QRFP cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	QRFP
Entrez Gene ID	347148
Full Name	pyroglutamylated RFamide peptide
Synonyms	26RFa,P518
General protein information	Preferred Names pyroglutamylated RFamide peptide Names orexigenic neuropeptide QRFP P518 precursor protein RF(Arg-Phe)amide family 26 amino acid peptide (P518) prepro-QRFP
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.12
Summary	This gene encodes a preproprotein that is proteolytically processed to generate multiple protein products. The encoded products are members of the RFamide family of neuropeptides, characterized by their common protein C-terminus consisting of an arginine (R) and an amidated phenylalanine (F). These products include the neuropeptides 26RFa and the N-terminally extended form, 43RFa. Both of these neuropeptides bind to the pyroglutamylated RFamide peptide receptor (QRFPR) and may regulate blood pressure, reproduction and food intake in rodents. [provided by RefSeq, Jul 2015].
Disorder MIM:	609795

mRNA and Protein(s)

mRNA	Protein	Name
NM_198180.3	NP_937823.1	orexigenic neuropeptide QRFP preproprotein
NM_198180.2	NP_937823.1	orexigenic neuropeptide QRFP preproprotein

Pathways from BioSystems

Homologies

	QRFP	XP_001106500.1
Canis lupus familiaris (dog)	QRFP	XP_850751.2
Bos taurus (cattle)	QRFP	NP_937865.1
Rattus norvegicus (Norway rat)	LOC100909783	XP_003749488.1
Pan troglodytes (chimpanzee)	QRFP	XP_001166241.3
Mus musculus (house mouse)	Qrfp	NP_906269.1
Homo sapiens (human)	QRFP	NP_937823.1
Rattus norvegicus (Norway rat)	Qrfp	NP_937843.1

Gene Ontology
Bibliography

Related articles in PubMed

Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.
Pr?vost G, Jeandel L, Arabo A, Co?ffier M, El Ouahli M, Picot M, Alexandre D, Gobet F, Leprince J, Berrahmoune H, D?chelotte P, Malagon M, Bonner C, Kerr-Conte J, Chigr F, Lefebvre H, Anouar Y, Chartrel N
Diabetes64(8)2805-16(2015 Aug)

Hypothalamic QRFP: regulation of food intake and fat selection.
Primeaux SD, Barnes MJ, Braymer HD
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(13)967-74(2013 Dec)

QRFP induces aldosterone production via PKC and T-type calcium channel-mediated pathways in human adrenocortical cells: evidence for a novel role of GPR103.
Ramanjaneya M, Karteris E, Chen J, Rucinski M, Ziolkowska A, Ahmed N, Kagerer S, J?hren O, Lehnert H, Malendowicz LK, Randeva HS
American journal of physiology. Endocrinology and metabolism305(9)E1049-58(2013 Nov)

The neuropeptide 26RFa is expressed in human prostate cancer and stimulates the neuroendocrine differentiation and the migration of androgeno-independent prostate cancer cells.
Alonzeau J, Alexandre D, Jeandel L, Courel M, Hautot C, El Yamani FZ, Gobet F, Leprince J, Magoul R, Amarti A, Pfister C, Yon L, Anouar Y, Chartrel N
European journal of cancer (Oxford, England : 1990)49(2)511-9(2013 Jan)

The RFamide neuropeptide 26RFa and its role in the control of neuroendocrine functions.
Chartrel N, Alonzeau J, Alexandre D, Jeandel L, Alvear-Perez R, Leprince J, Boutin J, Vaudry H, Anouar Y, Llorens-Cortes C
Frontiers in neuroendocrinology32(4)387-97(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show a moderate positive correlation between plasma 26RFa levels and insulin in patients with diabetes. Plasma 26RFa increases in response to glucose tolerance test. 26RFa and its receptor GPR103 are present in human pancreatic beta-cells and gut.
Title: Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.

Data indicate that 26RFa may participate to the development of prostate cancer at the androgen independent state by promoting the neuroendocrine differentiation and the migration of cancer cells via autocrine/paracrine mechanisms.
Title: The neuropeptide 26RFa is expressed in human prostate cancer and stimulates the neuroendocrine differentiation and the migration of androgeno-independent prostate cancer cells.

High levels of circulating 26RFa observed in anorexia nervosa patients might reflect an adaptive mechanism of the organism to promote energy intake and to increase fat stores in response to undernutrition.
Title: Orexigenic neuropeptide 26RFa: new evidence for an adaptive profile of appetite regulation in anorexia nervosa.

Results suggest that GPR103b and QRFP work in an autocrine/paracrine manner to regulate adipogenesis.
Title: GPR103b functions in the peripheral regulation of adipogenesis.

Characterization of 26RFa-related peptides showed that two distinct molecular forms of 26RFa are present in the human hypothalamus and spinal cord, i.e. 26RFa and an N-terminally elongated form of 43 amino acids designated 43RFa.
Title: Anatomical distribution and biochemical characterization of the novel RFamide peptide 26RFa in the human hypothalamus and spinal cord.

The following QRFP gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the QRFP cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25371	NM_198180.3 Latest version!	Homo sapiens pyroglutamylated RFamide peptide (QRFP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu25371	NM_198180.2	Homo sapiens pyroglutamylated RFamide peptide (QRFP), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

QRFP ( NM_198180.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_937823.1 Homo sapiens pyroglutamylated RFamide peptide (QRFP), mRNA.

Price & Availability↑

CloneID	OHu25371
Clone ID Related Accession (Same CDS sequence)	NM_198180.3 , NM_198180.2
Accession Version	NM_198180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	orexigenic neuropeptide QRFP preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CV575364.1, AL161733.20 and AB109625.1. On May 17, 2019 this sequence version replaced NM_198180.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CV575364.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2149178, SAMEA2153932 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal MANE Ensembl match :: ENST00000623824.2/ ENSP00000485512.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END##

ATGGTAAGGC CTTACCCCCT GATCTACTTC CTCTTCCTGC CGCTGGGCGC CTGCTTCCCT 
CTACTGGACA GAAGAGAGCC CACAGACGCC ATGGGTGGCC TCGGAGCTGG AGAACGCTGG 
GCCGACCTGG CCATGGGGCC CCGACCCCAC TCCGTGTGGG GTTCCTCTCG GTGGCTGAGA 
GCTTCACAGC CACAGGCCCT GCTTGTCATA GCCAGGGGGC TGCAGACATC GGGCAGAGAG 
CATGCTGGCT GCAGATTCCG CTTCGGGAGG CAGGACGAAG GCAGTGAGGC CACCGGCTTC 
CTCCCTGCTG CGGGGGAGAA GACCAGCGGC CCGTTAGGGA ACCTGGCTGA GGAGCTCAAT 
GGCTACAGCA GGAAGAAAGG CGGCTTCAGC TTCCGCTTCG GTCGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937823.1
CDS	522..932
Translation	MVRPYPLIYFLFLPLGACFPLLDRREPTDAMGGLGAGERWADLAMGPRPHSVWGSSRWLRASQPQALLVIARGLQTSGREHAGCRFRFGRQDEGSEATGFLPAAGEKTSGPLGNLAEELNGYSRKKGGFSFRFGRR

Target ORF information:

RefSeq Version	NM_198180.3
Organism	Homo sapiens(human)
Definition	Homo sapiens pyroglutamylated RFamide peptide (QRFP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198180.3

ORF Insert Sequence:

ATGGTAAGGC CTTACCCCCT GATCTACTTC CTCTTCCTGC CGCTGGGCGC CTGCTTCCCT 
CTACTGGACA GAAGAGAGCC CACAGACGCC ATGGGTGGCC TCGGAGCTGG AGAACGCTGG 
GCCGACCTGG CCATGGGGCC CCGACCCCAC TCCGTGTGGG GTTCCTCTCG GTGGCTGAGA 
GCTTCACAGC CACAGGCCCT GCTTGTCATA GCCAGGGGGC TGCAGACATC GGGCAGAGAG 
CATGCTGGCT GCAGATTCCG CTTCGGGAGG CAGGACGAAG GCAGTGAGGC CACCGGCTTC 
CTCCCTGCTG CGGGGGAGAA GACCAGCGGC CCGTTAGGGA ACCTGGCTGA GGAGCTCAAT 
GGCTACAGCA GGAAGAAAGG CGGCTTCAGC TTCCGCTTCG GTCGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

QRFP ( NM_198180.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_937823.1 Homo sapiens pyroglutamylated RFamide peptide (QRFP), mRNA.

Price & Availability↑

CloneID	OHu25371
Clone ID Related Accession (Same CDS sequence)	NM_198180.3 , NM_198180.2
Accession Version	NM_198180.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 411bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	orexigenic neuropeptide QRFP preproprotein
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CV575364.1, AL161733.20 and AB109625.1. On Aug 1, 2015 this sequence version replaced NM_198180.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CV575364.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2149178, SAMEA2153932 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: downstream AUG is associated with N-terminal localization signal ##RefSeq-Attributes-END##

ATGGTAAGGC CTTACCCCCT GATCTACTTC CTCTTCCTGC CGCTGGGCGC CTGCTTCCCT 
CTACTGGACA GAAGAGAGCC CACAGACGCC ATGGGTGGCC TCGGAGCTGG AGAACGCTGG 
GCCGACCTGG CCATGGGGCC CCGACCCCAC TCCGTGTGGG GTTCCTCTCG GTGGCTGAGA 
GCTTCACAGC CACAGGCCCT GCTTGTCATA GCCAGGGGGC TGCAGACATC GGGCAGAGAG 
CATGCTGGCT GCAGATTCCG CTTCGGGAGG CAGGACGAAG GCAGTGAGGC CACCGGCTTC 
CTCCCTGCTG CGGGGGAGAA GACCAGCGGC CCGTTAGGGA ACCTGGCTGA GGAGCTCAAT 
GGCTACAGCA GGAAGAAAGG CGGCTTCAGC TTCCGCTTCG GTCGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_937823.1
CDS	522..932
Translation	MVRPYPLIYFLFLPLGACFPLLDRREPTDAMGGLGAGERWADLAMGPRPHSVWGSSRWLRASQPQALLVIARGLQTSGREHAGCRFRFGRQDEGSEATGFLPAAGEKTSGPLGNLAEELNGYSRKKGGFSFRFGRR

Target ORF information:

RefSeq Version	NM_198180.2
Organism	Homo sapiens(human)
Definition	Homo sapiens pyroglutamylated RFamide peptide (QRFP), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198180.2

ORF Insert Sequence:

ATGGTAAGGC CTTACCCCCT GATCTACTTC CTCTTCCTGC CGCTGGGCGC CTGCTTCCCT 
CTACTGGACA GAAGAGAGCC CACAGACGCC ATGGGTGGCC TCGGAGCTGG AGAACGCTGG 
GCCGACCTGG CCATGGGGCC CCGACCCCAC TCCGTGTGGG GTTCCTCTCG GTGGCTGAGA 
GCTTCACAGC CACAGGCCCT GCTTGTCATA GCCAGGGGGC TGCAGACATC GGGCAGAGAG 
CATGCTGGCT GCAGATTCCG CTTCGGGAGG CAGGACGAAG GCAGTGAGGC CACCGGCTTC 
CTCCCTGCTG CGGGGGAGAA GACCAGCGGC CCGTTAGGGA ACCTGGCTGA GGAGCTCAAT 
GGCTACAGCA GGAAGAAAGG CGGCTTCAGC TTCCGCTTCG GTCGGCGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Hypothalamic Neuropeptide 26RFa Acts as an Incretin to Regulate Glucose Homeostasis.
Diabetes64(8)2805-16(2015 Aug)
Pr?vost G,Jeandel L,Arabo A,Co?ffier M,El Ouahli M,Picot M,Alexandre D,Gobet F,Leprince J,Berrahmoune H,D?chelotte P,Malagon M,Bonner C,Kerr-Conte J,Chigr F,Lefebvre H,Anouar Y,Chartrel N

Hypothalamic QRFP: regulation of food intake and fat selection.
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme45(13)967-74(2013 Dec)
Primeaux SD,Barnes MJ,Braymer HD

QRFP induces aldosterone production via PKC and T-type calcium channel-mediated pathways in human adrenocortical cells: evidence for a novel role of GPR103.
American journal of physiology. Endocrinology and metabolism305(9)E1049-58(2013 Nov)
Ramanjaneya M,Karteris E,Chen J,Rucinski M,Ziolkowska A,Ahmed N,Kagerer S,J?hren O,Lehnert H,Malendowicz LK,Randeva HS

The neuropeptide 26RFa is expressed in human prostate cancer and stimulates the neuroendocrine differentiation and the migration of androgeno-independent prostate cancer cells.
European journal of cancer (Oxford, England : 1990)49(2)511-9(2013 Jan)
Alonzeau J,Alexandre D,Jeandel L,Courel M,Hautot C,El Yamani FZ,Gobet F,Leprince J,Magoul R,Amarti A,Pfister C,Yon L,Anouar Y,Chartrel N

The RFamide neuropeptide 26RFa and its role in the control of neuroendocrine functions.
Frontiers in neuroendocrinology32(4)387-97(2011 Oct)
Chartrel N,Alonzeau J,Alexandre D,Jeandel L,Alvear-Perez R,Leprince J,Boutin J,Vaudry H,Anouar Y,Llorens-Cortes C

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QRFP cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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