TOPAZ1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TOPAZ1
Entrez Gene ID	375337
Full Name	testis and ovary specific PAZ domain containing 1
Synonyms	C3orf77
General protein information	Preferred Names testis and ovary specific PAZ domain containing 1 Names testis- and ovary-specific PAZ domain-containing protein 1 testis and ovary-specific PAZ domain gene 1 topaz 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p21.31
Disorder MIM:	614412

mRNA and Protein(s)

mRNA	Protein	Name
XM_017006361.1	XP_016861850.1	protein TOPAZ1 isoform X2
NM_001145030.1	NP_001138502.1	protein TOPAZ1
NM_001145030.2	NP_001138502.1	protein TOPAZ1
XM_011533694.2	XP_011531996.1	protein TOPAZ1 isoform X1
XM_017006362.1	XP_016861851.1	protein TOPAZ1 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	TOPAZ1	NP_001138502.1
Mus musculus (house mouse)	Topaz1	NP_001186665.1
Bos taurus (cattle)	TOPAZ1	NP_001239370.1
Rattus norvegicus (Norway rat)	Topaz1	XP_006244266.1
Pan troglodytes (chimpanzee)	TOPAZ1	XP_526186.2
Xenopus tropicalis (tropical clawed frog)	topaz1	NP_001186868.1
	TOPAZ1	XP_001114967.1
Canis lupus familiaris (dog)	TOPAZ1	XP_003433200.2
Gallus gallus (chicken)	TOPAZ1	XP_003640786.2

Gene Ontology
Bibliography

Related articles in PubMed

Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
Lauc G, Huffman JE, Pu?i? M, Zgaga L, Adamczyk B, Mu?ini? A, Novokmet M, Pola?ek O, Gornik O, Kri?ti? J, Keser T, Vitart V, Scheijen B, Uh HW, Molokhia M, Patrick AL, McKeigue P, Kol?i? I, Luki? IK, Swann O, van Leeuwen FN, Ruhaak LR, Houwing-Duistermaat JJ, Slagboom PE, Beekman M, de Craen AJ, Deelder AM, Zeng Q, Wang W, Hastie ND, Gyllensten U, Wilson JF, Wuhrer M, Wright AF, Rudd PM, Hayward C, Aulchenko Y, Campbell H, Rudan I
PLoS genetics9(1)e1003225(2013)

TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates.
Baillet A, Le Bouffant R, Volff JN, Luangpraseuth A, Poumerol E, Th?pot D, Pailhoux E, Livera G, Cotinot C, Mandon-P?pin B
PloS one6(11)e26950(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following TOPAZ1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOPAZ1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10521	NM_001145030.2 Latest version!	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu79748	XM_011533694.2 Latest version!	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu94161	XM_017006361.1 Latest version!	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu94162	XM_017006362.1 Latest version!	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
View Old Accession Versions >>
OHu10521	NM_001145030.1	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TOPAZ1 ( NM_001145030.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138502.1 Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.

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CloneID	OHu10521
Clone ID Related Accession (Same CDS sequence)	NM_001145030.1 , NM_001145030.2
Accession Version	NM_001145030.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	protein TOPAZ1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093476.1, AC104187.2 and BX648094.1. On or before Feb 6, 2009 this sequence version replaced XM_371632.5, XM_941032.2, XM_001724962.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END##

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGAGTC AGAGTGGATA 
ATCAATACGC CTCTGTGGCC TTGTGATAGA CTGGATGTGC TTAATCGACA TAATTTACTC 
TGTACAATTG CACATGAAAT CTTAGCCAAG AGCCTTTATA GACAGACATT TGAAGTTTTG 
CAGAATCTAC CAGGTTTTCA AAATTCCCAA GAAACTGTGG AAGTCTCACA ATATAGCCTT 
CTTTTTAATA AGCTTCTAGG TTCCTGTATA GAAAGCAGTA GTCTTGGTAT GTCATCCTCT 
GTGGCAGAAT TCATGATTTC AAAGAGCATC CCTATTGATT TTTCCTTTCT CAGAAGATTA 
ATTACTTCTT TAGGAAGGAG TCGTTTATGG CTCAAAGCCA GAGCCCACTA CAAAAGTGCT 
CTTTCCTTGG GTTGCTACCC ACCATTGGAA GGAAATTTAT ACCGAAAACT TCTTCTAATT 
CCATCTTATT TATCTGAGAT TGAAATGCTC TTAGCTATTG AAATCTTCAT GGTATCTAAT 
GCTAGTAGTA TTCAGAGTCC TGGAACTTCT ACACAGATAC TGCAGATAGT TTTGAAAAGG 
TGTGAAGACA ACCAGTCTCG GAGCAATGAT GATTATCAAG CTGCAGTAGA AAGGTTAATT 
ATGGCTGCTC GTATATCAGA TCCAAAGCTT TTCGTTAAAC ACATGACTGT CAATGTTAAT 
AAGGAACAGG TTTATAGTTT GGAACATTGT TCTGCCCTGA AATGGTTAAA AGAGAATATG 
AAGTGGGCTG GAAAGGTTTG GCTTTTCAGT AACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138502.1
CDS	169..5247
Translation	MRRPPPLGPTTASGPEGNVRNLQKRQAPGPGAAGGCGPEAGGCRENKQKRRMVARATPGRGEVESDKSVAASGAGKAARRQVEGRRGPVSPSDSSDPRGLEAAKEAELPLQTERHTKEKRKVTEASSDDPQPGLDLVRKESLTSSESFQTVECLQSLGKESIIEGIKRRIRNKKLKSLENPPLKITENEATQNIKVEFQDELYKNTPKYSCNILSPEVENNSVLKLRDCNCFPHSKGCNDENNLPYKPDGGCMHVAENFSKKENLRSLAEKSDTNSIPQLLQTEENVMGVNKLLPEESDLYQSKTNGLLSCLQHEKNKYSIEESSVGRKPRKRMKLSEKADETVTEMNFSNEYNKSELMLQENQMIADGKEAETKSPLNVLRKVSHNTVSLMDHLLSVPETVEKETSSEHHVNAVFQKTIEPLLKEETENASEPLGYESMASKEDFKSMKSFIGKSPNEYHIERRSSREDLRSASEELKLSCQRTIPMTGKRTWPYYSCARISAWCWKKASLPESSYFLRGSQESCRQVDVPKHQTNQTHLTDSKLLLQSSLTETNTESSSKEKLDSNSNCLSSVSAVEPTLMVIKEPIIKDDKKIKSEELSRRGSEVISNTTEDTQLTSETQSLTGNKKKARGNLTKLNLTATSKDGQEANNSAGKTIHRKACIAQQTFIVPDLVKILNTGRLTNFKIPLLKNKSEKRKEVNAKSSEREAYSPLELLDNLSGADVRQNRSKENVSMMMLGPQTLSIRNSVTPVQASSDSFYNKKSYSISPSFTKQGNNSKPSNHVSEPGNIVSNKEVASLTVENNAFSCDPGYVEKSPSFCCNEQETFRPVSSEVRGRKITKNFSEVGFPDILKAYEDDVLLIDVIQDDPDLFGVSNEGELSFTSEVPKISQEPNVAGEHQSTDSKYMETPVKKEPSDDLRELPVLDCGWIKPDICASNSAESEIKRDPKDVNTSLGEVANETSENETLGDFSEQIKGSDLDEKHRFTDKVITKEEKENIYEVCKSKDSRNADFMVGECQFAVPVPKPLCLLVPPLNLSGRQEDTILNTWMNDFRFLGKHSVLKLQNPETCEIFKREKNVGVFQKSLGLMIPYKYCKFHFNTLRGCERPLCKFAHVPEQGDEKVCMDVFKKYININELCLLQRAVNIFMEYYRKFPPGVYFDLQVLNDLLNSLLKHCLLKEVFQIVNLSIMVKMLPSLKILLNIFEYVATMKLRNAVPALIDIFCKLVEAGMVLDPEHFNYIVKLLYQVQASKQEITAVLEMKSRLQMRRFKKNWKCDLDSALNKLEHCKEKGDWTKLGKLYINVKMGCEKFADFQTFCACIAETLTKNYEDERPDIPFCEFAETVSKDPQNSKVDKGVLGRIGISAMYFYHKLLQWSKGRKVLEKLYELKIHFTSLKGLIGPEKLASRCQIVNVAAEIFLKSGSLDGAIWVMRESEWIINTPLWPCDRLDVLNRHNLLCTIAHEILAKSLYRQTFEVLQNLPGFQNSQETVEVSQYSLLFNKLLGSCIESSSLGMSSSVAEFMISKSIPIDFSFLRRLITSLGRSRLWLKARAHYKSALSLGCYPPLEGNLYRKLLLIPSYLSEIEMLLAIEIFMVSNASSIQSPGTSTQILQIVLKRCEDNQSRSNDDYQAAVERLIMAARISDPKLFVKHMTVNVNKEQVYSLEHCSALKWLKENMKWAGKVWLFSNH

Target ORF information:

RefSeq Version	NM_001145030.1
Organism	Homo sapiens(human)
Definition	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145030.1

ORF Insert Sequence:

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGAGTC AGAGTGGATA 
ATCAATACGC CTCTGTGGCC TTGTGATAGA CTGGATGTGC TTAATCGACA TAATTTACTC 
TGTACAATTG CACATGAAAT CTTAGCCAAG AGCCTTTATA GACAGACATT TGAAGTTTTG 
CAGAATCTAC CAGGTTTTCA AAATTCCCAA GAAACTGTGG AAGTCTCACA ATATAGCCTT 
CTTTTTAATA AGCTTCTAGG TTCCTGTATA GAAAGCAGTA GTCTTGGTAT GTCATCCTCT 
GTGGCAGAAT TCATGATTTC AAAGAGCATC CCTATTGATT TTTCCTTTCT CAGAAGATTA 
ATTACTTCTT TAGGAAGGAG TCGTTTATGG CTCAAAGCCA GAGCCCACTA CAAAAGTGCT 
CTTTCCTTGG GTTGCTACCC ACCATTGGAA GGAAATTTAT ACCGAAAACT TCTTCTAATT 
CCATCTTATT TATCTGAGAT TGAAATGCTC TTAGCTATTG AAATCTTCAT GGTATCTAAT 
GCTAGTAGTA TTCAGAGTCC TGGAACTTCT ACACAGATAC TGCAGATAGT TTTGAAAAGG 
TGTGAAGACA ACCAGTCTCG GAGCAATGAT GATTATCAAG CTGCAGTAGA AAGGTTAATT 
ATGGCTGCTC GTATATCAGA TCCAAAGCTT TTCGTTAAAC ACATGACTGT CAATGTTAAT 
AAGGAACAGG TTTATAGTTT GGAACATTGT TCTGCCCTGA AATGGTTAAA AGAGAATATG 
AAGTGGGCTG GAAAGGTTTG GCTTTTCAGT AACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPAZ1 ( NM_001145030.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001138502.1 Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.

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CloneID	OHu10521
Clone ID Related Accession (Same CDS sequence)	NM_001145030.1 , NM_001145030.2
Accession Version	NM_001145030.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Homo sapiens(human)
Product	protein TOPAZ1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AK093476.1, AC104187.2 and BX648094.1. On May 17, 2019 this sequence version replaced NM_001145030.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000309765.4/ ENSP00000310303.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGAGTC AGAGTGGATA 
ATCAATACGC CTCTGTGGCC TTGTGATAGA CTGGATGTGC TTAATCGACA TAATTTACTC 
TGTACAATTG CACATGAAAT CTTAGCCAAG AGCCTTTATA GACAGACATT TGAAGTTTTG 
CAGAATCTAC CAGGTTTTCA AAATTCCCAA GAAACTGTGG AAGTCTCACA ATATAGCCTT 
CTTTTTAATA AGCTTCTAGG TTCCTGTATA GAAAGCAGTA GTCTTGGTAT GTCATCCTCT 
GTGGCAGAAT TCATGATTTC AAAGAGCATC CCTATTGATT TTTCCTTTCT CAGAAGATTA 
ATTACTTCTT TAGGAAGGAG TCGTTTATGG CTCAAAGCCA GAGCCCACTA CAAAAGTGCT 
CTTTCCTTGG GTTGCTACCC ACCATTGGAA GGAAATTTAT ACCGAAAACT TCTTCTAATT 
CCATCTTATT TATCTGAGAT TGAAATGCTC TTAGCTATTG AAATCTTCAT GGTATCTAAT 
GCTAGTAGTA TTCAGAGTCC TGGAACTTCT ACACAGATAC TGCAGATAGT TTTGAAAAGG 
TGTGAAGACA ACCAGTCTCG GAGCAATGAT GATTATCAAG CTGCAGTAGA AAGGTTAATT 
ATGGCTGCTC GTATATCAGA TCCAAAGCTT TTCGTTAAAC ACATGACTGT CAATGTTAAT 
AAGGAACAGG TTTATAGTTT GGAACATTGT TCTGCCCTGA AATGGTTAAA AGAGAATATG 
AAGTGGGCTG GAAAGGTTTG GCTTTTCAGT AACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001138502.1
CDS	169..5247
Translation	MRRPPPLGPTTASGPEGNVRNLQKRQAPGPGAAGGCGPEAGGCRENKQKRRMVARATPGRGEVESDKSVAASGAGKAARRQVEGRRGPVSPSDSSDPRGLEAAKEAELPLQTERHTKEKRKVTEASSDDPQPGLDLVRKESLTSSESFQTVECLQSLGKESIIEGIKRRIRNKKLKSLENPPLKITENEATQNIKVEFQDELYKNTPKYSCNILSPEVENNSVLKLRDCNCFPHSKGCNDENNLPYKPDGGCMHVAENFSKKENLRSLAEKSDTNSIPQLLQTEENVMGVNKLLPEESDLYQSKTNGLLSCLQHEKNKYSIEESSVGRKPRKRMKLSEKADETVTEMNFSNEYNKSELMLQENQMIADGKEAETKSPLNVLRKVSHNTVSLMDHLLSVPETVEKETSSEHHVNAVFQKTIEPLLKEETENASEPLGYESMASKEDFKSMKSFIGKSPNEYHIERRSSREDLRSASEELKLSCQRTIPMTGKRTWPYYSCARISAWCWKKASLPESSYFLRGSQESCRQVDVPKHQTNQTHLTDSKLLLQSSLTETNTESSSKEKLDSNSNCLSSVSAVEPTLMVIKEPIIKDDKKIKSEELSRRGSEVISNTTEDTQLTSETQSLTGNKKKARGNLTKLNLTATSKDGQEANNSAGKTIHRKACIAQQTFIVPDLVKILNTGRLTNFKIPLLKNKSEKRKEVNAKSSEREAYSPLELLDNLSGADVRQNRSKENVSMMMLGPQTLSIRNSVTPVQASSDSFYNKKSYSISPSFTKQGNNSKPSNHVSEPGNIVSNKEVASLTVENNAFSCDPGYVEKSPSFCCNEQETFRPVSSEVRGRKITKNFSEVGFPDILKAYEDDVLLIDVIQDDPDLFGVSNEGELSFTSEVPKISQEPNVAGEHQSTDSKYMETPVKKEPSDDLRELPVLDCGWIKPDICASNSAESEIKRDPKDVNTSLGEVANETSENETLGDFSEQIKGSDLDEKHRFTDKVITKEEKENIYEVCKSKDSRNADFMVGECQFAVPVPKPLCLLVPPLNLSGRQEDTILNTWMNDFRFLGKHSVLKLQNPETCEIFKREKNVGVFQKSLGLMIPYKYCKFHFNTLRGCERPLCKFAHVPEQGDEKVCMDVFKKYININELCLLQRAVNIFMEYYRKFPPGVYFDLQVLNDLLNSLLKHCLLKEVFQIVNLSIMVKMLPSLKILLNIFEYVATMKLRNAVPALIDIFCKLVEAGMVLDPEHFNYIVKLLYQVQASKQEITAVLEMKSRLQMRRFKKNWKCDLDSALNKLEHCKEKGDWTKLGKLYINVKMGCEKFADFQTFCACIAETLTKNYEDERPDIPFCEFAETVSKDPQNSKVDKGVLGRIGISAMYFYHKLLQWSKGRKVLEKLYELKIHFTSLKGLIGPEKLASRCQIVNVAAEIFLKSGSLDGAIWVMRESEWIINTPLWPCDRLDVLNRHNLLCTIAHEILAKSLYRQTFEVLQNLPGFQNSQETVEVSQYSLLFNKLLGSCIESSSLGMSSSVAEFMISKSIPIDFSFLRRLITSLGRSRLWLKARAHYKSALSLGCYPPLEGNLYRKLLLIPSYLSEIEMLLAIEIFMVSNASSIQSPGTSTQILQIVLKRCEDNQSRSNDDYQAAVERLIMAARISDPKLFVKHMTVNVNKEQVYSLEHCSALKWLKENMKWAGKVWLFSNH

Target ORF information:

RefSeq Version	NM_001145030.2
Organism	Homo sapiens(human)
Definition	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001145030.2

ORF Insert Sequence:

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGAGTC AGAGTGGATA 
ATCAATACGC CTCTGTGGCC TTGTGATAGA CTGGATGTGC TTAATCGACA TAATTTACTC 
TGTACAATTG CACATGAAAT CTTAGCCAAG AGCCTTTATA GACAGACATT TGAAGTTTTG 
CAGAATCTAC CAGGTTTTCA AAATTCCCAA GAAACTGTGG AAGTCTCACA ATATAGCCTT 
CTTTTTAATA AGCTTCTAGG TTCCTGTATA GAAAGCAGTA GTCTTGGTAT GTCATCCTCT 
GTGGCAGAAT TCATGATTTC AAAGAGCATC CCTATTGATT TTTCCTTTCT CAGAAGATTA 
ATTACTTCTT TAGGAAGGAG TCGTTTATGG CTCAAAGCCA GAGCCCACTA CAAAAGTGCT 
CTTTCCTTGG GTTGCTACCC ACCATTGGAA GGAAATTTAT ACCGAAAACT TCTTCTAATT 
CCATCTTATT TATCTGAGAT TGAAATGCTC TTAGCTATTG AAATCTTCAT GGTATCTAAT 
GCTAGTAGTA TTCAGAGTCC TGGAACTTCT ACACAGATAC TGCAGATAGT TTTGAAAAGG 
TGTGAAGACA ACCAGTCTCG GAGCAATGAT GATTATCAAG CTGCAGTAGA AAGGTTAATT 
ATGGCTGCTC GTATATCAGA TCCAAAGCTT TTCGTTAAAC ACATGACTGT CAATGTTAAT 
AAGGAACAGG TTTATAGTTT GGAACATTGT TCTGCCCTGA AATGGTTAAA AGAGAATATG 
AAGTGGGCTG GAAAGGTTTG GCTTTTCAGT AACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPAZ1 ( XM_011533694.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531996.1 Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu79748
Clone ID Related Accession (Same CDS sequence)	XM_011533694.2
Accession Version	XM_011533694.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4968bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein TOPAZ1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533694.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGAGTC AGAGTGGATA 
ATCAATACGC CTCTGTGGCC TTGTGATAGA CTGGATGTGC TTAATCGACA TAATTTACTC 
TGTACAATTG CACATGAAAT CTTAGCCAAG AGCCTTTATA GACAGACATT TGAAGTTTTG 
CAGAATCTAC CAGGTTTTCA AAATTCCCAA GAAACTGTGG AAGTCTCACA ATATAGCCTT 
CTTTTTAATA AGCTTCTAGG TTCCTGTATA GAAAGCAGTA GTCTTGGTAT GTCATCCTCT 
GTGGCAGAAT TCATGATTTC AAAGAGCATC CCTATTGATT TTTCCTTTCT CAGAAGATTA 
ATTACTTCTT TAGGAAGGAG TCGTTTATGG CTCAAAGCCA GAGCCCACTA CAAAAGTGCT 
CTTTCCTTGG GTTGCTACCC ACCATTGGAA GGAAATTTAT ACCGAAAACT TCTTCTAATT 
CCATCTTATT TATCTGAGAT TGAAATGCTC TTAGCTATTG AAATCTTCAT GGTATCTAAT 
GCTAGTAGTA TTCAGAGTCC TGGAACTTCT ACACAGATAC TGCAGATAGT TTTGAAAAGC 
CACAATCAGA TTTGCCCTGG GATCATCCAC AGAGATGGAA AAGACAGGGC CGTGGATGGC 
TTCAAGGCCC CATCTAGAGG ATGGATGACT GACATTTCAG AGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531996.1
CDS	169..5136
Translation	MRRPPPLGPTTASGPEGNVRNLQKRQAPGPGAAGGCGPEAGGCRENKQKRRMVARATPGRGEVESDKSVAASGAGKAARRQVEGRRGPVSPSDSSDPRGLEAAKEAELPLQTERHTKEKRKVTEASSDDPQPGLDLVRKESLTSSESFQTVECLQSLGKESIIEGIKRRIRNKKLKSLENPPLKITENEATQNIKVEFQDELYKNTPKYSCNILSPEVENNSVLKLRDCNCFPHSKGCNDENNLPYKPDGGCMHVAENFSKKENLRSLAEKSDTNSIPQLLQTEENVMGVNKLLPEESDLYQSKTNGLLSCLQHEKNKYSIEESSVGRKPRKRMKLSEKADETVTEMNFSNEYNKSELMLQENQMIADGKEAETKSPLNVLRKVSHNTVSLMDHLLSVPETVEKETSSEHHVNAVFQKTIEPLLKEETENASEPLGYESMASKEDFKSMKSFIGKSPNEYHIERRSSREDLRSASEELKLSCQRTIPMTGKRTWPYYSCARISAWCWKKASLPESSYFLRGSQESCRQVDVPKHQTNQTHLTDSKLLLQSSLTETNTESSSKEKLDSNSNCLSSVSAVEPTLMVIKEPIIKDDKKIKSEELSRRGSEVISNTTEDTQLTSETQSLTGNKKKARGNLTKLNLTATSKDGQEANNSAGKTIHRKACIAQQTFIVPDLVKILNTGRLTNFKIPLLKNKSEKRKEVNAKSSEREAYSPLELLDNLSGADVRQNRSKENVSMMMLGPQTLSIRNSVTPVQASSDSFYNKKSYSISPSFTKQGNNSKPSNHVSEPGNIVSNKEVASLTVENNAFSCDPGYVEKSPSFCCNEQETFRPVSSEVRGRKITKNFSEVGFPDILKAYEDDVLLIDVIQDDPDLFGVSNEGELSFTSEVPKISQEPNVAGEHQSTDSKYMETPVKKEPSDDLRELPVLDCGWIKPDICASNSAESEIKRDPKDVNTSLGEVANETSENETLGDFSEQIKGSDLDEKHRFTDKVITKEEKENIYEVCKSKDSRNADFMVGECQFAVPVPKPLCLLVPPLNLSGRQEDTILNTWMNDFRFLGKHSVLKLQNPETCEIFKREKNVGVFQKSLGLMIPYKYCKFHFNTLRGCERPLCKFAHVPEQGDEKVCMDVFKKYININELCLLQRAVNIFMEYYRKFPPGVYFDLQVLNDLLNSLLKHCLLKEVFQIVNLSIMVKMLPSLKILLNIFEYVATMKLRNAVPALIDIFCKLVEAGMVLDPEHFNYIVKLLYQVQASKQEITAVLEMKSRLQMRRFKKNWKCDLDSALNKLEHCKEKGDWTKLGKLYINVKMGCEKFADFQTFCACIAETLTKNYEDERPDIPFCEFAETVSKDPQNSKVDKGVLGRIGISAMYFYHKLLQWSKGRKVLEKLYELKIHFTSLKGLIGPEKLASRCQIVNVAAEIFLKSGSLDGAIWVMRESEWIINTPLWPCDRLDVLNRHNLLCTIAHEILAKSLYRQTFEVLQNLPGFQNSQETVEVSQYSLLFNKLLGSCIESSSLGMSSSVAEFMISKSIPIDFSFLRRLITSLGRSRLWLKARAHYKSALSLGCYPPLEGNLYRKLLLIPSYLSEIEMLLAIEIFMVSNASSIQSPGTSTQILQIVLKSHNQICPGIIHRDGKDRAVDGFKAPSRGWMTDISEA

Target ORF information:

RefSeq Version	XM_011533694.2
Organism	Homo sapiens(human)
Definition	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533694.2

ORF Insert Sequence:

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGAGTC AGAGTGGATA 
ATCAATACGC CTCTGTGGCC TTGTGATAGA CTGGATGTGC TTAATCGACA TAATTTACTC 
TGTACAATTG CACATGAAAT CTTAGCCAAG AGCCTTTATA GACAGACATT TGAAGTTTTG 
CAGAATCTAC CAGGTTTTCA AAATTCCCAA GAAACTGTGG AAGTCTCACA ATATAGCCTT 
CTTTTTAATA AGCTTCTAGG TTCCTGTATA GAAAGCAGTA GTCTTGGTAT GTCATCCTCT 
GTGGCAGAAT TCATGATTTC AAAGAGCATC CCTATTGATT TTTCCTTTCT CAGAAGATTA 
ATTACTTCTT TAGGAAGGAG TCGTTTATGG CTCAAAGCCA GAGCCCACTA CAAAAGTGCT 
CTTTCCTTGG GTTGCTACCC ACCATTGGAA GGAAATTTAT ACCGAAAACT TCTTCTAATT 
CCATCTTATT TATCTGAGAT TGAAATGCTC TTAGCTATTG AAATCTTCAT GGTATCTAAT 
GCTAGTAGTA TTCAGAGTCC TGGAACTTCT ACACAGATAC TGCAGATAGT TTTGAAAAGC 
CACAATCAGA TTTGCCCTGG GATCATCCAC AGAGATGGAA AAGACAGGGC CGTGGATGGC 
TTCAAGGCCC CATCTAGAGG ATGGATGACT GACATTTCAG AGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPAZ1 ( XM_017006361.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861850.1 Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X2, mRNA.

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CloneID	OHu94161
Clone ID Related Accession (Same CDS sequence)	XM_017006361.1
Accession Version	XM_017006361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4710bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein TOPAZ1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGGTGC TCTTTCCTTG 
GGTTGCTACC CACCATTGGA AGGAAATTTA TACCGAAAAC TTCTTCTAAT TCCATCTTAT 
TTATCTGAGA TTGAAATGCT CTTAGCTATT GAAATCTTCA TGGTATCTAA TGCTAGTAGT 
ATTCAGAGTC CTGGAACTTC TACACAGATA CTGCAGATAG TTTTGAAAAG GTGTGAAGAC 
AACCAGTCTC GGAGCAATGA TGATTATCAA GCTGCAGTAG AAAGGTTAAT TATGGCTGCT 
CGTATATCAG ATCCAAAGCT TTTCGTTAAA CACATGACTG TCAATGTTAA TAAGGAACAG 
GTTTATAGTT TGGAACATTG TTCTGCCCTG AAATGGTTAA AAGAGAATAT GAAGTGGGCT 
GGAAAGGTTT GGCTTTTCAG TAACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861850.1
CDS	169..4878
Translation	MRRPPPLGPTTASGPEGNVRNLQKRQAPGPGAAGGCGPEAGGCRENKQKRRMVARATPGRGEVESDKSVAASGAGKAARRQVEGRRGPVSPSDSSDPRGLEAAKEAELPLQTERHTKEKRKVTEASSDDPQPGLDLVRKESLTSSESFQTVECLQSLGKESIIEGIKRRIRNKKLKSLENPPLKITENEATQNIKVEFQDELYKNTPKYSCNILSPEVENNSVLKLRDCNCFPHSKGCNDENNLPYKPDGGCMHVAENFSKKENLRSLAEKSDTNSIPQLLQTEENVMGVNKLLPEESDLYQSKTNGLLSCLQHEKNKYSIEESSVGRKPRKRMKLSEKADETVTEMNFSNEYNKSELMLQENQMIADGKEAETKSPLNVLRKVSHNTVSLMDHLLSVPETVEKETSSEHHVNAVFQKTIEPLLKEETENASEPLGYESMASKEDFKSMKSFIGKSPNEYHIERRSSREDLRSASEELKLSCQRTIPMTGKRTWPYYSCARISAWCWKKASLPESSYFLRGSQESCRQVDVPKHQTNQTHLTDSKLLLQSSLTETNTESSSKEKLDSNSNCLSSVSAVEPTLMVIKEPIIKDDKKIKSEELSRRGSEVISNTTEDTQLTSETQSLTGNKKKARGNLTKLNLTATSKDGQEANNSAGKTIHRKACIAQQTFIVPDLVKILNTGRLTNFKIPLLKNKSEKRKEVNAKSSEREAYSPLELLDNLSGADVRQNRSKENVSMMMLGPQTLSIRNSVTPVQASSDSFYNKKSYSISPSFTKQGNNSKPSNHVSEPGNIVSNKEVASLTVENNAFSCDPGYVEKSPSFCCNEQETFRPVSSEVRGRKITKNFSEVGFPDILKAYEDDVLLIDVIQDDPDLFGVSNEGELSFTSEVPKISQEPNVAGEHQSTDSKYMETPVKKEPSDDLRELPVLDCGWIKPDICASNSAESEIKRDPKDVNTSLGEVANETSENETLGDFSEQIKGSDLDEKHRFTDKVITKEEKENIYEVCKSKDSRNADFMVGECQFAVPVPKPLCLLVPPLNLSGRQEDTILNTWMNDFRFLGKHSVLKLQNPETCEIFKREKNVGVFQKSLGLMIPYKYCKFHFNTLRGCERPLCKFAHVPEQGDEKVCMDVFKKYININELCLLQRAVNIFMEYYRKFPPGVYFDLQVLNDLLNSLLKHCLLKEVFQIVNLSIMVKMLPSLKILLNIFEYVATMKLRNAVPALIDIFCKLVEAGMVLDPEHFNYIVKLLYQVQASKQEITAVLEMKSRLQMRRFKKNWKCDLDSALNKLEHCKEKGDWTKLGKLYINVKMGCEKFADFQTFCACIAETLTKNYEDERPDIPFCEFAETVSKDPQNSKVDKGVLGRIGISAMYFYHKLLQWSKGRKVLEKLYELKIHFTSLKGLIGPEKLASRCQIVNVAAEIFLKSGSLDGAIWVMRGALSLGCYPPLEGNLYRKLLLIPSYLSEIEMLLAIEIFMVSNASSIQSPGTSTQILQIVLKRCEDNQSRSNDDYQAAVERLIMAARISDPKLFVKHMTVNVNKEQVYSLEHCSALKWLKENMKWAGKVWLFSNH

Target ORF information:

RefSeq Version	XM_017006361.1
Organism	Homo sapiens(human)
Definition	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006361.1

ORF Insert Sequence:

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGT TAGCAAAGAT CCACAAAACA GTAAAGTAGA TAAAGGTGTA 
CTGGGAAGAA TTGGAATCAG TGCTATGTAC TTCTATCACA AGCTGTTGCA GTGGTCCAAG 
GGAAGGAAGG TCTTAGAGAA ACTATATGAA TTAAAAATAC ACTTTACAAG TTTAAAAGGA 
CTTATAGGGC CTGAGAAGTT AGCATCAAGA TGTCAAATTG TGAATGTTGC AGCAGAAATT 
TTTCTAAAAA GTGGAAGCCT AGATGGTGCC ATTTGGGTAA TGAGGGGTGC TCTTTCCTTG 
GGTTGCTACC CACCATTGGA AGGAAATTTA TACCGAAAAC TTCTTCTAAT TCCATCTTAT 
TTATCTGAGA TTGAAATGCT CTTAGCTATT GAAATCTTCA TGGTATCTAA TGCTAGTAGT 
ATTCAGAGTC CTGGAACTTC TACACAGATA CTGCAGATAG TTTTGAAAAG GTGTGAAGAC 
AACCAGTCTC GGAGCAATGA TGATTATCAA GCTGCAGTAG AAAGGTTAAT TATGGCTGCT 
CGTATATCAG ATCCAAAGCT TTTCGTTAAA CACATGACTG TCAATGTTAA TAAGGAACAG 
GTTTATAGTT TGGAACATTG TTCTGCCCTG AAATGGTTAA AAGAGAATAT GAAGTGGGCT 
GGAAAGGTTT GGCTTTTCAG TAACCATTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TOPAZ1 ( XM_017006362.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016861851.1 Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X3, mRNA.

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CloneID	OHu94162
Clone ID Related Accession (Same CDS sequence)	XM_017006362.1
Accession Version	XM_017006362.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4068bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	protein TOPAZ1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGG GAAGGAAGGT CTTAGAGAAA CTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016861851.1
CDS	169..4236
Translation	MRRPPPLGPTTASGPEGNVRNLQKRQAPGPGAAGGCGPEAGGCRENKQKRRMVARATPGRGEVESDKSVAASGAGKAARRQVEGRRGPVSPSDSSDPRGLEAAKEAELPLQTERHTKEKRKVTEASSDDPQPGLDLVRKESLTSSESFQTVECLQSLGKESIIEGIKRRIRNKKLKSLENPPLKITENEATQNIKVEFQDELYKNTPKYSCNILSPEVENNSVLKLRDCNCFPHSKGCNDENNLPYKPDGGCMHVAENFSKKENLRSLAEKSDTNSIPQLLQTEENVMGVNKLLPEESDLYQSKTNGLLSCLQHEKNKYSIEESSVGRKPRKRMKLSEKADETVTEMNFSNEYNKSELMLQENQMIADGKEAETKSPLNVLRKVSHNTVSLMDHLLSVPETVEKETSSEHHVNAVFQKTIEPLLKEETENASEPLGYESMASKEDFKSMKSFIGKSPNEYHIERRSSREDLRSASEELKLSCQRTIPMTGKRTWPYYSCARISAWCWKKASLPESSYFLRGSQESCRQVDVPKHQTNQTHLTDSKLLLQSSLTETNTESSSKEKLDSNSNCLSSVSAVEPTLMVIKEPIIKDDKKIKSEELSRRGSEVISNTTEDTQLTSETQSLTGNKKKARGNLTKLNLTATSKDGQEANNSAGKTIHRKACIAQQTFIVPDLVKILNTGRLTNFKIPLLKNKSEKRKEVNAKSSEREAYSPLELLDNLSGADVRQNRSKENVSMMMLGPQTLSIRNSVTPVQASSDSFYNKKSYSISPSFTKQGNNSKPSNHVSEPGNIVSNKEVASLTVENNAFSCDPGYVEKSPSFCCNEQETFRPVSSEVRGRKITKNFSEVGFPDILKAYEDDVLLIDVIQDDPDLFGVSNEGELSFTSEVPKISQEPNVAGEHQSTDSKYMETPVKKEPSDDLRELPVLDCGWIKPDICASNSAESEIKRDPKDVNTSLGEVANETSENETLGDFSEQIKGSDLDEKHRFTDKVITKEEKENIYEVCKSKDSRNADFMVGECQFAVPVPKPLCLLVPPLNLSGRQEDTILNTWMNDFRFLGKHSVLKLQNPETCEIFKREKNVGVFQKSLGLMIPYKYCKFHFNTLRGCERPLCKFAHVPEQGDEKVCMDVFKKYININELCLLQRAVNIFMEYYRKFPPGVYFDLQVLNDLLNSLLKHCLLKEVFQIVNLSIMVKMLPSLKILLNIFEYVATMKLRNAVPALIDIFCKLVEAGMVLDPEHFNYIVKLLYQVQASKQEITAVLEMKSRLQMRRFKKNWKCDLDSALNKLEHCKEKGDWTKLGKLYINVKMGCEKFADFQTFCACIAETLTKNYEDERPDIPFCEFAETGKEGLRETI

Target ORF information:

RefSeq Version	XM_017006362.1
Organism	Homo sapiens(human)
Definition	Homo sapiens testis and ovary specific PAZ domain containing 1 (TOPAZ1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006362.1

ORF Insert Sequence:

ATGCGACGAC CTCCACCCCT GGGCCCCACG ACGGCCTCAG GGCCTGAAGG CAATGTGCGA 
AACCTGCAGA AGCGGCAGGC GCCAGGGCCA GGCGCGGCGG GAGGCTGTGG CCCTGAGGCC 
GGGGGGTGCC GGGAAAATAA GCAAAAGAGG AGAATGGTGG CCCGGGCCAC CCCTGGGAGA 
GGCGAGGTGG AAAGTGATAA GTCGGTTGCA GCATCAGGGG CTGGAAAGGC CGCAAGGCGT 
CAGGTGGAGG GGCGCAGGGG CCCGGTGAGC CCGTCAGACT CGTCAGACCC GCGAGGCCTA 
GAAGCAGCAA AGGAGGCTGA ACTCCCCTTG CAAACGGAAA GACACACCAA GGAAAAAAGA 
AAAGTTACTG AAGCCTCAAG TGATGATCCA CAGCCAGGGC TTGACTTGGT AAGAAAGGAA 
TCATTAACCA GTTCGGAATC TTTCCAAACA GTGGAATGCT TGCAGTCTTT GGGTAAGGAA 
AGTATAATAG AAGGTATTAA AAGAAGAATT CGAAATAAAA AACTTAAAAG CTTAGAAAAC 
CCACCTCTCA AAATAACCGA AAATGAGGCT ACACAAAATA TTAAGGTTGA ATTCCAAGAT 
GAACTGTACA AGAATACTCC AAAATATTCT TGTAATATCT TGTCACCTGA AGTAGAAAAT 
AATTCCGTTT TAAAATTACG TGATTGCAAT TGTTTCCCCC ATTCCAAGGG TTGTAATGAT 
GAAAACAACC TGCCATATAA ACCTGATGGT GGATGTATGC ATGTAGCAGA AAATTTCTCA 
AAGAAAGAAA ACCTTAGGAG TCTTGCAGAG AAGAGTGACA CAAATAGTAT TCCTCAGCTC 
TTACAAACAG AAGAAAATGT AATGGGAGTA AATAAGTTAC TACCAGAAGA GAGTGATTTA 
TACCAAAGTA AAACCAATGG CTTGCTTTCC TGCCTTCAAC ATGAAAAAAA TAAATATTCA 
ATAGAGGAGA GCAGTGTTGG GCGAAAACCC AGGAAAAGGA TGAAGTTGTC TGAAAAAGCA 
GATGAAACAG TTACTGAGAT GAACTTCTCT AATGAGTATA ACAAGTCTGA GTTGATGTTG 
CAAGAAAATC AAATGATTGC TGATGGTAAA GAAGCAGAGA CTAAAAGTCC TTTAAATGTT 
TTGAGAAAAG TAAGCCATAA TACAGTCTCT TTGATGGATC ATTTATTAAG TGTCCCAGAA 
ACAGTAGAAA AAGAAACAAG TTCTGAACAT CATGTAAATG CTGTGTTTCA GAAAACCATT 
GAACCACTTT TGAAAGAAGA AACAGAGAAT GCTTCAGAGC CGTTAGGTTA TGAAAGCATG 
GCATCGAAAG AGGATTTTAA ATCGATGAAA AGCTTCATAG GGAAATCACC TAATGAGTAC 
CATATTGAAA GGAGATCTTC ACGGGAAGAC TTAAGAAGTG CATCTGAAGA ATTGAAGTTA 
AGCTGTCAGA GAACAATACC TATGACTGGT AAAAGAACTT GGCCCTATTA TTCATGTGCT 
AGAATATCTG CCTGGTGTTG GAAAAAGGCT TCCTTGCCAG AATCAAGTTA CTTTCTTCGT 
GGGTCTCAGG AAAGTTGTAG GCAAGTTGAT GTCCCTAAAC ACCAAACAAA CCAGACCCAT 
TTAACTGACT CCAAATTATT ATTACAAAGT TCCTTAACAG AAACAAACAC TGAATCTTCA 
AGTAAAGAAA AATTAGATTC TAATTCTAAT TGTTTGTCTT CAGTTTCTGC AGTAGAACCT 
ACTTTAATGG TTATAAAGGA ACCTATAATC AAGGATGATA AAAAGATAAA ATCAGAGGAA 
CTGAGCAGAA GAGGGTCAGA GGTAATTTCT AACACTACTG AAGATACTCA ATTAACCAGT 
GAGACTCAAA GCTTAACGGG AAATAAAAAA AAAGCTAGAG GAAATTTAAC GAAACTTAAT 
TTGACAGCGA CTTCCAAAGA TGGTCAGGAA GCAAATAACT CTGCAGGCAA AACTATTCAT 
CGAAAAGCAT GCATTGCTCA ACAAACATTT ATAGTTCCAG ACTTGGTTAA AATATTGAAC 
ACAGGACGGC TGACTAATTT TAAAATTCCT TTACTTAAGA ATAAATCAGA AAAAAGAAAA 
GAAGTAAATG CCAAGTCATC AGAGAGAGAA GCTTACAGTC CTCTAGAACT TCTGGACAAT 
TTATCTGGAG CAGACGTAAG ACAGAACAGG AGTAAAGAAA ATGTCTCCAT GATGATGTTA 
GGACCTCAAA CTTTGAGCAT ACGAAATAGT GTAACTCCAG TGCAAGCTAG TTCTGACTCA 
TTCTACAATA AGAAATCCTA TAGTATTTCT CCAAGCTTTA CAAAGCAAGG TAACAATAGC 
AAACCATCTA ATCACGTCTC TGAACCAGGC AATATTGTTT CTAATAAAGA AGTTGCTTCT 
CTCACAGTTG AAAATAATGC TTTTTCTTGT GATCCTGGGT ATGTAGAGAA AAGTCCTTCC 
TTCTGCTGTA ATGAACAAGA AACATTCCGA CCAGTGTCCA GTGAAGTTAG GGGTAGAAAA 
ATAACTAAGA ATTTTTCAGA GGTAGGGTTT CCAGATATTC TTAAAGCATA TGAAGATGAC 
GTCCTCTTAA TTGATGTAAT TCAAGATGAC CCAGACCTCT TTGGAGTCTC CAATGAAGGG 
GAGCTCTCAT TTACTTCTGA GGTCCCAAAG ATAAGCCAGG AGCCCAATGT TGCTGGAGAG 
CACCAATCAA CAGACTCCAA GTACATGGAA ACTCCAGTAA AAAAAGAACC AAGTGATGAC 
TTAAGAGAAC TTCCTGTACT GGACTGTGGA TGGATAAAGC CAGACATCTG TGCTTCCAAC 
TCAGCAGAAA GTGAAATAAA ACGTGATCCC AAAGATGTAA ACACTTCCTT AGGAGAAGTT 
GCTAATGAGA CCTCTGAAAA TGAAACACTG GGAGACTTCA GTGAACAAAT AAAAGGTTCA 
GACTTGGATG AAAAGCATAG ATTTACAGAC AAAGTGATTA CCAAAGAAGA AAAAGAAAAT 
ATTTATGAAG TTTGCAAAAG CAAAGATTCT AGAAATGCAG ACTTTATGGT CGGCGAATGT 
CAATTTGCAG TACCAGTCCC GAAACCTCTG TGTTTATTAG TACCTCCTTT GAATCTAAGT 
GGTCGTCAAG AAGACACAAT ACTGAATACC TGGATGAATG ATTTCAGATT TCTGGGAAAA 
CATTCTGTCC TAAAGCTGCA GAATCCTGAA ACTTGTGAAA TATTCAAGAG GGAAAAAAAT 
GTGGGGGTGT TCCAGAAGTC CCTAGGGTTG ATGATACCCT ATAAATATTG CAAATTTCAT 
TTTAATACAT TACGTGGCTG TGAGCGACCA CTGTGCAAGT TTGCTCATGT GCCTGAACAA 
GGGGATGAAA AGGTTTGCAT GGATGTGTTT AAGAAATATA TAAATATCAA TGAACTGTGT 
TTGCTACAGC GTGCAGTAAA CATATTTATG GAGTACTACA GAAAGTTTCC CCCAGGTGTA 
TACTTTGATT TACAAGTGCT AAATGACCTT CTAAATTCTT TACTCAAACA TTGTTTGTTG 
AAAGAAGTAT TTCAGATAGT GAACCTCAGC ATAATGGTTA AAATGCTGCC TTCTCTGAAA 
ATATTACTAA ACATATTTGA GTATGTGGCA ACTATGAAAT TAAGGAATGC TGTACCTGCT 
TTAATTGATA TCTTTTGCAA GCTTGTTGAA GCCGGGATGG TGCTTGACCC AGAGCACTTT 
AACTATATTG TTAAGCTTTT ATACCAAGTA CAAGCTTCCA AACAAGAAAT AACTGCAGTT 
CTGGAAATGA AATCGAGGTT ACAGATGAGA CGATTTAAAA AGAACTGGAA GTGTGATTTA 
GATTCAGCCT TGAATAAATT AGAGCATTGT AAAGAAAAAG GTGACTGGAC CAAATTGGGA 
AAATTATACA TTAACGTAAA AATGGGCTGT GAAAAATTTG CAGATTTCCA GACATTTTGT 
GCTTGCATTG CTGAAACACT CACAAAAAAC TATGAAGATG AAAGACCAGA TATTCCCTTT 
TGTGAATTTG CTGAAACAGG GAAGGAAGGT CTTAGAGAAA CTATATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loci associated with N-glycosylation of human immunoglobulin G show pleiotropy with autoimmune diseases and haematological cancers.
PLoS genetics9(1)e1003225(2013)
Lauc G,Huffman JE,Pu?i? M,Zgaga L,Adamczyk B,Mu?ini? A,Novokmet M,Pola?ek O,Gornik O,Kri?ti? J,Keser T,Vitart V,Scheijen B,Uh HW,Molokhia M,Patrick AL,McKeigue P,Kol?i? I,Luki? IK,Swann O,van Leeuwen FN,Ruhaak LR,Houwing-Duistermaat JJ,Slagboom PE,Beekman M,de Craen AJ,Deelder AM,Zeng Q,Wang W,Hastie ND,Gyllensten U,Wilson JF,Wuhrer M,Wright AF,Rudd PM,Hayward C,Aulchenko Y,Campbell H,Rudan I

TOPAZ1, a novel germ cell-specific expressed gene conserved during evolution across vertebrates.
PloS one6(11)e26950(2011)
Baillet A,Le Bouffant R,Volff JN,Luangpraseuth A,Poumerol E,Th?pot D,Pailhoux E,Livera G,Cotinot C,Mandon-P?pin B

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TOPAZ1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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