MCIDAS cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MCIDAS
Entrez Gene ID	345643
Full Name	multiciliate differentiation and DNA synthesis associated cell cycle protein
Synonyms	IDAS,MCI,MCIN
General protein information	Preferred Names multiciliate differentiation and DNA synthesis associated cell cycle protein Names multicilin multiciliate cell differentiation 1 protein Idas
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q11.2
Summary	This gene encodes a member of the geminin family of proteins. The encoded nuclear protein is required for the generation of multiciliated cells in respiratory epithelium. Mutations in this gene cause a rare mucociliary clearance disorder associated with recurring respiratory infections in human patients, known as reduced generation of multiple motile cilia (RGMC). [provided by RefSeq, Sep 2016].
Disorder MIM:	614086

mRNA and Protein(s)

mRNA	Protein	Name
NM_001190787.2	NP_001177716.1	multicilin
XM_017009439.2	XP_016864928.1	multicilin isoform X1
NM_001190787.1	NP_001177716.1	multicilin
XM_017009439.1	XP_016864928.1	multicilin isoform X1
NM_001190787.3	NP_001177716.1	multicilin

Pathways from BioSystems

Homologies

Homo sapiens (human)	MCIDAS	NP_001177716.1
Pan troglodytes (chimpanzee)	MCIN	XP_001147303.2
Canis lupus familiaris (dog)	MCIDAS	XP_003433839.1
Xenopus tropicalis (tropical clawed frog)	mci	XP_002941156.1
	LOC706081	XP_001098119.2
Bos taurus (cattle)	MCIDAS	XP_002696376.1
Mus musculus (house mouse)	Mcidas	NP_001033003.1
Rattus norvegicus (Norway rat)	Mcidas	XP_001068366.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

MCIDAS mutations result in a mucociliary clearance disorder with reduced generation of multiple motile cilia.
Boon M, Wallmeier J, Ma L, Loges NT, Jaspers M, Olbrich H, Dougherty GW, Raidt J, Werner C, Amirav I, Hevroni A, Abitbul R, Avital A, Soferman R, Wessels M, O'Callaghan C, Chung EM, Rutman A, Hirst RA, Moya E, Mitchison HM, Van Daele S, De Boeck K, Jorissen M, Kintner C, Cuppens H, Omran H
Nature communications54418(2014 Jul)

The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing.
Caillat C, Pefani DE, Gillespie PJ, Taraviras S, Blow JJ, Lygerou Z, Perrakis A
The Journal of biological chemistry288(44)31624-34(2013 Nov)

Multicilin promotes centriole assembly and ciliogenesis during multiciliate cell differentiation.
Stubbs JL, Vladar EK, Axelrod JD, Kintner C
Nature cell biology14(2)140-7(2012 Jan)

Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression.
Pefani DE, Dimaki M, Spella M, Karantzelis N, Mitsiki E, Kyrousi C, Symeonidou IE, Perrakis A, Taraviras S, Lygerou Z
The Journal of biological chemistry286(26)23234-46(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

MCIDAS mutations result in a mucociliary clearance disorder with reduced generation of multiple motile cilia.MCIDAS regulates CCNO and FOXJ1 expression.
Title: MCIDAS mutations result in a mucociliary clearance disorder with reduced generation of multiple motile cilia.

The properties of the Idas-Geminin complex suggest it as the functional form of Idas and provide a possible mechanism to modulate Geminin activity
Title: The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing.

Idas as a novel Geminin binding partner, implicated in cell cycle progression, and a putative regulator of proliferation-differentiation decisions during development.
Title: Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression.

The following MCIDAS gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MCIDAS cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu00715	NM_001190787.3 Latest version!	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00
OHu95862	XM_017009439.2 Latest version!	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu00715	NM_001190787.1	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu95862	XM_017009439.1	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OHu00715	NM_001190787.2	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MCIDAS ( NM_001190787.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177716.1 Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.

Price & Availability↑

CloneID	OHu00715
Clone ID Related Accession (Same CDS sequence)	NM_001190787.2 , NM_001190787.1 , NM_001190787.3
Accession Version	NM_001190787.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-16
Organism	Homo sapiens(human)
Product	multicilin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091977.3. On May 17, 2019 this sequence version replaced NM_001190787.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BX383954.2, FR854393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGCAGGCGT GCGGGGGCGG CGCGGCCGGC CGTCGGGCCT TCGACAGCAT CTGCCCCAAC 
AGAATGCTGG CACTGCCGGG CCGGGCGCTG CTCTGCAAGC CGGGGAAGCC GGAGAGGAAG 
TTCGCTCCTC CGCGGAAGTT CTTCCCCGGA TGCACAGGCG GGAGCCCGGT GTCGGTGTAC 
GAGGATCCCC CGGACGCCGA GCCCACAGCG CTGCCAGCCC TCACCACCAT AGACCTGCAG 
GACCTCGCTG ACTGCTCTTC GCTACTCGGG TCCGACGCGC CGCCTGGTGG TGACCTGGCC 
GCCTCGCAGA ACCATTCCCA CCAAACGGAA GCAGACTTCA ATCTGCAAGA TTTCAGAGAC 
ACGGTGGATG ATCTCATTTC AGACTCATCC TCTATGATGT CGCCTACCCT GGCCAGCGGA 
GACTTCCCCT TCTCTCCTTG CGACATATCA CCATTCGGGC CCTGCCTCTC CCCGCCACTG 
GACCCACGGG CCCTGCAGTC ACCACCGCTG CGCCCTCCAG ACGTGCCCCC GCCTGAGCAA 
TACTGGAAGG AGGTGGCGGA CCAGAACCAG AGAGCGTTGG GAGACGCGCT TGTTGAGAAT 
AATCAACTGC ACGTGACATT GACCCAGAAA CAGGAGGAGA TCGCCTCGCT CAAGGAGCGG 
AACGTGCAGC TGAAGGAACT CGCCAGCCGA ACCCGGCACC TGGCCTCGGT GCTGGATAAG 
CTGATGATCA CACAGTCCCG GGATTGTGGG GCGGCGGCCG AGCCCTTCCT GCTCAAGGCG 
AAGGCCAAAA GGAGCCTGGA GGAGTTGGTC AGCGCTGCGG GGCAGGATTG CGCGGAAGTG 
GACGCCATCC TGAGGGAGAT TTCCGAGCGC TGCGATGAAG CCCTTCAGAG CCGCGATCCC 
AAGCGGCCCC GACTGCTGCC AGAGCCCGCG AATACTGACA CCAGGCCCGG GAACCTGCAT 
GGCGCCTTCC GGGGGCTGCG CACAGACTGC AGCCGGAGCG CGTTGAACCT GAGCCACAGT 
GAGCTGGAGG AGGGCGGCTC CTTCAGCACC CGCATCCGCA GCCACAGCAC CATCCGCACC 
CTCGCCTTCC CCCAGGGCAA TGCCTTCACC ATCAGAACAG CCAACGGGGG TTACAAGTTC 
CGCTGGGTCC CCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177716.1
CDS	178..1335
Translation	MQACGGGAAGRRAFDSICPNRMLALPGRALLCKPGKPERKFAPPRKFFPGCTGGSPVSVYEDPPDAEPTALPALTTIDLQDLADCSSLLGSDAPPGGDLAASQNHSHQTEADFNLQDFRDTVDDLISDSSSMMSPTLASGDFPFSPCDISPFGPCLSPPLDPRALQSPPLRPPDVPPPEQYWKEVADQNQRALGDALVENNQLHVTLTQKQEEIASLKERNVQLKELASRTRHLASVLDKLMITQSRDCGAAAEPFLLKAKAKRSLEELVSAAGQDCAEVDAILREISERCDEALQSRDPKRPRLLPEPANTDTRPGNLHGAFRGLRTDCSRSALNLSHSELEEGGSFSTRIRSHSTIRTLAFPQGNAFTIRTANGGYKFRWVPS

Target ORF information:

RefSeq Version	NM_001190787.2
Organism	Homo sapiens(human)
Definition	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190787.2

ORF Insert Sequence:

ATGCAGGCGT GCGGGGGCGG CGCGGCCGGC CGTCGGGCCT TCGACAGCAT CTGCCCCAAC 
AGAATGCTGG CACTGCCGGG CCGGGCGCTG CTCTGCAAGC CGGGGAAGCC GGAGAGGAAG 
TTCGCTCCTC CGCGGAAGTT CTTCCCCGGA TGCACAGGCG GGAGCCCGGT GTCGGTGTAC 
GAGGATCCCC CGGACGCCGA GCCCACAGCG CTGCCAGCCC TCACCACCAT AGACCTGCAG 
GACCTCGCTG ACTGCTCTTC GCTACTCGGG TCCGACGCGC CGCCTGGTGG TGACCTGGCC 
GCCTCGCAGA ACCATTCCCA CCAAACGGAA GCAGACTTCA ATCTGCAAGA TTTCAGAGAC 
ACGGTGGATG ATCTCATTTC AGACTCATCC TCTATGATGT CGCCTACCCT GGCCAGCGGA 
GACTTCCCCT TCTCTCCTTG CGACATATCA CCATTCGGGC CCTGCCTCTC CCCGCCACTG 
GACCCACGGG CCCTGCAGTC ACCACCGCTG CGCCCTCCAG ACGTGCCCCC GCCTGAGCAA 
TACTGGAAGG AGGTGGCGGA CCAGAACCAG AGAGCGTTGG GAGACGCGCT TGTTGAGAAT 
AATCAACTGC ACGTGACATT GACCCAGAAA CAGGAGGAGA TCGCCTCGCT CAAGGAGCGG 
AACGTGCAGC TGAAGGAACT CGCCAGCCGA ACCCGGCACC TGGCCTCGGT GCTGGATAAG 
CTGATGATCA CACAGTCCCG GGATTGTGGG GCGGCGGCCG AGCCCTTCCT GCTCAAGGCG 
AAGGCCAAAA GGAGCCTGGA GGAGTTGGTC AGCGCTGCGG GGCAGGATTG CGCGGAAGTG 
GACGCCATCC TGAGGGAGAT TTCCGAGCGC TGCGATGAAG CCCTTCAGAG CCGCGATCCC 
AAGCGGCCCC GACTGCTGCC AGAGCCCGCG AATACTGACA CCAGGCCCGG GAACCTGCAT 
GGCGCCTTCC GGGGGCTGCG CACAGACTGC AGCCGGAGCG CGTTGAACCT GAGCCACAGT 
GAGCTGGAGG AGGGCGGCTC CTTCAGCACC CGCATCCGCA GCCACAGCAC CATCCGCACC 
CTCGCCTTCC CCCAGGGCAA TGCCTTCACC ATCAGAACAG CCAACGGGGG TTACAAGTTC 
CGCTGGGTCC CCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCIDAS ( NM_001190787.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177716.1 Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.

Price & Availability↑

CloneID	OHu00715
Clone ID Related Accession (Same CDS sequence)	NM_001190787.2 , NM_001190787.1 , NM_001190787.3
Accession Version	NM_001190787.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Homo sapiens(human)
Product	multicilin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091977.3. This sequence is a reference standard in the RefSeqGene project. On or before Jun 30, 2010 this sequence version replaced XR_040411.2, XR_040412.2, XR_040410.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BX383954.2, FR854393.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144120 [ECO:0000348] ##Evidence-Data-END##

ATGCAGGCGT GCGGGGGCGG CGCGGCCGGC CGTCGGGCCT TCGACAGCAT CTGCCCCAAC 
AGAATGCTGG CACTGCCGGG CCGGGCGCTG CTCTGCAAGC CGGGGAAGCC GGAGAGGAAG 
TTCGCTCCTC CGCGGAAGTT CTTCCCCGGA TGCACAGGCG GGAGCCCGGT GTCGGTGTAC 
GAGGATCCCC CGGACGCCGA GCCCACAGCG CTGCCAGCCC TCACCACCAT AGACCTGCAG 
GACCTCGCTG ACTGCTCTTC GCTACTCGGG TCCGACGCGC CGCCTGGTGG TGACCTGGCC 
GCCTCGCAGA ACCATTCCCA CCAAACGGAA GCAGACTTCA ATCTGCAAGA TTTCAGAGAC 
ACGGTGGATG ATCTCATTTC AGACTCATCC TCTATGATGT CGCCTACCCT GGCCAGCGGA 
GACTTCCCCT TCTCTCCTTG CGACATATCA CCATTCGGGC CCTGCCTCTC CCCGCCACTG 
GACCCACGGG CCCTGCAGTC ACCACCGCTG CGCCCTCCAG ACGTGCCCCC GCCTGAGCAA 
TACTGGAAGG AGGTGGCGGA CCAGAACCAG AGAGCGTTGG GAGACGCGCT TGTTGAGAAT 
AATCAACTGC ACGTGACATT GACCCAGAAA CAGGAGGAGA TCGCCTCGCT CAAGGAGCGG 
AACGTGCAGC TGAAGGAACT CGCCAGCCGA ACCCGGCACC TGGCCTCGGT GCTGGATAAG 
CTGATGATCA CACAGTCCCG GGATTGTGGG GCGGCGGCCG AGCCCTTCCT GCTCAAGGCG 
AAGGCCAAAA GGAGCCTGGA GGAGTTGGTC AGCGCTGCGG GGCAGGATTG CGCGGAAGTG 
GACGCCATCC TGAGGGAGAT TTCCGAGCGC TGCGATGAAG CCCTTCAGAG CCGCGATCCC 
AAGCGGCCCC GACTGCTGCC AGAGCCCGCG AATACTGACA CCAGGCCCGG GAACCTGCAT 
GGCGCCTTCC GGGGGCTGCG CACAGACTGC AGCCGGAGCG CGTTGAACCT GAGCCACAGT 
GAGCTGGAGG AGGGCGGCTC CTTCAGCACC CGCATCCGCA GCCACAGCAC CATCCGCACC 
CTCGCCTTCC CCCAGGGCAA TGCCTTCACC ATCAGAACAG CCAACGGGGG TTACAAGTTC 
CGCTGGGTCC CCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177716.1
CDS	178..1335
Translation	MQACGGGAAGRRAFDSICPNRMLALPGRALLCKPGKPERKFAPPRKFFPGCTGGSPVSVYEDPPDAEPTALPALTTIDLQDLADCSSLLGSDAPPGGDLAASQNHSHQTEADFNLQDFRDTVDDLISDSSSMMSPTLASGDFPFSPCDISPFGPCLSPPLDPRALQSPPLRPPDVPPPEQYWKEVADQNQRALGDALVENNQLHVTLTQKQEEIASLKERNVQLKELASRTRHLASVLDKLMITQSRDCGAAAEPFLLKAKAKRSLEELVSAAGQDCAEVDAILREISERCDEALQSRDPKRPRLLPEPANTDTRPGNLHGAFRGLRTDCSRSALNLSHSELEEGGSFSTRIRSHSTIRTLAFPQGNAFTIRTANGGYKFRWVPS

Target ORF information:

RefSeq Version	NM_001190787.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190787.1

ORF Insert Sequence:

ATGCAGGCGT GCGGGGGCGG CGCGGCCGGC CGTCGGGCCT TCGACAGCAT CTGCCCCAAC 
AGAATGCTGG CACTGCCGGG CCGGGCGCTG CTCTGCAAGC CGGGGAAGCC GGAGAGGAAG 
TTCGCTCCTC CGCGGAAGTT CTTCCCCGGA TGCACAGGCG GGAGCCCGGT GTCGGTGTAC 
GAGGATCCCC CGGACGCCGA GCCCACAGCG CTGCCAGCCC TCACCACCAT AGACCTGCAG 
GACCTCGCTG ACTGCTCTTC GCTACTCGGG TCCGACGCGC CGCCTGGTGG TGACCTGGCC 
GCCTCGCAGA ACCATTCCCA CCAAACGGAA GCAGACTTCA ATCTGCAAGA TTTCAGAGAC 
ACGGTGGATG ATCTCATTTC AGACTCATCC TCTATGATGT CGCCTACCCT GGCCAGCGGA 
GACTTCCCCT TCTCTCCTTG CGACATATCA CCATTCGGGC CCTGCCTCTC CCCGCCACTG 
GACCCACGGG CCCTGCAGTC ACCACCGCTG CGCCCTCCAG ACGTGCCCCC GCCTGAGCAA 
TACTGGAAGG AGGTGGCGGA CCAGAACCAG AGAGCGTTGG GAGACGCGCT TGTTGAGAAT 
AATCAACTGC ACGTGACATT GACCCAGAAA CAGGAGGAGA TCGCCTCGCT CAAGGAGCGG 
AACGTGCAGC TGAAGGAACT CGCCAGCCGA ACCCGGCACC TGGCCTCGGT GCTGGATAAG 
CTGATGATCA CACAGTCCCG GGATTGTGGG GCGGCGGCCG AGCCCTTCCT GCTCAAGGCG 
AAGGCCAAAA GGAGCCTGGA GGAGTTGGTC AGCGCTGCGG GGCAGGATTG CGCGGAAGTG 
GACGCCATCC TGAGGGAGAT TTCCGAGCGC TGCGATGAAG CCCTTCAGAG CCGCGATCCC 
AAGCGGCCCC GACTGCTGCC AGAGCCCGCG AATACTGACA CCAGGCCCGG GAACCTGCAT 
GGCGCCTTCC GGGGGCTGCG CACAGACTGC AGCCGGAGCG CGTTGAACCT GAGCCACAGT 
GAGCTGGAGG AGGGCGGCTC CTTCAGCACC CGCATCCGCA GCCACAGCAC CATCCGCACC 
CTCGCCTTCC CCCAGGGCAA TGCCTTCACC ATCAGAACAG CCAACGGGGG TTACAAGTTC 
CGCTGGGTCC CCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCIDAS ( NM_001190787.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001177716.1 Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.

Price & Availability↑

CloneID	OHu00715
Clone ID Related Accession (Same CDS sequence)	NM_001190787.2 , NM_001190787.1 , NM_001190787.3
Accession Version	NM_001190787.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-19
Organism	Homo sapiens(human)
Product	multicilin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC091977.3. On Aug 31, 2019 this sequence version replaced NM_001190787.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BX383954.2, FR854393.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGCAGGCGT GCGGGGGCGG CGCGGCCGGC CGTCGGGCCT TCGACAGCAT CTGCCCCAAC 
AGAATGCTGG CACTGCCGGG CCGGGCGCTG CTCTGCAAGC CGGGGAAGCC GGAGAGGAAG 
TTCGCTCCTC CGCGGAAGTT CTTCCCCGGA TGCACAGGCG GGAGCCCGGT GTCGGTGTAC 
GAGGATCCCC CGGACGCCGA GCCCACAGCG CTGCCAGCCC TCACCACCAT AGACCTGCAG 
GACCTCGCTG ACTGCTCTTC GCTACTCGGG TCCGACGCGC CGCCTGGTGG TGACCTGGCC 
GCCTCGCAGA ACCATTCCCA CCAAACGGAA GCAGACTTCA ATCTGCAAGA TTTCAGAGAC 
ACGGTGGATG ATCTCATTTC AGACTCATCC TCTATGATGT CGCCTACCCT GGCCAGCGGA 
GACTTCCCCT TCTCTCCTTG CGACATATCA CCATTCGGGC CCTGCCTCTC CCCGCCACTG 
GACCCACGGG CCCTGCAGTC ACCACCGCTG CGCCCTCCAG ACGTGCCCCC GCCTGAGCAA 
TACTGGAAGG AGGTGGCGGA CCAGAACCAG AGAGCGTTGG GAGACGCGCT TGTTGAGAAT 
AATCAACTGC ACGTGACATT GACCCAGAAA CAGGAGGAGA TCGCCTCGCT CAAGGAGCGG 
AACGTGCAGC TGAAGGAACT CGCCAGCCGA ACCCGGCACC TGGCCTCGGT GCTGGATAAG 
CTGATGATCA CACAGTCCCG GGATTGTGGG GCGGCGGCCG AGCCCTTCCT GCTCAAGGCG 
AAGGCCAAAA GGAGCCTGGA GGAGTTGGTC AGCGCTGCGG GGCAGGATTG CGCGGAAGTG 
GACGCCATCC TGAGGGAGAT TTCCGAGCGC TGCGATGAAG CCCTTCAGAG CCGCGATCCC 
AAGCGGCCCC GACTGCTGCC AGAGCCCGCG AATACTGACA CCAGGCCCGG GAACCTGCAT 
GGCGCCTTCC GGGGGCTGCG CACAGACTGC AGCCGGAGCG CGTTGAACCT GAGCCACAGT 
GAGCTGGAGG AGGGCGGCTC CTTCAGCACC CGCATCCGCA GCCACAGCAC CATCCGCACC 
CTCGCCTTCC CCCAGGGCAA TGCCTTCACC ATCAGAACAG CCAACGGGGG TTACAAGTTC 
CGCTGGGTCC CCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001177716.1
CDS	178..1335
Translation	MQACGGGAAGRRAFDSICPNRMLALPGRALLCKPGKPERKFAPPRKFFPGCTGGSPVSVYEDPPDAEPTALPALTTIDLQDLADCSSLLGSDAPPGGDLAASQNHSHQTEADFNLQDFRDTVDDLISDSSSMMSPTLASGDFPFSPCDISPFGPCLSPPLDPRALQSPPLRPPDVPPPEQYWKEVADQNQRALGDALVENNQLHVTLTQKQEEIASLKERNVQLKELASRTRHLASVLDKLMITQSRDCGAAAEPFLLKAKAKRSLEELVSAAGQDCAEVDAILREISERCDEALQSRDPKRPRLLPEPANTDTRPGNLHGAFRGLRTDCSRSALNLSHSELEEGGSFSTRIRSHSTIRTLAFPQGNAFTIRTANGGYKFRWVPS

Target ORF information:

RefSeq Version	NM_001190787.3
Organism	Homo sapiens(human)
Definition	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001190787.3

ORF Insert Sequence:

ATGCAGGCGT GCGGGGGCGG CGCGGCCGGC CGTCGGGCCT TCGACAGCAT CTGCCCCAAC 
AGAATGCTGG CACTGCCGGG CCGGGCGCTG CTCTGCAAGC CGGGGAAGCC GGAGAGGAAG 
TTCGCTCCTC CGCGGAAGTT CTTCCCCGGA TGCACAGGCG GGAGCCCGGT GTCGGTGTAC 
GAGGATCCCC CGGACGCCGA GCCCACAGCG CTGCCAGCCC TCACCACCAT AGACCTGCAG 
GACCTCGCTG ACTGCTCTTC GCTACTCGGG TCCGACGCGC CGCCTGGTGG TGACCTGGCC 
GCCTCGCAGA ACCATTCCCA CCAAACGGAA GCAGACTTCA ATCTGCAAGA TTTCAGAGAC 
ACGGTGGATG ATCTCATTTC AGACTCATCC TCTATGATGT CGCCTACCCT GGCCAGCGGA 
GACTTCCCCT TCTCTCCTTG CGACATATCA CCATTCGGGC CCTGCCTCTC CCCGCCACTG 
GACCCACGGG CCCTGCAGTC ACCACCGCTG CGCCCTCCAG ACGTGCCCCC GCCTGAGCAA 
TACTGGAAGG AGGTGGCGGA CCAGAACCAG AGAGCGTTGG GAGACGCGCT TGTTGAGAAT 
AATCAACTGC ACGTGACATT GACCCAGAAA CAGGAGGAGA TCGCCTCGCT CAAGGAGCGG 
AACGTGCAGC TGAAGGAACT CGCCAGCCGA ACCCGGCACC TGGCCTCGGT GCTGGATAAG 
CTGATGATCA CACAGTCCCG GGATTGTGGG GCGGCGGCCG AGCCCTTCCT GCTCAAGGCG 
AAGGCCAAAA GGAGCCTGGA GGAGTTGGTC AGCGCTGCGG GGCAGGATTG CGCGGAAGTG 
GACGCCATCC TGAGGGAGAT TTCCGAGCGC TGCGATGAAG CCCTTCAGAG CCGCGATCCC 
AAGCGGCCCC GACTGCTGCC AGAGCCCGCG AATACTGACA CCAGGCCCGG GAACCTGCAT 
GGCGCCTTCC GGGGGCTGCG CACAGACTGC AGCCGGAGCG CGTTGAACCT GAGCCACAGT 
GAGCTGGAGG AGGGCGGCTC CTTCAGCACC CGCATCCGCA GCCACAGCAC CATCCGCACC 
CTCGCCTTCC CCCAGGGCAA TGCCTTCACC ATCAGAACAG CCAACGGGGG TTACAAGTTC 
CGCTGGGTCC CCAGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCIDAS ( XM_017009439.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864928.1 Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), transcript variant X1, mRNA.

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CloneID	OHu95862
Clone ID Related Accession (Same CDS sequence)	XM_017009439.2 , XM_017009439.1
Accession Version	XM_017009439.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	multicilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009439.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGC CTACCCTGGC CAGCGGAGAC TTCCCCTTCT CTCCTTGCGA CATATCACCA 
TTCGGGCCCT GCCTCTCCCC GCCACTGGAC CCACGGGCCC TGCAGTCACC ACCGCTGCGC 
CCTCCAGACG TGCCCCCGCC TGAGCAATAC TGGAAGGAGG TGGCGGACCA GAACCAGAGA 
GCGTTGGGAG ACGCGCTTGT TGAGAATAAT CAACTGCACG TGACATTGAC CCAGAAACAG 
GAGGAGATCG CCTCGCTCAA GGAGCGGAAC GTGCAGCTGA AGGAACTCGC CAGCCGAACC 
CGGCACCTGG CCTCGGTGCT GGATAAGCTG ATGATCACAC AGTCCCGGGA TTGTGGGGCG 
GCGGCCGAGC CCTTCCTGCT CAAGGCGAAG GCCAAAAGGA GCCTGGAGGA GTTGGTCAGC 
GCTGCGGGGC AGGATTGCGC GGAAGTGGAC GCCATCCTGA GGGAGATTTC CGAGCGCTGC 
GATGAAGCCC TTCAGAGCCG CGATCCCAAG CGGCCCCGAC TGCTGCCAGA GCCCGCGAAT 
ACTGACACCA GGCCCGGGAA CCTGCATGGC GCCTTCCGGG GGCTGCGCAC AGACTGCAGC 
CGGAGCGCGT TGAACCTGAG CCACAGTGAG CTGGAGGAGG GCGGCTCCTT CAGCACCCGC 
ATCCGCAGCC ACAGCACCAT CCGCACCCTC GCCTTCCCCC AGGGCAATGC CTTCACCATC 
AGAACAGCCA ACGGGGGTTA CAAGTTCCGC TGGGTCCCCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864928.1
CDS	783..1547
Translation	MMSPTLASGDFPFSPCDISPFGPCLSPPLDPRALQSPPLRPPDVPPPEQYWKEVADQNQRALGDALVENNQLHVTLTQKQEEIASLKERNVQLKELASRTRHLASVLDKLMITQSRDCGAAAEPFLLKAKAKRSLEELVSAAGQDCAEVDAILREISERCDEALQSRDPKRPRLLPEPANTDTRPGNLHGAFRGLRTDCSRSALNLSHSELEEGGSFSTRIRSHSTIRTLAFPQGNAFTIRTANGGYKFRWVPS

Target ORF information:

RefSeq Version	XM_017009439.2
Organism	Homo sapiens(human)
Definition	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009439.2

ORF Insert Sequence:

ATGATGTCGC CTACCCTGGC CAGCGGAGAC TTCCCCTTCT CTCCTTGCGA CATATCACCA 
TTCGGGCCCT GCCTCTCCCC GCCACTGGAC CCACGGGCCC TGCAGTCACC ACCGCTGCGC 
CCTCCAGACG TGCCCCCGCC TGAGCAATAC TGGAAGGAGG TGGCGGACCA GAACCAGAGA 
GCGTTGGGAG ACGCGCTTGT TGAGAATAAT CAACTGCACG TGACATTGAC CCAGAAACAG 
GAGGAGATCG CCTCGCTCAA GGAGCGGAAC GTGCAGCTGA AGGAACTCGC CAGCCGAACC 
CGGCACCTGG CCTCGGTGCT GGATAAGCTG ATGATCACAC AGTCCCGGGA TTGTGGGGCG 
GCGGCCGAGC CCTTCCTGCT CAAGGCGAAG GCCAAAAGGA GCCTGGAGGA GTTGGTCAGC 
GCTGCGGGGC AGGATTGCGC GGAAGTGGAC GCCATCCTGA GGGAGATTTC CGAGCGCTGC 
GATGAAGCCC TTCAGAGCCG CGATCCCAAG CGGCCCCGAC TGCTGCCAGA GCCCGCGAAT 
ACTGACACCA GGCCCGGGAA CCTGCATGGC GCCTTCCGGG GGCTGCGCAC AGACTGCAGC 
CGGAGCGCGT TGAACCTGAG CCACAGTGAG CTGGAGGAGG GCGGCTCCTT CAGCACCCGC 
ATCCGCAGCC ACAGCACCAT CCGCACCCTC GCCTTCCCCC AGGGCAATGC CTTCACCATC 
AGAACAGCCA ACGGGGGTTA CAAGTTCCGC TGGGTCCCCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MCIDAS ( XM_017009439.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016864928.1 Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu95862
Clone ID Related Accession (Same CDS sequence)	XM_017009439.2 , XM_017009439.1
Accession Version	XM_017009439.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	multicilin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGTCGC CTACCCTGGC CAGCGGAGAC TTCCCCTTCT CTCCTTGCGA CATATCACCA 
TTCGGGCCCT GCCTCTCCCC GCCACTGGAC CCACGGGCCC TGCAGTCACC ACCGCTGCGC 
CCTCCAGACG TGCCCCCGCC TGAGCAATAC TGGAAGGAGG TGGCGGACCA GAACCAGAGA 
GCGTTGGGAG ACGCGCTTGT TGAGAATAAT CAACTGCACG TGACATTGAC CCAGAAACAG 
GAGGAGATCG CCTCGCTCAA GGAGCGGAAC GTGCAGCTGA AGGAACTCGC CAGCCGAACC 
CGGCACCTGG CCTCGGTGCT GGATAAGCTG ATGATCACAC AGTCCCGGGA TTGTGGGGCG 
GCGGCCGAGC CCTTCCTGCT CAAGGCGAAG GCCAAAAGGA GCCTGGAGGA GTTGGTCAGC 
GCTGCGGGGC AGGATTGCGC GGAAGTGGAC GCCATCCTGA GGGAGATTTC CGAGCGCTGC 
GATGAAGCCC TTCAGAGCCG CGATCCCAAG CGGCCCCGAC TGCTGCCAGA GCCCGCGAAT 
ACTGACACCA GGCCCGGGAA CCTGCATGGC GCCTTCCGGG GGCTGCGCAC AGACTGCAGC 
CGGAGCGCGT TGAACCTGAG CCACAGTGAG CTGGAGGAGG GCGGCTCCTT CAGCACCCGC 
ATCCGCAGCC ACAGCACCAT CCGCACCCTC GCCTTCCCCC AGGGCAATGC CTTCACCATC 
AGAACAGCCA ACGGGGGTTA CAAGTTCCGC TGGGTCCCCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016864928.1
CDS	783..1547
Translation	MMSPTLASGDFPFSPCDISPFGPCLSPPLDPRALQSPPLRPPDVPPPEQYWKEVADQNQRALGDALVENNQLHVTLTQKQEEIASLKERNVQLKELASRTRHLASVLDKLMITQSRDCGAAAEPFLLKAKAKRSLEELVSAAGQDCAEVDAILREISERCDEALQSRDPKRPRLLPEPANTDTRPGNLHGAFRGLRTDCSRSALNLSHSELEEGGSFSTRIRSHSTIRTLAFPQGNAFTIRTANGGYKFRWVPS

Target ORF information:

RefSeq Version	XM_017009439.1
Organism	Homo sapiens(human)
Definition	Homo sapiens multiciliate differentiation and DNA synthesis associated cell cycle protein (MCIDAS), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009439.1

ORF Insert Sequence:

ATGATGTCGC CTACCCTGGC CAGCGGAGAC TTCCCCTTCT CTCCTTGCGA CATATCACCA 
TTCGGGCCCT GCCTCTCCCC GCCACTGGAC CCACGGGCCC TGCAGTCACC ACCGCTGCGC 
CCTCCAGACG TGCCCCCGCC TGAGCAATAC TGGAAGGAGG TGGCGGACCA GAACCAGAGA 
GCGTTGGGAG ACGCGCTTGT TGAGAATAAT CAACTGCACG TGACATTGAC CCAGAAACAG 
GAGGAGATCG CCTCGCTCAA GGAGCGGAAC GTGCAGCTGA AGGAACTCGC CAGCCGAACC 
CGGCACCTGG CCTCGGTGCT GGATAAGCTG ATGATCACAC AGTCCCGGGA TTGTGGGGCG 
GCGGCCGAGC CCTTCCTGCT CAAGGCGAAG GCCAAAAGGA GCCTGGAGGA GTTGGTCAGC 
GCTGCGGGGC AGGATTGCGC GGAAGTGGAC GCCATCCTGA GGGAGATTTC CGAGCGCTGC 
GATGAAGCCC TTCAGAGCCG CGATCCCAAG CGGCCCCGAC TGCTGCCAGA GCCCGCGAAT 
ACTGACACCA GGCCCGGGAA CCTGCATGGC GCCTTCCGGG GGCTGCGCAC AGACTGCAGC 
CGGAGCGCGT TGAACCTGAG CCACAGTGAG CTGGAGGAGG GCGGCTCCTT CAGCACCCGC 
ATCCGCAGCC ACAGCACCAT CCGCACCCTC GCCTTCCCCC AGGGCAATGC CTTCACCATC 
AGAACAGCCA ACGGGGGTTA CAAGTTCCGC TGGGTCCCCA GTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

MCIDAS mutations result in a mucociliary clearance disorder with reduced generation of multiple motile cilia.
Nature communications54418(2014 Jul)
Boon M,Wallmeier J,Ma L,Loges NT,Jaspers M,Olbrich H,Dougherty GW,Raidt J,Werner C,Amirav I,Hevroni A,Abitbul R,Avital A,Soferman R,Wessels M,O'Callaghan C,Chung EM,Rutman A,Hirst RA,Moya E,Mitchison HM,Van Daele S,De Boeck K,Jorissen M,Kintner C,Cuppens H,Omran H

The Geminin and Idas coiled coils preferentially form a heterodimer that inhibits Geminin function in DNA replication licensing.
The Journal of biological chemistry288(44)31624-34(2013 Nov)
Caillat C,Pefani DE,Gillespie PJ,Taraviras S,Blow JJ,Lygerou Z,Perrakis A

Multicilin promotes centriole assembly and ciliogenesis during multiciliate cell differentiation.
Nature cell biology14(2)140-7(2012 Jan)
Stubbs JL,Vladar EK,Axelrod JD,Kintner C

Idas, a novel phylogenetically conserved geminin-related protein, binds to geminin and is required for cell cycle progression.
The Journal of biological chemistry286(26)23234-46(2011 Jul)
Pefani DE,Dimaki M,Spella M,Karantzelis N,Mitsiki E,Kyrousi C,Symeonidou IE,Perrakis A,Taraviras S,Lygerou Z

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MCIDAS cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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