAGGAA AGGTCCCCAT CCTACTGCCA GGAGCCCTGG AACAGTCATG 
GAAAGCAATC CCCAACAGAG AGAGGGCAGC TCCCCTAAAC ACCAAGACCA CACCACTGAC 
CCCAAGCTTC TGACCTGCCT GGGGCAGAAC CTCCACAGCC CTGACCTGGC CAGGCCACGC 
TGCCCGCTCC CACCTGAAGC TTCCCCCTCA AGGGAGAAGC CAGGCCTGAG GGAATCGTCT 
GAAAGAGGCC CTCCCACAGC CAGAAGCGAG CGCTCTGCTG CGAGGGCTGA CACATGCAGA 
GAGCCCTCCA TGGAACTGTG CTTTCCAGAA ACTGCGAAAA CCAGTGACAA CTCCAAAAAT 
CTCCTCTCTG TGGGAAGGAC CCACCCAGAT TTCTATACAC AGACCCAGGC CATGGAGAAA 
GCATGGGCGC CGGGTGGGAA AACGAACCAC AAAGATGGCC CAGGTGAGGC GAGGCCCCCG 
CCCAGAGACA ACTCCTCTCT GCACTCAGCT GGAATTCCCT GTGAGAAGGA GCTGGGCAAG 
GTGAGGCGTG GCGTGGAACC CAAGCCCGAA GCGCTTCTTG CCAGGCGGTC TCTGCAGCCA 
CCTGGAATTG AGAGTGAGAA GAGTGAAAAG CTCTCCAGTT TCCCATCTTT GCAGAAAGAT 
GGTGCCAAGG AACCTGAAAG GAAGGAGCAG CCTCTACAAA GGCATCCCAG CAGCATCCCT 
CCGCCCCCTC TGACGGCCAA AGACCTGTCC AGCCCGGCTG CCAGGCAGCA TTGCAGTTCC 
CCAAGCCACG CTTCTGGCAG AGAGCCGGGG GCCAAGCCCA GCACTGCAGA GCCCAGCTCG 
AGCCCCCAGG ACCCTCCCAA GCCTGTTGCT GCGCACAGTG AAAGCAGCAG CCACAAGCCC 
CGGCCTGGCC CTGACCCGGG CCCTCCAAAG ACTAAGCACC CCGACCGGTC CCTCTCCTCT 
CAGAAACCAA GTGTCGGGGC CACAAAGGGC AAAGAGCCTG CCACTCAGTC CCTCGGTGGC 
TCTAGCAGAG AGGGGAAGGG CCACAGTAAG AGTGGGCCGG ATGTGTTTCC TGCTACCCCA 
GGCTCCCAGA ACAAAGCCAG CGATGGGATT GGCCAGGGAG AAGGTGGGCC CTCTGTCCCA 
CTGCACACTG ACAGGGCTCC TCTAGACGCC AAGCCACAAC CCACCAGTGG TGGGCGGCCC 
CTGGAGGTGC TGGAGAAGCC TGTGCATTTG CCAAGGCCGG GACACCCAGG GCCTAGTGAG 
CCAGCGGACC AGAAACTGTC CGCTGTTGGT GAAAAGCAAA CCCTGTCTCC AAAGCACCCC 
AAACCATCCA CTGTGAAAGA TTGCCCCACC CTGTGCAAAC AGACAGACAA CAGACAGACA 
GACAAAAGCC CGAGTCAGCC GGCCGCCAAC ACCGACAGAA GGGCGGAAGG GAAGAAATGC 
ACTGAAGCAC TTTATGCTCC AGCAGAGGGC GACAAGCTCG AGGCCGGCCT TTCCTTTGTG 
CATAGCGAGA ACCGGTTGAA AGGCGCGGAG CGGCCAGCCG CGGGGGTGGG GAAGGGCTTC 
CCTGAGGCCA GAGGGAAAGG GCCCGGTCCC CAGAAGCCAC CGACGGAGGC AGACAAGCCC 
AATGGCATGA AACGGTCCCC CTCAGCCACT GGGCAGAGTT CTTTCCGATC CACGGCCCTC 
CCGGAAAAGT CTCTGAGCTG CTCCTCCAGC TTCCCTGAAA CCAGGGCCGG AGTTAGAGAG 
GCCTCTGCAG CCAGCAGCGA CACCTCTTCT GCCAAGGCCG CCGGGGGCAT GCTGGAGCTT 
CCAGCCCCCA GCAACAGGGA CCATAGGAAG GCTCAGCCTG CCGGGGAGGG CCGAACCCAC 
ATGACAAAGA GTGACTCCCT GCCCTCCTTC CGGGTCTCCA CCCTGCCTCT GGAGTCACAC 
CACCCCGACC CAAACACCAT GGGCGGGGCC AGCCACCGGG ACAGGGCTCT CTCGGTGACT 
GCCACCGTAG GGGAAACCAA AGGGAAGGAC CCTGCCCCAG CCCAGCCTCC CCCAGCTAGG 
AAACAGAACG TGGGCAGAGA CGTGACCAAG CCATCCCCAG CCCCAAACAC TGACCGCCCC 
ATCTCTCTTT CTAATGAGAA GGACTTTGTG GTACGGCAGA GGCGGGGGAA AGAGAGTTTG 
CGTAGCAGCC CTCACAAAAA GGCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_006714610.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714673.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA.

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CloneID	OHu15116
Clone ID Related Accession (Same CDS sequence)	NM_001290227.2 , NM_001290227.1 , XM_006714610.2
Accession Version	XM_006714610.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7089bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714610.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714673.1
CDS	324..7412
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_006714610.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714610.2

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGATATGTTT 
GCTGTTTCCC CTCTGGGAAG TCCAATGTCT CCCCATTCCC TGTCCTCGGA CCCTTCTTCT 
TCACGAGATT CCTCTCCCAG CCGAGATTCC TCAGCAGCTT CTGCCAGTCC ACATCAGCCG 
ATTGTGATCC ACAGTTCGGG GAAGAACTAC GGCTTTACCA TCCGAGCCAT CCGGGTGTAT 
GTGGGAGACA GTGACATCTA TACAGTGCAC CATATCGTCT GGAATGTAGA AGAAGGAAGT 
CCGGCATGCC AGGCAGGACT GAAGGCTGGA GATCTTATCA CTCACATCAA TGGAGAACCA 
GTGCATGGAC TTGTCCACAC AGAAGTTATA GAACTCCTAC TGAAGAGTGG GAATAAGGTG 
TCAATCACTA CTACCCCATT TGAAAACACA TCAATCAAAA CTGGACCAGC CAGGAGAAAC 
AGCTATAAGA GCCGGATGGT GAGGCGGAGC AAGAAATCCA AGAAGAAAGA AAGTCTCGAA 
AGGAGGAGAT CTCTTTTCAA AAAGCTAGCC AAGCAGCCTT CTCCTTTACT CCACACCAGC 
CGAAGTTTCT CCTGCTTGAA CAGATCCCTG TCATCGGGTG AGAGCCTCCC AGGTTCCCCC 
ACTCATAGCT TGTCTCCCCG GTCTCCAACA CCAAGCTACC GCTCCACCCC TGACTTCCCA 
TCTGGTACTA ATTCCTCCCA GAGCAGCTCC CCTAGTTCTA GTGCCCCCAA TTCCCCAGCA 
GGGTCCGGGC ACATCCGGCC CAGCACTCTC CACGGTCTTG CACCCAAACT CGGCGGGCAG 
CGGTACCGGT CCGGAAGGCG AAAGTCCGCC GGCAACATCC CACTGTCCCC GCTGGCCCGG 
ACGCCCTCTC CAACCCCGCA ACCCACCTCC CCGCAGCGGT CACCATCCCC TCTTCTGGGA 
CACTCACTGG GCAATTCCAA GATCGCGCAA GCCTTTCCCA GCAAGATGCA CTCCCCGCCC 
ACCATCGTCA GACACATCGT GAGGCCCAAG AGTGCGGAGC CCCCCAGGTC CCCGCTGCTC 
AAGCGCGTGC AGTCCGAGGA GAAGCTGTCG CCCTCTTACG GCAGTGACAA GAAGCACCTG 
TGCTCCCGCA AGCACAGCCT GGAGGTGACC CAAGAGGAGG TGCAGCGGGA GCAGTCCCAG 
CGGGAGGCGC CGCTGCAGAG CCTGGATGAG AACGTGTGCG ACGTGCCGCC GCTCAGCCGC 
GCCCGGCCAG TGGAGCAAGG CTGCCTGAAA CGCCCAGTCT CCCGGAAGGT GGGCCGCCAG 
GAGTCTGTGG ACGACCTGGA CCGCGACAAG CTGAAGGCCA AGGTGGTGGT GAAGAAAGCA 
GACGGCTTCC CAGAGAAACA GGAATCCCAC CAGAAATCCC ATGGACCCGG GAGTGATTTG 
GAAAACTTTG CTCTGTTTAA GCTGGAAGAG AGAGAGAAGA AAGTCTATCC GAAGGCTGTG 
GAAAGGTCAA GTACTTTTGA AAACAAAGCG TCTATGCAGG AGGCGCCACC GCTGGGCAGC 
CTGCTGAAGG ATGCTCTTCA CAAGCAGGCC AGCGTGCGCG CCAGCGAGGG TGCGATGTCG 
GATGGCCGGG TGCCTGCGGA GCACCGCCAG GGTGGCGGGG ACTTCAGACG GGCCCCCGCT 
CCTGGCACCC TCCAGGATGG TCTCTGCCAC TCCCTCGACA GGGGCATCTC TGGGAAGGGG 
GAAGGCACGG AGAAGTCCTC CCAGGCCAAG GAGCTTCTCC GATGTGAAAA GTTAGACAGC 
AAGCTGGCCA ACATCGATTA CCTCCGAAAG AAAATGTCAC TTGAGGACAA AGAGGACAAC 
CTCTGCCCTG TGCTGAAGCC CAAGATGACA GCTGGCTCCC ACGAATGCCT GCCAGGGAAC 
CCAGTCCGAC CCACGGGTGG GCAGCAGGAG CCCCCGCCGG CTTCTGAGAG CCGAGCTTTT 
GTCAGCAGCA CCCATGCAGC TCAGATGAGT GCCGTCTCTT TTGTTCCCCT CAAGGCCTTA 
ACAGGCCGGG TGGACAGTGG AACGGAGAAG CCTGGCTTGG TTGCTCCTGA GTCCCCTGTT 
AGGAAGAGCC CCTCCGAGTA TAAGCTGGAA GGTAGGTCTG TCTCATGCCT GAAGCCGATC 
GAGGGCACTC TGGACATTGC TCTCCTGTCC GGACCTCAGG CCTCCAAGAC AGAACTGCCT 
TCCCCAGAGT CTGCACAGAG CCCCAGCCCA AGTGGTGACG TGAGGGCCTC TGTGCCACCA 
GTTCTCCCCA GCAGCAGTGG GAAAAAGAAC GATACCACCA GTGCAAGAGA GCTTTCTCCT 
TCCAGCTTAA AGATGAATAA ATCCTACCTG CTGGAGCCTT GGTTCCTGCC CCCCAGCCGA 
GGTCTCCAGA ATTCACCAGC AGTTTCCCTG CCTGACCCAG AGTTCAAGAG GGACAGGAAA 
GGTCCCCATC CTACTGCCAG GAGCCCTGGA ACAGTCATGG AAAGCAATCC CCAACAGAGA 
GAGGGCAGCT CCCCTAAACA CCAAGACCAC ACCACTGACC CCAAGCTTCT GACCTGCCTG 
GGGCAGAACC TCCACAGCCC TGACCTGGCC AGGCCACGCT GCCCGCTCCC ACCTGAAGCT 
TCCCCCTCAA GGGAGAAGCC AGGCCTGAGG GAATCGTCTG AAAGAGGCCC TCCCACAGCC 
AGAAGCGAGC GCTCTGCTGC GAGGGCTGAC ACATGCAGAG AGCCCTCCAT GGAACTGTGC 
TTTCCAGAAA CTGCGAAAAC CAGTGACAAC TCCAAAAATC TCCTCTCTGT GGGAAGGACC 
CACCCAGATT TCTATACACA GACCCAGGCC ATGGAGAAAG CATGGGCGCC GGGTGGGAAA 
ACGAACCACA AAGATGGCCC AGGTGAGGCG AGGCCCCCGC CCAGAGACAA CTCCTCTCTG 
CACTCAGCTG GAATTCCCTG TGAGAAGGAG CTGGGCAAGG TGAGGCGTGG CGTGGAACCC 
AAGCCCGAAG CGCTTCTTGC CAGGCGGTCT CTGCAGCCAC CTGGAATTGA GAGTGAGAAG 
AGTGAAAAGC TCTCCAGTTT CCCATCTTTG CAGAAAGATG GTGCCAAGGA ACCTGAAAGG 
AAGGAGCAGC CTCTACAAAG GCATCCCAGC AGCATCCCTC CGCCCCCTCT GACGGCCAAA 
GACCTGTCCA GCCCGGCTGC CAGGCAGCAT TGCAGTTCCC CAAGCCACGC TTCTGGCAGA 
GAGCCGGGGG CCAAGCCCAG CACTGCAGAG CCCAGCTCGA GCCCCCAGGA CCCTCCCAAG 
CCTGTTGCTG CGCACAGTGA AAGCAGCAGC CACAAGCCCC GGCCTGGCCC TGACCCGGGC 
CCTCCAAAGA CTAAGCACCC CGACCGGTCC CTCTCCTCTC AGAAACCAAG TGTCGGGGCC 
ACAAAGGGCA AAGAGCCTGC CACTCAGTCC CTCGGTGGCT CTAGCAGAGA GGGGAAGGGC 
CACAGTAAGA GTGGGCCGGA TGTGTTTCCT GCTACCCCAG GCTCCCAGAA CAAAGCCAGC 
GATGGGATTG GCCAGGGAGA AGGTGGGCCC TCTGTCCCAC TGCACACTGA CAGGGCTCCT 
CTAGACGCCA AGCCACAACC CACCAGTGGT GGGCGGCCCC TGGAGGTGCT GGAGAAGCCT 
GTGCATTTGC CAAGGCCGGG ACACCCAGGG CCTAGTGAGC CAGCGGACCA GAAACTGTCC 
GCTGTTGGTG AAAAGCAAAC CCTGTCTCCA AAGCACCCCA AACCATCCAC TGTGAAAGAT 
TGCCCCACCC TGTGCAAACA GACAGACAAC AGACAGACAG ACAAAAGCCC GAGTCAGCCG 
GCCGCCAACA CCGACAGAAG GGCGGAAGGG AAGAAATGCA CTGAAGCACT TTATGCTCCA 
GCAGAGGGCG ACAAGCTCGA GGCCGGCCTT TCCTTTGTGC ATAGCGAGAA CCGGTTGAAA 
GGCGCGGAGC GGCCAGCCGC GGGGGTGGGG AAGGGCTTCC CTGAGGCCAG AGGGAAAGGG 
CCCGGTCCCC AGAAGCCACC GACGGAGGCA GACAAGCCCA ATGGCATGAA ACGGTCCCCC 
TCAGCCACTG GGCAGAGTTC TTTCCGATCC ACGGCCCTCC CGGAAAAGTC TCTGAGCTGC 
TCCTCCAGCT TCCCTGAAAC CAGGGCCGGA GTTAGAGAGG CCTCTGCAGC CAGCAGCGAC 
ACCTCTTCTG CCAAGGCCGC CGGGGGCATG CTGGAGCTTC CAGCCCCCAG CAACAGGGAC 
CATAGGAAGG CTCAGCCTGC CGGGGAGGGC CGAACCCACA TGACAAAGAG TGACTCCCTG 
CCCTCCTTCC GGGTCTCCAC CCTGCCTCTG GAGTCACACC ACCCCGACCC AAACACCATG 
GGCGGGGCCA GCCACCGGGA CAGGGCTCTC TCGGTGACTG CCACCGTAGG GGAAACCAAA 
GGGAAGGACC CTGCCCCAGC CCAGCCTCCC CCAGCTAGGA AACAGAACGT GGGCAGAGAC 
GTGACCAAGC CATCCCCAGC CCCAAACACT GACCGCCCCA TCTCTCTTTC TAATGAGAAG 
GACTTTGTGG TACGGCAGAG GCGGGGGAAA GAGAGTTTGC GTAGCAGCCC TCACAAAAAG 
GCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_006714606.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_006714669.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA.

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CloneID	OHu39568
Clone ID Related Accession (Same CDS sequence)	NM_001297651.2 , NM_001297651.1 , XM_006714606.3
Accession Version	XM_006714606.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006714606.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGA CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCGCTTCT CACATGGCTC GGCAGCGATT AGAAAGCACA 
GAAAAAAAGA AAATCTCGGG GAAAGTCACA AAGTCCCTCT CTGCCAGTGC TCTTTCCCTC 
ATGATCCCAG GAGATATGTT TGCTGTTTCC CCTCTGGGAA GTCCAATGTC TCCCCATTCC 
CTGTCCTCGG ACCCTTCTTC TTCACGAGAT TCCTCTCCCA GCCGAGATTC CTCAGCAGCT 
TCTGCCAGTC CACATCAGCC GATTGTGATC CACAGTTCGG GGAAGAACTA CGGCTTTACC 
ATCCGAGCCA TCCGGGTGTA TGTGGGAGAC AGTGACATCT ATACAGTGCA CCATATCGTC 
TGGAATGTAG AAGAAGGAAG TCCGGCATGC CAGGCAGGAC TGAAGGCTGG AGATCTTATC 
ACTCACATCA ATGGAGAACC AGTGCATGGA CTTGTCCACA CAGAAGTTAT AGAACTCCTA 
CTGAAGAGTG GGAATAAGGT GTCAATCACT ACTACCCCAT TTGAAAACAC ATCAATCAAA 
ACTGGACCAG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCC 
AAGAAGAAAG AAAGTCTCGA AAGGAGGAGA TCTCTTTTCA AAAAGCTAGC CAAGCAGCCT 
TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACAGATCCCT GTCATCGGGT 
GAGAGCCTCC CAGGTTCCCC CACTCATAGC TTGTCTCCCC GGTCTCCAAC ACCAAGCTAC 
CGCTCCACCC CTGACTTCCC ATCTGGTACT AATTCCTCCC AGAGCAGCTC CCCTAGTTCT 
AGTGCCCCCA ATTCCCCAGC AGGGTCCGGG CACATCCGGC CCAGCACTCT CCACGGTCTT 
GCACCCAAAC TCGGCGGGCA GCGGTACCGG TCCGGAAGGC GAAAGTCCGC CGGCAACATC 
CCACTGTCCC CGCTGGCCCG GACGCCCTCT CCAACCCCGC AACCCACCTC CCCGCAGCGG 
TCACCATCCC CTCTTCTGGG ACACTCACTG GGCAATTCCA AGATCGCGCA AGCCTTTCCC 
AGCAAGATGC ACTCCCCGCC CACCATCGTC AGACACATCG TGAGGCCCAA GAGTGCGGAG 
CCCCCCAGGT CCCCGCTGCT CAAGCGCGTG CAGTCCGAGG AGAAGCTGTC GCCCTCTTAC 
GGCAGTGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TGGAGGTGAC CCAAGAGGAG 
GTGCAGCGGG AGCAGTCCCA GCGGGAGGCG CCGCTGCAGA GCCTGGATGA GAACGTGTGC 
GACGTGCCGC CGCTCAGCCG CGCCCGGCCA GTGGAGCAAG GCTGCCTGAA ACGCCCAGTC 
TCCCGGAAGG TGGGCCGCCA GGAGTCTGTG GACGACCTGG ACCGCGACAA GCTGAAGGCC 
AAGGTGGTGG TGAAGAAAGC AGACGGCTTC CCAGAGAAAC AGGAATCCCA CCAGAAATCC 
CATGGACCCG GGAGTGATTT GGAAAACTTT GCTCTGTTTA AGCTGGAAGA GAGAGAGAAG 
AAAGTCTATC CGAAGGCTGT GGAAAGGTCA AGTACTTTTG AAAACAAAGC GTCTATGCAG 
GAGGCGCCAC CGCTGGGCAG CCTGCTGAAG GATGCTCTTC ACAAGCAGGC CAGCGTGCGC 
GCCAGCGAGG GTGCGATGTC GGATGGCCGG GTGCCTGCGG AGCACCGCCA GGGTGGCGGG 
GACTTCAGAC GGGCCCCCGC TCCTGGCACC CTCCAGGATG GTCTCTGCCA CTCCCTCGAC 
AGGGGCATCT CTGGGAAGGG GGAAGGCACG GAGAAGTCCT CCCAGGCCAA GGAGCTTCTC 
CGATGTGAAA AGTTAGACAG CAAGCTGGCC AACATCGATT ACCTCCGAAA GAAAATGTCA 
CTTGAGGACA AAGAGGACAA CCTCTGCCCT GTGCTGAAGC CCAAGATGAC AGCTGGCTCC 
CACGAATGCC TGCCAGGGAA CCCAGTCCGA CCCACGGGTG GGCAGCAGGA GCCCCCGCCG 
GCTTCTGAGA GCCGAGCTTT TGTCAGCAGC ACCCATGCAG CTCAGATGAG TGCCGTCTCT 
TTTGTTCCCC TCAAGGCCTT AACAGGCCGG GTGGACAGTG GAACGGAGAA GCCTGGCTTG 
GTTGCTCCTG AGTCCCCTGT TAGGAAGAGC CCCTCCGAGT ATAAGCTGGA AGGTAGGTCT 
GTCTCATGCC TGAAGCCGAT CGAGGGCACT CTGGACATTG CTCTCCTGTC CGGACCTCAG 
GCCTCCAAGA CAGAACTGCC TTCCCCAGAG TCTGCACAGA GCCCCAGCCC AAGTGGTGAC 
GTGAGGGCCT CTGTGCCACC AGTTCTCCCC AGCAGCAGTG GGAAAAAGAA CGATACCACC 
AGTGCAAGAG AGCTTTCTCC TTCCAGCTTA AAGATGAATA AATCCTACCT GCTGGAGCCT 
TGGTTCCTGC CCCCCAGCCG AGGTCTCCAG AATTCACCAG CAGTTTCCCT GCCTGACCCA 
GAGTTCAAGA GGGACAGGAA AGGTCCCCAT CCTACTGCCA GGAGCCCTGG AACAGTCATG 
GAAAGCAATC CCCAACAGAG AGAGGGCAGC TCCCCTAAAC ACCAAGACCA CACCACTGAC 
CCCAAGCTTC TGACCTGCCT GGGGCAGAAC CTCCACAGCC CTGACCTGGC CAGGCCACGC 
TGCCCGCTCC CACCTGAAGC TTCCCCCTCA AGGGAGAAGC CAGGCCTGAG GGAATCGTCT 
GAAAGAGGCC CTCCCACAGC CAGAAGCGAG CGCTCTGCTG CGAGGGCTGA CACATGCAGA 
GAGCCCTCCA TGGAACTGTG CTTTCCAGAA ACTGCGAAAA CCAGTGACAA CTCCAAAAAT 
CTCCTCTCTG TGGGAAGGAC CCACCCAGAT TTCTATACAC AGACCCAGGC CATGGAGAAA 
GCATGGGCGC CGGGTGGGAA AACGAACCAC AAAGATGGCC CAGGTGAGGC GAGGCCCCCG 
CCCAGAGACA ACTCCTCTCT GCACTCAGCT GGAATTCCCT GTGAGAAGGA GCTGGGCAAG 
GTGAGGCGTG GCGTGGAACC CAAGCCCGAA GCGCTTCTTG CCAGGCGGTC TCTGCAGCCA 
CCTGGAATTG AGAGTGAGAA GAGTGAAAAG CTCTCCAGTT TCCCATCTTT GCAGAAAGAT 
GGTGCCAAGG AACCTGAAAG GAAGGAGCAG CCTCTACAAA GGCATCCCAG CAGCATCCCT 
CCGCCCCCTC TGACGGCCAA AGACCTGTCC AGCCCGGCTG CCAGGCAGCA TTGCAGTTCC 
CCAAGCCACG CTTCTGGCAG AGAGCCGGGG GCCAAGCCCA GCACTGCAGA GCCCAGCTCG 
AGCCCCCAGG ACCCTCCCAA GCCTGTTGCT GCGCACAGTG AAAGCAGCAG CCACAAGCCC 
CGGCCTGGCC CTGACCCGGG CCCTCCAAAG ACTAAGCACC CCGACCGGTC CCTCTCCTCT 
CAGAAACCAA GTGTCGGGGC CACAAAGGGC AAAGAGCCTG CCACTCAGTC CCTCGGTGGC 
TCTAGCAGAG AGGGGAAGGG CCACAGTAAG AGTGGGCCGG ATGTGTTTCC TGCTACCCCA 
GGCTCCCAGA ACAAAGCCAG CGATGGGATT GGCCAGGGAG AAGGTGGGCC CTCTGTCCCA 
CTGCACACTG ACAGGGCTCC TCTAGACGCC AAGCCACAAC CCACCAGTGG TGGGCGGCCC 
CTGGAGGTGC TGGAGAAGCC TGTGCATTTG CCAAGGCCGG GACACCCAGG GCCTAGTGAG 
CCAGCGGACC AGAAACTGTC CGCTGTTGGT GAAAAGCAAA CCCTGTCTCC AAAGCACCCC 
AAACCATCCA CTGTGAAAGA TTGCCCCACC CTGTGCAAAC AGACAGACAA CAGACAGACA 
GACAAAAGCC CGAGTCAGCC GGCCGCCAAC ACCGACAGAA GGGCGGAAGG GAAGAAATGC 
ACTGAAGCAC TTTATGCTCC AGCAGAGGGC GACAAGCTCG AGGCCGGCCT TTCCTTTGTG 
CATAGCGAGA ACCGGTTGAA AGGCGCGGAG CGGCCAGCCG CGGGGGTGGG GAAGGGCTTC 
CCTGAGGCCA GAGGGAAAGG GCCCGGTCCC CAGAAGCCAC CGACGGAGGC AGACAAGCCC 
AATGGCATGA AACGGTCCCC CTCAGCCACT GGGCAGAGTT CTTTCCGATC CACGGCCCTC 
CCGGAAAAGT CTCTGAGCTG CTCCTCCAGC TTCCCTGAAA CCAGGGCCGG AGTTAGAGAG 
GCCTCTGCAG CCAGCAGCGA CACCTCTTCT GCCAAGGCCG CCGGGGGCAT GCTGGAGCTT 
CCAGCCCCCA GCAACAGGGA CCATAGGAAG GCTCAGCCTG CCGGGGAGGG CCGAACCCAC 
ATGACAAAGA GTGACTCCCT GCCCTCCTTC CGGGTCTCCA CCCTGCCTCT GGAGTCACAC 
CACCCCGACC CAAACACCAT GGGCGGGGCC AGCCACCGGG ACAGGGCTCT CTCGGTGACT 
GCCACCGTAG GGGAAACCAA AGGGAAGGAC CCTGCCCCAG CCCAGCCTCC CCCAGCTAGG 
AAACAGAACG TGGGCAGAGA CGTGACCAAG CCATCCCCAG CCCCAAACAC TGACCGCCCC 
ATCTCTCTTT CTAATGAGAA GGACTTTGTG GTACGGCAGA GGCGGGGGAA AGAGAGTTTG 
CGTAGCAGCC CTCACAAAAA GGCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006714669.1
CDS	337..7626
Translation	MDMSDPNFWTVLSNFTLPHLRSGNRLRRTQSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_006714606.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006714606.3

ORF Insert Sequence:

ATGGATATGT CTGACCCCAA TTTTTGGACT GTGCTCTCAA ACTTTACTTT GCCTCATTTG 
AGGAGTGGGA ACAGGCTTCG GCGAACACAA AGTTGCCGAA CAAGCAACCG GAAAAGCTTA 
ATAGGCAATG GGCAGTCACC AGCATTGCCT CGACCACACT CACCTCTCTC TGCTCATGCA 
GGAAATAGCC CTCAAGATAG TCCAAGAAAT TTCTCCCCCA GTGCCTCAGC CCATTTTTCA 
TTTGCACGGA GGACTGATGG ACGCCGCTGG TCGTTGGCTT CTCTCCCTTC CTCTGGCTAT 
GGGACAAACA CACCCAGCTC TACGGTCTCT TCATCCTGTT CCTCCCAGGA GAAGTTGCAT 
CAGTTACCAT ACCAACCAAC ACCAGACGAG TTACACTTCT TATCAAAACA TTTCTGTACC 
ACCGAAAGCA TCGCCACTGA GAACAGATGC AGGAACACGC CGATGCGCCC CCGTTCCCGA 
AGTCTGAGCC CTGGACGTTC TCCCGCCTGC TGTGACCATG AAATAATTAT GATGAACCAT 
GTCTACAAAG AAAGGTTCCC AAAGGCTACA GCTCAGATGG AAGAACGTCT AAAGGAAATT 
ATCACCAGCT ACTCTCCTGA CAACGTTCTA CCCTTAGCAG ATGGAGTGCT TAGTTTCACT 
CACCACCAGA TTATTGAACT GGCTCGAGAT TGCTTGGATA AATCCCACCA GGGCCTCATC 
ACCTCACGAT ACTTCCTTGA ATTACAGCAC AAATTAGATA AGTTGCTACA GGAGGCTCAT 
GATCGTTCAG AAAGTGGAGA ATTGGCATTT ATTAAACAAC TAGTTCGAAA GATCCTAATT 
GTTATTGCCC GCCCTGCTCG GTTATTAGAG TGCCTGGAAT TTGATCCGGA AGAATTTTAC 
TACCTATTGG AAGCAGCAGA AGGCCATGCC AAAGAAGGAC AGGGTATTAA AACCGACATT 
CCCAGGTACA TCATTAGCCA ACTGGGACTC AATAAGGATC CCTTGGAAGA AATGGCTCAT 
TTGGGAAACT ACGATAGTGG GACAGCAGAA ACACCAGAAA CAGATGAATC AGTGAGTAGC 
TCTAATGCCT CCCTGAAACT TCGAAGGAAA CCTCGGGAAA GTGATTTTGA AACGATTAAA 
TTGATTAGCA ATGGAGCCTA TGGGGCAGTC TACTTTGTTC GGCATAAAGA ATCCCGGCAG 
AGGTTTGCCA TGAAGAAGAT TAATAAACAG AACCTCATCC TTCGAAACCA GATCCAGCAG 
GCCTTTGTGG AGCGGGATAT CCTGACTTTT GCAGAAAACC CCTTTGTTGT CAGCATGTAT 
TGCTCCTTTG AAACAAGGCG CCACTTGTGC ATGGTCATGG AATATGTGGA AGGGGGAGAC 
TGTGCTACTT TAATGAAAAA CATGGGTCCT CTCCCTGTTG ATATGGCCAG AATGTACTTT 
GCTGAGACGG TCTTGGCCTT GGAATATTTA CATAATTATG GAATTGTACA CAGGGATTTG 
AAACCAGACA ACTTGTTGGT TACCTCCATG GGGCACATAA AGCTGACAGA TTTTGGATTA 
TCTAAGGTGG GACTAATGAG CATGACTACC AACCTTTACG AGGGTCATAT TGAGAAGGAT 
GCTAGAGAGT TCCTGGATAA ACAGGTCTGT GGCACACCTG AATACATTGC ACCAGAAGTG 
ATTCTGAGGC AGGGTTATGG AAAGCCGGTG GACTGGTGGG CCATGGGGAT TATCCTCTAT 
GAATTTCTGG TTGGATGCGT GCCATTCTTT GGGGATACTC CAGAGGAGCT ATTTGGACAA 
GTCATCAGTG ATGAGATCAA CTGGCCTGAG AAGGATGAGG CACCCCCACC TGATGCCCAG 
GATCTGATTA CCTTACTCCT CAGGCAGAAT CCCCTGGAGA GGCTGGGAAC AGGTGGTGCA 
TATGAAGTCA AACAGCATCG ATTCTTCCGT TCTTTAGACT GGAACAGTTT GCTGAGACAG 
AAGGCAGAAT TTATTCCCCA ACTGGAATCT GAGGATGACA CAAGTTATTT TGATACTCGG 
TCTGAGAAGT ATCATCATAT GGAAACGGAG GAAGAAGATG ACACAAATGA TGAAGACTTT 
AATGTGGAAA TAAGGCAGTT TTCTTCATGT TCACACAGGT TTTCAAAAGT TTTCAGCAGT 
ATAGATCGAA TCACTCAGAA TTCAGCAGAA GAGAAGGAAG ACTCTGTGGA CAAAACCAAA 
AGCACCACCT TGCCATCCAC AGAAACACTG AGCTGGAGTT CAGAATATTC TGAAATGCAA 
CAGCTATCAA CATCCAACTC TTCAGATACT GAAAGCAACA GACATAAACT CAGTTCTGGC 
CTACTTCCCA AACTGGCTAT TTCAACAGAG GGAGAGCAAG ATGAAGCTGC CTCCTGCCCT 
GGAGACCCCC ATGAGGAGCC AGGAAAGCCA GCCCTTCCTC CTGAAGAGTG TGCCCAGGAG 
GAGCCTGAGG TCACCACCCC AGCCAGCACC ATCAGCAGCT CCACCCTGTC AGTTGGCAGT 
TTTTCAGAGC ACTTGGATCA GATAAATGGA CGAAGCGAGT GTGTGGACAG TACAGATAAT 
TCCTCAAAGC CATCCAGTGA ACCCGCTTCT CACATGGCTC GGCAGCGATT AGAAAGCACA 
GAAAAAAAGA AAATCTCGGG GAAAGTCACA AAGTCCCTCT CTGCCAGTGC TCTTTCCCTC 
ATGATCCCAG GAGATATGTT TGCTGTTTCC CCTCTGGGAA GTCCAATGTC TCCCCATTCC 
CTGTCCTCGG ACCCTTCTTC TTCACGAGAT TCCTCTCCCA GCCGAGATTC CTCAGCAGCT 
TCTGCCAGTC CACATCAGCC GATTGTGATC CACAGTTCGG GGAAGAACTA CGGCTTTACC 
ATCCGAGCCA TCCGGGTGTA TGTGGGAGAC AGTGACATCT ATACAGTGCA CCATATCGTC 
TGGAATGTAG AAGAAGGAAG TCCGGCATGC CAGGCAGGAC TGAAGGCTGG AGATCTTATC 
ACTCACATCA ATGGAGAACC AGTGCATGGA CTTGTCCACA CAGAAGTTAT AGAACTCCTA 
CTGAAGAGTG GGAATAAGGT GTCAATCACT ACTACCCCAT TTGAAAACAC ATCAATCAAA 
ACTGGACCAG CCAGGAGAAA CAGCTATAAG AGCCGGATGG TGAGGCGGAG CAAGAAATCC 
AAGAAGAAAG AAAGTCTCGA AAGGAGGAGA TCTCTTTTCA AAAAGCTAGC CAAGCAGCCT 
TCTCCTTTAC TCCACACCAG CCGAAGTTTC TCCTGCTTGA ACAGATCCCT GTCATCGGGT 
GAGAGCCTCC CAGGTTCCCC CACTCATAGC TTGTCTCCCC GGTCTCCAAC ACCAAGCTAC 
CGCTCCACCC CTGACTTCCC ATCTGGTACT AATTCCTCCC AGAGCAGCTC CCCTAGTTCT 
AGTGCCCCCA ATTCCCCAGC AGGGTCCGGG CACATCCGGC CCAGCACTCT CCACGGTCTT 
GCACCCAAAC TCGGCGGGCA GCGGTACCGG TCCGGAAGGC GAAAGTCCGC CGGCAACATC 
CCACTGTCCC CGCTGGCCCG GACGCCCTCT CCAACCCCGC AACCCACCTC CCCGCAGCGG 
TCACCATCCC CTCTTCTGGG ACACTCACTG GGCAATTCCA AGATCGCGCA AGCCTTTCCC 
AGCAAGATGC ACTCCCCGCC CACCATCGTC AGACACATCG TGAGGCCCAA GAGTGCGGAG 
CCCCCCAGGT CCCCGCTGCT CAAGCGCGTG CAGTCCGAGG AGAAGCTGTC GCCCTCTTAC 
GGCAGTGACA AGAAGCACCT GTGCTCCCGC AAGCACAGCC TGGAGGTGAC CCAAGAGGAG 
GTGCAGCGGG AGCAGTCCCA GCGGGAGGCG CCGCTGCAGA GCCTGGATGA GAACGTGTGC 
GACGTGCCGC CGCTCAGCCG CGCCCGGCCA GTGGAGCAAG GCTGCCTGAA ACGCCCAGTC 
TCCCGGAAGG TGGGCCGCCA GGAGTCTGTG GACGACCTGG ACCGCGACAA GCTGAAGGCC 
AAGGTGGTGG TGAAGAAAGC AGACGGCTTC CCAGAGAAAC AGGAATCCCA CCAGAAATCC 
CATGGACCCG GGAGTGATTT GGAAAACTTT GCTCTGTTTA AGCTGGAAGA GAGAGAGAAG 
AAAGTCTATC CGAAGGCTGT GGAAAGGTCA AGTACTTTTG AAAACAAAGC GTCTATGCAG 
GAGGCGCCAC CGCTGGGCAG CCTGCTGAAG GATGCTCTTC ACAAGCAGGC CAGCGTGCGC 
GCCAGCGAGG GTGCGATGTC GGATGGCCGG GTGCCTGCGG AGCACCGCCA GGGTGGCGGG 
GACTTCAGAC GGGCCCCCGC TCCTGGCACC CTCCAGGATG GTCTCTGCCA CTCCCTCGAC 
AGGGGCATCT CTGGGAAGGG GGAAGGCACG GAGAAGTCCT CCCAGGCCAA GGAGCTTCTC 
CGATGTGAAA AGTTAGACAG CAAGCTGGCC AACATCGATT ACCTCCGAAA GAAAATGTCA 
CTTGAGGACA AAGAGGACAA CCTCTGCCCT GTGCTGAAGC CCAAGATGAC AGCTGGCTCC 
CACGAATGCC TGCCAGGGAA CCCAGTCCGA CCCACGGGTG GGCAGCAGGA GCCCCCGCCG 
GCTTCTGAGA GCCGAGCTTT TGTCAGCAGC ACCCATGCAG CTCAGATGAG TGCCGTCTCT 
TTTGTTCCCC TCAAGGCCTT AACAGGCCGG GTGGACAGTG GAACGGAGAA GCCTGGCTTG 
GTTGCTCCTG AGTCCCCTGT TAGGAAGAGC CCCTCCGAGT ATAAGCTGGA AGGTAGGTCT 
GTCTCATGCC TGAAGCCGAT CGAGGGCACT CTGGACATTG CTCTCCTGTC CGGACCTCAG 
GCCTCCAAGA CAGAACTGCC TTCCCCAGAG TCTGCACAGA GCCCCAGCCC AAGTGGTGAC 
GTGAGGGCCT CTGTGCCACC AGTTCTCCCC AGCAGCAGTG GGAAAAAGAA CGATACCACC 
AGTGCAAGAG AGCTTTCTCC TTCCAGCTTA AAGATGAATA AATCCTACCT GCTGGAGCCT 
TGGTTCCTGC CCCCCAGCCG AGGTCTCCAG AATTCACCAG CAGTTTCCCT GCCTGACCCA 
GAGTTCAAGA GGGACAGGAA AGGTCCCCAT CCTACTGCCA GGAGCCCTGG AACAGTCATG 
GAAAGCAATC CCCAACAGAG AGAGGGCAGC TCCCCTAAAC ACCAAGACCA CACCACTGAC 
CCCAAGCTTC TGACCTGCCT GGGGCAGAAC CTCCACAGCC CTGACCTGGC CAGGCCACGC 
TGCCCGCTCC CACCTGAAGC TTCCCCCTCA AGGGAGAAGC CAGGCCTGAG GGAATCGTCT 
GAAAGAGGCC CTCCCACAGC CAGAAGCGAG CGCTCTGCTG CGAGGGCTGA CACATGCAGA 
GAGCCCTCCA TGGAACTGTG CTTTCCAGAA ACTGCGAAAA CCAGTGACAA CTCCAAAAAT 
CTCCTCTCTG TGGGAAGGAC CCACCCAGAT TTCTATACAC AGACCCAGGC CATGGAGAAA 
GCATGGGCGC CGGGTGGGAA AACGAACCAC AAAGATGGCC CAGGTGAGGC GAGGCCCCCG 
CCCAGAGACA ACTCCTCTCT GCACTCAGCT GGAATTCCCT GTGAGAAGGA GCTGGGCAAG 
GTGAGGCGTG GCGTGGAACC CAAGCCCGAA GCGCTTCTTG CCAGGCGGTC TCTGCAGCCA 
CCTGGAATTG AGAGTGAGAA GAGTGAAAAG CTCTCCAGTT TCCCATCTTT GCAGAAAGAT 
GGTGCCAAGG AACCTGAAAG GAAGGAGCAG CCTCTACAAA GGCATCCCAG CAGCATCCCT 
CCGCCCCCTC TGACGGCCAA AGACCTGTCC AGCCCGGCTG CCAGGCAGCA TTGCAGTTCC 
CCAAGCCACG CTTCTGGCAG AGAGCCGGGG GCCAAGCCCA GCACTGCAGA GCCCAGCTCG 
AGCCCCCAGG ACCCTCCCAA GCCTGTTGCT GCGCACAGTG AAAGCAGCAG CCACAAGCCC 
CGGCCTGGCC CTGACCCGGG CCCTCCAAAG ACTAAGCACC CCGACCGGTC CCTCTCCTCT 
CAGAAACCAA GTGTCGGGGC CACAAAGGGC AAAGAGCCTG CCACTCAGTC CCTCGGTGGC 
TCTAGCAGAG AGGGGAAGGG CCACAGTAAG AGTGGGCCGG ATGTGTTTCC TGCTACCCCA 
GGCTCCCAGA ACAAAGCCAG CGATGGGATT GGCCAGGGAG AAGGTGGGCC CTCTGTCCCA 
CTGCACACTG ACAGGGCTCC TCTAGACGCC AAGCCACAAC CCACCAGTGG TGGGCGGCCC 
CTGGAGGTGC TGGAGAAGCC TGTGCATTTG CCAAGGCCGG GACACCCAGG GCCTAGTGAG 
CCAGCGGACC AGAAACTGTC CGCTGTTGGT GAAAAGCAAA CCCTGTCTCC AAAGCACCCC 
AAACCATCCA CTGTGAAAGA TTGCCCCACC CTGTGCAAAC AGACAGACAA CAGACAGACA 
GACAAAAGCC CGAGTCAGCC GGCCGCCAAC ACCGACAGAA GGGCGGAAGG GAAGAAATGC 
ACTGAAGCAC TTTATGCTCC AGCAGAGGGC GACAAGCTCG AGGCCGGCCT TTCCTTTGTG 
CATAGCGAGA ACCGGTTGAA AGGCGCGGAG CGGCCAGCCG CGGGGGTGGG GAAGGGCTTC 
CCTGAGGCCA GAGGGAAAGG GCCCGGTCCC CAGAAGCCAC CGACGGAGGC AGACAAGCCC 
AATGGCATGA AACGGTCCCC CTCAGCCACT GGGCAGAGTT CTTTCCGATC CACGGCCCTC 
CCGGAAAAGT CTCTGAGCTG CTCCTCCAGC TTCCCTGAAA CCAGGGCCGG AGTTAGAGAG 
GCCTCTGCAG CCAGCAGCGA CACCTCTTCT GCCAAGGCCG CCGGGGGCAT GCTGGAGCTT 
CCAGCCCCCA GCAACAGGGA CCATAGGAAG GCTCAGCCTG CCGGGGAGGG CCGAACCCAC 
ATGACAAAGA GTGACTCCCT GCCCTCCTTC CGGGTCTCCA CCCTGCCTCT GGAGTCACAC 
CACCCCGACC CAAACACCAT GGGCGGGGCC AGCCACCGGG ACAGGGCTCT CTCGGTGACT 
GCCACCGTAG GGGAAACCAA AGGGAAGGAC CCTGCCCCAG CCCAGCCTCC CCCAGCTAGG 
AAACAGAACG TGGGCAGAGA CGTGACCAAG CCATCCCCAG CCCCAAACAC TGACCGCCCC 
ATCTCTCTTT CTAATGAGAA GGACTTTGTG GTACGGCAGA GGCGGGGGAA AGAGAGTTTG 
CGTAGCAGCC CTCACAAAAA GGCCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_011543384.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541686.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X4, mRNA.

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CloneID	OHu79755
Clone ID Related Accession (Same CDS sequence)	XM_011543384.2
Accession Version	XM_011543384.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7272bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543384.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTATAC CTTTACAGAA TCAAATAATT CCCAGGGATC GTCTGCTATA CAAAGCCCAA 
CAAGCCACTA AAAGTTGCCG AACAAGCAAC CGGAAAAGCT TAATAGGCAA TGGGCAGTCA 
CCAGCATTGC CTCGACCACA CTCACCTCTC TCTGCTCATG CAGGAAATAG CCCTCAAGAT 
AGTCCAAGAA ATTTCTCCCC CAGTGCCTCA GCCCATTTTT CATTTGCACG GAGGACTGAT 
GGACGCCGCT GGTCGTTGGC TTCTCTCCCT TCCTCTGGCT ATGGGACAAA CACACCCAGC 
TCTACGGTCT CTTCATCCTG TTCCTCCCAG GAGAAGTTGC ATCAGTTACC ATACCAACCA 
ACACCAGACG AGTTACACTT CTTATCAAAA CATTTCTGTA CCACCGAAAG CATCGCCACT 
GAGAACAGAT GCAGGAACAC GCCGATGCGC CCCCGTTCCC GAAGTCTGAG CCCTGGACGT 
TCTCCCGCCT GCTGTGACCA TGAAATAATT ATGATGAACC ATGTCTACAA AGAAAGGTTC 
CCAAAGGCTA CAGCTCAGAT GGAAGAACGT CTAAAGGAAA TTATCACCAG CTACTCTCCT 
GACAACGTTC TACCCTTAGC AGATGGAGTG CTTAGTTTCA CTCACCACCA GATTATTGAA 
CTGGCTCGAG ATTGCTTGGA TAAATCCCAC CAGGGCCTCA TCACCTCACG ATACTTCCTT 
GAATTACAGC ACAAATTAGA TAAGTTGCTA CAGGAGGCTC ATGATCGTTC AGAAAGTGGA 
GAATTGGCAT TTATTAAACA ACTAGTTCGA AAGATCCTAA TTGTTATTGC CCGCCCTGCT 
CGGTTATTAG AGTGCCTGGA ATTTGATCCG GAAGAATTTT ACTACCTATT GGAAGCAGCA 
GAAGGCCATG CCAAAGAAGG ACAGGGTATT AAAACCGACA TTCCCAGGTA CATCATTAGC 
CAACTGGGAC TCAATAAGGA TCCCTTGGAA GAAATGGCTC ATTTGGGAAA CTACGATAGT 
GGGACAGCAG AAACACCAGA AACAGATGAA TCAGTGAGTA GCTCTAATGC CTCCCTGAAA 
CTTCGAAGGA AACCTCGGGA AAGTGATTTT GAAACGATTA AATTGATTAG CAATGGAGCC 
TATGGGGCAG TCTACTTTGT TCGGCATAAA GAATCCCGGC AGAGGTTTGC CATGAAGAAG 
ATTAATAAAC AGAACCTCAT CCTTCGAAAC CAGATCCAGC AGGCCTTTGT GGAGCGGGAT 
ATCCTGACTT TTGCAGAAAA CCCCTTTGTT GTCAGCATGT ATTGCTCCTT TGAAACAAGG 
CGCCACTTGT GCATGGTCAT GGAATATGTG GAAGGGGGAG ACTGTGCTAC TTTAATGAAA 
AACATGGGTC CTCTCCCTGT TGATATGGCC AGAATGTACT TTGCTGAGAC GGTCTTGGCC 
TTGGAATATT TACATAATTA TGGAATTGTA CACAGGGATT TGAAACCAGA CAACTTGTTG 
GTTACCTCCA TGGGGCACAT AAAGCTGACA GATTTTGGAT TATCTAAGGT GGGACTAATG 
AGCATGACTA CCAACCTTTA CGAGGGTCAT ATTGAGAAGG ATGCTAGAGA GTTCCTGGAT 
AAACAGGTCT GTGGCACACC TGAATACATT GCACCAGAAG TGATTCTGAG GCAGGGTTAT 
GGAAAGCCGG TGGACTGGTG GGCCATGGGG ATTATCCTCT ATGAATTTCT GGTTGGATGC 
GTGCCATTCT TTGGGGATAC TCCAGAGGAG CTATTTGGAC AAGTCATCAG TGATGAGATC 
AACTGGCCTG AGAAGGATGA GGCACCCCCA CCTGATGCCC AGGATCTGAT TACCTTACTC 
CTCAGGCAGA ATCCCCTGGA GAGGCTGGGA ACAGGTGGTG CATATGAAGT CAAACAGCAT 
CGATTCTTCC GTTCTTTAGA CTGGAACAGT TTGCTGAGAC AGAAGGCAGA ATTTATTCCC 
CAACTGGAAT CTGAGGATGA CACAAGTTAT TTTGATACTC GGTCTGAGAA GTATCATCAT 
ATGGAAACGG AGGAAGAAGA TGACACAAAT GATGAAGACT TTAATGTGGA AATAAGGCAG 
TTTTCTTCAT GTTCACACAG GTTTTCAAAA GTTTTCAGCA GTATAGATCG AATCACTCAG 
AATTCAGCAG AAGAGAAGGA AGACTCTGTG GACAAAACCA AAAGCACCAC CTTGCCATCC 
ACAGAAACAC TGAGCTGGAG TTCAGAATAT TCTGAAATGC AACAGCTATC AACATCCAAC 
TCTTCAGATA CTGAAAGCAA CAGACATAAA CTCAGTTCTG GCCTACTTCC CAAACTGGCT 
ATTTCAACAG AGGGAGAGCA AGATGAAGCT GCCTCCTGCC CTGGAGACCC CCATGAGGAG 
CCAGGAAAGC CAGCCCTTCC TCCTGAAGAG TGTGCCCAGG AGGAGCCTGA GGTCACCACC 
CCAGCCAGCA CCATCAGCAG CTCCACCCTG TCAGTTGGCA GTTTTTCAGA GCACTTGGAT 
CAGATAAATG GACGAAGCGA GTGTGTGGAC AGTACAGATA ATTCCTCAAA GCCATCCAGT 
GAACCCGCTT CTCACATGGC TCGGCAGCGA TTAGAAAGCA CAGAAAAAAA GAAAATCTCG 
GGGAAAGTCA CAAAGTCCCT CTCTGCCAGT GCTCTTTCCC TCATGATCCC AGGAGATATG 
TTTGCTGTTT CCCCTCTGGG AAGTCCAATG TCTCCCCATT CCCTGTCCTC GGACCCTTCT 
TCTTCACGAG ATTCCTCTCC CAGCCGAGAT TCCTCAGCAG CTTCTGCCAG TCCACATCAG 
CCGATTGTGA TCCACAGTTC GGGGAAGAAC TACGGCTTTA CCATCCGAGC CATCCGGGTG 
TATGTGGGAG ACAGTGACAT CTATACAGTG CACCATATCG TCTGGAATGT AGAAGAAGGA 
AGTCCGGCAT GCCAGGCAGG ACTGAAGGCT GGAGATCTTA TCACTCACAT CAATGGAGAA 
CCAGTGCATG GACTTGTCCA CACAGAAGTT ATAGAACTCC TACTGAAGAG TGGGAATAAG 
GTGTCAATCA CTACTACCCC ATTTGAAAAC ACATCAATCA AAACTGGACC AGCCAGGAGA 
AACAGCTATA AGAGCCGGAT GGTGAGGCGG AGCAAGAAAT CCAAGAAGAA AGAAAGTCTC 
GAAAGGAGGA GATCTCTTTT CAAAAAGCTA GCCAAGCAGC CTTCTCCTTT ACTCCACACC 
AGCCGAAGTT TCTCCTGCTT GAACAGATCC CTGTCATCGG GTGAGAGCCT CCCAGGTTCC 
CCCACTCATA GCTTGTCTCC CCGGTCTCCA ACACCAAGCT ACCGCTCCAC CCCTGACTTC 
CCATCTGGTA CTAATTCCTC CCAGAGCAGC TCCCCTAGTT CTAGTGCCCC CAATTCCCCA 
GCAGGGTCCG GGCACATCCG GCCCAGCACT CTCCACGGTC TTGCACCCAA ACTCGGCGGG 
CAGCGGTACC GGTCCGGAAG GCGAAAGTCC GCCGGCAACA TCCCACTGTC CCCGCTGGCC 
CGGACGCCCT CTCCAACCCC GCAACCCACC TCCCCGCAGC GGTCACCATC CCCTCTTCTG 
GGACACTCAC TGGGCAATTC CAAGATCGCG CAAGCCTTTC CCAGCAAGAT GCACTCCCCG 
CCCACCATCG TCAGACACAT CGTGAGGCCC AAGAGTGCGG AGCCCCCCAG GTCCCCGCTG 
CTCAAGCGCG TGCAGTCCGA GGAGAAGCTG TCGCCCTCTT ACGGCAGTGA CAAGAAGCAC 
CTGTGCTCCC GCAAGCACAG CCTGGAGGTG ACCCAAGAGG AGGTGCAGCG GGAGCAGTCC 
CAGCGGGAGG CGCCGCTGCA GAGCCTGGAT GAGAACGTGT GCGACGTGCC GCCGCTCAGC 
CGCGCCCGGC CAGTGGAGCA AGGCTGCCTG AAACGCCCAG TCTCCCGGAA GGTGGGCCGC 
CAGGAGTCTG TGGACGACCT GGACCGCGAC AAGCTGAAGG CCAAGGTGGT GGTGAAGAAA 
GCAGACGGCT TCCCAGAGAA ACAGGAATCC CACCAGAAAT CCCATGGACC CGGGAGTGAT 
TTGGAAAACT TTGCTCTGTT TAAGCTGGAA GAGAGAGAGA AGAAAGTCTA TCCGAAGGCT 
GTGGAAAGGT CAAGTACTTT TGAAAACAAA GCGTCTATGC AGGAGGCGCC ACCGCTGGGC 
AGCCTGCTGA AGGATGCTCT TCACAAGCAG GCCAGCGTGC GCGCCAGCGA GGGTGCGATG 
TCGGATGGCC GGGTGCCTGC GGAGCACCGC CAGGGTGGCG GGGACTTCAG ACGGGCCCCC 
GCTCCTGGCA CCCTCCAGGA TGGTCTCTGC CACTCCCTCG ACAGGGGCAT CTCTGGGAAG 
GGGGAAGGCA CGGAGAAGTC CTCCCAGGCC AAGGAGCTTC TCCGATGTGA AAAGTTAGAC 
AGCAAGCTGG CCAACATCGA TTACCTCCGA AAGAAAATGT CACTTGAGGA CAAAGAGGAC 
AACCTCTGCC CTGTGCTGAA GCCCAAGATG ACAGCTGGCT CCCACGAATG CCTGCCAGGG 
AACCCAGTCC GACCCACGGG TGGGCAGCAG GAGCCCCCGC CGGCTTCTGA GAGCCGAGCT 
TTTGTCAGCA GCACCCATGC AGCTCAGATG AGTGCCGTCT CTTTTGTTCC CCTCAAGGCC 
TTAACAGGCC GGGTGGACAG TGGAACGGAG AAGCCTGGCT TGGTTGCTCC TGAGTCCCCT 
GTTAGGAAGA GCCCCTCCGA GTATAAGCTG GAAGGTAGGT CTGTCTCATG CCTGAAGCCG 
ATCGAGGGCA CTCTGGACAT TGCTCTCCTG TCCGGACCTC AGGCCTCCAA GACAGAACTG 
CCTTCCCCAG AGTCTGCACA GAGCCCCAGC CCAAGTGGTG ACGTGAGGGC CTCTGTGCCA 
CCAGTTCTCC CCAGCAGCAG TGGGAAAAAG AACGATACCA CCAGTGCAAG AGAGCTTTCT 
CCTTCCAGCT TAAAGATGAA TAAATCCTAC CTGCTGGAGC CTTGGTTCCT GCCCCCCAGC 
CGAGGTCTCC AGAATTCACC AGCAGTTTCC CTGCCTGACC CAGAGTTCAA GAGGGACAGG 
AAAGGTCCCC ATCCTACTGC CAGGAGCCCT GGAACAGTCA TGGAAAGCAA TCCCCAACAG 
AGAGAGGGCA GCTCCCCTAA ACACCAAGAC CACACCACTG ACCCCAAGCT TCTGACCTGC 
CTGGGGCAGA ACCTCCACAG CCCTGACCTG GCCAGGCCAC GCTGCCCGCT CCCACCTGAA 
GCTTCCCCCT CAAGGGAGAA GCCAGGCCTG AGGGAATCGT CTGAAAGAGG CCCTCCCACA 
GCCAGAAGCG AGCGCTCTGC TGCGAGGGCT GACACATGCA GAGAGCCCTC CATGGAACTG 
TGCTTTCCAG AAACTGCGAA AACCAGTGAC AACTCCAAAA ATCTCCTCTC TGTGGGAAGG 
ACCCACCCAG ATTTCTATAC ACAGACCCAG GCCATGGAGA AAGCATGGGC GCCGGGTGGG 
AAAACGAACC ACAAAGATGG CCCAGGTGAG GCGAGGCCCC CGCCCAGAGA CAACTCCTCT 
CTGCACTCAG CTGGAATTCC CTGTGAGAAG GAGCTGGGCA AGGTGAGGCG TGGCGTGGAA 
CCCAAGCCCG AAGCGCTTCT TGCCAGGCGG TCTCTGCAGC CACCTGGAAT TGAGAGTGAG 
AAGAGTGAAA AGCTCTCCAG TTTCCCATCT TTGCAGAAAG ATGGTGCCAA GGAACCTGAA 
AGGAAGGAGC AGCCTCTACA AAGGCATCCC AGCAGCATCC CTCCGCCCCC TCTGACGGCC 
AAAGACCTGT CCAGCCCGGC TGCCAGGCAG CATTGCAGTT CCCCAAGCCA CGCTTCTGGC 
AGAGAGCCGG GGGCCAAGCC CAGCACTGCA GAGCCCAGCT CGAGCCCCCA GGACCCTCCC 
AAGCCTGTTG CTGCGCACAG TGAAAGCAGC AGCCACAAGC CCCGGCCTGG CCCTGACCCG 
GGCCCTCCAA AGACTAAGCA CCCCGACCGG TCCCTCTCCT CTCAGAAACC AAGTGTCGGG 
GCCACAAAGG GCAAAGAGCC TGCCACTCAG TCCCTCGGTG GCTCTAGCAG AGAGGGGAAG 
GGCCACAGTA AGAGTGGGCC GGATGTGTTT CCTGCTACCC CAGGCTCCCA GAACAAAGCC 
AGCGATGGGA TTGGCCAGGG AGAAGGTGGG CCCTCTGTCC CACTGCACAC TGACAGGGCT 
CCTCTAGACG CCAAGCCACA ACCCACCAGT GGTGGGCGGC CCCTGGAGGT GCTGGAGAAG 
CCTGTGCATT TGCCAAGGCC GGGACACCCA GGGCCTAGTG AGCCAGCGGA CCAGAAACTG 
TCCGCTGTTG GTGAAAAGCA AACCCTGTCT CCAAAGCACC CCAAACCATC CACTGTGAAA 
GATTGCCCCA CCCTGTGCAA ACAGACAGAC AACAGACAGA CAGACAAAAG CCCGAGTCAG 
CCGGCCGCCA ACACCGACAG AAGGGCGGAA GGGAAGAAAT GCACTGAAGC ACTTTATGCT 
CCAGCAGAGG GCGACAAGCT CGAGGCCGGC CTTTCCTTTG TGCATAGCGA GAACCGGTTG 
AAAGGCGCGG AGCGGCCAGC CGCGGGGGTG GGGAAGGGCT TCCCTGAGGC CAGAGGGAAA 
GGGCCCGGTC CCCAGAAGCC ACCGACGGAG GCAGACAAGC CCAATGGCAT GAAACGGTCC 
CCCTCAGCCA CTGGGCAGAG TTCTTTCCGA TCCACGGCCC TCCCGGAAAA GTCTCTGAGC 
TGCTCCTCCA GCTTCCCTGA AACCAGGGCC GGAGTTAGAG AGGCCTCTGC AGCCAGCAGC 
GACACCTCTT CTGCCAAGGC CGCCGGGGGC ATGCTGGAGC TTCCAGCCCC CAGCAACAGG 
GACCATAGGA AGGCTCAGCC TGCCGGGGAG GGCCGAACCC ACATGACAAA GAGTGACTCC 
CTGCCCTCCT TCCGGGTCTC CACCCTGCCT CTGGAGTCAC ACCACCCCGA CCCAAACACC 
ATGGGCGGGG CCAGCCACCG GGACAGGGCT CTCTCGGTGA CTGCCACCGT AGGGGAAACC 
AAAGGGAAGG ACCCTGCCCC AGCCCAGCCT CCCCCAGCTA GGAAACAGAA CGTGGGCAGA 
GACGTGACCA AGCCATCCCC AGCCCCAAAC ACTGACCGCC CCATCTCTCT TTCTAATGAG 
AAGGACTTTG TGGTACGGCA GAGGCGGGGG AAAGAGAGTT TGCGTAGCAG CCCTCACAAA 
AAGGCCTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541686.1
CDS	2060..9331
Translation	MCIPLQNQIIPRDRLLYKAQQATKSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_011543384.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543384.2

ORF Insert Sequence:

ATGTGTATAC CTTTACAGAA TCAAATAATT CCCAGGGATC GTCTGCTATA CAAAGCCCAA 
CAAGCCACTA AAAGTTGCCG AACAAGCAAC CGGAAAAGCT TAATAGGCAA TGGGCAGTCA 
CCAGCATTGC CTCGACCACA CTCACCTCTC TCTGCTCATG CAGGAAATAG CCCTCAAGAT 
AGTCCAAGAA ATTTCTCCCC CAGTGCCTCA GCCCATTTTT CATTTGCACG GAGGACTGAT 
GGACGCCGCT GGTCGTTGGC TTCTCTCCCT TCCTCTGGCT ATGGGACAAA CACACCCAGC 
TCTACGGTCT CTTCATCCTG TTCCTCCCAG GAGAAGTTGC ATCAGTTACC ATACCAACCA 
ACACCAGACG AGTTACACTT CTTATCAAAA CATTTCTGTA CCACCGAAAG CATCGCCACT 
GAGAACAGAT GCAGGAACAC GCCGATGCGC CCCCGTTCCC GAAGTCTGAG CCCTGGACGT 
TCTCCCGCCT GCTGTGACCA TGAAATAATT ATGATGAACC ATGTCTACAA AGAAAGGTTC 
CCAAAGGCTA CAGCTCAGAT GGAAGAACGT CTAAAGGAAA TTATCACCAG CTACTCTCCT 
GACAACGTTC TACCCTTAGC AGATGGAGTG CTTAGTTTCA CTCACCACCA GATTATTGAA 
CTGGCTCGAG ATTGCTTGGA TAAATCCCAC CAGGGCCTCA TCACCTCACG ATACTTCCTT 
GAATTACAGC ACAAATTAGA TAAGTTGCTA CAGGAGGCTC ATGATCGTTC AGAAAGTGGA 
GAATTGGCAT TTATTAAACA ACTAGTTCGA AAGATCCTAA TTGTTATTGC CCGCCCTGCT 
CGGTTATTAG AGTGCCTGGA ATTTGATCCG GAAGAATTTT ACTACCTATT GGAAGCAGCA 
GAAGGCCATG CCAAAGAAGG ACAGGGTATT AAAACCGACA TTCCCAGGTA CATCATTAGC 
CAACTGGGAC TCAATAAGGA TCCCTTGGAA GAAATGGCTC ATTTGGGAAA CTACGATAGT 
GGGACAGCAG AAACACCAGA AACAGATGAA TCAGTGAGTA GCTCTAATGC CTCCCTGAAA 
CTTCGAAGGA AACCTCGGGA AAGTGATTTT GAAACGATTA AATTGATTAG CAATGGAGCC 
TATGGGGCAG TCTACTTTGT TCGGCATAAA GAATCCCGGC AGAGGTTTGC CATGAAGAAG 
ATTAATAAAC AGAACCTCAT CCTTCGAAAC CAGATCCAGC AGGCCTTTGT GGAGCGGGAT 
ATCCTGACTT TTGCAGAAAA CCCCTTTGTT GTCAGCATGT ATTGCTCCTT TGAAACAAGG 
CGCCACTTGT GCATGGTCAT GGAATATGTG GAAGGGGGAG ACTGTGCTAC TTTAATGAAA 
AACATGGGTC CTCTCCCTGT TGATATGGCC AGAATGTACT TTGCTGAGAC GGTCTTGGCC 
TTGGAATATT TACATAATTA TGGAATTGTA CACAGGGATT TGAAACCAGA CAACTTGTTG 
GTTACCTCCA TGGGGCACAT AAAGCTGACA GATTTTGGAT TATCTAAGGT GGGACTAATG 
AGCATGACTA CCAACCTTTA CGAGGGTCAT ATTGAGAAGG ATGCTAGAGA GTTCCTGGAT 
AAACAGGTCT GTGGCACACC TGAATACATT GCACCAGAAG TGATTCTGAG GCAGGGTTAT 
GGAAAGCCGG TGGACTGGTG GGCCATGGGG ATTATCCTCT ATGAATTTCT GGTTGGATGC 
GTGCCATTCT TTGGGGATAC TCCAGAGGAG CTATTTGGAC AAGTCATCAG TGATGAGATC 
AACTGGCCTG AGAAGGATGA GGCACCCCCA CCTGATGCCC AGGATCTGAT TACCTTACTC 
CTCAGGCAGA ATCCCCTGGA GAGGCTGGGA ACAGGTGGTG CATATGAAGT CAAACAGCAT 
CGATTCTTCC GTTCTTTAGA CTGGAACAGT TTGCTGAGAC AGAAGGCAGA ATTTATTCCC 
CAACTGGAAT CTGAGGATGA CACAAGTTAT TTTGATACTC GGTCTGAGAA GTATCATCAT 
ATGGAAACGG AGGAAGAAGA TGACACAAAT GATGAAGACT TTAATGTGGA AATAAGGCAG 
TTTTCTTCAT GTTCACACAG GTTTTCAAAA GTTTTCAGCA GTATAGATCG AATCACTCAG 
AATTCAGCAG AAGAGAAGGA AGACTCTGTG GACAAAACCA AAAGCACCAC CTTGCCATCC 
ACAGAAACAC TGAGCTGGAG TTCAGAATAT TCTGAAATGC AACAGCTATC AACATCCAAC 
TCTTCAGATA CTGAAAGCAA CAGACATAAA CTCAGTTCTG GCCTACTTCC CAAACTGGCT 
ATTTCAACAG AGGGAGAGCA AGATGAAGCT GCCTCCTGCC CTGGAGACCC CCATGAGGAG 
CCAGGAAAGC CAGCCCTTCC TCCTGAAGAG TGTGCCCAGG AGGAGCCTGA GGTCACCACC 
CCAGCCAGCA CCATCAGCAG CTCCACCCTG TCAGTTGGCA GTTTTTCAGA GCACTTGGAT 
CAGATAAATG GACGAAGCGA GTGTGTGGAC AGTACAGATA ATTCCTCAAA GCCATCCAGT 
GAACCCGCTT CTCACATGGC TCGGCAGCGA TTAGAAAGCA CAGAAAAAAA GAAAATCTCG 
GGGAAAGTCA CAAAGTCCCT CTCTGCCAGT GCTCTTTCCC TCATGATCCC AGGAGATATG 
TTTGCTGTTT CCCCTCTGGG AAGTCCAATG TCTCCCCATT CCCTGTCCTC GGACCCTTCT 
TCTTCACGAG ATTCCTCTCC CAGCCGAGAT TCCTCAGCAG CTTCTGCCAG TCCACATCAG 
CCGATTGTGA TCCACAGTTC GGGGAAGAAC TACGGCTTTA CCATCCGAGC CATCCGGGTG 
TATGTGGGAG ACAGTGACAT CTATACAGTG CACCATATCG TCTGGAATGT AGAAGAAGGA 
AGTCCGGCAT GCCAGGCAGG ACTGAAGGCT GGAGATCTTA TCACTCACAT CAATGGAGAA 
CCAGTGCATG GACTTGTCCA CACAGAAGTT ATAGAACTCC TACTGAAGAG TGGGAATAAG 
GTGTCAATCA CTACTACCCC ATTTGAAAAC ACATCAATCA AAACTGGACC AGCCAGGAGA 
AACAGCTATA AGAGCCGGAT GGTGAGGCGG AGCAAGAAAT CCAAGAAGAA AGAAAGTCTC 
GAAAGGAGGA GATCTCTTTT CAAAAAGCTA GCCAAGCAGC CTTCTCCTTT ACTCCACACC 
AGCCGAAGTT TCTCCTGCTT GAACAGATCC CTGTCATCGG GTGAGAGCCT CCCAGGTTCC 
CCCACTCATA GCTTGTCTCC CCGGTCTCCA ACACCAAGCT ACCGCTCCAC CCCTGACTTC 
CCATCTGGTA CTAATTCCTC CCAGAGCAGC TCCCCTAGTT CTAGTGCCCC CAATTCCCCA 
GCAGGGTCCG GGCACATCCG GCCCAGCACT CTCCACGGTC TTGCACCCAA ACTCGGCGGG 
CAGCGGTACC GGTCCGGAAG GCGAAAGTCC GCCGGCAACA TCCCACTGTC CCCGCTGGCC 
CGGACGCCCT CTCCAACCCC GCAACCCACC TCCCCGCAGC GGTCACCATC CCCTCTTCTG 
GGACACTCAC TGGGCAATTC CAAGATCGCG CAAGCCTTTC CCAGCAAGAT GCACTCCCCG 
CCCACCATCG TCAGACACAT CGTGAGGCCC AAGAGTGCGG AGCCCCCCAG GTCCCCGCTG 
CTCAAGCGCG TGCAGTCCGA GGAGAAGCTG TCGCCCTCTT ACGGCAGTGA CAAGAAGCAC 
CTGTGCTCCC GCAAGCACAG CCTGGAGGTG ACCCAAGAGG AGGTGCAGCG GGAGCAGTCC 
CAGCGGGAGG CGCCGCTGCA GAGCCTGGAT GAGAACGTGT GCGACGTGCC GCCGCTCAGC 
CGCGCCCGGC CAGTGGAGCA AGGCTGCCTG AAACGCCCAG TCTCCCGGAA GGTGGGCCGC 
CAGGAGTCTG TGGACGACCT GGACCGCGAC AAGCTGAAGG CCAAGGTGGT GGTGAAGAAA 
GCAGACGGCT TCCCAGAGAA ACAGGAATCC CACCAGAAAT CCCATGGACC CGGGAGTGAT 
TTGGAAAACT TTGCTCTGTT TAAGCTGGAA GAGAGAGAGA AGAAAGTCTA TCCGAAGGCT 
GTGGAAAGGT CAAGTACTTT TGAAAACAAA GCGTCTATGC AGGAGGCGCC ACCGCTGGGC 
AGCCTGCTGA AGGATGCTCT TCACAAGCAG GCCAGCGTGC GCGCCAGCGA GGGTGCGATG 
TCGGATGGCC GGGTGCCTGC GGAGCACCGC CAGGGTGGCG GGGACTTCAG ACGGGCCCCC 
GCTCCTGGCA CCCTCCAGGA TGGTCTCTGC CACTCCCTCG ACAGGGGCAT CTCTGGGAAG 
GGGGAAGGCA CGGAGAAGTC CTCCCAGGCC AAGGAGCTTC TCCGATGTGA AAAGTTAGAC 
AGCAAGCTGG CCAACATCGA TTACCTCCGA AAGAAAATGT CACTTGAGGA CAAAGAGGAC 
AACCTCTGCC CTGTGCTGAA GCCCAAGATG ACAGCTGGCT CCCACGAATG CCTGCCAGGG 
AACCCAGTCC GACCCACGGG TGGGCAGCAG GAGCCCCCGC CGGCTTCTGA GAGCCGAGCT 
TTTGTCAGCA GCACCCATGC AGCTCAGATG AGTGCCGTCT CTTTTGTTCC CCTCAAGGCC 
TTAACAGGCC GGGTGGACAG TGGAACGGAG AAGCCTGGCT TGGTTGCTCC TGAGTCCCCT 
GTTAGGAAGA GCCCCTCCGA GTATAAGCTG GAAGGTAGGT CTGTCTCATG CCTGAAGCCG 
ATCGAGGGCA CTCTGGACAT TGCTCTCCTG TCCGGACCTC AGGCCTCCAA GACAGAACTG 
CCTTCCCCAG AGTCTGCACA GAGCCCCAGC CCAAGTGGTG ACGTGAGGGC CTCTGTGCCA 
CCAGTTCTCC CCAGCAGCAG TGGGAAAAAG AACGATACCA CCAGTGCAAG AGAGCTTTCT 
CCTTCCAGCT TAAAGATGAA TAAATCCTAC CTGCTGGAGC CTTGGTTCCT GCCCCCCAGC 
CGAGGTCTCC AGAATTCACC AGCAGTTTCC CTGCCTGACC CAGAGTTCAA GAGGGACAGG 
AAAGGTCCCC ATCCTACTGC CAGGAGCCCT GGAACAGTCA TGGAAAGCAA TCCCCAACAG 
AGAGAGGGCA GCTCCCCTAA ACACCAAGAC CACACCACTG ACCCCAAGCT TCTGACCTGC 
CTGGGGCAGA ACCTCCACAG CCCTGACCTG GCCAGGCCAC GCTGCCCGCT CCCACCTGAA 
GCTTCCCCCT CAAGGGAGAA GCCAGGCCTG AGGGAATCGT CTGAAAGAGG CCCTCCCACA 
GCCAGAAGCG AGCGCTCTGC TGCGAGGGCT GACACATGCA GAGAGCCCTC CATGGAACTG 
TGCTTTCCAG AAACTGCGAA AACCAGTGAC AACTCCAAAA ATCTCCTCTC TGTGGGAAGG 
ACCCACCCAG ATTTCTATAC ACAGACCCAG GCCATGGAGA AAGCATGGGC GCCGGGTGGG 
AAAACGAACC ACAAAGATGG CCCAGGTGAG GCGAGGCCCC CGCCCAGAGA CAACTCCTCT 
CTGCACTCAG CTGGAATTCC CTGTGAGAAG GAGCTGGGCA AGGTGAGGCG TGGCGTGGAA 
CCCAAGCCCG AAGCGCTTCT TGCCAGGCGG TCTCTGCAGC CACCTGGAAT TGAGAGTGAG 
AAGAGTGAAA AGCTCTCCAG TTTCCCATCT TTGCAGAAAG ATGGTGCCAA GGAACCTGAA 
AGGAAGGAGC AGCCTCTACA AAGGCATCCC AGCAGCATCC CTCCGCCCCC TCTGACGGCC 
AAAGACCTGT CCAGCCCGGC TGCCAGGCAG CATTGCAGTT CCCCAAGCCA CGCTTCTGGC 
AGAGAGCCGG GGGCCAAGCC CAGCACTGCA GAGCCCAGCT CGAGCCCCCA GGACCCTCCC 
AAGCCTGTTG CTGCGCACAG TGAAAGCAGC AGCCACAAGC CCCGGCCTGG CCCTGACCCG 
GGCCCTCCAA AGACTAAGCA CCCCGACCGG TCCCTCTCCT CTCAGAAACC AAGTGTCGGG 
GCCACAAAGG GCAAAGAGCC TGCCACTCAG TCCCTCGGTG GCTCTAGCAG AGAGGGGAAG 
GGCCACAGTA AGAGTGGGCC GGATGTGTTT CCTGCTACCC CAGGCTCCCA GAACAAAGCC 
AGCGATGGGA TTGGCCAGGG AGAAGGTGGG CCCTCTGTCC CACTGCACAC TGACAGGGCT 
CCTCTAGACG CCAAGCCACA ACCCACCAGT GGTGGGCGGC CCCTGGAGGT GCTGGAGAAG 
CCTGTGCATT TGCCAAGGCC GGGACACCCA GGGCCTAGTG AGCCAGCGGA CCAGAAACTG 
TCCGCTGTTG GTGAAAAGCA AACCCTGTCT CCAAAGCACC CCAAACCATC CACTGTGAAA 
GATTGCCCCA CCCTGTGCAA ACAGACAGAC AACAGACAGA CAGACAAAAG CCCGAGTCAG 
CCGGCCGCCA ACACCGACAG AAGGGCGGAA GGGAAGAAAT GCACTGAAGC ACTTTATGCT 
CCAGCAGAGG GCGACAAGCT CGAGGCCGGC CTTTCCTTTG TGCATAGCGA GAACCGGTTG 
AAAGGCGCGG AGCGGCCAGC CGCGGGGGTG GGGAAGGGCT TCCCTGAGGC CAGAGGGAAA 
GGGCCCGGTC CCCAGAAGCC ACCGACGGAG GCAGACAAGC CCAATGGCAT GAAACGGTCC 
CCCTCAGCCA CTGGGCAGAG TTCTTTCCGA TCCACGGCCC TCCCGGAAAA GTCTCTGAGC 
TGCTCCTCCA GCTTCCCTGA AACCAGGGCC GGAGTTAGAG AGGCCTCTGC AGCCAGCAGC 
GACACCTCTT CTGCCAAGGC CGCCGGGGGC ATGCTGGAGC TTCCAGCCCC CAGCAACAGG 
GACCATAGGA AGGCTCAGCC TGCCGGGGAG GGCCGAACCC ACATGACAAA GAGTGACTCC 
CTGCCCTCCT TCCGGGTCTC CACCCTGCCT CTGGAGTCAC ACCACCCCGA CCCAAACACC 
ATGGGCGGGG CCAGCCACCG GGACAGGGCT CTCTCGGTGA CTGCCACCGT AGGGGAAACC 
AAAGGGAAGG ACCCTGCCCC AGCCCAGCCT CCCCCAGCTA GGAAACAGAA CGTGGGCAGA 
GACGTGACCA AGCCATCCCC AGCCCCAAAC ACTGACCGCC CCATCTCTCT TTCTAATGAG 
AAGGACTTTG TGGTACGGCA GAGGCGGGGG AAAGAGAGTT TGCGTAGCAG CCCTCACAAA 
AAGGCCTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_011543385.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541687.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA.

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CloneID	OHu79756
Clone ID Related Accession (Same CDS sequence)	XM_011543385.2 , XM_011543385.3
Accession Version	XM_011543385.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543385.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541687.1
CDS	3207..10451
Translation	MKAQRERLQIPGLTLDCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_011543385.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543385.2

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_011543385.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541687.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X6, mRNA.

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CloneID	OHu79756
Clone ID Related Accession (Same CDS sequence)	XM_011543385.2 , XM_011543385.3
Accession Version	XM_011543385.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011543385.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541687.1
CDS	3208..10452
Translation	MKAQRERLQIPGLTLDCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_011543385.3
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543385.3

ORF Insert Sequence:

ATGAAAGCCC AGCGGGAAAG GCTACAGATT CCGGGGCTGA CCTTGGATTG CCGAACAAGC 
AACCGGAAAA GCTTAATAGG CAATGGGCAG TCACCAGCAT TGCCTCGACC ACACTCACCT 
CTCTCTGCTC ATGCAGGAAA TAGCCCTCAA GATAGTCCAA GAAATTTCTC CCCCAGTGCC 
TCAGCCCATT TTTCATTTGC ACGGAGGACT GATGGACGCC GCTGGTCGTT GGCTTCTCTC 
CCTTCCTCTG GCTATGGGAC AAACACACCC AGCTCTACGG TCTCTTCATC CTGTTCCTCC 
CAGGAGAAGT TGCATCAGTT ACCATACCAA CCAACACCAG ACGAGTTACA CTTCTTATCA 
AAACATTTCT GTACCACCGA AAGCATCGCC ACTGAGAACA GATGCAGGAA CACGCCGATG 
CGCCCCCGTT CCCGAAGTCT GAGCCCTGGA CGTTCTCCCG CCTGCTGTGA CCATGAAATA 
ATTATGATGA ACCATGTCTA CAAAGAAAGG TTCCCAAAGG CTACAGCTCA GATGGAAGAA 
CGTCTAAAGG AAATTATCAC CAGCTACTCT CCTGACAACG TTCTACCCTT AGCAGATGGA 
GTGCTTAGTT TCACTCACCA CCAGATTATT GAACTGGCTC GAGATTGCTT GGATAAATCC 
CACCAGGGCC TCATCACCTC ACGATACTTC CTTGAATTAC AGCACAAATT AGATAAGTTG 
CTACAGGAGG CTCATGATCG TTCAGAAAGT GGAGAATTGG CATTTATTAA ACAACTAGTT 
CGAAAGATCC TAATTGTTAT TGCCCGCCCT GCTCGGTTAT TAGAGTGCCT GGAATTTGAT 
CCGGAAGAAT TTTACTACCT ATTGGAAGCA GCAGAAGGCC ATGCCAAAGA AGGACAGGGT 
ATTAAAACCG ACATTCCCAG GTACATCATT AGCCAACTGG GACTCAATAA GGATCCCTTG 
GAAGAAATGG CTCATTTGGG AAACTACGAT AGTGGGACAG CAGAAACACC AGAAACAGAT 
GAATCAGTGA GTAGCTCTAA TGCCTCCCTG AAACTTCGAA GGAAACCTCG GGAAAGTGAT 
TTTGAAACGA TTAAATTGAT TAGCAATGGA GCCTATGGGG CAGTCTACTT TGTTCGGCAT 
AAAGAATCCC GGCAGAGGTT TGCCATGAAG AAGATTAATA AACAGAACCT CATCCTTCGA 
AACCAGATCC AGCAGGCCTT TGTGGAGCGG GATATCCTGA CTTTTGCAGA AAACCCCTTT 
GTTGTCAGCA TGTATTGCTC CTTTGAAACA AGGCGCCACT TGTGCATGGT CATGGAATAT 
GTGGAAGGGG GAGACTGTGC TACTTTAATG AAAAACATGG GTCCTCTCCC TGTTGATATG 
GCCAGAATGT ACTTTGCTGA GACGGTCTTG GCCTTGGAAT ATTTACATAA TTATGGAATT 
GTACACAGGG ATTTGAAACC AGACAACTTG TTGGTTACCT CCATGGGGCA CATAAAGCTG 
ACAGATTTTG GATTATCTAA GGTGGGACTA ATGAGCATGA CTACCAACCT TTACGAGGGT 
CATATTGAGA AGGATGCTAG AGAGTTCCTG GATAAACAGG TCTGTGGCAC ACCTGAATAC 
ATTGCACCAG AAGTGATTCT GAGGCAGGGT TATGGAAAGC CGGTGGACTG GTGGGCCATG 
GGGATTATCC TCTATGAATT TCTGGTTGGA TGCGTGCCAT TCTTTGGGGA TACTCCAGAG 
GAGCTATTTG GACAAGTCAT CAGTGATGAG ATCAACTGGC CTGAGAAGGA TGAGGCACCC 
CCACCTGATG CCCAGGATCT GATTACCTTA CTCCTCAGGC AGAATCCCCT GGAGAGGCTG 
GGAACAGGTG GTGCATATGA AGTCAAACAG CATCGATTCT TCCGTTCTTT AGACTGGAAC 
AGTTTGCTGA GACAGAAGGC AGAATTTATT CCCCAACTGG AATCTGAGGA TGACACAAGT 
TATTTTGATA CTCGGTCTGA GAAGTATCAT CATATGGAAA CGGAGGAAGA AGATGACACA 
AATGATGAAG ACTTTAATGT GGAAATAAGG CAGTTTTCTT CATGTTCACA CAGGTTTTCA 
AAAGTTTTCA GCAGTATAGA TCGAATCACT CAGAATTCAG CAGAAGAGAA GGAAGACTCT 
GTGGACAAAA CCAAAAGCAC CACCTTGCCA TCCACAGAAA CACTGAGCTG GAGTTCAGAA 
TATTCTGAAA TGCAACAGCT ATCAACATCC AACTCTTCAG ATACTGAAAG CAACAGACAT 
AAACTCAGTT CTGGCCTACT TCCCAAACTG GCTATTTCAA CAGAGGGAGA GCAAGATGAA 
GCTGCCTCCT GCCCTGGAGA CCCCCATGAG GAGCCAGGAA AGCCAGCCCT TCCTCCTGAA 
GAGTGTGCCC AGGAGGAGCC TGAGGTCACC ACCCCAGCCA GCACCATCAG CAGCTCCACC 
CTGTCAGTTG GCAGTTTTTC AGAGCACTTG GATCAGATAA ATGGACGAAG CGAGTGTGTG 
GACAGTACAG ATAATTCCTC AAAGCCATCC AGTGAACCCG CTTCTCACAT GGCTCGGCAG 
CGATTAGAAA GCACAGAAAA AAAGAAAATC TCGGGGAAAG TCACAAAGTC CCTCTCTGCC 
AGTGCTCTTT CCCTCATGAT CCCAGGAGAT ATGTTTGCTG TTTCCCCTCT GGGAAGTCCA 
ATGTCTCCCC ATTCCCTGTC CTCGGACCCT TCTTCTTCAC GAGATTCCTC TCCCAGCCGA 
GATTCCTCAG CAGCTTCTGC CAGTCCACAT CAGCCGATTG TGATCCACAG TTCGGGGAAG 
AACTACGGCT TTACCATCCG AGCCATCCGG GTGTATGTGG GAGACAGTGA CATCTATACA 
GTGCACCATA TCGTCTGGAA TGTAGAAGAA GGAAGTCCGG CATGCCAGGC AGGACTGAAG 
GCTGGAGATC TTATCACTCA CATCAATGGA GAACCAGTGC ATGGACTTGT CCACACAGAA 
GTTATAGAAC TCCTACTGAA GAGTGGGAAT AAGGTGTCAA TCACTACTAC CCCATTTGAA 
AACACATCAA TCAAAACTGG ACCAGCCAGG AGAAACAGCT ATAAGAGCCG GATGGTGAGG 
CGGAGCAAGA AATCCAAGAA GAAAGAAAGT CTCGAAAGGA GGAGATCTCT TTTCAAAAAG 
CTAGCCAAGC AGCCTTCTCC TTTACTCCAC ACCAGCCGAA GTTTCTCCTG CTTGAACAGA 
TCCCTGTCAT CGGGTGAGAG CCTCCCAGGT TCCCCCACTC ATAGCTTGTC TCCCCGGTCT 
CCAACACCAA GCTACCGCTC CACCCCTGAC TTCCCATCTG GTACTAATTC CTCCCAGAGC 
AGCTCCCCTA GTTCTAGTGC CCCCAATTCC CCAGCAGGGT CCGGGCACAT CCGGCCCAGC 
ACTCTCCACG GTCTTGCACC CAAACTCGGC GGGCAGCGGT ACCGGTCCGG AAGGCGAAAG 
TCCGCCGGCA ACATCCCACT GTCCCCGCTG GCCCGGACGC CCTCTCCAAC CCCGCAACCC 
ACCTCCCCGC AGCGGTCACC ATCCCCTCTT CTGGGACACT CACTGGGCAA TTCCAAGATC 
GCGCAAGCCT TTCCCAGCAA GATGCACTCC CCGCCCACCA TCGTCAGACA CATCGTGAGG 
CCCAAGAGTG CGGAGCCCCC CAGGTCCCCG CTGCTCAAGC GCGTGCAGTC CGAGGAGAAG 
CTGTCGCCCT CTTACGGCAG TGACAAGAAG CACCTGTGCT CCCGCAAGCA CAGCCTGGAG 
GTGACCCAAG AGGAGGTGCA GCGGGAGCAG TCCCAGCGGG AGGCGCCGCT GCAGAGCCTG 
GATGAGAACG TGTGCGACGT GCCGCCGCTC AGCCGCGCCC GGCCAGTGGA GCAAGGCTGC 
CTGAAACGCC CAGTCTCCCG GAAGGTGGGC CGCCAGGAGT CTGTGGACGA CCTGGACCGC 
GACAAGCTGA AGGCCAAGGT GGTGGTGAAG AAAGCAGACG GCTTCCCAGA GAAACAGGAA 
TCCCACCAGA AATCCCATGG ACCCGGGAGT GATTTGGAAA ACTTTGCTCT GTTTAAGCTG 
GAAGAGAGAG AGAAGAAAGT CTATCCGAAG GCTGTGGAAA GGTCAAGTAC TTTTGAAAAC 
AAAGCGTCTA TGCAGGAGGC GCCACCGCTG GGCAGCCTGC TGAAGGATGC TCTTCACAAG 
CAGGCCAGCG TGCGCGCCAG CGAGGGTGCG ATGTCGGATG GCCGGGTGCC TGCGGAGCAC 
CGCCAGGGTG GCGGGGACTT CAGACGGGCC CCCGCTCCTG GCACCCTCCA GGATGGTCTC 
TGCCACTCCC TCGACAGGGG CATCTCTGGG AAGGGGGAAG GCACGGAGAA GTCCTCCCAG 
GCCAAGGAGC TTCTCCGATG TGAAAAGTTA GACAGCAAGC TGGCCAACAT CGATTACCTC 
CGAAAGAAAA TGTCACTTGA GGACAAAGAG GACAACCTCT GCCCTGTGCT GAAGCCCAAG 
ATGACAGCTG GCTCCCACGA ATGCCTGCCA GGGAACCCAG TCCGACCCAC GGGTGGGCAG 
CAGGAGCCCC CGCCGGCTTC TGAGAGCCGA GCTTTTGTCA GCAGCACCCA TGCAGCTCAG 
ATGAGTGCCG TCTCTTTTGT TCCCCTCAAG GCCTTAACAG GCCGGGTGGA CAGTGGAACG 
GAGAAGCCTG GCTTGGTTGC TCCTGAGTCC CCTGTTAGGA AGAGCCCCTC CGAGTATAAG 
CTGGAAGGTA GGTCTGTCTC ATGCCTGAAG CCGATCGAGG GCACTCTGGA CATTGCTCTC 
CTGTCCGGAC CTCAGGCCTC CAAGACAGAA CTGCCTTCCC CAGAGTCTGC ACAGAGCCCC 
AGCCCAAGTG GTGACGTGAG GGCCTCTGTG CCACCAGTTC TCCCCAGCAG CAGTGGGAAA 
AAGAACGATA CCACCAGTGC AAGAGAGCTT TCTCCTTCCA GCTTAAAGAT GAATAAATCC 
TACCTGCTGG AGCCTTGGTT CCTGCCCCCC AGCCGAGGTC TCCAGAATTC ACCAGCAGTT 
TCCCTGCCTG ACCCAGAGTT CAAGAGGGAC AGGAAAGGTC CCCATCCTAC TGCCAGGAGC 
CCTGGAACAG TCATGGAAAG CAATCCCCAA CAGAGAGAGG GCAGCTCCCC TAAACACCAA 
GACCACACCA CTGACCCCAA GCTTCTGACC TGCCTGGGGC AGAACCTCCA CAGCCCTGAC 
CTGGCCAGGC CACGCTGCCC GCTCCCACCT GAAGCTTCCC CCTCAAGGGA GAAGCCAGGC 
CTGAGGGAAT CGTCTGAAAG AGGCCCTCCC ACAGCCAGAA GCGAGCGCTC TGCTGCGAGG 
GCTGACACAT GCAGAGAGCC CTCCATGGAA CTGTGCTTTC CAGAAACTGC GAAAACCAGT 
GACAACTCCA AAAATCTCCT CTCTGTGGGA AGGACCCACC CAGATTTCTA TACACAGACC 
CAGGCCATGG AGAAAGCATG GGCGCCGGGT GGGAAAACGA ACCACAAAGA TGGCCCAGGT 
GAGGCGAGGC CCCCGCCCAG AGACAACTCC TCTCTGCACT CAGCTGGAAT TCCCTGTGAG 
AAGGAGCTGG GCAAGGTGAG GCGTGGCGTG GAACCCAAGC CCGAAGCGCT TCTTGCCAGG 
CGGTCTCTGC AGCCACCTGG AATTGAGAGT GAGAAGAGTG AAAAGCTCTC CAGTTTCCCA 
TCTTTGCAGA AAGATGGTGC CAAGGAACCT GAAAGGAAGG AGCAGCCTCT ACAAAGGCAT 
CCCAGCAGCA TCCCTCCGCC CCCTCTGACG GCCAAAGACC TGTCCAGCCC GGCTGCCAGG 
CAGCATTGCA GTTCCCCAAG CCACGCTTCT GGCAGAGAGC CGGGGGCCAA GCCCAGCACT 
GCAGAGCCCA GCTCGAGCCC CCAGGACCCT CCCAAGCCTG TTGCTGCGCA CAGTGAAAGC 
AGCAGCCACA AGCCCCGGCC TGGCCCTGAC CCGGGCCCTC CAAAGACTAA GCACCCCGAC 
CGGTCCCTCT CCTCTCAGAA ACCAAGTGTC GGGGCCACAA AGGGCAAAGA GCCTGCCACT 
CAGTCCCTCG GTGGCTCTAG CAGAGAGGGG AAGGGCCACA GTAAGAGTGG GCCGGATGTG 
TTTCCTGCTA CCCCAGGCTC CCAGAACAAA GCCAGCGATG GGATTGGCCA GGGAGAAGGT 
GGGCCCTCTG TCCCACTGCA CACTGACAGG GCTCCTCTAG ACGCCAAGCC ACAACCCACC 
AGTGGTGGGC GGCCCCTGGA GGTGCTGGAG AAGCCTGTGC ATTTGCCAAG GCCGGGACAC 
CCAGGGCCTA GTGAGCCAGC GGACCAGAAA CTGTCCGCTG TTGGTGAAAA GCAAACCCTG 
TCTCCAAAGC ACCCCAAACC ATCCACTGTG AAAGATTGCC CCACCCTGTG CAAACAGACA 
GACAACAGAC AGACAGACAA AAGCCCGAGT CAGCCGGCCG CCAACACCGA CAGAAGGGCG 
GAAGGGAAGA AATGCACTGA AGCACTTTAT GCTCCAGCAG AGGGCGACAA GCTCGAGGCC 
GGCCTTTCCT TTGTGCATAG CGAGAACCGG TTGAAAGGCG CGGAGCGGCC AGCCGCGGGG 
GTGGGGAAGG GCTTCCCTGA GGCCAGAGGG AAAGGGCCCG GTCCCCAGAA GCCACCGACG 
GAGGCAGACA AGCCCAATGG CATGAAACGG TCCCCCTCAG CCACTGGGCA GAGTTCTTTC 
CGATCCACGG CCCTCCCGGA AAAGTCTCTG AGCTGCTCCT CCAGCTTCCC TGAAACCAGG 
GCCGGAGTTA GAGAGGCCTC TGCAGCCAGC AGCGACACCT CTTCTGCCAA GGCCGCCGGG 
GGCATGCTGG AGCTTCCAGC CCCCAGCAAC AGGGACCATA GGAAGGCTCA GCCTGCCGGG 
GAGGGCCGAA CCCACATGAC AAAGAGTGAC TCCCTGCCCT CCTTCCGGGT CTCCACCCTG 
CCTCTGGAGT CACACCACCC CGACCCAAAC ACCATGGGCG GGGCCAGCCA CCGGGACAGG 
GCTCTCTCGG TGACTGCCAC CGTAGGGGAA ACCAAAGGGA AGGACCCTGC CCCAGCCCAG 
CCTCCCCCAG CTAGGAAACA GAACGTGGGC AGAGACGTGA CCAAGCCATC CCCAGCCCCA 
AACACTGACC GCCCCATCTC TCTTTCTAAT GAGAAGGACT TTGTGGTACG GCAGAGGCGG 
GGGAAAGAGA GTTTGCGTAG CAGCCCTCAC AAAAAGGCCT TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_011543386.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541688.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X7, mRNA.

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CloneID	OHu79757
Clone ID Related Accession (Same CDS sequence)	XM_011543386.2
Accession Version	XM_011543386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543386.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAAC ATTTATGGAT TTTGTTGGGA AGTCTCAGTT GCCGAACAAG CAACCGGAAA 
AGCTTAATAG GCAATGGGCA GTCACCAGCA TTGCCTCGAC CACACTCACC TCTCTCTGCT 
CATGCAGGAA ATAGCCCTCA AGATAGTCCA AGAAATTTCT CCCCCAGTGC CTCAGCCCAT 
TTTTCATTTG CACGGAGGAC TGATGGACGC CGCTGGTCGT TGGCTTCTCT CCCTTCCTCT 
GGCTATGGGA CAAACACACC CAGCTCTACG GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG 
TTGCATCAGT TACCATACCA ACCAACACCA GACGAGTTAC ACTTCTTATC AAAACATTTC 
TGTACCACCG AAAGCATCGC CACTGAGAAC AGATGCAGGA ACACGCCGAT GCGCCCCCGT 
TCCCGAAGTC TGAGCCCTGG ACGTTCTCCC GCCTGCTGTG ACCATGAAAT AATTATGATG 
AACCATGTCT ACAAAGAAAG GTTCCCAAAG GCTACAGCTC AGATGGAAGA ACGTCTAAAG 
GAAATTATCA CCAGCTACTC TCCTGACAAC GTTCTACCCT TAGCAGATGG AGTGCTTAGT 
TTCACTCACC ACCAGATTAT TGAACTGGCT CGAGATTGCT TGGATAAATC CCACCAGGGC 
CTCATCACCT CACGATACTT CCTTGAATTA CAGCACAAAT TAGATAAGTT GCTACAGGAG 
GCTCATGATC GTTCAGAAAG TGGAGAATTG GCATTTATTA AACAACTAGT TCGAAAGATC 
CTAATTGTTA TTGCCCGCCC TGCTCGGTTA TTAGAGTGCC TGGAATTTGA TCCGGAAGAA 
TTTTACTACC TATTGGAAGC AGCAGAAGGC CATGCCAAAG AAGGACAGGG TATTAAAACC 
GACATTCCCA GGTACATCAT TAGCCAACTG GGACTCAATA AGGATCCCTT GGAAGAAATG 
GCTCATTTGG GAAACTACGA TAGTGGGACA GCAGAAACAC CAGAAACAGA TGAATCAGTG 
AGTAGCTCTA ATGCCTCCCT GAAACTTCGA AGGAAACCTC GGGAAAGTGA TTTTGAAACG 
ATTAAATTGA TTAGCAATGG AGCCTATGGG GCAGTCTACT TTGTTCGGCA TAAAGAATCC 
CGGCAGAGGT TTGCCATGAA GAAGATTAAT AAACAGAACC TCATCCTTCG AAACCAGATC 
CAGCAGGCCT TTGTGGAGCG GGATATCCTG ACTTTTGCAG AAAACCCCTT TGTTGTCAGC 
ATGTATTGCT CCTTTGAAAC AAGGCGCCAC TTGTGCATGG TCATGGAATA TGTGGAAGGG 
GGAGACTGTG CTACTTTAAT GAAAAACATG GGTCCTCTCC CTGTTGATAT GGCCAGAATG 
TACTTTGCTG AGACGGTCTT GGCCTTGGAA TATTTACATA ATTATGGAAT TGTACACAGG 
GATTTGAAAC CAGACAACTT GTTGGTTACC TCCATGGGGC ACATAAAGCT GACAGATTTT 
GGATTATCTA AGGTGGGACT AATGAGCATG ACTACCAACC TTTACGAGGG TCATATTGAG 
AAGGATGCTA GAGAGTTCCT GGATAAACAG GTCTGTGGCA CACCTGAATA CATTGCACCA 
GAAGTGATTC TGAGGCAGGG TTATGGAAAG CCGGTGGACT GGTGGGCCAT GGGGATTATC 
CTCTATGAAT TTCTGGTTGG ATGCGTGCCA TTCTTTGGGG ATACTCCAGA GGAGCTATTT 
GGACAAGTCA TCAGTGATGA GATCAACTGG CCTGAGAAGG ATGAGGCACC CCCACCTGAT 
GCCCAGGATC TGATTACCTT ACTCCTCAGG CAGAATCCCC TGGAGAGGCT GGGAACAGGT 
GGTGCATATG AAGTCAAACA GCATCGATTC TTCCGTTCTT TAGACTGGAA CAGTTTGCTG 
AGACAGAAGG CAGAATTTAT TCCCCAACTG GAATCTGAGG ATGACACAAG TTATTTTGAT 
ACTCGGTCTG AGAAGTATCA TCATATGGAA ACGGAGGAAG AAGATGACAC AAATGATGAA 
GACTTTAATG TGGAAATAAG GCAGTTTTCT TCATGTTCAC ACAGGTTTTC AAAAGTTTTC 
AGCAGTATAG ATCGAATCAC TCAGAATTCA GCAGAAGAGA AGGAAGACTC TGTGGACAAA 
ACCAAAAGCA CCACCTTGCC ATCCACAGAA ACACTGAGCT GGAGTTCAGA ATATTCTGAA 
ATGCAACAGC TATCAACATC CAACTCTTCA GATACTGAAA GCAACAGACA TAAACTCAGT 
TCTGGCCTAC TTCCCAAACT GGCTATTTCA ACAGAGGGAG AGCAAGATGA AGCTGCCTCC 
TGCCCTGGAG ACCCCCATGA GGAGCCAGGA AAGCCAGCCC TTCCTCCTGA AGAGTGTGCC 
CAGGAGGAGC CTGAGGTCAC CACCCCAGCC AGCACCATCA GCAGCTCCAC CCTGTCAGTT 
GGCAGTTTTT CAGAGCACTT GGATCAGATA AATGGACGAA GCGAGTGTGT GGACAGTACA 
GATAATTCCT CAAAGCCATC CAGTGAACCC GCTTCTCACA TGGCTCGGCA GCGATTAGAA 
AGCACAGAAA AAAAGAAAAT CTCGGGGAAA GTCACAAAGT CCCTCTCTGC CAGTGCTCTT 
TCCCTCATGA TCCCAGGAGA TATGTTTGCT GTTTCCCCTC TGGGAAGTCC AATGTCTCCC 
CATTCCCTGT CCTCGGACCC TTCTTCTTCA CGAGATTCCT CTCCCAGCCG AGATTCCTCA 
GCAGCTTCTG CCAGTCCACA TCAGCCGATT GTGATCCACA GTTCGGGGAA GAACTACGGC 
TTTACCATCC GAGCCATCCG GGTGTATGTG GGAGACAGTG ACATCTATAC AGTGCACCAT 
ATCGTCTGGA ATGTAGAAGA AGGAAGTCCG GCATGCCAGG CAGGACTGAA GGCTGGAGAT 
CTTATCACTC ACATCAATGG AGAACCAGTG CATGGACTTG TCCACACAGA AGTTATAGAA 
CTCCTACTGA AGAGTGGGAA TAAGGTGTCA ATCACTACTA CCCCATTTGA AAACACATCA 
ATCAAAACTG GACCAGCCAG GAGAAACAGC TATAAGAGCC GGATGGTGAG GCGGAGCAAG 
AAATCCAAGA AGAAAGAAAG TCTCGAAAGG AGGAGATCTC TTTTCAAAAA GCTAGCCAAG 
CAGCCTTCTC CTTTACTCCA CACCAGCCGA AGTTTCTCCT GCTTGAACAG ATCCCTGTCA 
TCGGGTGAGA GCCTCCCAGG TTCCCCCACT CATAGCTTGT CTCCCCGGTC TCCAACACCA 
AGCTACCGCT CCACCCCTGA CTTCCCATCT GGTACTAATT CCTCCCAGAG CAGCTCCCCT 
AGTTCTAGTG CCCCCAATTC CCCAGCAGGG TCCGGGCACA TCCGGCCCAG CACTCTCCAC 
GGTCTTGCAC CCAAACTCGG CGGGCAGCGG TACCGGTCCG GAAGGCGAAA GTCCGCCGGC 
AACATCCCAC TGTCCCCGCT GGCCCGGACG CCCTCTCCAA CCCCGCAACC CACCTCCCCG 
CAGCGGTCAC CATCCCCTCT TCTGGGACAC TCACTGGGCA ATTCCAAGAT CGCGCAAGCC 
TTTCCCAGCA AGATGCACTC CCCGCCCACC ATCGTCAGAC ACATCGTGAG GCCCAAGAGT 
GCGGAGCCCC CCAGGTCCCC GCTGCTCAAG CGCGTGCAGT CCGAGGAGAA GCTGTCGCCC 
TCTTACGGCA GTGACAAGAA GCACCTGTGC TCCCGCAAGC ACAGCCTGGA GGTGACCCAA 
GAGGAGGTGC AGCGGGAGCA GTCCCAGCGG GAGGCGCCGC TGCAGAGCCT GGATGAGAAC 
GTGTGCGACG TGCCGCCGCT CAGCCGCGCC CGGCCAGTGG AGCAAGGCTG CCTGAAACGC 
CCAGTCTCCC GGAAGGTGGG CCGCCAGGAG TCTGTGGACG ACCTGGACCG CGACAAGCTG 
AAGGCCAAGG TGGTGGTGAA GAAAGCAGAC GGCTTCCCAG AGAAACAGGA ATCCCACCAG 
AAATCCCATG GACCCGGGAG TGATTTGGAA AACTTTGCTC TGTTTAAGCT GGAAGAGAGA 
GAGAAGAAAG TCTATCCGAA GGCTGTGGAA AGGTCAAGTA CTTTTGAAAA CAAAGCGTCT 
ATGCAGGAGG CGCCACCGCT GGGCAGCCTG CTGAAGGATG CTCTTCACAA GCAGGCCAGC 
GTGCGCGCCA GCGAGGGTGC GATGTCGGAT GGCCGGGTGC CTGCGGAGCA CCGCCAGGGT 
GGCGGGGACT TCAGACGGGC CCCCGCTCCT GGCACCCTCC AGGATGGTCT CTGCCACTCC 
CTCGACAGGG GCATCTCTGG GAAGGGGGAA GGCACGGAGA AGTCCTCCCA GGCCAAGGAG 
CTTCTCCGAT GTGAAAAGTT AGACAGCAAG CTGGCCAACA TCGATTACCT CCGAAAGAAA 
ATGTCACTTG AGGACAAAGA GGACAACCTC TGCCCTGTGC TGAAGCCCAA GATGACAGCT 
GGCTCCCACG AATGCCTGCC AGGGAACCCA GTCCGACCCA CGGGTGGGCA GCAGGAGCCC 
CCGCCGGCTT CTGAGAGCCG AGCTTTTGTC AGCAGCACCC ATGCAGCTCA GATGAGTGCC 
GTCTCTTTTG TTCCCCTCAA GGCCTTAACA GGCCGGGTGG ACAGTGGAAC GGAGAAGCCT 
GGCTTGGTTG CTCCTGAGTC CCCTGTTAGG AAGAGCCCCT CCGAGTATAA GCTGGAAGGT 
AGGTCTGTCT CATGCCTGAA GCCGATCGAG GGCACTCTGG ACATTGCTCT CCTGTCCGGA 
CCTCAGGCCT CCAAGACAGA ACTGCCTTCC CCAGAGTCTG CACAGAGCCC CAGCCCAAGT 
GGTGACGTGA GGGCCTCTGT GCCACCAGTT CTCCCCAGCA GCAGTGGGAA AAAGAACGAT 
ACCACCAGTG CAAGAGAGCT TTCTCCTTCC AGCTTAAAGA TGAATAAATC CTACCTGCTG 
GAGCCTTGGT TCCTGCCCCC CAGCCGAGGT CTCCAGAATT CACCAGCAGT TTCCCTGCCT 
GACCCAGAGT TCAAGAGGGA CAGGAAAGGT CCCCATCCTA CTGCCAGGAG CCCTGGAACA 
GTCATGGAAA GCAATCCCCA ACAGAGAGAG GGCAGCTCCC CTAAACACCA AGACCACACC 
ACTGACCCCA AGCTTCTGAC CTGCCTGGGG CAGAACCTCC ACAGCCCTGA CCTGGCCAGG 
CCACGCTGCC CGCTCCCACC TGAAGCTTCC CCCTCAAGGG AGAAGCCAGG CCTGAGGGAA 
TCGTCTGAAA GAGGCCCTCC CACAGCCAGA AGCGAGCGCT CTGCTGCGAG GGCTGACACA 
TGCAGAGAGC CCTCCATGGA ACTGTGCTTT CCAGAAACTG CGAAAACCAG TGACAACTCC 
AAAAATCTCC TCTCTGTGGG AAGGACCCAC CCAGATTTCT ATACACAGAC CCAGGCCATG 
GAGAAAGCAT GGGCGCCGGG TGGGAAAACG AACCACAAAG ATGGCCCAGG TGAGGCGAGG 
CCCCCGCCCA GAGACAACTC CTCTCTGCAC TCAGCTGGAA TTCCCTGTGA GAAGGAGCTG 
GGCAAGGTGA GGCGTGGCGT GGAACCCAAG CCCGAAGCGC TTCTTGCCAG GCGGTCTCTG 
CAGCCACCTG GAATTGAGAG TGAGAAGAGT GAAAAGCTCT CCAGTTTCCC ATCTTTGCAG 
AAAGATGGTG CCAAGGAACC TGAAAGGAAG GAGCAGCCTC TACAAAGGCA TCCCAGCAGC 
ATCCCTCCGC CCCCTCTGAC GGCCAAAGAC CTGTCCAGCC CGGCTGCCAG GCAGCATTGC 
AGTTCCCCAA GCCACGCTTC TGGCAGAGAG CCGGGGGCCA AGCCCAGCAC TGCAGAGCCC 
AGCTCGAGCC CCCAGGACCC TCCCAAGCCT GTTGCTGCGC ACAGTGAAAG CAGCAGCCAC 
AAGCCCCGGC CTGGCCCTGA CCCGGGCCCT CCAAAGACTA AGCACCCCGA CCGGTCCCTC 
TCCTCTCAGA AACCAAGTGT CGGGGCCACA AAGGGCAAAG AGCCTGCCAC TCAGTCCCTC 
GGTGGCTCTA GCAGAGAGGG GAAGGGCCAC AGTAAGAGTG GGCCGGATGT GTTTCCTGCT 
ACCCCAGGCT CCCAGAACAA AGCCAGCGAT GGGATTGGCC AGGGAGAAGG TGGGCCCTCT 
GTCCCACTGC ACACTGACAG GGCTCCTCTA GACGCCAAGC CACAACCCAC CAGTGGTGGG 
CGGCCCCTGG AGGTGCTGGA GAAGCCTGTG CATTTGCCAA GGCCGGGACA CCCAGGGCCT 
AGTGAGCCAG CGGACCAGAA ACTGTCCGCT GTTGGTGAAA AGCAAACCCT GTCTCCAAAG 
CACCCCAAAC CATCCACTGT GAAAGATTGC CCCACCCTGT GCAAACAGAC AGACAACAGA 
CAGACAGACA AAAGCCCGAG TCAGCCGGCC GCCAACACCG ACAGAAGGGC GGAAGGGAAG 
AAATGCACTG AAGCACTTTA TGCTCCAGCA GAGGGCGACA AGCTCGAGGC CGGCCTTTCC 
TTTGTGCATA GCGAGAACCG GTTGAAAGGC GCGGAGCGGC CAGCCGCGGG GGTGGGGAAG 
GGCTTCCCTG AGGCCAGAGG GAAAGGGCCC GGTCCCCAGA AGCCACCGAC GGAGGCAGAC 
AAGCCCAATG GCATGAAACG GTCCCCCTCA GCCACTGGGC AGAGTTCTTT CCGATCCACG 
GCCCTCCCGG AAAAGTCTCT GAGCTGCTCC TCCAGCTTCC CTGAAACCAG GGCCGGAGTT 
AGAGAGGCCT CTGCAGCCAG CAGCGACACC TCTTCTGCCA AGGCCGCCGG GGGCATGCTG 
GAGCTTCCAG CCCCCAGCAA CAGGGACCAT AGGAAGGCTC AGCCTGCCGG GGAGGGCCGA 
ACCCACATGA CAAAGAGTGA CTCCCTGCCC TCCTTCCGGG TCTCCACCCT GCCTCTGGAG 
TCACACCACC CCGACCCAAA CACCATGGGC GGGGCCAGCC ACCGGGACAG GGCTCTCTCG 
GTGACTGCCA CCGTAGGGGA AACCAAAGGG AAGGACCCTG CCCCAGCCCA GCCTCCCCCA 
GCTAGGAAAC AGAACGTGGG CAGAGACGTG ACCAAGCCAT CCCCAGCCCC AAACACTGAC 
CGCCCCATCT CTCTTTCTAA TGAGAAGGAC TTTGTGGTAC GGCAGAGGCG GGGGAAAGAG 
AGTTTGCGTA GCAGCCCTCA CAAAAAGGCC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541688.1
CDS	47..7282
Translation	MAQHLWILLGSLSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_011543386.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543386.2

ORF Insert Sequence:

ATGGCCCAAC ATTTATGGAT TTTGTTGGGA AGTCTCAGTT GCCGAACAAG CAACCGGAAA 
AGCTTAATAG GCAATGGGCA GTCACCAGCA TTGCCTCGAC CACACTCACC TCTCTCTGCT 
CATGCAGGAA ATAGCCCTCA AGATAGTCCA AGAAATTTCT CCCCCAGTGC CTCAGCCCAT 
TTTTCATTTG CACGGAGGAC TGATGGACGC CGCTGGTCGT TGGCTTCTCT CCCTTCCTCT 
GGCTATGGGA CAAACACACC CAGCTCTACG GTCTCTTCAT CCTGTTCCTC CCAGGAGAAG 
TTGCATCAGT TACCATACCA ACCAACACCA GACGAGTTAC ACTTCTTATC AAAACATTTC 
TGTACCACCG AAAGCATCGC CACTGAGAAC AGATGCAGGA ACACGCCGAT GCGCCCCCGT 
TCCCGAAGTC TGAGCCCTGG ACGTTCTCCC GCCTGCTGTG ACCATGAAAT AATTATGATG 
AACCATGTCT ACAAAGAAAG GTTCCCAAAG GCTACAGCTC AGATGGAAGA ACGTCTAAAG 
GAAATTATCA CCAGCTACTC TCCTGACAAC GTTCTACCCT TAGCAGATGG AGTGCTTAGT 
TTCACTCACC ACCAGATTAT TGAACTGGCT CGAGATTGCT TGGATAAATC CCACCAGGGC 
CTCATCACCT CACGATACTT CCTTGAATTA CAGCACAAAT TAGATAAGTT GCTACAGGAG 
GCTCATGATC GTTCAGAAAG TGGAGAATTG GCATTTATTA AACAACTAGT TCGAAAGATC 
CTAATTGTTA TTGCCCGCCC TGCTCGGTTA TTAGAGTGCC TGGAATTTGA TCCGGAAGAA 
TTTTACTACC TATTGGAAGC AGCAGAAGGC CATGCCAAAG AAGGACAGGG TATTAAAACC 
GACATTCCCA GGTACATCAT TAGCCAACTG GGACTCAATA AGGATCCCTT GGAAGAAATG 
GCTCATTTGG GAAACTACGA TAGTGGGACA GCAGAAACAC CAGAAACAGA TGAATCAGTG 
AGTAGCTCTA ATGCCTCCCT GAAACTTCGA AGGAAACCTC GGGAAAGTGA TTTTGAAACG 
ATTAAATTGA TTAGCAATGG AGCCTATGGG GCAGTCTACT TTGTTCGGCA TAAAGAATCC 
CGGCAGAGGT TTGCCATGAA GAAGATTAAT AAACAGAACC TCATCCTTCG AAACCAGATC 
CAGCAGGCCT TTGTGGAGCG GGATATCCTG ACTTTTGCAG AAAACCCCTT TGTTGTCAGC 
ATGTATTGCT CCTTTGAAAC AAGGCGCCAC TTGTGCATGG TCATGGAATA TGTGGAAGGG 
GGAGACTGTG CTACTTTAAT GAAAAACATG GGTCCTCTCC CTGTTGATAT GGCCAGAATG 
TACTTTGCTG AGACGGTCTT GGCCTTGGAA TATTTACATA ATTATGGAAT TGTACACAGG 
GATTTGAAAC CAGACAACTT GTTGGTTACC TCCATGGGGC ACATAAAGCT GACAGATTTT 
GGATTATCTA AGGTGGGACT AATGAGCATG ACTACCAACC TTTACGAGGG TCATATTGAG 
AAGGATGCTA GAGAGTTCCT GGATAAACAG GTCTGTGGCA CACCTGAATA CATTGCACCA 
GAAGTGATTC TGAGGCAGGG TTATGGAAAG CCGGTGGACT GGTGGGCCAT GGGGATTATC 
CTCTATGAAT TTCTGGTTGG ATGCGTGCCA TTCTTTGGGG ATACTCCAGA GGAGCTATTT 
GGACAAGTCA TCAGTGATGA GATCAACTGG CCTGAGAAGG ATGAGGCACC CCCACCTGAT 
GCCCAGGATC TGATTACCTT ACTCCTCAGG CAGAATCCCC TGGAGAGGCT GGGAACAGGT 
GGTGCATATG AAGTCAAACA GCATCGATTC TTCCGTTCTT TAGACTGGAA CAGTTTGCTG 
AGACAGAAGG CAGAATTTAT TCCCCAACTG GAATCTGAGG ATGACACAAG TTATTTTGAT 
ACTCGGTCTG AGAAGTATCA TCATATGGAA ACGGAGGAAG AAGATGACAC AAATGATGAA 
GACTTTAATG TGGAAATAAG GCAGTTTTCT TCATGTTCAC ACAGGTTTTC AAAAGTTTTC 
AGCAGTATAG ATCGAATCAC TCAGAATTCA GCAGAAGAGA AGGAAGACTC TGTGGACAAA 
ACCAAAAGCA CCACCTTGCC ATCCACAGAA ACACTGAGCT GGAGTTCAGA ATATTCTGAA 
ATGCAACAGC TATCAACATC CAACTCTTCA GATACTGAAA GCAACAGACA TAAACTCAGT 
TCTGGCCTAC TTCCCAAACT GGCTATTTCA ACAGAGGGAG AGCAAGATGA AGCTGCCTCC 
TGCCCTGGAG ACCCCCATGA GGAGCCAGGA AAGCCAGCCC TTCCTCCTGA AGAGTGTGCC 
CAGGAGGAGC CTGAGGTCAC CACCCCAGCC AGCACCATCA GCAGCTCCAC CCTGTCAGTT 
GGCAGTTTTT CAGAGCACTT GGATCAGATA AATGGACGAA GCGAGTGTGT GGACAGTACA 
GATAATTCCT CAAAGCCATC CAGTGAACCC GCTTCTCACA TGGCTCGGCA GCGATTAGAA 
AGCACAGAAA AAAAGAAAAT CTCGGGGAAA GTCACAAAGT CCCTCTCTGC CAGTGCTCTT 
TCCCTCATGA TCCCAGGAGA TATGTTTGCT GTTTCCCCTC TGGGAAGTCC AATGTCTCCC 
CATTCCCTGT CCTCGGACCC TTCTTCTTCA CGAGATTCCT CTCCCAGCCG AGATTCCTCA 
GCAGCTTCTG CCAGTCCACA TCAGCCGATT GTGATCCACA GTTCGGGGAA GAACTACGGC 
TTTACCATCC GAGCCATCCG GGTGTATGTG GGAGACAGTG ACATCTATAC AGTGCACCAT 
ATCGTCTGGA ATGTAGAAGA AGGAAGTCCG GCATGCCAGG CAGGACTGAA GGCTGGAGAT 
CTTATCACTC ACATCAATGG AGAACCAGTG CATGGACTTG TCCACACAGA AGTTATAGAA 
CTCCTACTGA AGAGTGGGAA TAAGGTGTCA ATCACTACTA CCCCATTTGA AAACACATCA 
ATCAAAACTG GACCAGCCAG GAGAAACAGC TATAAGAGCC GGATGGTGAG GCGGAGCAAG 
AAATCCAAGA AGAAAGAAAG TCTCGAAAGG AGGAGATCTC TTTTCAAAAA GCTAGCCAAG 
CAGCCTTCTC CTTTACTCCA CACCAGCCGA AGTTTCTCCT GCTTGAACAG ATCCCTGTCA 
TCGGGTGAGA GCCTCCCAGG TTCCCCCACT CATAGCTTGT CTCCCCGGTC TCCAACACCA 
AGCTACCGCT CCACCCCTGA CTTCCCATCT GGTACTAATT CCTCCCAGAG CAGCTCCCCT 
AGTTCTAGTG CCCCCAATTC CCCAGCAGGG TCCGGGCACA TCCGGCCCAG CACTCTCCAC 
GGTCTTGCAC CCAAACTCGG CGGGCAGCGG TACCGGTCCG GAAGGCGAAA GTCCGCCGGC 
AACATCCCAC TGTCCCCGCT GGCCCGGACG CCCTCTCCAA CCCCGCAACC CACCTCCCCG 
CAGCGGTCAC CATCCCCTCT TCTGGGACAC TCACTGGGCA ATTCCAAGAT CGCGCAAGCC 
TTTCCCAGCA AGATGCACTC CCCGCCCACC ATCGTCAGAC ACATCGTGAG GCCCAAGAGT 
GCGGAGCCCC CCAGGTCCCC GCTGCTCAAG CGCGTGCAGT CCGAGGAGAA GCTGTCGCCC 
TCTTACGGCA GTGACAAGAA GCACCTGTGC TCCCGCAAGC ACAGCCTGGA GGTGACCCAA 
GAGGAGGTGC AGCGGGAGCA GTCCCAGCGG GAGGCGCCGC TGCAGAGCCT GGATGAGAAC 
GTGTGCGACG TGCCGCCGCT CAGCCGCGCC CGGCCAGTGG AGCAAGGCTG CCTGAAACGC 
CCAGTCTCCC GGAAGGTGGG CCGCCAGGAG TCTGTGGACG ACCTGGACCG CGACAAGCTG 
AAGGCCAAGG TGGTGGTGAA GAAAGCAGAC GGCTTCCCAG AGAAACAGGA ATCCCACCAG 
AAATCCCATG GACCCGGGAG TGATTTGGAA AACTTTGCTC TGTTTAAGCT GGAAGAGAGA 
GAGAAGAAAG TCTATCCGAA GGCTGTGGAA AGGTCAAGTA CTTTTGAAAA CAAAGCGTCT 
ATGCAGGAGG CGCCACCGCT GGGCAGCCTG CTGAAGGATG CTCTTCACAA GCAGGCCAGC 
GTGCGCGCCA GCGAGGGTGC GATGTCGGAT GGCCGGGTGC CTGCGGAGCA CCGCCAGGGT 
GGCGGGGACT TCAGACGGGC CCCCGCTCCT GGCACCCTCC AGGATGGTCT CTGCCACTCC 
CTCGACAGGG GCATCTCTGG GAAGGGGGAA GGCACGGAGA AGTCCTCCCA GGCCAAGGAG 
CTTCTCCGAT GTGAAAAGTT AGACAGCAAG CTGGCCAACA TCGATTACCT CCGAAAGAAA 
ATGTCACTTG AGGACAAAGA GGACAACCTC TGCCCTGTGC TGAAGCCCAA GATGACAGCT 
GGCTCCCACG AATGCCTGCC AGGGAACCCA GTCCGACCCA CGGGTGGGCA GCAGGAGCCC 
CCGCCGGCTT CTGAGAGCCG AGCTTTTGTC AGCAGCACCC ATGCAGCTCA GATGAGTGCC 
GTCTCTTTTG TTCCCCTCAA GGCCTTAACA GGCCGGGTGG ACAGTGGAAC GGAGAAGCCT 
GGCTTGGTTG CTCCTGAGTC CCCTGTTAGG AAGAGCCCCT CCGAGTATAA GCTGGAAGGT 
AGGTCTGTCT CATGCCTGAA GCCGATCGAG GGCACTCTGG ACATTGCTCT CCTGTCCGGA 
CCTCAGGCCT CCAAGACAGA ACTGCCTTCC CCAGAGTCTG CACAGAGCCC CAGCCCAAGT 
GGTGACGTGA GGGCCTCTGT GCCACCAGTT CTCCCCAGCA GCAGTGGGAA AAAGAACGAT 
ACCACCAGTG CAAGAGAGCT TTCTCCTTCC AGCTTAAAGA TGAATAAATC CTACCTGCTG 
GAGCCTTGGT TCCTGCCCCC CAGCCGAGGT CTCCAGAATT CACCAGCAGT TTCCCTGCCT 
GACCCAGAGT TCAAGAGGGA CAGGAAAGGT CCCCATCCTA CTGCCAGGAG CCCTGGAACA 
GTCATGGAAA GCAATCCCCA ACAGAGAGAG GGCAGCTCCC CTAAACACCA AGACCACACC 
ACTGACCCCA AGCTTCTGAC CTGCCTGGGG CAGAACCTCC ACAGCCCTGA CCTGGCCAGG 
CCACGCTGCC CGCTCCCACC TGAAGCTTCC CCCTCAAGGG AGAAGCCAGG CCTGAGGGAA 
TCGTCTGAAA GAGGCCCTCC CACAGCCAGA AGCGAGCGCT CTGCTGCGAG GGCTGACACA 
TGCAGAGAGC CCTCCATGGA ACTGTGCTTT CCAGAAACTG CGAAAACCAG TGACAACTCC 
AAAAATCTCC TCTCTGTGGG AAGGACCCAC CCAGATTTCT ATACACAGAC CCAGGCCATG 
GAGAAAGCAT GGGCGCCGGG TGGGAAAACG AACCACAAAG ATGGCCCAGG TGAGGCGAGG 
CCCCCGCCCA GAGACAACTC CTCTCTGCAC TCAGCTGGAA TTCCCTGTGA GAAGGAGCTG 
GGCAAGGTGA GGCGTGGCGT GGAACCCAAG CCCGAAGCGC TTCTTGCCAG GCGGTCTCTG 
CAGCCACCTG GAATTGAGAG TGAGAAGAGT GAAAAGCTCT CCAGTTTCCC ATCTTTGCAG 
AAAGATGGTG CCAAGGAACC TGAAAGGAAG GAGCAGCCTC TACAAAGGCA TCCCAGCAGC 
ATCCCTCCGC CCCCTCTGAC GGCCAAAGAC CTGTCCAGCC CGGCTGCCAG GCAGCATTGC 
AGTTCCCCAA GCCACGCTTC TGGCAGAGAG CCGGGGGCCA AGCCCAGCAC TGCAGAGCCC 
AGCTCGAGCC CCCAGGACCC TCCCAAGCCT GTTGCTGCGC ACAGTGAAAG CAGCAGCCAC 
AAGCCCCGGC CTGGCCCTGA CCCGGGCCCT CCAAAGACTA AGCACCCCGA CCGGTCCCTC 
TCCTCTCAGA AACCAAGTGT CGGGGCCACA AAGGGCAAAG AGCCTGCCAC TCAGTCCCTC 
GGTGGCTCTA GCAGAGAGGG GAAGGGCCAC AGTAAGAGTG GGCCGGATGT GTTTCCTGCT 
ACCCCAGGCT CCCAGAACAA AGCCAGCGAT GGGATTGGCC AGGGAGAAGG TGGGCCCTCT 
GTCCCACTGC ACACTGACAG GGCTCCTCTA GACGCCAAGC CACAACCCAC CAGTGGTGGG 
CGGCCCCTGG AGGTGCTGGA GAAGCCTGTG CATTTGCCAA GGCCGGGACA CCCAGGGCCT 
AGTGAGCCAG CGGACCAGAA ACTGTCCGCT GTTGGTGAAA AGCAAACCCT GTCTCCAAAG 
CACCCCAAAC CATCCACTGT GAAAGATTGC CCCACCCTGT GCAAACAGAC AGACAACAGA 
CAGACAGACA AAAGCCCGAG TCAGCCGGCC GCCAACACCG ACAGAAGGGC GGAAGGGAAG 
AAATGCACTG AAGCACTTTA TGCTCCAGCA GAGGGCGACA AGCTCGAGGC CGGCCTTTCC 
TTTGTGCATA GCGAGAACCG GTTGAAAGGC GCGGAGCGGC CAGCCGCGGG GGTGGGGAAG 
GGCTTCCCTG AGGCCAGAGG GAAAGGGCCC GGTCCCCAGA AGCCACCGAC GGAGGCAGAC 
AAGCCCAATG GCATGAAACG GTCCCCCTCA GCCACTGGGC AGAGTTCTTT CCGATCCACG 
GCCCTCCCGG AAAAGTCTCT GAGCTGCTCC TCCAGCTTCC CTGAAACCAG GGCCGGAGTT 
AGAGAGGCCT CTGCAGCCAG CAGCGACACC TCTTCTGCCA AGGCCGCCGG GGGCATGCTG 
GAGCTTCCAG CCCCCAGCAA CAGGGACCAT AGGAAGGCTC AGCCTGCCGG GGAGGGCCGA 
ACCCACATGA CAAAGAGTGA CTCCCTGCCC TCCTTCCGGG TCTCCACCCT GCCTCTGGAG 
TCACACCACC CCGACCCAAA CACCATGGGC GGGGCCAGCC ACCGGGACAG GGCTCTCTCG 
GTGACTGCCA CCGTAGGGGA AACCAAAGGG AAGGACCCTG CCCCAGCCCA GCCTCCCCCA 
GCTAGGAAAC AGAACGTGGG CAGAGACGTG ACCAAGCCAT CCCCAGCCCC AAACACTGAC 
CGCCCCATCT CTCTTTCTAA TGAGAAGGAC TTTGTGGTAC GGCAGAGGCG GGGGAAAGAG 
AGTTTGCGTA GCAGCCCTCA CAAAAAGGCC TTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001290228.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277157.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 6, mRNA.

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CloneID	OHu15018
Clone ID Related Accession (Same CDS sequence)	NM_001290228.2 , NM_001290228.1
Accession Version	NM_001290228.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092373.2, CB044105.1, AY439222.1, BC033215.1 and AC008872.6. On Jun 2, 2019 this sequence version replaced NM_001290228.1. Transcript Variant: This variant (6) uses a distinct 3' coding region and 3' UTR compared to variant 3. The encoded protein (isoform f) has a shorter and distinct C-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY439222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTCCCTGT GTGTCCAAAT GCTGGGAGAA CATCACCCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277157.1
CDS	264..980
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSFPVCPNAGRTSPLG

Target ORF information:

RefSeq Version	NM_001290228.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290228.2

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTCCCTGT GTGTCCAAAT GCTGGGAGAA CATCACCCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001290228.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001277157.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu15018
Clone ID Related Accession (Same CDS sequence)	NM_001290228.2 , NM_001290228.1
Accession Version	NM_001290228.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092373.2, CB044105.1, AY439222.1, BC033215.1 and AC008872.6. Transcript Variant: This variant (6) uses a distinct 3' coding region and 3' UTR compared to variant 3. The encoded protein (isoform f) has a shorter and distinct C-terminus compared to isoform c. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: AY439222.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142680 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTCCCTGT GTGTCCAAAT GCTGGGAGAA CATCACCCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001277157.1
CDS	309..1025
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSFPVCPNAGRTSPLG

Target ORF information:

RefSeq Version	NM_001290228.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290228.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTCCCTGT GTGTCCAAAT GCTGGGAGAA CATCACCCCT TGGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001164664.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158136.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 3, mRNA.

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CloneID	OHu21594
Clone ID Related Accession (Same CDS sequence)	NM_001164664.1 , NM_001164664.2
Accession Version	NM_001164664.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092373.2, AC016634.7, AC008872.6, AC008920.7 and AC044799.5. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTTTGCCG AACAAGCAAC CGGAAAAGCT TAATAGGCAA TGGGCAGTCA 
CCAGCATTGC CTCGACCACA CTCACCTCTC TCTGCTCATG CAGGAAATAG CCCTCAAGAT 
AGTCCAAGAA ATTTCTCCCC CAGTGCCTCA GCCCATTTTT CATTTGCACG GAGGACTGAT 
GGACGCCGCT GGTCGTTGGC TTCTCTCCCT TCCTCTGGCT ATGGGACAAA CACACCCAGC 
TCTACGGTCT CTTCATCCTG TTCCTCCCAG GAGAAGTTGC ATCAGTTACC ATACCAACCA 
ACACCAGACG AGTTACACTT CTTATCAAAA CATTTCTGTA CCACCGAAAG CATCGCCACT 
GAGAACAGAT GCAGGAACAC GCCGATGCGC CCCCGTTCCC GAAGTCTGAG CCCTGGACGT 
TCTCCCGCCT GCTGTGACCA TGAAATAATT ATGATGAACC ATGTCTACAA AGAAAGGTTC 
CCAAAGGCTA CAGCTCAGAT GGAAGAACGT CTAAAGGAAA TTATCACCAG CTACTCTCCT 
GACAACGTTC TACCCTTAGC AGATGGAGTG CTTAGTTTCA CTCACCACCA GATTATTGAA 
CTGGCTCGAG ATTGCTTGGA TAAATCCCAC CAGGGCCTCA TCACCTCACG ATACTTCCTT 
GAATTACAGC ACAAATTAGA TAAGTTGCTA CAGGAGGCTC ATGATCGTTC AGAAAGTGGA 
GAATTGGCAT TTATTAAACA ACTAGTTCGA AAGATCCTAA TTGTTATTGC CCGCCCTGCT 
CGGTTATTAG AGTGCCTGGA ATTTGATCCG GAAGAATTTT ACTACCTATT GGAAGCAGCA 
GAAGGCCATG CCAAAGAAGG ACAGGGTATT AAAACCGACA TTCCCAGGTA CATCATTAGC 
CAACTGGGAC TCAATAAGGA TCCCTTGGAA GAAATGGCTC ATTTGGGAAA CTACGATAGT 
GGGACAGCAG AAACACCAGA AACAGATGAA TCAGTGAGTA GCTCTAATGC CTCCCTGAAA 
CTTCGAAGGA AACCTCGGGA AAGTGATTTT GAAACGATTA AATTGATTAG CAATGGAGCC 
TATGGGGCAG TCTACTTTGT TCGGCATAAA GAATCCCGGC AGAGGTTTGC CATGAAGAAG 
ATTAATAAAC AGAACCTCAT CCTTCGAAAC CAGATCCAGC AGGCCTTTGT GGAGCGGGAT 
ATCCTGACTT TTGCAGAAAA CCCCTTTGTT GTCAGCATGT ATTGCTCCTT TGAAACAAGG 
CGCCACTTGT GCATGGTCAT GGAATATGTG GAAGGGGGAG ACTGTGCTAC TTTAATGAAA 
AACATGGGTC CTCTCCCTGT TGATATGGCC AGAATGTACT TTGCTGAGAC GGTCTTGGCC 
TTGGAATATT TACATAATTA TGGAATTGTA CACAGGGATT TGAAACCAGA CAACTTGTTG 
GTTACCTCCA TGGGGCACAT AAAGCTGACA GATTTTGGAT TATCTAAGGT GGGACTAATG 
AGCATGACTA CCAACCTTTA CGAGGGTCAT ATTGAGAAGG ATGCTAGAGA GTTCCTGGAT 
AAACAGGTCT GTGGCACACC TGAATACATT GCACCAGAAG TGATTCTGAG GCAGGGTTAT 
GGAAAGCCGG TGGACTGGTG GGCCATGGGG ATTATCCTCT ATGAATTTCT GGTTGGATGC 
GTGCCATTCT TTGGGGATAC TCCAGAGGAG CTATTTGGAC AAGTCATCAG TGATGAGATC 
AACTGGCCTG AGAAGGATGA GGCACCCCCA CCTGATGCCC AGGATCTGAT TACCTTACTC 
CTCAGGCAGA ATCCCCTGGA GAGGCTGGGA ACAGGTGGTG CATATGAAGT CAAACAGCAT 
CGATTCTTCC GTTCTTTAGA CTGGAACAGT TTGCTGAGAC AGAAGGCAGA ATTTATTCCC 
CAACTGGAAT CTGAGGATGA CACAAGTTAT TTTGATACTC GGTCTGAGAA GTATCATCAT 
ATGGAAACGG AGGAAGAAGA TGACACAAAT GATGAAGACT TTAATGTGGA AATAAGGCAG 
TTTTCTTCAT GTTCACACAG GTTTTCAAAA GTTTTCAGCA GTATAGATCG AATCACTCAG 
AATTCAGCAG AAGAGAAGGA AGACTCTGTG GACAAAACCA AAAGCACCAC CTTGCCATCC 
ACAGAAACAC TGAGCTGGAG TTCAGAATAT TCTGAAATGC AACAGCTATC AACATCCAAC 
TCTTCAGATA CTGAAAGCAA CAGACATAAA CTCAGTTCTG GCCTACTTCC CAAACTGGCT 
ATTTCAACAG AGGGAGAGCA AGATGAAGCT GCCTCCTGCC CTGGAGACCC CCATGAGGAG 
CCAGGAAAGC CAGCCCTTCC TCCTGAAGAG TGTGCCCAGG AGGAGCCTGA GGTCACCACC 
CCAGCCAGCA CCATCAGCAG CTCCACCCTG TCAGTTGGCA GTTTTTCAGA GCACTTGGAT 
CAGATAAATG GACGAAGCGA GTGTGTGGAC AGTACAGATA ATTCCTCAAA GCCATCCAGT 
GAACCCGCTT CTCACATGGC TCGGCAGCGA TTAGAAAGCA CAGAAAAAAA GAAAATCTCG 
GGGAAAGTCA CAAAGTCCCT CTCTGCCAGT GCTCTTTCCC TCATGATCCC AGGAGATATG 
TTTGCTGTTT CCCCTCTGGG AAGTCCAATG TCTCCCCATT CCCTGTCCTC GGACCCTTCT 
TCTTCACGAG ATTCCTCTCC CAGCCGAGAT TCCTCAGCAG CTTCTGCCAG TCCACATCAG 
CCGATTGTGA TCCACAGTTC GGGGAAGAAC TACGGCTTTA CCATCCGAGC CATCCGGGTG 
TATGTGGGAG ACAGTGACAT CTATACAGTG CACCATATCG TCTGGAATGT AGAAGAAGGA 
AGTCCGGCAT GCCAGGCAGG ACTGAAGGCT GGAGATCTTA TCACTCACAT CAATGGAGAA 
CCAGTGCATG GACTTGTCCA CACAGAAGTT ATAGAACTCC TACTGAAGAG TGGGAATAAG 
GTGTCAATCA CTACTACCCC ATTTGAAAAC ACATCAATCA AAACTGGACC AGCCAGGAGA 
AACAGCTATA AGAGCCGGAT GGTGAGGCGG AGCAAGAAAT CCAAGAAGAA AGAAAGTCTC 
GAAAGGAGGA GATCTCTTTT CAAAAAGCTA GCCAAGCAGC CTTCTCCTTT ACTCCACACC 
AGCCGAAGTT TCTCCTGCTT GAACAGATCC CTGTCATCGG GTGAGAGCCT CCCAGGTTCC 
CCCACTCATA GCTTGTCTCC CCGGTCTCCA ACACCAAGCT ACCGCTCCAC CCCTGACTTC 
CCATCTGGTA CTAATTCCTC CCAGAGCAGC TCCCCTAGTT CTAGTGCCCC CAATTCCCCA 
GCAGGGTCCG GGCACATCCG GCCCAGCACT CTCCACGGTC TTGCACCCAA ACTCGGCGGG 
CAGCGGTACC GGTCCGGAAG GCGAAAGTCC GCCGGCAACA TCCCACTGTC CCCGCTGGCC 
CGGACGCCCT CTCCAACCCC GCAACCCACC TCCCCGCAGC GGTCACCATC CCCTCTTCTG 
GGACACTCAC TGGGCAATTC CAAGATCGCG CAAGCCTTTC CCAGCAAGAT GCACTCCCCG 
CCCACCATCG TCAGACACAT CGTGAGGCCC AAGAGTGCGG AGCCCCCCAG GTCCCCGCTG 
CTCAAGCGCG TGCAGTCCGA GGAGAAGCTG TCGCCCTCTT ACGGCAGTGA CAAGAAGCAC 
CTGTGCTCCC GCAAGCACAG CCTGGAGGTG ACCCAAGAGG AGGTGCAGCG GGAGCAGTCC 
CAGCGGGAGG CGCCGCTGCA GAGCCTGGAT GAGAACGTGT GCGACGTGCC GCCGCTCAGC 
CGCGCCCGGC CAGTGGAGCA AGGCTGCCTG AAACGCCCAG TCTCCCGGAA GGTGGGCCGC 
CAGGAGTCTG TGGACGACCT GGACCGCGAC AAGCTGAAGG CCAAGGTGGT GGTGAAGAAA 
GCAGACGGCT TCCCAGAGAA ACAGGAATCC CACCAGAAAT CCCATGGACC CGGGAGTGAT 
TTGGAAAACT TTGCTCTGTT TAAGCTGGAA GAGAGAGAGA AGAAAGTCTA TCCGAAGGCT 
GTGGAAAGGT CAAGTACTTT TGAAAACAAA GCGTCTATGC AGGAGGCGCC ACCGCTGGGC 
AGCCTGCTGA AGGATGCTCT TCACAAGCAG GCCAGCGTGC GCGCCAGCGA GGGTGCGATG 
TCGGATGGCC GGGTGCCTGC GGAGCACCGC CAGGGTGGCG GGGACTTCAG ACGGGCCCCC 
GCTCCTGGCA CCCTCCAGGA TGGTCTCTGC CACTCCCTCG ACAGGGGCAT CTCTGGGAAG 
GGGGAAGGCA CGGAGAAGTC CTCCCAGGCC AAGGAGCTTC TCCGATGTGA AAAGTTAGAC 
AGCAAGCTGG CCAACATCGA TTACCTCCGA AAGAAAATGT CACTTGAGGA CAAAGAGGAC 
AACCTCTGCC CTGTGCTGAA GCCCAAGATG ACAGCTGGCT CCCACGAATG CCTGCCAGGG 
AACCCAGTCC GACCCACGGG TGGGCAGCAG GAGCCCCCGC CGGCTTCTGA GAGCCGAGCT 
TTTGTCAGCA GCACCCATGC AGCTCAGATG AGTGCCGTCT CTTTTGTTCC CCTCAAGGCC 
TTAACAGGCC GGGTGGACAG TGGAACGGAG AAGCCTGGCT TGGTTGCTCC TGAGTCCCCT 
GTTAGGAAGA GCCCCTCCGA GTATAAGCTG GAAGGTAGGT CTGTCTCATG CCTGAAGCCG 
ATCGAGGGCA CTCTGGACAT TGCTCTCCTG TCCGGACCTC AGGCCTCCAA GACAGAACTG 
CCTTCCCCAG AGTCTGCACA GAGCCCCAGC CCAAGTGGTG ACGTGAGGGC CTCTGTGCCA 
CCAGTTCTCC CCAGCAGCAG TGGGAAAAAG AACGATACCA CCAGTGCAAG AGAGCTTTCT 
CCTTCCAGCT TAAAGATGAA TAAATCCTAC CTGCTGGAGC CTTGGTTCCT GCCCCCCAGC 
CGAGGTCTCC AGAATTCACC AGCAGTTTCC CTGCCTGACC CAGAGTTCAA GAGGGACAGG 
AAAGGTCCCC ATCCTACTGC CAGGAGCCCT GGAACAGTCA TGGAAAGCAA TCCCCAACAG 
AGAGAGGGCA GCTCCCCTAA ACACCAAGAC CACACCACTG ACCCCAAGCT TCTGACCTGC 
CTGGGGCAGA ACCTCCACAG CCCTGACCTG GCCAGGCCAC GCTGCCCGCT CCCACCTGAA 
GCTTCCCCCT CAAGGGAGAA GCCAGGCCTG AGGGAATCGT CTGAAAGAGG CCCTCCCACA 
GCCAGAAGCG AGCGCTCTGC TGCGAGGGCT GACACATGCA GAGAGCCCTC CATGGAACTG 
TGCTTTCCAG AAACTGCGAA AACCAGTGAC AACTCCAAAA ATCTCCTCTC TGTGGGAAGG 
ACCCACCCAG ATTTCTATAC ACAGACCCAG GCCATGGAGA AAGCATGGGC GCCGGGTGGG 
AAAACGAACC ACAAAGATGG CCCAGGTGAG GCGAGGCCCC CGCCCAGAGA CAACTCCTCT 
CTGCACTCAG CTGGAATTCC CTGTGAGAAG GAGCTGGGCA AGGTGAGGCG TGGCGTGGAA 
CCCAAGCCCG AAGCGCTTCT TGCCAGGCGG TCTCTGCAGC CACCTGGAAT TGAGAGTGAG 
AAGAGTGAAA AGCTCTCCAG TTTCCCATCT TTGCAGAAAG ATGGTGCCAA GGAACCTGAA 
AGGAAGGAGC AGCCTCTACA AAGGCATCCC AGCAGCATCC CTCCGCCCCC TCTGACGGCC 
AAAGACCTGT CCAGCCCGGC TGCCAGGCAG CATTGCAGTT CCCCAAGCCA CGCTTCTGGC 
AGAGAGCCGG GGGCCAAGCC CAGCACTGCA GAGCCCAGCT CGAGCCCCCA GGACCCTCCC 
AAGCCTGTTG CTGCGCACAG TGAAAGCAGC AGCCACAAGC CCCGGCCTGG CCCTGACCCG 
GGCCCTCCAA AGACTAAGCA CCCCGACCGG TCCCTCTCCT CTCAGAAACC AAGTGTCGGG 
GCCACAAAGG GCAAAGAGCC TGCCACTCAG TCCCTCGGTG GCTCTAGCAG AGAGGGGAAG 
GGCCACAGTA AGAGTGGGCC GGATGTGTTT CCTGCTACCC CAGGCTCCCA GAACAAAGCC 
AGCGATGGGA TTGGCCAGGG AGAAGGTGGG CCCTCTGTCC CACTGCACAC TGACAGGGCT 
CCTCTAGACG CCAAGCCACA ACCCACCAGT GGTGGGCGGC CCCTGGAGGT GCTGGAGAAG 
CCTGTGCATT TGCCAAGGCC GGGACACCCA GGGCCTAGTG AGCCAGCGGA CCAGAAACTG 
TCCGCTGTTG GTGAAAAGCA AACCCTGTCT CCAAAGCACC CCAAACCATC CACTGTGAAA 
GATTGCCCCA CCCTGTGCAA ACAGACAGAC AACAGACAGA CAGACAAAAG CCCGAGTCAG 
CCGGCCGCCA ACACCGACAG AAGGGCGGAA GGGAAGAAAT GCACTGAAGC ACTTTATGCT 
CCAGCAGAGG GCGACAAGCT CGAGGCCGGC CTTTCCTTTG TGCATAGCGA GAACCGGTTG 
AAAGGCGCGG AGCGGCCAGC CGCGGGGGTG GGGAAGGGCT TCCCTGAGGC CAGAGGGAAA 
GGGCCCGGTC CCCAGAAGCC ACCGACGGAG GCAGACAAGC CCAATGGCAT GAAACGGTCC 
CCCTCAGCCA CTGGGCAGAG TTCTTTCCGA TCCACGGCCC TCCCGGAAAA GTCTCTGAGC 
TGCTCCTCCA GCTTCCCTGA AACCAGGGCC GGAGTTAGAG AGGCCTCTGC AGCCAGCAGC 
GACACCTCTT CTGCCAAGGC CGCCGGGGGC ATGCTGGAGC TTCCAGCCCC CAGCAACAGG 
GACCATAGGA AGGCTCAGCC TGCCGGGGAG GGCCGAACCC ACATGACAAA GAGTGACTCC 
CTGCCCTCCT TCCGGGTCTC CACCCTGCCT CTGGAGTCAC ACCACCCCGA CCCAAACACC 
ATGGGCGGGG CCAGCCACCG GGACAGGGCT CTCTCGGTGA CTGCCACCGT AGGGGAAACC 
AAAGGGAAGG ACCCTGCCCC AGCCCAGCCT CCCCCAGCTA GGAAACAGAA CGTGGGCAGA 
GACGTGACCA AGCCATCCCC AGCCCCAAAC ACTGACCGCC CCATCTCTCT TTCTAATGAG 
AAGGACTTTG TGGTACGGCA GAGGCGGGGG AAAGAGAGTT TGCGTAGCAG CCCTCACAAA 
AAGGCCTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158136.1
CDS	309..8180
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSFCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001164664.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164664.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTTTGCCG AACAAGCAAC CGGAAAAGCT TAATAGGCAA TGGGCAGTCA 
CCAGCATTGC CTCGACCACA CTCACCTCTC TCTGCTCATG CAGGAAATAG CCCTCAAGAT 
AGTCCAAGAA ATTTCTCCCC CAGTGCCTCA GCCCATTTTT CATTTGCACG GAGGACTGAT 
GGACGCCGCT GGTCGTTGGC TTCTCTCCCT TCCTCTGGCT ATGGGACAAA CACACCCAGC 
TCTACGGTCT CTTCATCCTG TTCCTCCCAG GAGAAGTTGC ATCAGTTACC ATACCAACCA 
ACACCAGACG AGTTACACTT CTTATCAAAA CATTTCTGTA CCACCGAAAG CATCGCCACT 
GAGAACAGAT GCAGGAACAC GCCGATGCGC CCCCGTTCCC GAAGTCTGAG CCCTGGACGT 
TCTCCCGCCT GCTGTGACCA TGAAATAATT ATGATGAACC ATGTCTACAA AGAAAGGTTC 
CCAAAGGCTA CAGCTCAGAT GGAAGAACGT CTAAAGGAAA TTATCACCAG CTACTCTCCT 
GACAACGTTC TACCCTTAGC AGATGGAGTG CTTAGTTTCA CTCACCACCA GATTATTGAA 
CTGGCTCGAG ATTGCTTGGA TAAATCCCAC CAGGGCCTCA TCACCTCACG ATACTTCCTT 
GAATTACAGC ACAAATTAGA TAAGTTGCTA CAGGAGGCTC ATGATCGTTC AGAAAGTGGA 
GAATTGGCAT TTATTAAACA ACTAGTTCGA AAGATCCTAA TTGTTATTGC CCGCCCTGCT 
CGGTTATTAG AGTGCCTGGA ATTTGATCCG GAAGAATTTT ACTACCTATT GGAAGCAGCA 
GAAGGCCATG CCAAAGAAGG ACAGGGTATT AAAACCGACA TTCCCAGGTA CATCATTAGC 
CAACTGGGAC TCAATAAGGA TCCCTTGGAA GAAATGGCTC ATTTGGGAAA CTACGATAGT 
GGGACAGCAG AAACACCAGA AACAGATGAA TCAGTGAGTA GCTCTAATGC CTCCCTGAAA 
CTTCGAAGGA AACCTCGGGA AAGTGATTTT GAAACGATTA AATTGATTAG CAATGGAGCC 
TATGGGGCAG TCTACTTTGT TCGGCATAAA GAATCCCGGC AGAGGTTTGC CATGAAGAAG 
ATTAATAAAC AGAACCTCAT CCTTCGAAAC CAGATCCAGC AGGCCTTTGT GGAGCGGGAT 
ATCCTGACTT TTGCAGAAAA CCCCTTTGTT GTCAGCATGT ATTGCTCCTT TGAAACAAGG 
CGCCACTTGT GCATGGTCAT GGAATATGTG GAAGGGGGAG ACTGTGCTAC TTTAATGAAA 
AACATGGGTC CTCTCCCTGT TGATATGGCC AGAATGTACT TTGCTGAGAC GGTCTTGGCC 
TTGGAATATT TACATAATTA TGGAATTGTA CACAGGGATT TGAAACCAGA CAACTTGTTG 
GTTACCTCCA TGGGGCACAT AAAGCTGACA GATTTTGGAT TATCTAAGGT GGGACTAATG 
AGCATGACTA CCAACCTTTA CGAGGGTCAT ATTGAGAAGG ATGCTAGAGA GTTCCTGGAT 
AAACAGGTCT GTGGCACACC TGAATACATT GCACCAGAAG TGATTCTGAG GCAGGGTTAT 
GGAAAGCCGG TGGACTGGTG GGCCATGGGG ATTATCCTCT ATGAATTTCT GGTTGGATGC 
GTGCCATTCT TTGGGGATAC TCCAGAGGAG CTATTTGGAC AAGTCATCAG TGATGAGATC 
AACTGGCCTG AGAAGGATGA GGCACCCCCA CCTGATGCCC AGGATCTGAT TACCTTACTC 
CTCAGGCAGA ATCCCCTGGA GAGGCTGGGA ACAGGTGGTG CATATGAAGT CAAACAGCAT 
CGATTCTTCC GTTCTTTAGA CTGGAACAGT TTGCTGAGAC AGAAGGCAGA ATTTATTCCC 
CAACTGGAAT CTGAGGATGA CACAAGTTAT TTTGATACTC GGTCTGAGAA GTATCATCAT 
ATGGAAACGG AGGAAGAAGA TGACACAAAT GATGAAGACT TTAATGTGGA AATAAGGCAG 
TTTTCTTCAT GTTCACACAG GTTTTCAAAA GTTTTCAGCA GTATAGATCG AATCACTCAG 
AATTCAGCAG AAGAGAAGGA AGACTCTGTG GACAAAACCA AAAGCACCAC CTTGCCATCC 
ACAGAAACAC TGAGCTGGAG TTCAGAATAT TCTGAAATGC AACAGCTATC AACATCCAAC 
TCTTCAGATA CTGAAAGCAA CAGACATAAA CTCAGTTCTG GCCTACTTCC CAAACTGGCT 
ATTTCAACAG AGGGAGAGCA AGATGAAGCT GCCTCCTGCC CTGGAGACCC CCATGAGGAG 
CCAGGAAAGC CAGCCCTTCC TCCTGAAGAG TGTGCCCAGG AGGAGCCTGA GGTCACCACC 
CCAGCCAGCA CCATCAGCAG CTCCACCCTG TCAGTTGGCA GTTTTTCAGA GCACTTGGAT 
CAGATAAATG GACGAAGCGA GTGTGTGGAC AGTACAGATA ATTCCTCAAA GCCATCCAGT 
GAACCCGCTT CTCACATGGC TCGGCAGCGA TTAGAAAGCA CAGAAAAAAA GAAAATCTCG 
GGGAAAGTCA CAAAGTCCCT CTCTGCCAGT GCTCTTTCCC TCATGATCCC AGGAGATATG 
TTTGCTGTTT CCCCTCTGGG AAGTCCAATG TCTCCCCATT CCCTGTCCTC GGACCCTTCT 
TCTTCACGAG ATTCCTCTCC CAGCCGAGAT TCCTCAGCAG CTTCTGCCAG TCCACATCAG 
CCGATTGTGA TCCACAGTTC GGGGAAGAAC TACGGCTTTA CCATCCGAGC CATCCGGGTG 
TATGTGGGAG ACAGTGACAT CTATACAGTG CACCATATCG TCTGGAATGT AGAAGAAGGA 
AGTCCGGCAT GCCAGGCAGG ACTGAAGGCT GGAGATCTTA TCACTCACAT CAATGGAGAA 
CCAGTGCATG GACTTGTCCA CACAGAAGTT ATAGAACTCC TACTGAAGAG TGGGAATAAG 
GTGTCAATCA CTACTACCCC ATTTGAAAAC ACATCAATCA AAACTGGACC AGCCAGGAGA 
AACAGCTATA AGAGCCGGAT GGTGAGGCGG AGCAAGAAAT CCAAGAAGAA AGAAAGTCTC 
GAAAGGAGGA GATCTCTTTT CAAAAAGCTA GCCAAGCAGC CTTCTCCTTT ACTCCACACC 
AGCCGAAGTT TCTCCTGCTT GAACAGATCC CTGTCATCGG GTGAGAGCCT CCCAGGTTCC 
CCCACTCATA GCTTGTCTCC CCGGTCTCCA ACACCAAGCT ACCGCTCCAC CCCTGACTTC 
CCATCTGGTA CTAATTCCTC CCAGAGCAGC TCCCCTAGTT CTAGTGCCCC CAATTCCCCA 
GCAGGGTCCG GGCACATCCG GCCCAGCACT CTCCACGGTC TTGCACCCAA ACTCGGCGGG 
CAGCGGTACC GGTCCGGAAG GCGAAAGTCC GCCGGCAACA TCCCACTGTC CCCGCTGGCC 
CGGACGCCCT CTCCAACCCC GCAACCCACC TCCCCGCAGC GGTCACCATC CCCTCTTCTG 
GGACACTCAC TGGGCAATTC CAAGATCGCG CAAGCCTTTC CCAGCAAGAT GCACTCCCCG 
CCCACCATCG TCAGACACAT CGTGAGGCCC AAGAGTGCGG AGCCCCCCAG GTCCCCGCTG 
CTCAAGCGCG TGCAGTCCGA GGAGAAGCTG TCGCCCTCTT ACGGCAGTGA CAAGAAGCAC 
CTGTGCTCCC GCAAGCACAG CCTGGAGGTG ACCCAAGAGG AGGTGCAGCG GGAGCAGTCC 
CAGCGGGAGG CGCCGCTGCA GAGCCTGGAT GAGAACGTGT GCGACGTGCC GCCGCTCAGC 
CGCGCCCGGC CAGTGGAGCA AGGCTGCCTG AAACGCCCAG TCTCCCGGAA GGTGGGCCGC 
CAGGAGTCTG TGGACGACCT GGACCGCGAC AAGCTGAAGG CCAAGGTGGT GGTGAAGAAA 
GCAGACGGCT TCCCAGAGAA ACAGGAATCC CACCAGAAAT CCCATGGACC CGGGAGTGAT 
TTGGAAAACT TTGCTCTGTT TAAGCTGGAA GAGAGAGAGA AGAAAGTCTA TCCGAAGGCT 
GTGGAAAGGT CAAGTACTTT TGAAAACAAA GCGTCTATGC AGGAGGCGCC ACCGCTGGGC 
AGCCTGCTGA AGGATGCTCT TCACAAGCAG GCCAGCGTGC GCGCCAGCGA GGGTGCGATG 
TCGGATGGCC GGGTGCCTGC GGAGCACCGC CAGGGTGGCG GGGACTTCAG ACGGGCCCCC 
GCTCCTGGCA CCCTCCAGGA TGGTCTCTGC CACTCCCTCG ACAGGGGCAT CTCTGGGAAG 
GGGGAAGGCA CGGAGAAGTC CTCCCAGGCC AAGGAGCTTC TCCGATGTGA AAAGTTAGAC 
AGCAAGCTGG CCAACATCGA TTACCTCCGA AAGAAAATGT CACTTGAGGA CAAAGAGGAC 
AACCTCTGCC CTGTGCTGAA GCCCAAGATG ACAGCTGGCT CCCACGAATG CCTGCCAGGG 
AACCCAGTCC GACCCACGGG TGGGCAGCAG GAGCCCCCGC CGGCTTCTGA GAGCCGAGCT 
TTTGTCAGCA GCACCCATGC AGCTCAGATG AGTGCCGTCT CTTTTGTTCC CCTCAAGGCC 
TTAACAGGCC GGGTGGACAG TGGAACGGAG AAGCCTGGCT TGGTTGCTCC TGAGTCCCCT 
GTTAGGAAGA GCCCCTCCGA GTATAAGCTG GAAGGTAGGT CTGTCTCATG CCTGAAGCCG 
ATCGAGGGCA CTCTGGACAT TGCTCTCCTG TCCGGACCTC AGGCCTCCAA GACAGAACTG 
CCTTCCCCAG AGTCTGCACA GAGCCCCAGC CCAAGTGGTG ACGTGAGGGC CTCTGTGCCA 
CCAGTTCTCC CCAGCAGCAG TGGGAAAAAG AACGATACCA CCAGTGCAAG AGAGCTTTCT 
CCTTCCAGCT TAAAGATGAA TAAATCCTAC CTGCTGGAGC CTTGGTTCCT GCCCCCCAGC 
CGAGGTCTCC AGAATTCACC AGCAGTTTCC CTGCCTGACC CAGAGTTCAA GAGGGACAGG 
AAAGGTCCCC ATCCTACTGC CAGGAGCCCT GGAACAGTCA TGGAAAGCAA TCCCCAACAG 
AGAGAGGGCA GCTCCCCTAA ACACCAAGAC CACACCACTG ACCCCAAGCT TCTGACCTGC 
CTGGGGCAGA ACCTCCACAG CCCTGACCTG GCCAGGCCAC GCTGCCCGCT CCCACCTGAA 
GCTTCCCCCT CAAGGGAGAA GCCAGGCCTG AGGGAATCGT CTGAAAGAGG CCCTCCCACA 
GCCAGAAGCG AGCGCTCTGC TGCGAGGGCT GACACATGCA GAGAGCCCTC CATGGAACTG 
TGCTTTCCAG AAACTGCGAA AACCAGTGAC AACTCCAAAA ATCTCCTCTC TGTGGGAAGG 
ACCCACCCAG ATTTCTATAC ACAGACCCAG GCCATGGAGA AAGCATGGGC GCCGGGTGGG 
AAAACGAACC ACAAAGATGG CCCAGGTGAG GCGAGGCCCC CGCCCAGAGA CAACTCCTCT 
CTGCACTCAG CTGGAATTCC CTGTGAGAAG GAGCTGGGCA AGGTGAGGCG TGGCGTGGAA 
CCCAAGCCCG AAGCGCTTCT TGCCAGGCGG TCTCTGCAGC CACCTGGAAT TGAGAGTGAG 
AAGAGTGAAA AGCTCTCCAG TTTCCCATCT TTGCAGAAAG ATGGTGCCAA GGAACCTGAA 
AGGAAGGAGC AGCCTCTACA AAGGCATCCC AGCAGCATCC CTCCGCCCCC TCTGACGGCC 
AAAGACCTGT CCAGCCCGGC TGCCAGGCAG CATTGCAGTT CCCCAAGCCA CGCTTCTGGC 
AGAGAGCCGG GGGCCAAGCC CAGCACTGCA GAGCCCAGCT CGAGCCCCCA GGACCCTCCC 
AAGCCTGTTG CTGCGCACAG TGAAAGCAGC AGCCACAAGC CCCGGCCTGG CCCTGACCCG 
GGCCCTCCAA AGACTAAGCA CCCCGACCGG TCCCTCTCCT CTCAGAAACC AAGTGTCGGG 
GCCACAAAGG GCAAAGAGCC TGCCACTCAG TCCCTCGGTG GCTCTAGCAG AGAGGGGAAG 
GGCCACAGTA AGAGTGGGCC GGATGTGTTT CCTGCTACCC CAGGCTCCCA GAACAAAGCC 
AGCGATGGGA TTGGCCAGGG AGAAGGTGGG CCCTCTGTCC CACTGCACAC TGACAGGGCT 
CCTCTAGACG CCAAGCCACA ACCCACCAGT GGTGGGCGGC CCCTGGAGGT GCTGGAGAAG 
CCTGTGCATT TGCCAAGGCC GGGACACCCA GGGCCTAGTG AGCCAGCGGA CCAGAAACTG 
TCCGCTGTTG GTGAAAAGCA AACCCTGTCT CCAAAGCACC CCAAACCATC CACTGTGAAA 
GATTGCCCCA CCCTGTGCAA ACAGACAGAC AACAGACAGA CAGACAAAAG CCCGAGTCAG 
CCGGCCGCCA ACACCGACAG AAGGGCGGAA GGGAAGAAAT GCACTGAAGC ACTTTATGCT 
CCAGCAGAGG GCGACAAGCT CGAGGCCGGC CTTTCCTTTG TGCATAGCGA GAACCGGTTG 
AAAGGCGCGG AGCGGCCAGC CGCGGGGGTG GGGAAGGGCT TCCCTGAGGC CAGAGGGAAA 
GGGCCCGGTC CCCAGAAGCC ACCGACGGAG GCAGACAAGC CCAATGGCAT GAAACGGTCC 
CCCTCAGCCA CTGGGCAGAG TTCTTTCCGA TCCACGGCCC TCCCGGAAAA GTCTCTGAGC 
TGCTCCTCCA GCTTCCCTGA AACCAGGGCC GGAGTTAGAG AGGCCTCTGC AGCCAGCAGC 
GACACCTCTT CTGCCAAGGC CGCCGGGGGC ATGCTGGAGC TTCCAGCCCC CAGCAACAGG 
GACCATAGGA AGGCTCAGCC TGCCGGGGAG GGCCGAACCC ACATGACAAA GAGTGACTCC 
CTGCCCTCCT TCCGGGTCTC CACCCTGCCT CTGGAGTCAC ACCACCCCGA CCCAAACACC 
ATGGGCGGGG CCAGCCACCG GGACAGGGCT CTCTCGGTGA CTGCCACCGT AGGGGAAACC 
AAAGGGAAGG ACCCTGCCCC AGCCCAGCCT CCCCCAGCTA GGAAACAGAA CGTGGGCAGA 
GACGTGACCA AGCCATCCCC AGCCCCAAAC ACTGACCGCC CCATCTCTCT TTCTAATGAG 
AAGGACTTTG TGGTACGGCA GAGGCGGGGG AAAGAGAGTT TGCGTAGCAG CCCTCACAAA 
AAGGCCTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( NM_001164664.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001158136.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 3, mRNA.

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CloneID	OHu21594
Clone ID Related Accession (Same CDS sequence)	NM_001164664.1 , NM_001164664.2
Accession Version	NM_001164664.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC092373.2, AC016634.7, AC008872.6, AC008920.7 and AC044799.5. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001164664.1. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2155371 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTTTGCCG AACAAGCAAC CGGAAAAGCT TAATAGGCAA TGGGCAGTCA 
CCAGCATTGC CTCGACCACA CTCACCTCTC TCTGCTCATG CAGGAAATAG CCCTCAAGAT 
AGTCCAAGAA ATTTCTCCCC CAGTGCCTCA GCCCATTTTT CATTTGCACG GAGGACTGAT 
GGACGCCGCT GGTCGTTGGC TTCTCTCCCT TCCTCTGGCT ATGGGACAAA CACACCCAGC 
TCTACGGTCT CTTCATCCTG TTCCTCCCAG GAGAAGTTGC ATCAGTTACC ATACCAACCA 
ACACCAGACG AGTTACACTT CTTATCAAAA CATTTCTGTA CCACCGAAAG CATCGCCACT 
GAGAACAGAT GCAGGAACAC GCCGATGCGC CCCCGTTCCC GAAGTCTGAG CCCTGGACGT 
TCTCCCGCCT GCTGTGACCA TGAAATAATT ATGATGAACC ATGTCTACAA AGAAAGGTTC 
CCAAAGGCTA CAGCTCAGAT GGAAGAACGT CTAAAGGAAA TTATCACCAG CTACTCTCCT 
GACAACGTTC TACCCTTAGC AGATGGAGTG CTTAGTTTCA CTCACCACCA GATTATTGAA 
CTGGCTCGAG ATTGCTTGGA TAAATCCCAC CAGGGCCTCA TCACCTCACG ATACTTCCTT 
GAATTACAGC ACAAATTAGA TAAGTTGCTA CAGGAGGCTC ATGATCGTTC AGAAAGTGGA 
GAATTGGCAT TTATTAAACA ACTAGTTCGA AAGATCCTAA TTGTTATTGC CCGCCCTGCT 
CGGTTATTAG AGTGCCTGGA ATTTGATCCG GAAGAATTTT ACTACCTATT GGAAGCAGCA 
GAAGGCCATG CCAAAGAAGG ACAGGGTATT AAAACCGACA TTCCCAGGTA CATCATTAGC 
CAACTGGGAC TCAATAAGGA TCCCTTGGAA GAAATGGCTC ATTTGGGAAA CTACGATAGT 
GGGACAGCAG AAACACCAGA AACAGATGAA TCAGTGAGTA GCTCTAATGC CTCCCTGAAA 
CTTCGAAGGA AACCTCGGGA AAGTGATTTT GAAACGATTA AATTGATTAG CAATGGAGCC 
TATGGGGCAG TCTACTTTGT TCGGCATAAA GAATCCCGGC AGAGGTTTGC CATGAAGAAG 
ATTAATAAAC AGAACCTCAT CCTTCGAAAC CAGATCCAGC AGGCCTTTGT GGAGCGGGAT 
ATCCTGACTT TTGCAGAAAA CCCCTTTGTT GTCAGCATGT ATTGCTCCTT TGAAACAAGG 
CGCCACTTGT GCATGGTCAT GGAATATGTG GAAGGGGGAG ACTGTGCTAC TTTAATGAAA 
AACATGGGTC CTCTCCCTGT TGATATGGCC AGAATGTACT TTGCTGAGAC GGTCTTGGCC 
TTGGAATATT TACATAATTA TGGAATTGTA CACAGGGATT TGAAACCAGA CAACTTGTTG 
GTTACCTCCA TGGGGCACAT AAAGCTGACA GATTTTGGAT TATCTAAGGT GGGACTAATG 
AGCATGACTA CCAACCTTTA CGAGGGTCAT ATTGAGAAGG ATGCTAGAGA GTTCCTGGAT 
AAACAGGTCT GTGGCACACC TGAATACATT GCACCAGAAG TGATTCTGAG GCAGGGTTAT 
GGAAAGCCGG TGGACTGGTG GGCCATGGGG ATTATCCTCT ATGAATTTCT GGTTGGATGC 
GTGCCATTCT TTGGGGATAC TCCAGAGGAG CTATTTGGAC AAGTCATCAG TGATGAGATC 
AACTGGCCTG AGAAGGATGA GGCACCCCCA CCTGATGCCC AGGATCTGAT TACCTTACTC 
CTCAGGCAGA ATCCCCTGGA GAGGCTGGGA ACAGGTGGTG CATATGAAGT CAAACAGCAT 
CGATTCTTCC GTTCTTTAGA CTGGAACAGT TTGCTGAGAC AGAAGGCAGA ATTTATTCCC 
CAACTGGAAT CTGAGGATGA CACAAGTTAT TTTGATACTC GGTCTGAGAA GTATCATCAT 
ATGGAAACGG AGGAAGAAGA TGACACAAAT GATGAAGACT TTAATGTGGA AATAAGGCAG 
TTTTCTTCAT GTTCACACAG GTTTTCAAAA GTTTTCAGCA GTATAGATCG AATCACTCAG 
AATTCAGCAG AAGAGAAGGA AGACTCTGTG GACAAAACCA AAAGCACCAC CTTGCCATCC 
ACAGAAACAC TGAGCTGGAG TTCAGAATAT TCTGAAATGC AACAGCTATC AACATCCAAC 
TCTTCAGATA CTGAAAGCAA CAGACATAAA CTCAGTTCTG GCCTACTTCC CAAACTGGCT 
ATTTCAACAG AGGGAGAGCA AGATGAAGCT GCCTCCTGCC CTGGAGACCC CCATGAGGAG 
CCAGGAAAGC CAGCCCTTCC TCCTGAAGAG TGTGCCCAGG AGGAGCCTGA GGTCACCACC 
CCAGCCAGCA CCATCAGCAG CTCCACCCTG TCAGTTGGCA GTTTTTCAGA GCACTTGGAT 
CAGATAAATG GACGAAGCGA GTGTGTGGAC AGTACAGATA ATTCCTCAAA GCCATCCAGT 
GAACCCGCTT CTCACATGGC TCGGCAGCGA TTAGAAAGCA CAGAAAAAAA GAAAATCTCG 
GGGAAAGTCA CAAAGTCCCT CTCTGCCAGT GCTCTTTCCC TCATGATCCC AGGAGATATG 
TTTGCTGTTT CCCCTCTGGG AAGTCCAATG TCTCCCCATT CCCTGTCCTC GGACCCTTCT 
TCTTCACGAG ATTCCTCTCC CAGCCGAGAT TCCTCAGCAG CTTCTGCCAG TCCACATCAG 
CCGATTGTGA TCCACAGTTC GGGGAAGAAC TACGGCTTTA CCATCCGAGC CATCCGGGTG 
TATGTGGGAG ACAGTGACAT CTATACAGTG CACCATATCG TCTGGAATGT AGAAGAAGGA 
AGTCCGGCAT GCCAGGCAGG ACTGAAGGCT GGAGATCTTA TCACTCACAT CAATGGAGAA 
CCAGTGCATG GACTTGTCCA CACAGAAGTT ATAGAACTCC TACTGAAGAG TGGGAATAAG 
GTGTCAATCA CTACTACCCC ATTTGAAAAC ACATCAATCA AAACTGGACC AGCCAGGAGA 
AACAGCTATA AGAGCCGGAT GGTGAGGCGG AGCAAGAAAT CCAAGAAGAA AGAAAGTCTC 
GAAAGGAGGA GATCTCTTTT CAAAAAGCTA GCCAAGCAGC CTTCTCCTTT ACTCCACACC 
AGCCGAAGTT TCTCCTGCTT GAACAGATCC CTGTCATCGG GTGAGAGCCT CCCAGGTTCC 
CCCACTCATA GCTTGTCTCC CCGGTCTCCA ACACCAAGCT ACCGCTCCAC CCCTGACTTC 
CCATCTGGTA CTAATTCCTC CCAGAGCAGC TCCCCTAGTT CTAGTGCCCC CAATTCCCCA 
GCAGGGTCCG GGCACATCCG GCCCAGCACT CTCCACGGTC TTGCACCCAA ACTCGGCGGG 
CAGCGGTACC GGTCCGGAAG GCGAAAGTCC GCCGGCAACA TCCCACTGTC CCCGCTGGCC 
CGGACGCCCT CTCCAACCCC GCAACCCACC TCCCCGCAGC GGTCACCATC CCCTCTTCTG 
GGACACTCAC TGGGCAATTC CAAGATCGCG CAAGCCTTTC CCAGCAAGAT GCACTCCCCG 
CCCACCATCG TCAGACACAT CGTGAGGCCC AAGAGTGCGG AGCCCCCCAG GTCCCCGCTG 
CTCAAGCGCG TGCAGTCCGA GGAGAAGCTG TCGCCCTCTT ACGGCAGTGA CAAGAAGCAC 
CTGTGCTCCC GCAAGCACAG CCTGGAGGTG ACCCAAGAGG AGGTGCAGCG GGAGCAGTCC 
CAGCGGGAGG CGCCGCTGCA GAGCCTGGAT GAGAACGTGT GCGACGTGCC GCCGCTCAGC 
CGCGCCCGGC CAGTGGAGCA AGGCTGCCTG AAACGCCCAG TCTCCCGGAA GGTGGGCCGC 
CAGGAGTCTG TGGACGACCT GGACCGCGAC AAGCTGAAGG CCAAGGTGGT GGTGAAGAAA 
GCAGACGGCT TCCCAGAGAA ACAGGAATCC CACCAGAAAT CCCATGGACC CGGGAGTGAT 
TTGGAAAACT TTGCTCTGTT TAAGCTGGAA GAGAGAGAGA AGAAAGTCTA TCCGAAGGCT 
GTGGAAAGGT CAAGTACTTT TGAAAACAAA GCGTCTATGC AGGAGGCGCC ACCGCTGGGC 
AGCCTGCTGA AGGATGCTCT TCACAAGCAG GCCAGCGTGC GCGCCAGCGA GGGTGCGATG 
TCGGATGGCC GGGTGCCTGC GGAGCACCGC CAGGGTGGCG GGGACTTCAG ACGGGCCCCC 
GCTCCTGGCA CCCTCCAGGA TGGTCTCTGC CACTCCCTCG ACAGGGGCAT CTCTGGGAAG 
GGGGAAGGCA CGGAGAAGTC CTCCCAGGCC AAGGAGCTTC TCCGATGTGA AAAGTTAGAC 
AGCAAGCTGG CCAACATCGA TTACCTCCGA AAGAAAATGT CACTTGAGGA CAAAGAGGAC 
AACCTCTGCC CTGTGCTGAA GCCCAAGATG ACAGCTGGCT CCCACGAATG CCTGCCAGGG 
AACCCAGTCC GACCCACGGG TGGGCAGCAG GAGCCCCCGC CGGCTTCTGA GAGCCGAGCT 
TTTGTCAGCA GCACCCATGC AGCTCAGATG AGTGCCGTCT CTTTTGTTCC CCTCAAGGCC 
TTAACAGGCC GGGTGGACAG TGGAACGGAG AAGCCTGGCT TGGTTGCTCC TGAGTCCCCT 
GTTAGGAAGA GCCCCTCCGA GTATAAGCTG GAAGGTAGGT CTGTCTCATG CCTGAAGCCG 
ATCGAGGGCA CTCTGGACAT TGCTCTCCTG TCCGGACCTC AGGCCTCCAA GACAGAACTG 
CCTTCCCCAG AGTCTGCACA GAGCCCCAGC CCAAGTGGTG ACGTGAGGGC CTCTGTGCCA 
CCAGTTCTCC CCAGCAGCAG TGGGAAAAAG AACGATACCA CCAGTGCAAG AGAGCTTTCT 
CCTTCCAGCT TAAAGATGAA TAAATCCTAC CTGCTGGAGC CTTGGTTCCT GCCCCCCAGC 
CGAGGTCTCC AGAATTCACC AGCAGTTTCC CTGCCTGACC CAGAGTTCAA GAGGGACAGG 
AAAGGTCCCC ATCCTACTGC CAGGAGCCCT GGAACAGTCA TGGAAAGCAA TCCCCAACAG 
AGAGAGGGCA GCTCCCCTAA ACACCAAGAC CACACCACTG ACCCCAAGCT TCTGACCTGC 
CTGGGGCAGA ACCTCCACAG CCCTGACCTG GCCAGGCCAC GCTGCCCGCT CCCACCTGAA 
GCTTCCCCCT CAAGGGAGAA GCCAGGCCTG AGGGAATCGT CTGAAAGAGG CCCTCCCACA 
GCCAGAAGCG AGCGCTCTGC TGCGAGGGCT GACACATGCA GAGAGCCCTC CATGGAACTG 
TGCTTTCCAG AAACTGCGAA AACCAGTGAC AACTCCAAAA ATCTCCTCTC TGTGGGAAGG 
ACCCACCCAG ATTTCTATAC ACAGACCCAG GCCATGGAGA AAGCATGGGC GCCGGGTGGG 
AAAACGAACC ACAAAGATGG CCCAGGTGAG GCGAGGCCCC CGCCCAGAGA CAACTCCTCT 
CTGCACTCAG CTGGAATTCC CTGTGAGAAG GAGCTGGGCA AGGTGAGGCG TGGCGTGGAA 
CCCAAGCCCG AAGCGCTTCT TGCCAGGCGG TCTCTGCAGC CACCTGGAAT TGAGAGTGAG 
AAGAGTGAAA AGCTCTCCAG TTTCCCATCT TTGCAGAAAG ATGGTGCCAA GGAACCTGAA 
AGGAAGGAGC AGCCTCTACA AAGGCATCCC AGCAGCATCC CTCCGCCCCC TCTGACGGCC 
AAAGACCTGT CCAGCCCGGC TGCCAGGCAG CATTGCAGTT CCCCAAGCCA CGCTTCTGGC 
AGAGAGCCGG GGGCCAAGCC CAGCACTGCA GAGCCCAGCT CGAGCCCCCA GGACCCTCCC 
AAGCCTGTTG CTGCGCACAG TGAAAGCAGC AGCCACAAGC CCCGGCCTGG CCCTGACCCG 
GGCCCTCCAA AGACTAAGCA CCCCGACCGG TCCCTCTCCT CTCAGAAACC AAGTGTCGGG 
GCCACAAAGG GCAAAGAGCC TGCCACTCAG TCCCTCGGTG GCTCTAGCAG AGAGGGGAAG 
GGCCACAGTA AGAGTGGGCC GGATGTGTTT CCTGCTACCC CAGGCTCCCA GAACAAAGCC 
AGCGATGGGA TTGGCCAGGG AGAAGGTGGG CCCTCTGTCC CACTGCACAC TGACAGGGCT 
CCTCTAGACG CCAAGCCACA ACCCACCAGT GGTGGGCGGC CCCTGGAGGT GCTGGAGAAG 
CCTGTGCATT TGCCAAGGCC GGGACACCCA GGGCCTAGTG AGCCAGCGGA CCAGAAACTG 
TCCGCTGTTG GTGAAAAGCA AACCCTGTCT CCAAAGCACC CCAAACCATC CACTGTGAAA 
GATTGCCCCA CCCTGTGCAA ACAGACAGAC AACAGACAGA CAGACAAAAG CCCGAGTCAG 
CCGGCCGCCA ACACCGACAG AAGGGCGGAA GGGAAGAAAT GCACTGAAGC ACTTTATGCT 
CCAGCAGAGG GCGACAAGCT CGAGGCCGGC CTTTCCTTTG TGCATAGCGA GAACCGGTTG 
AAAGGCGCGG AGCGGCCAGC CGCGGGGGTG GGGAAGGGCT TCCCTGAGGC CAGAGGGAAA 
GGGCCCGGTC CCCAGAAGCC ACCGACGGAG GCAGACAAGC CCAATGGCAT GAAACGGTCC 
CCCTCAGCCA CTGGGCAGAG TTCTTTCCGA TCCACGGCCC TCCCGGAAAA GTCTCTGAGC 
TGCTCCTCCA GCTTCCCTGA AACCAGGGCC GGAGTTAGAG AGGCCTCTGC AGCCAGCAGC 
GACACCTCTT CTGCCAAGGC CGCCGGGGGC ATGCTGGAGC TTCCAGCCCC CAGCAACAGG 
GACCATAGGA AGGCTCAGCC TGCCGGGGAG GGCCGAACCC ACATGACAAA GAGTGACTCC 
CTGCCCTCCT TCCGGGTCTC CACCCTGCCT CTGGAGTCAC ACCACCCCGA CCCAAACACC 
ATGGGCGGGG CCAGCCACCG GGACAGGGCT CTCTCGGTGA CTGCCACCGT AGGGGAAACC 
AAAGGGAAGG ACCCTGCCCC AGCCCAGCCT CCCCCAGCTA GGAAACAGAA CGTGGGCAGA 
GACGTGACCA AGCCATCCCC AGCCCCAAAC ACTGACCGCC CCATCTCTCT TTCTAATGAG 
AAGGACTTTG TGGTACGGCA GAGGCGGGGG AAAGAGAGTT TGCGTAGCAG CCCTCACAAA 
AAGGCCTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001158136.1
CDS	264..8135
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLKELSLPRRGSFCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	NM_001164664.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001164664.2

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGAAGGAGCT GAGTCTCCCC 
AGAAGAGGAA GTTTTTGCCG AACAAGCAAC CGGAAAAGCT TAATAGGCAA TGGGCAGTCA 
CCAGCATTGC CTCGACCACA CTCACCTCTC TCTGCTCATG CAGGAAATAG CCCTCAAGAT 
AGTCCAAGAA ATTTCTCCCC CAGTGCCTCA GCCCATTTTT CATTTGCACG GAGGACTGAT 
GGACGCCGCT GGTCGTTGGC TTCTCTCCCT TCCTCTGGCT ATGGGACAAA CACACCCAGC 
TCTACGGTCT CTTCATCCTG TTCCTCCCAG GAGAAGTTGC ATCAGTTACC ATACCAACCA 
ACACCAGACG AGTTACACTT CTTATCAAAA CATTTCTGTA CCACCGAAAG CATCGCCACT 
GAGAACAGAT GCAGGAACAC GCCGATGCGC CCCCGTTCCC GAAGTCTGAG CCCTGGACGT 
TCTCCCGCCT GCTGTGACCA TGAAATAATT ATGATGAACC ATGTCTACAA AGAAAGGTTC 
CCAAAGGCTA CAGCTCAGAT GGAAGAACGT CTAAAGGAAA TTATCACCAG CTACTCTCCT 
GACAACGTTC TACCCTTAGC AGATGGAGTG CTTAGTTTCA CTCACCACCA GATTATTGAA 
CTGGCTCGAG ATTGCTTGGA TAAATCCCAC CAGGGCCTCA TCACCTCACG ATACTTCCTT 
GAATTACAGC ACAAATTAGA TAAGTTGCTA CAGGAGGCTC ATGATCGTTC AGAAAGTGGA 
GAATTGGCAT TTATTAAACA ACTAGTTCGA AAGATCCTAA TTGTTATTGC CCGCCCTGCT 
CGGTTATTAG AGTGCCTGGA ATTTGATCCG GAAGAATTTT ACTACCTATT GGAAGCAGCA 
GAAGGCCATG CCAAAGAAGG ACAGGGTATT AAAACCGACA TTCCCAGGTA CATCATTAGC 
CAACTGGGAC TCAATAAGGA TCCCTTGGAA GAAATGGCTC ATTTGGGAAA CTACGATAGT 
GGGACAGCAG AAACACCAGA AACAGATGAA TCAGTGAGTA GCTCTAATGC CTCCCTGAAA 
CTTCGAAGGA AACCTCGGGA AAGTGATTTT GAAACGATTA AATTGATTAG CAATGGAGCC 
TATGGGGCAG TCTACTTTGT TCGGCATAAA GAATCCCGGC AGAGGTTTGC CATGAAGAAG 
ATTAATAAAC AGAACCTCAT CCTTCGAAAC CAGATCCAGC AGGCCTTTGT GGAGCGGGAT 
ATCCTGACTT TTGCAGAAAA CCCCTTTGTT GTCAGCATGT ATTGCTCCTT TGAAACAAGG 
CGCCACTTGT GCATGGTCAT GGAATATGTG GAAGGGGGAG ACTGTGCTAC TTTAATGAAA 
AACATGGGTC CTCTCCCTGT TGATATGGCC AGAATGTACT TTGCTGAGAC GGTCTTGGCC 
TTGGAATATT TACATAATTA TGGAATTGTA CACAGGGATT TGAAACCAGA CAACTTGTTG 
GTTACCTCCA TGGGGCACAT AAAGCTGACA GATTTTGGAT TATCTAAGGT GGGACTAATG 
AGCATGACTA CCAACCTTTA CGAGGGTCAT ATTGAGAAGG ATGCTAGAGA GTTCCTGGAT 
AAACAGGTCT GTGGCACACC TGAATACATT GCACCAGAAG TGATTCTGAG GCAGGGTTAT 
GGAAAGCCGG TGGACTGGTG GGCCATGGGG ATTATCCTCT ATGAATTTCT GGTTGGATGC 
GTGCCATTCT TTGGGGATAC TCCAGAGGAG CTATTTGGAC AAGTCATCAG TGATGAGATC 
AACTGGCCTG AGAAGGATGA GGCACCCCCA CCTGATGCCC AGGATCTGAT TACCTTACTC 
CTCAGGCAGA ATCCCCTGGA GAGGCTGGGA ACAGGTGGTG CATATGAAGT CAAACAGCAT 
CGATTCTTCC GTTCTTTAGA CTGGAACAGT TTGCTGAGAC AGAAGGCAGA ATTTATTCCC 
CAACTGGAAT CTGAGGATGA CACAAGTTAT TTTGATACTC GGTCTGAGAA GTATCATCAT 
ATGGAAACGG AGGAAGAAGA TGACACAAAT GATGAAGACT TTAATGTGGA AATAAGGCAG 
TTTTCTTCAT GTTCACACAG GTTTTCAAAA GTTTTCAGCA GTATAGATCG AATCACTCAG 
AATTCAGCAG AAGAGAAGGA AGACTCTGTG GACAAAACCA AAAGCACCAC CTTGCCATCC 
ACAGAAACAC TGAGCTGGAG TTCAGAATAT TCTGAAATGC AACAGCTATC AACATCCAAC 
TCTTCAGATA CTGAAAGCAA CAGACATAAA CTCAGTTCTG GCCTACTTCC CAAACTGGCT 
ATTTCAACAG AGGGAGAGCA AGATGAAGCT GCCTCCTGCC CTGGAGACCC CCATGAGGAG 
CCAGGAAAGC CAGCCCTTCC TCCTGAAGAG TGTGCCCAGG AGGAGCCTGA GGTCACCACC 
CCAGCCAGCA CCATCAGCAG CTCCACCCTG TCAGTTGGCA GTTTTTCAGA GCACTTGGAT 
CAGATAAATG GACGAAGCGA GTGTGTGGAC AGTACAGATA ATTCCTCAAA GCCATCCAGT 
GAACCCGCTT CTCACATGGC TCGGCAGCGA TTAGAAAGCA CAGAAAAAAA GAAAATCTCG 
GGGAAAGTCA CAAAGTCCCT CTCTGCCAGT GCTCTTTCCC TCATGATCCC AGGAGATATG 
TTTGCTGTTT CCCCTCTGGG AAGTCCAATG TCTCCCCATT CCCTGTCCTC GGACCCTTCT 
TCTTCACGAG ATTCCTCTCC CAGCCGAGAT TCCTCAGCAG CTTCTGCCAG TCCACATCAG 
CCGATTGTGA TCCACAGTTC GGGGAAGAAC TACGGCTTTA CCATCCGAGC CATCCGGGTG 
TATGTGGGAG ACAGTGACAT CTATACAGTG CACCATATCG TCTGGAATGT AGAAGAAGGA 
AGTCCGGCAT GCCAGGCAGG ACTGAAGGCT GGAGATCTTA TCACTCACAT CAATGGAGAA 
CCAGTGCATG GACTTGTCCA CACAGAAGTT ATAGAACTCC TACTGAAGAG TGGGAATAAG 
GTGTCAATCA CTACTACCCC ATTTGAAAAC ACATCAATCA AAACTGGACC AGCCAGGAGA 
AACAGCTATA AGAGCCGGAT GGTGAGGCGG AGCAAGAAAT CCAAGAAGAA AGAAAGTCTC 
GAAAGGAGGA GATCTCTTTT CAAAAAGCTA GCCAAGCAGC CTTCTCCTTT ACTCCACACC 
AGCCGAAGTT TCTCCTGCTT GAACAGATCC CTGTCATCGG GTGAGAGCCT CCCAGGTTCC 
CCCACTCATA GCTTGTCTCC CCGGTCTCCA ACACCAAGCT ACCGCTCCAC CCCTGACTTC 
CCATCTGGTA CTAATTCCTC CCAGAGCAGC TCCCCTAGTT CTAGTGCCCC CAATTCCCCA 
GCAGGGTCCG GGCACATCCG GCCCAGCACT CTCCACGGTC TTGCACCCAA ACTCGGCGGG 
CAGCGGTACC GGTCCGGAAG GCGAAAGTCC GCCGGCAACA TCCCACTGTC CCCGCTGGCC 
CGGACGCCCT CTCCAACCCC GCAACCCACC TCCCCGCAGC GGTCACCATC CCCTCTTCTG 
GGACACTCAC TGGGCAATTC CAAGATCGCG CAAGCCTTTC CCAGCAAGAT GCACTCCCCG 
CCCACCATCG TCAGACACAT CGTGAGGCCC AAGAGTGCGG AGCCCCCCAG GTCCCCGCTG 
CTCAAGCGCG TGCAGTCCGA GGAGAAGCTG TCGCCCTCTT ACGGCAGTGA CAAGAAGCAC 
CTGTGCTCCC GCAAGCACAG CCTGGAGGTG ACCCAAGAGG AGGTGCAGCG GGAGCAGTCC 
CAGCGGGAGG CGCCGCTGCA GAGCCTGGAT GAGAACGTGT GCGACGTGCC GCCGCTCAGC 
CGCGCCCGGC CAGTGGAGCA AGGCTGCCTG AAACGCCCAG TCTCCCGGAA GGTGGGCCGC 
CAGGAGTCTG TGGACGACCT GGACCGCGAC AAGCTGAAGG CCAAGGTGGT GGTGAAGAAA 
GCAGACGGCT TCCCAGAGAA ACAGGAATCC CACCAGAAAT CCCATGGACC CGGGAGTGAT 
TTGGAAAACT TTGCTCTGTT TAAGCTGGAA GAGAGAGAGA AGAAAGTCTA TCCGAAGGCT 
GTGGAAAGGT CAAGTACTTT TGAAAACAAA GCGTCTATGC AGGAGGCGCC ACCGCTGGGC 
AGCCTGCTGA AGGATGCTCT TCACAAGCAG GCCAGCGTGC GCGCCAGCGA GGGTGCGATG 
TCGGATGGCC GGGTGCCTGC GGAGCACCGC CAGGGTGGCG GGGACTTCAG ACGGGCCCCC 
GCTCCTGGCA CCCTCCAGGA TGGTCTCTGC CACTCCCTCG ACAGGGGCAT CTCTGGGAAG 
GGGGAAGGCA CGGAGAAGTC CTCCCAGGCC AAGGAGCTTC TCCGATGTGA AAAGTTAGAC 
AGCAAGCTGG CCAACATCGA TTACCTCCGA AAGAAAATGT CACTTGAGGA CAAAGAGGAC 
AACCTCTGCC CTGTGCTGAA GCCCAAGATG ACAGCTGGCT CCCACGAATG CCTGCCAGGG 
AACCCAGTCC GACCCACGGG TGGGCAGCAG GAGCCCCCGC CGGCTTCTGA GAGCCGAGCT 
TTTGTCAGCA GCACCCATGC AGCTCAGATG AGTGCCGTCT CTTTTGTTCC CCTCAAGGCC 
TTAACAGGCC GGGTGGACAG TGGAACGGAG AAGCCTGGCT TGGTTGCTCC TGAGTCCCCT 
GTTAGGAAGA GCCCCTCCGA GTATAAGCTG GAAGGTAGGT CTGTCTCATG CCTGAAGCCG 
ATCGAGGGCA CTCTGGACAT TGCTCTCCTG TCCGGACCTC AGGCCTCCAA GACAGAACTG 
CCTTCCCCAG AGTCTGCACA GAGCCCCAGC CCAAGTGGTG ACGTGAGGGC CTCTGTGCCA 
CCAGTTCTCC CCAGCAGCAG TGGGAAAAAG AACGATACCA CCAGTGCAAG AGAGCTTTCT 
CCTTCCAGCT TAAAGATGAA TAAATCCTAC CTGCTGGAGC CTTGGTTCCT GCCCCCCAGC 
CGAGGTCTCC AGAATTCACC AGCAGTTTCC CTGCCTGACC CAGAGTTCAA GAGGGACAGG 
AAAGGTCCCC ATCCTACTGC CAGGAGCCCT GGAACAGTCA TGGAAAGCAA TCCCCAACAG 
AGAGAGGGCA GCTCCCCTAA ACACCAAGAC CACACCACTG ACCCCAAGCT TCTGACCTGC 
CTGGGGCAGA ACCTCCACAG CCCTGACCTG GCCAGGCCAC GCTGCCCGCT CCCACCTGAA 
GCTTCCCCCT CAAGGGAGAA GCCAGGCCTG AGGGAATCGT CTGAAAGAGG CCCTCCCACA 
GCCAGAAGCG AGCGCTCTGC TGCGAGGGCT GACACATGCA GAGAGCCCTC CATGGAACTG 
TGCTTTCCAG AAACTGCGAA AACCAGTGAC AACTCCAAAA ATCTCCTCTC TGTGGGAAGG 
ACCCACCCAG ATTTCTATAC ACAGACCCAG GCCATGGAGA AAGCATGGGC GCCGGGTGGG 
AAAACGAACC ACAAAGATGG CCCAGGTGAG GCGAGGCCCC CGCCCAGAGA CAACTCCTCT 
CTGCACTCAG CTGGAATTCC CTGTGAGAAG GAGCTGGGCA AGGTGAGGCG TGGCGTGGAA 
CCCAAGCCCG AAGCGCTTCT TGCCAGGCGG TCTCTGCAGC CACCTGGAAT TGAGAGTGAG 
AAGAGTGAAA AGCTCTCCAG TTTCCCATCT TTGCAGAAAG ATGGTGCCAA GGAACCTGAA 
AGGAAGGAGC AGCCTCTACA AAGGCATCCC AGCAGCATCC CTCCGCCCCC TCTGACGGCC 
AAAGACCTGT CCAGCCCGGC TGCCAGGCAG CATTGCAGTT CCCCAAGCCA CGCTTCTGGC 
AGAGAGCCGG GGGCCAAGCC CAGCACTGCA GAGCCCAGCT CGAGCCCCCA GGACCCTCCC 
AAGCCTGTTG CTGCGCACAG TGAAAGCAGC AGCCACAAGC CCCGGCCTGG CCCTGACCCG 
GGCCCTCCAA AGACTAAGCA CCCCGACCGG TCCCTCTCCT CTCAGAAACC AAGTGTCGGG 
GCCACAAAGG GCAAAGAGCC TGCCACTCAG TCCCTCGGTG GCTCTAGCAG AGAGGGGAAG 
GGCCACAGTA AGAGTGGGCC GGATGTGTTT CCTGCTACCC CAGGCTCCCA GAACAAAGCC 
AGCGATGGGA TTGGCCAGGG AGAAGGTGGG CCCTCTGTCC CACTGCACAC TGACAGGGCT 
CCTCTAGACG CCAAGCCACA ACCCACCAGT GGTGGGCGGC CCCTGGAGGT GCTGGAGAAG 
CCTGTGCATT TGCCAAGGCC GGGACACCCA GGGCCTAGTG AGCCAGCGGA CCAGAAACTG 
TCCGCTGTTG GTGAAAAGCA AACCCTGTCT CCAAAGCACC CCAAACCATC CACTGTGAAA 
GATTGCCCCA CCCTGTGCAA ACAGACAGAC AACAGACAGA CAGACAAAAG CCCGAGTCAG 
CCGGCCGCCA ACACCGACAG AAGGGCGGAA GGGAAGAAAT GCACTGAAGC ACTTTATGCT 
CCAGCAGAGG GCGACAAGCT CGAGGCCGGC CTTTCCTTTG TGCATAGCGA GAACCGGTTG 
AAAGGCGCGG AGCGGCCAGC CGCGGGGGTG GGGAAGGGCT TCCCTGAGGC CAGAGGGAAA 
GGGCCCGGTC CCCAGAAGCC ACCGACGGAG GCAGACAAGC CCAATGGCAT GAAACGGTCC 
CCCTCAGCCA CTGGGCAGAG TTCTTTCCGA TCCACGGCCC TCCCGGAAAA GTCTCTGAGC 
TGCTCCTCCA GCTTCCCTGA AACCAGGGCC GGAGTTAGAG AGGCCTCTGC AGCCAGCAGC 
GACACCTCTT CTGCCAAGGC CGCCGGGGGC ATGCTGGAGC TTCCAGCCCC CAGCAACAGG 
GACCATAGGA AGGCTCAGCC TGCCGGGGAG GGCCGAACCC ACATGACAAA GAGTGACTCC 
CTGCCCTCCT TCCGGGTCTC CACCCTGCCT CTGGAGTCAC ACCACCCCGA CCCAAACACC 
ATGGGCGGGG CCAGCCACCG GGACAGGGCT CTCTCGGTGA CTGCCACCGT AGGGGAAACC 
AAAGGGAAGG ACCCTGCCCC AGCCCAGCCT CCCCCAGCTA GGAAACAGAA CGTGGGCAGA 
GACGTGACCA AGCCATCCCC AGCCCCAAAC ACTGACCGCC CCATCTCTCT TTCTAATGAG 
AAGGACTTTG TGGTACGGCA GAGGCGGGGG AAAGAGAGTT TGCGTAGCAG CCCTCACAAA 
AAGGCCTTGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_024446044.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024301812.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.

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CloneID	OHu105665
Clone ID Related Accession (Same CDS sequence)	XM_024446044.1
Accession Version	XM_024446044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGTA TGGATGAACG ATCCAATTGC 
TGGAGGTACC TTCTTCCAAA CCCGGTGGCG GGACAGGCCT GGCCGGCCTC TGCAGAGACG 
TCCAACCTCG TGCGCATGCG CAGCCAGGCC CTGGGCCAGT CGGCGCCCTC GCTCACCGCC 
AGCCTGGAAG GGCAGCCTCC GAAGAGCCAT TTCAACTCAG CCCGACATCG CCAGAGACTA 
GTGGACCCAG CTGCTTCAAA AAAGGAGCTG AGTCTCCCCA GAAGAGGAAG TTTGATAGAT 
TCCCAGAAGT GGAATTGCTT GGTCAAACGT CTCACTCCCA GGAGCCTGAG TCCAACCCCA 
TCCAGCCCAG GCAGTCCTTG TAGTCCTCTC TTGGCCTTTC ACTTTTGGAG TTGCCGAACA 
AGCAACCGGA AAAGCTTAAT AGGCAATGGG CAGTCACCAG CATTGCCTCG ACCACACTCA 
CCTCTCTCTG CTCATGCAGG AAATAGCCCT CAAGATAGTC CAAGAAATTT CTCCCCCAGT 
GCCTCAGCCC ATTTTTCATT TGCACGGAGG ACTGATGGAC GCCGCTGGTC GTTGGCTTCT 
CTCCCTTCCT CTGGCTATGG GACAAACACA CCCAGCTCTA CGGTCTCTTC ATCCTGTTCC 
TCCCAGGAGA AGTTGCATCA GTTACCATAC CAACCAACAC CAGACGAGTT ACACTTCTTA 
TCAAAACATT TCTGTACCAC CGAAAGCATC GCCACTGAGA ACAGATGCAG GAACACGCCG 
ATGCGCCCCC GTTCCCGAAG TCTGAGCCCT GGACGTTCTC CCGCCTGCTG TGACCATGAA 
ATAATTATGA TGAACCATGT CTACAAAGAA AGGTTCCCAA AGGCTACAGC TCAGATGGAA 
GAACGTCTAA AGGAAATTAT CACCAGCTAC TCTCCTGACA ACGTTCTACC CTTAGCAGAT 
GGAGTGCTTA GTTTCACTCA CCACCAGATT ATTGAACTGG CTCGAGATTG CTTGGATAAA 
TCCCACCAGG GCCTCATCAC CTCACGATAC TTCCTTGAAT TACAGCACAA ATTAGATAAG 
TTGCTACAGG AGGCTCATGA TCGTTCAGAA AGTGGAGAAT TGGCATTTAT TAAACAACTA 
GTTCGAAAGA TCCTAATTGT TATTGCCCGC CCTGCTCGGT TATTAGAGTG CCTGGAATTT 
GATCCGGAAG AATTTTACTA CCTATTGGAA GCAGCAGAAG GCCATGCCAA AGAAGGACAG 
GGTATTAAAA CCGACATTCC CAGGTACATC ATTAGCCAAC TGGGACTCAA TAAGGATCCC 
TTGGAAGAAA TGGCTCATTT GGGAAACTAC GATAGTGGGA CAGCAGAAAC ACCAGAAACA 
GATGAATCAG TGAGTAGCTC TAATGCCTCC CTGAAACTTC GAAGGAAACC TCGGGAAAGT 
GATTTTGAAA CGATTAAATT GATTAGCAAT GGAGCCTATG GGGCAGTCTA CTTTGTTCGG 
CATAAAGAAT CCCGGCAGAG GTTTGCCATG AAGAAGATTA ATAAACAGAA CCTCATCCTT 
CGAAACCAGA TCCAGCAGGC CTTTGTGGAG CGGGATATCC TGACTTTTGC AGAAAACCCC 
TTTGTTGTCA GCATGTATTG CTCCTTTGAA ACAAGGCGCC ACTTGTGCAT GGTCATGGAA 
TATGTGGAAG GGGGAGACTG TGCTACTTTA ATGAAAAACA TGGGTCCTCT CCCTGTTGAT 
ATGGCCAGAA TGTACTTTGC TGAGACGGTC TTGGCCTTGG AATATTTACA TAATTATGGA 
ATTGTACACA GGGATTTGAA ACCAGACAAC TTGTTGGTTA CCTCCATGGG GCACATAAAG 
CTGACAGATT TTGGATTATC TAAGGTGGGA CTAATGAGCA TGACTACCAA CCTTTACGAG 
GGTCATATTG AGAAGGATGC TAGAGAGTTC CTGGATAAAC AGGTCTGTGG CACACCTGAA 
TACATTGCAC CAGAAGTGAT TCTGAGGCAG GGTTATGGAA AGCCGGTGGA CTGGTGGGCC 
ATGGGGATTA TCCTCTATGA ATTTCTGGTT GGATGCGTGC CATTCTTTGG GGATACTCCA 
GAGGAGCTAT TTGGACAAGT CATCAGTGAT GAGATCAACT GGCCTGAGAA GGATGAGGCA 
CCCCCACCTG ATGCCCAGGA TCTGATTACC TTACTCCTCA GGCAGAATCC CCTGGAGAGG 
CTGGGAACAG GTGGTGCATA TGAAGTCAAA CAGCATCGAT TCTTCCGTTC TTTAGACTGG 
AACAGTTTGC TGAGACAGAA GGCAGAATTT ATTCCCCAAC TGGAATCTGA GGATGACACA 
AGTTATTTTG ATACTCGGTC TGAGAAGTAT CATCATATGG AAACGGAGGA AGAAGATGAC 
ACAAATGATG AAGACTTTAA TGTGGAAATA AGGCAGTTTT CTTCATGTTC ACACAGGTTT 
TCAAAAGTTT TCAGCAGTAT AGATCGAATC ACTCAGAATT CAGCAGAAGA GAAGGAAGAC 
TCTGTGGACA AAACCAAAAG CACCACCTTG CCATCCACAG AAACACTGAG CTGGAGTTCA 
GAATATTCTG AAATGCAACA GCTATCAACA TCCAACTCTT CAGATACTGA AAGCAACAGA 
CATAAACTCA GTTCTGGCCT ACTTCCCAAA CTGGCTATTT CAACAGAGGG AGAGCAAGAT 
GAAGCTGCCT CCTGCCCTGG AGACCCCCAT GAGGAGCCAG GAAAGCCAGC CCTTCCTCCT 
GAAGAGTGTG CCCAGGAGGA GCCTGAGGTC ACCACCCCAG CCAGCACCAT CAGCAGCTCC 
ACCCTGTCAG TTGGCAGTTT TTCAGAGCAC TTGGATCAGA TAAATGGACG AAGCGAGTGT 
GTGGACAGTA CAGATAATTC CTCAAAGCCA TCCAGTGAAC CCGCTTCTCA CATGGCTCGG 
CAGCGATTAG AAAGCACAGA AAAAAAGAAA ATCTCGGGGA AAGTCACAAA GTCCCTCTCT 
GCCAGTGCTC TTTCCCTCAT GATCCCAGGA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024301812.1
CDS	303..8390
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGMDERSNCWRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLIDSQKWNCLVKRLTPRSLSPTPSSPGSPCSPLLAFHFWSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_024446044.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446044.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGTA TGGATGAACG ATCCAATTGC 
TGGAGGTACC TTCTTCCAAA CCCGGTGGCG GGACAGGCCT GGCCGGCCTC TGCAGAGACG 
TCCAACCTCG TGCGCATGCG CAGCCAGGCC CTGGGCCAGT CGGCGCCCTC GCTCACCGCC 
AGCCTGGAAG GGCAGCCTCC GAAGAGCCAT TTCAACTCAG CCCGACATCG CCAGAGACTA 
GTGGACCCAG CTGCTTCAAA AAAGGAGCTG AGTCTCCCCA GAAGAGGAAG TTTGATAGAT 
TCCCAGAAGT GGAATTGCTT GGTCAAACGT CTCACTCCCA GGAGCCTGAG TCCAACCCCA 
TCCAGCCCAG GCAGTCCTTG TAGTCCTCTC TTGGCCTTTC ACTTTTGGAG TTGCCGAACA 
AGCAACCGGA AAAGCTTAAT AGGCAATGGG CAGTCACCAG CATTGCCTCG ACCACACTCA 
CCTCTCTCTG CTCATGCAGG AAATAGCCCT CAAGATAGTC CAAGAAATTT CTCCCCCAGT 
GCCTCAGCCC ATTTTTCATT TGCACGGAGG ACTGATGGAC GCCGCTGGTC GTTGGCTTCT 
CTCCCTTCCT CTGGCTATGG GACAAACACA CCCAGCTCTA CGGTCTCTTC ATCCTGTTCC 
TCCCAGGAGA AGTTGCATCA GTTACCATAC CAACCAACAC CAGACGAGTT ACACTTCTTA 
TCAAAACATT TCTGTACCAC CGAAAGCATC GCCACTGAGA ACAGATGCAG GAACACGCCG 
ATGCGCCCCC GTTCCCGAAG TCTGAGCCCT GGACGTTCTC CCGCCTGCTG TGACCATGAA 
ATAATTATGA TGAACCATGT CTACAAAGAA AGGTTCCCAA AGGCTACAGC TCAGATGGAA 
GAACGTCTAA AGGAAATTAT CACCAGCTAC TCTCCTGACA ACGTTCTACC CTTAGCAGAT 
GGAGTGCTTA GTTTCACTCA CCACCAGATT ATTGAACTGG CTCGAGATTG CTTGGATAAA 
TCCCACCAGG GCCTCATCAC CTCACGATAC TTCCTTGAAT TACAGCACAA ATTAGATAAG 
TTGCTACAGG AGGCTCATGA TCGTTCAGAA AGTGGAGAAT TGGCATTTAT TAAACAACTA 
GTTCGAAAGA TCCTAATTGT TATTGCCCGC CCTGCTCGGT TATTAGAGTG CCTGGAATTT 
GATCCGGAAG AATTTTACTA CCTATTGGAA GCAGCAGAAG GCCATGCCAA AGAAGGACAG 
GGTATTAAAA CCGACATTCC CAGGTACATC ATTAGCCAAC TGGGACTCAA TAAGGATCCC 
TTGGAAGAAA TGGCTCATTT GGGAAACTAC GATAGTGGGA CAGCAGAAAC ACCAGAAACA 
GATGAATCAG TGAGTAGCTC TAATGCCTCC CTGAAACTTC GAAGGAAACC TCGGGAAAGT 
GATTTTGAAA CGATTAAATT GATTAGCAAT GGAGCCTATG GGGCAGTCTA CTTTGTTCGG 
CATAAAGAAT CCCGGCAGAG GTTTGCCATG AAGAAGATTA ATAAACAGAA CCTCATCCTT 
CGAAACCAGA TCCAGCAGGC CTTTGTGGAG CGGGATATCC TGACTTTTGC AGAAAACCCC 
TTTGTTGTCA GCATGTATTG CTCCTTTGAA ACAAGGCGCC ACTTGTGCAT GGTCATGGAA 
TATGTGGAAG GGGGAGACTG TGCTACTTTA ATGAAAAACA TGGGTCCTCT CCCTGTTGAT 
ATGGCCAGAA TGTACTTTGC TGAGACGGTC TTGGCCTTGG AATATTTACA TAATTATGGA 
ATTGTACACA GGGATTTGAA ACCAGACAAC TTGTTGGTTA CCTCCATGGG GCACATAAAG 
CTGACAGATT TTGGATTATC TAAGGTGGGA CTAATGAGCA TGACTACCAA CCTTTACGAG 
GGTCATATTG AGAAGGATGC TAGAGAGTTC CTGGATAAAC AGGTCTGTGG CACACCTGAA 
TACATTGCAC CAGAAGTGAT TCTGAGGCAG GGTTATGGAA AGCCGGTGGA CTGGTGGGCC 
ATGGGGATTA TCCTCTATGA ATTTCTGGTT GGATGCGTGC CATTCTTTGG GGATACTCCA 
GAGGAGCTAT TTGGACAAGT CATCAGTGAT GAGATCAACT GGCCTGAGAA GGATGAGGCA 
CCCCCACCTG ATGCCCAGGA TCTGATTACC TTACTCCTCA GGCAGAATCC CCTGGAGAGG 
CTGGGAACAG GTGGTGCATA TGAAGTCAAA CAGCATCGAT TCTTCCGTTC TTTAGACTGG 
AACAGTTTGC TGAGACAGAA GGCAGAATTT ATTCCCCAAC TGGAATCTGA GGATGACACA 
AGTTATTTTG ATACTCGGTC TGAGAAGTAT CATCATATGG AAACGGAGGA AGAAGATGAC 
ACAAATGATG AAGACTTTAA TGTGGAAATA AGGCAGTTTT CTTCATGTTC ACACAGGTTT 
TCAAAAGTTT TCAGCAGTAT AGATCGAATC ACTCAGAATT CAGCAGAAGA GAAGGAAGAC 
TCTGTGGACA AAACCAAAAG CACCACCTTG CCATCCACAG AAACACTGAG CTGGAGTTCA 
GAATATTCTG AAATGCAACA GCTATCAACA TCCAACTCTT CAGATACTGA AAGCAACAGA 
CATAAACTCA GTTCTGGCCT ACTTCCCAAA CTGGCTATTT CAACAGAGGG AGAGCAAGAT 
GAAGCTGCCT CCTGCCCTGG AGACCCCCAT GAGGAGCCAG GAAAGCCAGC CCTTCCTCCT 
GAAGAGTGTG CCCAGGAGGA GCCTGAGGTC ACCACCCCAG CCAGCACCAT CAGCAGCTCC 
ACCCTGTCAG TTGGCAGTTT TTCAGAGCAC TTGGATCAGA TAAATGGACG AAGCGAGTGT 
GTGGACAGTA CAGATAATTC CTCAAAGCCA TCCAGTGAAC CCGCTTCTCA CATGGCTCGG 
CAGCGATTAG AAAGCACAGA AAAAAAGAAA ATCTCGGGGA AAGTCACAAA GTCCCTCTCT 
GCCAGTGCTC TTTCCCTCAT GATCCCAGGA GATATGTTTG CTGTTTCCCC TCTGGGAAGT 
CCAATGTCTC CCCATTCCCT GTCCTCGGAC CCTTCTTCTT CACGAGATTC CTCTCCCAGC 
CGAGATTCCT CAGCAGCTTC TGCCAGTCCA CATCAGCCGA TTGTGATCCA CAGTTCGGGG 
AAGAACTACG GCTTTACCAT CCGAGCCATC CGGGTGTATG TGGGAGACAG TGACATCTAT 
ACAGTGCACC ATATCGTCTG GAATGTAGAA GAAGGAAGTC CGGCATGCCA GGCAGGACTG 
AAGGCTGGAG ATCTTATCAC TCACATCAAT GGAGAACCAG TGCATGGACT TGTCCACACA 
GAAGTTATAG AACTCCTACT GAAGAGTGGG AATAAGGTGT CAATCACTAC TACCCCATTT 
GAAAACACAT CAATCAAAAC TGGACCAGCC AGGAGAAACA GCTATAAGAG CCGGATGGTG 
AGGCGGAGCA AGAAATCCAA GAAGAAAGAA AGTCTCGAAA GGAGGAGATC TCTTTTCAAA 
AAGCTAGCCA AGCAGCCTTC TCCTTTACTC CACACCAGCC GAAGTTTCTC CTGCTTGAAC 
AGATCCCTGT CATCGGGTGA GAGCCTCCCA GGTTCCCCCA CTCATAGCTT GTCTCCCCGG 
TCTCCAACAC CAAGCTACCG CTCCACCCCT GACTTCCCAT CTGGTACTAA TTCCTCCCAG 
AGCAGCTCCC CTAGTTCTAG TGCCCCCAAT TCCCCAGCAG GGTCCGGGCA CATCCGGCCC 
AGCACTCTCC ACGGTCTTGC ACCCAAACTC GGCGGGCAGC GGTACCGGTC CGGAAGGCGA 
AAGTCCGCCG GCAACATCCC ACTGTCCCCG CTGGCCCGGA CGCCCTCTCC AACCCCGCAA 
CCCACCTCCC CGCAGCGGTC ACCATCCCCT CTTCTGGGAC ACTCACTGGG CAATTCCAAG 
ATCGCGCAAG CCTTTCCCAG CAAGATGCAC TCCCCGCCCA CCATCGTCAG ACACATCGTG 
AGGCCCAAGA GTGCGGAGCC CCCCAGGTCC CCGCTGCTCA AGCGCGTGCA GTCCGAGGAG 
AAGCTGTCGC CCTCTTACGG CAGTGACAAG AAGCACCTGT GCTCCCGCAA GCACAGCCTG 
GAGGTGACCC AAGAGGAGGT GCAGCGGGAG CAGTCCCAGC GGGAGGCGCC GCTGCAGAGC 
CTGGATGAGA ACGTGTGCGA CGTGCCGCCG CTCAGCCGCG CCCGGCCAGT GGAGCAAGGC 
TGCCTGAAAC GCCCAGTCTC CCGGAAGGTG GGCCGCCAGG AGTCTGTGGA CGACCTGGAC 
CGCGACAAGC TGAAGGCCAA GGTGGTGGTG AAGAAAGCAG ACGGCTTCCC AGAGAAACAG 
GAATCCCACC AGAAATCCCA TGGACCCGGG AGTGATTTGG AAAACTTTGC TCTGTTTAAG 
CTGGAAGAGA GAGAGAAGAA AGTCTATCCG AAGGCTGTGG AAAGGTCAAG TACTTTTGAA 
AACAAAGCGT CTATGCAGGA GGCGCCACCG CTGGGCAGCC TGCTGAAGGA TGCTCTTCAC 
AAGCAGGCCA GCGTGCGCGC CAGCGAGGGT GCGATGTCGG ATGGCCGGGT GCCTGCGGAG 
CACCGCCAGG GTGGCGGGGA CTTCAGACGG GCCCCCGCTC CTGGCACCCT CCAGGATGGT 
CTCTGCCACT CCCTCGACAG GGGCATCTCT GGGAAGGGGG AAGGCACGGA GAAGTCCTCC 
CAGGCCAAGG AGCTTCTCCG ATGTGAAAAG TTAGACAGCA AGCTGGCCAA CATCGATTAC 
CTCCGAAAGA AAATGTCACT TGAGGACAAA GAGGACAACC TCTGCCCTGT GCTGAAGCCC 
AAGATGACAG CTGGCTCCCA CGAATGCCTG CCAGGGAACC CAGTCCGACC CACGGGTGGG 
CAGCAGGAGC CCCCGCCGGC TTCTGAGAGC CGAGCTTTTG TCAGCAGCAC CCATGCAGCT 
CAGATGAGTG CCGTCTCTTT TGTTCCCCTC AAGGCCTTAA CAGGCCGGGT GGACAGTGGA 
ACGGAGAAGC CTGGCTTGGT TGCTCCTGAG TCCCCTGTTA GGAAGAGCCC CTCCGAGTAT 
AAGCTGGAAG GTAGGTCTGT CTCATGCCTG AAGCCGATCG AGGGCACTCT GGACATTGCT 
CTCCTGTCCG GACCTCAGGC CTCCAAGACA GAACTGCCTT CCCCAGAGTC TGCACAGAGC 
CCCAGCCCAA GTGGTGACGT GAGGGCCTCT GTGCCACCAG TTCTCCCCAG CAGCAGTGGG 
AAAAAGAACG ATACCACCAG TGCAAGAGAG CTTTCTCCTT CCAGCTTAAA GATGAATAAA 
TCCTACCTGC TGGAGCCTTG GTTCCTGCCC CCCAGCCGAG GTCTCCAGAA TTCACCAGCA 
GTTTCCCTGC CTGACCCAGA GTTCAAGAGG GACAGGAAAG GTCCCCATCC TACTGCCAGG 
AGCCCTGGAA CAGTCATGGA AAGCAATCCC CAACAGAGAG AGGGCAGCTC CCCTAAACAC 
CAAGACCACA CCACTGACCC CAAGCTTCTG ACCTGCCTGG GGCAGAACCT CCACAGCCCT 
GACCTGGCCA GGCCACGCTG CCCGCTCCCA CCTGAAGCTT CCCCCTCAAG GGAGAAGCCA 
GGCCTGAGGG AATCGTCTGA AAGAGGCCCT CCCACAGCCA GAAGCGAGCG CTCTGCTGCG 
AGGGCTGACA CATGCAGAGA GCCCTCCATG GAACTGTGCT TTCCAGAAAC TGCGAAAACC 
AGTGACAACT CCAAAAATCT CCTCTCTGTG GGAAGGACCC ACCCAGATTT CTATACACAG 
ACCCAGGCCA TGGAGAAAGC ATGGGCGCCG GGTGGGAAAA CGAACCACAA AGATGGCCCA 
GGTGAGGCGA GGCCCCCGCC CAGAGACAAC TCCTCTCTGC ACTCAGCTGG AATTCCCTGT 
GAGAAGGAGC TGGGCAAGGT GAGGCGTGGC GTGGAACCCA AGCCCGAAGC GCTTCTTGCC 
AGGCGGTCTC TGCAGCCACC TGGAATTGAG AGTGAGAAGA GTGAAAAGCT CTCCAGTTTC 
CCATCTTTGC AGAAAGATGG TGCCAAGGAA CCTGAAAGGA AGGAGCAGCC TCTACAAAGG 
CATCCCAGCA GCATCCCTCC GCCCCCTCTG ACGGCCAAAG ACCTGTCCAG CCCGGCTGCC 
AGGCAGCATT GCAGTTCCCC AAGCCACGCT TCTGGCAGAG AGCCGGGGGC CAAGCCCAGC 
ACTGCAGAGC CCAGCTCGAG CCCCCAGGAC CCTCCCAAGC CTGTTGCTGC GCACAGTGAA 
AGCAGCAGCC ACAAGCCCCG GCCTGGCCCT GACCCGGGCC CTCCAAAGAC TAAGCACCCC 
GACCGGTCCC TCTCCTCTCA GAAACCAAGT GTCGGGGCCA CAAAGGGCAA AGAGCCTGCC 
ACTCAGTCCC TCGGTGGCTC TAGCAGAGAG GGGAAGGGCC ACAGTAAGAG TGGGCCGGAT 
GTGTTTCCTG CTACCCCAGG CTCCCAGAAC AAAGCCAGCG ATGGGATTGG CCAGGGAGAA 
GGTGGGCCCT CTGTCCCACT GCACACTGAC AGGGCTCCTC TAGACGCCAA GCCACAACCC 
ACCAGTGGTG GGCGGCCCCT GGAGGTGCTG GAGAAGCCTG TGCATTTGCC AAGGCCGGGA 
CACCCAGGGC CTAGTGAGCC AGCGGACCAG AAACTGTCCG CTGTTGGTGA AAAGCAAACC 
CTGTCTCCAA AGCACCCCAA ACCATCCACT GTGAAAGATT GCCCCACCCT GTGCAAACAG 
ACAGACAACA GACAGACAGA CAAAAGCCCG AGTCAGCCGG CCGCCAACAC CGACAGAAGG 
GCGGAAGGGA AGAAATGCAC TGAAGCACTT TATGCTCCAG CAGAGGGCGA CAAGCTCGAG 
GCCGGCCTTT CCTTTGTGCA TAGCGAGAAC CGGTTGAAAG GCGCGGAGCG GCCAGCCGCG 
GGGGTGGGGA AGGGCTTCCC TGAGGCCAGA GGGAAAGGGC CCGGTCCCCA GAAGCCACCG 
ACGGAGGCAG ACAAGCCCAA TGGCATGAAA CGGTCCCCCT CAGCCACTGG GCAGAGTTCT 
TTCCGATCCA CGGCCCTCCC GGAAAAGTCT CTGAGCTGCT CCTCCAGCTT CCCTGAAACC 
AGGGCCGGAG TTAGAGAGGC CTCTGCAGCC AGCAGCGACA CCTCTTCTGC CAAGGCCGCC 
GGGGGCATGC TGGAGCTTCC AGCCCCCAGC AACAGGGACC ATAGGAAGGC TCAGCCTGCC 
GGGGAGGGCC GAACCCACAT GACAAAGAGT GACTCCCTGC CCTCCTTCCG GGTCTCCACC 
CTGCCTCTGG AGTCACACCA CCCCGACCCA AACACCATGG GCGGGGCCAG CCACCGGGAC 
AGGGCTCTCT CGGTGACTGC CACCGTAGGG GAAACCAAAG GGAAGGACCC TGCCCCAGCC 
CAGCCTCCCC CAGCTAGGAA ACAGAACGTG GGCAGAGACG TGACCAAGCC ATCCCCAGCC 
CCAAACACTG ACCGCCCCAT CTCTCTTTCT AATGAGAAGG ACTTTGTGGT ACGGCAGAGG 
CGGGGGAAAG AGAGTTTGCG TAGCAGCCCT CACAAAAAGG CCTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_011543382.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541684.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.

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CloneID	OHu79753
Clone ID Related Accession (Same CDS sequence)	XM_011543382.2
Accession Version	XM_011543382.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543382.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGGAAGGGCA GCCTCCGAAG 
AGCCATTTCA ACTCAGCCCG ACATCGCCAG AGACTAGTGG ACCCAGCTGC TTCAAAAAAG 
GAGCTGAGTC TCCCCAGAAG AGGAAGTTTG ATAGATTCCC AGAAGTGGAA TTGCTTGGTC 
AAACGTTGCC GAACAAGCAA CCGGAAAAGC TTAATAGGCA ATGGGCAGTC ACCAGCATTG 
CCTCGACCAC ACTCACCTCT CTCTGCTCAT GCAGGAAATA GCCCTCAAGA TAGTCCAAGA 
AATTTCTCCC CCAGTGCCTC AGCCCATTTT TCATTTGCAC GGAGGACTGA TGGACGCCGC 
TGGTCGTTGG CTTCTCTCCC TTCCTCTGGC TATGGGACAA ACACACCCAG CTCTACGGTC 
TCTTCATCCT GTTCCTCCCA GGAGAAGTTG CATCAGTTAC CATACCAACC AACACCAGAC 
GAGTTACACT TCTTATCAAA ACATTTCTGT ACCACCGAAA GCATCGCCAC TGAGAACAGA 
TGCAGGAACA CGCCGATGCG CCCCCGTTCC CGAAGTCTGA GCCCTGGACG TTCTCCCGCC 
TGCTGTGACC ATGAAATAAT TATGATGAAC CATGTCTACA AAGAAAGGTT CCCAAAGGCT 
ACAGCTCAGA TGGAAGAACG TCTAAAGGAA ATTATCACCA GCTACTCTCC TGACAACGTT 
CTACCCTTAG CAGATGGAGT GCTTAGTTTC ACTCACCACC AGATTATTGA ACTGGCTCGA 
GATTGCTTGG ATAAATCCCA CCAGGGCCTC ATCACCTCAC GATACTTCCT TGAATTACAG 
CACAAATTAG ATAAGTTGCT ACAGGAGGCT CATGATCGTT CAGAAAGTGG AGAATTGGCA 
TTTATTAAAC AACTAGTTCG AAAGATCCTA ATTGTTATTG CCCGCCCTGC TCGGTTATTA 
GAGTGCCTGG AATTTGATCC GGAAGAATTT TACTACCTAT TGGAAGCAGC AGAAGGCCAT 
GCCAAAGAAG GACAGGGTAT TAAAACCGAC ATTCCCAGGT ACATCATTAG CCAACTGGGA 
CTCAATAAGG ATCCCTTGGA AGAAATGGCT CATTTGGGAA ACTACGATAG TGGGACAGCA 
GAAACACCAG AAACAGATGA ATCAGTGAGT AGCTCTAATG CCTCCCTGAA ACTTCGAAGG 
AAACCTCGGG AAAGTGATTT TGAAACGATT AAATTGATTA GCAATGGAGC CTATGGGGCA 
GTCTACTTTG TTCGGCATAA AGAATCCCGG CAGAGGTTTG CCATGAAGAA GATTAATAAA 
CAGAACCTCA TCCTTCGAAA CCAGATCCAG CAGGCCTTTG TGGAGCGGGA TATCCTGACT 
TTTGCAGAAA ACCCCTTTGT TGTCAGCATG TATTGCTCCT TTGAAACAAG GCGCCACTTG 
TGCATGGTCA TGGAATATGT GGAAGGGGGA GACTGTGCTA CTTTAATGAA AAACATGGGT 
CCTCTCCCTG TTGATATGGC CAGAATGTAC TTTGCTGAGA CGGTCTTGGC CTTGGAATAT 
TTACATAATT ATGGAATTGT ACACAGGGAT TTGAAACCAG ACAACTTGTT GGTTACCTCC 
ATGGGGCACA TAAAGCTGAC AGATTTTGGA TTATCTAAGG TGGGACTAAT GAGCATGACT 
ACCAACCTTT ACGAGGGTCA TATTGAGAAG GATGCTAGAG AGTTCCTGGA TAAACAGGTC 
TGTGGCACAC CTGAATACAT TGCACCAGAA GTGATTCTGA GGCAGGGTTA TGGAAAGCCG 
GTGGACTGGT GGGCCATGGG GATTATCCTC TATGAATTTC TGGTTGGATG CGTGCCATTC 
TTTGGGGATA CTCCAGAGGA GCTATTTGGA CAAGTCATCA GTGATGAGAT CAACTGGCCT 
GAGAAGGATG AGGCACCCCC ACCTGATGCC CAGGATCTGA TTACCTTACT CCTCAGGCAG 
AATCCCCTGG AGAGGCTGGG AACAGGTGGT GCATATGAAG TCAAACAGCA TCGATTCTTC 
CGTTCTTTAG ACTGGAACAG TTTGCTGAGA CAGAAGGCAG AATTTATTCC CCAACTGGAA 
TCTGAGGATG ACACAAGTTA TTTTGATACT CGGTCTGAGA AGTATCATCA TATGGAAACG 
GAGGAAGAAG ATGACACAAA TGATGAAGAC TTTAATGTGG AAATAAGGCA GTTTTCTTCA 
TGTTCACACA GGTTTTCAAA AGTTTTCAGC AGTATAGATC GAATCACTCA GAATTCAGCA 
GAAGAGAAGG AAGACTCTGT GGACAAAACC AAAAGCACCA CCTTGCCATC CACAGAAACA 
CTGAGCTGGA GTTCAGAATA TTCTGAAATG CAACAGCTAT CAACATCCAA CTCTTCAGAT 
ACTGAAAGCA ACAGACATAA ACTCAGTTCT GGCCTACTTC CCAAACTGGC TATTTCAACA 
GAGGGAGAGC AAGATGAAGC TGCCTCCTGC CCTGGAGACC CCCATGAGGA GCCAGGAAAG 
CCAGCCCTTC CTCCTGAAGA GTGTGCCCAG GAGGAGCCTG AGGTCACCAC CCCAGCCAGC 
ACCATCAGCA GCTCCACCCT GTCAGTTGGC AGTTTTTCAG AGCACTTGGA TCAGATAAAT 
GGACGAAGCG AGTGTGTGGA CAGTACAGAT AATTCCTCAA AGCCATCCAG TGAACCCGCT 
TCTCACATGG CTCGGCAGCG ATTAGAAAGC ACAGAAAAAA AGAAAATCTC GGGGAAAGTC 
ACAAAGTCCC TCTCTGCCAG TGCTCTTTCC CTCATGATCC CAGGAGATAT GTTTGCTGTT 
TCCCCTCTGG GAAGTCCAAT GTCTCCCCAT TCCCTGTCCT CGGACCCTTC TTCTTCACGA 
GATTCCTCTC CCAGCCGAGA TTCCTCAGCA GCTTCTGCCA GTCCACATCA GCCGATTGTG 
ATCCACAGTT CGGGGAAGAA CTACGGCTTT ACCATCCGAG CCATCCGGGT GTATGTGGGA 
GACAGTGACA TCTATACAGT GCACCATATC GTCTGGAATG TAGAAGAAGG AAGTCCGGCA 
TGCCAGGCAG GACTGAAGGC TGGAGATCTT ATCACTCACA TCAATGGAGA ACCAGTGCAT 
GGACTTGTCC ACACAGAAGT TATAGAACTC CTACTGAAGA GTGGGAATAA GGTGTCAATC 
ACTACTACCC CATTTGAAAA CACATCAATC AAAACTGGAC CAGCCAGGAG AAACAGCTAT 
AAGAGCCGGA TGGTGAGGCG GAGCAAGAAA TCCAAGAAGA AAGAAAGTCT CGAAAGGAGG 
AGATCTCTTT TCAAAAAGCT AGCCAAGCAG CCTTCTCCTT TACTCCACAC CAGCCGAAGT 
TTCTCCTGCT TGAACAGATC CCTGTCATCG GGTGAGAGCC TCCCAGGTTC CCCCACTCAT 
AGCTTGTCTC CCCGGTCTCC AACACCAAGC TACCGCTCCA CCCCTGACTT CCCATCTGGT 
ACTAATTCCT CCCAGAGCAG CTCCCCTAGT TCTAGTGCCC CCAATTCCCC AGCAGGGTCC 
GGGCACATCC GGCCCAGCAC TCTCCACGGT CTTGCACCCA AACTCGGCGG GCAGCGGTAC 
CGGTCCGGAA GGCGAAAGTC CGCCGGCAAC ATCCCACTGT CCCCGCTGGC CCGGACGCCC 
TCTCCAACCC CGCAACCCAC CTCCCCGCAG CGGTCACCAT CCCCTCTTCT GGGACACTCA 
CTGGGCAATT CCAAGATCGC GCAAGCCTTT CCCAGCAAGA TGCACTCCCC GCCCACCATC 
GTCAGACACA TCGTGAGGCC CAAGAGTGCG GAGCCCCCCA GGTCCCCGCT GCTCAAGCGC 
GTGCAGTCCG AGGAGAAGCT GTCGCCCTCT TACGGCAGTG ACAAGAAGCA CCTGTGCTCC 
CGCAAGCACA GCCTGGAGGT GACCCAAGAG GAGGTGCAGC GGGAGCAGTC CCAGCGGGAG 
GCGCCGCTGC AGAGCCTGGA TGAGAACGTG TGCGACGTGC CGCCGCTCAG CCGCGCCCGG 
CCAGTGGAGC AAGGCTGCCT GAAACGCCCA GTCTCCCGGA AGGTGGGCCG CCAGGAGTCT 
GTGGACGACC TGGACCGCGA CAAGCTGAAG GCCAAGGTGG TGGTGAAGAA AGCAGACGGC 
TTCCCAGAGA AACAGGAATC CCACCAGAAA TCCCATGGAC CCGGGAGTGA TTTGGAAAAC 
TTTGCTCTGT TTAAGCTGGA AGAGAGAGAG AAGAAAGTCT ATCCGAAGGC TGTGGAAAGG 
TCAAGTACTT TTGAAAACAA AGCGTCTATG CAGGAGGCGC CACCGCTGGG CAGCCTGCTG 
AAGGATGCTC TTCACAAGCA GGCCAGCGTG CGCGCCAGCG AGGGTGCGAT GTCGGATGGC 
CGGGTGCCTG CGGAGCACCG CCAGGGTGGC GGGGACTTCA GACGGGCCCC CGCTCCTGGC 
ACCCTCCAGG ATGGTCTCTG CCACTCCCTC GACAGGGGCA TCTCTGGGAA GGGGGAAGGC 
ACGGAGAAGT CCTCCCAGGC CAAGGAGCTT CTCCGATGTG AAAAGTTAGA CAGCAAGCTG 
GCCAACATCG ATTACCTCCG AAAGAAAATG TCACTTGAGG ACAAAGAGGA CAACCTCTGC 
CCTGTGCTGA AGCCCAAGAT GACAGCTGGC TCCCACGAAT GCCTGCCAGG GAACCCAGTC 
CGACCCACGG GTGGGCAGCA GGAGCCCCCG CCGGCTTCTG AGAGCCGAGC TTTTGTCAGC 
AGCACCCATG CAGCTCAGAT GAGTGCCGTC TCTTTTGTTC CCCTCAAGGC CTTAACAGGC 
CGGGTGGACA GTGGAACGGA GAAGCCTGGC TTGGTTGCTC CTGAGTCCCC TGTTAGGAAG 
AGCCCCTCCG AGTATAAGCT GGAAGGTAGG TCTGTCTCAT GCCTGAAGCC GATCGAGGGC 
ACTCTGGACA TTGCTCTCCT GTCCGGACCT CAGGCCTCCA AGACAGAACT GCCTTCCCCA 
GAGTCTGCAC AGAGCCCCAG CCCAAGTGGT GACGTGAGGG CCTCTGTGCC ACCAGTTCTC 
CCCAGCAGCA GTGGGAAAAA GAACGATACC ACCAGTGCAA GAGAGCTTTC TCCTTCCAGC 
TTAAAGATGA ATAAATCCTA CCTGCTGGAG CCTTGGTTCC TGCCCCCCAG CCGAGGTCTC 
CAGAATTCAC CAGCAGTTTC CCTGCCTGAC CCAGAGTTCA AGAGGGACAG GAAAGGTCCC 
CATCCTACTG CCAGGAGCCC TGGAACAGTC ATGGAAAGCA ATCCCCAACA GAGAGAGGGC 
AGCTCCCCTA AACACCAAGA CCACACCACT GACCCCAAGC TTCTGACCTG CCTGGGGCAG 
AACCTCCACA GCCCTGACCT GGCCAGGCCA CGCTGCCCGC TCCCACCTGA AGCTTCCCCC 
TCAAGGGAGA AGCCAGGCCT GAGGGAATCG TCTGAAAGAG GCCCTCCCAC AGCCAGAAGC 
GAGCGCTCTG CTGCGAGGGC TGACACATGC AGAGAGCCCT CCATGGAACT GTGCTTTCCA 
GAAACTGCGA AAACCAGTGA CAACTCCAAA AATCTCCTCT CTGTGGGAAG GACCCACCCA 
GATTTCTATA CACAGACCCA GGCCATGGAG AAAGCATGGG CGCCGGGTGG GAAAACGAAC 
CACAAAGATG GCCCAGGTGA GGCGAGGCCC CCGCCCAGAG ACAACTCCTC TCTGCACTCA 
GCTGGAATTC CCTGTGAGAA GGAGCTGGGC AAGGTGAGGC GTGGCGTGGA ACCCAAGCCC 
GAAGCGCTTC TTGCCAGGCG GTCTCTGCAG CCACCTGGAA TTGAGAGTGA GAAGAGTGAA 
AAGCTCTCCA GTTTCCCATC TTTGCAGAAA GATGGTGCCA AGGAACCTGA AAGGAAGGAG 
CAGCCTCTAC AAAGGCATCC CAGCAGCATC CCTCCGCCCC CTCTGACGGC CAAAGACCTG 
TCCAGCCCGG CTGCCAGGCA GCATTGCAGT TCCCCAAGCC ACGCTTCTGG CAGAGAGCCG 
GGGGCCAAGC CCAGCACTGC AGAGCCCAGC TCGAGCCCCC AGGACCCTCC CAAGCCTGTT 
GCTGCGCACA GTGAAAGCAG CAGCCACAAG CCCCGGCCTG GCCCTGACCC GGGCCCTCCA 
AAGACTAAGC ACCCCGACCG GTCCCTCTCC TCTCAGAAAC CAAGTGTCGG GGCCACAAAG 
GGCAAAGAGC CTGCCACTCA GTCCCTCGGT GGCTCTAGCA GAGAGGGGAA GGGCCACAGT 
AAGAGTGGGC CGGATGTGTT TCCTGCTACC CCAGGCTCCC AGAACAAAGC CAGCGATGGG 
ATTGGCCAGG GAGAAGGTGG GCCCTCTGTC CCACTGCACA CTGACAGGGC TCCTCTAGAC 
GCCAAGCCAC AACCCACCAG TGGTGGGCGG CCCCTGGAGG TGCTGGAGAA GCCTGTGCAT 
TTGCCAAGGC CGGGACACCC AGGGCCTAGT GAGCCAGCGG ACCAGAAACT GTCCGCTGTT 
GGTGAAAAGC AAACCCTGTC TCCAAAGCAC CCCAAACCAT CCACTGTGAA AGATTGCCCC 
ACCCTGTGCA AACAGACAGA CAACAGACAG ACAGACAAAA GCCCGAGTCA GCCGGCCGCC 
AACACCGACA GAAGGGCGGA AGGGAAGAAA TGCACTGAAG CACTTTATGC TCCAGCAGAG 
GGCGACAAGC TCGAGGCCGG CCTTTCCTTT GTGCATAGCG AGAACCGGTT GAAAGGCGCG 
GAGCGGCCAG CCGCGGGGGT GGGGAAGGGC TTCCCTGAGG CCAGAGGGAA AGGGCCCGGT 
CCCCAGAAGC CACCGACGGA GGCAGACAAG CCCAATGGCA TGAAACGGTC CCCCTCAGCC 
ACTGGGCAGA GTTCTTTCCG ATCCACGGCC CTCCCGGAAA AGTCTCTGAG CTGCTCCTCC 
AGCTTCCCTG AAACCAGGGC CGGAGTTAGA GAGGCCTCTG CAGCCAGCAG CGACACCTCT 
TCTGCCAAGG CCGCCGGGGG CATGCTGGAG CTTCCAGCCC CCAGCAACAG GGACCATAGG 
AAGGCTCAGC CTGCCGGGGA GGGCCGAACC CACATGACAA AGAGTGACTC CCTGCCCTCC 
TTCCGGGTCT CCACCCTGCC TCTGGAGTCA CACCACCCCG ACCCAAACAC CATGGGCGGG 
GCCAGCCACC GGGACAGGGC TCTCTCGGTG ACTGCCACCG TAGGGGAAAC CAAAGGGAAG 
GACCCTGCCC CAGCCCAGCC TCCCCCAGCT AGGAAACAGA ACGTGGGCAG AGACGTGACC 
AAGCCATCCC CAGCCCCAAA CACTGACCGC CCCATCTCTC TTTCTAATGA GAAGGACTTT 
GTGGTACGGC AGAGGCGGGG GAAAGAGAGT TTGCGTAGCA GCCCTCACAA AAAGGCCTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541684.1
CDS	290..8272
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLIDSQKWNCLVKRCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_011543382.2
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543382.2

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGGAAGGGCA GCCTCCGAAG 
AGCCATTTCA ACTCAGCCCG ACATCGCCAG AGACTAGTGG ACCCAGCTGC TTCAAAAAAG 
GAGCTGAGTC TCCCCAGAAG AGGAAGTTTG ATAGATTCCC AGAAGTGGAA TTGCTTGGTC 
AAACGTTGCC GAACAAGCAA CCGGAAAAGC TTAATAGGCA ATGGGCAGTC ACCAGCATTG 
CCTCGACCAC ACTCACCTCT CTCTGCTCAT GCAGGAAATA GCCCTCAAGA TAGTCCAAGA 
AATTTCTCCC CCAGTGCCTC AGCCCATTTT TCATTTGCAC GGAGGACTGA TGGACGCCGC 
TGGTCGTTGG CTTCTCTCCC TTCCTCTGGC TATGGGACAA ACACACCCAG CTCTACGGTC 
TCTTCATCCT GTTCCTCCCA GGAGAAGTTG CATCAGTTAC CATACCAACC AACACCAGAC 
GAGTTACACT TCTTATCAAA ACATTTCTGT ACCACCGAAA GCATCGCCAC TGAGAACAGA 
TGCAGGAACA CGCCGATGCG CCCCCGTTCC CGAAGTCTGA GCCCTGGACG TTCTCCCGCC 
TGCTGTGACC ATGAAATAAT TATGATGAAC CATGTCTACA AAGAAAGGTT CCCAAAGGCT 
ACAGCTCAGA TGGAAGAACG TCTAAAGGAA ATTATCACCA GCTACTCTCC TGACAACGTT 
CTACCCTTAG CAGATGGAGT GCTTAGTTTC ACTCACCACC AGATTATTGA ACTGGCTCGA 
GATTGCTTGG ATAAATCCCA CCAGGGCCTC ATCACCTCAC GATACTTCCT TGAATTACAG 
CACAAATTAG ATAAGTTGCT ACAGGAGGCT CATGATCGTT CAGAAAGTGG AGAATTGGCA 
TTTATTAAAC AACTAGTTCG AAAGATCCTA ATTGTTATTG CCCGCCCTGC TCGGTTATTA 
GAGTGCCTGG AATTTGATCC GGAAGAATTT TACTACCTAT TGGAAGCAGC AGAAGGCCAT 
GCCAAAGAAG GACAGGGTAT TAAAACCGAC ATTCCCAGGT ACATCATTAG CCAACTGGGA 
CTCAATAAGG ATCCCTTGGA AGAAATGGCT CATTTGGGAA ACTACGATAG TGGGACAGCA 
GAAACACCAG AAACAGATGA ATCAGTGAGT AGCTCTAATG CCTCCCTGAA ACTTCGAAGG 
AAACCTCGGG AAAGTGATTT TGAAACGATT AAATTGATTA GCAATGGAGC CTATGGGGCA 
GTCTACTTTG TTCGGCATAA AGAATCCCGG CAGAGGTTTG CCATGAAGAA GATTAATAAA 
CAGAACCTCA TCCTTCGAAA CCAGATCCAG CAGGCCTTTG TGGAGCGGGA TATCCTGACT 
TTTGCAGAAA ACCCCTTTGT TGTCAGCATG TATTGCTCCT TTGAAACAAG GCGCCACTTG 
TGCATGGTCA TGGAATATGT GGAAGGGGGA GACTGTGCTA CTTTAATGAA AAACATGGGT 
CCTCTCCCTG TTGATATGGC CAGAATGTAC TTTGCTGAGA CGGTCTTGGC CTTGGAATAT 
TTACATAATT ATGGAATTGT ACACAGGGAT TTGAAACCAG ACAACTTGTT GGTTACCTCC 
ATGGGGCACA TAAAGCTGAC AGATTTTGGA TTATCTAAGG TGGGACTAAT GAGCATGACT 
ACCAACCTTT ACGAGGGTCA TATTGAGAAG GATGCTAGAG AGTTCCTGGA TAAACAGGTC 
TGTGGCACAC CTGAATACAT TGCACCAGAA GTGATTCTGA GGCAGGGTTA TGGAAAGCCG 
GTGGACTGGT GGGCCATGGG GATTATCCTC TATGAATTTC TGGTTGGATG CGTGCCATTC 
TTTGGGGATA CTCCAGAGGA GCTATTTGGA CAAGTCATCA GTGATGAGAT CAACTGGCCT 
GAGAAGGATG AGGCACCCCC ACCTGATGCC CAGGATCTGA TTACCTTACT CCTCAGGCAG 
AATCCCCTGG AGAGGCTGGG AACAGGTGGT GCATATGAAG TCAAACAGCA TCGATTCTTC 
CGTTCTTTAG ACTGGAACAG TTTGCTGAGA CAGAAGGCAG AATTTATTCC CCAACTGGAA 
TCTGAGGATG ACACAAGTTA TTTTGATACT CGGTCTGAGA AGTATCATCA TATGGAAACG 
GAGGAAGAAG ATGACACAAA TGATGAAGAC TTTAATGTGG AAATAAGGCA GTTTTCTTCA 
TGTTCACACA GGTTTTCAAA AGTTTTCAGC AGTATAGATC GAATCACTCA GAATTCAGCA 
GAAGAGAAGG AAGACTCTGT GGACAAAACC AAAAGCACCA CCTTGCCATC CACAGAAACA 
CTGAGCTGGA GTTCAGAATA TTCTGAAATG CAACAGCTAT CAACATCCAA CTCTTCAGAT 
ACTGAAAGCA ACAGACATAA ACTCAGTTCT GGCCTACTTC CCAAACTGGC TATTTCAACA 
GAGGGAGAGC AAGATGAAGC TGCCTCCTGC CCTGGAGACC CCCATGAGGA GCCAGGAAAG 
CCAGCCCTTC CTCCTGAAGA GTGTGCCCAG GAGGAGCCTG AGGTCACCAC CCCAGCCAGC 
ACCATCAGCA GCTCCACCCT GTCAGTTGGC AGTTTTTCAG AGCACTTGGA TCAGATAAAT 
GGACGAAGCG AGTGTGTGGA CAGTACAGAT AATTCCTCAA AGCCATCCAG TGAACCCGCT 
TCTCACATGG CTCGGCAGCG ATTAGAAAGC ACAGAAAAAA AGAAAATCTC GGGGAAAGTC 
ACAAAGTCCC TCTCTGCCAG TGCTCTTTCC CTCATGATCC CAGGAGATAT GTTTGCTGTT 
TCCCCTCTGG GAAGTCCAAT GTCTCCCCAT TCCCTGTCCT CGGACCCTTC TTCTTCACGA 
GATTCCTCTC CCAGCCGAGA TTCCTCAGCA GCTTCTGCCA GTCCACATCA GCCGATTGTG 
ATCCACAGTT CGGGGAAGAA CTACGGCTTT ACCATCCGAG CCATCCGGGT GTATGTGGGA 
GACAGTGACA TCTATACAGT GCACCATATC GTCTGGAATG TAGAAGAAGG AAGTCCGGCA 
TGCCAGGCAG GACTGAAGGC TGGAGATCTT ATCACTCACA TCAATGGAGA ACCAGTGCAT 
GGACTTGTCC ACACAGAAGT TATAGAACTC CTACTGAAGA GTGGGAATAA GGTGTCAATC 
ACTACTACCC CATTTGAAAA CACATCAATC AAAACTGGAC CAGCCAGGAG AAACAGCTAT 
AAGAGCCGGA TGGTGAGGCG GAGCAAGAAA TCCAAGAAGA AAGAAAGTCT CGAAAGGAGG 
AGATCTCTTT TCAAAAAGCT AGCCAAGCAG CCTTCTCCTT TACTCCACAC CAGCCGAAGT 
TTCTCCTGCT TGAACAGATC CCTGTCATCG GGTGAGAGCC TCCCAGGTTC CCCCACTCAT 
AGCTTGTCTC CCCGGTCTCC AACACCAAGC TACCGCTCCA CCCCTGACTT CCCATCTGGT 
ACTAATTCCT CCCAGAGCAG CTCCCCTAGT TCTAGTGCCC CCAATTCCCC AGCAGGGTCC 
GGGCACATCC GGCCCAGCAC TCTCCACGGT CTTGCACCCA AACTCGGCGG GCAGCGGTAC 
CGGTCCGGAA GGCGAAAGTC CGCCGGCAAC ATCCCACTGT CCCCGCTGGC CCGGACGCCC 
TCTCCAACCC CGCAACCCAC CTCCCCGCAG CGGTCACCAT CCCCTCTTCT GGGACACTCA 
CTGGGCAATT CCAAGATCGC GCAAGCCTTT CCCAGCAAGA TGCACTCCCC GCCCACCATC 
GTCAGACACA TCGTGAGGCC CAAGAGTGCG GAGCCCCCCA GGTCCCCGCT GCTCAAGCGC 
GTGCAGTCCG AGGAGAAGCT GTCGCCCTCT TACGGCAGTG ACAAGAAGCA CCTGTGCTCC 
CGCAAGCACA GCCTGGAGGT GACCCAAGAG GAGGTGCAGC GGGAGCAGTC CCAGCGGGAG 
GCGCCGCTGC AGAGCCTGGA TGAGAACGTG TGCGACGTGC CGCCGCTCAG CCGCGCCCGG 
CCAGTGGAGC AAGGCTGCCT GAAACGCCCA GTCTCCCGGA AGGTGGGCCG CCAGGAGTCT 
GTGGACGACC TGGACCGCGA CAAGCTGAAG GCCAAGGTGG TGGTGAAGAA AGCAGACGGC 
TTCCCAGAGA AACAGGAATC CCACCAGAAA TCCCATGGAC CCGGGAGTGA TTTGGAAAAC 
TTTGCTCTGT TTAAGCTGGA AGAGAGAGAG AAGAAAGTCT ATCCGAAGGC TGTGGAAAGG 
TCAAGTACTT TTGAAAACAA AGCGTCTATG CAGGAGGCGC CACCGCTGGG CAGCCTGCTG 
AAGGATGCTC TTCACAAGCA GGCCAGCGTG CGCGCCAGCG AGGGTGCGAT GTCGGATGGC 
CGGGTGCCTG CGGAGCACCG CCAGGGTGGC GGGGACTTCA GACGGGCCCC CGCTCCTGGC 
ACCCTCCAGG ATGGTCTCTG CCACTCCCTC GACAGGGGCA TCTCTGGGAA GGGGGAAGGC 
ACGGAGAAGT CCTCCCAGGC CAAGGAGCTT CTCCGATGTG AAAAGTTAGA CAGCAAGCTG 
GCCAACATCG ATTACCTCCG AAAGAAAATG TCACTTGAGG ACAAAGAGGA CAACCTCTGC 
CCTGTGCTGA AGCCCAAGAT GACAGCTGGC TCCCACGAAT GCCTGCCAGG GAACCCAGTC 
CGACCCACGG GTGGGCAGCA GGAGCCCCCG CCGGCTTCTG AGAGCCGAGC TTTTGTCAGC 
AGCACCCATG CAGCTCAGAT GAGTGCCGTC TCTTTTGTTC CCCTCAAGGC CTTAACAGGC 
CGGGTGGACA GTGGAACGGA GAAGCCTGGC TTGGTTGCTC CTGAGTCCCC TGTTAGGAAG 
AGCCCCTCCG AGTATAAGCT GGAAGGTAGG TCTGTCTCAT GCCTGAAGCC GATCGAGGGC 
ACTCTGGACA TTGCTCTCCT GTCCGGACCT CAGGCCTCCA AGACAGAACT GCCTTCCCCA 
GAGTCTGCAC AGAGCCCCAG CCCAAGTGGT GACGTGAGGG CCTCTGTGCC ACCAGTTCTC 
CCCAGCAGCA GTGGGAAAAA GAACGATACC ACCAGTGCAA GAGAGCTTTC TCCTTCCAGC 
TTAAAGATGA ATAAATCCTA CCTGCTGGAG CCTTGGTTCC TGCCCCCCAG CCGAGGTCTC 
CAGAATTCAC CAGCAGTTTC CCTGCCTGAC CCAGAGTTCA AGAGGGACAG GAAAGGTCCC 
CATCCTACTG CCAGGAGCCC TGGAACAGTC ATGGAAAGCA ATCCCCAACA GAGAGAGGGC 
AGCTCCCCTA AACACCAAGA CCACACCACT GACCCCAAGC TTCTGACCTG CCTGGGGCAG 
AACCTCCACA GCCCTGACCT GGCCAGGCCA CGCTGCCCGC TCCCACCTGA AGCTTCCCCC 
TCAAGGGAGA AGCCAGGCCT GAGGGAATCG TCTGAAAGAG GCCCTCCCAC AGCCAGAAGC 
GAGCGCTCTG CTGCGAGGGC TGACACATGC AGAGAGCCCT CCATGGAACT GTGCTTTCCA 
GAAACTGCGA AAACCAGTGA CAACTCCAAA AATCTCCTCT CTGTGGGAAG GACCCACCCA 
GATTTCTATA CACAGACCCA GGCCATGGAG AAAGCATGGG CGCCGGGTGG GAAAACGAAC 
CACAAAGATG GCCCAGGTGA GGCGAGGCCC CCGCCCAGAG ACAACTCCTC TCTGCACTCA 
GCTGGAATTC CCTGTGAGAA GGAGCTGGGC AAGGTGAGGC GTGGCGTGGA ACCCAAGCCC 
GAAGCGCTTC TTGCCAGGCG GTCTCTGCAG CCACCTGGAA TTGAGAGTGA GAAGAGTGAA 
AAGCTCTCCA GTTTCCCATC TTTGCAGAAA GATGGTGCCA AGGAACCTGA AAGGAAGGAG 
CAGCCTCTAC AAAGGCATCC CAGCAGCATC CCTCCGCCCC CTCTGACGGC CAAAGACCTG 
TCCAGCCCGG CTGCCAGGCA GCATTGCAGT TCCCCAAGCC ACGCTTCTGG CAGAGAGCCG 
GGGGCCAAGC CCAGCACTGC AGAGCCCAGC TCGAGCCCCC AGGACCCTCC CAAGCCTGTT 
GCTGCGCACA GTGAAAGCAG CAGCCACAAG CCCCGGCCTG GCCCTGACCC GGGCCCTCCA 
AAGACTAAGC ACCCCGACCG GTCCCTCTCC TCTCAGAAAC CAAGTGTCGG GGCCACAAAG 
GGCAAAGAGC CTGCCACTCA GTCCCTCGGT GGCTCTAGCA GAGAGGGGAA GGGCCACAGT 
AAGAGTGGGC CGGATGTGTT TCCTGCTACC CCAGGCTCCC AGAACAAAGC CAGCGATGGG 
ATTGGCCAGG GAGAAGGTGG GCCCTCTGTC CCACTGCACA CTGACAGGGC TCCTCTAGAC 
GCCAAGCCAC AACCCACCAG TGGTGGGCGG CCCCTGGAGG TGCTGGAGAA GCCTGTGCAT 
TTGCCAAGGC CGGGACACCC AGGGCCTAGT GAGCCAGCGG ACCAGAAACT GTCCGCTGTT 
GGTGAAAAGC AAACCCTGTC TCCAAAGCAC CCCAAACCAT CCACTGTGAA AGATTGCCCC 
ACCCTGTGCA AACAGACAGA CAACAGACAG ACAGACAAAA GCCCGAGTCA GCCGGCCGCC 
AACACCGACA GAAGGGCGGA AGGGAAGAAA TGCACTGAAG CACTTTATGC TCCAGCAGAG 
GGCGACAAGC TCGAGGCCGG CCTTTCCTTT GTGCATAGCG AGAACCGGTT GAAAGGCGCG 
GAGCGGCCAG CCGCGGGGGT GGGGAAGGGC TTCCCTGAGG CCAGAGGGAA AGGGCCCGGT 
CCCCAGAAGC CACCGACGGA GGCAGACAAG CCCAATGGCA TGAAACGGTC CCCCTCAGCC 
ACTGGGCAGA GTTCTTTCCG ATCCACGGCC CTCCCGGAAA AGTCTCTGAG CTGCTCCTCC 
AGCTTCCCTG AAACCAGGGC CGGAGTTAGA GAGGCCTCTG CAGCCAGCAG CGACACCTCT 
TCTGCCAAGG CCGCCGGGGG CATGCTGGAG CTTCCAGCCC CCAGCAACAG GGACCATAGG 
AAGGCTCAGC CTGCCGGGGA GGGCCGAACC CACATGACAA AGAGTGACTC CCTGCCCTCC 
TTCCGGGTCT CCACCCTGCC TCTGGAGTCA CACCACCCCG ACCCAAACAC CATGGGCGGG 
GCCAGCCACC GGGACAGGGC TCTCTCGGTG ACTGCCACCG TAGGGGAAAC CAAAGGGAAG 
GACCCTGCCC CAGCCCAGCC TCCCCCAGCT AGGAAACAGA ACGTGGGCAG AGACGTGACC 
AAGCCATCCC CAGCCCCAAA CACTGACCGC CCCATCTCTC TTTCTAATGA GAAGGACTTT 
GTGGTACGGC AGAGGCGGGG GAAAGAGAGT TTGCGTAGCA GCCCTCACAA AAAGGCCTTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_017009447.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864936.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.

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CloneID	OHu95868
Clone ID Related Accession (Same CDS sequence)	XM_017009447.1
Accession Version	XM_017009447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8064bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGGAAGGGCA GCCTCCGAAG 
AGCCATTTCA ACTCAGCCCG ACATCGCCAG AGACTAGTGG ACCCAGCTGC TTCAAAAAAG 
GAGCTGAGTC TCCCCAGAAG AGGAAGTTTG ATAGATTCCC AGAAGTGGAA TTGCTTGGTC 
AAACGTCTCA CTCCCAGGAG CCTGAGTCCA ACCCCATCCA GCCCAGGCAG TCCTTGTAGT 
CCTCTCTTGG CCTTTCACTT TTGGAGTTGC CGAACAAGCA ACCGGAAAAG CTTAATAGGC 
AATGGGCAGT CACCAGCATT GCCTCGACCA CACTCACCTC TCTCTGCTCA TGCAGGAAAT 
AGCCCTCAAG ATAGTCCAAG AAATTTCTCC CCCAGTGCCT CAGCCCATTT TTCATTTGCA 
CGGAGGACTG ATGGACGCCG CTGGTCGTTG GCTTCTCTCC CTTCCTCTGG CTATGGGACA 
AACACACCCA GCTCTACGGT CTCTTCATCC TGTTCCTCCC AGGAGAAGTT GCATCAGTTA 
CCATACCAAC CAACACCAGA CGAGTTACAC TTCTTATCAA AACATTTCTG TACCACCGAA 
AGCATCGCCA CTGAGAACAG ATGCAGGAAC ACGCCGATGC GCCCCCGTTC CCGAAGTCTG 
AGCCCTGGAC GTTCTCCCGC CTGCTGTGAC CATGAAATAA TTATGATGAA CCATGTCTAC 
AAAGAAAGGT TCCCAAAGGC TACAGCTCAG ATGGAAGAAC GTCTAAAGGA AATTATCACC 
AGCTACTCTC CTGACAACGT TCTACCCTTA GCAGATGGAG TGCTTAGTTT CACTCACCAC 
CAGATTATTG AACTGGCTCG AGATTGCTTG GATAAATCCC ACCAGGGCCT CATCACCTCA 
CGATACTTCC TTGAATTACA GCACAAATTA GATAAGTTGC TACAGGAGGC TCATGATCGT 
TCAGAAAGTG GAGAATTGGC ATTTATTAAA CAACTAGTTC GAAAGATCCT AATTGTTATT 
GCCCGCCCTG CTCGGTTATT AGAGTGCCTG GAATTTGATC CGGAAGAATT TTACTACCTA 
TTGGAAGCAG CAGAAGGCCA TGCCAAAGAA GGACAGGGTA TTAAAACCGA CATTCCCAGG 
TACATCATTA GCCAACTGGG ACTCAATAAG GATCCCTTGG AAGAAATGGC TCATTTGGGA 
AACTACGATA GTGGGACAGC AGAAACACCA GAAACAGATG AATCAGTGAG TAGCTCTAAT 
GCCTCCCTGA AACTTCGAAG GAAACCTCGG GAAAGTGATT TTGAAACGAT TAAATTGATT 
AGCAATGGAG CCTATGGGGC AGTCTACTTT GTTCGGCATA AAGAATCCCG GCAGAGGTTT 
GCCATGAAGA AGATTAATAA ACAGAACCTC ATCCTTCGAA ACCAGATCCA GCAGGCCTTT 
GTGGAGCGGG ATATCCTGAC TTTTGCAGAA AACCCCTTTG TTGTCAGCAT GTATTGCTCC 
TTTGAAACAA GGCGCCACTT GTGCATGGTC ATGGAATATG TGGAAGGGGG AGACTGTGCT 
ACTTTAATGA AAAACATGGG TCCTCTCCCT GTTGATATGG CCAGAATGTA CTTTGCTGAG 
ACGGTCTTGG CCTTGGAATA TTTACATAAT TATGGAATTG TACACAGGGA TTTGAAACCA 
GACAACTTGT TGGTTACCTC CATGGGGCAC ATAAAGCTGA CAGATTTTGG ATTATCTAAG 
GTGGGACTAA TGAGCATGAC TACCAACCTT TACGAGGGTC ATATTGAGAA GGATGCTAGA 
GAGTTCCTGG ATAAACAGGT CTGTGGCACA CCTGAATACA TTGCACCAGA AGTGATTCTG 
AGGCAGGGTT ATGGAAAGCC GGTGGACTGG TGGGCCATGG GGATTATCCT CTATGAATTT 
CTGGTTGGAT GCGTGCCATT CTTTGGGGAT ACTCCAGAGG AGCTATTTGG ACAAGTCATC 
AGTGATGAGA TCAACTGGCC TGAGAAGGAT GAGGCACCCC CACCTGATGC CCAGGATCTG 
ATTACCTTAC TCCTCAGGCA GAATCCCCTG GAGAGGCTGG GAACAGGTGG TGCATATGAA 
GTCAAACAGC ATCGATTCTT CCGTTCTTTA GACTGGAACA GTTTGCTGAG ACAGAAGGCA 
GAATTTATTC CCCAACTGGA ATCTGAGGAT GACACAAGTT ATTTTGATAC TCGGTCTGAG 
AAGTATCATC ATATGGAAAC GGAGGAAGAA GATGACACAA ATGATGAAGA CTTTAATGTG 
GAAATAAGGC AGTTTTCTTC ATGTTCACAC AGGTTTTCAA AAGTTTTCAG CAGTATAGAT 
CGAATCACTC AGAATTCAGC AGAAGAGAAG GAAGACTCTG TGGACAAAAC CAAAAGCACC 
ACCTTGCCAT CCACAGAAAC ACTGAGCTGG AGTTCAGAAT ATTCTGAAAT GCAACAGCTA 
TCAACATCCA ACTCTTCAGA TACTGAAAGC AACAGACATA AACTCAGTTC TGGCCTACTT 
CCCAAACTGG CTATTTCAAC AGAGGGAGAG CAAGATGAAG CTGCCTCCTG CCCTGGAGAC 
CCCCATGAGG AGCCAGGAAA GCCAGCCCTT CCTCCTGAAG AGTGTGCCCA GGAGGAGCCT 
GAGGTCACCA CCCCAGCCAG CACCATCAGC AGCTCCACCC TGTCAGTTGG CAGTTTTTCA 
GAGCACTTGG ATCAGATAAA TGGACGAAGC GAGTGTGTGG ACAGTACAGA TAATTCCTCA 
AAGCCATCCA GTGAACCCGC TTCTCACATG GCTCGGCAGC GATTAGAAAG CACAGAAAAA 
AAGAAAATCT CGGGGAAAGT CACAAAGTCC CTCTCTGCCA GTGCTCTTTC CCTCATGATC 
CCAGGAGATA TGTTTGCTGT TTCCCCTCTG GGAAGTCCAA TGTCTCCCCA TTCCCTGTCC 
TCGGACCCTT CTTCTTCACG AGATTCCTCT CCCAGCCGAG ATTCCTCAGC AGCTTCTGCC 
AGTCCACATC AGCCGATTGT GATCCACAGT TCGGGGAAGA ACTACGGCTT TACCATCCGA 
GCCATCCGGG TGTATGTGGG AGACAGTGAC ATCTATACAG TGCACCATAT CGTCTGGAAT 
GTAGAAGAAG GAAGTCCGGC ATGCCAGGCA GGACTGAAGG CTGGAGATCT TATCACTCAC 
ATCAATGGAG AACCAGTGCA TGGACTTGTC CACACAGAAG TTATAGAACT CCTACTGAAG 
AGTGGGAATA AGGTGTCAAT CACTACTACC CCATTTGAAA ACACATCAAT CAAAACTGGA 
CCAGCCAGGA GAAACAGCTA TAAGAGCCGG ATGGTGAGGC GGAGCAAGAA ATCCAAGAAG 
AAAGAAAGTC TCGAAAGGAG GAGATCTCTT TTCAAAAAGC TAGCCAAGCA GCCTTCTCCT 
TTACTCCACA CCAGCCGAAG TTTCTCCTGC TTGAACAGAT CCCTGTCATC GGGTGAGAGC 
CTCCCAGGTT CCCCCACTCA TAGCTTGTCT CCCCGGTCTC CAACACCAAG CTACCGCTCC 
ACCCCTGACT TCCCATCTGG TACTAATTCC TCCCAGAGCA GCTCCCCTAG TTCTAGTGCC 
CCCAATTCCC CAGCAGGGTC CGGGCACATC CGGCCCAGCA CTCTCCACGG TCTTGCACCC 
AAACTCGGCG GGCAGCGGTA CCGGTCCGGA AGGCGAAAGT CCGCCGGCAA CATCCCACTG 
TCCCCGCTGG CCCGGACGCC CTCTCCAACC CCGCAACCCA CCTCCCCGCA GCGGTCACCA 
TCCCCTCTTC TGGGACACTC ACTGGGCAAT TCCAAGATCG CGCAAGCCTT TCCCAGCAAG 
ATGCACTCCC CGCCCACCAT CGTCAGACAC ATCGTGAGGC CCAAGAGTGC GGAGCCCCCC 
AGGTCCCCGC TGCTCAAGCG CGTGCAGTCC GAGGAGAAGC TGTCGCCCTC TTACGGCAGT 
GACAAGAAGC ACCTGTGCTC CCGCAAGCAC AGCCTGGAGG TGACCCAAGA GGAGGTGCAG 
CGGGAGCAGT CCCAGCGGGA GGCGCCGCTG CAGAGCCTGG ATGAGAACGT GTGCGACGTG 
CCGCCGCTCA GCCGCGCCCG GCCAGTGGAG CAAGGCTGCC TGAAACGCCC AGTCTCCCGG 
AAGGTGGGCC GCCAGGAGTC TGTGGACGAC CTGGACCGCG ACAAGCTGAA GGCCAAGGTG 
GTGGTGAAGA AAGCAGACGG CTTCCCAGAG AAACAGGAAT CCCACCAGAA ATCCCATGGA 
CCCGGGAGTG ATTTGGAAAA CTTTGCTCTG TTTAAGCTGG AAGAGAGAGA GAAGAAAGTC 
TATCCGAAGG CTGTGGAAAG GTCAAGTACT TTTGAAAACA AAGCGTCTAT GCAGGAGGCG 
CCACCGCTGG GCAGCCTGCT GAAGGATGCT CTTCACAAGC AGGCCAGCGT GCGCGCCAGC 
GAGGGTGCGA TGTCGGATGG CCGGGTGCCT GCGGAGCACC GCCAGGGTGG CGGGGACTTC 
AGACGGGCCC CCGCTCCTGG CACCCTCCAG GATGGTCTCT GCCACTCCCT CGACAGGGGC 
ATCTCTGGGA AGGGGGAAGG CACGGAGAAG TCCTCCCAGG CCAAGGAGCT TCTCCGATGT 
GAAAAGTTAG ACAGCAAGCT GGCCAACATC GATTACCTCC GAAAGAAAAT GTCACTTGAG 
GACAAAGAGG ACAACCTCTG CCCTGTGCTG AAGCCCAAGA TGACAGCTGG CTCCCACGAA 
TGCCTGCCAG GGAACCCAGT CCGACCCACG GGTGGGCAGC AGGAGCCCCC GCCGGCTTCT 
GAGAGCCGAG CTTTTGTCAG CAGCACCCAT GCAGCTCAGA TGAGTGCCGT CTCTTTTGTT 
CCCCTCAAGG CCTTAACAGG CCGGGTGGAC AGTGGAACGG AGAAGCCTGG CTTGGTTGCT 
CCTGAGTCCC CTGTTAGGAA GAGCCCCTCC GAGTATAAGC TGGAAGGTAG GTCTGTCTCA 
TGCCTGAAGC CGATCGAGGG CACTCTGGAC ATTGCTCTCC TGTCCGGACC TCAGGCCTCC 
AAGACAGAAC TGCCTTCCCC AGAGTCTGCA CAGAGCCCCA GCCCAAGTGG TGACGTGAGG 
GCCTCTGTGC CACCAGTTCT CCCCAGCAGC AGTGGGAAAA AGAACGATAC CACCAGTGCA 
AGAGAGCTTT CTCCTTCCAG CTTAAAGATG AATAAATCCT ACCTGCTGGA GCCTTGGTTC 
CTGCCCCCCA GCCGAGGTCT CCAGAATTCA CCAGCAGTTT CCCTGCCTGA CCCAGAGTTC 
AAGAGGGACA GGAAAGGTCC CCATCCTACT GCCAGGAGCC CTGGAACAGT CATGGAAAGC 
AATCCCCAAC AGAGAGAGGG CAGCTCCCCT AAACACCAAG ACCACACCAC TGACCCCAAG 
CTTCTGACCT GCCTGGGGCA GAACCTCCAC AGCCCTGACC TGGCCAGGCC ACGCTGCCCG 
CTCCCACCTG AAGCTTCCCC CTCAAGGGAG AAGCCAGGCC TGAGGGAATC GTCTGAAAGA 
GGCCCTCCCA CAGCCAGAAG CGAGCGCTCT GCTGCGAGGG CTGACACATG CAGAGAGCCC 
TCCATGGAAC TGTGCTTTCC AGAAACTGCG AAAACCAGTG ACAACTCCAA AAATCTCCTC 
TCTGTGGGAA GGACCCACCC AGATTTCTAT ACACAGACCC AGGCCATGGA GAAAGCATGG 
GCGCCGGGTG GGAAAACGAA CCACAAAGAT GGCCCAGGTG AGGCGAGGCC CCCGCCCAGA 
GACAACTCCT CTCTGCACTC AGCTGGAATT CCCTGTGAGA AGGAGCTGGG CAAGGTGAGG 
CGTGGCGTGG AACCCAAGCC CGAAGCGCTT CTTGCCAGGC GGTCTCTGCA GCCACCTGGA 
ATTGAGAGTG AGAAGAGTGA AAAGCTCTCC AGTTTCCCAT CTTTGCAGAA AGATGGTGCC 
AAGGAACCTG AAAGGAAGGA GCAGCCTCTA CAAAGGCATC CCAGCAGCAT CCCTCCGCCC 
CCTCTGACGG CCAAAGACCT GTCCAGCCCG GCTGCCAGGC AGCATTGCAG TTCCCCAAGC 
CACGCTTCTG GCAGAGAGCC GGGGGCCAAG CCCAGCACTG CAGAGCCCAG CTCGAGCCCC 
CAGGACCCTC CCAAGCCTGT TGCTGCGCAC AGTGAAAGCA GCAGCCACAA GCCCCGGCCT 
GGCCCTGACC CGGGCCCTCC AAAGACTAAG CACCCCGACC GGTCCCTCTC CTCTCAGAAA 
CCAAGTGTCG GGGCCACAAA GGGCAAAGAG CCTGCCACTC AGTCCCTCGG TGGCTCTAGC 
AGAGAGGGGA AGGGCCACAG TAAGAGTGGG CCGGATGTGT TTCCTGCTAC CCCAGGCTCC 
CAGAACAAAG CCAGCGATGG GATTGGCCAG GGAGAAGGTG GGCCCTCTGT CCCACTGCAC 
ACTGACAGGG CTCCTCTAGA CGCCAAGCCA CAACCCACCA GTGGTGGGCG GCCCCTGGAG 
GTGCTGGAGA AGCCTGTGCA TTTGCCAAGG CCGGGACACC CAGGGCCTAG TGAGCCAGCG 
GACCAGAAAC TGTCCGCTGT TGGTGAAAAG CAAACCCTGT CTCCAAAGCA CCCCAAACCA 
TCCACTGTGA AAGATTGCCC CACCCTGTGC AAACAGACAG ACAACAGACA GACAGACAAA 
AGCCCGAGTC AGCCGGCCGC CAACACCGAC AGAAGGGCGG AAGGGAAGAA ATGCACTGAA 
GCACTTTATG CTCCAGCAGA GGGCGACAAG CTCGAGGCCG GCCTTTCCTT TGTGCATAGC 
GAGAACCGGT TGAAAGGCGC GGAGCGGCCA GCCGCGGGGG TGGGGAAGGG CTTCCCTGAG 
GCCAGAGGGA AAGGGCCCGG TCCCCAGAAG CCACCGACGG AGGCAGACAA GCCCAATGGC 
ATGAAACGGT CCCCCTCAGC CACTGGGCAG AGTTCTTTCC GATCCACGGC CCTCCCGGAA 
AAGTCTCTGA GCTGCTCCTC CAGCTTCCCT GAAACCAGGG CCGGAGTTAG AGAGGCCTCT 
GCAGCCAGCA GCGACACCTC TTCTGCCAAG GCCGCCGGGG GCATGCTGGA GCTTCCAGCC 
CCCAGCAACA GGGACCATAG GAAGGCTCAG CCTGCCGGGG AGGGCCGAAC CCACATGACA 
AAGAGTGACT CCCTGCCCTC CTTCCGGGTC TCCACCCTGC CTCTGGAGTC ACACCACCCC 
GACCCAAACA CCATGGGCGG GGCCAGCCAC CGGGACAGGG CTCTCTCGGT GACTGCCACC 
GTAGGGGAAA CCAAAGGGAA GGACCCTGCC CCAGCCCAGC CTCCCCCAGC TAGGAAACAG 
AACGTGGGCA GAGACGTGAC CAAGCCATCC CCAGCCCCAA ACACTGACCG CCCCATCTCT 
CTTTCTAATG AGAAGGACTT TGTGGTACGG CAGAGGCGGG GGAAAGAGAG TTTGCGTAGC 
AGCCCTCACA AAAAGGCCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864936.1
CDS	290..8353
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSLIDSQKWNCLVKRLTPRSLSPTPSSPGSPCSPLLAFHFWSCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_017009447.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009447.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGGAAGGGCA GCCTCCGAAG 
AGCCATTTCA ACTCAGCCCG ACATCGCCAG AGACTAGTGG ACCCAGCTGC TTCAAAAAAG 
GAGCTGAGTC TCCCCAGAAG AGGAAGTTTG ATAGATTCCC AGAAGTGGAA TTGCTTGGTC 
AAACGTCTCA CTCCCAGGAG CCTGAGTCCA ACCCCATCCA GCCCAGGCAG TCCTTGTAGT 
CCTCTCTTGG CCTTTCACTT TTGGAGTTGC CGAACAAGCA ACCGGAAAAG CTTAATAGGC 
AATGGGCAGT CACCAGCATT GCCTCGACCA CACTCACCTC TCTCTGCTCA TGCAGGAAAT 
AGCCCTCAAG ATAGTCCAAG AAATTTCTCC CCCAGTGCCT CAGCCCATTT TTCATTTGCA 
CGGAGGACTG ATGGACGCCG CTGGTCGTTG GCTTCTCTCC CTTCCTCTGG CTATGGGACA 
AACACACCCA GCTCTACGGT CTCTTCATCC TGTTCCTCCC AGGAGAAGTT GCATCAGTTA 
CCATACCAAC CAACACCAGA CGAGTTACAC TTCTTATCAA AACATTTCTG TACCACCGAA 
AGCATCGCCA CTGAGAACAG ATGCAGGAAC ACGCCGATGC GCCCCCGTTC CCGAAGTCTG 
AGCCCTGGAC GTTCTCCCGC CTGCTGTGAC CATGAAATAA TTATGATGAA CCATGTCTAC 
AAAGAAAGGT TCCCAAAGGC TACAGCTCAG ATGGAAGAAC GTCTAAAGGA AATTATCACC 
AGCTACTCTC CTGACAACGT TCTACCCTTA GCAGATGGAG TGCTTAGTTT CACTCACCAC 
CAGATTATTG AACTGGCTCG AGATTGCTTG GATAAATCCC ACCAGGGCCT CATCACCTCA 
CGATACTTCC TTGAATTACA GCACAAATTA GATAAGTTGC TACAGGAGGC TCATGATCGT 
TCAGAAAGTG GAGAATTGGC ATTTATTAAA CAACTAGTTC GAAAGATCCT AATTGTTATT 
GCCCGCCCTG CTCGGTTATT AGAGTGCCTG GAATTTGATC CGGAAGAATT TTACTACCTA 
TTGGAAGCAG CAGAAGGCCA TGCCAAAGAA GGACAGGGTA TTAAAACCGA CATTCCCAGG 
TACATCATTA GCCAACTGGG ACTCAATAAG GATCCCTTGG AAGAAATGGC TCATTTGGGA 
AACTACGATA GTGGGACAGC AGAAACACCA GAAACAGATG AATCAGTGAG TAGCTCTAAT 
GCCTCCCTGA AACTTCGAAG GAAACCTCGG GAAAGTGATT TTGAAACGAT TAAATTGATT 
AGCAATGGAG CCTATGGGGC AGTCTACTTT GTTCGGCATA AAGAATCCCG GCAGAGGTTT 
GCCATGAAGA AGATTAATAA ACAGAACCTC ATCCTTCGAA ACCAGATCCA GCAGGCCTTT 
GTGGAGCGGG ATATCCTGAC TTTTGCAGAA AACCCCTTTG TTGTCAGCAT GTATTGCTCC 
TTTGAAACAA GGCGCCACTT GTGCATGGTC ATGGAATATG TGGAAGGGGG AGACTGTGCT 
ACTTTAATGA AAAACATGGG TCCTCTCCCT GTTGATATGG CCAGAATGTA CTTTGCTGAG 
ACGGTCTTGG CCTTGGAATA TTTACATAAT TATGGAATTG TACACAGGGA TTTGAAACCA 
GACAACTTGT TGGTTACCTC CATGGGGCAC ATAAAGCTGA CAGATTTTGG ATTATCTAAG 
GTGGGACTAA TGAGCATGAC TACCAACCTT TACGAGGGTC ATATTGAGAA GGATGCTAGA 
GAGTTCCTGG ATAAACAGGT CTGTGGCACA CCTGAATACA TTGCACCAGA AGTGATTCTG 
AGGCAGGGTT ATGGAAAGCC GGTGGACTGG TGGGCCATGG GGATTATCCT CTATGAATTT 
CTGGTTGGAT GCGTGCCATT CTTTGGGGAT ACTCCAGAGG AGCTATTTGG ACAAGTCATC 
AGTGATGAGA TCAACTGGCC TGAGAAGGAT GAGGCACCCC CACCTGATGC CCAGGATCTG 
ATTACCTTAC TCCTCAGGCA GAATCCCCTG GAGAGGCTGG GAACAGGTGG TGCATATGAA 
GTCAAACAGC ATCGATTCTT CCGTTCTTTA GACTGGAACA GTTTGCTGAG ACAGAAGGCA 
GAATTTATTC CCCAACTGGA ATCTGAGGAT GACACAAGTT ATTTTGATAC TCGGTCTGAG 
AAGTATCATC ATATGGAAAC GGAGGAAGAA GATGACACAA ATGATGAAGA CTTTAATGTG 
GAAATAAGGC AGTTTTCTTC ATGTTCACAC AGGTTTTCAA AAGTTTTCAG CAGTATAGAT 
CGAATCACTC AGAATTCAGC AGAAGAGAAG GAAGACTCTG TGGACAAAAC CAAAAGCACC 
ACCTTGCCAT CCACAGAAAC ACTGAGCTGG AGTTCAGAAT ATTCTGAAAT GCAACAGCTA 
TCAACATCCA ACTCTTCAGA TACTGAAAGC AACAGACATA AACTCAGTTC TGGCCTACTT 
CCCAAACTGG CTATTTCAAC AGAGGGAGAG CAAGATGAAG CTGCCTCCTG CCCTGGAGAC 
CCCCATGAGG AGCCAGGAAA GCCAGCCCTT CCTCCTGAAG AGTGTGCCCA GGAGGAGCCT 
GAGGTCACCA CCCCAGCCAG CACCATCAGC AGCTCCACCC TGTCAGTTGG CAGTTTTTCA 
GAGCACTTGG ATCAGATAAA TGGACGAAGC GAGTGTGTGG ACAGTACAGA TAATTCCTCA 
AAGCCATCCA GTGAACCCGC TTCTCACATG GCTCGGCAGC GATTAGAAAG CACAGAAAAA 
AAGAAAATCT CGGGGAAAGT CACAAAGTCC CTCTCTGCCA GTGCTCTTTC CCTCATGATC 
CCAGGAGATA TGTTTGCTGT TTCCCCTCTG GGAAGTCCAA TGTCTCCCCA TTCCCTGTCC 
TCGGACCCTT CTTCTTCACG AGATTCCTCT CCCAGCCGAG ATTCCTCAGC AGCTTCTGCC 
AGTCCACATC AGCCGATTGT GATCCACAGT TCGGGGAAGA ACTACGGCTT TACCATCCGA 
GCCATCCGGG TGTATGTGGG AGACAGTGAC ATCTATACAG TGCACCATAT CGTCTGGAAT 
GTAGAAGAAG GAAGTCCGGC ATGCCAGGCA GGACTGAAGG CTGGAGATCT TATCACTCAC 
ATCAATGGAG AACCAGTGCA TGGACTTGTC CACACAGAAG TTATAGAACT CCTACTGAAG 
AGTGGGAATA AGGTGTCAAT CACTACTACC CCATTTGAAA ACACATCAAT CAAAACTGGA 
CCAGCCAGGA GAAACAGCTA TAAGAGCCGG ATGGTGAGGC GGAGCAAGAA ATCCAAGAAG 
AAAGAAAGTC TCGAAAGGAG GAGATCTCTT TTCAAAAAGC TAGCCAAGCA GCCTTCTCCT 
TTACTCCACA CCAGCCGAAG TTTCTCCTGC TTGAACAGAT CCCTGTCATC GGGTGAGAGC 
CTCCCAGGTT CCCCCACTCA TAGCTTGTCT CCCCGGTCTC CAACACCAAG CTACCGCTCC 
ACCCCTGACT TCCCATCTGG TACTAATTCC TCCCAGAGCA GCTCCCCTAG TTCTAGTGCC 
CCCAATTCCC CAGCAGGGTC CGGGCACATC CGGCCCAGCA CTCTCCACGG TCTTGCACCC 
AAACTCGGCG GGCAGCGGTA CCGGTCCGGA AGGCGAAAGT CCGCCGGCAA CATCCCACTG 
TCCCCGCTGG CCCGGACGCC CTCTCCAACC CCGCAACCCA CCTCCCCGCA GCGGTCACCA 
TCCCCTCTTC TGGGACACTC ACTGGGCAAT TCCAAGATCG CGCAAGCCTT TCCCAGCAAG 
ATGCACTCCC CGCCCACCAT CGTCAGACAC ATCGTGAGGC CCAAGAGTGC GGAGCCCCCC 
AGGTCCCCGC TGCTCAAGCG CGTGCAGTCC GAGGAGAAGC TGTCGCCCTC TTACGGCAGT 
GACAAGAAGC ACCTGTGCTC CCGCAAGCAC AGCCTGGAGG TGACCCAAGA GGAGGTGCAG 
CGGGAGCAGT CCCAGCGGGA GGCGCCGCTG CAGAGCCTGG ATGAGAACGT GTGCGACGTG 
CCGCCGCTCA GCCGCGCCCG GCCAGTGGAG CAAGGCTGCC TGAAACGCCC AGTCTCCCGG 
AAGGTGGGCC GCCAGGAGTC TGTGGACGAC CTGGACCGCG ACAAGCTGAA GGCCAAGGTG 
GTGGTGAAGA AAGCAGACGG CTTCCCAGAG AAACAGGAAT CCCACCAGAA ATCCCATGGA 
CCCGGGAGTG ATTTGGAAAA CTTTGCTCTG TTTAAGCTGG AAGAGAGAGA GAAGAAAGTC 
TATCCGAAGG CTGTGGAAAG GTCAAGTACT TTTGAAAACA AAGCGTCTAT GCAGGAGGCG 
CCACCGCTGG GCAGCCTGCT GAAGGATGCT CTTCACAAGC AGGCCAGCGT GCGCGCCAGC 
GAGGGTGCGA TGTCGGATGG CCGGGTGCCT GCGGAGCACC GCCAGGGTGG CGGGGACTTC 
AGACGGGCCC CCGCTCCTGG CACCCTCCAG GATGGTCTCT GCCACTCCCT CGACAGGGGC 
ATCTCTGGGA AGGGGGAAGG CACGGAGAAG TCCTCCCAGG CCAAGGAGCT TCTCCGATGT 
GAAAAGTTAG ACAGCAAGCT GGCCAACATC GATTACCTCC GAAAGAAAAT GTCACTTGAG 
GACAAAGAGG ACAACCTCTG CCCTGTGCTG AAGCCCAAGA TGACAGCTGG CTCCCACGAA 
TGCCTGCCAG GGAACCCAGT CCGACCCACG GGTGGGCAGC AGGAGCCCCC GCCGGCTTCT 
GAGAGCCGAG CTTTTGTCAG CAGCACCCAT GCAGCTCAGA TGAGTGCCGT CTCTTTTGTT 
CCCCTCAAGG CCTTAACAGG CCGGGTGGAC AGTGGAACGG AGAAGCCTGG CTTGGTTGCT 
CCTGAGTCCC CTGTTAGGAA GAGCCCCTCC GAGTATAAGC TGGAAGGTAG GTCTGTCTCA 
TGCCTGAAGC CGATCGAGGG CACTCTGGAC ATTGCTCTCC TGTCCGGACC TCAGGCCTCC 
AAGACAGAAC TGCCTTCCCC AGAGTCTGCA CAGAGCCCCA GCCCAAGTGG TGACGTGAGG 
GCCTCTGTGC CACCAGTTCT CCCCAGCAGC AGTGGGAAAA AGAACGATAC CACCAGTGCA 
AGAGAGCTTT CTCCTTCCAG CTTAAAGATG AATAAATCCT ACCTGCTGGA GCCTTGGTTC 
CTGCCCCCCA GCCGAGGTCT CCAGAATTCA CCAGCAGTTT CCCTGCCTGA CCCAGAGTTC 
AAGAGGGACA GGAAAGGTCC CCATCCTACT GCCAGGAGCC CTGGAACAGT CATGGAAAGC 
AATCCCCAAC AGAGAGAGGG CAGCTCCCCT AAACACCAAG ACCACACCAC TGACCCCAAG 
CTTCTGACCT GCCTGGGGCA GAACCTCCAC AGCCCTGACC TGGCCAGGCC ACGCTGCCCG 
CTCCCACCTG AAGCTTCCCC CTCAAGGGAG AAGCCAGGCC TGAGGGAATC GTCTGAAAGA 
GGCCCTCCCA CAGCCAGAAG CGAGCGCTCT GCTGCGAGGG CTGACACATG CAGAGAGCCC 
TCCATGGAAC TGTGCTTTCC AGAAACTGCG AAAACCAGTG ACAACTCCAA AAATCTCCTC 
TCTGTGGGAA GGACCCACCC AGATTTCTAT ACACAGACCC AGGCCATGGA GAAAGCATGG 
GCGCCGGGTG GGAAAACGAA CCACAAAGAT GGCCCAGGTG AGGCGAGGCC CCCGCCCAGA 
GACAACTCCT CTCTGCACTC AGCTGGAATT CCCTGTGAGA AGGAGCTGGG CAAGGTGAGG 
CGTGGCGTGG AACCCAAGCC CGAAGCGCTT CTTGCCAGGC GGTCTCTGCA GCCACCTGGA 
ATTGAGAGTG AGAAGAGTGA AAAGCTCTCC AGTTTCCCAT CTTTGCAGAA AGATGGTGCC 
AAGGAACCTG AAAGGAAGGA GCAGCCTCTA CAAAGGCATC CCAGCAGCAT CCCTCCGCCC 
CCTCTGACGG CCAAAGACCT GTCCAGCCCG GCTGCCAGGC AGCATTGCAG TTCCCCAAGC 
CACGCTTCTG GCAGAGAGCC GGGGGCCAAG CCCAGCACTG CAGAGCCCAG CTCGAGCCCC 
CAGGACCCTC CCAAGCCTGT TGCTGCGCAC AGTGAAAGCA GCAGCCACAA GCCCCGGCCT 
GGCCCTGACC CGGGCCCTCC AAAGACTAAG CACCCCGACC GGTCCCTCTC CTCTCAGAAA 
CCAAGTGTCG GGGCCACAAA GGGCAAAGAG CCTGCCACTC AGTCCCTCGG TGGCTCTAGC 
AGAGAGGGGA AGGGCCACAG TAAGAGTGGG CCGGATGTGT TTCCTGCTAC CCCAGGCTCC 
CAGAACAAAG CCAGCGATGG GATTGGCCAG GGAGAAGGTG GGCCCTCTGT CCCACTGCAC 
ACTGACAGGG CTCCTCTAGA CGCCAAGCCA CAACCCACCA GTGGTGGGCG GCCCCTGGAG 
GTGCTGGAGA AGCCTGTGCA TTTGCCAAGG CCGGGACACC CAGGGCCTAG TGAGCCAGCG 
GACCAGAAAC TGTCCGCTGT TGGTGAAAAG CAAACCCTGT CTCCAAAGCA CCCCAAACCA 
TCCACTGTGA AAGATTGCCC CACCCTGTGC AAACAGACAG ACAACAGACA GACAGACAAA 
AGCCCGAGTC AGCCGGCCGC CAACACCGAC AGAAGGGCGG AAGGGAAGAA ATGCACTGAA 
GCACTTTATG CTCCAGCAGA GGGCGACAAG CTCGAGGCCG GCCTTTCCTT TGTGCATAGC 
GAGAACCGGT TGAAAGGCGC GGAGCGGCCA GCCGCGGGGG TGGGGAAGGG CTTCCCTGAG 
GCCAGAGGGA AAGGGCCCGG TCCCCAGAAG CCACCGACGG AGGCAGACAA GCCCAATGGC 
ATGAAACGGT CCCCCTCAGC CACTGGGCAG AGTTCTTTCC GATCCACGGC CCTCCCGGAA 
AAGTCTCTGA GCTGCTCCTC CAGCTTCCCT GAAACCAGGG CCGGAGTTAG AGAGGCCTCT 
GCAGCCAGCA GCGACACCTC TTCTGCCAAG GCCGCCGGGG GCATGCTGGA GCTTCCAGCC 
CCCAGCAACA GGGACCATAG GAAGGCTCAG CCTGCCGGGG AGGGCCGAAC CCACATGACA 
AAGAGTGACT CCCTGCCCTC CTTCCGGGTC TCCACCCTGC CTCTGGAGTC ACACCACCCC 
GACCCAAACA CCATGGGCGG GGCCAGCCAC CGGGACAGGG CTCTCTCGGT GACTGCCACC 
GTAGGGGAAA CCAAAGGGAA GGACCCTGCC CCAGCCCAGC CTCCCCCAGC TAGGAAACAG 
AACGTGGGCA GAGACGTGAC CAAGCCATCC CCAGCCCCAA ACACTGACCG CCCCATCTCT 
CTTTCTAATG AGAAGGACTT TGTGGTACGG CAGAGGCGGG GGAAAGAGAG TTTGCGTAGC 
AGCCCTCACA AAAAGGCCTT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MAST4 ( XM_017009448.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864937.1 Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA.

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CloneID	OHu95869
Clone ID Related Accession (Same CDS sequence)	XM_017009448.1
Accession Version	XM_017009448.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7947bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	microtubule-associated serine/threonine-protein kinase 4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGGAAGGGCA GCCTCCGAAG 
AGCCATTTCA ACTCAGCCCG ACATCGCCAG AGACTAGTGG ACCCAGCTGC TTCAAAAAAG 
GAGCTGAGTC TCCCCAGAAG AGGAAGTTTT TGCCGAACAA GCAACCGGAA AAGCTTAATA 
GGCAATGGGC AGTCACCAGC ATTGCCTCGA CCACACTCAC CTCTCTCTGC TCATGCAGGA 
AATAGCCCTC AAGATAGTCC AAGAAATTTC TCCCCCAGTG CCTCAGCCCA TTTTTCATTT 
GCACGGAGGA CTGATGGACG CCGCTGGTCG TTGGCTTCTC TCCCTTCCTC TGGCTATGGG 
ACAAACACAC CCAGCTCTAC GGTCTCTTCA TCCTGTTCCT CCCAGGAGAA GTTGCATCAG 
TTACCATACC AACCAACACC AGACGAGTTA CACTTCTTAT CAAAACATTT CTGTACCACC 
GAAAGCATCG CCACTGAGAA CAGATGCAGG AACACGCCGA TGCGCCCCCG TTCCCGAAGT 
CTGAGCCCTG GACGTTCTCC CGCCTGCTGT GACCATGAAA TAATTATGAT GAACCATGTC 
TACAAAGAAA GGTTCCCAAA GGCTACAGCT CAGATGGAAG AACGTCTAAA GGAAATTATC 
ACCAGCTACT CTCCTGACAA CGTTCTACCC TTAGCAGATG GAGTGCTTAG TTTCACTCAC 
CACCAGATTA TTGAACTGGC TCGAGATTGC TTGGATAAAT CCCACCAGGG CCTCATCACC 
TCACGATACT TCCTTGAATT ACAGCACAAA TTAGATAAGT TGCTACAGGA GGCTCATGAT 
CGTTCAGAAA GTGGAGAATT GGCATTTATT AAACAACTAG TTCGAAAGAT CCTAATTGTT 
ATTGCCCGCC CTGCTCGGTT ATTAGAGTGC CTGGAATTTG ATCCGGAAGA ATTTTACTAC 
CTATTGGAAG CAGCAGAAGG CCATGCCAAA GAAGGACAGG GTATTAAAAC CGACATTCCC 
AGGTACATCA TTAGCCAACT GGGACTCAAT AAGGATCCCT TGGAAGAAAT GGCTCATTTG 
GGAAACTACG ATAGTGGGAC AGCAGAAACA CCAGAAACAG ATGAATCAGT GAGTAGCTCT 
AATGCCTCCC TGAAACTTCG AAGGAAACCT CGGGAAAGTG ATTTTGAAAC GATTAAATTG 
ATTAGCAATG GAGCCTATGG GGCAGTCTAC TTTGTTCGGC ATAAAGAATC CCGGCAGAGG 
TTTGCCATGA AGAAGATTAA TAAACAGAAC CTCATCCTTC GAAACCAGAT CCAGCAGGCC 
TTTGTGGAGC GGGATATCCT GACTTTTGCA GAAAACCCCT TTGTTGTCAG CATGTATTGC 
TCCTTTGAAA CAAGGCGCCA CTTGTGCATG GTCATGGAAT ATGTGGAAGG GGGAGACTGT 
GCTACTTTAA TGAAAAACAT GGGTCCTCTC CCTGTTGATA TGGCCAGAAT GTACTTTGCT 
GAGACGGTCT TGGCCTTGGA ATATTTACAT AATTATGGAA TTGTACACAG GGATTTGAAA 
CCAGACAACT TGTTGGTTAC CTCCATGGGG CACATAAAGC TGACAGATTT TGGATTATCT 
AAGGTGGGAC TAATGAGCAT GACTACCAAC CTTTACGAGG GTCATATTGA GAAGGATGCT 
AGAGAGTTCC TGGATAAACA GGTCTGTGGC ACACCTGAAT ACATTGCACC AGAAGTGATT 
CTGAGGCAGG GTTATGGAAA GCCGGTGGAC TGGTGGGCCA TGGGGATTAT CCTCTATGAA 
TTTCTGGTTG GATGCGTGCC ATTCTTTGGG GATACTCCAG AGGAGCTATT TGGACAAGTC 
ATCAGTGATG AGATCAACTG GCCTGAGAAG GATGAGGCAC CCCCACCTGA TGCCCAGGAT 
CTGATTACCT TACTCCTCAG GCAGAATCCC CTGGAGAGGC TGGGAACAGG TGGTGCATAT 
GAAGTCAAAC AGCATCGATT CTTCCGTTCT TTAGACTGGA ACAGTTTGCT GAGACAGAAG 
GCAGAATTTA TTCCCCAACT GGAATCTGAG GATGACACAA GTTATTTTGA TACTCGGTCT 
GAGAAGTATC ATCATATGGA AACGGAGGAA GAAGATGACA CAAATGATGA AGACTTTAAT 
GTGGAAATAA GGCAGTTTTC TTCATGTTCA CACAGGTTTT CAAAAGTTTT CAGCAGTATA 
GATCGAATCA CTCAGAATTC AGCAGAAGAG AAGGAAGACT CTGTGGACAA AACCAAAAGC 
ACCACCTTGC CATCCACAGA AACACTGAGC TGGAGTTCAG AATATTCTGA AATGCAACAG 
CTATCAACAT CCAACTCTTC AGATACTGAA AGCAACAGAC ATAAACTCAG TTCTGGCCTA 
CTTCCCAAAC TGGCTATTTC AACAGAGGGA GAGCAAGATG AAGCTGCCTC CTGCCCTGGA 
GACCCCCATG AGGAGCCAGG AAAGCCAGCC CTTCCTCCTG AAGAGTGTGC CCAGGAGGAG 
CCTGAGGTCA CCACCCCAGC CAGCACCATC AGCAGCTCCA CCCTGTCAGT TGGCAGTTTT 
TCAGAGCACT TGGATCAGAT AAATGGACGA AGCGAGTGTG TGGACAGTAC AGATAATTCC 
TCAAAGCCAT CCAGTGAACC CGCTTCTCAC ATGGCTCGGC AGCGATTAGA AAGCACAGAA 
AAAAAGAAAA TCTCGGGGAA AGTCACAAAG TCCCTCTCTG CCAGTGCTCT TTCCCTCATG 
ATCCCAGGAG ATATGTTTGC TGTTTCCCCT CTGGGAAGTC CAATGTCTCC CCATTCCCTG 
TCCTCGGACC CTTCTTCTTC ACGAGATTCC TCTCCCAGCC GAGATTCCTC AGCAGCTTCT 
GCCAGTCCAC ATCAGCCGAT TGTGATCCAC AGTTCGGGGA AGAACTACGG CTTTACCATC 
CGAGCCATCC GGGTGTATGT GGGAGACAGT GACATCTATA CAGTGCACCA TATCGTCTGG 
AATGTAGAAG AAGGAAGTCC GGCATGCCAG GCAGGACTGA AGGCTGGAGA TCTTATCACT 
CACATCAATG GAGAACCAGT GCATGGACTT GTCCACACAG AAGTTATAGA ACTCCTACTG 
AAGAGTGGGA ATAAGGTGTC AATCACTACT ACCCCATTTG AAAACACATC AATCAAAACT 
GGACCAGCCA GGAGAAACAG CTATAAGAGC CGGATGGTGA GGCGGAGCAA GAAATCCAAG 
AAGAAAGAAA GTCTCGAAAG GAGGAGATCT CTTTTCAAAA AGCTAGCCAA GCAGCCTTCT 
CCTTTACTCC ACACCAGCCG AAGTTTCTCC TGCTTGAACA GATCCCTGTC ATCGGGTGAG 
AGCCTCCCAG GTTCCCCCAC TCATAGCTTG TCTCCCCGGT CTCCAACACC AAGCTACCGC 
TCCACCCCTG ACTTCCCATC TGGTACTAAT TCCTCCCAGA GCAGCTCCCC TAGTTCTAGT 
GCCCCCAATT CCCCAGCAGG GTCCGGGCAC ATCCGGCCCA GCACTCTCCA CGGTCTTGCA 
CCCAAACTCG GCGGGCAGCG GTACCGGTCC GGAAGGCGAA AGTCCGCCGG CAACATCCCA 
CTGTCCCCGC TGGCCCGGAC GCCCTCTCCA ACCCCGCAAC CCACCTCCCC GCAGCGGTCA 
CCATCCCCTC TTCTGGGACA CTCACTGGGC AATTCCAAGA TCGCGCAAGC CTTTCCCAGC 
AAGATGCACT CCCCGCCCAC CATCGTCAGA CACATCGTGA GGCCCAAGAG TGCGGAGCCC 
CCCAGGTCCC CGCTGCTCAA GCGCGTGCAG TCCGAGGAGA AGCTGTCGCC CTCTTACGGC 
AGTGACAAGA AGCACCTGTG CTCCCGCAAG CACAGCCTGG AGGTGACCCA AGAGGAGGTG 
CAGCGGGAGC AGTCCCAGCG GGAGGCGCCG CTGCAGAGCC TGGATGAGAA CGTGTGCGAC 
GTGCCGCCGC TCAGCCGCGC CCGGCCAGTG GAGCAAGGCT GCCTGAAACG CCCAGTCTCC 
CGGAAGGTGG GCCGCCAGGA GTCTGTGGAC GACCTGGACC GCGACAAGCT GAAGGCCAAG 
GTGGTGGTGA AGAAAGCAGA CGGCTTCCCA GAGAAACAGG AATCCCACCA GAAATCCCAT 
GGACCCGGGA GTGATTTGGA AAACTTTGCT CTGTTTAAGC TGGAAGAGAG AGAGAAGAAA 
GTCTATCCGA AGGCTGTGGA AAGGTCAAGT ACTTTTGAAA ACAAAGCGTC TATGCAGGAG 
GCGCCACCGC TGGGCAGCCT GCTGAAGGAT GCTCTTCACA AGCAGGCCAG CGTGCGCGCC 
AGCGAGGGTG CGATGTCGGA TGGCCGGGTG CCTGCGGAGC ACCGCCAGGG TGGCGGGGAC 
TTCAGACGGG CCCCCGCTCC TGGCACCCTC CAGGATGGTC TCTGCCACTC CCTCGACAGG 
GGCATCTCTG GGAAGGGGGA AGGCACGGAG AAGTCCTCCC AGGCCAAGGA GCTTCTCCGA 
TGTGAAAAGT TAGACAGCAA GCTGGCCAAC ATCGATTACC TCCGAAAGAA AATGTCACTT 
GAGGACAAAG AGGACAACCT CTGCCCTGTG CTGAAGCCCA AGATGACAGC TGGCTCCCAC 
GAATGCCTGC CAGGGAACCC AGTCCGACCC ACGGGTGGGC AGCAGGAGCC CCCGCCGGCT 
TCTGAGAGCC GAGCTTTTGT CAGCAGCACC CATGCAGCTC AGATGAGTGC CGTCTCTTTT 
GTTCCCCTCA AGGCCTTAAC AGGCCGGGTG GACAGTGGAA CGGAGAAGCC TGGCTTGGTT 
GCTCCTGAGT CCCCTGTTAG GAAGAGCCCC TCCGAGTATA AGCTGGAAGG TAGGTCTGTC 
TCATGCCTGA AGCCGATCGA GGGCACTCTG GACATTGCTC TCCTGTCCGG ACCTCAGGCC 
TCCAAGACAG AACTGCCTTC CCCAGAGTCT GCACAGAGCC CCAGCCCAAG TGGTGACGTG 
AGGGCCTCTG TGCCACCAGT TCTCCCCAGC AGCAGTGGGA AAAAGAACGA TACCACCAGT 
GCAAGAGAGC TTTCTCCTTC CAGCTTAAAG ATGAATAAAT CCTACCTGCT GGAGCCTTGG 
TTCCTGCCCC CCAGCCGAGG TCTCCAGAAT TCACCAGCAG TTTCCCTGCC TGACCCAGAG 
TTCAAGAGGG ACAGGAAAGG TCCCCATCCT ACTGCCAGGA GCCCTGGAAC AGTCATGGAA 
AGCAATCCCC AACAGAGAGA GGGCAGCTCC CCTAAACACC AAGACCACAC CACTGACCCC 
AAGCTTCTGA CCTGCCTGGG GCAGAACCTC CACAGCCCTG ACCTGGCCAG GCCACGCTGC 
CCGCTCCCAC CTGAAGCTTC CCCCTCAAGG GAGAAGCCAG GCCTGAGGGA ATCGTCTGAA 
AGAGGCCCTC CCACAGCCAG AAGCGAGCGC TCTGCTGCGA GGGCTGACAC ATGCAGAGAG 
CCCTCCATGG AACTGTGCTT TCCAGAAACT GCGAAAACCA GTGACAACTC CAAAAATCTC 
CTCTCTGTGG GAAGGACCCA CCCAGATTTC TATACACAGA CCCAGGCCAT GGAGAAAGCA 
TGGGCGCCGG GTGGGAAAAC GAACCACAAA GATGGCCCAG GTGAGGCGAG GCCCCCGCCC 
AGAGACAACT CCTCTCTGCA CTCAGCTGGA ATTCCCTGTG AGAAGGAGCT GGGCAAGGTG 
AGGCGTGGCG TGGAACCCAA GCCCGAAGCG CTTCTTGCCA GGCGGTCTCT GCAGCCACCT 
GGAATTGAGA GTGAGAAGAG TGAAAAGCTC TCCAGTTTCC CATCTTTGCA GAAAGATGGT 
GCCAAGGAAC CTGAAAGGAA GGAGCAGCCT CTACAAAGGC ATCCCAGCAG CATCCCTCCG 
CCCCCTCTGA CGGCCAAAGA CCTGTCCAGC CCGGCTGCCA GGCAGCATTG CAGTTCCCCA 
AGCCACGCTT CTGGCAGAGA GCCGGGGGCC AAGCCCAGCA CTGCAGAGCC CAGCTCGAGC 
CCCCAGGACC CTCCCAAGCC TGTTGCTGCG CACAGTGAAA GCAGCAGCCA CAAGCCCCGG 
CCTGGCCCTG ACCCGGGCCC TCCAAAGACT AAGCACCCCG ACCGGTCCCT CTCCTCTCAG 
AAACCAAGTG TCGGGGCCAC AAAGGGCAAA GAGCCTGCCA CTCAGTCCCT CGGTGGCTCT 
AGCAGAGAGG GGAAGGGCCA CAGTAAGAGT GGGCCGGATG TGTTTCCTGC TACCCCAGGC 
TCCCAGAACA AAGCCAGCGA TGGGATTGGC CAGGGAGAAG GTGGGCCCTC TGTCCCACTG 
CACACTGACA GGGCTCCTCT AGACGCCAAG CCACAACCCA CCAGTGGTGG GCGGCCCCTG 
GAGGTGCTGG AGAAGCCTGT GCATTTGCCA AGGCCGGGAC ACCCAGGGCC TAGTGAGCCA 
GCGGACCAGA AACTGTCCGC TGTTGGTGAA AAGCAAACCC TGTCTCCAAA GCACCCCAAA 
CCATCCACTG TGAAAGATTG CCCCACCCTG TGCAAACAGA CAGACAACAG ACAGACAGAC 
AAAAGCCCGA GTCAGCCGGC CGCCAACACC GACAGAAGGG CGGAAGGGAA GAAATGCACT 
GAAGCACTTT ATGCTCCAGC AGAGGGCGAC AAGCTCGAGG CCGGCCTTTC CTTTGTGCAT 
AGCGAGAACC GGTTGAAAGG CGCGGAGCGG CCAGCCGCGG GGGTGGGGAA GGGCTTCCCT 
GAGGCCAGAG GGAAAGGGCC CGGTCCCCAG AAGCCACCGA CGGAGGCAGA CAAGCCCAAT 
GGCATGAAAC GGTCCCCCTC AGCCACTGGG CAGAGTTCTT TCCGATCCAC GGCCCTCCCG 
GAAAAGTCTC TGAGCTGCTC CTCCAGCTTC CCTGAAACCA GGGCCGGAGT TAGAGAGGCC 
TCTGCAGCCA GCAGCGACAC CTCTTCTGCC AAGGCCGCCG GGGGCATGCT GGAGCTTCCA 
GCCCCCAGCA ACAGGGACCA TAGGAAGGCT CAGCCTGCCG GGGAGGGCCG AACCCACATG 
ACAAAGAGTG ACTCCCTGCC CTCCTTCCGG GTCTCCACCC TGCCTCTGGA GTCACACCAC 
CCCGACCCAA ACACCATGGG CGGGGCCAGC CACCGGGACA GGGCTCTCTC GGTGACTGCC 
ACCGTAGGGG AAACCAAAGG GAAGGACCCT GCCCCAGCCC AGCCTCCCCC AGCTAGGAAA 
CAGAACGTGG GCAGAGACGT GACCAAGCCA TCCCCAGCCC CAAACACTGA CCGCCCCATC 
TCTCTTTCTA ATGAGAAGGA CTTTGTGGTA CGGCAGAGGC GGGGGAAAGA GAGTTTGCGT 
AGCAGCCCTC ACAAAAAGGC CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864937.1
CDS	290..8236
Translation	MGEKVSEAPEPVPRGCSGHGSRTPASALVAASSPGASSAESSSGSETLSEEGEPGGFSREHQPPPPPPLGGTLGARAPAAWAPASVLLERGVLALPPPLPGGAVPPAPRGSSASQEEQDEELDHILSPPPMPFRKCSNPDVASGPGKSLKYKRQLSEDGRQLRRGSLGGALTGRYLLPNPVAGQAWPASAETSNLVRMRSQALGQSAPSLTASLEGQPPKSHFNSARHRQRLVDPAASKKELSLPRRGSFCRTSNRKSLIGNGQSPALPRPHSPLSAHAGNSPQDSPRNFSPSASAHFSFARRTDGRRWSLASLPSSGYGTNTPSSTVSSSCSSQEKLHQLPYQPTPDELHFLSKHFCTTESIATENRCRNTPMRPRSRSLSPGRSPACCDHEIIMMNHVYKERFPKATAQMEERLKEIITSYSPDNVLPLADGVLSFTHHQIIELARDCLDKSHQGLITSRYFLELQHKLDKLLQEAHDRSESGELAFIKQLVRKILIVIARPARLLECLEFDPEEFYYLLEAAEGHAKEGQGIKTDIPRYIISQLGLNKDPLEEMAHLGNYDSGTAETPETDESVSSSNASLKLRRKPRESDFETIKLISNGAYGAVYFVRHKESRQRFAMKKINKQNLILRNQIQQAFVERDILTFAENPFVVSMYCSFETRRHLCMVMEYVEGGDCATLMKNMGPLPVDMARMYFAETVLALEYLHNYGIVHRDLKPDNLLVTSMGHIKLTDFGLSKVGLMSMTTNLYEGHIEKDAREFLDKQVCGTPEYIAPEVILRQGYGKPVDWWAMGIILYEFLVGCVPFFGDTPEELFGQVISDEINWPEKDEAPPPDAQDLITLLLRQNPLERLGTGGAYEVKQHRFFRSLDWNSLLRQKAEFIPQLESEDDTSYFDTRSEKYHHMETEEEDDTNDEDFNVEIRQFSSCSHRFSKVFSSIDRITQNSAEEKEDSVDKTKSTTLPSTETLSWSSEYSEMQQLSTSNSSDTESNRHKLSSGLLPKLAISTEGEQDEAASCPGDPHEEPGKPALPPEECAQEEPEVTTPASTISSSTLSVGSFSEHLDQINGRSECVDSTDNSSKPSSEPASHMARQRLESTEKKKISGKVTKSLSASALSLMIPGDMFAVSPLGSPMSPHSLSSDPSSSRDSSPSRDSSAASASPHQPIVIHSSGKNYGFTIRAIRVYVGDSDIYTVHHIVWNVEEGSPACQAGLKAGDLITHINGEPVHGLVHTEVIELLLKSGNKVSITTTPFENTSIKTGPARRNSYKSRMVRRSKKSKKKESLERRRSLFKKLAKQPSPLLHTSRSFSCLNRSLSSGESLPGSPTHSLSPRSPTPSYRSTPDFPSGTNSSQSSSPSSSAPNSPAGSGHIRPSTLHGLAPKLGGQRYRSGRRKSAGNIPLSPLARTPSPTPQPTSPQRSPSPLLGHSLGNSKIAQAFPSKMHSPPTIVRHIVRPKSAEPPRSPLLKRVQSEEKLSPSYGSDKKHLCSRKHSLEVTQEEVQREQSQREAPLQSLDENVCDVPPLSRARPVEQGCLKRPVSRKVGRQESVDDLDRDKLKAKVVVKKADGFPEKQESHQKSHGPGSDLENFALFKLEEREKKVYPKAVERSSTFENKASMQEAPPLGSLLKDALHKQASVRASEGAMSDGRVPAEHRQGGGDFRRAPAPGTLQDGLCHSLDRGISGKGEGTEKSSQAKELLRCEKLDSKLANIDYLRKKMSLEDKEDNLCPVLKPKMTAGSHECLPGNPVRPTGGQQEPPPASESRAFVSSTHAAQMSAVSFVPLKALTGRVDSGTEKPGLVAPESPVRKSPSEYKLEGRSVSCLKPIEGTLDIALLSGPQASKTELPSPESAQSPSPSGDVRASVPPVLPSSSGKKNDTTSARELSPSSLKMNKSYLLEPWFLPPSRGLQNSPAVSLPDPEFKRDRKGPHPTARSPGTVMESNPQQREGSSPKHQDHTTDPKLLTCLGQNLHSPDLARPRCPLPPEASPSREKPGLRESSERGPPTARSERSAARADTCREPSMELCFPETAKTSDNSKNLLSVGRTHPDFYTQTQAMEKAWAPGGKTNHKDGPGEARPPPRDNSSLHSAGIPCEKELGKVRRGVEPKPEALLARRSLQPPGIESEKSEKLSSFPSLQKDGAKEPERKEQPLQRHPSSIPPPPLTAKDLSSPAARQHCSSPSHASGREPGAKPSTAEPSSSPQDPPKPVAAHSESSSHKPRPGPDPGPPKTKHPDRSLSSQKPSVGATKGKEPATQSLGGSSREGKGHSKSGPDVFPATPGSQNKASDGIGQGEGGPSVPLHTDRAPLDAKPQPTSGGRPLEVLEKPVHLPRPGHPGPSEPADQKLSAVGEKQTLSPKHPKPSTVKDCPTLCKQTDNRQTDKSPSQPAANTDRRAEGKKCTEALYAPAEGDKLEAGLSFVHSENRLKGAERPAAGVGKGFPEARGKGPGPQKPPTEADKPNGMKRSPSATGQSSFRSTALPEKSLSCSSSFPETRAGVREASAASSDTSSAKAAGGMLELPAPSNRDHRKAQPAGEGRTHMTKSDSLPSFRVSTLPLESHHPDPNTMGGASHRDRALSVTATVGETKGKDPAPAQPPPARKQNVGRDVTKPSPAPNTDRPISLSNEKDFVVRQRRGKESLRSSPHKKAL

Target ORF information:

RefSeq Version	XM_017009448.1
Organism	Homo sapiens(human)
Definition	Homo sapiens microtubule associated serine/threonine kinase family member 4 (MAST4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009448.1

ORF Insert Sequence:

ATGGGGGAGA AAGTTTCGGA GGCGCCAGAG CCGGTGCCCC GCGGCTGCAG TGGCCACGGC 
AGCCGGACTC CAGCCTCTGC GCTGGTCGCC GCGTCCTCTC CGGGTGCTTC CTCGGCCGAG 
TCCTCCTCGG GCTCAGAAAC TCTGTCGGAG GAAGGGGAGC CCGGCGGCTT CTCCAGAGAG 
CATCAGCCGC CGCCGCCGCC GCCGTTGGGA GGCACCCTGG GCGCCCGGGC GCCCGCCGCG 
TGGGCTCCGG CAAGCGTGCT GCTGGAGCGC GGAGTCCTTG CGCTGCCGCC GCCGCTTCCC 
GGAGGAGCTG TGCCGCCCGC GCCCCGGGGC AGCAGCGCGT CCCAGGAGGA GCAGGACGAG 
GAGCTTGACC ACATATTATC CCCTCCACCC ATGCCGTTTC GGAAATGCAG CAACCCAGAT 
GTGGCTTCTG GCCCTGGAAA ATCACTGAAG TATAAAAGAC AGCTGAGTGA GGATGGAAGA 
CAGCTAAGGC GAGGGAGCCT GGGAGGAGCC CTGACTGGGA GGTACCTTCT TCCAAACCCG 
GTGGCGGGAC AGGCCTGGCC GGCCTCTGCA GAGACGTCCA ACCTCGTGCG CATGCGCAGC 
CAGGCCCTGG GCCAGTCGGC GCCCTCGCTC ACCGCCAGCC TGGAAGGGCA GCCTCCGAAG 
AGCCATTTCA ACTCAGCCCG ACATCGCCAG AGACTAGTGG ACCCAGCTGC TTCAAAAAAG 
GAGCTGAGTC TCCCCAGAAG AGGAAGTTTT TGCCGAACAA GCAACCGGAA AAGCTTAATA 
GGCAATGGGC AGTCACCAGC ATTGCCTCGA CCACACTCAC CTCTCTCTGC TCATGCAGGA 
AATAGCCCTC AAGATAGTCC AAGAAATTTC TCCCCCAGTG CCTCAGCCCA TTTTTCATTT 
GCACGGAGGA CTGATGGACG CCGCTGGTCG TTGGCTTCTC TCCCTTCCTC TGGCTATGGG 
ACAAACACAC CCAGCTCTAC GGTCTCTTCA TCCTGTTCCT CCCAGGAGAA GTTGCATCAG 
TTACCATACC AACCAACACC AGACGAGTTA CACTTCTTAT CAAAACATTT CTGTACCACC 
GAAAGCATCG CCACTGAGAA CAGATGCAGG AACACGCCGA TGCGCCCCCG TTCCCGAAGT 
CTGAGCCCTG GACGTTCTCC CGCCTGCTGT GACCATGAAA TAATTATGAT GAACCATGTC 
TACAAAGAAA GGTTCCCAAA GGCTACAGCT CAGATGGAAG AACGTCTAAA GGAAATTATC 
ACCAGCTACT CTCCTGACAA CGTTCTACCC TTAGCAGATG GAGTGCTTAG TTTCACTCAC 
CACCAGATTA TTGAACTGGC TCGAGATTGC TTGGATAAAT CCCACCAGGG CCTCATCACC 
TCACGATACT TCCTTGAATT ACAGCACAAA TTAGATAAGT TGCTACAGGA GGCTCATGAT 
CGTTCAGAAA GTGGAGAATT GGCATTTATT AAACAACTAG TTCGAAAGAT CCTAATTGTT 
ATTGCCCGCC CTGCTCGGTT ATTAGAGTGC CTGGAATTTG ATCCGGAAGA ATTTTACTAC 
CTATTGGAAG CAGCAGAAGG CCATGCCAAA GAAGGACAGG GTATTAAAAC CGACATTCCC 
AGGTACATCA TTAGCCAACT GGGACTCAAT AAGGATCCCT TGGAAGAAAT GGCTCATTTG 
GGAAACTACG ATAGTGGGAC AGCAGAAACA CCAGAAACAG ATGAATCAGT GAGTAGCTCT 
AATGCCTCCC TGAAACTTCG AAGGAAACCT CGGGAAAGTG ATTTTGAAAC GATTAAATTG 
ATTAGCAATG GAGCCTATGG GGCAGTCTAC TTTGTTCGGC ATAAAGAATC CCGGCAGAGG 
TTTGCCATGA AGAAGATTAA TAAACAGAAC CTCATCCTTC GAAACCAGAT CCAGCAGGCC 
TTTGTGGAGC GGGATATCCT GACTTTTGCA GAAAACCCCT TTGTTGTCAG CATGTATTGC 
TCCTTTGAAA CAAGGCGCCA CTTGTGCATG GTCATGGAAT ATGTGGAAGG GGGAGACTGT 
GCTACTTTAA TGAAAAACAT GGGTCCTCTC CCTGTTGATA TGGCCAGAAT GTACTTTGCT 
GAGACGGTCT TGGCCTTGGA ATATTTACAT AATTATGGAA TTGTACACAG GGATTTGAAA 
CCAGACAACT TGTTGGTTAC CTCCATGGGG CACATAAAGC TGACAGATTT TGGATTATCT 
AAGGTGGGAC TAATGAGCAT GACTACCAAC CTTTACGAGG GTCATATTGA GAAGGATGCT 
AGAGAGTTCC TGGATAAACA GGTCTGTGGC ACACCTGAAT ACATTGCACC AGAAGTGATT 
CTGAGGCAGG GTTATGGAAA GCCGGTGGAC TGGTGGGCCA TGGGGATTAT CCTCTATGAA 
TTTCTGGTTG GATGCGTGCC ATTCTTTGGG GATACTCCAG AGGAGCTATT TGGACAAGTC 
ATCAGTGATG AGATCAACTG GCCTGAGAAG GATGAGGCAC CCCCACCTGA TGCCCAGGAT 
CTGATTACCT TACTCCTCAG GCAGAATCCC CTGGAGAGGC TGGGAACAGG TGGTGCATAT 
GAAGTCAAAC AGCATCGATT CTTCCGTTCT TTAGACTGGA ACAGTTTGCT GAGACAGAAG 
GCAGAATTTA TTCCCCAACT GGAATCTGAG GATGACACAA GTTATTTTGA TACTCGGTCT 
GAGAAGTATC ATCATATGGA AACGGAGGAA GAAGATGACA CAAATGATGA AGACTTTAAT 
GTGGAAATAA GGCAGTTTTC TTCATGTTCA CACAGGTTTT CAAAAGTTTT CAGCAGTATA 
GATCGAATCA CTCAGAATTC AGCAGAAGAG AAGGAAGACT CTGTGGACAA AACCAAAAGC 
ACCACCTTGC CATCCACAGA AACACTGAGC TGGAGTTCAG AATATTCTGA AATGCAACAG 
CTATCAACAT CCAACTCTTC AGATACTGAA AGCAACAGAC ATAAACTCAG TTCTGGCCTA 
CTTCCCAAAC TGGCTATTTC AACAGAGGGA GAGCAAGATG AAGCTGCCTC CTGCCCTGGA 
GACCCCCATG AGGAGCCAGG AAAGCCAGCC CTTCCTCCTG AAGAGTGTGC CCAGGAGGAG 
CCTGAGGTCA CCACCCCAGC CAGCACCATC AGCAGCTCCA CCCTGTCAGT TGGCAGTTTT 
TCAGAGCACT TGGATCAGAT AAATGGACGA AGCGAGTGTG TGGACAGTAC AGATAATTCC 
TCAAAGCCAT CCAGTGAACC CGCTTCTCAC ATGGCTCGGC AGCGATTAGA AAGCACAGAA 
AAAAAGAAAA TCTCGGGGAA AGTCACAAAG TCCCTCTCTG CCAGTGCTCT TTCCCTCATG 
ATCCCAGGAG ATATGTTTGC TGTTTCCCCT CTGGGAAGTC CAATGTCTCC CCATTCCCTG 
TCCTCGGACC CTTCTTCTTC ACGAGATTCC TCTCCCAGCC GAGATTCCTC AGCAGCTTCT 
GCCAGTCCAC ATCAGCCGAT TGTGATCCAC AGTTCGGGGA AGAACTACGG CTTTACCATC 
CGAGCCATCC GGGTGTATGT GGGAGACAGT GACATCTATA CAGTGCACCA TATCGTCTGG 
AATGTAGAAG AAGGAAGTCC GGCATGCCAG GCAGGACTGA AGGCTGGAGA TCTTATCACT 
CACATCAATG GAGAACCAGT GCATGGACTT GTCCACACAG AAGTTATAGA ACTCCTACTG 
AAGAGTGGGA ATAAGGTGTC AATCACTACT ACCCCATTTG AAAACACATC AATCAAAACT 
GGACCAGCCA GGAGAAACAG CTATAAGAGC CGGATGGTGA GGCGGAGCAA GAAATCCAAG 
AAGAAAGAAA GTCTCGAAAG GAGGAGATCT CTTTTCAAAA AGCTAGCCAA GCAGCCTTCT 
CCTTTACTCC ACACCAGCCG AAGTTTCTCC TGCTTGAACA GATCCCTGTC ATCGGGTGAG 
AGCCTCCCAG GTTCCCCCAC TCATAGCTTG TCTCCCCGGT CTCCAACACC AAGCTACCGC 
TCCACCCCTG ACTTCCCATC TGGTACTAAT TCCTCCCAGA GCAGCTCCCC TAGTTCTAGT 
GCCCCCAATT CCCCAGCAGG GTCCGGGCAC ATCCGGCCCA GCACTCTCCA CGGTCTTGCA 
CCCAAACTCG GCGGGCAGCG GTACCGGTCC GGAAGGCGAA AGTCCGCCGG CAACATCCCA 
CTGTCCCCGC TGGCCCGGAC GCCCTCTCCA ACCCCGCAAC CCACCTCCCC GCAGCGGTCA 
CCATCCCCTC TTCTGGGACA CTCACTGGGC AATTCCAAGA TCGCGCAAGC CTTTCCCAGC 
AAGATGCACT CCCCGCCCAC CATCGTCAGA CACATCGTGA GGCCCAAGAG TGCGGAGCCC 
CCCAGGTCCC CGCTGCTCAA GCGCGTGCAG TCCGAGGAGA AGCTGTCGCC CTCTTACGGC 
AGTGACAAGA AGCACCTGTG CTCCCGCAAG CACAGCCTGG AGGTGACCCA AGAGGAGGTG 
CAGCGGGAGC AGTCCCAGCG GGAGGCGCCG CTGCAGAGCC TGGATGAGAA CGTGTGCGAC 
GTGCCGCCGC TCAGCCGCGC CCGGCCAGTG GAGCAAGGCT GCCTGAAACG CCCAGTCTCC 
CGGAAGGTGG GCCGCCAGGA GTCTGTGGAC GACCTGGACC GCGACAAGCT GAAGGCCAAG 
GTGGTGGTGA AGAAAGCAGA CGGCTTCCCA GAGAAACAGG AATCCCACCA GAAATCCCAT 
GGACCCGGGA GTGATTTGGA AAACTTTGCT CTGTTTAAGC TGGAAGAGAG AGAGAAGAAA 
GTCTATCCGA AGGCTGTGGA AAGGTCAAGT ACTTTTGAAA ACAAAGCGTC TATGCAGGAG 
GCGCCACCGC TGGGCAGCCT GCTGAAGGAT GCTCTTCACA AGCAGGCCAG CGTGCGCGCC 
AGCGAGGGTG CGATGTCGGA TGGCCGGGTG CCTGCGGAGC ACCGCCAGGG TGGCGGGGAC 
TTCAGACGGG CCCCCGCTCC TGGCACCCTC CAGGATGGTC TCTGCCACTC CCTCGACAGG 
GGCATCTCTG GGAAGGGGGA AGGCACGGAG AAGTCCTCCC AGGCCAAGGA GCTTCTCCGA 
TGTGAAAAGT TAGACAGCAA GCTGGCCAAC ATCGATTACC TCCGAAAGAA AATGTCACTT 
GAGGACAAAG AGGACAACCT CTGCCCTGTG CTGAAGCCCA AGATGACAGC TGGCTCCCAC 
GAATGCCTGC CAGGGAACCC AGTCCGACCC ACGGGTGGGC AGCAGGAGCC CCCGCCGGCT 
TCTGAGAGCC GAGCTTTTGT CAGCAGCACC CATGCAGCTC AGATGAGTGC CGTCTCTTTT 
GTTCCCCTCA AGGCCTTAAC AGGCCGGGTG GACAGTGGAA CGGAGAAGCC TGGCTTGGTT 
GCTCCTGAGT CCCCTGTTAG GAAGAGCCCC TCCGAGTATA AGCTGGAAGG TAGGTCTGTC 
TCATGCCTGA AGCCGATCGA GGGCACTCTG GACATTGCTC TCCTGTCCGG ACCTCAGGCC 
TCCAAGACAG AACTGCCTTC CCCAGAGTCT GCACAGAGCC CCAGCCCAAG TGGTGACGTG 
AGGGCCTCTG TGCCACCAGT TCTCCCCAGC AGCAGTGGGA AAAAGAACGA TACCACCAGT 
GCAAGAGAGC TTTCTCCTTC CAGCTTAAAG ATGAATAAAT CCTACCTGCT GGAGCCTTGG 
TTCCTGCCCC CCAGCCGAGG TCTCCAGAAT TCACCAGCAG TTTCCCTGCC TGACCCAGAG 
TTCAAGAGGG ACAGGAAAGG TCCCCATCCT ACTGCCAGGA GCCCTGGAAC AGTCATGGAA 
AGCAATCCCC AACAGAGAGA GGGCAGCTCC CCTAAACACC AAGACCACAC CACTGACCCC 
AAGCTTCTGA CCTGCCTGGG GCAGAACCTC CACAGCCCTG ACCTGGCCAG GCCACGCTGC 
CCGCTCCCAC CTGAAGCTTC CCCCTCAAGG GAGAAGCCAG GCCTGAGGGA ATCGTCTGAA 
AGAGGCCCTC CCACAGCCAG AAGCGAGCGC TCTGCTGCGA GGGCTGACAC ATGCAGAGAG 
CCCTCCATGG AACTGTGCTT TCCAGAAACT GCGAAAACCA GTGACAACTC CAAAAATCTC 
CTCTCTGTGG GAAGGACCCA CCCAGATTTC TATACACAGA CCCAGGCCAT GGAGAAAGCA 
TGGGCGCCGG GTGGGAAAAC GAACCACAAA GATGGCCCAG GTGAGGCGAG GCCCCCGCCC 
AGAGACAACT CCTCTCTGCA CTCAGCTGGA ATTCCCTGTG AGAAGGAGCT GGGCAAGGTG 
AGGCGTGGCG TGGAACCCAA GCCCGAAGCG CTTCTTGCCA GGCGGTCTCT GCAGCCACCT 
GGAATTGAGA GTGAGAAGAG TGAAAAGCTC TCCAGTTTCC CATCTTTGCA GAAAGATGGT 
GCCAAGGAAC CTGAAAGGAA GGAGCAGCCT CTACAAAGGC ATCCCAGCAG CATCCCTCCG 
CCCCCTCTGA CGGCCAAAGA CCTGTCCAGC CCGGCTGCCA GGCAGCATTG CAGTTCCCCA 
AGCCACGCTT CTGGCAGAGA GCCGGGGGCC AAGCCCAGCA CTGCAGAGCC CAGCTCGAGC 
CCCCAGGACC CTCCCAAGCC TGTTGCTGCG CACAGTGAAA GCAGCAGCCA CAAGCCCCGG 
CCTGGCCCTG ACCCGGGCCC TCCAAAGACT AAGCACCCCG ACCGGTCCCT CTCCTCTCAG 
AAACCAAGTG TCGGGGCCAC AAAGGGCAAA GAGCCTGCCA CTCAGTCCCT CGGTGGCTCT 
AGCAGAGAGG GGAAGGGCCA CAGTAAGAGT GGGCCGGATG TGTTTCCTGC TACCCCAGGC 
TCCCAGAACA AAGCCAGCGA TGGGATTGGC CAGGGAGAAG GTGGGCCCTC TGTCCCACTG 
CACACTGACA GGGCTCCTCT AGACGCCAAG CCACAACCCA CCAGTGGTGG GCGGCCCCTG 
GAGGTGCTGG AGAAGCCTGT GCATTTGCCA AGGCCGGGAC ACCCAGGGCC TAGTGAGCCA 
GCGGACCAGA AACTGTCCGC TGTTGGTGAA AAGCAAACCC TGTCTCCAAA GCACCCCAAA 
CCATCCACTG TGAAAGATTG CCCCACCCTG TGCAAACAGA CAGACAACAG ACAGACAGAC 
AAAAGCCCGA GTCAGCCGGC CGCCAACACC GACAGAAGGG CGGAAGGGAA GAAATGCACT 
GAAGCACTTT ATGCTCCAGC AGAGGGCGAC AAGCTCGAGG CCGGCCTTTC CTTTGTGCAT 
AGCGAGAACC GGTTGAAAGG CGCGGAGCGG CCAGCCGCGG GGGTGGGGAA GGGCTTCCCT 
GAGGCCAGAG GGAAAGGGCC CGGTCCCCAG AAGCCACCGA CGGAGGCAGA CAAGCCCAAT 
GGCATGAAAC GGTCCCCCTC AGCCACTGGG CAGAGTTCTT TCCGATCCAC GGCCCTCCCG 
GAAAAGTCTC TGAGCTGCTC CTCCAGCTTC CCTGAAACCA GGGCCGGAGT TAGAGAGGCC 
TCTGCAGCCA GCAGCGACAC CTCTTCTGCC AAGGCCGCCG GGGGCATGCT GGAGCTTCCA 
GCCCCCAGCA ACAGGGACCA TAGGAAGGCT CAGCCTGCCG GGGAGGGCCG AACCCACATG 
ACAAAGAGTG ACTCCCTGCC CTCCTTCCGG GTCTCCACCC TGCCTCTGGA GTCACACCAC 
CCCGACCCAA ACACCATGGG CGGGGCCAGC CACCGGGACA GGGCTCTCTC GGTGACTGCC 
ACCGTAGGGG AAACCAAAGG GAAGGACCCT GCCCCAGCCC AGCCTCCCCC AGCTAGGAAA 
CAGAACGTGG GCAGAGACGT GACCAAGCCA TCCCCAGCCC CAAACACTGA CCGCCCCATC 
TCTCTTTCTA ATGAGAAGGA CTTTGTGGTA CGGCAGAGGC GGGGGAAAGA GAGTTTGCGT 
AGCAGCCCTC ACAAAAAGGC CTTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide association analysis of genetic generalized epilepsies implicates susceptibility loci at 1q43, 2p16.1, 2q22.3 and 17q21.32.
Human molecular genetics21(24)5359-72(2012 Dec)
,,Steffens M,Leu C,Ruppert AK,Zara F,Striano P,Robbiano A,Capovilla G,Tinuper P,Gambardella A,Bianchi A,La Neve A,Crichiutti G,de Kovel CG,Kasteleijn-Nolst Trenit? D,de Haan GJ,Lindhout D,Gaus V,Schmitz B,Janz D,Weber YG,Becker F,Lerche H,Steinhoff BJ,Kleefu?-Lie AA,Kunz WS,Surges R,Elger CE,Muhle H,von Spiczak S,Ostertag P,Helbig I,Stephani U,M?ller RS,Hjalgrim H,Dibbens LM,Bellows S,Oliver K,Mullen S,Scheffer IE,Berkovic SF,Everett KV,Gardiner MR,Marini C,Guerrini R,Lehesjoki AE,Siren A,Guipponi M,Malafosse A,Thomas P,Nabbout R,Baulac S,Leguern E,Guerrero R,Serratosa JM,Reif PS,Rosenow F,M?rzinger M,Feucht M,Zimprich F,Kapser C,Schankin CJ,Suls A,Smets K,De Jonghe P,Jordanova A,Caglayan H,Yapici Z,Yalcin DA,Baykan B,Bebek N,Ozbek U,Gieger C,Wichmann HE,Balschun T,Ellinghaus D,Franke A,Meesters C,Becker T,Wienker TF,Hempelmann A,Schulz H,R?schendorf F,Leber M,Pauck SM,Trucks H,Toliat MR,N?rnberg P,Avanzini G,Koeleman BP,Sander T

Proteomic analysis identifies dysfunction in cellular transport, energy, and protein metabolism in different brain regions of atypical frontotemporal lobar degeneration.
Journal of proteome research11(4)2533-43(2012 Apr)
Martins-de-Souza D,Guest PC,Mann DM,Roeber S,Rahmoune H,Bauder C,Kretzschmar H,Volk B,Baborie A,Bahn S

A genome-wide association study of carotid atherosclerosis in HIV-infected men.
AIDS (London, England)24(4)583-92(2010 Feb)
Shrestha S,Irvin MR,Taylor KD,Wiener HW,Pajewski NM,Haritunians T,Delaney JA,Schambelan M,Polak JF,Arnett DK,Chen YD,Grunfeld C

[Identification of a novel human MAST4 gene, a new member of the microtubule associated serine-threonine kinase family].
Molekuliarnaia biologiia40(5)808-15(2006 Sep-Oct)
Sun L,Gu S,Li X,Sun Y,Zheng D,Yu K,Ji C,Tang R,Xie Y,Mao Y

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MAST4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

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Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

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