CERKL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CERKL
Entrez Gene ID	375298
Full Name	ceramide kinase like
Synonyms	RP26
General protein information	Preferred Names ceramide kinase like Names ceramide kinase-like protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2q31.3
Summary	This gene was initially identified as a locus (RP26) associated with an autosomal recessive form of retinitis pigmentosa (arRP) disease. This gene encodes a protein with ceramide kinase-like domains, however, the protein does not phosphorylate ceramide and its target substrate is currently unknown. This protein may be a negative regulator of apoptosis in photoreceptor cells. Mutations in this gene cause a form of retinitis pigmentosa characterized by autosomal recessive cone and rod dystrophy (arCRD). Alternative splicing of this gene results in multiple transcript variants encoding different isoforms and non-coding transcripts.[provided by RefSeq, May 2010].
Disorder MIM:	608381
Disorder Html:	Retinitis pigmentosa 26, 608380 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201548.4	NP_963842.1	ceramide kinase-like protein isoform 1
NM_201548.5	NP_963842.1	ceramide kinase-like protein isoform 1
NM_001030313.2	NP_001025484.1	ceramide kinase-like protein isoform 4
NM_001030312.2	NP_001025483.1	ceramide kinase-like protein isoform 3
NM_001160277.1	NP_001153749.1	ceramide kinase-like protein isoform 7
NM_001030311.2	NP_001025482.1	ceramide kinase-like protein isoform 2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	cerkl	NP_001082943.1
Oryza sativa (rice)	Os08g0152700	NP_001061006.1
Canis lupus familiaris (dog)	CERKL	XP_545552.3
Bos taurus (cattle)	CERKL	XP_002685314.1
Mus musculus (house mouse)	Cerkl	NP_001041641.1
Homo sapiens (human)	CERKL	NP_963842.1
Gallus gallus (chicken)	CERKL	XP_421973.4
Xenopus tropicalis (tropical clawed frog)	cerkl	XP_002932061.2
Pan troglodytes (chimpanzee)	CERKL	XP_003309392.1
	CERKL	XP_002799006.1

Gene Ontology
Bibliography

Related articles in PubMed

A founder mutation in CERKL is a major cause of retinal dystrophy in Finland.
Avela K, Sankila EM, Seitsonen S, Kuuluvainen L, Barton S, Gillies S, Aittom?ki K
Acta ophthalmologica(2017 Oct)

pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation.
Chen J, Liu F, Li H, Archacki S, Gao M, Liu Y, Liao S, Huang M, Wang J, Yu S, Li C, Tang Z, Liu M
Cellular signalling27(11)2314-23(2015 Nov)

Rod-Cone Dystrophy with Initially Preserved Visual Acuity Despite Early Macular Involvement Suggests Recessive CERKL Mutations.
Khan AO, Abu-Safieh L
Ophthalmic genetics36(4)369-72(2015)

CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis.
Li C, Wang L, Zhang J, Huang M, Wong F, Liu X, Liu F, Cui X, Yang G, Chen J, Liu Y, Wang J, Liao S, Gao M, Hu X, Shu X, Wang Q, Yin Z, Tang Z, Liu M
Biochimica et biophysica acta1842(7)1121-9(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

pVHL interacts with CERKL and ubiquitinates it for oxygen dependent proteasomal degradation.
Title: pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation.

The initial presenting features of CERKL-related retinopathy are distinct and unusual. Recognition of this initial presenting phenotype can facilitate earlier molecular diagnosis and genetic counseling.
Title: Rod-Cone Dystrophy with Initially Preserved Visual Acuity Despite Early Macular Involvement Suggests Recessive CERKL Mutations.

CERKL interacts with TRX2 and plays a novel key role in the regulation of the TRX2 antioxidant pathway.
Title: CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis.

An unexpected multiplicity of CERKL transcriptional start sites (four in each species) plus a high variety of alternative splicing events primarily affecting the 5' half of the gene generate >20 fully validated mRNA isoforms in human and 23 in mouse.
Title: High transcriptional complexity of the retinitis pigmentosa CERKL gene in human and mouse.

Observational study of genetic testing. (HuGE Navigator)
Title: Simultaneous mutation detection in 90 retinal disease genes in multiple patients using a custom-designed 300-kb retinal resequencing chip.

The following CERKL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CERKL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22962	NM_001030311.2 Latest version!	Homo sapiens ceramide kinase like (CERKL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$219.50-$307.30 $439.00
OHu20435	NM_001030312.2 Latest version!	Homo sapiens ceramide kinase like (CERKL), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu21669	NM_001030313.2 Latest version!	Homo sapiens ceramide kinase like (CERKL), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu22826	NM_001160277.1 Latest version!	Homo sapiens ceramide kinase like (CERKL), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu23050	NM_201548.5 Latest version!	Homo sapiens ceramide kinase like (CERKL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
View Old Accession Versions >>
OHu23050	NM_201548.4	Homo sapiens ceramide kinase like (CERKL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$265.30 $379.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CERKL ( NM_001030311.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025482.1 Homo sapiens ceramide kinase like (CERKL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22962
Clone ID Related Accession (Same CDS sequence)	NM_001030311.2
Accession Version	NM_001030311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1677bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	ceramide kinase-like protein isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293844.1, AY690329.1, AC020595.7 and BM969366.1. This sequence is a reference standard in the RefSeqGene project. On May 19, 2009 this sequence version replaced NM_001030311.1. Transcript Variant: This variant (2) represents the longest transcript and encodes the longest isoform (2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.66484.1, SRR1803616.232442.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAATAATGGA ATATGAAGGG CACGCTCTGT CACTGCTTAA GGAATGTGAA 
CTCCAGGGAT TTGATGGTGG GCATCGGAAA CCATTGTTCG CAATACATTG GAGTGTACAG 
AGATTGTTCA CTGGCATGCA AACGTTAGAA CCAAGTGTTG TCTGTGTTGG TGGAGATGGA 
TCTGCTAGCG AAGTAGCCCA TGCTTTGCTT CTGAGAGCTC AGAAGAATGC TGGGATGGAA 
ACAGACCGAA TCCTGACTCC TGTCAGAGCA CAGCTTCCAC TTGGCTTAAT ACCAGCAGGA 
TCTACCAATG TATTGGCACA TTCTCTTCAT GGAGTTCCTC ATGTGATAAC TGCAACATTG 
CACATTATAA TGGGGCATGT ACAGCTGGTC GACGTCTGCA CCTTCAGCAC CGCTGGCAAG 
CTTCTTCGCT TTGGGTTCTC AGCCATGTTT GGCTTTGGTG GAAGAACTTT GGCTCTGGCA 
GAAAAATATC GATGGATGTC CCCTAACCAA CGGAGAGATT TTGCTGTTGT TAAGGCACTG 
GCAAAACTTA AGGCAGAAGA CTGTGAAATA TCATTTTTAC CATTTAACAG CTCTGATGAT 
GTGCAAGAAA GGAGGGCACA GGGATCTCCC AAATCTGACT GTAATGATCA ATGGCAAATG 
ATCCAGGGTC AGTTCTTGAA TGTCAGCATT ATGGCAATTC CTTGCCTGTG TTCAGTGGCA 
CCTAGAGGCT TGGCACCTAA TACCAGATTA AATAATGGAA GTATGGCTCT TATAATTGCC 
CGAAACACTT CTCGGCCAGA ATTTATAAAA CACCTGAAAA GATATGCCAG TGTAAAAAAT 
CAGTTCAATT TTCCATTTGT TGAGACTTAC ACTGTTGAGG AAGTAAAAGT TCATCCAAGG 
AATAATACTG GTGGATATAA TCCAGAGGAG GAGGAGGATG AAACTGCTTC AGAAAATTGT 
TTCCCTTGGA ATGTAGATGG TGACTTAATG GAAGTTGCAT CAGAGGTCCA TATTAGATTG 
CATCCAAGAC TTATCAGTCT TTATGGAGGA AGCATGGAAG AAATGATTCC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025482.1
CDS	102..1778
Translation	MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDIWFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIKTDVTIMEYEGHALSLLKECELQGFDGGHRKPLFAIHWSVQRLFTGMQTLEPSVVCVGGDGSASEVAHALLLRAQKNAGMETDRILTPVRAQLPLGLIPAGSTNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK

Target ORF information:

RefSeq Version	NM_001030311.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ceramide kinase like (CERKL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030311.2

ORF Insert Sequence:

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAATAATGGA ATATGAAGGG CACGCTCTGT CACTGCTTAA GGAATGTGAA 
CTCCAGGGAT TTGATGGTGG GCATCGGAAA CCATTGTTCG CAATACATTG GAGTGTACAG 
AGATTGTTCA CTGGCATGCA AACGTTAGAA CCAAGTGTTG TCTGTGTTGG TGGAGATGGA 
TCTGCTAGCG AAGTAGCCCA TGCTTTGCTT CTGAGAGCTC AGAAGAATGC TGGGATGGAA 
ACAGACCGAA TCCTGACTCC TGTCAGAGCA CAGCTTCCAC TTGGCTTAAT ACCAGCAGGA 
TCTACCAATG TATTGGCACA TTCTCTTCAT GGAGTTCCTC ATGTGATAAC TGCAACATTG 
CACATTATAA TGGGGCATGT ACAGCTGGTC GACGTCTGCA CCTTCAGCAC CGCTGGCAAG 
CTTCTTCGCT TTGGGTTCTC AGCCATGTTT GGCTTTGGTG GAAGAACTTT GGCTCTGGCA 
GAAAAATATC GATGGATGTC CCCTAACCAA CGGAGAGATT TTGCTGTTGT TAAGGCACTG 
GCAAAACTTA AGGCAGAAGA CTGTGAAATA TCATTTTTAC CATTTAACAG CTCTGATGAT 
GTGCAAGAAA GGAGGGCACA GGGATCTCCC AAATCTGACT GTAATGATCA ATGGCAAATG 
ATCCAGGGTC AGTTCTTGAA TGTCAGCATT ATGGCAATTC CTTGCCTGTG TTCAGTGGCA 
CCTAGAGGCT TGGCACCTAA TACCAGATTA AATAATGGAA GTATGGCTCT TATAATTGCC 
CGAAACACTT CTCGGCCAGA ATTTATAAAA CACCTGAAAA GATATGCCAG TGTAAAAAAT 
CAGTTCAATT TTCCATTTGT TGAGACTTAC ACTGTTGAGG AAGTAAAAGT TCATCCAAGG 
AATAATACTG GTGGATATAA TCCAGAGGAG GAGGAGGATG AAACTGCTTC AGAAAATTGT 
TTCCCTTGGA ATGTAGATGG TGACTTAATG GAAGTTGCAT CAGAGGTCCA TATTAGATTG 
CATCCAAGAC TTATCAGTCT TTATGGAGGA AGCATGGAAG AAATGATTCC AAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CERKL ( NM_001030312.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025483.1 Homo sapiens ceramide kinase like (CERKL), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu20435
Clone ID Related Accession (Same CDS sequence)	NM_001030312.2
Accession Version	NM_001030312.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	ceramide kinase-like protein isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293844.1, AY690330.1, AC020595.7 and BM969366.1. On May 19, 2009 this sequence version replaced NM_001030312.1. Transcript Variant: This variant (3) lacks four alternate in-frame exons in the central coding region, compared to variant 2. The resulting isoform (3) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.283265.1, HQ426676.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGATCTACCA ATGTATTGGC ACATTCTCTT CATGGAGTTC CTCATGTGAT AACTGCAACA 
TTGCACATTA TAATGGGGCA TGTACAGCTG GTCGACGTCT GCACCTTCAG CACCGCTGGC 
AAGCTTCTTC GCTTTGGGTT CTCAGCCATG TTTGGCTTTG GTGGAAGAAC TTTGGCTCTG 
GCAGAAAAAT ATCGATGGAT GTCCCCTAAC CAACGGAGAG ATTTTGCTGT TGTTAAGGCA 
CTGGCAAAAC TTAAGGCAGA AGACTGTGAA ATATCATTTT TACCATTTAA CAGCTCTGAT 
GATGTGCAAG AAAGGAGGGC ACAGGGATCT CCCAAATCTG ACTGTAATGA TCAATGGCAA 
ATGATCCAGG GTCAGTTCTT GAATGTCAGC ATTATGGCAA TTCCTTGCCT GTGTTCAGTG 
GCACCTAGAG GCTTGGCACC TAATACCAGA TTAAATAATG GAAGTATGGC TCTTATAATT 
GCCCGAAACA CTTCTCGGCC AGAATTTATA AAACACCTGA AAAGATATGC CAGTGTAAAA 
AATCAGTTCA ATTTTCCATT TGTTGAGACT TACACTGTTG AGGAAGTAAA AGTTCATCCA 
AGGAATAATA CTGGTGGATA TAATCCAGAG GAGGAGGAGG ATGAAACTGC TTCAGAAAAT 
TGTTTCCCTT GGAATGTAGA TGGTGACTTA ATGGAAGTTG CATCAGAGGT CCATATTAGA 
TTGCATCCAA GACTTATCAG TCTTTATGGA GGAAGCATGG AAGAAATGAT TCCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025483.1
CDS	102..1361
Translation	MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDIWFRQFKKILAGSTNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK

Target ORF information:

RefSeq Version	NM_001030312.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ceramide kinase like (CERKL), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030312.2

ORF Insert Sequence:

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGATCTACCA ATGTATTGGC ACATTCTCTT CATGGAGTTC CTCATGTGAT AACTGCAACA 
TTGCACATTA TAATGGGGCA TGTACAGCTG GTCGACGTCT GCACCTTCAG CACCGCTGGC 
AAGCTTCTTC GCTTTGGGTT CTCAGCCATG TTTGGCTTTG GTGGAAGAAC TTTGGCTCTG 
GCAGAAAAAT ATCGATGGAT GTCCCCTAAC CAACGGAGAG ATTTTGCTGT TGTTAAGGCA 
CTGGCAAAAC TTAAGGCAGA AGACTGTGAA ATATCATTTT TACCATTTAA CAGCTCTGAT 
GATGTGCAAG AAAGGAGGGC ACAGGGATCT CCCAAATCTG ACTGTAATGA TCAATGGCAA 
ATGATCCAGG GTCAGTTCTT GAATGTCAGC ATTATGGCAA TTCCTTGCCT GTGTTCAGTG 
GCACCTAGAG GCTTGGCACC TAATACCAGA TTAAATAATG GAAGTATGGC TCTTATAATT 
GCCCGAAACA CTTCTCGGCC AGAATTTATA AAACACCTGA AAAGATATGC CAGTGTAAAA 
AATCAGTTCA ATTTTCCATT TGTTGAGACT TACACTGTTG AGGAAGTAAA AGTTCATCCA 
AGGAATAATA CTGGTGGATA TAATCCAGAG GAGGAGGAGG ATGAAACTGC TTCAGAAAAT 
TGTTTCCCTT GGAATGTAGA TGGTGACTTA ATGGAAGTTG CATCAGAGGT CCATATTAGA 
TTGCATCCAA GACTTATCAG TCTTTATGGA GGAAGCATGG AAGAAATGAT TCCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CERKL ( NM_001030313.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001025484.1 Homo sapiens ceramide kinase like (CERKL), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu21669
Clone ID Related Accession (Same CDS sequence)	NM_001030313.2
Accession Version	NM_001030313.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1392bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	ceramide kinase-like protein isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293844.1, AY690331.1, AC020595.7 and BM969366.1. On May 19, 2009 this sequence version replaced NM_001030313.1. Transcript Variant: This variant (4) lacks three alternate in-frame exons in the central coding region, compared to variant 2. The resulting isoform (4) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.112314.1, SRR1803615.103756.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAAGATCTAC CAATGTATTG GCACATTCTC TTCATGGAGT TCCTCATGTG 
ATAACTGCAA CATTGCACAT TATAATGGGG CATGTACAGC TGGTCGACGT CTGCACCTTC 
AGCACCGCTG GCAAGCTTCT TCGCTTTGGG TTCTCAGCCA TGTTTGGCTT TGGTGGAAGA 
ACTTTGGCTC TGGCAGAAAA ATATCGATGG ATGTCCCCTA ACCAACGGAG AGATTTTGCT 
GTTGTTAAGG CACTGGCAAA ACTTAAGGCA GAAGACTGTG AAATATCATT TTTACCATTT 
AACAGCTCTG ATGATGTGCA AGAAAGGAGG GCACAGGGAT CTCCCAAATC TGACTGTAAT 
GATCAATGGC AAATGATCCA GGGTCAGTTC TTGAATGTCA GCATTATGGC AATTCCTTGC 
CTGTGTTCAG TGGCACCTAG AGGCTTGGCA CCTAATACCA GATTAAATAA TGGAAGTATG 
GCTCTTATAA TTGCCCGAAA CACTTCTCGG CCAGAATTTA TAAAACACCT GAAAAGATAT 
GCCAGTGTAA AAAATCAGTT CAATTTTCCA TTTGTTGAGA CTTACACTGT TGAGGAAGTA 
AAAGTTCATC CAAGGAATAA TACTGGTGGA TATAATCCAG AGGAGGAGGA GGATGAAACT 
GCTTCAGAAA ATTGTTTCCC TTGGAATGTA GATGGTGACT TAATGGAAGT TGCATCAGAG 
GTCCATATTA GATTGCATCC AAGACTTATC AGTCTTTATG GAGGAAGCAT GGAAGAAATG 
ATTCCAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001025484.1
CDS	102..1493
Translation	MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDIWFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIKTDVTRSTNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK

Target ORF information:

RefSeq Version	NM_001030313.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ceramide kinase like (CERKL), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001030313.2

ORF Insert Sequence:

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAAGATCTAC CAATGTATTG GCACATTCTC TTCATGGAGT TCCTCATGTG 
ATAACTGCAA CATTGCACAT TATAATGGGG CATGTACAGC TGGTCGACGT CTGCACCTTC 
AGCACCGCTG GCAAGCTTCT TCGCTTTGGG TTCTCAGCCA TGTTTGGCTT TGGTGGAAGA 
ACTTTGGCTC TGGCAGAAAA ATATCGATGG ATGTCCCCTA ACCAACGGAG AGATTTTGCT 
GTTGTTAAGG CACTGGCAAA ACTTAAGGCA GAAGACTGTG AAATATCATT TTTACCATTT 
AACAGCTCTG ATGATGTGCA AGAAAGGAGG GCACAGGGAT CTCCCAAATC TGACTGTAAT 
GATCAATGGC AAATGATCCA GGGTCAGTTC TTGAATGTCA GCATTATGGC AATTCCTTGC 
CTGTGTTCAG TGGCACCTAG AGGCTTGGCA CCTAATACCA GATTAAATAA TGGAAGTATG 
GCTCTTATAA TTGCCCGAAA CACTTCTCGG CCAGAATTTA TAAAACACCT GAAAAGATAT 
GCCAGTGTAA AAAATCAGTT CAATTTTCCA TTTGTTGAGA CTTACACTGT TGAGGAAGTA 
AAAGTTCATC CAAGGAATAA TACTGGTGGA TATAATCCAG AGGAGGAGGA GGATGAAACT 
GCTTCAGAAA ATTGTTTCCC TTGGAATGTA GATGGTGACT TAATGGAAGT TGCATCAGAG 
GTCCATATTA GATTGCATCC AAGACTTATC AGTCTTTATG GAGGAAGCAT GGAAGAAATG 
ATTCCAAAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CERKL ( NM_001160277.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001153749.1 Homo sapiens ceramide kinase like (CERKL), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu22826
Clone ID Related Accession (Same CDS sequence)	NM_001160277.1
Accession Version	NM_001160277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1545bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	ceramide kinase-like protein isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293844.1, BC137498.1, AY690329.1, AC020595.7 and BM969366.1. Transcript Variant: This variant (7) lacks one alternate in-frame exon in the central coding region, compared to variant 2. The resulting isoform (7) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK293844.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GTAATGGAAT ATGAAGGGCA CGCTCTGTCA CTGCTTAAGG AATGTGAACT CCAGGGATTT 
GATGGTGGGC ATCGGAAACC ATTGTTCGCA ATACATTGGA GTGTACAGAG ATTGTTCACT 
GGCATGCAAA CGTTAGAACC AAGTGTTGTC TGTGTTGGTG GAGATGGATC TGCTAGCGAA 
GTAGCCCATG CTTTGCTTCT GAGAGCTCAG AAGAATGCTG GGATGGAAAC AGACCGAATC 
CTGACTCCTG TCAGAGCACA GCTTCCACTT GGCTTAATAC CAGCAGGATC TACCAATGTA 
TTGGCACATT CTCTTCATGG AGTTCCTCAT GTGATAACTG CAACATTGCA CATTATAATG 
GGGCATGTAC AGCTGGTCGA CGTCTGCACC TTCAGCACCG CTGGCAAGCT TCTTCGCTTT 
GGGTTCTCAG CCATGTTTGG CTTTGGTGGA AGAACTTTGG CTCTGGCAGA AAAATATCGA 
TGGATGTCCC CTAACCAACG GAGAGATTTT GCTGTTGTTA AGGCACTGGC AAAACTTAAG 
GCAGAAGACT GTGAAATATC ATTTTTACCA TTTAACAGCT CTGATGATGT GCAAGAAAGG 
AGGGCACAGG GATCTCCCAA ATCTGACTGT AATGATCAAT GGCAAATGAT CCAGGGTCAG 
TTCTTGAATG TCAGCATTAT GGCAATTCCT TGCCTGTGTT CAGTGGCACC TAGAGGCTTG 
GCACCTAATA CCAGATTAAA TAATGGAAGT ATGGCTCTTA TAATTGCCCG AAACACTTCT 
CGGCCAGAAT TTATAAAACA CCTGAAAAGA TATGCCAGTG TAAAAAATCA GTTCAATTTT 
CCATTTGTTG AGACTTACAC TGTTGAGGAA GTAAAAGTTC ATCCAAGGAA TAATACTGGT 
GGATATAATC CAGAGGAGGA GGAGGATGAA ACTGCTTCAG AAAATTGTTT CCCTTGGAAT 
GTAGATGGTG ACTTAATGGA AGTTGCATCA GAGGTCCATA TTAGATTGCA TCCAAGACTT 
ATCAGTCTTT ATGGAGGAAG CATGGAAGAA ATGATTCCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001153749.1
CDS	102..1646
Translation	MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDIWFRQFKKILAVMEYEGHALSLLKECELQGFDGGHRKPLFAIHWSVQRLFTGMQTLEPSVVCVGGDGSASEVAHALLLRAQKNAGMETDRILTPVRAQLPLGLIPAGSTNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK

Target ORF information:

RefSeq Version	NM_001160277.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ceramide kinase like (CERKL), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001160277.1

ORF Insert Sequence:

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GTAATGGAAT ATGAAGGGCA CGCTCTGTCA CTGCTTAAGG AATGTGAACT CCAGGGATTT 
GATGGTGGGC ATCGGAAACC ATTGTTCGCA ATACATTGGA GTGTACAGAG ATTGTTCACT 
GGCATGCAAA CGTTAGAACC AAGTGTTGTC TGTGTTGGTG GAGATGGATC TGCTAGCGAA 
GTAGCCCATG CTTTGCTTCT GAGAGCTCAG AAGAATGCTG GGATGGAAAC AGACCGAATC 
CTGACTCCTG TCAGAGCACA GCTTCCACTT GGCTTAATAC CAGCAGGATC TACCAATGTA 
TTGGCACATT CTCTTCATGG AGTTCCTCAT GTGATAACTG CAACATTGCA CATTATAATG 
GGGCATGTAC AGCTGGTCGA CGTCTGCACC TTCAGCACCG CTGGCAAGCT TCTTCGCTTT 
GGGTTCTCAG CCATGTTTGG CTTTGGTGGA AGAACTTTGG CTCTGGCAGA AAAATATCGA 
TGGATGTCCC CTAACCAACG GAGAGATTTT GCTGTTGTTA AGGCACTGGC AAAACTTAAG 
GCAGAAGACT GTGAAATATC ATTTTTACCA TTTAACAGCT CTGATGATGT GCAAGAAAGG 
AGGGCACAGG GATCTCCCAA ATCTGACTGT AATGATCAAT GGCAAATGAT CCAGGGTCAG 
TTCTTGAATG TCAGCATTAT GGCAATTCCT TGCCTGTGTT CAGTGGCACC TAGAGGCTTG 
GCACCTAATA CCAGATTAAA TAATGGAAGT ATGGCTCTTA TAATTGCCCG AAACACTTCT 
CGGCCAGAAT TTATAAAACA CCTGAAAAGA TATGCCAGTG TAAAAAATCA GTTCAATTTT 
CCATTTGTTG AGACTTACAC TGTTGAGGAA GTAAAAGTTC ATCCAAGGAA TAATACTGGT 
GGATATAATC CAGAGGAGGA GGAGGATGAA ACTGCTTCAG AAAATTGTTT CCCTTGGAAT 
GTAGATGGTG ACTTAATGGA AGTTGCATCA GAGGTCCATA TTAGATTGCA TCCAAGACTT 
ATCAGTCTTT ATGGAGGAAG CATGGAAGAA ATGATTCCAA AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CERKL ( NM_201548.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_963842.1 Homo sapiens ceramide kinase like (CERKL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23050
Clone ID Related Accession (Same CDS sequence)	NM_201548.4 , NM_201548.5
Accession Version	NM_201548.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	ceramide kinase-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293844.1, BC137498.1, AY690329.1, AC020595.7 and BM969366.1. On May 19, 2009 this sequence version replaced NM_201548.3. Transcript Variant: This variant (1) lacks one alternate in-frame exon in the central coding region, compared to variant 2. The resulting isoform (1) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.181546.1, SRR1660803.6443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAATAATGGA ATATGAAGGG CACGCTCTGT CACTGCTTAA GGAATGTGAA 
CTCCAGGGAT TTGATGGTGT TGTCTGTGTT GGTGGAGATG GATCTGCTAG CGAAGTAGCC 
CATGCTTTGC TTCTGAGAGC TCAGAAGAAT GCTGGGATGG AAACAGACCG AATCCTGACT 
CCTGTCAGAG CACAGCTTCC ACTTGGCTTA ATACCAGCAG GATCTACCAA TGTATTGGCA 
CATTCTCTTC ATGGAGTTCC TCATGTGATA ACTGCAACAT TGCACATTAT AATGGGGCAT 
GTACAGCTGG TCGACGTCTG CACCTTCAGC ACCGCTGGCA AGCTTCTTCG CTTTGGGTTC 
TCAGCCATGT TTGGCTTTGG TGGAAGAACT TTGGCTCTGG CAGAAAAATA TCGATGGATG 
TCCCCTAACC AACGGAGAGA TTTTGCTGTT GTTAAGGCAC TGGCAAAACT TAAGGCAGAA 
GACTGTGAAA TATCATTTTT ACCATTTAAC AGCTCTGATG ATGTGCAAGA AAGGAGGGCA 
CAGGGATCTC CCAAATCTGA CTGTAATGAT CAATGGCAAA TGATCCAGGG TCAGTTCTTG 
AATGTCAGCA TTATGGCAAT TCCTTGCCTG TGTTCAGTGG CACCTAGAGG CTTGGCACCT 
AATACCAGAT TAAATAATGG AAGTATGGCT CTTATAATTG CCCGAAACAC TTCTCGGCCA 
GAATTTATAA AACACCTGAA AAGATATGCC AGTGTAAAAA ATCAGTTCAA TTTTCCATTT 
GTTGAGACTT ACACTGTTGA GGAAGTAAAA GTTCATCCAA GGAATAATAC TGGTGGATAT 
AATCCAGAGG AGGAGGAGGA TGAAACTGCT TCAGAAAATT GTTTCCCTTG GAATGTAGAT 
GGTGACTTAA TGGAAGTTGC ATCAGAGGTC CATATTAGAT TGCATCCAAG ACTTATCAGT 
CTTTATGGAG GAAGCATGGA AGAAATGATT CCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963842.1
CDS	102..1700
Translation	MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDIWFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIKTDVTIMEYEGHALSLLKECELQGFDGVVCVGGDGSASEVAHALLLRAQKNAGMETDRILTPVRAQLPLGLIPAGSTNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK

Target ORF information:

RefSeq Version	NM_201548.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ceramide kinase like (CERKL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201548.4

ORF Insert Sequence:

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAATAATGGA ATATGAAGGG CACGCTCTGT CACTGCTTAA GGAATGTGAA 
CTCCAGGGAT TTGATGGTGT TGTCTGTGTT GGTGGAGATG GATCTGCTAG CGAAGTAGCC 
CATGCTTTGC TTCTGAGAGC TCAGAAGAAT GCTGGGATGG AAACAGACCG AATCCTGACT 
CCTGTCAGAG CACAGCTTCC ACTTGGCTTA ATACCAGCAG GATCTACCAA TGTATTGGCA 
CATTCTCTTC ATGGAGTTCC TCATGTGATA ACTGCAACAT TGCACATTAT AATGGGGCAT 
GTACAGCTGG TCGACGTCTG CACCTTCAGC ACCGCTGGCA AGCTTCTTCG CTTTGGGTTC 
TCAGCCATGT TTGGCTTTGG TGGAAGAACT TTGGCTCTGG CAGAAAAATA TCGATGGATG 
TCCCCTAACC AACGGAGAGA TTTTGCTGTT GTTAAGGCAC TGGCAAAACT TAAGGCAGAA 
GACTGTGAAA TATCATTTTT ACCATTTAAC AGCTCTGATG ATGTGCAAGA AAGGAGGGCA 
CAGGGATCTC CCAAATCTGA CTGTAATGAT CAATGGCAAA TGATCCAGGG TCAGTTCTTG 
AATGTCAGCA TTATGGCAAT TCCTTGCCTG TGTTCAGTGG CACCTAGAGG CTTGGCACCT 
AATACCAGAT TAAATAATGG AAGTATGGCT CTTATAATTG CCCGAAACAC TTCTCGGCCA 
GAATTTATAA AACACCTGAA AAGATATGCC AGTGTAAAAA ATCAGTTCAA TTTTCCATTT 
GTTGAGACTT ACACTGTTGA GGAAGTAAAA GTTCATCCAA GGAATAATAC TGGTGGATAT 
AATCCAGAGG AGGAGGAGGA TGAAACTGCT TCAGAAAATT GTTTCCCTTG GAATGTAGAT 
GGTGACTTAA TGGAAGTTGC ATCAGAGGTC CATATTAGAT TGCATCCAAG ACTTATCAGT 
CTTTATGGAG GAAGCATGGA AGAAATGATT CCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CERKL ( NM_201548.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_963842.1 Homo sapiens ceramide kinase like (CERKL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23050
Clone ID Related Accession (Same CDS sequence)	NM_201548.4 , NM_201548.5
Accession Version	NM_201548.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1599bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-06
Organism	Homo sapiens(human)
Product	ceramide kinase-like protein isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK293844.1, BC137498.1, AY690329.1, AC020595.7 and BM969366.1. On Nov 22, 2018 this sequence version replaced NM_201548.4. Transcript Variant: This variant (1) lacks one alternate in-frame exon in the central coding region, compared to variant 2. The resulting isoform (1) lacks an internal segment, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.181546.1, SRR1660803.6443.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000410087.8/ ENSP00000386725.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAATAATGGA ATATGAAGGG CACGCTCTGT CACTGCTTAA GGAATGTGAA 
CTCCAGGGAT TTGATGGTGT TGTCTGTGTT GGTGGAGATG GATCTGCTAG CGAAGTAGCC 
CATGCTTTGC TTCTGAGAGC TCAGAAGAAT GCTGGGATGG AAACAGACCG AATCCTGACT 
CCTGTCAGAG CACAGCTTCC ACTTGGCTTA ATACCAGCAG GATCTACCAA TGTATTGGCA 
CATTCTCTTC ATGGAGTTCC TCATGTGATA ACTGCAACAT TGCACATTAT AATGGGGCAT 
GTACAGCTGG TCGACGTCTG CACCTTCAGC ACCGCTGGCA AGCTTCTTCG CTTTGGGTTC 
TCAGCCATGT TTGGCTTTGG TGGAAGAACT TTGGCTCTGG CAGAAAAATA TCGATGGATG 
TCCCCTAACC AACGGAGAGA TTTTGCTGTT GTTAAGGCAC TGGCAAAACT TAAGGCAGAA 
GACTGTGAAA TATCATTTTT ACCATTTAAC AGCTCTGATG ATGTGCAAGA AAGGAGGGCA 
CAGGGATCTC CCAAATCTGA CTGTAATGAT CAATGGCAAA TGATCCAGGG TCAGTTCTTG 
AATGTCAGCA TTATGGCAAT TCCTTGCCTG TGTTCAGTGG CACCTAGAGG CTTGGCACCT 
AATACCAGAT TAAATAATGG AAGTATGGCT CTTATAATTG CCCGAAACAC TTCTCGGCCA 
GAATTTATAA AACACCTGAA AAGATATGCC AGTGTAAAAA ATCAGTTCAA TTTTCCATTT 
GTTGAGACTT ACACTGTTGA GGAAGTAAAA GTTCATCCAA GGAATAATAC TGGTGGATAT 
AATCCAGAGG AGGAGGAGGA TGAAACTGCT TCAGAAAATT GTTTCCCTTG GAATGTAGAT 
GGTGACTTAA TGGAAGTTGC ATCAGAGGTC CATATTAGAT TGCATCCAAG ACTTATCAGT 
CTTTATGGAG GAAGCATGGA AGAAATGATT CCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_963842.1
CDS	100..1698
Translation	MPWRRRRNRVSALEGGREEEAPPEAAAVPPALLTSPQQTEAAAERILLRGIFEIGRDSCDVVLSERALRWRPIQPERPAGDSKYDLLCKEEFIELKDIFSVKLKRRCSVKQQRSGTLLGITLFICLKKEQNKLKNSTLDLINLSEDHCDIWFRQFKKILAGFPNRPKSLKILLNPQSHKKEATQVYYEKVEPLLKLAGIKTDVTIMEYEGHALSLLKECELQGFDGVVCVGGDGSASEVAHALLLRAQKNAGMETDRILTPVRAQLPLGLIPAGSTNVLAHSLHGVPHVITATLHIIMGHVQLVDVCTFSTAGKLLRFGFSAMFGFGGRTLALAEKYRWMSPNQRRDFAVVKALAKLKAEDCEISFLPFNSSDDVQERRAQGSPKSDCNDQWQMIQGQFLNVSIMAIPCLCSVAPRGLAPNTRLNNGSMALIIARNTSRPEFIKHLKRYASVKNQFNFPFVETYTVEEVKVHPRNNTGGYNPEEEEDETASENCFPWNVDGDLMEVASEVHIRLHPRLISLYGGSMEEMIPK

Target ORF information:

RefSeq Version	NM_201548.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ceramide kinase like (CERKL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201548.5

ORF Insert Sequence:

ATGCCCTGGA GGAGGCGCAG GAACCGGGTG AGTGCCCTGG AGGGCGGCCG GGAGGAAGAG 
GCGCCCCCGG AGGCTGCCGC TGTGCCTCCG GCGCTGTTAA CGTCCCCGCA GCAGACGGAG 
GCGGCGGCCG AGCGGATTCT GCTCCGGGGC ATCTTCGAGA TCGGGAGGGA CAGTTGTGAC 
GTGGTGCTGA GCGAGCGAGC ACTGCGGTGG CGGCCCATTC AGCCCGAGCG CCCGGCGGGT 
GATTCTAAGT ATGACTTGCT ATGTAAAGAA GAATTTATTG AACTCAAAGA CATATTCTCT 
GTGAAACTGA AACGGCGTTG TTCTGTTAAA CAGCAGAGAA GTGGTACTTT ATTAGGTATC 
ACACTCTTCA TCTGCTTGAA AAAGGAACAA AATAAACTAA AGAATTCTAC ACTTGATCTT 
ATTAATTTAA GTGAAGACCA CTGTGACATA TGGTTTAGAC AGTTCAAGAA AATATTGGCA 
GGCTTTCCAA ACAGACCGAA GTCATTAAAA ATACTCCTTA ACCCCCAAAG TCACAAAAAA 
GAAGCTACCC AGGTTTATTA TGAGAAGGTT GAACCTCTGT TGAAGCTTGC AGGAATAAAA 
ACTGATGTAA CAATAATGGA ATATGAAGGG CACGCTCTGT CACTGCTTAA GGAATGTGAA 
CTCCAGGGAT TTGATGGTGT TGTCTGTGTT GGTGGAGATG GATCTGCTAG CGAAGTAGCC 
CATGCTTTGC TTCTGAGAGC TCAGAAGAAT GCTGGGATGG AAACAGACCG AATCCTGACT 
CCTGTCAGAG CACAGCTTCC ACTTGGCTTA ATACCAGCAG GATCTACCAA TGTATTGGCA 
CATTCTCTTC ATGGAGTTCC TCATGTGATA ACTGCAACAT TGCACATTAT AATGGGGCAT 
GTACAGCTGG TCGACGTCTG CACCTTCAGC ACCGCTGGCA AGCTTCTTCG CTTTGGGTTC 
TCAGCCATGT TTGGCTTTGG TGGAAGAACT TTGGCTCTGG CAGAAAAATA TCGATGGATG 
TCCCCTAACC AACGGAGAGA TTTTGCTGTT GTTAAGGCAC TGGCAAAACT TAAGGCAGAA 
GACTGTGAAA TATCATTTTT ACCATTTAAC AGCTCTGATG ATGTGCAAGA AAGGAGGGCA 
CAGGGATCTC CCAAATCTGA CTGTAATGAT CAATGGCAAA TGATCCAGGG TCAGTTCTTG 
AATGTCAGCA TTATGGCAAT TCCTTGCCTG TGTTCAGTGG CACCTAGAGG CTTGGCACCT 
AATACCAGAT TAAATAATGG AAGTATGGCT CTTATAATTG CCCGAAACAC TTCTCGGCCA 
GAATTTATAA AACACCTGAA AAGATATGCC AGTGTAAAAA ATCAGTTCAA TTTTCCATTT 
GTTGAGACTT ACACTGTTGA GGAAGTAAAA GTTCATCCAA GGAATAATAC TGGTGGATAT 
AATCCAGAGG AGGAGGAGGA TGAAACTGCT TCAGAAAATT GTTTCCCTTG GAATGTAGAT 
GGTGACTTAA TGGAAGTTGC ATCAGAGGTC CATATTAGAT TGCATCCAAG ACTTATCAGT 
CTTTATGGAG GAAGCATGGA AGAAATGATT CCAAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A founder mutation in CERKL is a major cause of retinal dystrophy in Finland.
Acta ophthalmologica(2017 Oct)
Avela K,Sankila EM,Seitsonen S,Kuuluvainen L,Barton S,Gillies S,Aittom?ki K

pVHL interacts with Ceramide kinase like (CERKL) protein and ubiquitinates it for oxygen dependent proteasomal degradation.
Cellular signalling27(11)2314-23(2015 Nov)
Chen J,Liu F,Li H,Archacki S,Gao M,Liu Y,Liao S,Huang M,Wang J,Yu S,Li C,Tang Z,Liu M

Rod-Cone Dystrophy with Initially Preserved Visual Acuity Despite Early Macular Involvement Suggests Recessive CERKL Mutations.
Ophthalmic genetics36(4)369-72(2015)
Khan AO,Abu-Safieh L

CERKL interacts with mitochondrial TRX2 and protects retinal cells from oxidative stress-induced apoptosis.
Biochimica et biophysica acta1842(7)1121-9(2014 Jul)
Li C,Wang L,Zhang J,Huang M,Wong F,Liu X,Liu F,Cui X,Yang G,Chen J,Liu Y,Wang J,Liao S,Gao M,Hu X,Shu X,Wang Q,Yin Z,Tang Z,Liu M

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オンライン注文

CERKL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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