IDUA cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IDUA
Entrez Gene ID	3425
Full Name	iduronidase, alpha-L-
Synonyms	IDA,MPS1
General protein information	Preferred Names iduronidase, alpha-L- Names alpha-L-iduronidase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 4 Map Location: 4p16.3
Summary	This gene encodes an enzyme that hydrolyzes the terminal alpha-L-iduronic acid residues of two glycosaminoglycans, dermatan sulfate and heparan sulfate. This hydrolysis is required for the lysosomal degradation of these glycosaminoglycans. Mutations in this gene that result in enzymatic deficiency lead to the autosomal recessive disease mucopolysaccharidosis type I (MPS I). [provided by RefSeq, Jul 2008].
Disorder MIM:	252800
Disorder Html:	Mucopolysaccharidosis Ih, 607014 (3); Mucopolysaccharidosis Is, 607016 (3); Mucopolysaccharidosis Ih/s, 607015 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_011513461.2	XP_011511763.1	alpha-L-iduronidase isoform X1
NM_000203.5	NP_000194.2	alpha-L-iduronidase isoform a precursor
NM_001363576.1	NP_001350505.1	alpha-L-iduronidase isoform b
NM_000203.4	NP_000194.2	alpha-L-iduronidase precursor
XM_017008163.1	XP_016863652.1	alpha-L-iduronidase isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	IDUA	XP_003310262.1
Canis lupus familiaris (dog)	FGFRL1	XP_005618736.1
Homo sapiens (human)	IDUA	NP_000194.2
Bos taurus (cattle)	IDUA	XP_002688492.2
Gallus gallus (chicken)	IDUA	NP_001026604.1
Danio rerio (zebrafish)	idua	XP_001923689.3
Mus musculus (house mouse)	Idua	NP_032351.2
Rattus norvegicus (Norway rat)	Idua	NP_001165555.1
Drosophila melanogaster (fruit fly)	CG6201	NP_609489.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010549	XP_314521.4
Xenopus tropicalis (tropical clawed frog)	idua	XP_002935502.2

Gene Ontology
Bibliography

Related articles in PubMed

p.X654R IDUA variant among Thai individuals with intermediate mucopolysaccharidosis type I and its residual activity as demonstrated in COS-7 cells.
Ngiwsara L, Ketudat-Cairns JR, Sawangareetrakul P, Charoenwattanasatien R, Champattanachai V, Kuptanon C, Pangkanon S, Tim-Aroon T, Wattanasirichaigoon D, Svasti J
Annals of human genetics(2017 Dec)

[IDUA gene mutation analysis and prenatal diagnosis of two families affected with mucopolysaccharidosis type I].
Yang X, Mei S, Kong X, Zhao Z, Cai A, Yao J, Li Y, Qin Z
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(3)347-351(2017 Jun)

Neonatal tolerance induction enables accurate evaluation of gene therapy for MPS I in a canine model.
Hinderer C, Bell P, Louboutin JP, Katz N, Zhu Y, Lin G, Choa R, Bagel J, O'Donnell P, Fitzgerald CA, Langan T, Wang P, Casal ML, Haskins ME, Wilson JM
Molecular genetics and metabolism119(1-2)124-30(2016 09)

Report of 5 novel mutations of the ?-L-iduronidase gene and comparison of Korean mutations in relation with those of Japan or China in patients with mucopolysaccharidosis I.
Kwak MJ, Huh R, Kim J, Park HD, Cho SY, Jin DK
BMC medical genetics17(1)58(2016 08)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

IDUA deletion mutation is associate with with mucopolysaccharidosis type I .
Title: [IDUA gene mutation analysis and prenatal diagnosis of two families affected with mucopolysaccharidosis type I].

Using lysosomal storage disease mucopolysaccharidosis type I (MPS I) dogs tolerized to human IDUA as neonates, we evaluated intrathecal delivery of an adeno-associated virus serotype 9 vector expressing human IDUA as a therapy for the central nervous system manifestations of MPS
Title: Neonatal tolerance induction enables accurate evaluation of gene therapy for MPS I in a canine model.

Enzyme activities (acid alpha-glucosidase (GAA), galactocerebrosidase (GALC), glucocerebrosidase (GBA), alpha-galactosidase A (GLA), alpha-iduronidase (IDUA) and sphingomyeline phosphodiesterase-1 (SMPD-1)) were measured on ~43,000 de-identified dried blood spot (DBS) punches, and screen positive samples were submitted for DNA sequencing to obtain genotype confirmation of disease risk
Title: Pilot study of newborn screening for six lysosomal storage diseases using Tandem Mass Spectrometry.

10 unrelated Korean patients with Mucopolysaccharidosis I, p.L346R and c.704ins5 were most commonly found in Korean patients with Mucopolysaccharidosis I.
Title: Report of 5 novel mutations of the ?-L-iduronidase gene and comparison of Korean mutations in relation with those of Japan or China in patients with mucopolysaccharidosis I.

Molecular studies results unveiled the predominance of(Pro533Arg) IDUA variation in a series of 13 Algerian patients with Mucopolysaccharidosis Type I presented mainly with an attenuated phenotype.
Title: Clinical and Molecular Characterization of Patients with Mucopolysaccharidosis Type I in an Algerian Series.

The following IDUA gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDUA cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21821	NM_000203.5 Latest version!	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
OHu38340	NM_001363576.1 Latest version!	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu58358	XM_011513461.2 Latest version!	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu95158	XM_017008163.1 Latest version!	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
View Old Accession Versions >>
OHu21821	NM_000203.4	Homo sapiens iduronidase, alpha-L- (IDUA), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$274.50-$384.30 $549.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IDUA ( NM_000203.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000194.2 Homo sapiens alpha-L-iduronidase (IDUA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21821
Clone ID Related Accession (Same CDS sequence)	NM_000203.5 , NM_000203.4
Accession Version	NM_000203.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	alpha-L-iduronidase isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M74715.1 and AC019103.8. This sequence is a reference standard in the RefSeqGene project. On Aug 20, 2018 this sequence version replaced NM_000203.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M74715.1, AK291816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000514224.2/ ENSP00000425081.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGTCCCC TGCGCCCCCG CGCCGCGCTG CTGGCGCTCC TGGCCTCGCT CCTGGCCGCG 
CCCCCGGTGG CCCCGGCCGA GGCCCCGCAC CTGGTGCATG TGGACGCGGC CCGCGCGCTG 
TGGCCCCTGC GGCGCTTCTG GAGGAGCACA GGCTTCTGCC CCCCGCTGCC ACACAGCCAG 
GCTGACCAGT ACGTCCTCAG CTGGGACCAG CAGCTCAACC TCGCCTATGT GGGCGCCGTC 
CCTCACCGCG GCATCAAGCA GGTCCGGACC CACTGGCTGC TGGAGCTTGT CACCACCAGG 
GGGTCCACTG GACGGGGCCT GAGCTACAAC TTCACCCACC TGGACGGGTA CCTGGACCTT 
CTCAGGGAGA ACCAGCTCCT CCCAGGGTTT GAGCTGATGG GCAGCGCCTC GGGCCACTTC 
ACTGACTTTG AGGACAAGCA GCAGGTGTTT GAGTGGAAGG ACTTGGTCTC CAGCCTGGCC 
AGGAGATACA TCGGTAGGTA CGGACTGGCG CATGTTTCCA AGTGGAACTT CGAGACGTGG 
AATGAGCCAG ACCACCACGA CTTTGACAAC GTCTCCATGA CCATGCAAGG CTTCCTGAAC 
TACTACGATG CCTGCTCGGA GGGTCTGCGC GCCGCCAGCC CCGCCCTGCG GCTGGGAGGC 
CCCGGCGACT CCTTCCACAC CCCACCGCGA TCCCCGCTGA GCTGGGGCCT CCTGCGCCAC 
TGCCACGACG GTACCAACTT CTTCACTGGG GAGGCGGGCG TGCGGCTGGA CTACATCTCC 
CTCCACAGGA AGGGTGCGCG CAGCTCCATC TCCATCCTGG AGCAGGAGAA GGTCGTCGCG 
CAGCAGATCC GGCAGCTCTT CCCCAAGTTC GCGGACACCC CCATTTACAA CGACGAGGCG 
GACCCGCTGG TGGGCTGGTC CCTGCCACAG CCGTGGAGGG CGGACGTGAC CTACGCGGCC 
ATGGTGGTGA AGGTCATCGC GCAGCATCAG AACCTGCTAC TGGCCAACAC CACCTCCGCC 
TTCCCCTACG CGCTCCTGAG CAACGACAAT GCCTTCCTGA GCTACCACCC GCACCCCTTC 
GCGCAGCGCA CGCTCACCGC GCGCTTCCAG GTCAACAACA CCCGCCCGCC GCACGTGCAG 
CTGTTGCGCA AGCCGGTGCT CACGGCCATG GGGCTGCTGG CGCTGCTGGA TGAGGAGCAG 
CTCTGGGCCG AAGTGTCGCA GGCCGGGACC GTCCTGGACA GCAACCACAC GGTGGGCGTC 
CTGGCCAGCG CCCACCGCCC CCAGGGCCCG GCCGACGCCT GGCGCGCCGC GGTGCTGATC 
TACGCGAGCG ACGACACCCG CGCCCACCCC AACCGCAGCG TCGCGGTGAC CCTGCGGCTG 
CGCGGGGTGC CCCCCGGCCC GGGCCTGGTC TACGTCACGC GCTACCTGGA CAACGGGCTC 
TGCAGCCCCG ACGGCGAGTG GCGGCGCCTG GGCCGGCCCG TCTTCCCCAC GGCAGAGCAG 
TTCCGGCGCA TGCGCGCGGC TGAGGACCCG GTGGCCGCGG CGCCCCGCCC CTTACCCGCC 
GGCGGCCGCC TGACCCTGCG CCCCGCGCTG CGGCTGCCGT CGCTTTTGCT GGTGCACGTG 
TGTGCGCGCC CCGAGAAGCC GCCCGGGCAG GTCACGCGGC TCCGCGCCCT GCCCCTGACC 
CAAGGGCAGC TGGTTCTGGT CTGGTCGGAT GAACACGTGG GCTCCAAGTG CCTGTGGACA 
TACGAGATCC AGTTCTCTCA GGACGGTAAG GCGTACACCC CGGTCAGCAG GAAGCCATCG 
ACCTTCAACC TCTTTGTGTT CAGCCCAGAC ACAGGTGCTG TCTCTGGCTC CTACCGAGTT 
CGAGCCCTGG ACTACTGGGC CCGACCAGGC CCCTTCTCGG ACCCTGTGCC GTACCTGGAG 
GTCCCTGTGC CAAGAGGGCC CCCATCCCCG GGCAATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000194.2
CDS	42..2003
Translation	MRPLRPRAALLALLASLLAAPPVAPAEAPHLVHVDAARALWPLRRFWRSTGFCPPLPHSQADQYVLSWDQQLNLAYVGAVPHRGIKQVRTHWLLELVTTRGSTGRGLSYNFTHLDGYLDLLRENQLLPGFELMGSASGHFTDFEDKQQVFEWKDLVSSLARRYIGRYGLAHVSKWNFETWNEPDHHDFDNVSMTMQGFLNYYDACSEGLRAASPALRLGGPGDSFHTPPRSPLSWGLLRHCHDGTNFFTGEAGVRLDYISLHRKGARSSISILEQEKVVAQQIRQLFPKFADTPIYNDEADPLVGWSLPQPWRADVTYAAMVVKVIAQHQNLLLANTTSAFPYALLSNDNAFLSYHPHPFAQRTLTARFQVNNTRPPHVQLLRKPVLTAMGLLALLDEEQLWAEVSQAGTVLDSNHTVGVLASAHRPQGPADAWRAAVLIYASDDTRAHPNRSVAVTLRLRGVPPGPGLVYVTRYLDNGLCSPDGEWRRLGRPVFPTAEQFRRMRAAEDPVAAAPRPLPAGGRLTLRPALRLPSLLLVHVCARPEKPPGQVTRLRALPLTQGQLVLVWSDEHVGSKCLWTYEIQFSQDGKAYTPVSRKPSTFNLFVFSPDTGAVSGSYRVRALDYWARPGPFSDPVPYLEVPVPRGPPSPGNP

Target ORF information:

RefSeq Version	NM_000203.5
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000203.5

ORF Insert Sequence:

ATGCGTCCCC TGCGCCCCCG CGCCGCGCTG CTGGCGCTCC TGGCCTCGCT CCTGGCCGCG 
CCCCCGGTGG CCCCGGCCGA GGCCCCGCAC CTGGTGCATG TGGACGCGGC CCGCGCGCTG 
TGGCCCCTGC GGCGCTTCTG GAGGAGCACA GGCTTCTGCC CCCCGCTGCC ACACAGCCAG 
GCTGACCAGT ACGTCCTCAG CTGGGACCAG CAGCTCAACC TCGCCTATGT GGGCGCCGTC 
CCTCACCGCG GCATCAAGCA GGTCCGGACC CACTGGCTGC TGGAGCTTGT CACCACCAGG 
GGGTCCACTG GACGGGGCCT GAGCTACAAC TTCACCCACC TGGACGGGTA CCTGGACCTT 
CTCAGGGAGA ACCAGCTCCT CCCAGGGTTT GAGCTGATGG GCAGCGCCTC GGGCCACTTC 
ACTGACTTTG AGGACAAGCA GCAGGTGTTT GAGTGGAAGG ACTTGGTCTC CAGCCTGGCC 
AGGAGATACA TCGGTAGGTA CGGACTGGCG CATGTTTCCA AGTGGAACTT CGAGACGTGG 
AATGAGCCAG ACCACCACGA CTTTGACAAC GTCTCCATGA CCATGCAAGG CTTCCTGAAC 
TACTACGATG CCTGCTCGGA GGGTCTGCGC GCCGCCAGCC CCGCCCTGCG GCTGGGAGGC 
CCCGGCGACT CCTTCCACAC CCCACCGCGA TCCCCGCTGA GCTGGGGCCT CCTGCGCCAC 
TGCCACGACG GTACCAACTT CTTCACTGGG GAGGCGGGCG TGCGGCTGGA CTACATCTCC 
CTCCACAGGA AGGGTGCGCG CAGCTCCATC TCCATCCTGG AGCAGGAGAA GGTCGTCGCG 
CAGCAGATCC GGCAGCTCTT CCCCAAGTTC GCGGACACCC CCATTTACAA CGACGAGGCG 
GACCCGCTGG TGGGCTGGTC CCTGCCACAG CCGTGGAGGG CGGACGTGAC CTACGCGGCC 
ATGGTGGTGA AGGTCATCGC GCAGCATCAG AACCTGCTAC TGGCCAACAC CACCTCCGCC 
TTCCCCTACG CGCTCCTGAG CAACGACAAT GCCTTCCTGA GCTACCACCC GCACCCCTTC 
GCGCAGCGCA CGCTCACCGC GCGCTTCCAG GTCAACAACA CCCGCCCGCC GCACGTGCAG 
CTGTTGCGCA AGCCGGTGCT CACGGCCATG GGGCTGCTGG CGCTGCTGGA TGAGGAGCAG 
CTCTGGGCCG AAGTGTCGCA GGCCGGGACC GTCCTGGACA GCAACCACAC GGTGGGCGTC 
CTGGCCAGCG CCCACCGCCC CCAGGGCCCG GCCGACGCCT GGCGCGCCGC GGTGCTGATC 
TACGCGAGCG ACGACACCCG CGCCCACCCC AACCGCAGCG TCGCGGTGAC CCTGCGGCTG 
CGCGGGGTGC CCCCCGGCCC GGGCCTGGTC TACGTCACGC GCTACCTGGA CAACGGGCTC 
TGCAGCCCCG ACGGCGAGTG GCGGCGCCTG GGCCGGCCCG TCTTCCCCAC GGCAGAGCAG 
TTCCGGCGCA TGCGCGCGGC TGAGGACCCG GTGGCCGCGG CGCCCCGCCC CTTACCCGCC 
GGCGGCCGCC TGACCCTGCG CCCCGCGCTG CGGCTGCCGT CGCTTTTGCT GGTGCACGTG 
TGTGCGCGCC CCGAGAAGCC GCCCGGGCAG GTCACGCGGC TCCGCGCCCT GCCCCTGACC 
CAAGGGCAGC TGGTTCTGGT CTGGTCGGAT GAACACGTGG GCTCCAAGTG CCTGTGGACA 
TACGAGATCC AGTTCTCTCA GGACGGTAAG GCGTACACCC CGGTCAGCAG GAAGCCATCG 
ACCTTCAACC TCTTTGTGTT CAGCCCAGAC ACAGGTGCTG TCTCTGGCTC CTACCGAGTT 
CGAGCCCTGG ACTACTGGGC CCGACCAGGC CCCTTCTCGG ACCCTGTGCC GTACCTGGAG 
GTCCCTGTGC CAAGAGGGCC CCCATCCCCG GGCAATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDUA ( NM_000203.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000194.2 Homo sapiens iduronidase, alpha-L- (IDUA), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu21821
Clone ID Related Accession (Same CDS sequence)	NM_000203.5 , NM_000203.4
Accession Version	NM_000203.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1962bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-10
Organism	Homo sapiens(human)
Product	alpha-L-iduronidase precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from M74715.1, DA057148.1, CD367032.1 and AA114859.1. This sequence is a reference standard in the RefSeqGene project. On Jan 15, 2014 this sequence version replaced NM_000203.3. Transcript Variant: This variant (1) encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M74715.1, AK291816.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGTCCCC TGCGCCCCCG CGCCGCGCTG CTGGCGCTCC TGGCCTCGCT CCTGGCCGCG 
CCCCCGGTGG CCCCGGCCGA GGCCCCGCAC CTGGTGCATG TGGACGCGGC CCGCGCGCTG 
TGGCCCCTGC GGCGCTTCTG GAGGAGCACA GGCTTCTGCC CCCCGCTGCC ACACAGCCAG 
GCTGACCAGT ACGTCCTCAG CTGGGACCAG CAGCTCAACC TCGCCTATGT GGGCGCCGTC 
CCTCACCGCG GCATCAAGCA GGTCCGGACC CACTGGCTGC TGGAGCTTGT CACCACCAGG 
GGGTCCACTG GACGGGGCCT GAGCTACAAC TTCACCCACC TGGACGGGTA CCTGGACCTT 
CTCAGGGAGA ACCAGCTCCT CCCAGGGTTT GAGCTGATGG GCAGCGCCTC GGGCCACTTC 
ACTGACTTTG AGGACAAGCA GCAGGTGTTT GAGTGGAAGG ACTTGGTCTC CAGCCTGGCC 
AGGAGATACA TCGGTAGGTA CGGACTGGCG CATGTTTCCA AGTGGAACTT CGAGACGTGG 
AATGAGCCAG ACCACCACGA CTTTGACAAC GTCTCCATGA CCATGCAAGG CTTCCTGAAC 
TACTACGATG CCTGCTCGGA GGGTCTGCGC GCCGCCAGCC CCGCCCTGCG GCTGGGAGGC 
CCCGGCGACT CCTTCCACAC CCCACCGCGA TCCCCGCTGA GCTGGGGCCT CCTGCGCCAC 
TGCCACGACG GTACCAACTT CTTCACTGGG GAGGCGGGCG TGCGGCTGGA CTACATCTCC 
CTCCACAGGA AGGGTGCGCG CAGCTCCATC TCCATCCTGG AGCAGGAGAA GGTCGTCGCG 
CAGCAGATCC GGCAGCTCTT CCCCAAGTTC GCGGACACCC CCATTTACAA CGACGAGGCG 
GACCCGCTGG TGGGCTGGTC CCTGCCACAG CCGTGGAGGG CGGACGTGAC CTACGCGGCC 
ATGGTGGTGA AGGTCATCGC GCAGCATCAG AACCTGCTAC TGGCCAACAC CACCTCCGCC 
TTCCCCTACG CGCTCCTGAG CAACGACAAT GCCTTCCTGA GCTACCACCC GCACCCCTTC 
GCGCAGCGCA CGCTCACCGC GCGCTTCCAG GTCAACAACA CCCGCCCGCC GCACGTGCAG 
CTGTTGCGCA AGCCGGTGCT CACGGCCATG GGGCTGCTGG CGCTGCTGGA TGAGGAGCAG 
CTCTGGGCCG AAGTGTCGCA GGCCGGGACC GTCCTGGACA GCAACCACAC GGTGGGCGTC 
CTGGCCAGCG CCCACCGCCC CCAGGGCCCG GCCGACGCCT GGCGCGCCGC GGTGCTGATC 
TACGCGAGCG ACGACACCCG CGCCCACCCC AACCGCAGCG TCGCGGTGAC CCTGCGGCTG 
CGCGGGGTGC CCCCCGGCCC GGGCCTGGTC TACGTCACGC GCTACCTGGA CAACGGGCTC 
TGCAGCCCCG ACGGCGAGTG GCGGCGCCTG GGCCGGCCCG TCTTCCCCAC GGCAGAGCAG 
TTCCGGCGCA TGCGCGCGGC TGAGGACCCG GTGGCCGCGG CGCCCCGCCC CTTACCCGCC 
GGCGGCCGCC TGACCCTGCG CCCCGCGCTG CGGCTGCCGT CGCTTTTGCT GGTGCACGTG 
TGTGCGCGCC CCGAGAAGCC GCCCGGGCAG GTCACGCGGC TCCGCGCCCT GCCCCTGACC 
CAAGGGCAGC TGGTTCTGGT CTGGTCGGAT GAACACGTGG GCTCCAAGTG CCTGTGGACA 
TACGAGATCC AGTTCTCTCA GGACGGTAAG GCGTACACCC CGGTCAGCAG GAAGCCATCG 
ACCTTCAACC TCTTTGTGTT CAGCCCAGAC ACAGGTGCTG TCTCTGGCTC CTACCGAGTT 
CGAGCCCTGG ACTACTGGGC CCGACCAGGC CCCTTCTCGG ACCCTGTGCC GTACCTGGAG 
GTCCCTGTGC CAAGAGGGCC CCCATCCCCG GGCAATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000194.2
CDS	89..2050
Translation	MRPLRPRAALLALLASLLAAPPVAPAEAPHLVHVDAARALWPLRRFWRSTGFCPPLPHSQADQYVLSWDQQLNLAYVGAVPHRGIKQVRTHWLLELVTTRGSTGRGLSYNFTHLDGYLDLLRENQLLPGFELMGSASGHFTDFEDKQQVFEWKDLVSSLARRYIGRYGLAHVSKWNFETWNEPDHHDFDNVSMTMQGFLNYYDACSEGLRAASPALRLGGPGDSFHTPPRSPLSWGLLRHCHDGTNFFTGEAGVRLDYISLHRKGARSSISILEQEKVVAQQIRQLFPKFADTPIYNDEADPLVGWSLPQPWRADVTYAAMVVKVIAQHQNLLLANTTSAFPYALLSNDNAFLSYHPHPFAQRTLTARFQVNNTRPPHVQLLRKPVLTAMGLLALLDEEQLWAEVSQAGTVLDSNHTVGVLASAHRPQGPADAWRAAVLIYASDDTRAHPNRSVAVTLRLRGVPPGPGLVYVTRYLDNGLCSPDGEWRRLGRPVFPTAEQFRRMRAAEDPVAAAPRPLPAGGRLTLRPALRLPSLLLVHVCARPEKPPGQVTRLRALPLTQGQLVLVWSDEHVGSKCLWTYEIQFSQDGKAYTPVSRKPSTFNLFVFSPDTGAVSGSYRVRALDYWARPGPFSDPVPYLEVPVPRGPPSPGNP

Target ORF information:

RefSeq Version	NM_000203.4
Organism	Homo sapiens(human)
Definition	Homo sapiens iduronidase, alpha-L- (IDUA), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000203.4

ORF Insert Sequence:

ATGCGTCCCC TGCGCCCCCG CGCCGCGCTG CTGGCGCTCC TGGCCTCGCT CCTGGCCGCG 
CCCCCGGTGG CCCCGGCCGA GGCCCCGCAC CTGGTGCATG TGGACGCGGC CCGCGCGCTG 
TGGCCCCTGC GGCGCTTCTG GAGGAGCACA GGCTTCTGCC CCCCGCTGCC ACACAGCCAG 
GCTGACCAGT ACGTCCTCAG CTGGGACCAG CAGCTCAACC TCGCCTATGT GGGCGCCGTC 
CCTCACCGCG GCATCAAGCA GGTCCGGACC CACTGGCTGC TGGAGCTTGT CACCACCAGG 
GGGTCCACTG GACGGGGCCT GAGCTACAAC TTCACCCACC TGGACGGGTA CCTGGACCTT 
CTCAGGGAGA ACCAGCTCCT CCCAGGGTTT GAGCTGATGG GCAGCGCCTC GGGCCACTTC 
ACTGACTTTG AGGACAAGCA GCAGGTGTTT GAGTGGAAGG ACTTGGTCTC CAGCCTGGCC 
AGGAGATACA TCGGTAGGTA CGGACTGGCG CATGTTTCCA AGTGGAACTT CGAGACGTGG 
AATGAGCCAG ACCACCACGA CTTTGACAAC GTCTCCATGA CCATGCAAGG CTTCCTGAAC 
TACTACGATG CCTGCTCGGA GGGTCTGCGC GCCGCCAGCC CCGCCCTGCG GCTGGGAGGC 
CCCGGCGACT CCTTCCACAC CCCACCGCGA TCCCCGCTGA GCTGGGGCCT CCTGCGCCAC 
TGCCACGACG GTACCAACTT CTTCACTGGG GAGGCGGGCG TGCGGCTGGA CTACATCTCC 
CTCCACAGGA AGGGTGCGCG CAGCTCCATC TCCATCCTGG AGCAGGAGAA GGTCGTCGCG 
CAGCAGATCC GGCAGCTCTT CCCCAAGTTC GCGGACACCC CCATTTACAA CGACGAGGCG 
GACCCGCTGG TGGGCTGGTC CCTGCCACAG CCGTGGAGGG CGGACGTGAC CTACGCGGCC 
ATGGTGGTGA AGGTCATCGC GCAGCATCAG AACCTGCTAC TGGCCAACAC CACCTCCGCC 
TTCCCCTACG CGCTCCTGAG CAACGACAAT GCCTTCCTGA GCTACCACCC GCACCCCTTC 
GCGCAGCGCA CGCTCACCGC GCGCTTCCAG GTCAACAACA CCCGCCCGCC GCACGTGCAG 
CTGTTGCGCA AGCCGGTGCT CACGGCCATG GGGCTGCTGG CGCTGCTGGA TGAGGAGCAG 
CTCTGGGCCG AAGTGTCGCA GGCCGGGACC GTCCTGGACA GCAACCACAC GGTGGGCGTC 
CTGGCCAGCG CCCACCGCCC CCAGGGCCCG GCCGACGCCT GGCGCGCCGC GGTGCTGATC 
TACGCGAGCG ACGACACCCG CGCCCACCCC AACCGCAGCG TCGCGGTGAC CCTGCGGCTG 
CGCGGGGTGC CCCCCGGCCC GGGCCTGGTC TACGTCACGC GCTACCTGGA CAACGGGCTC 
TGCAGCCCCG ACGGCGAGTG GCGGCGCCTG GGCCGGCCCG TCTTCCCCAC GGCAGAGCAG 
TTCCGGCGCA TGCGCGCGGC TGAGGACCCG GTGGCCGCGG CGCCCCGCCC CTTACCCGCC 
GGCGGCCGCC TGACCCTGCG CCCCGCGCTG CGGCTGCCGT CGCTTTTGCT GGTGCACGTG 
TGTGCGCGCC CCGAGAAGCC GCCCGGGCAG GTCACGCGGC TCCGCGCCCT GCCCCTGACC 
CAAGGGCAGC TGGTTCTGGT CTGGTCGGAT GAACACGTGG GCTCCAAGTG CCTGTGGACA 
TACGAGATCC AGTTCTCTCA GGACGGTAAG GCGTACACCC CGGTCAGCAG GAAGCCATCG 
ACCTTCAACC TCTTTGTGTT CAGCCCAGAC ACAGGTGCTG TCTCTGGCTC CTACCGAGTT 
CGAGCCCTGG ACTACTGGGC CCGACCAGGC CCCTTCTCGG ACCCTGTGCC GTACCTGGAG 
GTCCCTGTGC CAAGAGGGCC CCCATCCCCG GGCAATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDUA ( NM_001363576.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350505.1 Homo sapiens alpha-L-iduronidase (IDUA), transcript variant 3, mRNA.

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CloneID	OHu38340
Clone ID Related Accession (Same CDS sequence)	NM_001363576.1
Accession Version	NM_001363576.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1566bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	alpha-L-iduronidase isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK098805.1 and AC019103.8. On May 24, 2018 this sequence version replaced XM_024454029.1. Transcript Variant: This variant (3) differs in the 5' UTR and coding sequence compared to variant 1. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK098805.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2142348 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCAGCG CCTCGGGCCA CTTCACTGAC TTTGAGGACA AGCAGCAGGT GTTTGAGTGG 
AAGGACTTGG TCTCCAGCCT GGCCAGGAGA TACATCGGTA GGTACGGACT GGCGCATGTT 
TCCAAGTGGA ACTTCGAGAC GTGGAATGAG CCAGACCACC ACGACTTTGA CAACGTCTCC 
ATGACCATGC AAGGCTTCCT GAACTACTAC GATGCCTGCT CGGAGGGTCT GCGCGCCGCC 
AGCCCCGCCC TGCGGCTGGG AGGCCCCGGC GACTCCTTCC ACACCCCACC GCGATCCCCG 
CTGAGCTGGG GCCTCCTGCG CCACTGCCAC GACGGTACCA ACTTCTTCAC TGGGGAGGCG 
GGCGTGCGGC TGGACTACAT CTCCCTCCAC AGGAAGGGTG CGCGCAGCTC CATCTCCATC 
CTGGAGCAGG AGAAGGTCGT CGCGCAGCAG ATCCGGCAGC TCTTCCCCAA GTTCGCGGAC 
ACCCCCATTT ACAACGACGA GGCGGACCCG CTGGTGGGCT GGTCCCTGCC ACAGCCGTGG 
AGGGCGGACG TGACCTACGC GGCCATGGTG GTGAAGGTCA TCGCGCAGCA TCAGAACCTG 
CTACTGGCCA ACACCACCTC CGCCTTCCCC TACGCGCTCC TGAGCAACGA CAATGCCTTC 
CTGAGCTACC ACCCGCACCC CTTCGCGCAG CGCACGCTCA CCGCGCGCTT CCAGGTCAAC 
AACACCCGCC CGCCGCACGT GCAGCTGTTG CGCAAGCCGG TGCTCACGGC CATGGGGCTG 
CTGGCGCTGC TGGATGAGGA GCAGCTCTGG GCCGAAGTGT CGCAGGCCGG GACCGTCCTG 
GACAGCAACC ACACGGTGGG CGTCCTGGCC AGCGCCCACC GCCCCCAGGG CCCGGCCGAC 
GCCTGGCGCG CCGCGGTGCT GATCTACGCG AGCGACGACA CCCGCGCCCA CCCCAACCGC 
AGCGTCGCGG TGACCCTGCG GCTGCGCGGG GTGCCCCCCG GCCCGGGCCT GGTCTACGTC 
ACGCGCTACC TGGACAACGG GCTCTGCAGC CCCGACGGCG AGTGGCGGCG CCTGGGCCGG 
CCCGTCTTCC CCACGGCAGA GCAGTTCCGG CGCATGCGCG CGGCTGAGGA CCCGGTGGCC 
GCGGCGCCCC GCCCCTTACC CGCCGGCGGC CGCCTGACCC TGCGCCCCGC GCTGCGGCTG 
CCGTCGCTTT TGCTGGTGCA CGTGTGTGCG CGCCCCGAGA AGCCGCCCGG GCAGGTCACG 
CGGCTCCGCG CCCTGCCCCT GACCCAAGGG CAGCTGGTTC TGGTCTGGTC GGATGAACAC 
GTGGGCTCCA AGTGCCTGTG GACATACGAG ATCCAGTTCT CTCAGGACGG TAAGGCGTAC 
ACCCCGGTCA GCAGGAAGCC ATCGACCTTC AACCTCTTTG TGTTCAGCCC AGACACAGGT 
GCTGTCTCTG GCTCCTACCG AGTTCGAGCC CTGGACTACT GGGCCCGACC AGGCCCCTTC 
TCGGACCCTG TGCCGTACCT GGAGGTCCCT GTGCCAAGAG GGCCCCCATC CCCGGGCAAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350505.1
CDS	453..2018
Translation	MGSASGHFTDFEDKQQVFEWKDLVSSLARRYIGRYGLAHVSKWNFETWNEPDHHDFDNVSMTMQGFLNYYDACSEGLRAASPALRLGGPGDSFHTPPRSPLSWGLLRHCHDGTNFFTGEAGVRLDYISLHRKGARSSISILEQEKVVAQQIRQLFPKFADTPIYNDEADPLVGWSLPQPWRADVTYAAMVVKVIAQHQNLLLANTTSAFPYALLSNDNAFLSYHPHPFAQRTLTARFQVNNTRPPHVQLLRKPVLTAMGLLALLDEEQLWAEVSQAGTVLDSNHTVGVLASAHRPQGPADAWRAAVLIYASDDTRAHPNRSVAVTLRLRGVPPGPGLVYVTRYLDNGLCSPDGEWRRLGRPVFPTAEQFRRMRAAEDPVAAAPRPLPAGGRLTLRPALRLPSLLLVHVCARPEKPPGQVTRLRALPLTQGQLVLVWSDEHVGSKCLWTYEIQFSQDGKAYTPVSRKPSTFNLFVFSPDTGAVSGSYRVRALDYWARPGPFSDPVPYLEVPVPRGPPSPGNP

Target ORF information:

RefSeq Version	NM_001363576.1
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363576.1

ORF Insert Sequence:

ATGGGCAGCG CCTCGGGCCA CTTCACTGAC TTTGAGGACA AGCAGCAGGT GTTTGAGTGG 
AAGGACTTGG TCTCCAGCCT GGCCAGGAGA TACATCGGTA GGTACGGACT GGCGCATGTT 
TCCAAGTGGA ACTTCGAGAC GTGGAATGAG CCAGACCACC ACGACTTTGA CAACGTCTCC 
ATGACCATGC AAGGCTTCCT GAACTACTAC GATGCCTGCT CGGAGGGTCT GCGCGCCGCC 
AGCCCCGCCC TGCGGCTGGG AGGCCCCGGC GACTCCTTCC ACACCCCACC GCGATCCCCG 
CTGAGCTGGG GCCTCCTGCG CCACTGCCAC GACGGTACCA ACTTCTTCAC TGGGGAGGCG 
GGCGTGCGGC TGGACTACAT CTCCCTCCAC AGGAAGGGTG CGCGCAGCTC CATCTCCATC 
CTGGAGCAGG AGAAGGTCGT CGCGCAGCAG ATCCGGCAGC TCTTCCCCAA GTTCGCGGAC 
ACCCCCATTT ACAACGACGA GGCGGACCCG CTGGTGGGCT GGTCCCTGCC ACAGCCGTGG 
AGGGCGGACG TGACCTACGC GGCCATGGTG GTGAAGGTCA TCGCGCAGCA TCAGAACCTG 
CTACTGGCCA ACACCACCTC CGCCTTCCCC TACGCGCTCC TGAGCAACGA CAATGCCTTC 
CTGAGCTACC ACCCGCACCC CTTCGCGCAG CGCACGCTCA CCGCGCGCTT CCAGGTCAAC 
AACACCCGCC CGCCGCACGT GCAGCTGTTG CGCAAGCCGG TGCTCACGGC CATGGGGCTG 
CTGGCGCTGC TGGATGAGGA GCAGCTCTGG GCCGAAGTGT CGCAGGCCGG GACCGTCCTG 
GACAGCAACC ACACGGTGGG CGTCCTGGCC AGCGCCCACC GCCCCCAGGG CCCGGCCGAC 
GCCTGGCGCG CCGCGGTGCT GATCTACGCG AGCGACGACA CCCGCGCCCA CCCCAACCGC 
AGCGTCGCGG TGACCCTGCG GCTGCGCGGG GTGCCCCCCG GCCCGGGCCT GGTCTACGTC 
ACGCGCTACC TGGACAACGG GCTCTGCAGC CCCGACGGCG AGTGGCGGCG CCTGGGCCGG 
CCCGTCTTCC CCACGGCAGA GCAGTTCCGG CGCATGCGCG CGGCTGAGGA CCCGGTGGCC 
GCGGCGCCCC GCCCCTTACC CGCCGGCGGC CGCCTGACCC TGCGCCCCGC GCTGCGGCTG 
CCGTCGCTTT TGCTGGTGCA CGTGTGTGCG CGCCCCGAGA AGCCGCCCGG GCAGGTCACG 
CGGCTCCGCG CCCTGCCCCT GACCCAAGGG CAGCTGGTTC TGGTCTGGTC GGATGAACAC 
GTGGGCTCCA AGTGCCTGTG GACATACGAG ATCCAGTTCT CTCAGGACGG TAAGGCGTAC 
ACCCCGGTCA GCAGGAAGCC ATCGACCTTC AACCTCTTTG TGTTCAGCCC AGACACAGGT 
GCTGTCTCTG GCTCCTACCG AGTTCGAGCC CTGGACTACT GGGCCCGACC AGGCCCCTTC 
TCGGACCCTG TGCCGTACCT GGAGGTCCCT GTGCCAAGAG GGCCCCCATC CCCGGGCAAT 
CCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDUA ( XM_011513461.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011511763.1 Homo sapiens alpha-L-iduronidase (IDUA), transcript variant X2, mRNA.

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CloneID	OHu58358
Clone ID Related Accession (Same CDS sequence)	XM_011513461.2
Accession Version	XM_011513461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-L-iduronidase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011513461.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGCCTAC CGTCAGAATG TCACAGTCAC TGGACACAGG TGCAGGCAAG CCTGAGTCCA 
GCTGCTCCGT GTCCACCTCC CTGGCTCCCC AGGGGGTCCA CTGGACGGGG CCTGAGCTAC 
AACTTCACCC ACCTGGACGG GTACCTGGAC CTTCTCAGGG AGAACCAGCT CCTCCCAGGG 
TTTGAGCTGA TGGGCAGCGC CTCGGGCCAC TTCACTGACT TTGAGGACAA GCAGCAGGTG 
TTTGAGTGGA AGGACTTGGT CTCCAGCCTG GCCAGGAGAT ACATCGGTAG GTACGGACTG 
GCGCATGTTT CCAAGTGGAA CTTCGAGACG TGGAATGAGC CAGACCACCA CGACTTTGAC 
AACGTCTCCA TGACCATGCA AGGCTTCCTG AACTACTACG ATGCCTGCTC GGAGGGTCTG 
CGCGCCGCCA GCCCCGCCCT GCGGCTGGGA GGCCCCGGCG ACTCCTTCCA CACCCCACCG 
CGATCCCCGC TGAGCTGGGG CCTCCTGCGC CACTGCCACG ACGGTACCAA CTTCTTCACT 
GGGGAGGCGG GCGTGCGGCT GGACTACATC TCCCTCCACA GGAAGGGTGC GCGCAGCTCC 
ATCTCCATCC TGGAGCAGGA GAAGGTCGTC GCGCAGCAGA TCCGGCAGCT CTTCCCCAAG 
TTCGCGGACA CCCCCATTTA CAACGACGAG GCGGACCCGC TGGTGGGCTG GTCCCTGCCA 
CAGCCGTGGA GGGCGGACGT GACCTACGCG GCCATGGTGG TGAAGGTCAT CGCGCAGCAT 
CAGAACCTGC TACTGGCCAA CACCACCTCC GCCTTCCCCT ACGCGCTCCT GAGCAACGAC 
AATGCCTTCC TGAGCTACCA CCCGCACCCC TTCGCGCAGC GCACGCTCAC CGCGCGCTTC 
CAGGTCAACA ACACCCGCCC GCCGCACGTG CAGCTGTTGC GCAAGCCGGT GCTCACGGCC 
ATGGGGCTGC TGGCGCTGCT GGATGAGGAG CAGCTCTGGG CCGAAGTGTC GCAGGCCGGG 
ACCGTCCTGG ACAGCAACCA CACGGTGGGC GTCCTGGCCA GCGCCCACCG CCCCCAGGGC 
CCGGCCGACG CCTGGCGCGC CGCGGTGCTG ATCTACGCGA GCGACGACAC CCGCGCCCAC 
CCCAACCGCA GCGTCGCGGT GACCCTGCGG CTGCGCGGGG TGCCCCCCGG CCCGGGCCTG 
GTCTACGTCA CGCGCTACCT GGACAACGGG CTCTGCAGCC CCGACGGCGA GTGGCGGCGC 
CTGGGCCGGC CCGTCTTCCC CACGGCAGAG CAGTTCCGGC GCATGCGCGC GGCTGAGGAC 
CCGGTGGCCG CGGCGCCCCG CCCCTTACCC GCCGGCGGCC GCCTGACCCT GCGCCCCGCG 
CTGCGGCTGC CGTCGCTTTT GCTGGTGCAC GTGTGTGCGC GCCCCGAGAA GCCGCCCGGG 
CAGGTCACGC GGCTCCGCGC CCTGCCCCTG ACCCAAGGGC AGCTGGTTCT GGTCTGGTCG 
GATGAACACG TGGGCTCCAA GTGCCTGTGG ACATACGAGA TCCAGTTCTC TCAGGACGGT 
AAGGCGTACA CCCCGGTCAG CAGGAAGCCA TCGACCTTCA ACCTCTTTGT GTTCAGCCCA 
GACACAGGTG CTGTCTCTGG CTCCTACCGA GTTCGAGCCC TGGACTACTG GGCCCGACCA 
GGCCCCTTCT CGGACCCTGT GCCGTACCTG GAGGTCCCTG TGCCAAGAGG GCCCCCATCC 
CCGGGCAATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011511763.1
CDS	441..2195
Translation	MCLPSECHSHWTQVQASLSPAAPCPPPWLPRGSTGRGLSYNFTHLDGYLDLLRENQLLPGFELMGSASGHFTDFEDKQQVFEWKDLVSSLARRYIGRYGLAHVSKWNFETWNEPDHHDFDNVSMTMQGFLNYYDACSEGLRAASPALRLGGPGDSFHTPPRSPLSWGLLRHCHDGTNFFTGEAGVRLDYISLHRKGARSSISILEQEKVVAQQIRQLFPKFADTPIYNDEADPLVGWSLPQPWRADVTYAAMVVKVIAQHQNLLLANTTSAFPYALLSNDNAFLSYHPHPFAQRTLTARFQVNNTRPPHVQLLRKPVLTAMGLLALLDEEQLWAEVSQAGTVLDSNHTVGVLASAHRPQGPADAWRAAVLIYASDDTRAHPNRSVAVTLRLRGVPPGPGLVYVTRYLDNGLCSPDGEWRRLGRPVFPTAEQFRRMRAAEDPVAAAPRPLPAGGRLTLRPALRLPSLLLVHVCARPEKPPGQVTRLRALPLTQGQLVLVWSDEHVGSKCLWTYEIQFSQDGKAYTPVSRKPSTFNLFVFSPDTGAVSGSYRVRALDYWARPGPFSDPVPYLEVPVPRGPPSPGNP

Target ORF information:

RefSeq Version	XM_011513461.2
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011513461.2

ORF Insert Sequence:

ATGTGCCTAC CGTCAGAATG TCACAGTCAC TGGACACAGG TGCAGGCAAG CCTGAGTCCA 
GCTGCTCCGT GTCCACCTCC CTGGCTCCCC AGGGGGTCCA CTGGACGGGG CCTGAGCTAC 
AACTTCACCC ACCTGGACGG GTACCTGGAC CTTCTCAGGG AGAACCAGCT CCTCCCAGGG 
TTTGAGCTGA TGGGCAGCGC CTCGGGCCAC TTCACTGACT TTGAGGACAA GCAGCAGGTG 
TTTGAGTGGA AGGACTTGGT CTCCAGCCTG GCCAGGAGAT ACATCGGTAG GTACGGACTG 
GCGCATGTTT CCAAGTGGAA CTTCGAGACG TGGAATGAGC CAGACCACCA CGACTTTGAC 
AACGTCTCCA TGACCATGCA AGGCTTCCTG AACTACTACG ATGCCTGCTC GGAGGGTCTG 
CGCGCCGCCA GCCCCGCCCT GCGGCTGGGA GGCCCCGGCG ACTCCTTCCA CACCCCACCG 
CGATCCCCGC TGAGCTGGGG CCTCCTGCGC CACTGCCACG ACGGTACCAA CTTCTTCACT 
GGGGAGGCGG GCGTGCGGCT GGACTACATC TCCCTCCACA GGAAGGGTGC GCGCAGCTCC 
ATCTCCATCC TGGAGCAGGA GAAGGTCGTC GCGCAGCAGA TCCGGCAGCT CTTCCCCAAG 
TTCGCGGACA CCCCCATTTA CAACGACGAG GCGGACCCGC TGGTGGGCTG GTCCCTGCCA 
CAGCCGTGGA GGGCGGACGT GACCTACGCG GCCATGGTGG TGAAGGTCAT CGCGCAGCAT 
CAGAACCTGC TACTGGCCAA CACCACCTCC GCCTTCCCCT ACGCGCTCCT GAGCAACGAC 
AATGCCTTCC TGAGCTACCA CCCGCACCCC TTCGCGCAGC GCACGCTCAC CGCGCGCTTC 
CAGGTCAACA ACACCCGCCC GCCGCACGTG CAGCTGTTGC GCAAGCCGGT GCTCACGGCC 
ATGGGGCTGC TGGCGCTGCT GGATGAGGAG CAGCTCTGGG CCGAAGTGTC GCAGGCCGGG 
ACCGTCCTGG ACAGCAACCA CACGGTGGGC GTCCTGGCCA GCGCCCACCG CCCCCAGGGC 
CCGGCCGACG CCTGGCGCGC CGCGGTGCTG ATCTACGCGA GCGACGACAC CCGCGCCCAC 
CCCAACCGCA GCGTCGCGGT GACCCTGCGG CTGCGCGGGG TGCCCCCCGG CCCGGGCCTG 
GTCTACGTCA CGCGCTACCT GGACAACGGG CTCTGCAGCC CCGACGGCGA GTGGCGGCGC 
CTGGGCCGGC CCGTCTTCCC CACGGCAGAG CAGTTCCGGC GCATGCGCGC GGCTGAGGAC 
CCGGTGGCCG CGGCGCCCCG CCCCTTACCC GCCGGCGGCC GCCTGACCCT GCGCCCCGCG 
CTGCGGCTGC CGTCGCTTTT GCTGGTGCAC GTGTGTGCGC GCCCCGAGAA GCCGCCCGGG 
CAGGTCACGC GGCTCCGCGC CCTGCCCCTG ACCCAAGGGC AGCTGGTTCT GGTCTGGTCG 
GATGAACACG TGGGCTCCAA GTGCCTGTGG ACATACGAGA TCCAGTTCTC TCAGGACGGT 
AAGGCGTACA CCCCGGTCAG CAGGAAGCCA TCGACCTTCA ACCTCTTTGT GTTCAGCCCA 
GACACAGGTG CTGTCTCTGG CTCCTACCGA GTTCGAGCCC TGGACTACTG GGCCCGACCA 
GGCCCCTTCT CGGACCCTGT GCCGTACCTG GAGGTCCCTG TGCCAAGAGG GCCCCCATCC 
CCGGGCAATC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDUA ( XM_017008163.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016863652.1 Homo sapiens alpha-L-iduronidase (IDUA), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu95158
Clone ID Related Accession (Same CDS sequence)	XM_017008163.1
Accession Version	XM_017008163.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1002bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	alpha-L-iduronidase isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000004.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTGA AGGTCATCGC GCAGCATCAG AACCTGCTAC TGGCCAACAC CACCTCCGCC 
TTCCCCTACG CGCTCCTGAG CAACGACAAT GCCTTCCTGA GCTACCACCC GCACCCCTTC 
GCGCAGCGCA CGCTCACCGC GCGCTTCCAG GTCAACAACA CCCGCCCGCC GCACGTGCAG 
CTGTTGCGCA AGCCGGTGCT CACGGCCATG GGGCTGCTGG CGCTGCTGGA TGAGGAGCAG 
CTCTGGGCCG AAGTGTCGCA GGCCGGGACC GTCCTGGACA GCAACCACAC GGTGGGCGTC 
CTGGCCAGCG CCCACCGCCC CCAGGGCCCG GCCGACGCCT GGCGCGCCGC GGTGCTGATC 
TACGCGAGCG ACGACACCCG CGCCCACCCC AACCGCAGCG TCGCGGTGAC CCTGCGGCTG 
CGCGGGGTGC CCCCCGGCCC GGGCCTGGTC TACGTCACGC GCTACCTGGA CAACGGGCTC 
TGCAGCCCCG ACGGCGAGTG GCGGCGCCTG GGCCGGCCCG TCTTCCCCAC GGCAGAGCAG 
TTCCGGCGCA TGCGCGCGGC TGAGGACCCG GTGGCCGCGG CGCCCCGCCC CTTACCCGCC 
GGCGGCCGCC TGACCCTGCG CCCCGCGCTG CGGCTGCCGT CGCTTTTGCT GGTGCACGTG 
TGTGCGCGCC CCGAGAAGCC GCCCGGGCAG GTCACGCGGC TCCGCGCCCT GCCCCTGACC 
CAAGGGCAGC TGGTTCTGGT CTGGTCGGAT GAACACGTGG GCTCCAAGTG CCTGTGGACA 
TACGAGATCC AGTTCTCTCA GGACGGTAAG GCGTACACCC CGGTCAGCAG GAAGCCATCG 
ACCTTCAACC TCTTTGTGTT CAGCCCAGAC ACAGGTGCTG TCTCTGGCTC CTACCGAGTT 
CGAGCCCTGG ACTACTGGGC CCGACCAGGC CCCTTCTCGG ACCCTGTGCC GTACCTGGAG 
GTCCCTGTGC CAAGAGGGCC CCCATCCCCG GGCAATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016863652.1
CDS	1503..2504
Translation	MVVKVIAQHQNLLLANTTSAFPYALLSNDNAFLSYHPHPFAQRTLTARFQVNNTRPPHVQLLRKPVLTAMGLLALLDEEQLWAEVSQAGTVLDSNHTVGVLASAHRPQGPADAWRAAVLIYASDDTRAHPNRSVAVTLRLRGVPPGPGLVYVTRYLDNGLCSPDGEWRRLGRPVFPTAEQFRRMRAAEDPVAAAPRPLPAGGRLTLRPALRLPSLLLVHVCARPEKPPGQVTRLRALPLTQGQLVLVWSDEHVGSKCLWTYEIQFSQDGKAYTPVSRKPSTFNLFVFSPDTGAVSGSYRVRALDYWARPGPFSDPVPYLEVPVPRGPPSPGNP

Target ORF information:

RefSeq Version	XM_017008163.1
Organism	Homo sapiens(human)
Definition	Homo sapiens alpha-L-iduronidase (IDUA), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017008163.1

ORF Insert Sequence:

ATGGTGGTGA AGGTCATCGC GCAGCATCAG AACCTGCTAC TGGCCAACAC CACCTCCGCC 
TTCCCCTACG CGCTCCTGAG CAACGACAAT GCCTTCCTGA GCTACCACCC GCACCCCTTC 
GCGCAGCGCA CGCTCACCGC GCGCTTCCAG GTCAACAACA CCCGCCCGCC GCACGTGCAG 
CTGTTGCGCA AGCCGGTGCT CACGGCCATG GGGCTGCTGG CGCTGCTGGA TGAGGAGCAG 
CTCTGGGCCG AAGTGTCGCA GGCCGGGACC GTCCTGGACA GCAACCACAC GGTGGGCGTC 
CTGGCCAGCG CCCACCGCCC CCAGGGCCCG GCCGACGCCT GGCGCGCCGC GGTGCTGATC 
TACGCGAGCG ACGACACCCG CGCCCACCCC AACCGCAGCG TCGCGGTGAC CCTGCGGCTG 
CGCGGGGTGC CCCCCGGCCC GGGCCTGGTC TACGTCACGC GCTACCTGGA CAACGGGCTC 
TGCAGCCCCG ACGGCGAGTG GCGGCGCCTG GGCCGGCCCG TCTTCCCCAC GGCAGAGCAG 
TTCCGGCGCA TGCGCGCGGC TGAGGACCCG GTGGCCGCGG CGCCCCGCCC CTTACCCGCC 
GGCGGCCGCC TGACCCTGCG CCCCGCGCTG CGGCTGCCGT CGCTTTTGCT GGTGCACGTG 
TGTGCGCGCC CCGAGAAGCC GCCCGGGCAG GTCACGCGGC TCCGCGCCCT GCCCCTGACC 
CAAGGGCAGC TGGTTCTGGT CTGGTCGGAT GAACACGTGG GCTCCAAGTG CCTGTGGACA 
TACGAGATCC AGTTCTCTCA GGACGGTAAG GCGTACACCC CGGTCAGCAG GAAGCCATCG 
ACCTTCAACC TCTTTGTGTT CAGCCCAGAC ACAGGTGCTG TCTCTGGCTC CTACCGAGTT 
CGAGCCCTGG ACTACTGGGC CCGACCAGGC CCCTTCTCGG ACCCTGTGCC GTACCTGGAG 
GTCCCTGTGC CAAGAGGGCC CCCATCCCCG GGCAATCCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

p.X654R IDUA variant among Thai individuals with intermediate mucopolysaccharidosis type I and its residual activity as demonstrated in COS-7 cells.
Annals of human genetics(2017 Dec)
Ngiwsara L,Ketudat-Cairns JR,Sawangareetrakul P,Charoenwattanasatien R,Champattanachai V,Kuptanon C,Pangkanon S,Tim-Aroon T,Wattanasirichaigoon D,Svasti J

[IDUA gene mutation analysis and prenatal diagnosis of two families affected with mucopolysaccharidosis type I].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics34(3)347-351(2017 Jun)
Yang X,Mei S,Kong X,Zhao Z,Cai A,Yao J,Li Y,Qin Z

Neonatal tolerance induction enables accurate evaluation of gene therapy for MPS I in a canine model.
Molecular genetics and metabolism119(1-2)124-30(2016 09)
Hinderer C,Bell P,Louboutin JP,Katz N,Zhu Y,Lin G,Choa R,Bagel J,O'Donnell P,Fitzgerald CA,Langan T,Wang P,Casal ML,Haskins ME,Wilson JM

Report of 5 novel mutations of the ?-L-iduronidase gene and comparison of Korean mutations in relation with those of Japan or China in patients with mucopolysaccharidosis I.
BMC medical genetics17(1)58(2016 08)
Kwak MJ,Huh R,Kim J,Park HD,Cho SY,Jin DK

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IDUA cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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