EIF6 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EIF6
Entrez Gene ID	3692
Full Name	eukaryotic translation initiation factor 6
Synonyms	CAB,EIF3A,ITGB4BP,b(2)gcn,eIF-6,p27(BBP),p27BBP
General protein information	Preferred Names eukaryotic translation initiation factor 6 Names eukaryotic translation initiation factor 6 B4 integrin interactor eukaryotic translation initiation factor 3A p27 beta-4 integrin-binding protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q11.22
Summary	Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jun 2012].
Disorder MIM:	602912

mRNA and Protein(s)

mRNA	Protein	Name
NM_181466.2	NP_852131.1	eukaryotic translation initiation factor 6 isoform c
NM_002212.3	NP_002203.1	eukaryotic translation initiation factor 6 isoform a
NM_181466.3	NP_852131.1	eukaryotic translation initiation factor 6 isoform c
NM_001267810.1	NP_001254739.1	eukaryotic translation initiation factor 6 isoform a
NM_002212.4	NP_002203.1	eukaryotic translation initiation factor 6 isoform a
NM_181468.2	NP_852133.1	eukaryotic translation initiation factor 6 isoform a

Pathways from BioSystems

Homologies

Homo sapiens (human)	EIF6	NP_001254739.1
Mus musculus (house mouse)	Eif6	NP_034709.1
Saccharomyces cerevisiae (baker's yeast)	TIF6	NP_015341.1
Kluyveromyces lactis	KLLA0D02046g	XP_453159.1
Magnaporthe oryzae (rice blast fungus)	MGG_01671	XP_003714657.1
Pan troglodytes (chimpanzee)	EIF6	XP_003953760.1
Danio rerio (zebrafish)	eif6	NP_957238.1
Eremothecium gossypii	AGOS_ADL030C	NP_984067.1
Arabidopsis thaliana (thale cress)	emb1624	NP_191121.1
Rattus norvegicus (Norway rat)	Eif6	NP_001032429.1
Oryza sativa (rice)	Os07g0639800	NP_001060417.1
Xenopus tropicalis (tropical clawed frog)	eif6	NP_001006884.1
Canis lupus familiaris (dog)	EIF6	XP_534401.2
Bos taurus (cattle)	EIF6	NP_777255.1
Gallus gallus (chicken)	EIF6	NP_001026019.1
Drosophila melanogaster (fruit fly)	eIF6	NP_659573.1
Caenorhabditis elegans (roundworm)	eif-6	NP_493272.1
Neurospora crassa	NCU09004	XP_959032.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001380	XP_321758.5
Schizosaccharomyces pombe (fission yeast)	tif6	NP_588491.1

Gene Ontology
Bibliography

Related articles in PubMed

High levels of eukaryotic Initiation Factor 6 (eIF6) are required for immune system homeostasis and for steering the glycolytic flux of TCR-stimulated CD4
Manfrini N, Ricciardi S, Miluzio A, Fedeli M, Scagliola A, Gallo S, Brina D, Adler T, Busch DH, Gailus-Durner V, Fuchs H, Hrab? de Angelis M, Biffo S
Developmental and comparative immunology7769-76(2017 Dec)

Translational control by mTOR-independent routes: how eIF6 organizes metabolism.
Miluzio A, Ricciardi S, Manfrini N, Alfieri R, Oliveto S, Brina D, Biffo S
Biochemical Society transactions44(6)1667-1673(2016 12)

Impact of a Eukaryotic Translation Initiation Factor 3a Polymorphism on Susceptibility to Gastric Cancer.
Liu K, Lei Z, Yao H, Lei S, Zhao H
Medical principles and practice : international journal of the Kuwait University, Health Science Centre25(5)461-5(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate that eukaryotic translation initiation factor 6 (eIF6) is a central regulator of metabolism independent from mTOR protein and myc proto-oncogene protein (Myc).
Title: Translational control by mTOR-independent routes: how eIF6 organizes metabolism.

An association between eukaryotic translation initiation factor 3a (eIF3a) gene rs77382849 polymorphism and susceptibility to gastric cancer was observed in Chinese patients.
Title: Impact of a Eukaryotic Translation Initiation Factor 3a Polymorphism on Susceptibility to Gastric Cancer.

Propose that eIF6 is necessary for malignant pleural mesothelioma growth.
Title: Expression and activity of eIF6 trigger malignant pleural mesothelioma growth in vivo.

eIF6 is a node regulator of ribosomal function and predict that prioritizing its pharmacological targeting will be of benefit in cancer and Shwachman-Bodian-Diamond syndrome
Title: eIF6 anti-association activity is required for ribosome biogenesis, translational control and tumor progression.

EIF6 plays an important role in controlling cell motility and tumor metastasis.
Title: EIF6 over-expression increases the motility and invasiveness of cancer cells by modulating the expression of a critical subset of membrane-bound proteins.

The following EIF6 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EIF6 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu07571	NM_181468.2 Latest version!	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07571	NM_001267810.1 Latest version!	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07571	NM_002212.4 Latest version!	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu07569	NM_181466.3 Latest version!	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
View Old Accession Versions >>
OHu07569	NM_181466.2	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
OHu07571	NM_002212.3	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EIF6 ( NM_002212.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002203.1 Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07571
Clone ID Related Accession (Same CDS sequence)	NM_002212.3 , NM_001267810.1 , NM_002212.4 , NM_181468.2
Accession Version	NM_002212.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 6 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448068.1, BC001119.2 and BM851166.1. This sequence is a reference standard in the RefSeqGene project. On Jun 21, 2012 this sequence version replaced NM_002212.2. Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, and 6 all encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.93114.1, SRR1163658.479168.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002203.1
CDS	97..834
Translation	MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

Target ORF information:

RefSeq Version	NM_002212.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002212.3

ORF Insert Sequence:

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF6 ( NM_001267810.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001254739.1 Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 6, mRNA.

Price & Availability↑

CloneID	OHu07571
Clone ID Related Accession (Same CDS sequence)	NM_002212.3 , NM_001267810.1 , NM_002212.4 , NM_181468.2
Accession Version	NM_001267810.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 6 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN265282.1, BQ222221.1, BC001119.2 and BM851166.1. Transcript Variant: This variant (6) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 6 all encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.460695.1, BQ222221.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2145544 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001254739.1
CDS	27..764
Translation	MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

Target ORF information:

RefSeq Version	NM_001267810.1
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001267810.1

ORF Insert Sequence:

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF6 ( NM_002212.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002203.1 Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu07571
Clone ID Related Accession (Same CDS sequence)	NM_002212.3 , NM_001267810.1 , NM_002212.4 , NM_181468.2
Accession Version	NM_002212.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 6 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC001119.2 and BM851166.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002212.3. Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, and 6 all encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189652.153922.1, SRR5189658.109685.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000374450.8/ ENSP00000363574.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002203.1
CDS	53..790
Translation	MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

Target ORF information:

RefSeq Version	NM_002212.4
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002212.4

ORF Insert Sequence:

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF6 ( NM_181468.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_852133.1 Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu07571
Clone ID Related Accession (Same CDS sequence)	NM_002212.3 , NM_001267810.1 , NM_002212.4 , NM_181468.2
Accession Version	NM_181468.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 738bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 6 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN265282.1, AF022229.1, BC001119.2 and BM851166.1. On Jun 21, 2012 this sequence version replaced NM_181468.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, and 6 all encode isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.683541.1, SRR1163658.525427.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852133.1
CDS	231..968
Translation	MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYSVFEGELSDTIPVVHASIAGCRIIGRMCVGNRHGLLVPNNTTDQELQHIRNSLPDTVQIRRVEERLSALGNVTTCNDYVALVHPDLDRETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

Target ORF information:

RefSeq Version	NM_181468.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181468.2

ORF Insert Sequence:

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGTGT GTTCGAGGGC 
GAGCTCTCCG ATACCATCCC CGTGGTGCAC GCGTCTATCG CCGGCTGCCG CATCATCGGG 
CGCATGTGTG TGGGGAACAG GCACGGTCTC CTGGTACCCA ACAATACCAC CGACCAGGAG 
CTGCAACACA TTCGCAACAG CCTCCCAGAC ACAGTGCAGA TTAGGCGGGT GGAGGAGCGG 
CTCTCAGCCT TGGGCAATGT CACCACCTGC AATGACTACG TGGCCTTGGT CCACCCAGAC 
TTGGACAGGG AGACAGAAGA AATTCTGGCA GATGTGCTCA AGGTGGAAGT CTTCAGACAG 
ACAGTGGCCG ACCAGGTGCT AGTAGGAAGC TACTGTGTCT TCAGCAATCA GGGAGGGCTG 
GTGCATCCCA AGACTTCAAT TGAAGACCAG GATGAGCTGT CCTCTCTTCT TCAAGTCCCC 
CTTGTGGCGG GGACTGTGAA CCGAGGCAGT GAGGTGATTG CTGCTGGGAT GGTGGTGAAT 
GACTGGTGTG CCTTCTGTGG CCTGGACACA ACCAGCACAG AGCTGTCAGT GGTGGAGAGT 
GTCTTCAAGC TGAATGAAGC CCAGCCTAGC ACCATTGCCA CCAGCATGCG GGATTCCCTC 
ATTGACAGCC TCACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF6 ( NM_181466.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_852131.1 Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 4, mRNA.

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CloneID	OHu07569
Clone ID Related Accession (Same CDS sequence)	NM_181466.2 , NM_181466.3
Accession Version	NM_181466.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-07-14
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 6 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448068.1, BM794263.1, BC001119.2 and BM851166.1. On Jun 21, 2012 this sequence version replaced NM_181466.1. Transcript Variant: This variant (4) contains an alternate segment compared to variant 1, which causes a frameshift, and lacks an alternate exon, which corrects the frameshift, compared to variant 1. The resulting isoform (c) lacks an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1163658.19849.1, SRR1163658.17327.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGGTG CGGCGGGAGC 
CCCGGGGCTT ACGGTGGCGG GGAGGCGTGC GCAGGAGTCA AGAGCTCCGG CAGTGGGAGG 
GTGCCGGCCC CTCTGCCCCG CCACCACCGA GTCCATGTCC CTACAGTGTG TTCGAGGGCG 
AGCTCTCCGA TACCATCCCC GTGGTGCACG CGTCTATCGC CGGCTGCCGC ATCATCGGGC 
GCATGTGTGT GGGAGACAGA AGAAATTCTG GCAGATGTGC TCAAGGTGGA AGTCTTCAGA 
CAGACAGTGG CCGACCAGGT GCTAGTAGGA AGCTACTGTG TCTTCAGCAA TCAGGGAGGG 
CTGGTGCATC CCAAGACTTC AATTGAAGAC CAGGATGAGC TGTCCTCTCT TCTTCAAGTC 
CCCCTTGTGG CGGGGACTGT GAACCGAGGC AGTGAGGTGA TTGCTGCTGG GATGGTGGTG 
AATGACTGGT GTGCCTTCTG TGGCCTGGAC ACAACCAGCA CAGAGCTGTC AGTGGTGGAG 
AGTGTCTTCA AGCTGAATGA AGCCCAGCCT AGCACCATTG CCACCAGCAT GCGGGATTCC 
CTCATTGACA GCCTCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852131.1
CDS	97..777
Translation	MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYRCGGSPGAYGGGEACAGVKSSGSGRVPAPLPRHHRVHVPTVCSRASSPIPSPWCTRLSPAAASSGACVWETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

Target ORF information:

RefSeq Version	NM_181466.2
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181466.2

ORF Insert Sequence:

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGGTG CGGCGGGAGC 
CCCGGGGCTT ACGGTGGCGG GGAGGCGTGC GCAGGAGTCA AGAGCTCCGG CAGTGGGAGG 
GTGCCGGCCC CTCTGCCCCG CCACCACCGA GTCCATGTCC CTACAGTGTG TTCGAGGGCG 
AGCTCTCCGA TACCATCCCC GTGGTGCACG CGTCTATCGC CGGCTGCCGC ATCATCGGGC 
GCATGTGTGT GGGAGACAGA AGAAATTCTG GCAGATGTGC TCAAGGTGGA AGTCTTCAGA 
CAGACAGTGG CCGACCAGGT GCTAGTAGGA AGCTACTGTG TCTTCAGCAA TCAGGGAGGG 
CTGGTGCATC CCAAGACTTC AATTGAAGAC CAGGATGAGC TGTCCTCTCT TCTTCAAGTC 
CCCCTTGTGG CGGGGACTGT GAACCGAGGC AGTGAGGTGA TTGCTGCTGG GATGGTGGTG 
AATGACTGGT GTGCCTTCTG TGGCCTGGAC ACAACCAGCA CAGAGCTGTC AGTGGTGGAG 
AGTGTCTTCA AGCTGAATGA AGCCCAGCCT AGCACCATTG CCACCAGCAT GCGGGATTCC 
CTCATTGACA GCCTCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EIF6 ( NM_181466.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_852131.1 Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu07569
Clone ID Related Accession (Same CDS sequence)	NM_181466.2 , NM_181466.3
Accession Version	NM_181466.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 681bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	eukaryotic translation initiation factor 6 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB448068.1, BM794263.1, BC001119.2 and BM851166.1. On May 31, 2019 this sequence version replaced NM_181466.2. Transcript Variant: This variant (4) contains an alternate segment compared to variant 1, which causes a frameshift, and lacks an alternate exon, which corrects the frameshift, compared to variant 1. The resulting isoform (c) lacks an internal segment compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.663007.1, SRR7410570.663031.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGGTG CGGCGGGAGC 
CCCGGGGCTT ACGGTGGCGG GGAGGCGTGC GCAGGAGTCA AGAGCTCCGG CAGTGGGAGG 
GTGCCGGCCC CTCTGCCCCG CCACCACCGA GTCCATGTCC CTACAGTGTG TTCGAGGGCG 
AGCTCTCCGA TACCATCCCC GTGGTGCACG CGTCTATCGC CGGCTGCCGC ATCATCGGGC 
GCATGTGTGT GGGAGACAGA AGAAATTCTG GCAGATGTGC TCAAGGTGGA AGTCTTCAGA 
CAGACAGTGG CCGACCAGGT GCTAGTAGGA AGCTACTGTG TCTTCAGCAA TCAGGGAGGG 
CTGGTGCATC CCAAGACTTC AATTGAAGAC CAGGATGAGC TGTCCTCTCT TCTTCAAGTC 
CCCCTTGTGG CGGGGACTGT GAACCGAGGC AGTGAGGTGA TTGCTGCTGG GATGGTGGTG 
AATGACTGGT GTGCCTTCTG TGGCCTGGAC ACAACCAGCA CAGAGCTGTC AGTGGTGGAG 
AGTGTCTTCA AGCTGAATGA AGCCCAGCCT AGCACCATTG CCACCAGCAT GCGGGATTCC 
CTCATTGACA GCCTCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852131.1
CDS	53..733
Translation	MAVRASFENNCEIGCFAKLTNTYCLVAIGGSENFYRCGGSPGAYGGGEACAGVKSSGSGRVPAPLPRHHRVHVPTVCSRASSPIPSPWCTRLSPAAASSGACVWETEEILADVLKVEVFRQTVADQVLVGSYCVFSNQGGLVHPKTSIEDQDELSSLLQVPLVAGTVNRGSEVIAAGMVVNDWCAFCGLDTTSTELSVVESVFKLNEAQPSTIATSMRDSLIDSLT

Target ORF information:

RefSeq Version	NM_181466.3
Organism	Homo sapiens(human)
Definition	Homo sapiens eukaryotic translation initiation factor 6 (EIF6), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181466.3

ORF Insert Sequence:

ATGGCGGTCC GAGCTTCGTT CGAGAACAAC TGTGAGATCG GCTGCTTTGC CAAGCTCACC 
AACACCTACT GTCTGGTAGC GATCGGAGGC TCAGAGAACT TCTACAGGTG CGGCGGGAGC 
CCCGGGGCTT ACGGTGGCGG GGAGGCGTGC GCAGGAGTCA AGAGCTCCGG CAGTGGGAGG 
GTGCCGGCCC CTCTGCCCCG CCACCACCGA GTCCATGTCC CTACAGTGTG TTCGAGGGCG 
AGCTCTCCGA TACCATCCCC GTGGTGCACG CGTCTATCGC CGGCTGCCGC ATCATCGGGC 
GCATGTGTGT GGGAGACAGA AGAAATTCTG GCAGATGTGC TCAAGGTGGA AGTCTTCAGA 
CAGACAGTGG CCGACCAGGT GCTAGTAGGA AGCTACTGTG TCTTCAGCAA TCAGGGAGGG 
CTGGTGCATC CCAAGACTTC AATTGAAGAC CAGGATGAGC TGTCCTCTCT TCTTCAAGTC 
CCCCTTGTGG CGGGGACTGT GAACCGAGGC AGTGAGGTGA TTGCTGCTGG GATGGTGGTG 
AATGACTGGT GTGCCTTCTG TGGCCTGGAC ACAACCAGCA CAGAGCTGTC AGTGGTGGAG 
AGTGTCTTCA AGCTGAATGA AGCCCAGCCT AGCACCATTG CCACCAGCAT GCGGGATTCC 
CTCATTGACA GCCTCACCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High levels of eukaryotic Initiation Factor 6 (eIF6) are required for immune system homeostasis and for steering the glycolytic flux of TCR-stimulated CD4
Developmental and comparative immunology7769-76(2017 Dec)
Manfrini N,Ricciardi S,Miluzio A,Fedeli M,Scagliola A,Gallo S,Brina D,Adler T,Busch DH,Gailus-Durner V,Fuchs H,Hrab? de Angelis M,Biffo S

Translational control by mTOR-independent routes: how eIF6 organizes metabolism.
Biochemical Society transactions44(6)1667-1673(2016 12)
Miluzio A,Ricciardi S,Manfrini N,Alfieri R,Oliveto S,Brina D,Biffo S

Impact of a Eukaryotic Translation Initiation Factor 3a Polymorphism on Susceptibility to Gastric Cancer.
Medical principles and practice : international journal of the Kuwait University, Health Science Centre25(5)461-5(2016)
Liu K,Lei Z,Yao H,Lei S,Zhao H

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EIF6 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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