INS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	INS
Entrez Gene ID	3630
Full Name	insulin
Synonyms	IDDM,IDDM1,IDDM2,ILPR,IRDN,MODY10
General protein information	Preferred Names insulin Names insulin preproinsulin proinsulin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.5
Summary	After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. A multitude of mutant alleles with phenotypic effects have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010].
Disorder MIM:	176730
Disorder Html:	Hyperproinsulinemia, 616214 (3); Maturity-onset diabetes of the young, type 10, 613370 (3); Diabetes mellitus, permanent neonatal, 606176 (3); Diabetes mellitus, insulin-dependent, 2, 125852 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000207.2	NP_000198.1	insulin preproprotein
NM_001185097.1	NP_001172026.1	insulin preproprotein
NM_001185097.2	NP_001172026.1	insulin preproprotein
NM_001291897.2	NP_001278826.1	insulin preproprotein
NM_000207.3	NP_000198.1	insulin preproprotein
NM_001185098.1	NP_001172027.1	insulin preproprotein
NM_001291897.1	NP_001278826.1	insulin preproprotein

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	INS	NP_001008996.1
Mus musculus (house mouse)	Ins2	NP_032413.1
Gallus gallus (chicken)	INS	NP_990553.1
Danio rerio (zebrafish)	ins	NP_571131.1
Xenopus tropicalis (tropical clawed frog)	ins	NP_001093706.1
Rattus norvegicus (Norway rat)	Ins2	NP_062003.1
Canis lupus familiaris (dog)	INS	NP_001123565.1
Homo sapiens (human)	INS	NP_000198.1

Gene Ontology

The following INS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the INS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22288	NM_001185098.1 Latest version!	Homo sapiens insulin (INS), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22288	NM_001185097.2 Latest version!	Homo sapiens insulin (INS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22288	NM_001291897.2 Latest version!	Homo sapiens insulin (INS), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22288	NM_000207.3 Latest version!	Homo sapiens insulin (INS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu22288	NM_001185097.1	Homo sapiens insulin (INS), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22288	NM_000207.2	Homo sapiens insulin (INS), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu22288	NM_001291897.1	Homo sapiens insulin (INS), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

INS ( NM_000207.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000198.1 Homo sapiens insulin (INS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22288
Clone ID Related Accession (Same CDS sequence)	NM_000207.2 , NM_001185097.1 , NM_001185097.2 , NM_001291897.2 , NM_000207.3 , NM_001185098.1 , NM_001291897.1
Accession Version	NM_000207.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	insulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005255.1 and BM510748.1. This sequence is a reference standard in the RefSeqGene project. On Jun 15, 2006 this sequence version replaced NM_000207.1. Transcript Variant: This variant (1) represents the shortest variant. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC005255.1, BU075674.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151405, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000198.1
CDS	60..392
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_000207.2
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin (INS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000207.2

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

INS ( NM_001185097.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172026.1 Homo sapiens insulin (INS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22288
Clone ID Related Accession (Same CDS sequence)	NM_000207.2 , NM_001185097.1 , NM_001185097.2 , NM_001291897.2 , NM_000207.3 , NM_001185098.1 , NM_001291897.1
Accession Version	NM_001185097.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	insulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP322143.1, AY899304.1 and BM510347.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BM876629.1, BQ787808.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151405, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172026.1
CDS	86..418
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_001185097.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin (INS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185097.1

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

INS ( NM_001185097.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172026.1 Homo sapiens insulin (INS), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu22288
Clone ID Related Accession (Same CDS sequence)	NM_000207.2 , NM_001185097.1 , NM_001185097.2 , NM_001291897.2 , NM_000207.3 , NM_001185098.1 , NM_001291897.1
Accession Version	NM_001185097.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	insulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP322143.1, AY899304.1 and BM510347.1. On Jun 2, 2019 this sequence version replaced NM_001185097.1. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU073287.1, BU783205.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151405, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172026.1
CDS	86..418
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_001185097.2
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin (INS), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185097.2

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

INS ( NM_001291897.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278826.1 Homo sapiens insulin (INS), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu22288
Clone ID Related Accession (Same CDS sequence)	NM_000207.2 , NM_001185097.1 , NM_001185097.2 , NM_001291897.2 , NM_000207.3 , NM_001185098.1 , NM_001291897.1
Accession Version	NM_001291897.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	insulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC132217.15 and BM510347.1. On Jun 2, 2019 this sequence version replaced NM_001291897.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BM512093.1, BI711659.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2146236, SAMEA2151405 [ECO:0000350] ##Evidence-Data-END##

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278826.1
CDS	120..452
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_001291897.2
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin (INS), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291897.2

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

INS ( NM_000207.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000198.1 Homo sapiens insulin (INS), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22288
Clone ID Related Accession (Same CDS sequence)	NM_000207.2 , NM_001185097.1 , NM_001185097.2 , NM_001291897.2 , NM_000207.3 , NM_001185098.1 , NM_001291897.1
Accession Version	NM_000207.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	insulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC005255.1 and BM510748.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000207.2. Transcript Variant: This variant (1) represents the shortest variant. All variants encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BU784533.1, BC005255.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151405, SAMEA2153347 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000198.1
CDS	60..392
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_000207.3
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin (INS), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000207.3

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

INS ( NM_001185098.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001172027.1 Homo sapiens insulin (INS), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22288
Clone ID Related Accession (Same CDS sequence)	NM_000207.2 , NM_001185097.1 , NM_001185097.2 , NM_001291897.2 , NM_000207.3 , NM_001185098.1 , NM_001291897.1
Accession Version	NM_001185098.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Homo sapiens(human)
Product	insulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP322143.1, AC132217.15 and BM510347.1. Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP322143.1, BM352532.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMEA2151405 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## regulatory uORF :: PMID: 20628762 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001172027.1
CDS	239..571
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_001185098.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin (INS), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001185098.1

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

INS ( NM_001291897.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278826.1 Homo sapiens insulin (INS), transcript variant 4, mRNA.

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CloneID	OHu22288
Clone ID Related Accession (Same CDS sequence)	NM_000207.2 , NM_001185097.1 , NM_001185097.2 , NM_001291897.2 , NM_000207.3 , NM_001185098.1 , NM_001291897.1
Accession Version	NM_001291897.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	insulin preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC132217.15 and BM510347.1. Transcript Variant: This variant (4) differs in the 5' UTR, compared to variant 1. All variants encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BI711149.1, BT006808.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA2146236, SAMEA2151405 [ECO:0000350] ##Evidence-Data-END##

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278826.1
CDS	120..452
Translation	MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSICSLYQLENYCN

Target ORF information:

RefSeq Version	NM_001291897.1
Organism	Homo sapiens(human)
Definition	Homo sapiens insulin (INS), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291897.1

ORF Insert Sequence:

ATGGCCCTGT GGATGCGCCT CCTGCCCCTG CTGGCGCTGC TGGCCCTCTG GGGACCTGAC 
CCAGCCGCAG CCTTTGTGAA CCAACACCTG TGCGGCTCAC ACCTGGTGGA AGCTCTCTAC 
CTAGTGTGCG GGGAACGAGG CTTCTTCTAC ACACCCAAGA CCCGCCGGGA GGCAGAGGAC 
CTGCAGGTGG GGCAGGTGGA GCTGGGCGGG GGCCCTGGTG CAGGCAGCCT GCAGCCCTTG 
GCCCTGGAGG GGTCCCTGCA GAAGCGTGGC ATTGTGGAAC AATGCTGTAC CAGCATCTGC 
TCCCTCTACC AGCTGGAGAA CTACTGCAAC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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INS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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