ITGAX cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITGAX
Entrez Gene ID	3687
Full Name	integrin subunit alpha X
Synonyms	CD11C,SLEB6
General protein information	Preferred Names integrin subunit alpha X Names integrin alpha-X CD11 antigen-like family member C complement component 3 receptor 4 subunit integrin alpha X integrin, alpha X (antigen CD11C (p150), alpha polypeptide) integrin, alpha X (complement component 3 receptor 4 subunit) leu M5, alpha subunit leukocyte adhesion glycoprotein p150,95 alpha chain leukocyte adhesion receptor p150,95 leukocyte surface antigen p150,95, alpha subunit myeloid membrane antigen, alpha subunit p150 95 integrin alpha chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16p11.2
Summary	This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013].
Disorder MIM:	151510

mRNA and Protein(s)

mRNA	Protein	Name
NM_000887.5	NP_000878.2	integrin alpha-X isoform 2 precursor
NM_001286375.2	NP_001273304.1	integrin alpha-X isoform 1 precursor
XM_024450263.1	XP_024306031.1	integrin alpha-X isoform X1
NM_000887.4	NP_000878.2	integrin alpha-X isoform 2 precursor
NM_001286375.1	NP_001273304.1	integrin alpha-X isoform 1 precursor
XM_011545852.1	XP_011544154.1	integrin alpha-X isoform X2
XM_011545854.1	XP_011544156.1	integrin alpha-X isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ITGAX	XP_510951.3
Homo sapiens (human)	ITGAX	NP_001273304.1
Canis lupus familiaris (dog)	ITGAX	XP_547049.4
Rattus norvegicus (Norway rat)	Itgax	XP_574569.3
Mus musculus (house mouse)	Itgax	NP_067309.1

Gene Ontology
Bibliography

Related articles in PubMed

CD11c+ T-bet+ memory B cells: Immune maintenance during chronic infection and inflammation?
Winslow GM, Papillion AM, Kenderes KJ, Levack RC
Cellular immunology3218-17(2017 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Studies indicate enriched expression of TBX21 (T-bet), which is important for B cell survival and response to antigen, was observed in CD11c+ B cells.
Title: Role of CD11c

Studies indicate that CD11c+ T-bet+ memory B cells exhibit a distinct transcriptome.
Title: CD11c+ T-bet+ memory B cells: Immune maintenance during chronic infection and inflammation?

CD11c, expressed not only in Hairy cell leukemia but also in dendritic cells, macrophages and monocytes is a differentiation marker for inflammation. Prolonged inflammation in the microenvironment of CLL cells may cause a susceptibility to autoimmune disorders and secondary tumors in CLL, in this way, an increase in mortality.
Title: CD11c expression in chronic lymphocytic leukemia revisited, related with complications and survival.

CD8+ T cells of Behcet's disease patients exhibited increased CD11c expression levels, which may contribute to disease pathogenesis.
Title: CD11c is upregulated in CD8+ T cells of patients with Beh?et's disease.

Circulating CD11c+ T cells were associated with the expression of multiple adhesion molecules in women, suggesting that these cells have high tissue homing potential. These data suggest that CD11c expression distinguishes a population of circulating T cells during bacterial infection with innate capacity and mucosal homing potential.
Title: Expression of CD11c Is Associated with Unconventional Activated T Cell Subsets with High Migratory Potential.

The following ITGAX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITGAX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu58510	XM_011545854.1 Latest version!	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
OHu58508	XM_011545852.1 Latest version!	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu104138	XM_024450263.1 Latest version!	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
OHu12065	NM_001286375.2 Latest version!	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu20975	NM_000887.5 Latest version!	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
View Old Accession Versions >>
OHu20975	NM_000887.4	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$825.30 $1179.00
OHu12065	NM_001286375.1	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITGAX ( XM_011545854.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544156.1 Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu58510
Clone ID Related Accession (Same CDS sequence)	XM_011545854.1
Accession Version	XM_011545854.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	integrin alpha-X isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544156.1
CDS	89..1933
Translation	MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLR

Target ORF information:

RefSeq Version	XM_011545854.1
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545854.1

ORF Insert Sequence:

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGAX ( XM_011545852.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011544154.1 Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu58508
Clone ID Related Accession (Same CDS sequence)	XM_011545852.1
Accession Version	XM_011545852.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	integrin alpha-X isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGAAAC AAGGGCAGCT GCGTTCCCTG CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011544154.1
CDS	89..2485
Translation	MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGNKGSCVPCT

Target ORF information:

RefSeq Version	XM_011545852.1
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011545852.1

ORF Insert Sequence:

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGAAAC AAGGGCAGCT GCGTTCCCTG CACCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGAX ( XM_024450263.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024306031.1 Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X2, mRNA.

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CloneID	OHu104138
Clone ID Related Accession (Same CDS sequence)	XM_024450263.1
Accession Version	XM_024450263.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	integrin alpha-X isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGG AGGGCTTCAG CGCTGTGTTC ACACCTGATG GCCCCGTTCT GGGGGCTGTG 
GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC CTGTACCCCC CAAATATGAG CCCTACCTTC 
ATCAACATGT CTCAGGAGAA TGTGGACATG AGGGACTCTT ACCTGGGTTA CTCCACCGAG 
CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG GTCCTGGGGG CCCCCCGCTA CCAGCACACC 
GGGAAGGCTG TCATCTTCAC CCAGGTGTCC AGGCAATGGA GGATGAAGGC CGAAGTCACG 
GGGACTCAGA TCGGCTCCTA CTTCGGGGCC TCCCTCTGCT CCGTGGACGT AGACAGCGAC 
GGCAGCACCG ACCTGGTCCT CATCGGGGCC CCCCATTACT ACGAGCAGAC CCGAGGGGGC 
CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC 
TACGGGGAGC AGGGCCACCC CTGGGGTCGC TTTGGGGCGG CTCTGACAGT GCTGGGGGAT 
GTGAATGGGG ACAAGCTGAC AGACGTGGTC ATCGGGGCCC CAGGAGAGGA GGAGAACCGG 
GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG GGACCCAGCA TCAGCCCCTC CCACAGCCAG 
CGGATCGCGG GCTCCCAGCT CTCCTCCAGG CTGCAGTATT TTGGGCAGGC ACTGAGCGGG 
GGTCAAGACC TCACCCAGGA TGGACTGGTG GACCTGGCTG TGGGGGCCCG GGGCCAGGTG 
CTCCTGCTCA GGACCAGACC TGTGCTCTGG GTGGGGGTGA GCATGCAGTT CATACCTGCC 
GAGATCCCCA GGTCTGCGTT TGAGTGTCGG GAGCAGGTGG TCTCTGAGCA GACCCTGGTA 
CAGTCCAACA TCTGCCTTTA CATTGACAAA CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC 
CTCCAAAGCT CTGTGACCTT GGACCTGGCC CTCGACCCTG GCCGCCTGAG TCCCCGTGCC 
ACCTTCCAGG AAACAAAGAA CCGGAGTCTG AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA 
CACTGTGAAA ACTTCAACCT GCTGCTCCCG AGCTGCGTGG AGGACTCTGT GACCCCCATT 
ACCTTGCGTC TGAACTTCAC GCTGGTGGGC AAGCCCCTCC TTGCCTTCAG AAACCTGCGG 
CCTATGCTGG CCGCCGATGC TCAGAGATAC TTCACGGCCT CCCTACCCTT TGAGAAGAAC 
TGTGGAGCCG ACCATATCTG CCAGGACAAT CTCGGCATCT CCTTCAGCTT CCCAGGCTTG 
AAGTCCCTGC TGGTGGGGAG TAACCTGGAG CTGAACGCAG AAGTGATGGT GTGGAATGAC 
GGGGAAGACT CCTACGGAAC CACCATCACC TTCTCCCACC CCGCAGGACT GTCCTACCGC 
TACGTGGCAG AGGGCCAGAA ACAAGGGCAG CTGCGTTCCC TGCACCTGAC ATGTGACAGC 
GCCCCAGTTG GGAGCCAGGG CACCTGGAGC ACCAGCTGCA GAATCAACCA CCTCATCTTC 
CGTGGCGGCG CCCAGATCAC CTTCTTGGCT ACCTTTGACG TCTCCCCCAA GGCTGTCCTG 
GGAGACCGGC TGCTTCTGAC AGCCAATGTG AGCAGTGAGA ACAACACTCC CAGGACCAGC 
AAGACCACCT TCCAGCTGGA GCTCCCGGTG AAGTATGCTG TCTACACTGT GGTTAGCAGC 
CACGAACAAT TCACCAAATA CCTCAACTTC TCAGAGTCTG AGGAGAAGGA AAGCCATGTG 
GCCATGCACA GATACCAGGT CAATAACCTG GGACAGAGGG ACCTGCCTGT CAGCATCAAC 
TTCTGGGTGC CTGTGGAGCT GAACCAGGAG GCTGTGTGGA TGGATGTGGA GGTCTCCCAC 
CCCCAGAACC CATCCCTTCG GTGCTCCTCA GAGAAAATCG CACCCCCAGC ATCTGACTTC 
CTGGCGCACA TTCAGAAGAA TCCCGTGCTG GACTGCTCCA TTGCTGGCTG CCTGCGGTTC 
CGCTGTGACG TCCCCTCCTT CAGCGTCCAG GAGGAGCTGG ATTTCACCCT GAAGGGCAAC 
CTCAGCTTTG GCTGGGTCCG CCAGATATTG CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT 
GAAATTACGT TCGACACATC CGTGTACTCC CAGCTTCCAG GACAGGAGGC ATTTATGAGA 
GCTCAGACGA CAACGGTGCT GGAGAAGTAC AAGGTCCACA ACCCCACCCC CCTCATCGTA 
GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG GCACTCATCA CAGCGGTACT GTACAAAGTT 
GGCTTCTTCA AGCGTCAGTA CAAGGAAATG ATGGAGGAGG CAAATGGACA AATTGCCCCA 
GAAAACGGGA CACAGACCCC CAGCCCGCCC AGTGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024306031.1
CDS	495..2936
Translation	MAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLELNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANVSSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSSEKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKYKVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPSEK

Target ORF information:

RefSeq Version	XM_024450263.1
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024450263.1

ORF Insert Sequence:

ATGGCACAGG AGGGCTTCAG CGCTGTGTTC ACACCTGATG GCCCCGTTCT GGGGGCTGTG 
GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC CTGTACCCCC CAAATATGAG CCCTACCTTC 
ATCAACATGT CTCAGGAGAA TGTGGACATG AGGGACTCTT ACCTGGGTTA CTCCACCGAG 
CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG GTCCTGGGGG CCCCCCGCTA CCAGCACACC 
GGGAAGGCTG TCATCTTCAC CCAGGTGTCC AGGCAATGGA GGATGAAGGC CGAAGTCACG 
GGGACTCAGA TCGGCTCCTA CTTCGGGGCC TCCCTCTGCT CCGTGGACGT AGACAGCGAC 
GGCAGCACCG ACCTGGTCCT CATCGGGGCC CCCCATTACT ACGAGCAGAC CCGAGGGGGC 
CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC 
TACGGGGAGC AGGGCCACCC CTGGGGTCGC TTTGGGGCGG CTCTGACAGT GCTGGGGGAT 
GTGAATGGGG ACAAGCTGAC AGACGTGGTC ATCGGGGCCC CAGGAGAGGA GGAGAACCGG 
GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG GGACCCAGCA TCAGCCCCTC CCACAGCCAG 
CGGATCGCGG GCTCCCAGCT CTCCTCCAGG CTGCAGTATT TTGGGCAGGC ACTGAGCGGG 
GGTCAAGACC TCACCCAGGA TGGACTGGTG GACCTGGCTG TGGGGGCCCG GGGCCAGGTG 
CTCCTGCTCA GGACCAGACC TGTGCTCTGG GTGGGGGTGA GCATGCAGTT CATACCTGCC 
GAGATCCCCA GGTCTGCGTT TGAGTGTCGG GAGCAGGTGG TCTCTGAGCA GACCCTGGTA 
CAGTCCAACA TCTGCCTTTA CATTGACAAA CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC 
CTCCAAAGCT CTGTGACCTT GGACCTGGCC CTCGACCCTG GCCGCCTGAG TCCCCGTGCC 
ACCTTCCAGG AAACAAAGAA CCGGAGTCTG AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA 
CACTGTGAAA ACTTCAACCT GCTGCTCCCG AGCTGCGTGG AGGACTCTGT GACCCCCATT 
ACCTTGCGTC TGAACTTCAC GCTGGTGGGC AAGCCCCTCC TTGCCTTCAG AAACCTGCGG 
CCTATGCTGG CCGCCGATGC TCAGAGATAC TTCACGGCCT CCCTACCCTT TGAGAAGAAC 
TGTGGAGCCG ACCATATCTG CCAGGACAAT CTCGGCATCT CCTTCAGCTT CCCAGGCTTG 
AAGTCCCTGC TGGTGGGGAG TAACCTGGAG CTGAACGCAG AAGTGATGGT GTGGAATGAC 
GGGGAAGACT CCTACGGAAC CACCATCACC TTCTCCCACC CCGCAGGACT GTCCTACCGC 
TACGTGGCAG AGGGCCAGAA ACAAGGGCAG CTGCGTTCCC TGCACCTGAC ATGTGACAGC 
GCCCCAGTTG GGAGCCAGGG CACCTGGAGC ACCAGCTGCA GAATCAACCA CCTCATCTTC 
CGTGGCGGCG CCCAGATCAC CTTCTTGGCT ACCTTTGACG TCTCCCCCAA GGCTGTCCTG 
GGAGACCGGC TGCTTCTGAC AGCCAATGTG AGCAGTGAGA ACAACACTCC CAGGACCAGC 
AAGACCACCT TCCAGCTGGA GCTCCCGGTG AAGTATGCTG TCTACACTGT GGTTAGCAGC 
CACGAACAAT TCACCAAATA CCTCAACTTC TCAGAGTCTG AGGAGAAGGA AAGCCATGTG 
GCCATGCACA GATACCAGGT CAATAACCTG GGACAGAGGG ACCTGCCTGT CAGCATCAAC 
TTCTGGGTGC CTGTGGAGCT GAACCAGGAG GCTGTGTGGA TGGATGTGGA GGTCTCCCAC 
CCCCAGAACC CATCCCTTCG GTGCTCCTCA GAGAAAATCG CACCCCCAGC ATCTGACTTC 
CTGGCGCACA TTCAGAAGAA TCCCGTGCTG GACTGCTCCA TTGCTGGCTG CCTGCGGTTC 
CGCTGTGACG TCCCCTCCTT CAGCGTCCAG GAGGAGCTGG ATTTCACCCT GAAGGGCAAC 
CTCAGCTTTG GCTGGGTCCG CCAGATATTG CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT 
GAAATTACGT TCGACACATC CGTGTACTCC CAGCTTCCAG GACAGGAGGC ATTTATGAGA 
GCTCAGACGA CAACGGTGCT GGAGAAGTAC AAGGTCCACA ACCCCACCCC CCTCATCGTA 
GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG GCACTCATCA CAGCGGTACT GTACAAAGTT 
GGCTTCTTCA AGCGTCAGTA CAAGGAAATG ATGGAGGAGG CAAATGGACA AATTGCCCCA 
GAAAACGGGA CACAGACCCC CAGCCCGCCC AGTGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGAX ( NM_001286375.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273304.1 Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 1, mRNA.

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CloneID	OHu12065
Clone ID Related Accession (Same CDS sequence)	NM_001286375.2 , NM_001286375.1
Accession Version	NM_001286375.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	integrin alpha-X isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA324567.1, BC038237.1, AC093520.4 and AW014280.1. On Jun 2, 2019 this sequence version replaced NM_001286375.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
ACTCCCCATT ACCCTCAGGA CAATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273304.1
CDS	80..3589
Translation	MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLELNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANVSSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSSEKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKYKVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPTPHYPQDNV

Target ORF information:

RefSeq Version	NM_001286375.2
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286375.2

ORF Insert Sequence:

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
ACTCCCCATT ACCCTCAGGA CAATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGAX ( NM_001286375.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001273304.1 Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 1, mRNA.

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CloneID	OHu12065
Clone ID Related Accession (Same CDS sequence)	NM_001286375.2 , NM_001286375.1
Accession Version	NM_001286375.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	integrin alpha-X isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA324567.1, BC038237.1, AC093520.4 and AW014280.1. On Nov 2, 2013 this sequence version replaced XM_005255314.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC038237.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA2146982 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
ACTCCCCATT ACCCTCAGGA CAATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001273304.1
CDS	122..3631
Translation	MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLELNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANVSSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSSEKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKYKVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPTPHYPQDNV

Target ORF information:

RefSeq Version	NM_001286375.1
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286375.1

ORF Insert Sequence:

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
ACTCCCCATT ACCCTCAGGA CAATGTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGAX ( NM_000887.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000878.2 Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 2, mRNA.

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CloneID	OHu20975
Clone ID Related Accession (Same CDS sequence)	NM_000887.5 , NM_000887.4
Accession Version	NM_000887.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	integrin alpha-X isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA324567.1, BC038237.1, M81695.1, AC093520.4 and AW014280.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000887.4. Transcript Variant: This variant (2) lacks an alternate intron in the 3' coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M81695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
AGTGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000878.2
CDS	80..3571
Translation	MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLELNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANVSSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSSEKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKYKVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPSEK

Target ORF information:

RefSeq Version	NM_000887.5
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000887.5

ORF Insert Sequence:

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
AGTGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITGAX ( NM_000887.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000878.2 Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 2, mRNA.

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CloneID	OHu20975
Clone ID Related Accession (Same CDS sequence)	NM_000887.5 , NM_000887.4
Accession Version	NM_000887.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3492bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	integrin alpha-X isoform 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA324567.1, BC038237.1, M81695.1, AC093520.4 and AW014280.1. This sequence is a reference standard in the RefSeqGene project. On Nov 2, 2013 this sequence version replaced NM_000887.3. Transcript Variant: This variant (2) lacks an alternate intron in the 3' coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M81695.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
AGTGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000878.2
CDS	122..3613
Translation	MTRTRAALLLFTALATSLGFNLDTEELTAFRVDSAGFGDSVVQYANSWVVVGAPQKITAANQTGGLYQCGYSTGACEPIGLQVPPEAVNMSLGLSLASTTSPSQLLACGPTVHHECGRNMYLTGLCFLLGPTQLTQRLPVSRQECPRQEQDIVFLIDGSGSISSRNFATMMNFVRAVISQFQRPSTQFSLMQFSNKFQTHFTFEEFRRSSNPLSLLASVHQLQGFTYTATAIQNVVHRLFHASYGARRDAAKILIVITDGKKEGDSLDYKDVIPMADAAGIIRYAIGVGLAFQNRNSWKELNDIASKPSQEHIFKVEDFDALKDIQNQLKEKIFAIEGTETTSSSSFELEMAQEGFSAVFTPDGPVLGAVGSFTWSGGAFLYPPNMSPTFINMSQENVDMRDSYLGYSTELALWKGVQSLVLGAPRYQHTGKAVIFTQVSRQWRMKAEVTGTQIGSYFGASLCSVDVDSDGSTDLVLIGAPHYYEQTRGGQVSVCPLPRGWRRWWCDAVLYGEQGHPWGRFGAALTVLGDVNGDKLTDVVIGAPGEEENRGAVYLFHGVLGPSISPSHSQRIAGSQLSSRLQYFGQALSGGQDLTQDGLVDLAVGARGQVLLLRTRPVLWVGVSMQFIPAEIPRSAFECREQVVSEQTLVQSNICLYIDKRSKNLLGSRDLQSSVTLDLALDPGRLSPRATFQETKNRSLSRVRVLGLKAHCENFNLLLPSCVEDSVTPITLRLNFTLVGKPLLAFRNLRPMLAADAQRYFTASLPFEKNCGADHICQDNLGISFSFPGLKSLLVGSNLELNAEVMVWNDGEDSYGTTITFSHPAGLSYRYVAEGQKQGQLRSLHLTCDSAPVGSQGTWSTSCRINHLIFRGGAQITFLATFDVSPKAVLGDRLLLTANVSSENNTPRTSKTTFQLELPVKYAVYTVVSSHEQFTKYLNFSESEEKESHVAMHRYQVNNLGQRDLPVSINFWVPVELNQEAVWMDVEVSHPQNPSLRCSSEKIAPPASDFLAHIQKNPVLDCSIAGCLRFRCDVPSFSVQEELDFTLKGNLSFGWVRQILQKKVSVVSVAEITFDTSVYSQLPGQEAFMRAQTTTVLEKYKVHNPTPLIVGSSIGGLLLLALITAVLYKVGFFKRQYKEMMEEANGQIAPENGTQTPSPPSEK

Target ORF information:

RefSeq Version	NM_000887.4
Organism	Homo sapiens(human)
Definition	Homo sapiens integrin subunit alpha X (ITGAX), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000887.4

ORF Insert Sequence:

ATGACCAGGA CCAGGGCAGC ACTCCTCCTG TTCACAGCCT TAGCAACTTC TCTAGGTTTC 
AACTTGGACA CAGAGGAGCT GACAGCCTTC CGTGTGGACA GCGCTGGGTT TGGAGACAGC 
GTGGTCCAGT ATGCCAACTC CTGGGTGGTG GTTGGAGCCC CCCAAAAGAT AACAGCTGCC 
AACCAAACGG GTGGCCTCTA CCAGTGTGGC TACAGCACTG GTGCCTGTGA GCCCATCGGC 
CTGCAGGTGC CCCCGGAGGC CGTGAACATG TCCCTGGGCC TGTCCCTGGC GTCTACCACC 
AGCCCTTCCC AGCTGCTGGC CTGCGGCCCC ACCGTGCACC ACGAGTGCGG GAGGAACATG 
TACCTCACCG GACTCTGCTT CCTCCTGGGC CCCACCCAGC TCACCCAGAG GCTCCCGGTG 
TCCAGGCAGG AGTGCCCAAG ACAGGAGCAG GACATTGTGT TCCTGATCGA TGGCTCAGGC 
AGCATCTCCT CCCGCAACTT TGCCACGATG ATGAACTTCG TGAGAGCTGT GATAAGCCAG 
TTCCAGAGAC CCAGCACCCA GTTTTCCCTG ATGCAGTTCT CCAACAAATT CCAAACACAC 
TTCACTTTCG AGGAATTCAG GCGCAGCTCA AACCCCCTCA GCCTGTTGGC TTCTGTTCAC 
CAGCTGCAAG GGTTTACATA CACGGCCACC GCCATCCAAA ATGTCGTGCA CCGATTGTTC 
CATGCCTCAT ATGGGGCCCG TAGGGATGCC GCCAAAATTC TCATTGTCAT CACTGATGGG 
AAGAAAGAAG GCGACAGCCT GGATTATAAG GATGTCATCC CCATGGCTGA TGCAGCAGGC 
ATCATCCGCT ATGCAATTGG GGTTGGATTA GCTTTTCAAA ACAGAAATTC TTGGAAAGAA 
TTAAATGACA TTGCATCGAA GCCCTCCCAG GAACACATAT TTAAAGTGGA GGACTTTGAT 
GCTCTGAAAG ATATTCAAAA CCAACTGAAG GAGAAGATCT TTGCCATTGA GGGTACGGAG 
ACCACAAGCA GTAGCTCCTT CGAATTGGAG ATGGCACAGG AGGGCTTCAG CGCTGTGTTC 
ACACCTGATG GCCCCGTTCT GGGGGCTGTG GGGAGCTTCA CCTGGTCTGG AGGTGCCTTC 
CTGTACCCCC CAAATATGAG CCCTACCTTC ATCAACATGT CTCAGGAGAA TGTGGACATG 
AGGGACTCTT ACCTGGGTTA CTCCACCGAG CTGGCCCTCT GGAAAGGGGT GCAGAGCCTG 
GTCCTGGGGG CCCCCCGCTA CCAGCACACC GGGAAGGCTG TCATCTTCAC CCAGGTGTCC 
AGGCAATGGA GGATGAAGGC CGAAGTCACG GGGACTCAGA TCGGCTCCTA CTTCGGGGCC 
TCCCTCTGCT CCGTGGACGT AGACAGCGAC GGCAGCACCG ACCTGGTCCT CATCGGGGCC 
CCCCATTACT ACGAGCAGAC CCGAGGGGGC CAGGTGTCTG TGTGTCCCTT GCCCAGGGGG 
TGGAGAAGGT GGTGGTGTGA TGCTGTTCTC TACGGGGAGC AGGGCCACCC CTGGGGTCGC 
TTTGGGGCGG CTCTGACAGT GCTGGGGGAT GTGAATGGGG ACAAGCTGAC AGACGTGGTC 
ATCGGGGCCC CAGGAGAGGA GGAGAACCGG GGTGCTGTCT ACCTGTTTCA CGGAGTCTTG 
GGACCCAGCA TCAGCCCCTC CCACAGCCAG CGGATCGCGG GCTCCCAGCT CTCCTCCAGG 
CTGCAGTATT TTGGGCAGGC ACTGAGCGGG GGTCAAGACC TCACCCAGGA TGGACTGGTG 
GACCTGGCTG TGGGGGCCCG GGGCCAGGTG CTCCTGCTCA GGACCAGACC TGTGCTCTGG 
GTGGGGGTGA GCATGCAGTT CATACCTGCC GAGATCCCCA GGTCTGCGTT TGAGTGTCGG 
GAGCAGGTGG TCTCTGAGCA GACCCTGGTA CAGTCCAACA TCTGCCTTTA CATTGACAAA 
CGTTCTAAGA ACCTGCTTGG GAGCCGTGAC CTCCAAAGCT CTGTGACCTT GGACCTGGCC 
CTCGACCCTG GCCGCCTGAG TCCCCGTGCC ACCTTCCAGG AAACAAAGAA CCGGAGTCTG 
AGCCGAGTCC GAGTCCTCGG GCTGAAGGCA CACTGTGAAA ACTTCAACCT GCTGCTCCCG 
AGCTGCGTGG AGGACTCTGT GACCCCCATT ACCTTGCGTC TGAACTTCAC GCTGGTGGGC 
AAGCCCCTCC TTGCCTTCAG AAACCTGCGG CCTATGCTGG CCGCCGATGC TCAGAGATAC 
TTCACGGCCT CCCTACCCTT TGAGAAGAAC TGTGGAGCCG ACCATATCTG CCAGGACAAT 
CTCGGCATCT CCTTCAGCTT CCCAGGCTTG AAGTCCCTGC TGGTGGGGAG TAACCTGGAG 
CTGAACGCAG AAGTGATGGT GTGGAATGAC GGGGAAGACT CCTACGGAAC CACCATCACC 
TTCTCCCACC CCGCAGGACT GTCCTACCGC TACGTGGCAG AGGGCCAGAA ACAAGGGCAG 
CTGCGTTCCC TGCACCTGAC ATGTGACAGC GCCCCAGTTG GGAGCCAGGG CACCTGGAGC 
ACCAGCTGCA GAATCAACCA CCTCATCTTC CGTGGCGGCG CCCAGATCAC CTTCTTGGCT 
ACCTTTGACG TCTCCCCCAA GGCTGTCCTG GGAGACCGGC TGCTTCTGAC AGCCAATGTG 
AGCAGTGAGA ACAACACTCC CAGGACCAGC AAGACCACCT TCCAGCTGGA GCTCCCGGTG 
AAGTATGCTG TCTACACTGT GGTTAGCAGC CACGAACAAT TCACCAAATA CCTCAACTTC 
TCAGAGTCTG AGGAGAAGGA AAGCCATGTG GCCATGCACA GATACCAGGT CAATAACCTG 
GGACAGAGGG ACCTGCCTGT CAGCATCAAC TTCTGGGTGC CTGTGGAGCT GAACCAGGAG 
GCTGTGTGGA TGGATGTGGA GGTCTCCCAC CCCCAGAACC CATCCCTTCG GTGCTCCTCA 
GAGAAAATCG CACCCCCAGC ATCTGACTTC CTGGCGCACA TTCAGAAGAA TCCCGTGCTG 
GACTGCTCCA TTGCTGGCTG CCTGCGGTTC CGCTGTGACG TCCCCTCCTT CAGCGTCCAG 
GAGGAGCTGG ATTTCACCCT GAAGGGCAAC CTCAGCTTTG GCTGGGTCCG CCAGATATTG 
CAGAAGAAGG TGTCGGTCGT GAGTGTGGCT GAAATTACGT TCGACACATC CGTGTACTCC 
CAGCTTCCAG GACAGGAGGC ATTTATGAGA GCTCAGACGA CAACGGTGCT GGAGAAGTAC 
AAGGTCCACA ACCCCACCCC CCTCATCGTA GGCAGCTCCA TTGGGGGTCT GTTGCTGCTG 
GCACTCATCA CAGCGGTACT GTACAAAGTT GGCTTCTTCA AGCGTCAGTA CAAGGAAATG 
ATGGAGGAGG CAAATGGACA AATTGCCCCA GAAAACGGGA CACAGACCCC CAGCCCGCCC 
AGTGAGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CD11c+ T-bet+ memory B cells: Immune maintenance during chronic infection and inflammation?
Cellular immunology3218-17(2017 Nov)
Winslow GM,Papillion AM,Kenderes KJ,Levack RC

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ITGAX cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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