IDH3G cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	IDH3G
Entrez Gene ID	3421
Full Name	isocitrate dehydrogenase 3 (NAD(+)) gamma
Synonyms	H-IDHG
General protein information	Preferred Names isocitrate dehydrogenase 3 (NAD(+)) gamma Names isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial IDH-gamma NAD (H)-specific isocitrate dehydrogenase gamma subunit NAD(+)-specific ICDH subunit gamma NAD+-specific ICDH isocitrate dehydrogenase 3 (NAD+) gamma isocitrate dehydrogenase 3 gamma isocitrate dehydrogenase, NAD(+)-specific, mitochondrial, gamma subunit isocitric dehydrogenase subunit gamma
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq28
Summary	Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the gamma subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. This gene is a candidate gene for periventricular heterotopia. Several alternatively spliced transcript variants of this gene have been described, but only some of their full length natures have been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	300089

mRNA and Protein(s)

mRNA	Protein	Name
NM_004135.4	NP_004126.1	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform a precursor
NM_174869.3	NP_777358.1	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform b precursor
NM_004135.3	NP_004126.1	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform a precursor
NM_174869.2	NP_777358.1	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform b precursor

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002192	XP_003436054.1
Canis lupus familiaris (dog)	IDH3G	XP_538201.2
Mus musculus (house mouse)	Idh3g	NP_032349.1
Danio rerio (zebrafish)	zgc:113076	NP_001230101.1
Arabidopsis thaliana (thale cress)	IDH-III	NP_195290.1
Pan troglodytes (chimpanzee)	IDH3G	XP_003954161.1
Bos taurus (cattle)	IDH3G	NP_001069781.1
Homo sapiens (human)	IDH3G	NP_004126.1
Caenorhabditis elegans (roundworm)	idhg-1	NP_497927.2
Xenopus tropicalis (tropical clawed frog)	idh3g	NP_001025597.1
Rattus norvegicus (Norway rat)	Idh3g	NP_113739.1
Drosophila melanogaster (fruit fly)	CG5028	NP_001097923.1
Caenorhabditis elegans (roundworm)	idhg-2	NP_491989.1

Gene Ontology
Bibliography

Related articles in PubMed

Molecular mechanism of the allosteric regulation of the ?? heterodimer of human NAD-dependent isocitrate dehydrogenase.
Ma T, Peng Y, Huang W, Ding J
Scientific reports740921(2017 Jan)

Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase.
Bzymek KP, Colman RF
Biochemistry46(18)5391-7(2007 May)

Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase.
Soundar S, O'hagan M, Fomulu KS, Colman RF
The Journal of biological chemistry281(30)21073-81(2006 Jul)

Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase.
Soundar S, Park JH, Huh TL, Colman RF
The Journal of biological chemistry278(52)52146-53(2003 Dec)

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
Simpson JC, Wellenreuther R, Poustka A, Pepperkok R, Wiemann S
EMBO reports1(3)287-92(2000 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Aspartate-190 is a determinant of IDH gamma subunit affinity for the manganese (MnII) ion, as well as for nicotinamide-adenine dinucleotide (NAD), but is not directly required for the catalytic reaction.
Title: Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase.

active sites of the human NAD-IDH are shared between alpha and gamma subunits and between alpha and beta subunits
Title: Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase.

The following IDH3G gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IDH3G cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10112	NM_004135.4 Latest version!	Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma (IDH3G), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu10116	NM_174869.3 Latest version!	Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma (IDH3G), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu10112	NM_004135.3	Homo sapiens isocitrate dehydrogenase 3 (NAD(+)) gamma (IDH3G), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu10116	NM_174869.2	Homo sapiens isocitrate dehydrogenase 3 (NAD(+)) gamma (IDH3G), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IDH3G ( NM_004135.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_004126.1 Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma (IDH3G), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10112
Clone ID Related Accession (Same CDS sequence)	NM_004135.4 , NM_004135.3
Accession Version	NM_004135.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI831096.1 and BC000933.2. On Nov 22, 2018 this sequence version replaced NM_004135.3. Transcript Variant: This variant (1) encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: Z68907.1, SRR3476690.946117.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000217901.10/ ENSP00000217901.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
ATGCACACTC CGGACATCGG GGGCCAGGGC ACAACATCTG AAGCCATCCA GGACGTCATC 
CGCCACATCC GCGTCATCAA CGGCCGGGCC GTGGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004126.1
CDS	50..1231
Translation	MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVINGRAVEA

Target ORF information:

RefSeq Version	NM_004135.4
Organism	Homo sapiens(human)
Definition	Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma (IDH3G), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004135.4

ORF Insert Sequence:

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
ATGCACACTC CGGACATCGG GGGCCAGGGC ACAACATCTG AAGCCATCCA GGACGTCATC 
CGCCACATCC GCGTCATCAA CGGCCGGGCC GTGGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDH3G ( NM_004135.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_004126.1 Homo sapiens isocitrate dehydrogenase 3 (NAD(+)) gamma (IDH3G), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10112
Clone ID Related Accession (Same CDS sequence)	NM_004135.4 , NM_004135.3
Accession Version	NM_004135.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI831096.1, BC000933.2 and BC001902.1. On Jun 13, 2012 this sequence version replaced NM_004135.2. Transcript Variant: This variant (1) encodes the longer isoform (a). ##Evidence-Data-START## Transcript exon combination :: U40272.1, Z68907.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
ATGCACACTC CGGACATCGG GGGCCAGGGC ACAACATCTG AAGCCATCCA GGACGTCATC 
CGCCACATCC GCGTCATCAA CGGCCGGGCC GTGGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_004126.1
CDS	198..1379
Translation	MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENMHTPDIGGQGTTSEAIQDVIRHIRVINGRAVEA

Target ORF information:

RefSeq Version	NM_004135.3
Organism	Homo sapiens(human)
Definition	Homo sapiens isocitrate dehydrogenase 3 (NAD(+)) gamma (IDH3G), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_004135.3

ORF Insert Sequence:

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
ATGCACACTC CGGACATCGG GGGCCAGGGC ACAACATCTG AAGCCATCCA GGACGTCATC 
CGCCACATCC GCGTCATCAA CGGCCGGGCC GTGGAGGCCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDH3G ( NM_174869.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_777358.1 Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma (IDH3G), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10116
Clone ID Related Accession (Same CDS sequence)	NM_174869.3 , NM_174869.2
Accession Version	NM_174869.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI831096.1, BC000933.2 and U69268.1. On May 31, 2019 this sequence version replaced NM_174869.2. Transcript Variant: This variant (2) differs in the 3' UTR and coding region resulting in a frameshift, compared to variant 1. Variant 2 encodes a shorter isoform (b) with a distinct C-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.120379.1, SRR1163658.2337.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
GTGAGGTTCC CCTCGCACCC TACCCTGCTG CCCCGCCCAG TCTCCCCCTG CAGCCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777358.1
CDS	50..1192
Translation	MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVRFPSHPTLLPRPVSPCSLL

Target ORF information:

RefSeq Version	NM_174869.3
Organism	Homo sapiens(human)
Definition	Homo sapiens isocitrate dehydrogenase (NAD(+)) 3 non-catalytic subunit gamma (IDH3G), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174869.3

ORF Insert Sequence:

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
GTGAGGTTCC CCTCGCACCC TACCCTGCTG CCCCGCCCAG TCTCCCCCTG CAGCCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IDH3G ( NM_174869.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_777358.1 Homo sapiens isocitrate dehydrogenase 3 (NAD(+)) gamma (IDH3G), transcript variant 2, mRNA.

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CloneID	OHu10116
Clone ID Related Accession (Same CDS sequence)	NM_174869.3 , NM_174869.2
Accession Version	NM_174869.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1143bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-14
Organism	Homo sapiens(human)
Product	isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI831096.1, BC000933.2, U69268.1 and BC001902.1. On Jun 15, 2012 this sequence version replaced NM_174869.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding region resulting in a frameshift, compared to variant 1. Variant 2 encodes a shorter isoform (b) with a distinct C-terminus, compared to isoform a. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.2337.1, SRR1163655.91130.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
GTGAGGTTCC CCTCGCACCC TACCCTGCTG CCCCGCCCAG TCTCCCCCTG CAGCCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_777358.1
CDS	198..1340
Translation	MALKVATVAGSAAKAVLGPALLCRPWEVLGAHEVPSRNIFSEQTIPPSAKYGGRHTVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSSNADEEDIRNAIMAIRRNRVALKGNIETNHNLPPSHKSRNNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVRENTEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGDGLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVGGPGLVAGANYGHVYAVFETATRNTGKSIANKNIANPTATLLASCMMLDHLKLHSYATSIRKAVLASMDNENVRFPSHPTLLPRPVSPCSLL

Target ORF information:

RefSeq Version	NM_174869.2
Organism	Homo sapiens(human)
Definition	Homo sapiens isocitrate dehydrogenase 3 (NAD(+)) gamma (IDH3G), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_174869.2

ORF Insert Sequence:

ATGGCGCTGA AGGTAGCGAC CGTCGCCGGC AGCGCCGCGA AGGCGGTGCT CGGGCCAGCC 
CTTCTCTGCC GTCCCTGGGA GGTTCTAGGC GCCCACGAGG TCCCCTCGAG GAACATCTTT 
TCAGAACAAA CAATTCCTCC GTCCGCTAAG TATGGCGGGC GGCACACGGT GACCATGATC 
CCAGGGGATG GCATCGGGCC AGAGCTCATG CTGCATGTCA AGTCCGTCTT CAGGCACGCA 
TGTGTACCAG TGGACTTTGA AGAGGTGCAC GTGAGTTCCA ATGCTGATGA AGAGGACATT 
CGCAATGCCA TCATGGCCAT CCGCCGGAAC CGCGTGGCCC TGAAGGGCAA CATCGAAACC 
AACCATAACC TGCCACCGTC GCACAAATCT CGAAACAACA TCCTTCGCAC CAGCCTGGAC 
CTCTATGCCA ACGTCATCCA CTGTAAGAGC CTTCCAGGCG TGGTGACCCG GCACAAGGAC 
ATAGACATCC TCATTGTCCG GGAGAACACA GAGGGCGAGT ACAGCAGCCT GGAGCATGAG 
AGTGTGGCGG GAGTGGTGGA GAGCCTGAAG ATCATCACCA AGGCCAAGTC CCTGCGCATT 
GCCGAGTATG CCTTCAAGCT GGCGCAGGAG AGCGGGCGCA AGAAAGTGAC GGCCGTGCAC 
AAGGCCAACA TCATGAAACT GGGCGATGGG CTTTTCCTCC AGTGCTGCAG GGAGGTGGCA 
GCCCGCTACC CTCAGATCAC CTTCGAGAAC ATGATTGTGG ATAACACCAC CATGCAGCTG 
GTGTCCCGGC CCCAGCAGTT TGATGTCATG GTGATGCCCA ATCTCTATGG CAACATCGTC 
AACAATGTCT GCGCGGGACT GGTCGGGGGC CCAGGCCTTG TGGCTGGGGC CAACTATGGC 
CATGTGTACG CGGTGTTTGA AACAGCTACG AGGAACACCG GCAAGAGTAT CGCCAATAAG 
AACATCGCCA ACCCCACGGC CACCCTGCTG GCCAGCTGCA TGATGCTGGA CCACCTCAAG 
CTGCACTCCT ATGCCACCTC CATCCGTAAG GCTGTCCTGG CATCCATGGA CAATGAGAAT 
GTGAGGTTCC CCTCGCACCC TACCCTGCTG CCCCGCCCAG TCTCCCCCTG CAGCCTCCTG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Molecular mechanism of the allosteric regulation of the ?? heterodimer of human NAD-dependent isocitrate dehydrogenase.
Scientific reports740921(2017 Jan)
Ma T,Peng Y,Huang W,Ding J

Role of alpha-Asp181, beta-Asp192, and gamma-Asp190 in the distinctive subunits of human NAD-specific isocitrate dehydrogenase.
Biochemistry46(18)5391-7(2007 May)
Bzymek KP,Colman RF

Identification of Mn2+-binding aspartates from alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase.
The Journal of biological chemistry281(30)21073-81(2006 Jul)
Soundar S,O'hagan M,Fomulu KS,Colman RF

Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase.
The Journal of biological chemistry278(52)52146-53(2003 Dec)
Soundar S,Park JH,Huh TL,Colman RF

Systematic subcellular localization of novel proteins identified by large-scale cDNA sequencing.
EMBO reports1(3)287-92(2000 Sep)
Simpson JC,Wellenreuther R,Poustka A,Pepperkok R,Wiemann S

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IDH3G cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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