IGSF1 cDNA ORF clone, Homo sapiens(human)

The following IGSF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IGSF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu22594 NM_001555.4
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu16613 NM_205833.3
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu16613 NM_001170963.1
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $139.30
$199.00
OHu108233 NM_205833.4
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $139.30
$199.00
OHu107209 NM_001555.5
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu108258 NM_001170962.2
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu22998 NM_001170962.1
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $923.30
$1319.00
OHu58380 XM_011531333.2
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu58381 XM_011531334.2
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
OHu22975 NM_001170961.1
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu58378 XM_011531330.1
Latest version!
Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu58380 XM_011531333.1 Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu58381 XM_011531334.1 Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu22594
    Clone ID Related Accession (Same CDS sequence) NM_001555.4
    Accession Version NM_001555.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-15
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590806.6 and BC063884.1. On Jan 7, 2010 this sequence version replaced NM_001555.3. Transcript Variant: This variant (1) uses an alternate in-frame splice site in a 3' exon compared to variant 3. The resulting protein (isoform 1) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063884.1, SRR1803615.4201.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGGAC TGTGCAATGG GGTATTGATA
    GAAGAGACTG AAATAGTCAT GCCAACCCCT AAGCCTGAGC TGTGGGCAGA GACCAACTTT
    CCTCTGGCCC CGTGGAAGAA CTTAACCCTC TGGTGCAGAA GCCCTTCTGG CTCAACTAAG
    GAGTTTGTGT TGCTGAAGGA TGGGACCGGG TGGATCGCCA CTCGCCCGGC CTCAGAGCAG
    GTCCGGGCTG CCTTCCCCCT TGGCGCCCTG ACCCAGAGCC ACACCGGGAG CTACCACTGC
    CATTCATGGG AGGAGATGGC TGTATCGGAG CCCAGTGAGG CACTTGAGCT GGTGGGGACA
    GACATCCTCC CCAAACCTGT CATTTCTGCT TCCCCCACAA TCCGGGGCCA GGAACTACAA
    CTCCGGTGCA AAGGATGGCT GGCAGGCATG GGGTTTGCTC TGTATAAGGA GGGAGAGCAA
    GAACCTGTCC AGCAACTTGG TGCTGTTGGA AGAGAAGCCT TCTTTACAAT CCAGAGAATG
    GAGGATAAAG ACGAAGGCAA TTACAGCTGC CGCACTCACA CTGAAAAACG CCCCTTCAAG
    TGGTCTGAGC CCAGTGAGCC GCTGGAGCTT GTCATAAAAG AAATGTACCC TAAGCCCTTC
    TTCAAGACAT GGGCCAGCCC TGTGGTCACC CCTGGTGCCC GAGTGACTTT CAATTGCTCC
    ACCCCCCACC AGCATATGAG CTTTATTCTT TACAAAGATG GAAGTGAAAT AGCATCCAGT
    GACAGGTCCT GGGCAAGTCC GGGGGCCAGT GCAGCTCACT TTCTAATCAT TTCGGTGGGC
    ATTGGTGATG GAGGGAATTA CAGCTGCCGA TATTATGACT TTTCTATCTG GTCTGAGCCC
    AGCGACCCTG TGGAGCTCGT GGTGACAGAA TTCTACCCCA AACCCACTCT CCTGGCACAG
    CCAGGTCCTG TGGTGTTTCC TGGGAAGAGT GTGATCCTGC GCTGCCAAGG GACTTTCCAG
    GGCATGAGGT TCGCCCTCTT GCAGGAGGGA GCCCATGTTC CCTTACAGTT TCGGAGTGTC
    TCAGGGAACT CAGCTGACTT CCTTCTCCAC ACTGTTGGAG CAGAGGACTC TGGGAACTAT
    AGCTGTATCT ACTATGAGAC AACCATGTCA AACAGGGGGT CATATCTCAG TATGCCCCTT
    ATGATCTGGG TGACTGACAC ATTCCCTAAG CCATGGTTGT TTGCTGAGCC CAGTTCTGTG
    GTTCCCATGG GGCAGAATGT TACTCTCTGG TGCCGAGGGC CGGTCCATGG AGTAGGATAC
    ATTCTGCACA AAGAAGGAGA AGCCACTTCA ATGCAGCTCT GGGGATCCAC CAGTAATGAC
    GGGGCATTCC CCATCACCAA TATATCTGGT ACTAGCATGG GGCGTTACAG CTGCTGCTAC
    CACCCTGACT GGACCAGTTC TATCAAGATA CAACCTAGCA ACACCCTGGA ACTCCTAGTC
    ACAGGCTTAC TCCCCAAACC CAGCCTATTA GCCCAGCCTG GTCCCATGGT GGCCCCTGGC
    GAAAATATGA CTCTTCAGTG TCAAGGGGAA CTGCCAGACT CAACATTTGT CCTGTTGAAG
    GAGGGGGCTC AGGAGCCTTT AGAGCAACAG AGGCCAAGTG GGTACAGGGC TGACTTCTGG
    ATGCCAGCAG TGAGAGGTGA AGACTCTGGG ATCTATAGCT GTGTTTATTA TTTGGACTCT
    ACTCCCTTTG CAGCTTCAAA TCACAGTGAC TCCCTGGAGA TCTGGGTGAC TGATAAGCCC
    CCTAAACCCT CTCTGTCAGC CTGGCCCAGC ACCATGTTCA AGTTAGGGAA GGACATCACC
    CTTCAGTGCC GAGGACCCCT GCCAGGTGTT GAATTTGTCC TAGAACATGA TGGAGAAGAA
    GCACCTCAGC AGTTTTCAGA GGATGGAGAC TTTGTCATCA ACAACGTAGA AGGAAAAGGC
    ATTGGAAACT ACAGCTGCAG CTACCGCCTC CAGGCCTACC CTGATATCTG GTCAGAGCCT
    AGTGATCCCC TGGAGCTGGT GGGGGCAGCA GGGCCTGTTG CTCAGGAGTG CACTGTAGGG
    AACATTGTCC GAAGTAGCCT AATCGTGGTG GTTGTTGTAG CCTTGGGGGT AGTGCTAGCC
    ATAGAGTGGA AGAAGTGGCC TCGACTGCGA ACCAGAGGCT CAGAGACAGA CGGAAGAGAC
    CAGACCATTG CCCTTGAAGA GTGTAACCAA GAAGGAGAAC CAGGCACCCC TGCCAATTCT
    CCTTCATCAA CCTCTCAGAG AATCTCTGTG GAACTGCCCG TTCCAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001546.2
    CDS284..4294
    Translation

    Target ORF information:

    RefSeq Version NM_001555.4
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001555.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGGAC TGTGCAATGG GGTATTGATA
    GAAGAGACTG AAATAGTCAT GCCAACCCCT AAGCCTGAGC TGTGGGCAGA GACCAACTTT
    CCTCTGGCCC CGTGGAAGAA CTTAACCCTC TGGTGCAGAA GCCCTTCTGG CTCAACTAAG
    GAGTTTGTGT TGCTGAAGGA TGGGACCGGG TGGATCGCCA CTCGCCCGGC CTCAGAGCAG
    GTCCGGGCTG CCTTCCCCCT TGGCGCCCTG ACCCAGAGCC ACACCGGGAG CTACCACTGC
    CATTCATGGG AGGAGATGGC TGTATCGGAG CCCAGTGAGG CACTTGAGCT GGTGGGGACA
    GACATCCTCC CCAAACCTGT CATTTCTGCT TCCCCCACAA TCCGGGGCCA GGAACTACAA
    CTCCGGTGCA AAGGATGGCT GGCAGGCATG GGGTTTGCTC TGTATAAGGA GGGAGAGCAA
    GAACCTGTCC AGCAACTTGG TGCTGTTGGA AGAGAAGCCT TCTTTACAAT CCAGAGAATG
    GAGGATAAAG ACGAAGGCAA TTACAGCTGC CGCACTCACA CTGAAAAACG CCCCTTCAAG
    TGGTCTGAGC CCAGTGAGCC GCTGGAGCTT GTCATAAAAG AAATGTACCC TAAGCCCTTC
    TTCAAGACAT GGGCCAGCCC TGTGGTCACC CCTGGTGCCC GAGTGACTTT CAATTGCTCC
    ACCCCCCACC AGCATATGAG CTTTATTCTT TACAAAGATG GAAGTGAAAT AGCATCCAGT
    GACAGGTCCT GGGCAAGTCC GGGGGCCAGT GCAGCTCACT TTCTAATCAT TTCGGTGGGC
    ATTGGTGATG GAGGGAATTA CAGCTGCCGA TATTATGACT TTTCTATCTG GTCTGAGCCC
    AGCGACCCTG TGGAGCTCGT GGTGACAGAA TTCTACCCCA AACCCACTCT CCTGGCACAG
    CCAGGTCCTG TGGTGTTTCC TGGGAAGAGT GTGATCCTGC GCTGCCAAGG GACTTTCCAG
    GGCATGAGGT TCGCCCTCTT GCAGGAGGGA GCCCATGTTC CCTTACAGTT TCGGAGTGTC
    TCAGGGAACT CAGCTGACTT CCTTCTCCAC ACTGTTGGAG CAGAGGACTC TGGGAACTAT
    AGCTGTATCT ACTATGAGAC AACCATGTCA AACAGGGGGT CATATCTCAG TATGCCCCTT
    ATGATCTGGG TGACTGACAC ATTCCCTAAG CCATGGTTGT TTGCTGAGCC CAGTTCTGTG
    GTTCCCATGG GGCAGAATGT TACTCTCTGG TGCCGAGGGC CGGTCCATGG AGTAGGATAC
    ATTCTGCACA AAGAAGGAGA AGCCACTTCA ATGCAGCTCT GGGGATCCAC CAGTAATGAC
    GGGGCATTCC CCATCACCAA TATATCTGGT ACTAGCATGG GGCGTTACAG CTGCTGCTAC
    CACCCTGACT GGACCAGTTC TATCAAGATA CAACCTAGCA ACACCCTGGA ACTCCTAGTC
    ACAGGCTTAC TCCCCAAACC CAGCCTATTA GCCCAGCCTG GTCCCATGGT GGCCCCTGGC
    GAAAATATGA CTCTTCAGTG TCAAGGGGAA CTGCCAGACT CAACATTTGT CCTGTTGAAG
    GAGGGGGCTC AGGAGCCTTT AGAGCAACAG AGGCCAAGTG GGTACAGGGC TGACTTCTGG
    ATGCCAGCAG TGAGAGGTGA AGACTCTGGG ATCTATAGCT GTGTTTATTA TTTGGACTCT
    ACTCCCTTTG CAGCTTCAAA TCACAGTGAC TCCCTGGAGA TCTGGGTGAC TGATAAGCCC
    CCTAAACCCT CTCTGTCAGC CTGGCCCAGC ACCATGTTCA AGTTAGGGAA GGACATCACC
    CTTCAGTGCC GAGGACCCCT GCCAGGTGTT GAATTTGTCC TAGAACATGA TGGAGAAGAA
    GCACCTCAGC AGTTTTCAGA GGATGGAGAC TTTGTCATCA ACAACGTAGA AGGAAAAGGC
    ATTGGAAACT ACAGCTGCAG CTACCGCCTC CAGGCCTACC CTGATATCTG GTCAGAGCCT
    AGTGATCCCC TGGAGCTGGT GGGGGCAGCA GGGCCTGTTG CTCAGGAGTG CACTGTAGGG
    AACATTGTCC GAAGTAGCCT AATCGTGGTG GTTGTTGTAG CCTTGGGGGT AGTGCTAGCC
    ATAGAGTGGA AGAAGTGGCC TCGACTGCGA ACCAGAGGCT CAGAGACAGA CGGAAGAGAC
    CAGACCATTG CCCTTGAAGA GTGTAACCAA GAAGGAGAAC CAGGCACCCC TGCCAATTCT
    CCTTCATCAA CCTCTCAGAG AATCTCTGTG GAACTGCCCG TTCCAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16613
    Clone ID Related Accession (Same CDS sequence) NM_205833.3 , NM_001170963.1
    Accession Version NM_205833.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590806.6 and AK057916.1. On Jan 7, 2010 this sequence version replaced NM_205833.2. Transcript Variant: This variant (2) differs in the 3' coding region and UTR compared to variant 3. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 3. Variants 2 and 5 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.159991.1, AB058894.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMN03267773 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGTG GGTGTGGCTA TGGCTGCTGG CATCTGGCAA TTGTTGTCCC AGGTATCATG
    GCTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_991402.1
    CDS284..1012
    Translation

    Target ORF information:

    RefSeq Version NM_205833.3
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_205833.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGTG GGTGTGGCTA TGGCTGCTGG CATCTGGCAA TTGTTGTCCC AGGTATCATG
    GCTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16613
    Clone ID Related Accession (Same CDS sequence) NM_205833.3 , NM_001170963.1
    Accession Version NM_001170963.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC028312.1. Transcript Variant: This variant (5) differs in the 5' UTR, and 3' coding region and UTR compared to variant 3. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 3. Variants 2 and 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028312.1, BI752398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120, SAMEA2148093 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGTG GGTGTGGCTA TGGCTGCTGG CATCTGGCAA TTGTTGTCCC AGGTATCATG
    GCTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001164434.1
    CDS108..836
    Translation

    Target ORF information:

    RefSeq Version NM_001170963.1
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170963.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGTG GGTGTGGCTA TGGCTGCTGG CATCTGGCAA TTGTTGTCCC AGGTATCATG
    GCTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108233
    Clone ID Related Accession (Same CDS sequence) NM_205833.4
    Accession Version NM_205833.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 729bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 2 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590806.6. On May 31, 2019 this sequence version replaced NM_205833.3. Transcript Variant: This variant (2) differs in the 3' coding region and UTR compared to variant 3. The resulting protein (isoform 2) has a distinct C-terminus and is shorter than isoform 3. Variants 2 and 5 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.159991.1, SRR5189667.323775.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2158188, SAMN03267773 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGTG GGTGTGGCTA TGGCTGCTGG CATCTGGCAA TTGTTGTCCC AGGTATCATG
    GCTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_991402.1
    CDS161..889
    Translation

    Target ORF information:

    RefSeq Version NM_205833.4
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_205833.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGTG GGTGTGGCTA TGGCTGCTGG CATCTGGCAA TTGTTGTCCC AGGTATCATG
    GCTGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107209
    Clone ID Related Accession (Same CDS sequence) NM_001555.5
    Accession Version NM_001555.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4011bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-29
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 1 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590806.6. On Nov 22, 2018 this sequence version replaced NM_001555.4. Transcript Variant: This variant (1) uses an alternate in-frame splice site in a 3' exon compared to variant 3. The resulting protein (isoform 1) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC063884.1, SRR1803615.4201.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361420.8/ ENSP00000355010.3 RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGGAC TGTGCAATGG GGTATTGATA
    GAAGAGACTG AAATAGTCAT GCCAACCCCT AAGCCTGAGC TGTGGGCAGA GACCAACTTT
    CCTCTGGCCC CGTGGAAGAA CTTAACCCTC TGGTGCAGAA GCCCTTCTGG CTCAACTAAG
    GAGTTTGTGT TGCTGAAGGA TGGGACCGGG TGGATCGCCA CTCGCCCGGC CTCAGAGCAG
    GTCCGGGCTG CCTTCCCCCT TGGCGCCCTG ACCCAGAGCC ACACCGGGAG CTACCACTGC
    CATTCATGGG AGGAGATGGC TGTATCGGAG CCCAGTGAGG CACTTGAGCT GGTGGGGACA
    GACATCCTCC CCAAACCTGT CATTTCTGCT TCCCCCACAA TCCGGGGCCA GGAACTACAA
    CTCCGGTGCA AAGGATGGCT GGCAGGCATG GGGTTTGCTC TGTATAAGGA GGGAGAGCAA
    GAACCTGTCC AGCAACTTGG TGCTGTTGGA AGAGAAGCCT TCTTTACAAT CCAGAGAATG
    GAGGATAAAG ACGAAGGCAA TTACAGCTGC CGCACTCACA CTGAAAAACG CCCCTTCAAG
    TGGTCTGAGC CCAGTGAGCC GCTGGAGCTT GTCATAAAAG AAATGTACCC TAAGCCCTTC
    TTCAAGACAT GGGCCAGCCC TGTGGTCACC CCTGGTGCCC GAGTGACTTT CAATTGCTCC
    ACCCCCCACC AGCATATGAG CTTTATTCTT TACAAAGATG GAAGTGAAAT AGCATCCAGT
    GACAGGTCCT GGGCAAGTCC GGGGGCCAGT GCAGCTCACT TTCTAATCAT TTCGGTGGGC
    ATTGGTGATG GAGGGAATTA CAGCTGCCGA TATTATGACT TTTCTATCTG GTCTGAGCCC
    AGCGACCCTG TGGAGCTCGT GGTGACAGAA TTCTACCCCA AACCCACTCT CCTGGCACAG
    CCAGGTCCTG TGGTGTTTCC TGGGAAGAGT GTGATCCTGC GCTGCCAAGG GACTTTCCAG
    GGCATGAGGT TCGCCCTCTT GCAGGAGGGA GCCCATGTTC CCTTACAGTT TCGGAGTGTC
    TCAGGGAACT CAGCTGACTT CCTTCTCCAC ACTGTTGGAG CAGAGGACTC TGGGAACTAT
    AGCTGTATCT ACTATGAGAC AACCATGTCA AACAGGGGGT CATATCTCAG TATGCCCCTT
    ATGATCTGGG TGACTGACAC ATTCCCTAAG CCATGGTTGT TTGCTGAGCC CAGTTCTGTG
    GTTCCCATGG GGCAGAATGT TACTCTCTGG TGCCGAGGGC CGGTCCATGG AGTAGGATAC
    ATTCTGCACA AAGAAGGAGA AGCCACTTCA ATGCAGCTCT GGGGATCCAC CAGTAATGAC
    GGGGCATTCC CCATCACCAA TATATCTGGT ACTAGCATGG GGCGTTACAG CTGCTGCTAC
    CACCCTGACT GGACCAGTTC TATCAAGATA CAACCTAGCA ACACCCTGGA ACTCCTAGTC
    ACAGGCTTAC TCCCCAAACC CAGCCTATTA GCCCAGCCTG GTCCCATGGT GGCCCCTGGC
    GAAAATATGA CTCTTCAGTG TCAAGGGGAA CTGCCAGACT CAACATTTGT CCTGTTGAAG
    GAGGGGGCTC AGGAGCCTTT AGAGCAACAG AGGCCAAGTG GGTACAGGGC TGACTTCTGG
    ATGCCAGCAG TGAGAGGTGA AGACTCTGGG ATCTATAGCT GTGTTTATTA TTTGGACTCT
    ACTCCCTTTG CAGCTTCAAA TCACAGTGAC TCCCTGGAGA TCTGGGTGAC TGATAAGCCC
    CCTAAACCCT CTCTGTCAGC CTGGCCCAGC ACCATGTTCA AGTTAGGGAA GGACATCACC
    CTTCAGTGCC GAGGACCCCT GCCAGGTGTT GAATTTGTCC TAGAACATGA TGGAGAAGAA
    GCACCTCAGC AGTTTTCAGA GGATGGAGAC TTTGTCATCA ACAACGTAGA AGGAAAAGGC
    ATTGGAAACT ACAGCTGCAG CTACCGCCTC CAGGCCTACC CTGATATCTG GTCAGAGCCT
    AGTGATCCCC TGGAGCTGGT GGGGGCAGCA GGGCCTGTTG CTCAGGAGTG CACTGTAGGG
    AACATTGTCC GAAGTAGCCT AATCGTGGTG GTTGTTGTAG CCTTGGGGGT AGTGCTAGCC
    ATAGAGTGGA AGAAGTGGCC TCGACTGCGA ACCAGAGGCT CAGAGACAGA CGGAAGAGAC
    CAGACCATTG CCCTTGAAGA GTGTAACCAA GAAGGAGAAC CAGGCACCCC TGCCAATTCT
    CCTTCATCAA CCTCTCAGAG AATCTCTGTG GAACTGCCCG TTCCAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001546.2
    CDS161..4171
    Translation

    Target ORF information:

    RefSeq Version NM_001555.5
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001555.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGGAC TGTGCAATGG GGTATTGATA
    GAAGAGACTG AAATAGTCAT GCCAACCCCT AAGCCTGAGC TGTGGGCAGA GACCAACTTT
    CCTCTGGCCC CGTGGAAGAA CTTAACCCTC TGGTGCAGAA GCCCTTCTGG CTCAACTAAG
    GAGTTTGTGT TGCTGAAGGA TGGGACCGGG TGGATCGCCA CTCGCCCGGC CTCAGAGCAG
    GTCCGGGCTG CCTTCCCCCT TGGCGCCCTG ACCCAGAGCC ACACCGGGAG CTACCACTGC
    CATTCATGGG AGGAGATGGC TGTATCGGAG CCCAGTGAGG CACTTGAGCT GGTGGGGACA
    GACATCCTCC CCAAACCTGT CATTTCTGCT TCCCCCACAA TCCGGGGCCA GGAACTACAA
    CTCCGGTGCA AAGGATGGCT GGCAGGCATG GGGTTTGCTC TGTATAAGGA GGGAGAGCAA
    GAACCTGTCC AGCAACTTGG TGCTGTTGGA AGAGAAGCCT TCTTTACAAT CCAGAGAATG
    GAGGATAAAG ACGAAGGCAA TTACAGCTGC CGCACTCACA CTGAAAAACG CCCCTTCAAG
    TGGTCTGAGC CCAGTGAGCC GCTGGAGCTT GTCATAAAAG AAATGTACCC TAAGCCCTTC
    TTCAAGACAT GGGCCAGCCC TGTGGTCACC CCTGGTGCCC GAGTGACTTT CAATTGCTCC
    ACCCCCCACC AGCATATGAG CTTTATTCTT TACAAAGATG GAAGTGAAAT AGCATCCAGT
    GACAGGTCCT GGGCAAGTCC GGGGGCCAGT GCAGCTCACT TTCTAATCAT TTCGGTGGGC
    ATTGGTGATG GAGGGAATTA CAGCTGCCGA TATTATGACT TTTCTATCTG GTCTGAGCCC
    AGCGACCCTG TGGAGCTCGT GGTGACAGAA TTCTACCCCA AACCCACTCT CCTGGCACAG
    CCAGGTCCTG TGGTGTTTCC TGGGAAGAGT GTGATCCTGC GCTGCCAAGG GACTTTCCAG
    GGCATGAGGT TCGCCCTCTT GCAGGAGGGA GCCCATGTTC CCTTACAGTT TCGGAGTGTC
    TCAGGGAACT CAGCTGACTT CCTTCTCCAC ACTGTTGGAG CAGAGGACTC TGGGAACTAT
    AGCTGTATCT ACTATGAGAC AACCATGTCA AACAGGGGGT CATATCTCAG TATGCCCCTT
    ATGATCTGGG TGACTGACAC ATTCCCTAAG CCATGGTTGT TTGCTGAGCC CAGTTCTGTG
    GTTCCCATGG GGCAGAATGT TACTCTCTGG TGCCGAGGGC CGGTCCATGG AGTAGGATAC
    ATTCTGCACA AAGAAGGAGA AGCCACTTCA ATGCAGCTCT GGGGATCCAC CAGTAATGAC
    GGGGCATTCC CCATCACCAA TATATCTGGT ACTAGCATGG GGCGTTACAG CTGCTGCTAC
    CACCCTGACT GGACCAGTTC TATCAAGATA CAACCTAGCA ACACCCTGGA ACTCCTAGTC
    ACAGGCTTAC TCCCCAAACC CAGCCTATTA GCCCAGCCTG GTCCCATGGT GGCCCCTGGC
    GAAAATATGA CTCTTCAGTG TCAAGGGGAA CTGCCAGACT CAACATTTGT CCTGTTGAAG
    GAGGGGGCTC AGGAGCCTTT AGAGCAACAG AGGCCAAGTG GGTACAGGGC TGACTTCTGG
    ATGCCAGCAG TGAGAGGTGA AGACTCTGGG ATCTATAGCT GTGTTTATTA TTTGGACTCT
    ACTCCCTTTG CAGCTTCAAA TCACAGTGAC TCCCTGGAGA TCTGGGTGAC TGATAAGCCC
    CCTAAACCCT CTCTGTCAGC CTGGCCCAGC ACCATGTTCA AGTTAGGGAA GGACATCACC
    CTTCAGTGCC GAGGACCCCT GCCAGGTGTT GAATTTGTCC TAGAACATGA TGGAGAAGAA
    GCACCTCAGC AGTTTTCAGA GGATGGAGAC TTTGTCATCA ACAACGTAGA AGGAAAAGGC
    ATTGGAAACT ACAGCTGCAG CTACCGCCTC CAGGCCTACC CTGATATCTG GTCAGAGCCT
    AGTGATCCCC TGGAGCTGGT GGGGGCAGCA GGGCCTGTTG CTCAGGAGTG CACTGTAGGG
    AACATTGTCC GAAGTAGCCT AATCGTGGTG GTTGTTGTAG CCTTGGGGGT AGTGCTAGCC
    ATAGAGTGGA AGAAGTGGCC TCGACTGCGA ACCAGAGGCT CAGAGACAGA CGGAAGAGAC
    CAGACCATTG CCCTTGAAGA GTGTAACCAA GAAGGAGAAC CAGGCACCCC TGCCAATTCT
    CCTTCATCAA CCTCTCAGAG AATCTCTGTG GAACTGCCCG TTCCAATATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu108258
    Clone ID Related Accession (Same CDS sequence) NM_001170962.2
    Accession Version NM_001170962.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590806.6. On Jun 1, 2019 this sequence version replaced NM_001170962.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in a 3' exon compared to variant 3. The resulting protein (isoform 4) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF034198.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC TGATGGACCC TCAACCAGAG TTGTGGATAG AGTCCAACTA CCCCCAGGCC
    CCTTGGGAGA ACATCACGCT TTGGTGCCGA AGCCCCTCTC GGATATCAAG CAAGTTCCTG
    CTGCTGAAGG ATAAGACACA GATGACCTGG ATCCGCCCTT CCCACAAGAC CTTCCAAGTT
    TCATTCCTTA TAGGTGCCCT TACTGAGTCC AATGCAGGTC TTTACCGGTG CTGCTACTGG
    AAGGAGACAG GCTGGTCAAA GCCCAGTAAA GTTCTAGAGT TGGAGGCACC AGGCCAACTG
    CCCAAGCCCA TCTTCTGGAT TCAGGCTGAG ACCCCCGCTC TTCCTGGGTG TAATGTTAAC
    ATCCTCTGCC ATGGCTGGCT GCAGGATTTG GTATTCATGC TGTTTAAAGA GGGATATGCA
    GAGCCTGTGG ATTACCAAGT CCCAACTGGG ACAATGGCCA TATTCTCCAT TGACAACCTG
    ACACCTGAGG ATGAAGGGGT TTACATCTGC CGCACTCATA TCCAGATGCT CCCCACCCTG
    TGGTCAGAGC CCAGCAACCC CCTGAAGCTG GTTGTAGCAG GACTCTACCC CAAACCAACT
    TTGACAGCCC ATCCTGGGCC CATCATGGCA CCTGGAGAAA GCCTGAATCT CAGGTGCCAA
    GGGCCAATCT ATGGAATGAC CTTTGCTCTA ATGAGGGTTG AAGACTTGGA GAAGTCCTTT
    TACCACAAGA AGACAATAAA AAATGAGGCA AATTTCTTCT TCCAGTCTTT GAAGATCCAA
    GATACTGGAC ATTACCTCTG TTTTTACTAT GACGCATCAT ATAGAGGTTC ACTCCTTAGT
    GATGTCCTGA AAATCTGGGT GACTGACACT TTCCCCAAGA CCTGGCTACT TGCTCGGCCC
    AGTGCTGTGG TCCAAATGGG TCAGAATGTG AGCCTACGGT GTCGAGGACC AGTGGATGGA
    GTGGGTCTTG CACTCTATAA GAAAGGAGAA GACAAACCAC TTCAATTTTT GGATGCCACC
    AGCATCGATG ACAACACATC ATTCTTCCTC AACAATGTAA CCTACAGTGA TACTGGCATC
    TATAGCTGCC ACTATCTTCT CACCTGGAAG ACCTCCATTA GGATGCCATC ACACAACACT
    GTGGAGCTTA TGGTTGTAGA TAAGCCCCCC AAACCCTCCC TGTCAGCTTG GCCAAGCACT
    GTGTTCAAGC TAGGAAAGGC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTACTGGAA
    TTTTCTCTGG AATGGGAAGA AAGAGAAACA TTCCAAAAAT TCTCAGTAAA CGGAGACTTC
    ATCATCAGTA ATGTTGACGG GAAAGGCACA GGGACCTACA GTTGCAGCTA TCGCGTAGAG
    ACACATCCTA ACATCTGGTC ACATCGCAGT GAGCCCCTGA AGCTGATGGG GCCAGCAGGC
    TATCTCACCT GGAATTACGT TCTGAATGAA GCTATCAGGT TGTCTCTAAT CATGCAGCTT
    GTTGCCTTGC TGTTGGTAGT GCTGTGGATA AGGTGGAAGT GTCGGAGACT CAGAATCAGA
    GAAGCCTGGT TGCTGGGAAC AGCTCAAGGG GTCACCATGC TCTTCATAGT CACGGCCCTT
    CTCTGCTGTG GACTGTGCAA TGGGGTATTG ATAGAAGAGA CTGAAATAGT CATGCCAACC
    CCTAAGCCTG AGCTGTGGGC AGAGACCAAC TTTCCTCTGG CCCCGTGGAA GAACTTAACC
    CTCTGGTGCA GAAGCCCTTC TGGCTCAACT AAGGAGTTTG TGTTGCTGAA GGATGGGACC
    GGGTGGATCG CCACTCGCCC GGCCTCAGAG CAGGTCCGGG CTGCCTTCCC CCTTGGCGCC
    CTGACCCAGA GCCACACCGG GAGCTACCAC TGCCATTCAT GGGAGGAGAT GGCTGTATCG
    GAGCCCAGTG AGGCACTTGA GCTGGTGGGG ACAGACATCC TCCCCAAACC TGTCATTTCT
    GCTTCCCCCA CAATCCGGGG CCAGGAACTA CAACTCCGGT GCAAAGGATG GCTGGCAGGC
    ATGGGGTTTG CTCTGTATAA GGAGGGAGAG CAAGAACCTG TCCAGCAACT TGGTGCTGTT
    GGAAGAGAAG CCTTCTTTAC AATCCAGAGA ATGGAGGATA AAGACGAAGG CAATTACAGC
    TGCCGCACTC ACACTGAAAA ACGCCCCTTC AAGTGGTCTG AGCCCAGTGA GCCGCTGGAG
    CTTGTCATAA AAGAAATGTA CCCTAAGCCC TTCTTCAAGA CATGGGCCAG CCCTGTGGTC
    ACCCCTGGTG CCCGAGTGAC TTTCAATTGC TCCACCCCCC ACCAGCATAT GAGCTTTATT
    CTTTACAAAG ATGGAAGTGA AATAGCATCC AGTGACAGGT CCTGGGCAAG TCCGGGGGCC
    AGTGCAGCTC ACTTTCTAAT CATTTCGGTG GGCATTGGTG ATGGAGGGAA TTACAGCTGC
    CGATATTATG ACTTTTCTAT CTGGTCTGAG CCCAGCGACC CTGTGGAGCT CGTGGTGACA
    GAATTCTACC CCAAACCCAC TCTCCTGGCA CAGCCAGGTC CTGTGGTGTT TCCTGGGAAG
    AGTGTGATCC TGCGCTGCCA AGGGACTTTC CAGGGCATGA GGTTCGCCCT CTTGCAGGAG
    GGAGCCCATG TTCCCTTACA GTTTCGGAGT GTCTCAGGGA ACTCAGCTGA CTTCCTTCTC
    CACACTGTTG GAGCAGAGGA CTCTGGGAAC TATAGCTGTA TCTACTATGA GACAACCATG
    TCAAACAGGG GGTCATATCT CAGTATGCCC CTTATGATCT GGGTGACTGA CACATTCCCT
    AAGCCATGGT TGTTTGCTGA GCCCAGTTCT GTGGTTCCCA TGGGGCAGAA TGTTACTCTC
    TGGTGCCGAG GGCCGGTCCA TGGAGTAGGA TACATTCTGC ACAAAGAAGG AGAAGCCACT
    TCAATGCAGC TCTGGGGATC CACCAGTAAT GACGGGGCAT TCCCCATCAC CAATATATCT
    GGTACTAGCA TGGGGCGTTA CAGCTGCTGC TACCACCCTG ACTGGACCAG TTCTATCAAG
    ATACAACCTA GCAACACCCT GGAACTCCTA GTCACAGGCT TACTCCCCAA ACCCAGCCTA
    TTAGCCCAGC CTGGTCCCAT GGTGGCCCCT GGCGAAAATA TGACTCTTCA GTGTCAAGGG
    GAACTGCCAG ACTCAACATT TGTCCTGTTG AAGGAGGGGG CTCAGGAGCC TTTAGAGCAA
    CAGAGGCCAA GTGGGTACAG GGCTGACTTC TGGATGCCAG CAGTGAGAGG TGAAGACTCT
    GGGATCTATA GCTGTGTTTA TTATTTGGAC TCTACTCCCT TTGCAGCTTC AAATCACAGT
    GACTCCCTGG AGATCTGGGT GACTGATAAG CCCCCTAAAC CCTCTCTGTC AGCCTGGCCC
    AGCACCATGT TCAAGTTAGG GAAGGACATC ACCCTTCAGT GCCGAGGACC CCTGCCAGGT
    GTTGAATTTG TCCTAGAACA TGATGGAGAA GAAGCACCTC AGCAGTTTTC AGAGGATGGA
    GACTTTGTCA TCAACAACGT AGAAGGAAAA GGCATTGGAA ACTACAGCTG CAGCTACCGC
    CTCCAGGCCT ACCCTGATAT CTGGTCAGAG CCTAGTGATC CCCTGGAGCT GGTGGGGGCA
    GCAGGGCCTG TTGCTCAGGA GTGCACTGTA GGGAACATTG TCCGAAGTAG CCTAATCGTG
    GTGGTTGTTG TAGCCTTGGG GGTAGTGCTA GCCATAGAGT GGAAGAAGTG GCCTCGACTG
    CGAACCAGAG GCTCAGAGAC AGACGGAAGA GACCAGACCA TTGCCCTTGA AGAGTGTAAC
    CAAGAAGGAG AACCAGGCAC CCCTGCCAAT TCTCCTTCAT CAACCTCTCA GAGAATCTCT
    GTGGAACTGC CCGTTCCAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001164433.1
    CDS161..4144
    Translation

    Target ORF information:

    RefSeq Version NM_001170962.2
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170962.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC TGATGGACCC TCAACCAGAG TTGTGGATAG AGTCCAACTA CCCCCAGGCC
    CCTTGGGAGA ACATCACGCT TTGGTGCCGA AGCCCCTCTC GGATATCAAG CAAGTTCCTG
    CTGCTGAAGG ATAAGACACA GATGACCTGG ATCCGCCCTT CCCACAAGAC CTTCCAAGTT
    TCATTCCTTA TAGGTGCCCT TACTGAGTCC AATGCAGGTC TTTACCGGTG CTGCTACTGG
    AAGGAGACAG GCTGGTCAAA GCCCAGTAAA GTTCTAGAGT TGGAGGCACC AGGCCAACTG
    CCCAAGCCCA TCTTCTGGAT TCAGGCTGAG ACCCCCGCTC TTCCTGGGTG TAATGTTAAC
    ATCCTCTGCC ATGGCTGGCT GCAGGATTTG GTATTCATGC TGTTTAAAGA GGGATATGCA
    GAGCCTGTGG ATTACCAAGT CCCAACTGGG ACAATGGCCA TATTCTCCAT TGACAACCTG
    ACACCTGAGG ATGAAGGGGT TTACATCTGC CGCACTCATA TCCAGATGCT CCCCACCCTG
    TGGTCAGAGC CCAGCAACCC CCTGAAGCTG GTTGTAGCAG GACTCTACCC CAAACCAACT
    TTGACAGCCC ATCCTGGGCC CATCATGGCA CCTGGAGAAA GCCTGAATCT CAGGTGCCAA
    GGGCCAATCT ATGGAATGAC CTTTGCTCTA ATGAGGGTTG AAGACTTGGA GAAGTCCTTT
    TACCACAAGA AGACAATAAA AAATGAGGCA AATTTCTTCT TCCAGTCTTT GAAGATCCAA
    GATACTGGAC ATTACCTCTG TTTTTACTAT GACGCATCAT ATAGAGGTTC ACTCCTTAGT
    GATGTCCTGA AAATCTGGGT GACTGACACT TTCCCCAAGA CCTGGCTACT TGCTCGGCCC
    AGTGCTGTGG TCCAAATGGG TCAGAATGTG AGCCTACGGT GTCGAGGACC AGTGGATGGA
    GTGGGTCTTG CACTCTATAA GAAAGGAGAA GACAAACCAC TTCAATTTTT GGATGCCACC
    AGCATCGATG ACAACACATC ATTCTTCCTC AACAATGTAA CCTACAGTGA TACTGGCATC
    TATAGCTGCC ACTATCTTCT CACCTGGAAG ACCTCCATTA GGATGCCATC ACACAACACT
    GTGGAGCTTA TGGTTGTAGA TAAGCCCCCC AAACCCTCCC TGTCAGCTTG GCCAAGCACT
    GTGTTCAAGC TAGGAAAGGC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTACTGGAA
    TTTTCTCTGG AATGGGAAGA AAGAGAAACA TTCCAAAAAT TCTCAGTAAA CGGAGACTTC
    ATCATCAGTA ATGTTGACGG GAAAGGCACA GGGACCTACA GTTGCAGCTA TCGCGTAGAG
    ACACATCCTA ACATCTGGTC ACATCGCAGT GAGCCCCTGA AGCTGATGGG GCCAGCAGGC
    TATCTCACCT GGAATTACGT TCTGAATGAA GCTATCAGGT TGTCTCTAAT CATGCAGCTT
    GTTGCCTTGC TGTTGGTAGT GCTGTGGATA AGGTGGAAGT GTCGGAGACT CAGAATCAGA
    GAAGCCTGGT TGCTGGGAAC AGCTCAAGGG GTCACCATGC TCTTCATAGT CACGGCCCTT
    CTCTGCTGTG GACTGTGCAA TGGGGTATTG ATAGAAGAGA CTGAAATAGT CATGCCAACC
    CCTAAGCCTG AGCTGTGGGC AGAGACCAAC TTTCCTCTGG CCCCGTGGAA GAACTTAACC
    CTCTGGTGCA GAAGCCCTTC TGGCTCAACT AAGGAGTTTG TGTTGCTGAA GGATGGGACC
    GGGTGGATCG CCACTCGCCC GGCCTCAGAG CAGGTCCGGG CTGCCTTCCC CCTTGGCGCC
    CTGACCCAGA GCCACACCGG GAGCTACCAC TGCCATTCAT GGGAGGAGAT GGCTGTATCG
    GAGCCCAGTG AGGCACTTGA GCTGGTGGGG ACAGACATCC TCCCCAAACC TGTCATTTCT
    GCTTCCCCCA CAATCCGGGG CCAGGAACTA CAACTCCGGT GCAAAGGATG GCTGGCAGGC
    ATGGGGTTTG CTCTGTATAA GGAGGGAGAG CAAGAACCTG TCCAGCAACT TGGTGCTGTT
    GGAAGAGAAG CCTTCTTTAC AATCCAGAGA ATGGAGGATA AAGACGAAGG CAATTACAGC
    TGCCGCACTC ACACTGAAAA ACGCCCCTTC AAGTGGTCTG AGCCCAGTGA GCCGCTGGAG
    CTTGTCATAA AAGAAATGTA CCCTAAGCCC TTCTTCAAGA CATGGGCCAG CCCTGTGGTC
    ACCCCTGGTG CCCGAGTGAC TTTCAATTGC TCCACCCCCC ACCAGCATAT GAGCTTTATT
    CTTTACAAAG ATGGAAGTGA AATAGCATCC AGTGACAGGT CCTGGGCAAG TCCGGGGGCC
    AGTGCAGCTC ACTTTCTAAT CATTTCGGTG GGCATTGGTG ATGGAGGGAA TTACAGCTGC
    CGATATTATG ACTTTTCTAT CTGGTCTGAG CCCAGCGACC CTGTGGAGCT CGTGGTGACA
    GAATTCTACC CCAAACCCAC TCTCCTGGCA CAGCCAGGTC CTGTGGTGTT TCCTGGGAAG
    AGTGTGATCC TGCGCTGCCA AGGGACTTTC CAGGGCATGA GGTTCGCCCT CTTGCAGGAG
    GGAGCCCATG TTCCCTTACA GTTTCGGAGT GTCTCAGGGA ACTCAGCTGA CTTCCTTCTC
    CACACTGTTG GAGCAGAGGA CTCTGGGAAC TATAGCTGTA TCTACTATGA GACAACCATG
    TCAAACAGGG GGTCATATCT CAGTATGCCC CTTATGATCT GGGTGACTGA CACATTCCCT
    AAGCCATGGT TGTTTGCTGA GCCCAGTTCT GTGGTTCCCA TGGGGCAGAA TGTTACTCTC
    TGGTGCCGAG GGCCGGTCCA TGGAGTAGGA TACATTCTGC ACAAAGAAGG AGAAGCCACT
    TCAATGCAGC TCTGGGGATC CACCAGTAAT GACGGGGCAT TCCCCATCAC CAATATATCT
    GGTACTAGCA TGGGGCGTTA CAGCTGCTGC TACCACCCTG ACTGGACCAG TTCTATCAAG
    ATACAACCTA GCAACACCCT GGAACTCCTA GTCACAGGCT TACTCCCCAA ACCCAGCCTA
    TTAGCCCAGC CTGGTCCCAT GGTGGCCCCT GGCGAAAATA TGACTCTTCA GTGTCAAGGG
    GAACTGCCAG ACTCAACATT TGTCCTGTTG AAGGAGGGGG CTCAGGAGCC TTTAGAGCAA
    CAGAGGCCAA GTGGGTACAG GGCTGACTTC TGGATGCCAG CAGTGAGAGG TGAAGACTCT
    GGGATCTATA GCTGTGTTTA TTATTTGGAC TCTACTCCCT TTGCAGCTTC AAATCACAGT
    GACTCCCTGG AGATCTGGGT GACTGATAAG CCCCCTAAAC CCTCTCTGTC AGCCTGGCCC
    AGCACCATGT TCAAGTTAGG GAAGGACATC ACCCTTCAGT GCCGAGGACC CCTGCCAGGT
    GTTGAATTTG TCCTAGAACA TGATGGAGAA GAAGCACCTC AGCAGTTTTC AGAGGATGGA
    GACTTTGTCA TCAACAACGT AGAAGGAAAA GGCATTGGAA ACTACAGCTG CAGCTACCGC
    CTCCAGGCCT ACCCTGATAT CTGGTCAGAG CCTAGTGATC CCCTGGAGCT GGTGGGGGCA
    GCAGGGCCTG TTGCTCAGGA GTGCACTGTA GGGAACATTG TCCGAAGTAG CCTAATCGTG
    GTGGTTGTTG TAGCCTTGGG GGTAGTGCTA GCCATAGAGT GGAAGAAGTG GCCTCGACTG
    CGAACCAGAG GCTCAGAGAC AGACGGAAGA GACCAGACCA TTGCCCTTGA AGAGTGTAAC
    CAAGAAGGAG AACCAGGCAC CCCTGCCAAT TCTCCTTCAT CAACCTCTCA GAGAATCTCT
    GTGGAACTGC CCGTTCCAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22998
    Clone ID Related Accession (Same CDS sequence) NM_001170962.1
    Accession Version NM_001170962.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3984bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590806.6 and AF034198.1. Transcript Variant: This variant (4) lacks an alternate in-frame exon in the 5' coding region and uses an alternate in-frame splice site in a 3' exon compared to variant 3. The resulting protein (isoform 4) is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF034198.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC TGATGGACCC TCAACCGGAG TTGTGGATAG AGTCCAACTA CCCCCAGGCC
    CCTTGGGAGA ACATCACGCT TTGGTGCCGA AGCCCCTCTC GGATATCAAG CAAGTTCCTG
    CTGCTGAAGG ATAAGACACA GATGACCTGG ATCCGCCCTT CCCACAAGAC CTTCCAAGTT
    TCATTCCTTA TAGGTGCCCT TACTGAGTCC AATGCAGGTC TTTACCGGTG CTGCTACTGG
    AAGGAGACAG GCTGGTCAAA GCCCAGTAAA GTTCTAGAGT TGGAGGCACC AGGCCAACTG
    CCCAAGCCCA TCTTCTGGAT TCAGGCTGAG ACCCCCGCTC TTCCTGGGTG TAATGTTAAC
    ATCCTCTGCC ATGGCTGGCT GCAGGATTTG GTATTCATGC TGTTTAAAGA GGGATATGCA
    GAGCCTGTGG ATTACCAAGT CCCAACTGGG ACAATGGCCA TATTCTCCAT TGACAACCTG
    ACACCTGAGG ATGAAGGGGT TTACATCTGC CGCACTCATA TCCAGATGCT CCCCACCCTG
    TGGTCAGAGC CCAGCAACCC CCTGAAGCTG GTTGTAGCAG GACTCTACCC CAAACCAACT
    TTGACAGCCC ATCCTGGGCC CATCATGGCA CCTGGAGAAA GCCTGAATCT CAGGTGCCAA
    GGGCCAATCT ATGGAATGAC CTTTGCTCTA ATGAGGGTTG AAGACTTGGA GAAGTCCTTT
    TACCACAAGA AGACAATAAA AAATGAGGCA AATTTCTTCT TCCAGTCTTT GAAGATCCAA
    GATACTGGAC ATTACCTCTG TTTTTACTAT GACGCATCAT ATAGAGGTTC ACTCCTTAGT
    GATGTCCTGA AAATCTGGGT GACTGACACT TTCCCCAAGA CCTGGCTACT TGCTCGGCCC
    AGTGCTGTGG TCCAAATGGG TCAGAATGTG AGCCTACGGT GTCGAGGACC AGTGGATGGA
    GTGGGTCTTG CACTCTATAA GAAAGGAGAA GACAAACCAC TTCAATTTTT GGATGCCACC
    AGCATCGATG ACAACACATC ATTCTTCCTC AACAATGTAA CCTACAGTGA TACTGGCATC
    TATAGCTGCC ACTATCTTCT CACCTGGAAG ACCTCCATTA GGATGCCATC ACACAACACT
    GTGGAGCTTA TGGTTGTAGA TAAGCCCCCC AAACCCTCCC TGTCAGCTTG GCCAAGCACT
    GTGTTCAAGC TAGGAAAGGC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTACTGGAA
    TTTTCTCTGG AATGGGAAGA AAGAGAAACA TTCCAAAAAT TCTCAGTAAA CGGAGACTTC
    ATCATCAGTA ATGTTGACGG GAAAGGCACA GGGACCTACA GTTGCAGCTA TCGCGTAGAG
    ACACATCCTA ACATCTGGTC ACATCGCAGT GAGCCCCTGA AGCTGATGGG GCCAGCAGGC
    TATCTCACCT GGAATTACGT TCTGAATGAA GCTATCAGGT TGTCTCTAAT CATGCAGCTT
    GTTGCCTTGC TGTTGGTAGT GCTGTGGATA AGGTGGAAGT GTCGGAGACT CAGAATCAGA
    GAAGCCTGGT TGCTGGGAAC AGCTCAAGGG GTCACCATGC TCTTCATAGT CACGGCCCTT
    CTCTGCTGTG GACTGTGCAA TGGGGTATTG ATAGAAGAGA CTGAAATAGT CATGCCAACC
    CCTAAGCCTG AGCTGTGGGC AGAGACCAAC TTTCCTCTGG CCCCGTGGAA GAACTTAACC
    CTCTGGTGCA GAAGCCCTTC TGGCTCAACT AAGGAGTTTG TGTTGCTGAA GGATGGGACC
    GGGTGGATCG CCACTCGCCC GGCCTCAGAG CAGGTCCGGG CTGCCTTCCC CCTTGGCGCC
    CTGACCCAGA GCCACACCGG GAGCTACCAC TGCCATTCAT GGGAGGAGAT GGCTGTATCG
    GAGCCCAGTG AGGCACTTGA GCTGGTGGGG ACAGACATCC TCCCCAAACC TGTCATTTCT
    GCTTCCCCCA CAATCCGGGG CCAGGAACTA CAACTCCGGT GCAAAGGATG GCTGGCAGGC
    ATGGGGTTTG CTCTGTATAA GGAGGGAGAG CAAGAACCTG TCCAGCAACT TGGTGCTGTT
    GGAAGAGAAG CCTTCTTTAC AATCCAGAGA ATGGAGGATA AAGACGAAGG CAATTACAGC
    TGCCGCACTC ACACTGAAAA ACGCCCCTTC AAGTGGTCTG AGCCCAGTGA GCCGCTGGAG
    CTTGTCATAA AAGAAATGTA CCCTAAGCCC TTCTTCAAGA CATGGGCCAG CCCTGTGGTC
    ACCCCTGGTG CCCGAGTGAC TTTCAATTGC TCCACCCCCC ACCAGCATAT GAGCTTTATT
    CTTTACAAAG ATGGAAGTGA AATAGCATCC AGTGACAGGT CCTGGGCAAG TCCGGGGGCC
    AGTGCAGCTC ACTTTCTAAT CATTTCGGTG GGCATTGGTG ATGGAGGGAA TTACAGCTGC
    CGATATTATG ACTTTTCTAT CTGGTCTGAG CCCAGCGACC CTGTGGAGCT CGTGGTGACA
    GAATTCTACC CCAAACCCAC TCTCCTGGCA CAGCCAGGTC CTGTGGTGTT TCCTGGGAAG
    AGTGTGATCC TGCGCTGCCA AGGGACTTTC CAGGGCATGA GGTTCGCCCT CTTGCAGGAG
    GGAGCCCATG TTCCCTTACA GTTTCGGAGT GTCTCAGGGA ACTCAGCTGA CTTCCTTCTC
    CACACTGTTG GAGCAGAGGA CTCTGGGAAC TATAGCTGTA TCTACTATGA GACAACCATG
    TCAAACAGGG GGTCATATCT CAGTATGCCC CTTATGATCT GGGTGACTGA CACATTCCCT
    AAGCCATGGT TGTTTGCTGA GCCCAGTTCT GTGGTTCCCA TGGGGCAGAA TGTTACTCTC
    TGGTGCCGAG GGCCGGTCCA TGGAGTAGGA TACATTCTGC ACAAAGAAGG AGAAGCCACT
    TCAATGCAGC TCTGGGGATC CACCAGTAAT GACGGGGCAT TCCCCATCAC CAATATATCT
    GGTACTAGCA TGGGGCGTTA CAGCTGCTGC TACCACCCTG ACTGGACCAG TTCTATCAAG
    ATACAACCTA GCAACACCCT GGAACTCCTA GTCACAGGCT TACTCCCCAA ACCCAGCCTA
    TTAGCCCAGC CTGGTCCCAT GGTGGCCCCT GGCGAAAATA TGACTCTTCA GTGTCAAGGG
    GAACTGCCAG ACTCAACATT TGTCCTGTTG AAGGAGGGGG CTCAGGAGCC TTTAGAGCAA
    CAGAGGCCAA GTGGGTACAG GGCTGACTTC TGGATGCCAG CAGTGAGAGG TGAAGACTCT
    GGGATCTATA GCTGTGTTTA TTATTTGGAC TCTACTCCCT TTGCAGCTTC AAATCACAGT
    GACTCCCTGG AGATCTGGGT GACTGATAAG CCCCCTAAAC CCTCTCTGTC AGCCTGGCCC
    AGCACCATGT TCAAGTTAGG GAAGGACATC ACCCTTCAGT GCCGAGGACC CCTGCCAGGT
    GTTGAATTTG TCCTAGAACA TGATGGAGAA GAAGCACCTC AGCAGTTTTC AGAGGATGGA
    GACTTTGTCA TCAACAACGT AGAAGGAAAA GGCATTGGAA ACTACAGCTG CAGCTACCGC
    CTCCAGGCCT ACCCTGATAT CTGGTCAGAG CCTAGTGATC CCCTGGAGCT GGTGGGGGCA
    GCAGGGCCTG TTGCTCAGGA GTGCACTGTA GGGAACATTG TCCGAAGTAG CCTAATCGTG
    GTGGTTGTTG TAGCCTTGGG GGTAGTGCTA GCCATAGAGT GGAAGAAGTG GCCTCGACTG
    CGAACCAGAG GCTCAGAGAC AGACGGAAGA GACCAGACCA TTGCCCTTGA AGAGTGTAAC
    CAAGAAGGAG AACCAGGCAC CCCTGCCAAT TCTCCTTCAT CAACCTCTCA GAGAATCTCT
    GTGGAACTGC CCGTTCCAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001164433.1
    CDS284..4267
    Translation

    Target ORF information:

    RefSeq Version NM_001170962.1
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170962.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC TGATGGACCC TCAACCGGAG TTGTGGATAG AGTCCAACTA CCCCCAGGCC
    CCTTGGGAGA ACATCACGCT TTGGTGCCGA AGCCCCTCTC GGATATCAAG CAAGTTCCTG
    CTGCTGAAGG ATAAGACACA GATGACCTGG ATCCGCCCTT CCCACAAGAC CTTCCAAGTT
    TCATTCCTTA TAGGTGCCCT TACTGAGTCC AATGCAGGTC TTTACCGGTG CTGCTACTGG
    AAGGAGACAG GCTGGTCAAA GCCCAGTAAA GTTCTAGAGT TGGAGGCACC AGGCCAACTG
    CCCAAGCCCA TCTTCTGGAT TCAGGCTGAG ACCCCCGCTC TTCCTGGGTG TAATGTTAAC
    ATCCTCTGCC ATGGCTGGCT GCAGGATTTG GTATTCATGC TGTTTAAAGA GGGATATGCA
    GAGCCTGTGG ATTACCAAGT CCCAACTGGG ACAATGGCCA TATTCTCCAT TGACAACCTG
    ACACCTGAGG ATGAAGGGGT TTACATCTGC CGCACTCATA TCCAGATGCT CCCCACCCTG
    TGGTCAGAGC CCAGCAACCC CCTGAAGCTG GTTGTAGCAG GACTCTACCC CAAACCAACT
    TTGACAGCCC ATCCTGGGCC CATCATGGCA CCTGGAGAAA GCCTGAATCT CAGGTGCCAA
    GGGCCAATCT ATGGAATGAC CTTTGCTCTA ATGAGGGTTG AAGACTTGGA GAAGTCCTTT
    TACCACAAGA AGACAATAAA AAATGAGGCA AATTTCTTCT TCCAGTCTTT GAAGATCCAA
    GATACTGGAC ATTACCTCTG TTTTTACTAT GACGCATCAT ATAGAGGTTC ACTCCTTAGT
    GATGTCCTGA AAATCTGGGT GACTGACACT TTCCCCAAGA CCTGGCTACT TGCTCGGCCC
    AGTGCTGTGG TCCAAATGGG TCAGAATGTG AGCCTACGGT GTCGAGGACC AGTGGATGGA
    GTGGGTCTTG CACTCTATAA GAAAGGAGAA GACAAACCAC TTCAATTTTT GGATGCCACC
    AGCATCGATG ACAACACATC ATTCTTCCTC AACAATGTAA CCTACAGTGA TACTGGCATC
    TATAGCTGCC ACTATCTTCT CACCTGGAAG ACCTCCATTA GGATGCCATC ACACAACACT
    GTGGAGCTTA TGGTTGTAGA TAAGCCCCCC AAACCCTCCC TGTCAGCTTG GCCAAGCACT
    GTGTTCAAGC TAGGAAAGGC CATCACCCTT CAGTGCCGAG TATCTCATCC AGTACTGGAA
    TTTTCTCTGG AATGGGAAGA AAGAGAAACA TTCCAAAAAT TCTCAGTAAA CGGAGACTTC
    ATCATCAGTA ATGTTGACGG GAAAGGCACA GGGACCTACA GTTGCAGCTA TCGCGTAGAG
    ACACATCCTA ACATCTGGTC ACATCGCAGT GAGCCCCTGA AGCTGATGGG GCCAGCAGGC
    TATCTCACCT GGAATTACGT TCTGAATGAA GCTATCAGGT TGTCTCTAAT CATGCAGCTT
    GTTGCCTTGC TGTTGGTAGT GCTGTGGATA AGGTGGAAGT GTCGGAGACT CAGAATCAGA
    GAAGCCTGGT TGCTGGGAAC AGCTCAAGGG GTCACCATGC TCTTCATAGT CACGGCCCTT
    CTCTGCTGTG GACTGTGCAA TGGGGTATTG ATAGAAGAGA CTGAAATAGT CATGCCAACC
    CCTAAGCCTG AGCTGTGGGC AGAGACCAAC TTTCCTCTGG CCCCGTGGAA GAACTTAACC
    CTCTGGTGCA GAAGCCCTTC TGGCTCAACT AAGGAGTTTG TGTTGCTGAA GGATGGGACC
    GGGTGGATCG CCACTCGCCC GGCCTCAGAG CAGGTCCGGG CTGCCTTCCC CCTTGGCGCC
    CTGACCCAGA GCCACACCGG GAGCTACCAC TGCCATTCAT GGGAGGAGAT GGCTGTATCG
    GAGCCCAGTG AGGCACTTGA GCTGGTGGGG ACAGACATCC TCCCCAAACC TGTCATTTCT
    GCTTCCCCCA CAATCCGGGG CCAGGAACTA CAACTCCGGT GCAAAGGATG GCTGGCAGGC
    ATGGGGTTTG CTCTGTATAA GGAGGGAGAG CAAGAACCTG TCCAGCAACT TGGTGCTGTT
    GGAAGAGAAG CCTTCTTTAC AATCCAGAGA ATGGAGGATA AAGACGAAGG CAATTACAGC
    TGCCGCACTC ACACTGAAAA ACGCCCCTTC AAGTGGTCTG AGCCCAGTGA GCCGCTGGAG
    CTTGTCATAA AAGAAATGTA CCCTAAGCCC TTCTTCAAGA CATGGGCCAG CCCTGTGGTC
    ACCCCTGGTG CCCGAGTGAC TTTCAATTGC TCCACCCCCC ACCAGCATAT GAGCTTTATT
    CTTTACAAAG ATGGAAGTGA AATAGCATCC AGTGACAGGT CCTGGGCAAG TCCGGGGGCC
    AGTGCAGCTC ACTTTCTAAT CATTTCGGTG GGCATTGGTG ATGGAGGGAA TTACAGCTGC
    CGATATTATG ACTTTTCTAT CTGGTCTGAG CCCAGCGACC CTGTGGAGCT CGTGGTGACA
    GAATTCTACC CCAAACCCAC TCTCCTGGCA CAGCCAGGTC CTGTGGTGTT TCCTGGGAAG
    AGTGTGATCC TGCGCTGCCA AGGGACTTTC CAGGGCATGA GGTTCGCCCT CTTGCAGGAG
    GGAGCCCATG TTCCCTTACA GTTTCGGAGT GTCTCAGGGA ACTCAGCTGA CTTCCTTCTC
    CACACTGTTG GAGCAGAGGA CTCTGGGAAC TATAGCTGTA TCTACTATGA GACAACCATG
    TCAAACAGGG GGTCATATCT CAGTATGCCC CTTATGATCT GGGTGACTGA CACATTCCCT
    AAGCCATGGT TGTTTGCTGA GCCCAGTTCT GTGGTTCCCA TGGGGCAGAA TGTTACTCTC
    TGGTGCCGAG GGCCGGTCCA TGGAGTAGGA TACATTCTGC ACAAAGAAGG AGAAGCCACT
    TCAATGCAGC TCTGGGGATC CACCAGTAAT GACGGGGCAT TCCCCATCAC CAATATATCT
    GGTACTAGCA TGGGGCGTTA CAGCTGCTGC TACCACCCTG ACTGGACCAG TTCTATCAAG
    ATACAACCTA GCAACACCCT GGAACTCCTA GTCACAGGCT TACTCCCCAA ACCCAGCCTA
    TTAGCCCAGC CTGGTCCCAT GGTGGCCCCT GGCGAAAATA TGACTCTTCA GTGTCAAGGG
    GAACTGCCAG ACTCAACATT TGTCCTGTTG AAGGAGGGGG CTCAGGAGCC TTTAGAGCAA
    CAGAGGCCAA GTGGGTACAG GGCTGACTTC TGGATGCCAG CAGTGAGAGG TGAAGACTCT
    GGGATCTATA GCTGTGTTTA TTATTTGGAC TCTACTCCCT TTGCAGCTTC AAATCACAGT
    GACTCCCTGG AGATCTGGGT GACTGATAAG CCCCCTAAAC CCTCTCTGTC AGCCTGGCCC
    AGCACCATGT TCAAGTTAGG GAAGGACATC ACCCTTCAGT GCCGAGGACC CCTGCCAGGT
    GTTGAATTTG TCCTAGAACA TGATGGAGAA GAAGCACCTC AGCAGTTTTC AGAGGATGGA
    GACTTTGTCA TCAACAACGT AGAAGGAAAA GGCATTGGAA ACTACAGCTG CAGCTACCGC
    CTCCAGGCCT ACCCTGATAT CTGGTCAGAG CCTAGTGATC CCCTGGAGCT GGTGGGGGCA
    GCAGGGCCTG TTGCTCAGGA GTGCACTGTA GGGAACATTG TCCGAAGTAG CCTAATCGTG
    GTGGTTGTTG TAGCCTTGGG GGTAGTGCTA GCCATAGAGT GGAAGAAGTG GCCTCGACTG
    CGAACCAGAG GCTCAGAGAC AGACGGAAGA GACCAGACCA TTGCCCTTGA AGAGTGTAAC
    CAAGAAGGAG AACCAGGCAC CCCTGCCAAT TCTCCTTCAT CAACCTCTCA GAGAATCTCT
    GTGGAACTGC CCGTTCCAAT ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58380
    Clone ID Related Accession (Same CDS sequence) XM_011531333.1 , XM_011531333.2
    Accession Version XM_011531333.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGC TTCAAATCAC AGTGACTCCC TGGAGATCTG GGTGACTGAT
    AAGCCCCCTA AACCCTCTCT GTCAGCCTGG CCCAGCACCA TGTTCAAGTT AGGGAAGGAC
    ATCACCCTTC AGTGCCGAGG ACCCCTGCCA GGTGTTGAAT TTGTCCTAGA ACATGATGGA
    GAAGAAGCAC CTCAGCAGTT TTCAGAGGAT GGAGACTTTG TCATCAACAA CGTAGAAGGA
    AAAGGCATTG GAAACTACAG CTGCAGCTAC CGCCTCCAGG CCTACCCTGA TATCTGGTCA
    GAGCCTAGTG ATCCCCTGGA GCTGGTGGGG GCAGCAGGGC CTGTTGCTCA GGAGTGCACT
    GTAGGGAACA TTGTCCGAAG TAGCCTAATC GTGGTGGTTG TTGTAGCCTT GGGGGTAGTG
    CTAGCCATAG AGTGGAAGAA GTGGCCTCGA CTGCGAACCA GAGGCTCAGA GACAGACGGA
    AGAGACCAGA CCATTGCCCT TGAAGAGTGT AACCAAGAAG GAGAACCAGG CACCCCTGCC
    AATTCTCCTT CATCAACCTC TCAGAGAATC TCTGTGGAAC TGCCCGTTCC AATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529635.1
    CDS284..4060
    Translation

    Target ORF information:

    RefSeq Version XM_011531333.1
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531333.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGC TTCAAATCAC AGTGACTCCC TGGAGATCTG GGTGACTGAT
    AAGCCCCCTA AACCCTCTCT GTCAGCCTGG CCCAGCACCA TGTTCAAGTT AGGGAAGGAC
    ATCACCCTTC AGTGCCGAGG ACCCCTGCCA GGTGTTGAAT TTGTCCTAGA ACATGATGGA
    GAAGAAGCAC CTCAGCAGTT TTCAGAGGAT GGAGACTTTG TCATCAACAA CGTAGAAGGA
    AAAGGCATTG GAAACTACAG CTGCAGCTAC CGCCTCCAGG CCTACCCTGA TATCTGGTCA
    GAGCCTAGTG ATCCCCTGGA GCTGGTGGGG GCAGCAGGGC CTGTTGCTCA GGAGTGCACT
    GTAGGGAACA TTGTCCGAAG TAGCCTAATC GTGGTGGTTG TTGTAGCCTT GGGGGTAGTG
    CTAGCCATAG AGTGGAAGAA GTGGCCTCGA CTGCGAACCA GAGGCTCAGA GACAGACGGA
    AGAGACCAGA CCATTGCCCT TGAAGAGTGT AACCAAGAAG GAGAACCAGG CACCCCTGCC
    AATTCTCCTT CATCAACCTC TCAGAGAATC TCTGTGGAAC TGCCCGTTCC AATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58380
    Clone ID Related Accession (Same CDS sequence) XM_011531333.1 , XM_011531333.2
    Accession Version XM_011531333.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3777bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011531333.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGC TTCAAATCAC AGTGACTCCC TGGAGATCTG GGTGACTGAT
    AAGCCCCCTA AACCCTCTCT GTCAGCCTGG CCCAGCACCA TGTTCAAGTT AGGGAAGGAC
    ATCACCCTTC AGTGCCGAGG ACCCCTGCCA GGTGTTGAAT TTGTCCTAGA ACATGATGGA
    GAAGAAGCAC CTCAGCAGTT TTCAGAGGAT GGAGACTTTG TCATCAACAA CGTAGAAGGA
    AAAGGCATTG GAAACTACAG CTGCAGCTAC CGCCTCCAGG CCTACCCTGA TATCTGGTCA
    GAGCCTAGTG ATCCCCTGGA GCTGGTGGGG GCAGCAGGGC CTGTTGCTCA GGAGTGCACT
    GTAGGGAACA TTGTCCGAAG TAGCCTAATC GTGGTGGTTG TTGTAGCCTT GGGGGTAGTG
    CTAGCCATAG AGTGGAAGAA GTGGCCTCGA CTGCGAACCA GAGGCTCAGA GACAGACGGA
    AGAGACCAGA CCATTGCCCT TGAAGAGTGT AACCAAGAAG GAGAACCAGG CACCCCTGCC
    AATTCTCCTT CATCAACCTC TCAGAGAATC TCTGTGGAAC TGCCCGTTCC AATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529635.1
    CDS158..3934
    Translation

    Target ORF information:

    RefSeq Version XM_011531333.2
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531333.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGC TTCAAATCAC AGTGACTCCC TGGAGATCTG GGTGACTGAT
    AAGCCCCCTA AACCCTCTCT GTCAGCCTGG CCCAGCACCA TGTTCAAGTT AGGGAAGGAC
    ATCACCCTTC AGTGCCGAGG ACCCCTGCCA GGTGTTGAAT TTGTCCTAGA ACATGATGGA
    GAAGAAGCAC CTCAGCAGTT TTCAGAGGAT GGAGACTTTG TCATCAACAA CGTAGAAGGA
    AAAGGCATTG GAAACTACAG CTGCAGCTAC CGCCTCCAGG CCTACCCTGA TATCTGGTCA
    GAGCCTAGTG ATCCCCTGGA GCTGGTGGGG GCAGCAGGGC CTGTTGCTCA GGAGTGCACT
    GTAGGGAACA TTGTCCGAAG TAGCCTAATC GTGGTGGTTG TTGTAGCCTT GGGGGTAGTG
    CTAGCCATAG AGTGGAAGAA GTGGCCTCGA CTGCGAACCA GAGGCTCAGA GACAGACGGA
    AGAGACCAGA CCATTGCCCT TGAAGAGTGT AACCAAGAAG GAGAACCAGG CACCCCTGCC
    AATTCTCCTT CATCAACCTC TCAGAGAATC TCTGTGGAAC TGCCCGTTCC AATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58381
    Clone ID Related Accession (Same CDS sequence) XM_011531334.1 , XM_011531334.2
    Accession Version XM_011531334.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GATAAGCCCC CTAAACCCTC TCTGTCAGCC
    TGGCCCAGCA CCATGTTCAA GTTAGGGAAG GACATCACCC TTCAGTGCCG AGGACCCCTG
    CCAGGTGTTG AATTTGTCCT AGAACATGAT GGAGAAGAAG CACCTCAGCA GTTTTCAGAG
    GATGGAGACT TTGTCATCAA CAACGTAGAA GGAAAAGGCA TTGGAAACTA CAGCTGCAGC
    TACCGCCTCC AGGCCTACCC TGATATCTGG TCAGAGCCTA GTGATCCCCT GGAGCTGGTG
    GGGGCAGCAG GGCCTGTTGC TCAGGAGTGC ACTGTAGGGA ACATTGTCCG AAGTAGCCTA
    ATCGTGGTGG TTGTTGTAGC CTTGGGGGTA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT
    CGACTGCGAA CCAGAGGCTC AGAGACAGAC GGAAGAGACC AGACCATTGC CCTTGAAGAG
    TGTAACCAAG AAGGAGAACC AGGCACCCCT GCCAATTCTC CTTCATCAAC CTCTCAGAGA
    ATCTCTGTGG AACTGCCCGT TCCAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529636.1
    CDS284..3733
    Translation

    Target ORF information:

    RefSeq Version XM_011531334.1
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531334.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GATAAGCCCC CTAAACCCTC TCTGTCAGCC
    TGGCCCAGCA CCATGTTCAA GTTAGGGAAG GACATCACCC TTCAGTGCCG AGGACCCCTG
    CCAGGTGTTG AATTTGTCCT AGAACATGAT GGAGAAGAAG CACCTCAGCA GTTTTCAGAG
    GATGGAGACT TTGTCATCAA CAACGTAGAA GGAAAAGGCA TTGGAAACTA CAGCTGCAGC
    TACCGCCTCC AGGCCTACCC TGATATCTGG TCAGAGCCTA GTGATCCCCT GGAGCTGGTG
    GGGGCAGCAG GGCCTGTTGC TCAGGAGTGC ACTGTAGGGA ACATTGTCCG AAGTAGCCTA
    ATCGTGGTGG TTGTTGTAGC CTTGGGGGTA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT
    CGACTGCGAA CCAGAGGCTC AGAGACAGAC GGAAGAGACC AGACCATTGC CCTTGAAGAG
    TGTAACCAAG AAGGAGAACC AGGCACCCCT GCCAATTCTC CTTCATCAAC CTCTCAGAGA
    ATCTCTGTGG AACTGCCCGT TCCAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58381
    Clone ID Related Accession (Same CDS sequence) XM_011531334.1 , XM_011531334.2
    Accession Version XM_011531334.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3450bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011531334.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GATAAGCCCC CTAAACCCTC TCTGTCAGCC
    TGGCCCAGCA CCATGTTCAA GTTAGGGAAG GACATCACCC TTCAGTGCCG AGGACCCCTG
    CCAGGTGTTG AATTTGTCCT AGAACATGAT GGAGAAGAAG CACCTCAGCA GTTTTCAGAG
    GATGGAGACT TTGTCATCAA CAACGTAGAA GGAAAAGGCA TTGGAAACTA CAGCTGCAGC
    TACCGCCTCC AGGCCTACCC TGATATCTGG TCAGAGCCTA GTGATCCCCT GGAGCTGGTG
    GGGGCAGCAG GGCCTGTTGC TCAGGAGTGC ACTGTAGGGA ACATTGTCCG AAGTAGCCTA
    ATCGTGGTGG TTGTTGTAGC CTTGGGGGTA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT
    CGACTGCGAA CCAGAGGCTC AGAGACAGAC GGAAGAGACC AGACCATTGC CCTTGAAGAG
    TGTAACCAAG AAGGAGAACC AGGCACCCCT GCCAATTCTC CTTCATCAAC CTCTCAGAGA
    ATCTCTGTGG AACTGCCCGT TCCAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529636.1
    CDS158..3607
    Translation

    Target ORF information:

    RefSeq Version XM_011531334.2
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531334.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GATAAGCCCC CTAAACCCTC TCTGTCAGCC
    TGGCCCAGCA CCATGTTCAA GTTAGGGAAG GACATCACCC TTCAGTGCCG AGGACCCCTG
    CCAGGTGTTG AATTTGTCCT AGAACATGAT GGAGAAGAAG CACCTCAGCA GTTTTCAGAG
    GATGGAGACT TTGTCATCAA CAACGTAGAA GGAAAAGGCA TTGGAAACTA CAGCTGCAGC
    TACCGCCTCC AGGCCTACCC TGATATCTGG TCAGAGCCTA GTGATCCCCT GGAGCTGGTG
    GGGGCAGCAG GGCCTGTTGC TCAGGAGTGC ACTGTAGGGA ACATTGTCCG AAGTAGCCTA
    ATCGTGGTGG TTGTTGTAGC CTTGGGGGTA GTGCTAGCCA TAGAGTGGAA GAAGTGGCCT
    CGACTGCGAA CCAGAGGCTC AGAGACAGAC GGAAGAGACC AGACCATTGC CCTTGAAGAG
    TGTAACCAAG AAGGAGAACC AGGCACCCCT GCCAATTCTC CTTCATCAAC CTCTCAGAGA
    ATCTCTGTGG AACTGCCCGT TCCAATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22975
    Clone ID Related Accession (Same CDS sequence) NM_001170961.1
    Accession Version NM_001170961.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4026bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform 3 precursor
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL590806.6 and AK226008.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (3) represents the longest transcript and encodes the longest protein (isoform 3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK226008.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGG CTTACTCCCC AAACCCAGCC TATTAGCCCA GCCTGGTCCC
    ATGGTGGCCC CTGGCGAAAA TATGACTCTT CAGTGTCAAG GGGAACTGCC AGACTCAACA
    TTTGTCCTGT TGAAGGAGGG GGCTCAGGAG CCTTTAGAGC AACAGAGGCC AAGTGGGTAC
    AGGGCTGACT TCTGGATGCC AGCAGTGAGA GGTGAAGACT CTGGGATCTA TAGCTGTGTT
    TATTATTTGG ACTCTACTCC CTTTGCAGCT TCAAATCACA GTGACTCCCT GGAGATCTGG
    GTGACTGATA AGCCCCCTAA ACCCTCTCTG TCAGCCTGGC CCAGCACCAT GTTCAAGTTA
    GGGAAGGACA TCACCCTTCA GTGCCGAGGA CCCCTGCCAG GTGTTGAATT TGTCCTAGAA
    CATGATGGAG AAGAAGCACC TCAGCAGTTT TCAGAGGATG GAGACTTTGT CATCAACAAC
    GTAGAAGGAA AAGGCATTGG AAACTACAGC TGCAGCTACC GCCTCCAGGC CTACCCTGAT
    ATCTGGTCAG AGCCTAGTGA TCCCCTGGAG CTGGTGGGGG CAGCAGGGCC TGTTGCTCAG
    GAGTGCACTG TAGGGAACAT TGTCCGAAGT AGCCTAATCG TGGTGGTTGT TGTAGCCTTG
    GGGGTAGTGC TAGCCATAGA GTGGAAGAAG TGGCCTCGAC TGCGAACCAG AGGCTCAGAG
    ACAGACGGAA GAGACCAGAC CATTGCCCTT GAAGAGTGTA ACCAAGAAGG AGAACCAGGC
    ACCCCTGCCA ATTCTCCTTC ATCAACCTCT CAGAGAATCT CTGTGGAACT GCCCGTTCCA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001164432.1
    CDS284..4309
    Translation

    Target ORF information:

    RefSeq Version NM_001170961.1
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001170961.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCGATAGTGA TGGACCCTCA ACCGGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGG CTTACTCCCC AAACCCAGCC TATTAGCCCA GCCTGGTCCC
    ATGGTGGCCC CTGGCGAAAA TATGACTCTT CAGTGTCAAG GGGAACTGCC AGACTCAACA
    TTTGTCCTGT TGAAGGAGGG GGCTCAGGAG CCTTTAGAGC AACAGAGGCC AAGTGGGTAC
    AGGGCTGACT TCTGGATGCC AGCAGTGAGA GGTGAAGACT CTGGGATCTA TAGCTGTGTT
    TATTATTTGG ACTCTACTCC CTTTGCAGCT TCAAATCACA GTGACTCCCT GGAGATCTGG
    GTGACTGATA AGCCCCCTAA ACCCTCTCTG TCAGCCTGGC CCAGCACCAT GTTCAAGTTA
    GGGAAGGACA TCACCCTTCA GTGCCGAGGA CCCCTGCCAG GTGTTGAATT TGTCCTAGAA
    CATGATGGAG AAGAAGCACC TCAGCAGTTT TCAGAGGATG GAGACTTTGT CATCAACAAC
    GTAGAAGGAA AAGGCATTGG AAACTACAGC TGCAGCTACC GCCTCCAGGC CTACCCTGAT
    ATCTGGTCAG AGCCTAGTGA TCCCCTGGAG CTGGTGGGGG CAGCAGGGCC TGTTGCTCAG
    GAGTGCACTG TAGGGAACAT TGTCCGAAGT AGCCTAATCG TGGTGGTTGT TGTAGCCTTG
    GGGGTAGTGC TAGCCATAGA GTGGAAGAAG TGGCCTCGAC TGCGAACCAG AGGCTCAGAG
    ACAGACGGAA GAGACCAGAC CATTGCCCTT GAAGAGTGTA ACCAAGAAGG AGAACCAGGC
    ACCCCTGCCA ATTCTCCTTC ATCAACCTCT CAGAGAATCT CTGTGGAACT GCCCGTTCCA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu58378
    Clone ID Related Accession (Same CDS sequence) XM_011531330.1
    Accession Version XM_011531330.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4026bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product immunoglobulin superfamily member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGG CTTACTCCCC AAACCCAGCC TATTAGCCCA GCCTGGTCCC
    ATGGTGGCCC CTGGCGAAAA TATGACTCTT CAGTGTCAAG GGGAACTGCC AGACTCAACA
    TTTGTCCTGT TGAAGGAGGG GGCTCAGGAG CCTTTAGAGC AACAGAGGCC AAGTGGGTAC
    AGGGCTGACT TCTGGATGCC AGCAGTGAGA GGTGAAGACT CTGGGATCTA TAGCTGTGTT
    TATTATTTGG ACTCTACTCC CTTTGCAGCT TCAAATCACA GTGACTCCCT GGAGATCTGG
    GTGACTGATA AGCCCCCTAA ACCCTCTCTG TCAGCCTGGC CCAGCACCAT GTTCAAGTTA
    GGGAAGGACA TCACCCTTCA GTGCCGAGGA CCCCTGCCAG GTGTTGAATT TGTCCTAGAA
    CATGATGGAG AAGAAGCACC TCAGCAGTTT TCAGAGGATG GAGACTTTGT CATCAACAAC
    GTAGAAGGAA AAGGCATTGG AAACTACAGC TGCAGCTACC GCCTCCAGGC CTACCCTGAT
    ATCTGGTCAG AGCCTAGTGA TCCCCTGGAG CTGGTGGGGG CAGCAGGGCC TGTTGCTCAG
    GAGTGCACTG TAGGGAACAT TGTCCGAAGT AGCCTAATCG TGGTGGTTGT TGTAGCCTTG
    GGGGTAGTGC TAGCCATAGA GTGGAAGAAG TGGCCTCGAC TGCGAACCAG AGGCTCAGAG
    ACAGACGGAA GAGACCAGAC CATTGCCCTT GAAGAGTGTA ACCAAGAAGG AGAACCAGGC
    ACCCCTGCCA ATTCTCCTTC ATCAACCTCT CAGAGAATCT CTGTGGAACT GCCCGTTCCA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011529632.1
    CDS182..4207
    Translation

    Target ORF information:

    RefSeq Version XM_011531330.1
    Organism Homo sapiens(human)
    Definition Homo sapiens immunoglobulin superfamily member 1 (IGSF1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011531330.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGACCCTGG ACAGACCAGG GGAGGGGGCC ACCATGCTGA AGACATTCAC TGTTTTGCTC 
    TTTTGCATTC GGATGAGTCT GGGTATGACA TCAATAGTGA TGGACCCTCA ACCAGAGTTG
    TGGATAGAGT CCAACTACCC CCAGGCCCCT TGGGAGAACA TCACGCTTTG GTGCCGAAGC
    CCCTCTCGGA TATCAAGCAA GTTCCTGCTG CTGAAGGATA AGACACAGAT GACCTGGATC
    CGCCCTTCCC ACAAGACCTT CCAAGTTTCA TTCCTTATAG GTGCCCTTAC TGAGTCCAAT
    GCAGGTCTTT ACCGGTGCTG CTACTGGAAG GAGACAGGCT GGTCAAAGCC CAGTAAAGTT
    CTAGAGTTGG AGGCACCAGG CCAACTGCCC AAGCCCATCT TCTGGATTCA GGCTGAGACC
    CCCGCTCTTC CTGGGTGTAA TGTTAACATC CTCTGCCATG GCTGGCTGCA GGATTTGGTA
    TTCATGCTGT TTAAAGAGGG ATATGCAGAG CCTGTGGATT ACCAAGTCCC AACTGGGACA
    ATGGCCATAT TCTCCATTGA CAACCTGACA CCTGAGGATG AAGGGGTTTA CATCTGCCGC
    ACTCATATCC AGATGCTCCC CACCCTGTGG TCAGAGCCCA GCAACCCCCT GAAGCTGGTT
    GTAGCAGGAC TCTACCCCAA ACCAACTTTG ACAGCCCATC CTGGGCCCAT CATGGCACCT
    GGAGAAAGCC TGAATCTCAG GTGCCAAGGG CCAATCTATG GAATGACCTT TGCTCTAATG
    AGGGTTGAAG ACTTGGAGAA GTCCTTTTAC CACAAGAAGA CAATAAAAAA TGAGGCAAAT
    TTCTTCTTCC AGTCTTTGAA GATCCAAGAT ACTGGACATT ACCTCTGTTT TTACTATGAC
    GCATCATATA GAGGTTCACT CCTTAGTGAT GTCCTGAAAA TCTGGGTGAC TGACACTTTC
    CCCAAGACCT GGCTACTTGC TCGGCCCAGT GCTGTGGTCC AAATGGGTCA GAATGTGAGC
    CTACGGTGTC GAGGACCAGT GGATGGAGTG GGTCTTGCAC TCTATAAGAA AGGAGAAGAC
    AAACCACTTC AATTTTTGGA TGCCACCAGC ATCGATGACA ACACATCATT CTTCCTCAAC
    AATGTAACCT ACAGTGATAC TGGCATCTAT AGCTGCCACT ATCTTCTCAC CTGGAAGACC
    TCCATTAGGA TGCCATCACA CAACACTGTG GAGCTTATGG TTGTAGATAA GCCCCCCAAA
    CCCTCCCTGT CAGCTTGGCC AAGCACTGTG TTCAAGCTAG GAAAGGCCAT CACCCTTCAG
    TGCCGAGTAT CTCATCCAGT ACTGGAATTT TCTCTGGAAT GGGAAGAAAG AGAAACATTC
    CAAAAATTCT CAGTAAACGG AGACTTCATC ATCAGTAATG TTGACGGGAA AGGCACAGGG
    ACCTACAGTT GCAGCTATCG CGTAGAGACA CATCCTAACA TCTGGTCACA TCGCAGTGAG
    CCCCTGAAGC TGATGGGGCC AGCAGGCTAT CTCACCTGGA ATTACGTTCT GAATGAAGCT
    ATCAGGTTGT CTCTAATCAT GCAGCTTGTT GCCTTGCTGT TGGTAGTGCT GTGGATAAGG
    TGGAAGTGTC GGAGACTCAG AATCAGAGAA GCCTGGTTGC TGGGAACAGC TCAAGGGGTC
    ACCATGCTCT TCATAGTCAC GGCCCTTCTC TGCTGTGCAA TTTCTTTTGC AGGACTGTGC
    AATGGGGTAT TGATAGAAGA GACTGAAATA GTCATGCCAA CCCCTAAGCC TGAGCTGTGG
    GCAGAGACCA ACTTTCCTCT GGCCCCGTGG AAGAACTTAA CCCTCTGGTG CAGAAGCCCT
    TCTGGCTCAA CTAAGGAGTT TGTGTTGCTG AAGGATGGGA CCGGGTGGAT CGCCACTCGC
    CCGGCCTCAG AGCAGGTCCG GGCTGCCTTC CCCCTTGGCG CCCTGACCCA GAGCCACACC
    GGGAGCTACC ACTGCCATTC ATGGGAGGAG ATGGCTGTAT CGGAGCCCAG TGAGGCACTT
    GAGCTGGTGG GGACAGACAT CCTCCCCAAA CCTGTCATTT CTGCTTCCCC CACAATCCGG
    GGCCAGGAAC TACAACTCCG GTGCAAAGGA TGGCTGGCAG GCATGGGGTT TGCTCTGTAT
    AAGGAGGGAG AGCAAGAACC TGTCCAGCAA CTTGGTGCTG TTGGAAGAGA AGCCTTCTTT
    ACAATCCAGA GAATGGAGGA TAAAGACGAA GGCAATTACA GCTGCCGCAC TCACACTGAA
    AAACGCCCCT TCAAGTGGTC TGAGCCCAGT GAGCCGCTGG AGCTTGTCAT AAAAGAAATG
    TACCCTAAGC CCTTCTTCAA GACATGGGCC AGCCCTGTGG TCACCCCTGG TGCCCGAGTG
    ACTTTCAATT GCTCCACCCC CCACCAGCAT ATGAGCTTTA TTCTTTACAA AGATGGAAGT
    GAAATAGCAT CCAGTGACAG GTCCTGGGCA AGTCCGGGGG CCAGTGCAGC TCACTTTCTA
    ATCATTTCGG TGGGCATTGG TGATGGAGGG AATTACAGCT GCCGATATTA TGACTTTTCT
    ATCTGGTCTG AGCCCAGCGA CCCTGTGGAG CTCGTGGTGA CAGAATTCTA CCCCAAACCC
    ACTCTCCTGG CACAGCCAGG TCCTGTGGTG TTTCCTGGGA AGAGTGTGAT CCTGCGCTGC
    CAAGGGACTT TCCAGGGCAT GAGGTTCGCC CTCTTGCAGG AGGGAGCCCA TGTTCCCTTA
    CAGTTTCGGA GTGTCTCAGG GAACTCAGCT GACTTCCTTC TCCACACTGT TGGAGCAGAG
    GACTCTGGGA ACTATAGCTG TATCTACTAT GAGACAACCA TGTCAAACAG GGGGTCATAT
    CTCAGTATGC CCCTTATGAT CTGGGTGACT GACACATTCC CTAAGCCATG GTTGTTTGCT
    GAGCCCAGTT CTGTGGTTCC CATGGGGCAG AATGTTACTC TCTGGTGCCG AGGGCCGGTC
    CATGGAGTAG GATACATTCT GCACAAAGAA GGAGAAGCCA CTTCAATGCA GCTCTGGGGA
    TCCACCAGTA ATGACGGGGC ATTCCCCATC ACCAATATAT CTGGTACTAG CATGGGGCGT
    TACAGCTGCT GCTACCACCC TGACTGGACC AGTTCTATCA AGATACAACC TAGCAACACC
    CTGGAACTCC TAGTCACAGG CTTACTCCCC AAACCCAGCC TATTAGCCCA GCCTGGTCCC
    ATGGTGGCCC CTGGCGAAAA TATGACTCTT CAGTGTCAAG GGGAACTGCC AGACTCAACA
    TTTGTCCTGT TGAAGGAGGG GGCTCAGGAG CCTTTAGAGC AACAGAGGCC AAGTGGGTAC
    AGGGCTGACT TCTGGATGCC AGCAGTGAGA GGTGAAGACT CTGGGATCTA TAGCTGTGTT
    TATTATTTGG ACTCTACTCC CTTTGCAGCT TCAAATCACA GTGACTCCCT GGAGATCTGG
    GTGACTGATA AGCCCCCTAA ACCCTCTCTG TCAGCCTGGC CCAGCACCAT GTTCAAGTTA
    GGGAAGGACA TCACCCTTCA GTGCCGAGGA CCCCTGCCAG GTGTTGAATT TGTCCTAGAA
    CATGATGGAG AAGAAGCACC TCAGCAGTTT TCAGAGGATG GAGACTTTGT CATCAACAAC
    GTAGAAGGAA AAGGCATTGG AAACTACAGC TGCAGCTACC GCCTCCAGGC CTACCCTGAT
    ATCTGGTCAG AGCCTAGTGA TCCCCTGGAG CTGGTGGGGG CAGCAGGGCC TGTTGCTCAG
    GAGTGCACTG TAGGGAACAT TGTCCGAAGT AGCCTAATCG TGGTGGTTGT TGTAGCCTTG
    GGGGTAGTGC TAGCCATAGA GTGGAAGAAG TGGCCTCGAC TGCGAACCAG AGGCTCAGAG
    ACAGACGGAA GAGACCAGAC CATTGCCCTT GAAGAGTGTA ACCAAGAAGG AGAACCAGGC
    ACCCCTGCCA ATTCTCCTTC ATCAACCTCT CAGAGAATCT CTGTGGAACT GCCCGTTCCA
    ATATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.