GJC3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GJC3
Entrez Gene ID	349149
Full Name	gap junction protein gamma 3
Synonyms	CX29,CX30.2,CX31.3,GJE1
General protein information	Preferred Names gap junction protein gamma 3 Names gap junction gamma-3 protein connexin 29 connexin-30.2 connexin-31.3 gap junction epsilon-1 protein gap junction protein, gamma 3, 30.2kDa
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	This gene encodes a gap junction protein. The encoded protein, also known as a connexin, plays a role in formation of gap junctions, which provide direct connections between neighboring cells. Mutations in this gene have been reported to be associated with nonsyndromic hearing loss.[provided by RefSeq, Feb 2010].
Disorder MIM:	611925

mRNA and Protein(s)

mRNA	Protein	Name
NM_181538.3	NP_853516.1	gap junction gamma-3 protein
NM_181538.2	NP_853516.1	gap junction gamma-3 protein

Pathways from BioSystems

Homologies

	LOC100424576	XP_002803266.1
Bos taurus (cattle)	GJC3	NP_001076962.1
Rattus norvegicus (Norway rat)	Gjc3	XP_006249079.1
Homo sapiens (human)	GJC3	NP_853516.1
Pan troglodytes (chimpanzee)	GJC3	XP_001143440.2
Canis lupus familiaris (dog)	GJC3	XP_005621129.1
Mus musculus (house mouse)	Gjc3	NP_536698.2

Gene Ontology
Bibliography

Related articles in PubMed

Functional analysis of a nonsyndromic hearing loss-associated mutation in the transmembrane II domain of the GJC3 gene.
Wong SH, Wang WH, Chen PH, Li SY, Yang JJ
International journal of medical sciences14(3)246-256(2017)

Mechanism of two novel human GJC3 missense mutations in causing non-syndromic hearing loss.
Su CC, Li SY, Yen YC, Nian JH, Liang WG, Yang JJ
Cell biochemistry and biophysics66(2)277-86(2013 Jun)

Human connexin30.2/31.3 (GJC3) does not form functional gap junction channels but causes enhanced ATP release in HeLa cells.
Liang WG, Su CC, Nian JH, Chiang AS, Li SY, Yang JJ
Cell biochemistry and biophysics61(1)189-97(2011 Sep)

The atrioventricular node: origin, development, and genetic program.
Bakker ML, Moorman AF, Christoffels VM
Trends in cardiovascular medicine20(5)164-71(2010 Jul)

A novel mutation in the connexin 29 gene may contribute to nonsyndromic hearing loss.
Hong HM, Yang JJ, Su CC, Chang JY, Li TC, Li SY
Human genetics127(2)191-9(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Based on these findings, we suggest that p.W77S mutant has a dominant negative effect on the formation and function of the gap junction. These results give a novel molecular elucidation for the mutation of GJC3 in the development of hearing loss.
Title: Functional analysis of a nonsyndromic hearing loss-associated mutation in the transmembrane II domain of the GJC3 gene.

Although p.R15G and p.L23H mutants do not decrease the trafficking of CX proteins, mutations in GJC3 genes result in a loss of hemichannel function of CX30.2/CX31.3 protein, possibly causing hearing loss.
Title: Mechanism of two novel human GJC3 missense mutations in causing non-syndromic hearing loss.

The connexin30.2/CX31.3 shares functional properties with pannexin (hemi) channels rather than gap junction channels of other Connexins.
Title: Human connexin30.2/31.3 (GJC3) does not form functional gap junction channels but causes enhanced ATP release in HeLa cells.

Observational study of gene-disease association. (HuGE Navigator)
Title: Prospective variants screening of connexin genes in children with hearing impairment: genotype/phenotype correlation.

Mutations in the Cx29 gene do not play a role in the causation of non syndromic hearing impairment in Indian population.
Title: Mutations in the connexin 29 gene are not a major cause of nonsyndromic hearing impairment in India.

The following GJC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GJC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17986	NM_181538.3 Latest version!	Homo sapiens gap junction protein gamma 3 (GJC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
View Old Accession Versions >>
OHu17986	NM_181538.2	Homo sapiens gap junction protein gamma 3 (GJC3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GJC3 ( NM_181538.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_853516.1 Homo sapiens gap junction protein gamma 3 (GJC3), mRNA.

Price & Availability↑

CloneID	OHu17986
Clone ID Related Accession (Same CDS sequence)	NM_181538.3 , NM_181538.2
Accession Version	NM_181538.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	gap junction gamma-3 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY297109.1, BC043381.1 and AC011904.3. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_181538.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY297109.1, AF503615.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312891.3/ ENSP00000325775.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGTGGCA GGTTCCTGCG GCGGCTGCTG GCGGAGGAGA GCCGGCGCTC CACCCCCGTG 
GGGCGCCTCT TGCTTCCCGT GCTCCTGGGA TTCCGCCTTG TGCTGCTGGC TGCCAGTGGG 
CCTGGAGTCT ATGGTGATGA GCAGAGTGAA TTCGTGTGTC ACACCCAGCA GCCGGGCTGC 
AAGGCTGCCT GCTTCGATGC CTTCCACCCC CTCTCCCCGC TGCGTTTCTG GGTCTTCCAG 
GTCATCTTGG TGGCTGTACC CAGCGCCCTC TATATGGGTT TCACTCTGTA TCACGTGATC 
TGGCACTGGG AATTATCAGG AAAGGGGAAG GAGGAGGAGA CCCTGATCCA GGGACGGGAG 
GGCAACACAG ATGTCCCAGG GGCTGGAAGC CTCAGGCTGC TCTGGGCTTA TGTGGCTCAG 
CTGGGGGCTC GGCTTGTCCT GGAGGGGGCA GCCCTGGGGT TGCAGTACCA CCTGTATGGG 
TTCCAGATGC CCAGCTCCTT TGCATGTCGC CGAGAACCTT GCCTTGGTAG TATAACCTGC 
AATCTGTCCC GCCCCTCTGA GAAGACCATT TTCCTAAAGA CCATGTTTGG AGTCAGCGGT 
TTCTGTCTCT TGTTTACTTT TTTGGAGCTT GTGCTTCTGG GTTTGGGGAG ATGGTGGAGG 
ACCTGGAAGC ACAAATCTTC CTCTTCTAAA TACTTCCTAA CTTCAGAGAG CACCAGAAGA 
CACAAGAAAG CAACCGATAG CCTCCCAGTG GTGGAAACCA AAGAGCAATT TCAAGAAGCA 
GTTCCAGGAA GAAGCTTAGC CCAGGAAAAA CAAAGACCAG TTGGACCCAG AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853516.1
CDS	1..840
Translation	MCGRFLRRLLAEESRRSTPVGRLLLPVLLGFRLVLLAASGPGVYGDEQSEFVCHTQQPGCKAACFDAFHPLSPLRFWVFQVILVAVPSALYMGFTLYHVIWHWELSGKGKEEETLIQGREGNTDVPGAGSLRLLWAYVAQLGARLVLEGAALGLQYHLYGFQMPSSFACRREPCLGSITCNLSRPSEKTIFLKTMFGVSGFCLLFTFLELVLLGLGRWWRTWKHKSSSSKYFLTSESTRRHKKATDSLPVVETKEQFQEAVPGRSLAQEKQRPVGPRDA

Target ORF information:

RefSeq Version	NM_181538.3
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein gamma 3 (GJC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181538.3

ORF Insert Sequence:

ATGTGTGGCA GGTTCCTGCG GCGGCTGCTG GCGGAGGAGA GCCGGCGCTC CACCCCCGTG 
GGGCGCCTCT TGCTTCCCGT GCTCCTGGGA TTCCGCCTTG TGCTGCTGGC TGCCAGTGGG 
CCTGGAGTCT ATGGTGATGA GCAGAGTGAA TTCGTGTGTC ACACCCAGCA GCCGGGCTGC 
AAGGCTGCCT GCTTCGATGC CTTCCACCCC CTCTCCCCGC TGCGTTTCTG GGTCTTCCAG 
GTCATCTTGG TGGCTGTACC CAGCGCCCTC TATATGGGTT TCACTCTGTA TCACGTGATC 
TGGCACTGGG AATTATCAGG AAAGGGGAAG GAGGAGGAGA CCCTGATCCA GGGACGGGAG 
GGCAACACAG ATGTCCCAGG GGCTGGAAGC CTCAGGCTGC TCTGGGCTTA TGTGGCTCAG 
CTGGGGGCTC GGCTTGTCCT GGAGGGGGCA GCCCTGGGGT TGCAGTACCA CCTGTATGGG 
TTCCAGATGC CCAGCTCCTT TGCATGTCGC CGAGAACCTT GCCTTGGTAG TATAACCTGC 
AATCTGTCCC GCCCCTCTGA GAAGACCATT TTCCTAAAGA CCATGTTTGG AGTCAGCGGT 
TTCTGTCTCT TGTTTACTTT TTTGGAGCTT GTGCTTCTGG GTTTGGGGAG ATGGTGGAGG 
ACCTGGAAGC ACAAATCTTC CTCTTCTAAA TACTTCCTAA CTTCAGAGAG CACCAGAAGA 
CACAAGAAAG CAACCGATAG CCTCCCAGTG GTGGAAACCA AAGAGCAATT TCAAGAAGCA 
GTTCCAGGAA GAAGCTTAGC CCAGGAAAAA CAAAGACCAG TTGGACCCAG AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GJC3 ( NM_181538.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_853516.1 Homo sapiens gap junction protein gamma 3 (GJC3), mRNA.

Price & Availability↑

CloneID	OHu17986
Clone ID Related Accession (Same CDS sequence)	NM_181538.3 , NM_181538.2
Accession Version	NM_181538.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 840bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	gap junction gamma-3 protein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY297109.1 and BC043381.1. This sequence is a reference standard in the RefSeqGene project. On Feb 19, 2010 this sequence version replaced NM_181538.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF503615.1, BX281800.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968832, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTGTGGCA GGTTCCTGCG GCGGCTGCTG GCGGAGGAGA GCCGGCGCTC CACCCCCGTG 
GGGCGCCTCT TGCTTCCCGT GCTCCTGGGA TTCCGCCTTG TGCTGCTGGC TGCCAGTGGG 
CCTGGAGTCT ATGGTGATGA GCAGAGTGAA TTCGTGTGTC ACACCCAGCA GCCGGGCTGC 
AAGGCTGCCT GCTTCGATGC CTTCCACCCC CTCTCCCCGC TGCGTTTCTG GGTCTTCCAG 
GTCATCTTGG TGGCTGTACC CAGCGCCCTC TATATGGGTT TCACTCTGTA TCACGTGATC 
TGGCACTGGG AATTATCAGG AAAGGGGAAG GAGGAGGAGA CCCTGATCCA GGGACGGGAG 
GGCAACACAG ATGTCCCAGG GGCTGGAAGC CTCAGGCTGC TCTGGGCTTA TGTGGCTCAG 
CTGGGGGCTC GGCTTGTCCT GGAGGGGGCA GCCCTGGGGT TGCAGTACCA CCTGTATGGG 
TTCCAGATGC CCAGCTCCTT TGCATGTCGC CGAGAACCTT GCCTTGGTAG TATAACCTGC 
AATCTGTCCC GCCCCTCTGA GAAGACCATT TTCCTAAAGA CCATGTTTGG AGTCAGCGGT 
TTCTGTCTCT TGTTTACTTT TTTGGAGCTT GTGCTTCTGG GTTTGGGGAG ATGGTGGAGG 
ACCTGGAAGC ACAAATCTTC CTCTTCTAAA TACTTCCTAA CTTCAGAGAG CACCAGAAGA 
CACAAGAAAG CAACCGATAG CCTCCCAGTG GTGGAAACCA AAGAGCAATT TCAAGAAGCA 
GTTCCAGGAA GAAGCTTAGC CCAGGAAAAA CAAAGACCAG TTGGACCCAG AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853516.1
CDS	1..840
Translation	MCGRFLRRLLAEESRRSTPVGRLLLPVLLGFRLVLLAASGPGVYGDEQSEFVCHTQQPGCKAACFDAFHPLSPLRFWVFQVILVAVPSALYMGFTLYHVIWHWELSGKGKEEETLIQGREGNTDVPGAGSLRLLWAYVAQLGARLVLEGAALGLQYHLYGFQMPSSFACRREPCLGSITCNLSRPSEKTIFLKTMFGVSGFCLLFTFLELVLLGLGRWWRTWKHKSSSSKYFLTSESTRRHKKATDSLPVVETKEQFQEAVPGRSLAQEKQRPVGPRDA

Target ORF information:

RefSeq Version	NM_181538.2
Organism	Homo sapiens(human)
Definition	Homo sapiens gap junction protein gamma 3 (GJC3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181538.2

ORF Insert Sequence:

ATGTGTGGCA GGTTCCTGCG GCGGCTGCTG GCGGAGGAGA GCCGGCGCTC CACCCCCGTG 
GGGCGCCTCT TGCTTCCCGT GCTCCTGGGA TTCCGCCTTG TGCTGCTGGC TGCCAGTGGG 
CCTGGAGTCT ATGGTGATGA GCAGAGTGAA TTCGTGTGTC ACACCCAGCA GCCGGGCTGC 
AAGGCTGCCT GCTTCGATGC CTTCCACCCC CTCTCCCCGC TGCGTTTCTG GGTCTTCCAG 
GTCATCTTGG TGGCTGTACC CAGCGCCCTC TATATGGGTT TCACTCTGTA TCACGTGATC 
TGGCACTGGG AATTATCAGG AAAGGGGAAG GAGGAGGAGA CCCTGATCCA GGGACGGGAG 
GGCAACACAG ATGTCCCAGG GGCTGGAAGC CTCAGGCTGC TCTGGGCTTA TGTGGCTCAG 
CTGGGGGCTC GGCTTGTCCT GGAGGGGGCA GCCCTGGGGT TGCAGTACCA CCTGTATGGG 
TTCCAGATGC CCAGCTCCTT TGCATGTCGC CGAGAACCTT GCCTTGGTAG TATAACCTGC 
AATCTGTCCC GCCCCTCTGA GAAGACCATT TTCCTAAAGA CCATGTTTGG AGTCAGCGGT 
TTCTGTCTCT TGTTTACTTT TTTGGAGCTT GTGCTTCTGG GTTTGGGGAG ATGGTGGAGG 
ACCTGGAAGC ACAAATCTTC CTCTTCTAAA TACTTCCTAA CTTCAGAGAG CACCAGAAGA 
CACAAGAAAG CAACCGATAG CCTCCCAGTG GTGGAAACCA AAGAGCAATT TCAAGAAGCA 
GTTCCAGGAA GAAGCTTAGC CCAGGAAAAA CAAAGACCAG TTGGACCCAG AGATGCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional analysis of a nonsyndromic hearing loss-associated mutation in the transmembrane II domain of the GJC3 gene.
International journal of medical sciences14(3)246-256(2017)
Wong SH,Wang WH,Chen PH,Li SY,Yang JJ

Mechanism of two novel human GJC3 missense mutations in causing non-syndromic hearing loss.
Cell biochemistry and biophysics66(2)277-86(2013 Jun)
Su CC,Li SY,Yen YC,Nian JH,Liang WG,Yang JJ

Human connexin30.2/31.3 (GJC3) does not form functional gap junction channels but causes enhanced ATP release in HeLa cells.
Cell biochemistry and biophysics61(1)189-97(2011 Sep)
Liang WG,Su CC,Nian JH,Chiang AS,Li SY,Yang JJ

The atrioventricular node: origin, development, and genetic program.
Trends in cardiovascular medicine20(5)164-71(2010 Jul)
Bakker ML,Moorman AF,Christoffels VM

A novel mutation in the connexin 29 gene may contribute to nonsyndromic hearing loss.
Human genetics127(2)191-9(2010 Feb)
Hong HM,Yang JJ,Su CC,Chang JY,Li TC,Li SY

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GJC3 cDNA ORF clone, Homo sapiens(human)

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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