ACADSB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACADSB
Entrez Gene ID	36
Full Name	acyl-CoA dehydrogenase short/branched chain
Synonyms	2-MEBCAD,ACAD7,SBCAD
General protein information	Preferred Names acyl-CoA dehydrogenase short/branched chain Names short/branched chain specific acyl-CoA dehydrogenase, mitochondrial 2-methyl branched chain acyl-CoA dehydrogenase 2-methylbutyryl-coenzyme A dehydrogenase acyl-Coenzyme A dehydrogenase, short/branched chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 10 Map Location: 10q26.13
Summary	Short/branched chain acyl-CoA dehydrogenase(ACADSB) is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. Substrate specificity is the primary characteristic used to define members of this gene family. The ACADSB gene product has the greatest activity towards the short branched chain acyl-CoA derivative, (S)-2-methylbutyryl-CoA, but also reacts significantly with other 2-methyl branched chain substrates and with short straight chain acyl-CoAs. The cDNA encodes for a mitochondrial precursor protein which is cleaved upon mitochondrial import and predicted to yield a mature peptide of approximately 43.7-KDa. [provided by RefSeq, Jul 2008].
Disorder MIM:	600301
Disorder Html:	2-methylbutyrylglycinuria, 610006 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001609.3	NP_001600.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1 precursor
NM_001330174.2	NP_001317103.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 2
NM_001609.4	NP_001600.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1 precursor
NM_001330174.1	NP_001317103.1	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 2

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	acadsb	NP_001005724.1
Mus musculus (house mouse)	Acadsb	NP_080102.1
Drosophila melanogaster (fruit fly)	CG3902	NP_649069.2
Caenorhabditis elegans (roundworm)	acdh-3	NP_491859.1
Caenorhabditis elegans (roundworm)	acdh-1	NP_491871.1
Magnaporthe oryzae (rice blast fungus)	MGG_08690	XP_003719291.1
Neurospora crassa	NCU06543	XP_962250.1
Homo sapiens (human)	ACADSB	NP_001600.1
Canis lupus familiaris (dog)	ACADSB	XP_535048.5
Bos taurus (cattle)	ACADSB	NP_001017933.1
Rattus norvegicus (Norway rat)	Acadsb	NP_037216.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009783	XP_318878.3
Gallus gallus (chicken)	ACADSB	NP_001026411.1
Pan troglodytes (chimpanzee)	ACADSB	XP_001151318.2
	ACADSB	XP_001104844.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Yang X, Coulombe-Huntington J, Kang S, Sheynkman GM, Hao T, Richardson A, Sun S, Yang F, Shen YA, Murray RR, Spirohn K, Begg BE, Duran-Frigola M, MacWilliams A, Pevzner SJ, Zhong Q, Trigg SA, Tam S, Ghamsari L, Sahni N, Yi S, Rodriguez MD, Balcha D, Tan G, Costanzo M, Andrews B, Boone C, Zhou XJ, Salehi-Ashtiani K, Charloteaux B, Chen AA, Calderwood MA, Aloy P, Roth FP, Hill DE, Iakoucheva LM, Xia Y, Vidal M
Cell164(4)805-17(2016 Feb)

Prevalence and mutation analysis of short/branched chain acyl-CoA dehydrogenase deficiency (SBCADD) detected on newborn screening in Wisconsin.
Van Calcar SC, Baker MW, Williams P, Jones SA, Xiong B, Thao MC, Lee S, Yang MK, Rice GM, Rhead W, Vockley J, Hoffman G, Durkin MS
Molecular genetics and metabolism110(1-2)111-5(2013 Sep-Oct)

Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.
Calvo SE, Compton AG, Hershman SG, Lim SC, Lieber DS, Tucker EJ, Laskowski A, Garone C, Liu S, Jaffe DB, Christodoulou J, Fletcher JM, Bruno DL, Goldblatt J, Dimauro S, Thorburn DR, Mootha VK
Science translational medicine4(118)118ra10(2012 Jan)

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

the c.1165 A>G mutation before knowing whether the optimal screening cut-off would minimize true positives or false negatives for SBCADD associated with this mutation.
Title: Prevalence and mutation analysis of short/branched chain acyl-CoA dehydrogenase deficiency (SBCADD) detected on newborn screening in Wisconsin.

Strong candidate gene for mitochondrial disease, based on recessive mutations detected in infantile patients
Title: Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

These findings confirm that SBCAD deficiency can be identified through newborn screening by acylcarnitine analysis.
Title: Characterization of new ACADSB gene sequence mutations and clinical implications in patients with 2-methylbutyrylglycinuria identified by newborn screening.

Observational study, meta-analysis, and genome-wide association study of gene-disease association. (HuGE Navigator)
Title: Genome-wide association study of circulating vitamin D levels.

The following ACADSB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACADSB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu100441	NM_001330174.2 Latest version!	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
OHu18915	NM_001609.4 Latest version!	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
View Old Accession Versions >>
OHu18915	NM_001609.3	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu100441	NM_001330174.1	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACADSB ( NM_001609.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001600.1 Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu18915
Clone ID Related Accession (Same CDS sequence)	NM_001609.3 , NM_001609.4
Accession Version	NM_001609.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-17
Organism	Homo sapiens(human)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA964130.1, BC013756.1, U12778.1 and AC012391.10. This sequence is a reference standard in the RefSeqGene project. On May 15, 2006 this sequence version replaced NM_001609.2. Sequence Note: The 3' UTR extension represented by the RefSeq transcript record was derived from genomic sequence data to optimize consistency to the reference genome assembly. The extent of the UTR extension and the location of the polyA site was based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL831821.2, SRR1660805.182479.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCC TGGCAGTGCG GTTGCTGCGC GGCAGCAGGC TGCTAAGAAG AAATTTCCTG 
ACTTGTTTGT CTTCTTGGAA GATTCCTCCT CATGTCTCAA AATCTTCCCA GTCAGAAGCT 
CTACTCAATA TAACAAATAA TGGAATACAC TTTGCTCCCC TGCAAACATT TACAGATGAG 
GAAATGATGA TAAAGAGTTC AGTTAAAAAA TTTGCTCAGG AACAAATTGC ACCTTTGGTT 
TCAACCATGG ATGAAAATTC GAAAATGGAG AAATCAGTAA TACAAGGATT ATTTCAACAA 
GGGTTGATGG GTATTGAAGT TGACCCAGAA TATGGAGGCA CAGGAGCTTC ATTTTTATCC 
ACTGTGCTCG TGATAGAGGA ATTAGCCAAA GTTGATGCAT CTGTGGCTGT CTTTTGTGAG 
ATCCAGAACA CATTAATTAA CACACTGATT AGAAAACATG GAACAGAAGA ACAAAAGGCC 
ACCTATTTGC CTCAGCTCAC TACAGAAAAA GTAGGAAGTT TCTGCCTTTC AGAGGCTGGA 
GCAGGTAGTG ACTCATTTGC TTTGAAGACC AGAGCTGATA AAGAGGGAGA TTATTATGTC 
CTCAATGGAT CAAAGATGTG GATCAGCAGT GCTGAGCACG CAGGGCTCTT TCTGGTGATG 
GCAAATGTAG ACCCTACCAT TGGATATAAG GGAATTACCT CCTTCTTAGT AGATCGTGAT 
ACTCCGGGCC TTCATATAGG GAAACCTGAA AACAAATTGG GGCTCAGAGC TTCTTCCACC 
TGCCCGTTAA CATTCGAAAA TGTCAAGGTT CCAGAAGCCA ATATCTTGGG ACAAATTGGA 
CATGGCTATA AGTATGCCAT AGGGAGTCTC AATGAAGGTA GAATAGGAAT TGCTGCACAG 
ATGCTGGGAC TGGCGCAAGG ATGTTTTGAC TACACTATTC CATATATTAA AGAAAGGATA 
CAATTTGGCA AAAGACTATT TGATTTTCAG GGCCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCAAG ATTACTAACA TACAATGCTG CTAGGCTTTT AGAAGCTGGA 
AAGCCATTCA TAAAAGAAGC GTCAATGGCC AAATACTATG CATCAGAGAT TGCAGGACAA 
ACAACGAGTA AATGTATCGA GTGGATGGGG GGAGTAGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATACT TCCGAGATGC AAAGATTGGT ACGATATATG AAGGAGCTTC CAACATCCAG 
TTGAACACCA TTGCAAAGCA TATCGATGCA GAATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001600.1
CDS	118..1416
Translation	MEGLAVRLLRGSRLLRRNFLTCLSSWKIPPHVSKSSQSEALLNITNNGIHFAPLQTFTDEEMMIKSSVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGLMGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCEIQNTLINTLIRKHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISSAEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLTYNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHIDAEY

Target ORF information:

RefSeq Version	NM_001609.3
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001609.3

ORF Insert Sequence:

ATGGAGGGCC TGGCAGTGCG GTTGCTGCGC GGCAGCAGGC TGCTAAGAAG AAATTTCCTG 
ACTTGTTTGT CTTCTTGGAA GATTCCTCCT CATGTCTCAA AATCTTCCCA GTCAGAAGCT 
CTACTCAATA TAACAAATAA TGGAATACAC TTTGCTCCCC TGCAAACATT TACAGATGAG 
GAAATGATGA TAAAGAGTTC AGTTAAAAAA TTTGCTCAGG AACAAATTGC ACCTTTGGTT 
TCAACCATGG ATGAAAATTC GAAAATGGAG AAATCAGTAA TACAAGGATT ATTTCAACAA 
GGGTTGATGG GTATTGAAGT TGACCCAGAA TATGGAGGCA CAGGAGCTTC ATTTTTATCC 
ACTGTGCTCG TGATAGAGGA ATTAGCCAAA GTTGATGCAT CTGTGGCTGT CTTTTGTGAG 
ATCCAGAACA CATTAATTAA CACACTGATT AGAAAACATG GAACAGAAGA ACAAAAGGCC 
ACCTATTTGC CTCAGCTCAC TACAGAAAAA GTAGGAAGTT TCTGCCTTTC AGAGGCTGGA 
GCAGGTAGTG ACTCATTTGC TTTGAAGACC AGAGCTGATA AAGAGGGAGA TTATTATGTC 
CTCAATGGAT CAAAGATGTG GATCAGCAGT GCTGAGCACG CAGGGCTCTT TCTGGTGATG 
GCAAATGTAG ACCCTACCAT TGGATATAAG GGAATTACCT CCTTCTTAGT AGATCGTGAT 
ACTCCGGGCC TTCATATAGG GAAACCTGAA AACAAATTGG GGCTCAGAGC TTCTTCCACC 
TGCCCGTTAA CATTCGAAAA TGTCAAGGTT CCAGAAGCCA ATATCTTGGG ACAAATTGGA 
CATGGCTATA AGTATGCCAT AGGGAGTCTC AATGAAGGTA GAATAGGAAT TGCTGCACAG 
ATGCTGGGAC TGGCGCAAGG ATGTTTTGAC TACACTATTC CATATATTAA AGAAAGGATA 
CAATTTGGCA AAAGACTATT TGATTTTCAG GGCCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCAAG ATTACTAACA TACAATGCTG CTAGGCTTTT AGAAGCTGGA 
AAGCCATTCA TAAAAGAAGC GTCAATGGCC AAATACTATG CATCAGAGAT TGCAGGACAA 
ACAACGAGTA AATGTATCGA GTGGATGGGG GGAGTAGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATACT TCCGAGATGC AAAGATTGGT ACGATATATG AAGGAGCTTC CAACATCCAG 
TTGAACACCA TTGCAAAGCA TATCGATGCA GAATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADSB ( NM_001330174.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317103.1 Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu100441
Clone ID Related Accession (Same CDS sequence)	NM_001330174.2 , NM_001330174.1
Accession Version	NM_001330174.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC412570.1, H56072.1, BQ312626.1, AW408417.1, AC012391.10 and BC032395.1. On Jun 2, 2019 this sequence version replaced NM_001330174.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.172950.1, SRR1803612.231941.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTATTG AAGTTGACCC AGAATATGGA GGCACAGGAG CTTCATTTTT ATCCACTGTG 
CTCGTGATAG AGGAATTAGC CAAAGTTGAT GCATCTGTGG CTGTCTTTTG TGAGATCCAG 
AACACATTAA TTAACACACT GATTAGAAAA CATGGAACAG AAGAACAAAA GGCCACCTAT 
TTGCCTCAGC TCACTACAGA AAAAGTAGGA AGTTTCTGCC TTTCAGAGGC TGGAGCAGGT 
AGTGACTCAT TTGCTTTGAA GACCAGAGCT GATAAAGAGG GAGATTATTA TGTCCTCAAT 
GGATCAAAGA TGTGGATCAG CAGTGCTGAG CACGCAGGGC TCTTTCTGGT GATGGCAAAT 
GTAGACCCTA CCATTGGATA TAAGGGAATT ACCTCCTTCT TAGTAGATCG TGATACTCCG 
GGCCTTCATA TAGGGAAACC TGAAAACAAA TTGGGGCTCA GAGCTTCTTC CACCTGCCCG 
TTAACATTCG AAAATGTCAA GGTTCCAGAA GCCAATATCT TGGGACAAAT TGGACATGGC 
TATAAGTATG CCATAGGGAG TCTCAATGAA GGTAGAATAG GAATTGCTGC ACAGATGCTG 
GGACTGGCGC AAGGATGTTT TGACTACACT ATTCCATATA TTAAAGAAAG GATACAATTT 
GGCAAAAGAC TATTTGATTT TCAGGGCCTC CAACACCAAG TGGCTCACGT GGCCACCCAG 
CTGGAAGCTG CAAGATTACT AACATACAAT GCTGCTAGGC TTTTAGAAGC TGGAAAGCCA 
TTCATAAAAG AAGCGTCAAT GGCCAAATAC TATGCATCAG AGATTGCAGG ACAAACAACG 
AGTAAATGTA TCGAGTGGAT GGGGGGAGTA GGCTACACCA AAGATTACCC TGTGGAGAAA 
TACTTCCGAG ATGCAAAGAT TGGTACGATA TATGAAGGAG CTTCCAACAT CCAGTTGAAC 
ACCATTGCAA AGCATATCGA TGCAGAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317103.1
CDS	230..1222
Translation	MGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCEIQNTLINTLIRKHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISSAEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLTYNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHIDAEY

Target ORF information:

RefSeq Version	NM_001330174.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330174.2

ORF Insert Sequence:

ATGGGTATTG AAGTTGACCC AGAATATGGA GGCACAGGAG CTTCATTTTT ATCCACTGTG 
CTCGTGATAG AGGAATTAGC CAAAGTTGAT GCATCTGTGG CTGTCTTTTG TGAGATCCAG 
AACACATTAA TTAACACACT GATTAGAAAA CATGGAACAG AAGAACAAAA GGCCACCTAT 
TTGCCTCAGC TCACTACAGA AAAAGTAGGA AGTTTCTGCC TTTCAGAGGC TGGAGCAGGT 
AGTGACTCAT TTGCTTTGAA GACCAGAGCT GATAAAGAGG GAGATTATTA TGTCCTCAAT 
GGATCAAAGA TGTGGATCAG CAGTGCTGAG CACGCAGGGC TCTTTCTGGT GATGGCAAAT 
GTAGACCCTA CCATTGGATA TAAGGGAATT ACCTCCTTCT TAGTAGATCG TGATACTCCG 
GGCCTTCATA TAGGGAAACC TGAAAACAAA TTGGGGCTCA GAGCTTCTTC CACCTGCCCG 
TTAACATTCG AAAATGTCAA GGTTCCAGAA GCCAATATCT TGGGACAAAT TGGACATGGC 
TATAAGTATG CCATAGGGAG TCTCAATGAA GGTAGAATAG GAATTGCTGC ACAGATGCTG 
GGACTGGCGC AAGGATGTTT TGACTACACT ATTCCATATA TTAAAGAAAG GATACAATTT 
GGCAAAAGAC TATTTGATTT TCAGGGCCTC CAACACCAAG TGGCTCACGT GGCCACCCAG 
CTGGAAGCTG CAAGATTACT AACATACAAT GCTGCTAGGC TTTTAGAAGC TGGAAAGCCA 
TTCATAAAAG AAGCGTCAAT GGCCAAATAC TATGCATCAG AGATTGCAGG ACAAACAACG 
AGTAAATGTA TCGAGTGGAT GGGGGGAGTA GGCTACACCA AAGATTACCC TGTGGAGAAA 
TACTTCCGAG ATGCAAAGAT TGGTACGATA TATGAAGGAG CTTCCAACAT CCAGTTGAAC 
ACCATTGCAA AGCATATCGA TGCAGAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADSB ( NM_001330174.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317103.1 Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu100441
Clone ID Related Accession (Same CDS sequence)	NM_001330174.2 , NM_001330174.1
Accession Version	NM_001330174.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 993bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA964130.1, DC412570.1, H56072.1, BQ312626.1, AW408417.1, AC012391.10 and BC032395.1. On Aug 18, 2016 this sequence version replaced XM_017016194.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.231941.1, SRR1660805.3126.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGGTATTG AAGTTGACCC AGAATATGGA GGCACAGGAG CTTCATTTTT ATCCACTGTG 
CTCGTGATAG AGGAATTAGC CAAAGTTGAT GCATCTGTGG CTGTCTTTTG TGAGATCCAG 
AACACATTAA TTAACACACT GATTAGAAAA CATGGAACAG AAGAACAAAA GGCCACCTAT 
TTGCCTCAGC TCACTACAGA AAAAGTAGGA AGTTTCTGCC TTTCAGAGGC TGGAGCAGGT 
AGTGACTCAT TTGCTTTGAA GACCAGAGCT GATAAAGAGG GAGATTATTA TGTCCTCAAT 
GGATCAAAGA TGTGGATCAG CAGTGCTGAG CACGCAGGGC TCTTTCTGGT GATGGCAAAT 
GTAGACCCTA CCATTGGATA TAAGGGAATT ACCTCCTTCT TAGTAGATCG TGATACTCCG 
GGCCTTCATA TAGGGAAACC TGAAAACAAA TTGGGGCTCA GAGCTTCTTC CACCTGCCCG 
TTAACATTCG AAAATGTCAA GGTTCCAGAA GCCAATATCT TGGGACAAAT TGGACATGGC 
TATAAGTATG CCATAGGGAG TCTCAATGAA GGTAGAATAG GAATTGCTGC ACAGATGCTG 
GGACTGGCGC AAGGATGTTT TGACTACACT ATTCCATATA TTAAAGAAAG GATACAATTT 
GGCAAAAGAC TATTTGATTT TCAGGGCCTC CAACACCAAG TGGCTCACGT GGCCACCCAG 
CTGGAAGCTG CAAGATTACT AACATACAAT GCTGCTAGGC TTTTAGAAGC TGGAAAGCCA 
TTCATAAAAG AAGCGTCAAT GGCCAAATAC TATGCATCAG AGATTGCAGG ACAAACAACG 
AGTAAATGTA TCGAGTGGAT GGGGGGAGTA GGCTACACCA AAGATTACCC TGTGGAGAAA 
TACTTCCGAG ATGCAAAGAT TGGTACGATA TATGAAGGAG CTTCCAACAT CCAGTTGAAC 
ACCATTGCAA AGCATATCGA TGCAGAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317103.1
CDS	323..1315
Translation	MGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCEIQNTLINTLIRKHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISSAEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLTYNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHIDAEY

Target ORF information:

RefSeq Version	NM_001330174.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330174.1

ORF Insert Sequence:

ATGGGTATTG AAGTTGACCC AGAATATGGA GGCACAGGAG CTTCATTTTT ATCCACTGTG 
CTCGTGATAG AGGAATTAGC CAAAGTTGAT GCATCTGTGG CTGTCTTTTG TGAGATCCAG 
AACACATTAA TTAACACACT GATTAGAAAA CATGGAACAG AAGAACAAAA GGCCACCTAT 
TTGCCTCAGC TCACTACAGA AAAAGTAGGA AGTTTCTGCC TTTCAGAGGC TGGAGCAGGT 
AGTGACTCAT TTGCTTTGAA GACCAGAGCT GATAAAGAGG GAGATTATTA TGTCCTCAAT 
GGATCAAAGA TGTGGATCAG CAGTGCTGAG CACGCAGGGC TCTTTCTGGT GATGGCAAAT 
GTAGACCCTA CCATTGGATA TAAGGGAATT ACCTCCTTCT TAGTAGATCG TGATACTCCG 
GGCCTTCATA TAGGGAAACC TGAAAACAAA TTGGGGCTCA GAGCTTCTTC CACCTGCCCG 
TTAACATTCG AAAATGTCAA GGTTCCAGAA GCCAATATCT TGGGACAAAT TGGACATGGC 
TATAAGTATG CCATAGGGAG TCTCAATGAA GGTAGAATAG GAATTGCTGC ACAGATGCTG 
GGACTGGCGC AAGGATGTTT TGACTACACT ATTCCATATA TTAAAGAAAG GATACAATTT 
GGCAAAAGAC TATTTGATTT TCAGGGCCTC CAACACCAAG TGGCTCACGT GGCCACCCAG 
CTGGAAGCTG CAAGATTACT AACATACAAT GCTGCTAGGC TTTTAGAAGC TGGAAAGCCA 
TTCATAAAAG AAGCGTCAAT GGCCAAATAC TATGCATCAG AGATTGCAGG ACAAACAACG 
AGTAAATGTA TCGAGTGGAT GGGGGGAGTA GGCTACACCA AAGATTACCC TGTGGAGAAA 
TACTTCCGAG ATGCAAAGAT TGGTACGATA TATGAAGGAG CTTCCAACAT CCAGTTGAAC 
ACCATTGCAA AGCATATCGA TGCAGAATAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADSB ( NM_001609.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_001600.1 Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 1, mRNA.

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CloneID	OHu18915
Clone ID Related Accession (Same CDS sequence)	NM_001609.3 , NM_001609.4
Accession Version	NM_001609.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1299bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	short/branched chain specific acyl-CoA dehydrogenase, mitochondrial isoform 1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA964130.1, BC013756.1, U12778.1 and AC012391.10. On Aug 31, 2019 this sequence version replaced NM_001609.3. Sequence Note: The 3' UTR extension represented by the RefSeq transcript record was derived from genomic sequence data to optimize consistency to the reference genome assembly. The extent of the UTR extension and the location of the polyA site was based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL831821.2, SRR1803614.134673.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGGCC TGGCAGTGCG GTTGCTGCGC GGCAGCAGGC TGCTAAGAAG AAATTTCCTG 
ACTTGTTTGT CTTCTTGGAA GATTCCTCCT CATGTCTCAA AATCTTCCCA GTCAGAAGCT 
CTACTCAATA TAACAAATAA TGGAATACAC TTTGCTCCCC TGCAAACATT TACAGATGAG 
GAAATGATGA TAAAGAGTTC AGTTAAAAAA TTTGCTCAGG AACAAATTGC ACCTTTGGTT 
TCAACCATGG ATGAAAATTC GAAAATGGAG AAATCAGTAA TACAAGGATT ATTTCAACAA 
GGGTTGATGG GTATTGAAGT TGACCCAGAA TATGGAGGCA CAGGAGCTTC ATTTTTATCC 
ACTGTGCTCG TGATAGAGGA ATTAGCCAAA GTTGATGCAT CTGTGGCTGT CTTTTGTGAG 
ATCCAGAACA CATTAATTAA CACACTGATT AGAAAACATG GAACAGAAGA ACAAAAGGCC 
ACCTATTTGC CTCAGCTCAC TACAGAAAAA GTAGGAAGTT TCTGCCTTTC AGAGGCTGGA 
GCAGGTAGTG ACTCATTTGC TTTGAAGACC AGAGCTGATA AAGAGGGAGA TTATTATGTC 
CTCAATGGAT CAAAGATGTG GATCAGCAGT GCTGAGCACG CAGGGCTCTT TCTGGTGATG 
GCAAATGTAG ACCCTACCAT TGGATATAAG GGAATTACCT CCTTCTTAGT AGATCGTGAT 
ACTCCGGGCC TTCATATAGG GAAACCTGAA AACAAATTGG GGCTCAGAGC TTCTTCCACC 
TGCCCGTTAA CATTCGAAAA TGTCAAGGTT CCAGAAGCCA ATATCTTGGG ACAAATTGGA 
CATGGCTATA AGTATGCCAT AGGGAGTCTC AATGAAGGTA GAATAGGAAT TGCTGCACAG 
ATGCTGGGAC TGGCGCAAGG ATGTTTTGAC TACACTATTC CATATATTAA AGAAAGGATA 
CAATTTGGCA AAAGACTATT TGATTTTCAG GGCCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCAAG ATTACTAACA TACAATGCTG CTAGGCTTTT AGAAGCTGGA 
AAGCCATTCA TAAAAGAAGC GTCAATGGCC AAATACTATG CATCAGAGAT TGCAGGACAA 
ACAACGAGTA AATGTATCGA GTGGATGGGG GGAGTAGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATACT TCCGAGATGC AAAGATTGGT ACGATATATG AAGGAGCTTC CAACATCCAG 
TTGAACACCA TTGCAAAGCA TATCGATGCA GAATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001600.1
CDS	25..1323
Translation	MEGLAVRLLRGSRLLRRNFLTCLSSWKIPPHVSKSSQSEALLNITNNGIHFAPLQTFTDEEMMIKSSVKKFAQEQIAPLVSTMDENSKMEKSVIQGLFQQGLMGIEVDPEYGGTGASFLSTVLVIEELAKVDASVAVFCEIQNTLINTLIRKHGTEEQKATYLPQLTTEKVGSFCLSEAGAGSDSFALKTRADKEGDYYVLNGSKMWISSAEHAGLFLVMANVDPTIGYKGITSFLVDRDTPGLHIGKPENKLGLRASSTCPLTFENVKVPEANILGQIGHGYKYAIGSLNEGRIGIAAQMLGLAQGCFDYTIPYIKERIQFGKRLFDFQGLQHQVAHVATQLEAARLLTYNAARLLEAGKPFIKEASMAKYYASEIAGQTTSKCIEWMGGVGYTKDYPVEKYFRDAKIGTIYEGASNIQLNTIAKHIDAEY

Target ORF information:

RefSeq Version	NM_001609.4
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase short/branched chain (ACADSB), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001609.4

ORF Insert Sequence:

ATGGAGGGCC TGGCAGTGCG GTTGCTGCGC GGCAGCAGGC TGCTAAGAAG AAATTTCCTG 
ACTTGTTTGT CTTCTTGGAA GATTCCTCCT CATGTCTCAA AATCTTCCCA GTCAGAAGCT 
CTACTCAATA TAACAAATAA TGGAATACAC TTTGCTCCCC TGCAAACATT TACAGATGAG 
GAAATGATGA TAAAGAGTTC AGTTAAAAAA TTTGCTCAGG AACAAATTGC ACCTTTGGTT 
TCAACCATGG ATGAAAATTC GAAAATGGAG AAATCAGTAA TACAAGGATT ATTTCAACAA 
GGGTTGATGG GTATTGAAGT TGACCCAGAA TATGGAGGCA CAGGAGCTTC ATTTTTATCC 
ACTGTGCTCG TGATAGAGGA ATTAGCCAAA GTTGATGCAT CTGTGGCTGT CTTTTGTGAG 
ATCCAGAACA CATTAATTAA CACACTGATT AGAAAACATG GAACAGAAGA ACAAAAGGCC 
ACCTATTTGC CTCAGCTCAC TACAGAAAAA GTAGGAAGTT TCTGCCTTTC AGAGGCTGGA 
GCAGGTAGTG ACTCATTTGC TTTGAAGACC AGAGCTGATA AAGAGGGAGA TTATTATGTC 
CTCAATGGAT CAAAGATGTG GATCAGCAGT GCTGAGCACG CAGGGCTCTT TCTGGTGATG 
GCAAATGTAG ACCCTACCAT TGGATATAAG GGAATTACCT CCTTCTTAGT AGATCGTGAT 
ACTCCGGGCC TTCATATAGG GAAACCTGAA AACAAATTGG GGCTCAGAGC TTCTTCCACC 
TGCCCGTTAA CATTCGAAAA TGTCAAGGTT CCAGAAGCCA ATATCTTGGG ACAAATTGGA 
CATGGCTATA AGTATGCCAT AGGGAGTCTC AATGAAGGTA GAATAGGAAT TGCTGCACAG 
ATGCTGGGAC TGGCGCAAGG ATGTTTTGAC TACACTATTC CATATATTAA AGAAAGGATA 
CAATTTGGCA AAAGACTATT TGATTTTCAG GGCCTCCAAC ACCAAGTGGC TCACGTGGCC 
ACCCAGCTGG AAGCTGCAAG ATTACTAACA TACAATGCTG CTAGGCTTTT AGAAGCTGGA 
AAGCCATTCA TAAAAGAAGC GTCAATGGCC AAATACTATG CATCAGAGAT TGCAGGACAA 
ACAACGAGTA AATGTATCGA GTGGATGGGG GGAGTAGGCT ACACCAAAGA TTACCCTGTG 
GAGAAATACT TCCGAGATGC AAAGATTGGT ACGATATATG AAGGAGCTTC CAACATCCAG 
TTGAACACCA TTGCAAAGCA TATCGATGCA GAATACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread Expansion of Protein Interaction Capabilities by Alternative Splicing.
Cell164(4)805-17(2016 Feb)
Yang X,Coulombe-Huntington J,Kang S,Sheynkman GM,Hao T,Richardson A,Sun S,Yang F,Shen YA,Murray RR,Spirohn K,Begg BE,Duran-Frigola M,MacWilliams A,Pevzner SJ,Zhong Q,Trigg SA,Tam S,Ghamsari L,Sahni N,Yi S,Rodriguez MD,Balcha D,Tan G,Costanzo M,Andrews B,Boone C,Zhou XJ,Salehi-Ashtiani K,Charloteaux B,Chen AA,Calderwood MA,Aloy P,Roth FP,Hill DE,Iakoucheva LM,Xia Y,Vidal M

Prevalence and mutation analysis of short/branched chain acyl-CoA dehydrogenase deficiency (SBCADD) detected on newborn screening in Wisconsin.
Molecular genetics and metabolism110(1-2)111-5(2013 Sep-Oct)
Van Calcar SC,Baker MW,Williams P,Jones SA,Xiong B,Thao MC,Lee S,Yang MK,Rice GM,Rhead W,Vockley J,Hoffman G,Durkin MS

Molecular diagnosis of infantile mitochondrial disease with targeted next-generation sequencing.
Science translational medicine4(118)118ra10(2012 Jan)
Calvo SE,Compton AG,Hershman SG,Lim SC,Lieber DS,Tucker EJ,Laskowski A,Garone C,Liu S,Jaffe DB,Christodoulou J,Fletcher JM,Bruno DL,Goldblatt J,Dimauro S,Thorburn DR,Mootha VK

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

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ACADSB cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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