FREM2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	FREM2
Entrez Gene ID	341640
Full Name	FRAS1 related extracellular matrix protein 2
Synonyms	FRASRS2
General protein information	Preferred Names FRAS1 related extracellular matrix protein 2 Names FRAS1-related extracellular matrix protein 2 ECM3 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q13.3
Summary	This gene encodes an integral membrane protein containing numerous CSPG (chondroitin sulfate proteoglycan element) repeats and Calx-beta domains. The encoded protein localizes to the basement membrane, forming a ternary complex that plays a role in epidermal-dermal interactions. This protein is important for the integrity of skin and renal epithelia. Mutations in this gene are associated with Fraser syndrome. [provided by RefSeq, Apr 2014].
Disorder MIM:	608945
Disorder Html:	Fraser syndrome 2, 617666 (3)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017020554.1	XP_016876043.1	FRAS1-related extracellular matrix protein 2 isoform X1
NM_207361.6	NP_997244.4	FRAS1-related extracellular matrix protein 2 precursor
NM_207361.5	NP_997244.4	FRAS1-related extracellular matrix protein 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	FREM2	NP_997244.3
Danio rerio (zebrafish)	frem2b	NP_001131133.1
Pan troglodytes (chimpanzee)	FREM2	XP_522664.2
	FREM2	XP_001086486.1
Bos taurus (cattle)	FREM2	XP_002691845.1
Canis lupus familiaris (dog)	FREM2	XP_005635476.1
Rattus norvegicus (Norway rat)	Frem2	NP_001232907.1
Mus musculus (house mouse)	Frem2	NP_766450.2
Gallus gallus (chicken)	FREM2	XP_417087.3
Danio rerio (zebrafish)	frem2a	NP_001131129.1
Xenopus tropicalis (tropical clawed frog)	LOC100496696	XP_004912033.1

Gene Ontology
Bibliography

Related articles in PubMed

Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.
Kohl S, Hwang DY, Dworschak GC, Hilger AC, Saisawat P, Vivante A, Stajic N, Bogdanovic R, Reutter HM, Kehinde EO, Tasic V, Hildebrandt F
Journal of the American Society of Nephrology : JASN25(9)1917-22(2014 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In 15 of 590 families, we identified recessive mutations in the genes FRAS1, FREM2, GRIP1, FREM1, ITGA8, and GREM1, all of which function in the interaction of the ureteric bud and the metanephric mesenchyme.
Title: Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.

Amplification of the FREM2 gene is associated with mesenchymal differentiation in gliosarcoma.
Title: Amplification of the STOML3, FREM2, and LHFP genes is associated with mesenchymal differentiation in gliosarcoma.

Heterozygous missense mutations in FREM2 cause non-syndromic congenital abnormalities of the kidney and urinary tract in humans.
Title: Identification of two novel CAKUT-causing genes by massively parallel exon resequencing of candidate genes in patients with unilateral renal agenesis.

1 new mutation in FREM2 was identified in families with Fraser syndrome.
Title: Molecular study of 33 families with Fraser syndrome new data and mutation review.

report on 2 fetuses affected by Fraser syndrome (FS); a homozygous IVS14 + 1G -- A mutation in FREM2 was indentified; present report provides additional evidence that FS may be caused by complete or near-complete lack or loss of function of FREM2 protein
Title: Fraser syndrome due to homozygosity for a splice site mutation of FREM2.

The following FREM2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FREM2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu84526	XM_017020554.1 Latest version!	Homo sapiens FRAS1 related extracellular matrix 2 (FREM2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu28448	NM_207361.6 Latest version!	Homo sapiens FRAS1 related extracellular matrix 2 (FREM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu28448	NM_207361.5	Homo sapiens FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FREM2 ( XM_017020554.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016876043.1 Homo sapiens FRAS1 related extracellular matrix 2 (FREM2), transcript variant X2, mRNA.

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CloneID	OHu84526
Clone ID Related Accession (Same CDS sequence)	XM_017020554.1
Accession Version	XM_017020554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000013.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACTCAG CCGGGACTCC CGGGTTATCC TCGCGCCGGA CAGGCAACTC CACCAGCTTT 
CAACCAGGAC CGCCACCGCC GCCCCGGCTG CTGCTGCTGC TGCTGCTTCT CCTGTCACTG 
GTAAGCCGCG TCCCGGCACA GCCCGCTGCC TTCGGCAGGG CGTTGCTGTC CCCTGGTCTC 
GCGGGGGCTG CAGGGGTCCC TGCTGAGGAG GCCATAGTGC TGGCGAACCG CGGACTCCGG 
GTGCCTTTCG GCCGTGAAGT CTGGCTGGAT CCCCTGCATG ACCTGGTGTT GCAGGTGCAG 
CCCGGGGACC GCTGCGCGGT TTCGGTACTA GACAACGACG CACTGGCCCA GCGACCGGGC 
CGCCTGAGTC CCAAGCGCTT CCCGTGCGAC TTTGGCCCTG GCGAGGTGCG CTACTCTCAC 
CTGGGCGCGC GCAGCCCGTC TCGGGACCGC GTCCGGCTGC AGCTGCGCTA TGACGCGCCC 
GGAGGGGCAG TAGTGCTACC ACTGGTACTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACTC GGAACTTGCC TCTGGTCGTG GAAGAGCTGC TGGGGACCAG CAATGCCCTG 
GACGCGCGGA GCCTGGAGTT CGCCTTCCAG CCCGAGACAG AGGAGTGCCG CGTGGGCATC 
CTGTCCGGCT TGGGCGCGCT GCCTCGCTAT GGAGAACTCC TCCACTACCC GCAGGTCCCT 
GGAGGAGCCA GAGAGGGAGG CGCCCCGGAG ACTCTCCTGA TGGACTGCAA AGCTTTCCAG 
GAACTAGGCG TGCGCTATCG CCACACAGCC GCCAGTCGCT CACCAAACAG GGACTGGATA 
CCCATGGTGG TGGAGCTGCG TTCACGAGGG GCTCCTGTGG GCAGCCCTGC TTTGAAACGC 
GAGCACTTCC AGGTTCTGGT GAGGATCCGA GGAGGGGCCG AGAACACTGC ACCCAAGCCC 
AGTTTCGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TACTGACGGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGTCT CCCTCTGACC TGTTGATCTT CAACCTTACT 
TCTCCATTCC AGCCTGGCCA GGGCTACTTG GTGAGCACCG ATGATCGCAG CCTGCCCCTT 
TCCTCCTTCA CTCAGAGGGA TCTGCGGCTC CTGAAGATTG CCTACCAGCC CCCTTCTGAA 
GACTCTGACC AGGAGCGCCT CTTTGAACTG GAATTGGAGG TAGTGGATCT AGAAGGAGCA 
GCTTCAGACC CTTTTGCCTT CATGGTAGTG GTGAAGCCCA TGAACACAAT GGCTCCGGTG 
GTCACCCGGA ATACCGGTCT TATTCTCTAT GAGGGTCAGT CTCGGCCCCT CACAGGCCCT 
GCAGGCAGTG GTCCGCAAAA CTTGGTCATC AGCGATGAGG ATGACCTAGA AGCAGTGCGG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CACCTTGTCA TTCTGGGTGC TTCCAGTGGC 
AGCTCTGCTC CCAAGAGCTT TACAGTGGCT GAGCTGGCAG CCGGCCAGGT GGTCTACCAG 
CATGATGACA GAGACGGCTC GCTGAGCGAC AACCTGGTGC TTCGCATGGT GGATGGAGGA 
GGCAGGCACC AGGTACAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCTGTTCTCA ATGCCAACAC GGGGCTGACA CTGGCAGAGG GTGAAACAGT GCCCATCCTG 
CCCCTTTCCC TGAGTGCAAC TGACATGGAT TCAGATGATT CTCTGCTGCT TTTTGTGCTG 
GAGTCACCCT TCTTAACTAC GGGGCATCTG CTTCTCCGCC AAACTCACCC TCCCCATGAG 
AAGCAGGAAC TTCTCAGAGG CCTTTGGAGG AAGGAGGGGG CATTTTATGA GCGAACAGTG 
ACAGAGTGGC AGCAGCAGGA CATAACAGAG GGCAGGCTGT TCTATAGACA CTCTGGGCCC 
CATAGTCCTG GGCCAGTCAC AGACCAGTTC ACATTTAGAG TCCAGGATAA CCATGACCCT 
CCTAATCAGT CCGGGCTACA GCGGTTTGTG ATTCGTATCC ATCCTGTGGA TCGCCTCCCT 
CCGGAGCTGG GCAGTGGCTG TCCCCTTCGT ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTGCG CTACACTGAC CTGGACACAG ATGACCGAGA ACTACGTTAC 
ACAGTGACTC AGCCCCCCAC AGACACAGAC GAAAATCACC TGCCAGCCCC ACTGGGTACC 
TTGGTCTTGA CTGACAACCC CTCAGTCGTG GTGACCCATT TTACCCAAGC CCAGATCAAC 
CATCATAAAA TTGCTTACAG ACCCCCGGGT CAAGAACTGG GCGTGGCTAC TCGAGTGGCC 
CAGTTCCAGT TCCAGGTGGA AGACCGAGCT GGGAATGTGG CTCCAGGTAC CTTTACCCTT 
TACTTGCATC CCGTGGACAA CCAGCCACCT GAGATCCTCA ACACCGGCTT CACTATTCAG 
GAGAAGGGTC ACCACATCCT GAGTGAGACA GAGTTGCACG TGAATGATGT AGACACTGAT 
GTTGCCCATA TCTCTTTCAC TCTCACTCAG GCACCCAAAC ATGGCCACAT GAGAGTGTCT 
GGACAGATCC TGCATGTAGG GGGTCTCTTC CACTTGGAGG ACATAAAACA GGGCCGAGTT 
TCCTATGCCC ATAATGGGGA CAAGTCCCTG ACTGATAGCT GCTCCTTGGA AGTCAGTGAC 
AGACATCATG TGGTGCCCAT CACTCTCAGA GTAAATGTCC GGCCAGTGGA TGATGAAGTG 
CCCATACTGA GCCATCCTAC TGGCACTCTG GAGTCCTATC TAGATGTCTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATTAAG GGGACCAATG AGGAAACTGA TGACTTGATG 
TTGACTTTCC TCTTGGAAGA TCCACCTTTG TATGGGGAAA TCTTGGTCAA TGGCATTCCA 
GCAGAGCAGT TTACTCAAAG GGACATCTTG GAGGGCTCTG TTGTATATAC CCACACCAGT 
GGTGAGATAG GCCTATTGCC TAAAGCGGAT TCTTTTAACC TGAGTCTGTC AGATATGTCT 
CAAGAATGGA GAATTGGTGG CAATACTATC CAAGGAGTTA CTATATGGGT GACCATCCTG 
CCTGTTGATA GCCAGGCCCC AGAAATCTTT GTAGGTGAAC AGTTGATAGT AATGGAAGGT 
GATAAAAGTG TTATAACATC AGTGCATATA AGTGCTGAAG ATGTCGACTC CCTGAATGAT 
GACATCTTGT GCACTATAGT TATTCAGCCT ACTTCAGGTT ATGTTGAAAA CATTTCTCCA 
GCACCAGGCT CTGAGAAATC AAGAGCAGGG ATTGCCATAA GTGCTTTCAA CTTGAAAGAT 
CTCAGGCAGG GCCACATAAA CTATGTCCAG AGTGTCCATA AAGGGGTGGA ACCTGTGGAG 
GACCGATTTG TATTTCGTTG TTCTGATGGC ATTAACTTTT CAGAGAGACA GTTCTTCCCC 
ATTGTAATCA TTCCCACCAA TGATGAACAG CCAGAGATGT TTATGAGAGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT AATTGATACA CCCATTCTCA ATGCTGCTGA TGCTGATGTT 
CCCCTGGATG ATTTAACTTT CACTATTACC CAATTCCCCA CTCATGGTCA CATCATGAAT 
CAGCTGATAA ATGGCACGGT TTTGGTCGAA AGCTTCACCT TGGATCAGAT CATAGAGAGT 
TCCAGCATTA TTTATGAGCA TGATGACTCC GAGACCCAGG AAGACAGTTT TGTGATTAAA 
CTAACAGATG GGAAGCACTC TGTGGAAAAG ACGGTCCTCA TTATAGTTAT CCCTGTTGAT 
GATGAGACGC CCAGAATGAC TATCAATAAT GGACTAGAAA TAGAAATTGG GGATACCAAG 
ATTATCAACA ACAAAATATT AATGGCAACA GATTTAGATT CAGAAGACAA ATCTTTGGTT 
TATATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACGAAAACC TACTGGTGCC 
TTTGAAAATA TCACACTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAACTTAATT 
CAGTATGTCC ATTTGGGGCA AGAGGGCATT CGGGACCTAA TTAAATTTGA TGTGACTGAT 
GGAATAAATC CCCTCATAGA TCGTTACTTT TATGTGTCCA TCGGGAGCAT TGACATTGTC 
TTCCCTGATG TGATAAGTAA GGGAGTGTCC TTGAAAGAAG GTGGCAAAGT CACTCTTACA 
ACAGACCTAC TAAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTGGT TTTTACCATC 
ACCAGGGCTC CCATGCGAGG TCACCTGGAA TGCACGGATC AGCCTGGTGT GTCCATCACG 
TCTTTCACTC AGCTGCAACT GGCTGGAAAC AAAATCTACT ACATCCACAC AGCTGATGAT 
GAAGTGAAAA TGGACAGTTT TGAGTTTCAA GTCACCGATG GACGTAACCC TGTCTTTCGG 
ACATTCCGTA TCTCCATTAG CGATGTGGAC AATAAAAAGC CAGTGGTCAC CATCCACAAG 
CTGGTTGTCA GTGAAAGTGA AAACAAGCTG ATTACTCCTT TTGAGCTCAC TGTCGAAGAC 
AGAGATACTC CTGACAAGCT CCTGAAATTC ACTATCACCC AGGTGCCTAT TCATGGCCAT 
CTCCTATTCA ACAATACCAG ACCTGTCATG GTTTTTACCA AGCAAGACTT GAATGAAAAC 
TTAATCAGCT ACAAACATGA TGGCACTGAG TCAAGTGAAG ATAGCTTCTC CTTCACAGTG 
ACTGATGGCA CCCATACAGA CTTCTATGTT TTTCCTGATA CGGTGTTTGA AACAAGGAGA 
CCCCAAGTGA TGAAGATCCA GGTCTTGGCT GTTGACAACA GTGTCCCCCA AATCGCAGTG 
AATAAGGGGG CCTCTACACT TCGCACTCTA GCCACTGGCC ACTTGGGGTT CATGATCACA 
AGCAAAATAT TGAAAGTGGA GGACAGAGAC AGCTTACACA TTTCTCTTAG ATTTATCGTG 
ACAGAGGCCC CTCAACATGG ATATCTTCTC AACCTGGACA AAGGCAACCA CAGCATCACT 
CAGTTCACAC AAGCTGACAT TGATGACATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCACAA GTGATATGTT CTATTTTGCA GTTGAAGATG GTGCAAAAAT CCCACAGAAG 
GAGCGACAAG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016876043.1
CDS	305..5599
Translation	MHSAGTPGLSSRRTGNSTSFQPGPPPPPRLLLLLLLLLSLVSRVPAQPAAFGRALLSPGLAGAAGVPAEEAIVLANRGLRVPFGREVWLDPLHDLVLQVQPGDRCAVSVLDNDALAQRPGRLSPKRFPCDFGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAVVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAFQPETEECRVGILSGLGALPRYGELLHYPQVPGGAREGGAPETLLMDCKAFQELGVRYRHTAASRSPNRDWIPMVVELRSRGAPVGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESPSDLLIFNLTSPFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEVVDLEGAASDPFAFMVVVKPMNTMAPVVTRNTGLILYEGQSRPLTGPAGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGASSGSSAPKSFTVAELAAGQVVYQHDDRDGSLSDNLVLRMVDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPLSLSATDMDSDDSLLLFVLESPFLTTGHLLLRQTHPPHEKQELLRGLWRKEGAFYERTVTEWQQQDITEGRLFYRHSGPHSPGPVTDQFTFRVQDNHDPPNQSGLQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPTDTDENHLPAPLGTLVLTDNPSVVVTHFTQAQINHHKIAYRPPGQELGVATRVAQFQFQVEDRAGNVAPGTFTLYLHPVDNQPPEILNTGFTIQEKGHHILSETELHVNDVDTDVAHISFTLTQAPKHGHMRVSGQILHVGGLFHLEDIKQGRVSYAHNGDKSLTDSCSLEVSDRHHVVPITLRVNVRPVDDEVPILSHPTGTLESYLDVLENGATEITANVIKGTNEETDDLMLTFLLEDPPLYGEILVNGIPAEQFTQRDILEGSVVYTHTSGEIGLLPKADSFNLSLSDMSQEWRIGGNTIQGVTIWVTILPVDSQAPEIFVGEQLIVMEGDKSVITSVHISAEDVDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFNLKDLRQGHINYVQSVHKGVEPVEDRFVFRCSDGINFSERQFFPIVIIPTNDEQPEMFMREFMVMEGMSLVIDTPILNAADADVPLDDLTFTITQFPTHGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKTVLIIVIPVDDETPRMTINNGLEIEIGDTKIINNKILMATDLDSEDKSLVYIIRYGPGHGLLQRRKPTGAFENITLGMNFTQDEVDRNLIQYVHLGQEGIRDLIKFDVTDGINPLIDRYFYVSIGSIDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGVSITSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHKLVVSESENKLITPFELTVEDRDTPDKLLKFTITQVPIHGHLLFNNTRPVMVFTKQDLNENLISYKHDGTESSEDSFSFTVTDGTHTDFYVFPDTVFETRRPQVMKIQVLAVDNSVPQIAVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHISLRFIVTEAPQHGYLLNLDKGNHSITQFTQADIDDMKICYVLREGANATSDMFYFAVEDGAKIPQKERQD

Target ORF information:

RefSeq Version	XM_017020554.1
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 2 (FREM2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017020554.1

ORF Insert Sequence:

ATGCACTCAG CCGGGACTCC CGGGTTATCC TCGCGCCGGA CAGGCAACTC CACCAGCTTT 
CAACCAGGAC CGCCACCGCC GCCCCGGCTG CTGCTGCTGC TGCTGCTTCT CCTGTCACTG 
GTAAGCCGCG TCCCGGCACA GCCCGCTGCC TTCGGCAGGG CGTTGCTGTC CCCTGGTCTC 
GCGGGGGCTG CAGGGGTCCC TGCTGAGGAG GCCATAGTGC TGGCGAACCG CGGACTCCGG 
GTGCCTTTCG GCCGTGAAGT CTGGCTGGAT CCCCTGCATG ACCTGGTGTT GCAGGTGCAG 
CCCGGGGACC GCTGCGCGGT TTCGGTACTA GACAACGACG CACTGGCCCA GCGACCGGGC 
CGCCTGAGTC CCAAGCGCTT CCCGTGCGAC TTTGGCCCTG GCGAGGTGCG CTACTCTCAC 
CTGGGCGCGC GCAGCCCGTC TCGGGACCGC GTCCGGCTGC AGCTGCGCTA TGACGCGCCC 
GGAGGGGCAG TAGTGCTACC ACTGGTACTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACTC GGAACTTGCC TCTGGTCGTG GAAGAGCTGC TGGGGACCAG CAATGCCCTG 
GACGCGCGGA GCCTGGAGTT CGCCTTCCAG CCCGAGACAG AGGAGTGCCG CGTGGGCATC 
CTGTCCGGCT TGGGCGCGCT GCCTCGCTAT GGAGAACTCC TCCACTACCC GCAGGTCCCT 
GGAGGAGCCA GAGAGGGAGG CGCCCCGGAG ACTCTCCTGA TGGACTGCAA AGCTTTCCAG 
GAACTAGGCG TGCGCTATCG CCACACAGCC GCCAGTCGCT CACCAAACAG GGACTGGATA 
CCCATGGTGG TGGAGCTGCG TTCACGAGGG GCTCCTGTGG GCAGCCCTGC TTTGAAACGC 
GAGCACTTCC AGGTTCTGGT GAGGATCCGA GGAGGGGCCG AGAACACTGC ACCCAAGCCC 
AGTTTCGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TACTGACGGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGTCT CCCTCTGACC TGTTGATCTT CAACCTTACT 
TCTCCATTCC AGCCTGGCCA GGGCTACTTG GTGAGCACCG ATGATCGCAG CCTGCCCCTT 
TCCTCCTTCA CTCAGAGGGA TCTGCGGCTC CTGAAGATTG CCTACCAGCC CCCTTCTGAA 
GACTCTGACC AGGAGCGCCT CTTTGAACTG GAATTGGAGG TAGTGGATCT AGAAGGAGCA 
GCTTCAGACC CTTTTGCCTT CATGGTAGTG GTGAAGCCCA TGAACACAAT GGCTCCGGTG 
GTCACCCGGA ATACCGGTCT TATTCTCTAT GAGGGTCAGT CTCGGCCCCT CACAGGCCCT 
GCAGGCAGTG GTCCGCAAAA CTTGGTCATC AGCGATGAGG ATGACCTAGA AGCAGTGCGG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CACCTTGTCA TTCTGGGTGC TTCCAGTGGC 
AGCTCTGCTC CCAAGAGCTT TACAGTGGCT GAGCTGGCAG CCGGCCAGGT GGTCTACCAG 
CATGATGACA GAGACGGCTC GCTGAGCGAC AACCTGGTGC TTCGCATGGT GGATGGAGGA 
GGCAGGCACC AGGTACAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCTGTTCTCA ATGCCAACAC GGGGCTGACA CTGGCAGAGG GTGAAACAGT GCCCATCCTG 
CCCCTTTCCC TGAGTGCAAC TGACATGGAT TCAGATGATT CTCTGCTGCT TTTTGTGCTG 
GAGTCACCCT TCTTAACTAC GGGGCATCTG CTTCTCCGCC AAACTCACCC TCCCCATGAG 
AAGCAGGAAC TTCTCAGAGG CCTTTGGAGG AAGGAGGGGG CATTTTATGA GCGAACAGTG 
ACAGAGTGGC AGCAGCAGGA CATAACAGAG GGCAGGCTGT TCTATAGACA CTCTGGGCCC 
CATAGTCCTG GGCCAGTCAC AGACCAGTTC ACATTTAGAG TCCAGGATAA CCATGACCCT 
CCTAATCAGT CCGGGCTACA GCGGTTTGTG ATTCGTATCC ATCCTGTGGA TCGCCTCCCT 
CCGGAGCTGG GCAGTGGCTG TCCCCTTCGT ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTGCG CTACACTGAC CTGGACACAG ATGACCGAGA ACTACGTTAC 
ACAGTGACTC AGCCCCCCAC AGACACAGAC GAAAATCACC TGCCAGCCCC ACTGGGTACC 
TTGGTCTTGA CTGACAACCC CTCAGTCGTG GTGACCCATT TTACCCAAGC CCAGATCAAC 
CATCATAAAA TTGCTTACAG ACCCCCGGGT CAAGAACTGG GCGTGGCTAC TCGAGTGGCC 
CAGTTCCAGT TCCAGGTGGA AGACCGAGCT GGGAATGTGG CTCCAGGTAC CTTTACCCTT 
TACTTGCATC CCGTGGACAA CCAGCCACCT GAGATCCTCA ACACCGGCTT CACTATTCAG 
GAGAAGGGTC ACCACATCCT GAGTGAGACA GAGTTGCACG TGAATGATGT AGACACTGAT 
GTTGCCCATA TCTCTTTCAC TCTCACTCAG GCACCCAAAC ATGGCCACAT GAGAGTGTCT 
GGACAGATCC TGCATGTAGG GGGTCTCTTC CACTTGGAGG ACATAAAACA GGGCCGAGTT 
TCCTATGCCC ATAATGGGGA CAAGTCCCTG ACTGATAGCT GCTCCTTGGA AGTCAGTGAC 
AGACATCATG TGGTGCCCAT CACTCTCAGA GTAAATGTCC GGCCAGTGGA TGATGAAGTG 
CCCATACTGA GCCATCCTAC TGGCACTCTG GAGTCCTATC TAGATGTCTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATTAAG GGGACCAATG AGGAAACTGA TGACTTGATG 
TTGACTTTCC TCTTGGAAGA TCCACCTTTG TATGGGGAAA TCTTGGTCAA TGGCATTCCA 
GCAGAGCAGT TTACTCAAAG GGACATCTTG GAGGGCTCTG TTGTATATAC CCACACCAGT 
GGTGAGATAG GCCTATTGCC TAAAGCGGAT TCTTTTAACC TGAGTCTGTC AGATATGTCT 
CAAGAATGGA GAATTGGTGG CAATACTATC CAAGGAGTTA CTATATGGGT GACCATCCTG 
CCTGTTGATA GCCAGGCCCC AGAAATCTTT GTAGGTGAAC AGTTGATAGT AATGGAAGGT 
GATAAAAGTG TTATAACATC AGTGCATATA AGTGCTGAAG ATGTCGACTC CCTGAATGAT 
GACATCTTGT GCACTATAGT TATTCAGCCT ACTTCAGGTT ATGTTGAAAA CATTTCTCCA 
GCACCAGGCT CTGAGAAATC AAGAGCAGGG ATTGCCATAA GTGCTTTCAA CTTGAAAGAT 
CTCAGGCAGG GCCACATAAA CTATGTCCAG AGTGTCCATA AAGGGGTGGA ACCTGTGGAG 
GACCGATTTG TATTTCGTTG TTCTGATGGC ATTAACTTTT CAGAGAGACA GTTCTTCCCC 
ATTGTAATCA TTCCCACCAA TGATGAACAG CCAGAGATGT TTATGAGAGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT AATTGATACA CCCATTCTCA ATGCTGCTGA TGCTGATGTT 
CCCCTGGATG ATTTAACTTT CACTATTACC CAATTCCCCA CTCATGGTCA CATCATGAAT 
CAGCTGATAA ATGGCACGGT TTTGGTCGAA AGCTTCACCT TGGATCAGAT CATAGAGAGT 
TCCAGCATTA TTTATGAGCA TGATGACTCC GAGACCCAGG AAGACAGTTT TGTGATTAAA 
CTAACAGATG GGAAGCACTC TGTGGAAAAG ACGGTCCTCA TTATAGTTAT CCCTGTTGAT 
GATGAGACGC CCAGAATGAC TATCAATAAT GGACTAGAAA TAGAAATTGG GGATACCAAG 
ATTATCAACA ACAAAATATT AATGGCAACA GATTTAGATT CAGAAGACAA ATCTTTGGTT 
TATATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACGAAAACC TACTGGTGCC 
TTTGAAAATA TCACACTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAACTTAATT 
CAGTATGTCC ATTTGGGGCA AGAGGGCATT CGGGACCTAA TTAAATTTGA TGTGACTGAT 
GGAATAAATC CCCTCATAGA TCGTTACTTT TATGTGTCCA TCGGGAGCAT TGACATTGTC 
TTCCCTGATG TGATAAGTAA GGGAGTGTCC TTGAAAGAAG GTGGCAAAGT CACTCTTACA 
ACAGACCTAC TAAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTGGT TTTTACCATC 
ACCAGGGCTC CCATGCGAGG TCACCTGGAA TGCACGGATC AGCCTGGTGT GTCCATCACG 
TCTTTCACTC AGCTGCAACT GGCTGGAAAC AAAATCTACT ACATCCACAC AGCTGATGAT 
GAAGTGAAAA TGGACAGTTT TGAGTTTCAA GTCACCGATG GACGTAACCC TGTCTTTCGG 
ACATTCCGTA TCTCCATTAG CGATGTGGAC AATAAAAAGC CAGTGGTCAC CATCCACAAG 
CTGGTTGTCA GTGAAAGTGA AAACAAGCTG ATTACTCCTT TTGAGCTCAC TGTCGAAGAC 
AGAGATACTC CTGACAAGCT CCTGAAATTC ACTATCACCC AGGTGCCTAT TCATGGCCAT 
CTCCTATTCA ACAATACCAG ACCTGTCATG GTTTTTACCA AGCAAGACTT GAATGAAAAC 
TTAATCAGCT ACAAACATGA TGGCACTGAG TCAAGTGAAG ATAGCTTCTC CTTCACAGTG 
ACTGATGGCA CCCATACAGA CTTCTATGTT TTTCCTGATA CGGTGTTTGA AACAAGGAGA 
CCCCAAGTGA TGAAGATCCA GGTCTTGGCT GTTGACAACA GTGTCCCCCA AATCGCAGTG 
AATAAGGGGG CCTCTACACT TCGCACTCTA GCCACTGGCC ACTTGGGGTT CATGATCACA 
AGCAAAATAT TGAAAGTGGA GGACAGAGAC AGCTTACACA TTTCTCTTAG ATTTATCGTG 
ACAGAGGCCC CTCAACATGG ATATCTTCTC AACCTGGACA AAGGCAACCA CAGCATCACT 
CAGTTCACAC AAGCTGACAT TGATGACATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCACAA GTGATATGTT CTATTTTGCA GTTGAAGATG GTGCAAAAAT CCCACAGAAG 
GAGCGACAAG ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM2 ( NM_207361.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_997244.4 Homo sapiens FRAS1 related extracellular matrix 2 (FREM2), mRNA.

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CloneID	OHu28448
Clone ID Related Accession (Same CDS sequence)	NM_207361.6 , NM_207361.5
Accession Version	NM_207361.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN291155.1, BN000687.1, KF455770.1, AL354819.15 and BX640686.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_207361.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BN000687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000280481.9/ ENSP00000280481.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCACTCAG CCGGGACTCC CGGGTTATCC TCGCGCCGGA CAGGCAACTC CACCAGCTTT 
CAACCAGGAC CGCCACCGCC GCCCCGGCTG CTGCTGCTGC TGCTGCTTCT CCTGTCACTG 
GTAAGCCGCG TCCCGGCACA GCCCGCTGCC TTCGGCAGGG CGTTGCTGTC CCCTGGTCTC 
GCGGGGGCTG CAGGGGTCCC TGCTGAGGAG GCCATAGTGC TGGCGAACCG CGGACTCCGG 
GTGCCTTTCG GCCGTGAAGT CTGGCTGGAT CCCCTGCATG ACCTGGTGTT GCAGGTGCAG 
CCCGGGGACC GCTGCGCGGT TTCGGTACTA GACAACGACG CACTGGCCCA GCGACCGGGC 
CGCCTGAGTC CCAAGCGCTT CCCGTGCGAC TTTGGCCCTG GCGAGGTGCG CTACTCTCAC 
CTGGGCGCGC GCAGCCCGTC TCGGGACCGC GTCCGGCTGC AGCTGCGCTA TGACGCGCCC 
GGAGGGGCAG TAGTGCTACC ACTGGTACTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACTC GGAACTTGCC TCTGGTCGTG GAAGAGCTGC TGGGGACCAG CAATGCCCTG 
GACGCGCGGA GCCTGGAGTT CGCCTTCCAG CCCGAGACAG AGGAGTGCCG CGTGGGCATC 
CTGTCCGGCT TGGGCGCGCT GCCTCGCTAT GGAGAACTCC TCCACTACCC GCAGGTCCCT 
GGAGGAGCCA GAGAGGGAGG CGCCCCGGAG ACTCTCCTGA TGGACTGCAA AGCTTTCCAG 
GAACTAGGCG TGCGCTATCG CCACACAGCC GCCAGTCGCT CACCAAACAG GGACTGGATA 
CCCATGGTGG TGGAGCTGCG TTCACGAGGG GCTCCTGTGG GCAGCCCTGC TTTGAAACGC 
GAGCACTTCC AGGTTCTGGT GAGGATCCGA GGAGGGGCCG AGAACACTGC ACCCAAGCCC 
AGTTTCGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TACTGACGGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGTCT CCCTCTGACC TGTTGATCTT CAACCTTACT 
TCTCCATTCC AGCCTGGCCA GGGCTACTTG GTGAGCACCG ATGATCGCAG CCTGCCCCTT 
TCCTCCTTCA CTCAGAGGGA TCTGCGGCTC CTGAAGATTG CCTACCAGCC CCCTTCTGAA 
GACTCTGACC AGGAGCGCCT CTTTGAACTG GAATTGGAGG TAGTGGATCT AGAAGGAGCA 
GCTTCAGACC CTTTTGCCTT CATGGTAGTG GTGAAGCCCA TGAACACAAT GGCTCCGGTG 
GTCACCCGGA ATACCGGTCT TATTCTCTAT GAGGGTCAGT CTCGGCCCCT CACAGGCCCT 
GCAGGCAGTG GTCCGCAAAA CTTGGTCATC AGCGATGAGG ATGACCTAGA AGCAGTGCGG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CACCTTGTCA TTCTGGGTGC TTCCAGTGGC 
AGCTCTGCTC CCAAGAGCTT TACAGTGGCT GAGCTGGCAG CCGGCCAGGT GGTCTACCAG 
CATGATGACA GAGACGGCTC GCTGAGCGAC AACCTGGTGC TTCGCATGGT GGATGGAGGA 
GGCAGGCACC AGGTACAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCTGTTCTCA ATGCCAACAC GGGGCTGACA CTGGCAGAGG GTGAAACAGT GCCCATCCTG 
CCCCTTTCCC TGAGTGCAAC TGACATGGAT TCAGATGATT CTCTGCTGCT TTTTGTGCTG 
GAGTCACCCT TCTTAACTAC GGGGCATCTG CTTCTCCGCC AAACTCACCC TCCCCATGAG 
AAGCAGGAAC TTCTCAGAGG CCTTTGGAGG AAGGAGGGGG CATTTTATGA GCGAACAGTG 
ACAGAGTGGC AGCAGCAGGA CATAACAGAG GGCAGGCTGT TCTATAGACA CTCTGGGCCC 
CATAGTCCTG GGCCAGTCAC AGACCAGTTC ACATTTAGAG TCCAGGATAA CCATGACCCT 
CCTAATCAGT CCGGGCTACA GCGGTTTGTG ATTCGTATCC ATCCTGTGGA TCGCCTCCCT 
CCGGAGCTGG GCAGTGGCTG TCCCCTTCGT ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTGCG CTACACTGAC CTGGACACAG ATGACCGAGA ACTACGTTAC 
ACAGTGACTC AGCCCCCCAC AGACACAGAC GAAAATCACC TGCCAGCCCC ACTGGGTACC 
TTGGTCTTGA CTGACAACCC CTCAGTCGTG GTGACCCATT TTACCCAAGC CCAGATCAAC 
CATCATAAAA TTGCTTACAG ACCCCCGGGT CAAGAACTGG GCGTGGCTAC TCGAGTGGCC 
CAGTTCCAGT TCCAGGTGGA AGACCGAGCT GGGAATGTGG CTCCAGGTAC CTTTACCCTT 
TACTTGCATC CCGTGGACAA CCAGCCACCT GAGATCCTCA ACACCGGCTT CACTATTCAG 
GAGAAGGGTC ACCACATCCT GAGTGAGACA GAGTTGCACG TGAATGATGT AGACACTGAT 
GTTGCCCATA TCTCTTTCAC TCTCACTCAG GCACCCAAAC ATGGCCACAT GAGAGTGTCT 
GGACAGATCC TGCATGTAGG GGGTCTCTTC CACTTGGAGG ACATAAAACA GGGCCGAGTT 
TCCTATGCCC ATAATGGGGA CAAGTCCCTG ACTGATAGCT GCTCCTTGGA AGTCAGTGAC 
AGACATCATG TGGTGCCCAT CACTCTCAGA GTAAATGTCC GGCCAGTGGA TGATGAAGTG 
CCCATACTGA GCCATCCTAC TGGCACTCTG GAGTCCTATC TAGATGTCTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATTAAG GGGACCAATG AGGAAACTGA TGACTTGATG 
TTGACTTTCC TCTTGGAAGA TCCACCTTTG TATGGGGAAA TCTTGGTCAA TGGCATTCCA 
GCAGAGCAGT TTACTCAAAG GGACATCTTG GAGGGCTCTG TTGTATATAC CCACACCAGT 
GGTGAGATAG GCCTATTGCC TAAAGCGGAT TCTTTTAACC TGAGTCTGTC AGATATGTCT 
CAAGAATGGA GAATTGGTGG CAATACTATC CAAGGAGTTA CTATATGGGT GACCATCCTG 
CCTGTTGATA GCCAGGCCCC AGAAATCTTT GTAGGTGAAC AGTTGATAGT AATGGAAGGT 
GATAAAAGTG TTATAACATC AGTGCATATA AGTGCTGAAG ATGTCGACTC CCTGAATGAT 
GACATCTTGT GCACTATAGT TATTCAGCCT ACTTCAGGTT ATGTTGAAAA CATTTCTCCA 
GCACCAGGCT CTGAGAAATC AAGAGCAGGG ATTGCCATAA GTGCTTTCAA CTTGAAAGAT 
CTCAGGCAGG GCCACATAAA CTATGTCCAG AGTGTCCATA AAGGGGTGGA ACCTGTGGAG 
GACCGATTTG TATTTCGTTG TTCTGATGGC ATTAACTTTT CAGAGAGACA GTTCTTCCCC 
ATTGTAATCA TTCCCACCAA TGATGAACAG CCAGAGATGT TTATGAGAGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT AATTGATACA CCCATTCTCA ATGCTGCTGA TGCTGATGTT 
CCCCTGGATG ATTTAACTTT CACTATTACC CAATTCCCCA CTCATGGTCA CATCATGAAT 
CAGCTGATAA ATGGCACGGT TTTGGTCGAA AGCTTCACCT TGGATCAGAT CATAGAGAGT 
TCCAGCATTA TTTATGAGCA TGATGACTCC GAGACCCAGG AAGACAGTTT TGTGATTAAA 
CTAACAGATG GGAAGCACTC TGTGGAAAAG ACGGTCCTCA TTATAGTTAT CCCTGTTGAT 
GATGAGACGC CCAGAATGAC TATCAATAAT GGACTAGAAA TAGAAATTGG GGATACCAAG 
ATTATCAACA ACAAAATATT AATGGCAACA GATTTAGATT CAGAAGACAA ATCTTTGGTT 
TATATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACGAAAACC TACTGGTGCC 
TTTGAAAATA TCACACTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAACTTAATT 
CAGTATGTCC ATTTGGGGCA AGAGGGCATT CGGGACCTAA TTAAATTTGA TGTGACTGAT 
GGAATAAATC CCCTCATAGA TCGTTACTTT TATGTGTCCA TCGGGAGCAT TGACATTGTC 
TTCCCTGATG TGATAAGTAA GGGAGTGTCC TTGAAAGAAG GTGGCAAAGT CACTCTTACA 
ACAGACCTAC TAAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTGGT TTTTACCATC 
ACCAGGGCTC CCATGCGAGG TCACCTGGAA TGCACGGATC AGCCTGGTGT GTCCATCACG 
TCTTTCACTC AGCTGCAACT GGCTGGAAAC AAAATCTACT ACATCCACAC AGCTGATGAT 
GAAGTGAAAA TGGACAGTTT TGAGTTTCAA GTCACCGATG GACGTAACCC TGTCTTTCGG 
ACATTCCGTA TCTCCATTAG CGATGTGGAC AATAAAAAGC CAGTGGTCAC CATCCACAAG 
CTGGTTGTCA GTGAAAGTGA AAACAAGCTG ATTACTCCTT TTGAGCTCAC TGTCGAAGAC 
AGAGATACTC CTGACAAGCT CCTGAAATTC ACTATCACCC AGGTGCCTAT TCATGGCCAT 
CTCCTATTCA ACAATACCAG ACCTGTCATG GTTTTTACCA AGCAAGACTT GAATGAAAAC 
TTAATCAGCT ACAAACATGA TGGCACTGAG TCAAGTGAAG ATAGCTTCTC CTTCACAGTG 
ACTGATGGCA CCCATACAGA CTTCTATGTT TTTCCTGATA CGGTGTTTGA AACAAGGAGA 
CCCCAAGTGA TGAAGATCCA GGTCTTGGCT GTTGACAACA GTGTCCCCCA AATCGCAGTG 
AATAAGGGGG CCTCTACACT TCGCACTCTA GCCACTGGCC ACTTGGGGTT CATGATCACA 
AGCAAAATAT TGAAAGTGGA GGACAGAGAC AGCTTACACA TTTCTCTTAG ATTTATCGTG 
ACAGAGGCCC CTCAACATGG ATATCTTCTC AACCTGGACA AAGGCAACCA CAGCATCACT 
CAGTTCACAC AAGCTGACAT TGATGACATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCACAA GTGATATGTT CTATTTTGCA GTTGAAGATG GTGGTGGAAA CAAGTTAACG 
TACCAGAATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACCTGGTC 
AATGAGGACT CCAAATTTCT AGATGTTGTT CTTAAACGTA GAGGTTACTT GGGAGAAACT 
TCTTTTATAA GTATTGGCAC AAGAGACAGA ACTGCAGAAA AAGACAAAGA CTTCAAGGGC 
AAAGCACAGA AACAAGTGCA GTTCAACCCA GGCCAGACCA GGGCCACATG GCGAGTGCGG 
ATCCTGAGTG ATGGGGAGCA TGAGCAGTCT GAAACCTTTC AGGTGGTACT CTCAGAGCCC 
GTGCTGGCTG CCTTGGAATT CCCCACAGTC GCCACTGTTG AGATCGTTGA TCCAGGAGAT 
GAGCCAACTG TGTTTATTCC CCAGTCCAAA TACTCCGTTG AAGAAGATGT TGGTGAGCTG 
TTCATTCCCA TCAGGAGGAG CGGAGATGTG AGCCAGGAGT TGATGGTGGT CTGTTATACC 
CAACAAGGAA CAGCAACTGG AACTGTGCCG ACTTCCGTGT TGTCTTACTC TGATTACATA 
TCCAGGCCTG AGGACCACAC CAGTGTTGTC CGCTTTGACA AAGATGAACG GGAGAAACTG 
TGTCGGATAG TCATAATTGA TGACTCTTTG TACGAGGAGG AGGAAACCTT CCATGTCCTT 
CTGAGCATGC CCATGGGGGG AAGAATCGGA TCAGAGTTCC CAGGGGCTCA AGTTACAATC 
GTTCCTGACA AAGATGATGA ACCCATCTTT TACTTCGGTG ATGTGGAATA CTCTGTGGAT 
GAGAGTGCTG GCTATGTGGA AGTGCAGGTG TGGAGAACGG GCACTGACCT GTCCAAGTCT 
TCTAGTGTCA CAGTGAGGTC TCGGAAAACA GATCCTCCCT CTGCAGATGC TGGAACAGAC 
TATGTGGGCA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA GCCTGTTCGT 
GTTGTCATTC TGGATGACCT TGGACAACCA GCGCTGGAGG GAATTGAGAA ATTTGAACTG 
GTGCTTCGCA TGCCTATGAA CGCAGCCCTT GGCGAGCCCA GCAAAGCCAC AGTGTCCATA 
AATGACTCTG TCTCCGATTT GCCTAAGATG CAATTCAAAG AACGAATATA TACTGGCAGC 
GAAAGTGATG GGCAGATAGT TACAATGATC CATAGGACTG GGGATGTCCA GTACAGATCT 
TCAGTGAGAT GCTACACCCG GCAGGGGTCT GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCAAACACTG ATACCTCCAT CATCACATTC CTCCCTGGTG AGACAGAAAA GCCCTGCATT 
CTTGAGCTGA TGGACGATGT GCTCTATGAG GAGGTAGAGG AGCTCCGCCT GGTACTCGGC 
ACTCCACAAA GCAACTCTCC CTTTGGGGCT GCAGTTGGTG AACAAAATGA AACTCTCATA 
AGGATCCGAG ATGATGCTGA TAAGACTGTT ATTAAATTTG GAGAAACCAA ATTTAGTGTC 
ACTGAACCCA AAGAACCTGG AGAGTCGGTG GTTATAAGAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AGGTTTCCAT TGTGAGAGTC CACACCAAGG ATGGCTCGGC CACCTCTGGA 
GAAGACTACC ACCCTGTGTC AGAAGAAATT GAGTTTAAGG AAGGGGAAAC CCAGCACGTG 
GTTGAAATCG AAGTTACCTT TGACGGGGTG AGAGAGATGA GAGAGGCCTT CACTGTTCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ATGCAGTTGA CGAAAGCCAT TGTGTACATA 
GAAGAAATGA GCAGCATGGC AGATGTCACT TTTCCTTCTG TCCCTCAAAT TGTATCCCTG 
TTGATGTATG ACGACACTTC CAAAGCTAAG GAGAGTGCTG AACCCATGTC TGGCTATCCT 
GTCATCTGTA TCACAGCTTG CAACCCCAAA TATTCAGACT ACGATAAAAC AGGCTCTATC 
TGTGCAAGTG AGAACATCAA TGACACTTTG ACGCGGTACC GGTGGCTGAT TAGTGCACCT 
GCGGGCCCTG ACGGTGTGAC CAGCCCTATG AGAGAAGTGG ACTTCGACAC CTTTTTTACG 
TCATCCAAGA TGGTCACACT GGACTCCATA TACTTTCAGC CTGGCTCCCG GGTACAGTGC 
GCAGCTCGTG CTGTGAACAC CAATGGGGAT GAAGGCCTGG AGCTCATGAG CCCTATTGTA 
ACCATCAGCA GAGAAGAAGG TCTTTGTCAG CCCCGTGTAC CTGGGGTTGT TGGAGCAGAG 
CCGTTCTCAG CTAAATTGCG CTACACAGGC CCTGAGGATG CAGACTACAC AAACCTTATC 
AAGCTCACTG TCACAATGCC ACACATAGAT GGCATGCTCC CCGTGATCTC CACTAGAGAG 
CTTTCCAACT TTGAGCTCAC CCTCAGCCCT GATGGCACAA GAGTTGGAAA CCACAAGTGC 
TCCAACCTCC TGGATTATAC TGAAGTGAAG ACTCATTATG GTTTCTTGAC TGATGCTACC 
AAAAATCCAG AAATAATTGG AGAGACATAT CCTTACCAGT ACAGCTTGTC CATCAGAGGT 
TCCACTACCT TGCGCTTCTA CCGGAACCTG AACCTAGAGG CCTGTTTATG GGAGTTCGTT 
AGCTACTATG ACATGTCAGA ACTCCTTGCT GACTGTGGTG GCACCATTGG AACAGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTATGTG ACCCTTCGAG TCCCTCTGTA TGTTTCCTAC 
GTGTTCCATT CCCCCGTGGG GGTAGGAGGC TGGCAGCATT TTGACTTGAA GTCAGAGCTT 
CGTCTAACTT TTGTGTACGA CACCGCCATC TTGTGGAATG ATGGAATTGG CAGCCCCCCA 
GAGGCTGAAC TTCAAGGTTC TCTCTATCCA ACCAGCATGC GCATCGGTGA TGAGGGGCGC 
TTGGCCGTGC ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TATTTGTGCT GTCACATCCC 
GCATCCTTTA CAAGCTCAGT GATCATGTCA GCTGATCATC CAGGCCTGAC ATTTTCCCTC 
CGCCTCATAA GGAGTGAACC AACCTATAAC CAGCCAGTAC AGCAGTGGAG CTTTGTCTCT 
GACTTTGCCG TACGTGACTA CTCAGGGACC TATACTGTGA AGCTGGTGCC ATGCACTGCC 
CCATCACATC AGGAATACCG CCTGCCAGTC ACCTGCAACC CCAGAGAACC TGTCACCTTT 
GACCTTGACA TCCGATTCCA ACAGGTCAGT GATCCAGTGG CTGCTGAGTT TAGCTTGAAC 
ACCCAAATGT ACCTGCTCTC TAAGAAGAGT CTCTGGTTGT CTGATGGATC CATGGGATTC 
GGGCAAGAGA GTGATGTTGC TTTTGCAGAA GGTGATATAA TTTATGGTCG TGTCATGGTG 
GATCCTGTCC AGAATCTGGG TGACTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTATGC 
ACTGGAGCTG ATGGCTATGT TCCCAAGTAT AGTCCAATGA ATGCAGAATA TGGCTGCTTA 
GCCGACTCTC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA GCCAGAGACA 
CAAGCGACCA GTTTTGGAAA TGTCCTATTT AATGCCAAAC TAGCAGTGGA TGACCCTGAA 
GCCATTCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTCG CTCTAGGCCG AGAATGGTAT ATACATACGA TCTATACAGT GAGATCGAAA 
GACAATGCCA ATCGAGGTAT TGGCAAAAGA AGTGTGGAGT ACCATTCTCT GGTGAGTCAA 
GGAAAGCCCC AATCCACCAC CAAGAGCCGG AAGAAGAGAG AGATCAGGAG CACACCCTCA 
CTGGCATGGG AGATTGGTGC TGAAAACAGT CGAGGAACAA ACATCCAGCA CATTGCCCTG 
GACCGCACCA AGAGGCAGAT CCCCCATGGG AGAGCACCTC CAGATGGCAT CCTCCCCTGG 
GAGCTCAACA GCCCCAGCTC TGCAGTCAGC CTGGTCACTG TGGTGGGAGG CACCACGGTA 
GGGTTACTCA CCATCTGCCT CACTGTCATT GCAGTGCTGA TGTGCAGGGG CAAGGAAAGT 
TTCAGGGGGA AGGATGCCCC GAAAGGCTCC AGCAGCAGTG AGCCCATGGT GCCCCCACAG 
AGCCATCACA ATGACAGCTC AGAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997244.4
CDS	269..9778
Translation	MHSAGTPGLSSRRTGNSTSFQPGPPPPPRLLLLLLLLLSLVSRVPAQPAAFGRALLSPGLAGAAGVPAEEAIVLANRGLRVPFGREVWLDPLHDLVLQVQPGDRCAVSVLDNDALAQRPGRLSPKRFPCDFGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAVVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAFQPETEECRVGILSGLGALPRYGELLHYPQVPGGAREGGAPETLLMDCKAFQELGVRYRHTAASRSPNRDWIPMVVELRSRGAPVGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESPSDLLIFNLTSPFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEVVDLEGAASDPFAFMVVVKPMNTMAPVVTRNTGLILYEGQSRPLTGPAGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGASSGSSAPKSFTVAELAAGQVVYQHDDRDGSLSDNLVLRMVDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPLSLSATDMDSDDSLLLFVLESPFLTTGHLLLRQTHPPHEKQELLRGLWRKEGAFYERTVTEWQQQDITEGRLFYRHSGPHSPGPVTDQFTFRVQDNHDPPNQSGLQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPTDTDENHLPAPLGTLVLTDNPSVVVTHFTQAQINHHKIAYRPPGQELGVATRVAQFQFQVEDRAGNVAPGTFTLYLHPVDNQPPEILNTGFTIQEKGHHILSETELHVNDVDTDVAHISFTLTQAPKHGHMRVSGQILHVGGLFHLEDIKQGRVSYAHNGDKSLTDSCSLEVSDRHHVVPITLRVNVRPVDDEVPILSHPTGTLESYLDVLENGATEITANVIKGTNEETDDLMLTFLLEDPPLYGEILVNGIPAEQFTQRDILEGSVVYTHTSGEIGLLPKADSFNLSLSDMSQEWRIGGNTIQGVTIWVTILPVDSQAPEIFVGEQLIVMEGDKSVITSVHISAEDVDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFNLKDLRQGHINYVQSVHKGVEPVEDRFVFRCSDGINFSERQFFPIVIIPTNDEQPEMFMREFMVMEGMSLVIDTPILNAADADVPLDDLTFTITQFPTHGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKTVLIIVIPVDDETPRMTINNGLEIEIGDTKIINNKILMATDLDSEDKSLVYIIRYGPGHGLLQRRKPTGAFENITLGMNFTQDEVDRNLIQYVHLGQEGIRDLIKFDVTDGINPLIDRYFYVSIGSIDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGVSITSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHKLVVSESENKLITPFELTVEDRDTPDKLLKFTITQVPIHGHLLFNNTRPVMVFTKQDLNENLISYKHDGTESSEDSFSFTVTDGTHTDFYVFPDTVFETRRPQVMKIQVLAVDNSVPQIAVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHISLRFIVTEAPQHGYLLNLDKGNHSITQFTQADIDDMKICYVLREGANATSDMFYFAVEDGGGNKLTYQNFRLNWAWISFEKEYYLVNEDSKFLDVVLKRRGYLGETSFISIGTRDRTAEKDKDFKGKAQKQVQFNPGQTRATWRVRILSDGEHEQSETFQVVLSEPVLAALEFPTVATVEIVDPGDEPTVFIPQSKYSVEEDVGELFIPIRRSGDVSQELMVVCYTQQGTATGTVPTSVLSYSDYISRPEDHTSVVRFDKDEREKLCRIVIIDDSLYEEEETFHVLLSMPMGGRIGSEFPGAQVTIVPDKDDEPIFYFGDVEYSVDESAGYVEVQVWRTGTDLSKSSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQPVRVVILDDLGQPALEGIEKFELVLRMPMNAALGEPSKATVSINDSVSDLPKMQFKERIYTGSESDGQIVTMIHRTGDVQYRSSVRCYTRQGSAQVMMDFEERPNTDTSIITFLPGETEKPCILELMDDVLYEEVEELRLVLGTPQSNSPFGAAVGEQNETLIRIRDDADKTVIKFGETKFSVTEPKEPGESVVIRIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHVVEIEVTFDGVREMREAFTVHLKPDENMIAEMQLTKAIVYIEEMSSMADVTFPSVPQIVSLLMYDDTSKAKESAEPMSGYPVICITACNPKYSDYDKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMVTLDSIYFQPGSRVQCAARAVNTNGDEGLELMSPIVTISREEGLCQPRVPGVVGAEPFSAKLRYTGPEDADYTNLIKLTVTMPHIDGMLPVISTRELSNFELTLSPDGTRVGNHKCSNLLDYTEVKTHYGFLTDATKNPEIIGETYPYQYSLSIRGSTTLRFYRNLNLEACLWEFVSYYDMSELLADCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNDGIGSPPEAELQGSLYPTSMRIGDEGRLAVHFKTEAQFHGLFVLSHPASFTSSVIMSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLVPCTAPSHQEYRLPVTCNPREPVTFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYSPMNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNAKLAVDDPEAILLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVRSKDNANRGIGKRSVEYHSLVSQGKPQSTTKSRKKREIRSTPSLAWEIGAENSRGTNIQHIALDRTKRQIPHGRAPPDGILPWELNSPSSAVSLVTVVGGTTVGLLTICLTVIAVLMCRGKESFRGKDAPKGSSSSEPMVPPQSHHNDSSEV

Target ORF information:

RefSeq Version	NM_207361.6
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix 2 (FREM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207361.6

ORF Insert Sequence:

ATGCACTCAG CCGGGACTCC CGGGTTATCC TCGCGCCGGA CAGGCAACTC CACCAGCTTT 
CAACCAGGAC CGCCACCGCC GCCCCGGCTG CTGCTGCTGC TGCTGCTTCT CCTGTCACTG 
GTAAGCCGCG TCCCGGCACA GCCCGCTGCC TTCGGCAGGG CGTTGCTGTC CCCTGGTCTC 
GCGGGGGCTG CAGGGGTCCC TGCTGAGGAG GCCATAGTGC TGGCGAACCG CGGACTCCGG 
GTGCCTTTCG GCCGTGAAGT CTGGCTGGAT CCCCTGCATG ACCTGGTGTT GCAGGTGCAG 
CCCGGGGACC GCTGCGCGGT TTCGGTACTA GACAACGACG CACTGGCCCA GCGACCGGGC 
CGCCTGAGTC CCAAGCGCTT CCCGTGCGAC TTTGGCCCTG GCGAGGTGCG CTACTCTCAC 
CTGGGCGCGC GCAGCCCGTC TCGGGACCGC GTCCGGCTGC AGCTGCGCTA TGACGCGCCC 
GGAGGGGCAG TAGTGCTACC ACTGGTACTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACTC GGAACTTGCC TCTGGTCGTG GAAGAGCTGC TGGGGACCAG CAATGCCCTG 
GACGCGCGGA GCCTGGAGTT CGCCTTCCAG CCCGAGACAG AGGAGTGCCG CGTGGGCATC 
CTGTCCGGCT TGGGCGCGCT GCCTCGCTAT GGAGAACTCC TCCACTACCC GCAGGTCCCT 
GGAGGAGCCA GAGAGGGAGG CGCCCCGGAG ACTCTCCTGA TGGACTGCAA AGCTTTCCAG 
GAACTAGGCG TGCGCTATCG CCACACAGCC GCCAGTCGCT CACCAAACAG GGACTGGATA 
CCCATGGTGG TGGAGCTGCG TTCACGAGGG GCTCCTGTGG GCAGCCCTGC TTTGAAACGC 
GAGCACTTCC AGGTTCTGGT GAGGATCCGA GGAGGGGCCG AGAACACTGC ACCCAAGCCC 
AGTTTCGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TACTGACGGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGTCT CCCTCTGACC TGTTGATCTT CAACCTTACT 
TCTCCATTCC AGCCTGGCCA GGGCTACTTG GTGAGCACCG ATGATCGCAG CCTGCCCCTT 
TCCTCCTTCA CTCAGAGGGA TCTGCGGCTC CTGAAGATTG CCTACCAGCC CCCTTCTGAA 
GACTCTGACC AGGAGCGCCT CTTTGAACTG GAATTGGAGG TAGTGGATCT AGAAGGAGCA 
GCTTCAGACC CTTTTGCCTT CATGGTAGTG GTGAAGCCCA TGAACACAAT GGCTCCGGTG 
GTCACCCGGA ATACCGGTCT TATTCTCTAT GAGGGTCAGT CTCGGCCCCT CACAGGCCCT 
GCAGGCAGTG GTCCGCAAAA CTTGGTCATC AGCGATGAGG ATGACCTAGA AGCAGTGCGG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CACCTTGTCA TTCTGGGTGC TTCCAGTGGC 
AGCTCTGCTC CCAAGAGCTT TACAGTGGCT GAGCTGGCAG CCGGCCAGGT GGTCTACCAG 
CATGATGACA GAGACGGCTC GCTGAGCGAC AACCTGGTGC TTCGCATGGT GGATGGAGGA 
GGCAGGCACC AGGTACAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCTGTTCTCA ATGCCAACAC GGGGCTGACA CTGGCAGAGG GTGAAACAGT GCCCATCCTG 
CCCCTTTCCC TGAGTGCAAC TGACATGGAT TCAGATGATT CTCTGCTGCT TTTTGTGCTG 
GAGTCACCCT TCTTAACTAC GGGGCATCTG CTTCTCCGCC AAACTCACCC TCCCCATGAG 
AAGCAGGAAC TTCTCAGAGG CCTTTGGAGG AAGGAGGGGG CATTTTATGA GCGAACAGTG 
ACAGAGTGGC AGCAGCAGGA CATAACAGAG GGCAGGCTGT TCTATAGACA CTCTGGGCCC 
CATAGTCCTG GGCCAGTCAC AGACCAGTTC ACATTTAGAG TCCAGGATAA CCATGACCCT 
CCTAATCAGT CCGGGCTACA GCGGTTTGTG ATTCGTATCC ATCCTGTGGA TCGCCTCCCT 
CCGGAGCTGG GCAGTGGCTG TCCCCTTCGT ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTGCG CTACACTGAC CTGGACACAG ATGACCGAGA ACTACGTTAC 
ACAGTGACTC AGCCCCCCAC AGACACAGAC GAAAATCACC TGCCAGCCCC ACTGGGTACC 
TTGGTCTTGA CTGACAACCC CTCAGTCGTG GTGACCCATT TTACCCAAGC CCAGATCAAC 
CATCATAAAA TTGCTTACAG ACCCCCGGGT CAAGAACTGG GCGTGGCTAC TCGAGTGGCC 
CAGTTCCAGT TCCAGGTGGA AGACCGAGCT GGGAATGTGG CTCCAGGTAC CTTTACCCTT 
TACTTGCATC CCGTGGACAA CCAGCCACCT GAGATCCTCA ACACCGGCTT CACTATTCAG 
GAGAAGGGTC ACCACATCCT GAGTGAGACA GAGTTGCACG TGAATGATGT AGACACTGAT 
GTTGCCCATA TCTCTTTCAC TCTCACTCAG GCACCCAAAC ATGGCCACAT GAGAGTGTCT 
GGACAGATCC TGCATGTAGG GGGTCTCTTC CACTTGGAGG ACATAAAACA GGGCCGAGTT 
TCCTATGCCC ATAATGGGGA CAAGTCCCTG ACTGATAGCT GCTCCTTGGA AGTCAGTGAC 
AGACATCATG TGGTGCCCAT CACTCTCAGA GTAAATGTCC GGCCAGTGGA TGATGAAGTG 
CCCATACTGA GCCATCCTAC TGGCACTCTG GAGTCCTATC TAGATGTCTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATTAAG GGGACCAATG AGGAAACTGA TGACTTGATG 
TTGACTTTCC TCTTGGAAGA TCCACCTTTG TATGGGGAAA TCTTGGTCAA TGGCATTCCA 
GCAGAGCAGT TTACTCAAAG GGACATCTTG GAGGGCTCTG TTGTATATAC CCACACCAGT 
GGTGAGATAG GCCTATTGCC TAAAGCGGAT TCTTTTAACC TGAGTCTGTC AGATATGTCT 
CAAGAATGGA GAATTGGTGG CAATACTATC CAAGGAGTTA CTATATGGGT GACCATCCTG 
CCTGTTGATA GCCAGGCCCC AGAAATCTTT GTAGGTGAAC AGTTGATAGT AATGGAAGGT 
GATAAAAGTG TTATAACATC AGTGCATATA AGTGCTGAAG ATGTCGACTC CCTGAATGAT 
GACATCTTGT GCACTATAGT TATTCAGCCT ACTTCAGGTT ATGTTGAAAA CATTTCTCCA 
GCACCAGGCT CTGAGAAATC AAGAGCAGGG ATTGCCATAA GTGCTTTCAA CTTGAAAGAT 
CTCAGGCAGG GCCACATAAA CTATGTCCAG AGTGTCCATA AAGGGGTGGA ACCTGTGGAG 
GACCGATTTG TATTTCGTTG TTCTGATGGC ATTAACTTTT CAGAGAGACA GTTCTTCCCC 
ATTGTAATCA TTCCCACCAA TGATGAACAG CCAGAGATGT TTATGAGAGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT AATTGATACA CCCATTCTCA ATGCTGCTGA TGCTGATGTT 
CCCCTGGATG ATTTAACTTT CACTATTACC CAATTCCCCA CTCATGGTCA CATCATGAAT 
CAGCTGATAA ATGGCACGGT TTTGGTCGAA AGCTTCACCT TGGATCAGAT CATAGAGAGT 
TCCAGCATTA TTTATGAGCA TGATGACTCC GAGACCCAGG AAGACAGTTT TGTGATTAAA 
CTAACAGATG GGAAGCACTC TGTGGAAAAG ACGGTCCTCA TTATAGTTAT CCCTGTTGAT 
GATGAGACGC CCAGAATGAC TATCAATAAT GGACTAGAAA TAGAAATTGG GGATACCAAG 
ATTATCAACA ACAAAATATT AATGGCAACA GATTTAGATT CAGAAGACAA ATCTTTGGTT 
TATATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACGAAAACC TACTGGTGCC 
TTTGAAAATA TCACACTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAACTTAATT 
CAGTATGTCC ATTTGGGGCA AGAGGGCATT CGGGACCTAA TTAAATTTGA TGTGACTGAT 
GGAATAAATC CCCTCATAGA TCGTTACTTT TATGTGTCCA TCGGGAGCAT TGACATTGTC 
TTCCCTGATG TGATAAGTAA GGGAGTGTCC TTGAAAGAAG GTGGCAAAGT CACTCTTACA 
ACAGACCTAC TAAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTGGT TTTTACCATC 
ACCAGGGCTC CCATGCGAGG TCACCTGGAA TGCACGGATC AGCCTGGTGT GTCCATCACG 
TCTTTCACTC AGCTGCAACT GGCTGGAAAC AAAATCTACT ACATCCACAC AGCTGATGAT 
GAAGTGAAAA TGGACAGTTT TGAGTTTCAA GTCACCGATG GACGTAACCC TGTCTTTCGG 
ACATTCCGTA TCTCCATTAG CGATGTGGAC AATAAAAAGC CAGTGGTCAC CATCCACAAG 
CTGGTTGTCA GTGAAAGTGA AAACAAGCTG ATTACTCCTT TTGAGCTCAC TGTCGAAGAC 
AGAGATACTC CTGACAAGCT CCTGAAATTC ACTATCACCC AGGTGCCTAT TCATGGCCAT 
CTCCTATTCA ACAATACCAG ACCTGTCATG GTTTTTACCA AGCAAGACTT GAATGAAAAC 
TTAATCAGCT ACAAACATGA TGGCACTGAG TCAAGTGAAG ATAGCTTCTC CTTCACAGTG 
ACTGATGGCA CCCATACAGA CTTCTATGTT TTTCCTGATA CGGTGTTTGA AACAAGGAGA 
CCCCAAGTGA TGAAGATCCA GGTCTTGGCT GTTGACAACA GTGTCCCCCA AATCGCAGTG 
AATAAGGGGG CCTCTACACT TCGCACTCTA GCCACTGGCC ACTTGGGGTT CATGATCACA 
AGCAAAATAT TGAAAGTGGA GGACAGAGAC AGCTTACACA TTTCTCTTAG ATTTATCGTG 
ACAGAGGCCC CTCAACATGG ATATCTTCTC AACCTGGACA AAGGCAACCA CAGCATCACT 
CAGTTCACAC AAGCTGACAT TGATGACATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCACAA GTGATATGTT CTATTTTGCA GTTGAAGATG GTGGTGGAAA CAAGTTAACG 
TACCAGAATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACCTGGTC 
AATGAGGACT CCAAATTTCT AGATGTTGTT CTTAAACGTA GAGGTTACTT GGGAGAAACT 
TCTTTTATAA GTATTGGCAC AAGAGACAGA ACTGCAGAAA AAGACAAAGA CTTCAAGGGC 
AAAGCACAGA AACAAGTGCA GTTCAACCCA GGCCAGACCA GGGCCACATG GCGAGTGCGG 
ATCCTGAGTG ATGGGGAGCA TGAGCAGTCT GAAACCTTTC AGGTGGTACT CTCAGAGCCC 
GTGCTGGCTG CCTTGGAATT CCCCACAGTC GCCACTGTTG AGATCGTTGA TCCAGGAGAT 
GAGCCAACTG TGTTTATTCC CCAGTCCAAA TACTCCGTTG AAGAAGATGT TGGTGAGCTG 
TTCATTCCCA TCAGGAGGAG CGGAGATGTG AGCCAGGAGT TGATGGTGGT CTGTTATACC 
CAACAAGGAA CAGCAACTGG AACTGTGCCG ACTTCCGTGT TGTCTTACTC TGATTACATA 
TCCAGGCCTG AGGACCACAC CAGTGTTGTC CGCTTTGACA AAGATGAACG GGAGAAACTG 
TGTCGGATAG TCATAATTGA TGACTCTTTG TACGAGGAGG AGGAAACCTT CCATGTCCTT 
CTGAGCATGC CCATGGGGGG AAGAATCGGA TCAGAGTTCC CAGGGGCTCA AGTTACAATC 
GTTCCTGACA AAGATGATGA ACCCATCTTT TACTTCGGTG ATGTGGAATA CTCTGTGGAT 
GAGAGTGCTG GCTATGTGGA AGTGCAGGTG TGGAGAACGG GCACTGACCT GTCCAAGTCT 
TCTAGTGTCA CAGTGAGGTC TCGGAAAACA GATCCTCCCT CTGCAGATGC TGGAACAGAC 
TATGTGGGCA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA GCCTGTTCGT 
GTTGTCATTC TGGATGACCT TGGACAACCA GCGCTGGAGG GAATTGAGAA ATTTGAACTG 
GTGCTTCGCA TGCCTATGAA CGCAGCCCTT GGCGAGCCCA GCAAAGCCAC AGTGTCCATA 
AATGACTCTG TCTCCGATTT GCCTAAGATG CAATTCAAAG AACGAATATA TACTGGCAGC 
GAAAGTGATG GGCAGATAGT TACAATGATC CATAGGACTG GGGATGTCCA GTACAGATCT 
TCAGTGAGAT GCTACACCCG GCAGGGGTCT GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCAAACACTG ATACCTCCAT CATCACATTC CTCCCTGGTG AGACAGAAAA GCCCTGCATT 
CTTGAGCTGA TGGACGATGT GCTCTATGAG GAGGTAGAGG AGCTCCGCCT GGTACTCGGC 
ACTCCACAAA GCAACTCTCC CTTTGGGGCT GCAGTTGGTG AACAAAATGA AACTCTCATA 
AGGATCCGAG ATGATGCTGA TAAGACTGTT ATTAAATTTG GAGAAACCAA ATTTAGTGTC 
ACTGAACCCA AAGAACCTGG AGAGTCGGTG GTTATAAGAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AGGTTTCCAT TGTGAGAGTC CACACCAAGG ATGGCTCGGC CACCTCTGGA 
GAAGACTACC ACCCTGTGTC AGAAGAAATT GAGTTTAAGG AAGGGGAAAC CCAGCACGTG 
GTTGAAATCG AAGTTACCTT TGACGGGGTG AGAGAGATGA GAGAGGCCTT CACTGTTCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ATGCAGTTGA CGAAAGCCAT TGTGTACATA 
GAAGAAATGA GCAGCATGGC AGATGTCACT TTTCCTTCTG TCCCTCAAAT TGTATCCCTG 
TTGATGTATG ACGACACTTC CAAAGCTAAG GAGAGTGCTG AACCCATGTC TGGCTATCCT 
GTCATCTGTA TCACAGCTTG CAACCCCAAA TATTCAGACT ACGATAAAAC AGGCTCTATC 
TGTGCAAGTG AGAACATCAA TGACACTTTG ACGCGGTACC GGTGGCTGAT TAGTGCACCT 
GCGGGCCCTG ACGGTGTGAC CAGCCCTATG AGAGAAGTGG ACTTCGACAC CTTTTTTACG 
TCATCCAAGA TGGTCACACT GGACTCCATA TACTTTCAGC CTGGCTCCCG GGTACAGTGC 
GCAGCTCGTG CTGTGAACAC CAATGGGGAT GAAGGCCTGG AGCTCATGAG CCCTATTGTA 
ACCATCAGCA GAGAAGAAGG TCTTTGTCAG CCCCGTGTAC CTGGGGTTGT TGGAGCAGAG 
CCGTTCTCAG CTAAATTGCG CTACACAGGC CCTGAGGATG CAGACTACAC AAACCTTATC 
AAGCTCACTG TCACAATGCC ACACATAGAT GGCATGCTCC CCGTGATCTC CACTAGAGAG 
CTTTCCAACT TTGAGCTCAC CCTCAGCCCT GATGGCACAA GAGTTGGAAA CCACAAGTGC 
TCCAACCTCC TGGATTATAC TGAAGTGAAG ACTCATTATG GTTTCTTGAC TGATGCTACC 
AAAAATCCAG AAATAATTGG AGAGACATAT CCTTACCAGT ACAGCTTGTC CATCAGAGGT 
TCCACTACCT TGCGCTTCTA CCGGAACCTG AACCTAGAGG CCTGTTTATG GGAGTTCGTT 
AGCTACTATG ACATGTCAGA ACTCCTTGCT GACTGTGGTG GCACCATTGG AACAGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTATGTG ACCCTTCGAG TCCCTCTGTA TGTTTCCTAC 
GTGTTCCATT CCCCCGTGGG GGTAGGAGGC TGGCAGCATT TTGACTTGAA GTCAGAGCTT 
CGTCTAACTT TTGTGTACGA CACCGCCATC TTGTGGAATG ATGGAATTGG CAGCCCCCCA 
GAGGCTGAAC TTCAAGGTTC TCTCTATCCA ACCAGCATGC GCATCGGTGA TGAGGGGCGC 
TTGGCCGTGC ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TATTTGTGCT GTCACATCCC 
GCATCCTTTA CAAGCTCAGT GATCATGTCA GCTGATCATC CAGGCCTGAC ATTTTCCCTC 
CGCCTCATAA GGAGTGAACC AACCTATAAC CAGCCAGTAC AGCAGTGGAG CTTTGTCTCT 
GACTTTGCCG TACGTGACTA CTCAGGGACC TATACTGTGA AGCTGGTGCC ATGCACTGCC 
CCATCACATC AGGAATACCG CCTGCCAGTC ACCTGCAACC CCAGAGAACC TGTCACCTTT 
GACCTTGACA TCCGATTCCA ACAGGTCAGT GATCCAGTGG CTGCTGAGTT TAGCTTGAAC 
ACCCAAATGT ACCTGCTCTC TAAGAAGAGT CTCTGGTTGT CTGATGGATC CATGGGATTC 
GGGCAAGAGA GTGATGTTGC TTTTGCAGAA GGTGATATAA TTTATGGTCG TGTCATGGTG 
GATCCTGTCC AGAATCTGGG TGACTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTATGC 
ACTGGAGCTG ATGGCTATGT TCCCAAGTAT AGTCCAATGA ATGCAGAATA TGGCTGCTTA 
GCCGACTCTC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA GCCAGAGACA 
CAAGCGACCA GTTTTGGAAA TGTCCTATTT AATGCCAAAC TAGCAGTGGA TGACCCTGAA 
GCCATTCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTCG CTCTAGGCCG AGAATGGTAT ATACATACGA TCTATACAGT GAGATCGAAA 
GACAATGCCA ATCGAGGTAT TGGCAAAAGA AGTGTGGAGT ACCATTCTCT GGTGAGTCAA 
GGAAAGCCCC AATCCACCAC CAAGAGCCGG AAGAAGAGAG AGATCAGGAG CACACCCTCA 
CTGGCATGGG AGATTGGTGC TGAAAACAGT CGAGGAACAA ACATCCAGCA CATTGCCCTG 
GACCGCACCA AGAGGCAGAT CCCCCATGGG AGAGCACCTC CAGATGGCAT CCTCCCCTGG 
GAGCTCAACA GCCCCAGCTC TGCAGTCAGC CTGGTCACTG TGGTGGGAGG CACCACGGTA 
GGGTTACTCA CCATCTGCCT CACTGTCATT GCAGTGCTGA TGTGCAGGGG CAAGGAAAGT 
TTCAGGGGGA AGGATGCCCC GAAAGGCTCC AGCAGCAGTG AGCCCATGGT GCCCCCACAG 
AGCCATCACA ATGACAGCTC AGAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FREM2 ( NM_207361.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_997244.4 Homo sapiens FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

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CloneID	OHu28448
Clone ID Related Accession (Same CDS sequence)	NM_207361.6 , NM_207361.5
Accession Version	NM_207361.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-17
Organism	Homo sapiens(human)
Product	FRAS1-related extracellular matrix protein 2 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN291155.1, BN000687.1, KF455770.1, AL354819.15 and BX640686.1. This sequence is a reference standard in the RefSeqGene project. On Apr 19, 2014 this sequence version replaced NM_207361.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. ##Evidence-Data-START## Transcript exon combination :: BN000687.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCACTCAG CCGGGACTCC CGGGTTATCC TCGCGCCGGA CAGGCAACTC CACCAGCTTT 
CAACCAGGAC CGCCACCGCC GCCCCGGCTG CTGCTGCTGC TGCTGCTTCT CCTGTCACTG 
GTAAGCCGCG TCCCGGCACA GCCCGCTGCC TTCGGCAGGG CGTTGCTGTC CCCTGGTCTC 
GCGGGGGCTG CAGGGGTCCC TGCTGAGGAG GCCATAGTGC TGGCGAACCG CGGACTCCGG 
GTGCCTTTCG GCCGTGAAGT CTGGCTGGAT CCCCTGCATG ACCTGGTGTT GCAGGTGCAG 
CCCGGGGACC GCTGCGCGGT TTCGGTACTA GACAACGACG CACTGGCCCA GCGACCGGGC 
CGCCTGAGTC CCAAGCGCTT CCCGTGCGAC TTTGGCCCTG GCGAGGTGCG CTACTCTCAC 
CTGGGCGCGC GCAGCCCGTC TCGGGACCGC GTCCGGCTGC AGCTGCGCTA TGACGCGCCC 
GGAGGGGCAG TAGTGCTACC ACTGGTACTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACTC GGAACTTGCC TCTGGTCGTG GAAGAGCTGC TGGGGACCAG CAATGCCCTG 
GACGCGCGGA GCCTGGAGTT CGCCTTCCAG CCCGAGACAG AGGAGTGCCG CGTGGGCATC 
CTGTCCGGCT TGGGCGCGCT GCCTCGCTAT GGAGAACTCC TCCACTACCC GCAGGTCCCT 
GGAGGAGCCA GAGAGGGAGG CGCCCCGGAG ACTCTCCTGA TGGACTGCAA AGCTTTCCAG 
GAACTAGGCG TGCGCTATCG CCACACAGCC GCCAGTCGCT CACCAAACAG GGACTGGATA 
CCCATGGTGG TGGAGCTGCG TTCACGAGGG GCTCCTGTGG GCAGCCCTGC TTTGAAACGC 
GAGCACTTCC AGGTTCTGGT GAGGATCCGA GGAGGGGCCG AGAACACTGC ACCCAAGCCC 
AGTTTCGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TACTGACGGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGTCT CCCTCTGACC TGTTGATCTT CAACCTTACT 
TCTCCATTCC AGCCTGGCCA GGGCTACTTG GTGAGCACCG ATGATCGCAG CCTGCCCCTT 
TCCTCCTTCA CTCAGAGGGA TCTGCGGCTC CTGAAGATTG CCTACCAGCC CCCTTCTGAA 
GACTCTGACC AGGAGCGCCT CTTTGAACTG GAATTGGAGG TAGTGGATCT AGAAGGAGCA 
GCTTCAGACC CTTTTGCCTT CATGGTAGTG GTGAAGCCCA TGAACACAAT GGCTCCGGTG 
GTCACCCGGA ATACCGGTCT TATTCTCTAT GAGGGTCAGT CTCGGCCCCT CACAGGCCCT 
GCAGGCAGTG GTCCGCAAAA CTTGGTCATC AGCGATGAGG ATGACCTAGA AGCAGTGCGG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CACCTTGTCA TTCTGGGTGC TTCCAGTGGC 
AGCTCTGCTC CCAAGAGCTT TACAGTGGCT GAGCTGGCAG CCGGCCAGGT GGTCTACCAG 
CATGATGACA GAGACGGCTC GCTGAGCGAC AACCTGGTGC TTCGCATGGT GGATGGAGGA 
GGCAGGCACC AGGTACAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCTGTTCTCA ATGCCAACAC GGGGCTGACA CTGGCAGAGG GTGAAACAGT GCCCATCCTG 
CCCCTTTCCC TGAGTGCAAC TGACATGGAT TCAGATGATT CTCTGCTGCT TTTTGTGCTG 
GAGTCACCCT TCTTAACTAC GGGGCATCTG CTTCTCCGCC AAACTCACCC TCCCCATGAG 
AAGCAGGAAC TTCTCAGAGG CCTTTGGAGG AAGGAGGGGG CATTTTATGA GCGAACAGTG 
ACAGAGTGGC AGCAGCAGGA CATAACAGAG GGCAGGCTGT TCTATAGACA CTCTGGGCCC 
CATAGTCCTG GGCCAGTCAC AGACCAGTTC ACATTTAGAG TCCAGGATAA CCATGACCCT 
CCTAATCAGT CCGGGCTACA GCGGTTTGTG ATTCGTATCC ATCCTGTGGA TCGCCTCCCT 
CCGGAGCTGG GCAGTGGCTG TCCCCTTCGT ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTGCG CTACACTGAC CTGGACACAG ATGACCGAGA ACTACGTTAC 
ACAGTGACTC AGCCCCCCAC AGACACAGAC GAAAATCACC TGCCAGCCCC ACTGGGTACC 
TTGGTCTTGA CTGACAACCC CTCAGTCGTG GTGACCCATT TTACCCAAGC CCAGATCAAC 
CATCATAAAA TTGCTTACAG ACCCCCGGGT CAAGAACTGG GCGTGGCTAC TCGAGTGGCC 
CAGTTCCAGT TCCAGGTGGA AGACCGAGCT GGGAATGTGG CTCCAGGTAC CTTTACCCTT 
TACTTGCATC CCGTGGACAA CCAGCCACCT GAGATCCTCA ACACCGGCTT CACTATTCAG 
GAGAAGGGTC ACCACATCCT GAGTGAGACA GAGTTGCACG TGAATGATGT AGACACTGAT 
GTTGCCCATA TCTCTTTCAC TCTCACTCAG GCACCCAAAC ATGGCCACAT GAGAGTGTCT 
GGACAGATCC TGCATGTAGG GGGTCTCTTC CACTTGGAGG ACATAAAACA GGGCCGAGTT 
TCCTATGCCC ATAATGGGGA CAAGTCCCTG ACTGATAGCT GCTCCTTGGA AGTCAGTGAC 
AGACATCATG TGGTGCCCAT CACTCTCAGA GTAAATGTCC GGCCAGTGGA TGATGAAGTG 
CCCATACTGA GCCATCCTAC TGGCACTCTG GAGTCCTATC TAGATGTCTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATTAAG GGGACCAATG AGGAAACTGA TGACTTGATG 
TTGACTTTCC TCTTGGAAGA TCCACCTTTG TATGGGGAAA TCTTGGTCAA TGGCATTCCA 
GCAGAGCAGT TTACTCAAAG GGACATCTTG GAGGGCTCTG TTGTATATAC CCACACCAGT 
GGTGAGATAG GCCTATTGCC TAAAGCGGAT TCTTTTAACC TGAGTCTGTC AGATATGTCT 
CAAGAATGGA GAATTGGTGG CAATACTATC CAAGGAGTTA CTATATGGGT GACCATCCTG 
CCTGTTGATA GCCAGGCCCC AGAAATCTTT GTAGGTGAAC AGTTGATAGT AATGGAAGGT 
GATAAAAGTG TTATAACATC AGTGCATATA AGTGCTGAAG ATGTCGACTC CCTGAATGAT 
GACATCTTGT GCACTATAGT TATTCAGCCT ACTTCAGGTT ATGTTGAAAA CATTTCTCCA 
GCACCAGGCT CTGAGAAATC AAGAGCAGGG ATTGCCATAA GTGCTTTCAA CTTGAAAGAT 
CTCAGGCAGG GCCACATAAA CTATGTCCAG AGTGTCCATA AAGGGGTGGA ACCTGTGGAG 
GACCGATTTG TATTTCGTTG TTCTGATGGC ATTAACTTTT CAGAGAGACA GTTCTTCCCC 
ATTGTAATCA TTCCCACCAA TGATGAACAG CCAGAGATGT TTATGAGAGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT AATTGATACA CCCATTCTCA ATGCTGCTGA TGCTGATGTT 
CCCCTGGATG ATTTAACTTT CACTATTACC CAATTCCCCA CTCATGGTCA CATCATGAAT 
CAGCTGATAA ATGGCACGGT TTTGGTCGAA AGCTTCACCT TGGATCAGAT CATAGAGAGT 
TCCAGCATTA TTTATGAGCA TGATGACTCC GAGACCCAGG AAGACAGTTT TGTGATTAAA 
CTAACAGATG GGAAGCACTC TGTGGAAAAG ACGGTCCTCA TTATAGTTAT CCCTGTTGAT 
GATGAGACGC CCAGAATGAC TATCAATAAT GGACTAGAAA TAGAAATTGG GGATACCAAG 
ATTATCAACA ACAAAATATT AATGGCAACA GATTTAGATT CAGAAGACAA ATCTTTGGTT 
TATATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACGAAAACC TACTGGTGCC 
TTTGAAAATA TCACACTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAACTTAATT 
CAGTATGTCC ATTTGGGGCA AGAGGGCATT CGGGACCTAA TTAAATTTGA TGTGACTGAT 
GGAATAAATC CCCTCATAGA TCGTTACTTT TATGTGTCCA TCGGGAGCAT TGACATTGTC 
TTCCCTGATG TGATAAGTAA GGGAGTGTCC TTGAAAGAAG GTGGCAAAGT CACTCTTACA 
ACAGACCTAC TAAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTGGT TTTTACCATC 
ACCAGGGCTC CCATGCGAGG TCACCTGGAA TGCACGGATC AGCCTGGTGT GTCCATCACG 
TCTTTCACTC AGCTGCAACT GGCTGGAAAC AAAATCTACT ACATCCACAC AGCTGATGAT 
GAAGTGAAAA TGGACAGTTT TGAGTTTCAA GTCACCGATG GACGTAACCC TGTCTTTCGG 
ACATTCCGTA TCTCCATTAG CGATGTGGAC AATAAAAAGC CAGTGGTCAC CATCCACAAG 
CTGGTTGTCA GTGAAAGTGA AAACAAGCTG ATTACTCCTT TTGAGCTCAC TGTCGAAGAC 
AGAGATACTC CTGACAAGCT CCTGAAATTC ACTATCACCC AGGTGCCTAT TCATGGCCAT 
CTCCTATTCA ACAATACCAG ACCTGTCATG GTTTTTACCA AGCAAGACTT GAATGAAAAC 
TTAATCAGCT ACAAACATGA TGGCACTGAG TCAAGTGAAG ATAGCTTCTC CTTCACAGTG 
ACTGATGGCA CCCATACAGA CTTCTATGTT TTTCCTGATA CGGTGTTTGA AACAAGGAGA 
CCCCAAGTGA TGAAGATCCA GGTCTTGGCT GTTGACAACA GTGTCCCCCA AATCGCAGTG 
AATAAGGGGG CCTCTACACT TCGCACTCTA GCCACTGGCC ACTTGGGGTT CATGATCACA 
AGCAAAATAT TGAAAGTGGA GGACAGAGAC AGCTTACACA TTTCTCTTAG ATTTATCGTG 
ACAGAGGCCC CTCAACATGG ATATCTTCTC AACCTGGACA AAGGCAACCA CAGCATCACT 
CAGTTCACAC AAGCTGACAT TGATGACATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCACAA GTGATATGTT CTATTTTGCA GTTGAAGATG GTGGTGGAAA CAAGTTAACG 
TACCAGAATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACCTGGTC 
AATGAGGACT CCAAATTTCT AGATGTTGTT CTTAAACGTA GAGGTTACTT GGGAGAAACT 
TCTTTTATAA GTATTGGCAC AAGAGACAGA ACTGCAGAAA AAGACAAAGA CTTCAAGGGC 
AAAGCACAGA AACAAGTGCA GTTCAACCCA GGCCAGACCA GGGCCACATG GCGAGTGCGG 
ATCCTGAGTG ATGGGGAGCA TGAGCAGTCT GAAACCTTTC AGGTGGTACT CTCAGAGCCC 
GTGCTGGCTG CCTTGGAATT CCCCACAGTC GCCACTGTTG AGATCGTTGA TCCAGGAGAT 
GAGCCAACTG TGTTTATTCC CCAGTCCAAA TACTCCGTTG AAGAAGATGT TGGTGAGCTG 
TTCATTCCCA TCAGGAGGAG CGGAGATGTG AGCCAGGAGT TGATGGTGGT CTGTTATACC 
CAACAAGGAA CAGCAACTGG AACTGTGCCG ACTTCCGTGT TGTCTTACTC TGATTACATA 
TCCAGGCCTG AGGACCACAC CAGTGTTGTC CGCTTTGACA AAGATGAACG GGAGAAACTG 
TGTCGGATAG TCATAATTGA TGACTCTTTG TACGAGGAGG AGGAAACCTT CCATGTCCTT 
CTGAGCATGC CCATGGGGGG AAGAATCGGA TCAGAGTTCC CAGGGGCTCA AGTTACAATC 
GTTCCTGACA AAGATGATGA ACCCATCTTT TACTTCGGTG ATGTGGAATA CTCTGTGGAT 
GAGAGTGCTG GCTATGTGGA AGTGCAGGTG TGGAGAACGG GCACTGACCT GTCCAAGTCT 
TCTAGTGTCA CAGTGAGGTC TCGGAAAACA GATCCTCCCT CTGCAGATGC TGGAACAGAC 
TATGTGGGCA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA GCCTGTTCGT 
GTTGTCATTC TGGATGACCT TGGACAACCA GCGCTGGAGG GAATTGAGAA ATTTGAACTG 
GTGCTTCGCA TGCCTATGAA CGCAGCCCTT GGCGAGCCCA GCAAAGCCAC AGTGTCCATA 
AATGACTCTG TCTCCGATTT GCCTAAGATG CAATTCAAAG AACGAATATA TACTGGCAGC 
GAAAGTGATG GGCAGATAGT TACAATGATC CATAGGACTG GGGATGTCCA GTACAGATCT 
TCAGTGAGAT GCTACACCCG GCAGGGGTCT GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCAAACACTG ATACCTCCAT CATCACATTC CTCCCTGGTG AGACAGAAAA GCCCTGCATT 
CTTGAGCTGA TGGACGATGT GCTCTATGAG GAGGTAGAGG AGCTCCGCCT GGTACTCGGC 
ACTCCACAAA GCAACTCTCC CTTTGGGGCT GCAGTTGGTG AACAAAATGA AACTCTCATA 
AGGATCCGAG ATGATGCTGA TAAGACTGTT ATTAAATTTG GAGAAACCAA ATTTAGTGTC 
ACTGAACCCA AAGAACCTGG AGAGTCGGTG GTTATAAGAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AGGTTTCCAT TGTGAGAGTC CACACCAAGG ATGGCTCGGC CACCTCTGGA 
GAAGACTACC ACCCTGTGTC AGAAGAAATT GAGTTTAAGG AAGGGGAAAC CCAGCACGTG 
GTTGAAATCG AAGTTACCTT TGACGGGGTG AGAGAGATGA GAGAGGCCTT CACTGTTCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ATGCAGTTGA CGAAAGCCAT TGTGTACATA 
GAAGAAATGA GCAGCATGGC AGATGTCACT TTTCCTTCTG TCCCTCAAAT TGTATCCCTG 
TTGATGTATG ACGACACTTC CAAAGCTAAG GAGAGTGCTG AACCCATGTC TGGCTATCCT 
GTCATCTGTA TCACAGCTTG CAACCCCAAA TATTCAGACT ACGATAAAAC AGGCTCTATC 
TGTGCAAGTG AGAACATCAA TGACACTTTG ACGCGGTACC GGTGGCTGAT TAGTGCACCT 
GCGGGCCCTG ACGGTGTGAC CAGCCCTATG AGAGAAGTGG ACTTCGACAC CTTTTTTACG 
TCATCCAAGA TGGTCACACT GGACTCCATA TACTTTCAGC CTGGCTCCCG GGTACAGTGC 
GCAGCTCGTG CTGTGAACAC CAATGGGGAT GAAGGCCTGG AGCTCATGAG CCCTATTGTA 
ACCATCAGCA GAGAAGAAGG TCTTTGTCAG CCCCGTGTAC CTGGGGTTGT TGGAGCAGAG 
CCGTTCTCAG CTAAATTGCG CTACACAGGC CCTGAGGATG CAGACTACAC AAACCTTATC 
AAGCTCACTG TCACAATGCC ACACATAGAT GGCATGCTCC CCGTGATCTC CACTAGAGAG 
CTTTCCAACT TTGAGCTCAC CCTCAGCCCT GATGGCACAA GAGTTGGAAA CCACAAGTGC 
TCCAACCTCC TGGATTATAC TGAAGTGAAG ACTCATTATG GTTTCTTGAC TGATGCTACC 
AAAAATCCAG AAATAATTGG AGAGACATAT CCTTACCAGT ACAGCTTGTC CATCAGAGGT 
TCCACTACCT TGCGCTTCTA CCGGAACCTG AACCTAGAGG CCTGTTTATG GGAGTTCGTT 
AGCTACTATG ACATGTCAGA ACTCCTTGCT GACTGTGGTG GCACCATTGG AACAGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTATGTG ACCCTTCGAG TCCCTCTGTA TGTTTCCTAC 
GTGTTCCATT CCCCCGTGGG GGTAGGAGGC TGGCAGCATT TTGACTTGAA GTCAGAGCTT 
CGTCTAACTT TTGTGTACGA CACCGCCATC TTGTGGAATG ATGGAATTGG CAGCCCCCCA 
GAGGCTGAAC TTCAAGGTTC TCTCTATCCA ACCAGCATGC GCATCGGTGA TGAGGGGCGC 
TTGGCCGTGC ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TATTTGTGCT GTCACATCCC 
GCATCCTTTA CAAGCTCAGT GATCATGTCA GCTGATCATC CAGGCCTGAC ATTTTCCCTC 
CGCCTCATAA GGAGTGAACC AACCTATAAC CAGCCAGTAC AGCAGTGGAG CTTTGTCTCT 
GACTTTGCCG TACGTGACTA CTCAGGGACC TATACTGTGA AGCTGGTGCC ATGCACTGCC 
CCATCACATC AGGAATACCG CCTGCCAGTC ACCTGCAACC CCAGAGAACC TGTCACCTTT 
GACCTTGACA TCCGATTCCA ACAGGTCAGT GATCCAGTGG CTGCTGAGTT TAGCTTGAAC 
ACCCAAATGT ACCTGCTCTC TAAGAAGAGT CTCTGGTTGT CTGATGGATC CATGGGATTC 
GGGCAAGAGA GTGATGTTGC TTTTGCAGAA GGTGATATAA TTTATGGTCG TGTCATGGTG 
GATCCTGTCC AGAATCTGGG TGACTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTATGC 
ACTGGAGCTG ATGGCTATGT TCCCAAGTAT AGTCCAATGA ATGCAGAATA TGGCTGCTTA 
GCCGACTCTC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA GCCAGAGACA 
CAAGCGACCA GTTTTGGAAA TGTCCTATTT AATGCCAAAC TAGCAGTGGA TGACCCTGAA 
GCCATTCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTCG CTCTAGGCCG AGAATGGTAT ATACATACGA TCTATACAGT GAGATCGAAA 
GACAATGCCA ATCGAGGTAT TGGCAAAAGA AGTGTGGAGT ACCATTCTCT GGTGAGTCAA 
GGAAAGCCCC AATCCACCAC CAAGAGCCGG AAGAAGAGAG AGATCAGGAG CACACCCTCA 
CTGGCATGGG AGATTGGTGC TGAAAACAGT CGAGGAACAA ACATCCAGCA CATTGCCCTG 
GACCGCACCA AGAGGCAGAT CCCCCATGGG AGAGCACCTC CAGATGGCAT CCTCCCCTGG 
GAGCTCAACA GCCCCAGCTC TGCAGTCAGC CTGGTCACTG TGGTGGGAGG CACCACGGTA 
GGGTTACTCA CCATCTGCCT CACTGTCATT GCAGTGCTGA TGTGCAGGGG CAAGGAAAGT 
TTCAGGGGGA AGGATGCCCC GAAAGGCTCC AGCAGCAGTG AGCCCATGGT GCCCCCACAG 
AGCCATCACA ATGACAGCTC AGAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997244.4
CDS	310..9819
Translation	MHSAGTPGLSSRRTGNSTSFQPGPPPPPRLLLLLLLLLSLVSRVPAQPAAFGRALLSPGLAGAAGVPAEEAIVLANRGLRVPFGREVWLDPLHDLVLQVQPGDRCAVSVLDNDALAQRPGRLSPKRFPCDFGPGEVRYSHLGARSPSRDRVRLQLRYDAPGGAVVLPLVLEVEVVFTQLEVVTRNLPLVVEELLGTSNALDARSLEFAFQPETEECRVGILSGLGALPRYGELLHYPQVPGGAREGGAPETLLMDCKAFQELGVRYRHTAASRSPNRDWIPMVVELRSRGAPVGSPALKREHFQVLVRIRGGAENTAPKPSFVAMMMMEVDQFVLTALTPDMLAAEDAESPSDLLIFNLTSPFQPGQGYLVSTDDRSLPLSSFTQRDLRLLKIAYQPPSEDSDQERLFELELEVVDLEGAASDPFAFMVVVKPMNTMAPVVTRNTGLILYEGQSRPLTGPAGSGPQNLVISDEDDLEAVRLEVVAGLRHGHLVILGASSGSSAPKSFTVAELAAGQVVYQHDDRDGSLSDNLVLRMVDGGGRHQVQFLFPITLVPVDDQPPVLNANTGLTLAEGETVPILPLSLSATDMDSDDSLLLFVLESPFLTTGHLLLRQTHPPHEKQELLRGLWRKEGAFYERTVTEWQQQDITEGRLFYRHSGPHSPGPVTDQFTFRVQDNHDPPNQSGLQRFVIRIHPVDRLPPELGSGCPLRMVVQESQLTPLRKKWLRYTDLDTDDRELRYTVTQPPTDTDENHLPAPLGTLVLTDNPSVVVTHFTQAQINHHKIAYRPPGQELGVATRVAQFQFQVEDRAGNVAPGTFTLYLHPVDNQPPEILNTGFTIQEKGHHILSETELHVNDVDTDVAHISFTLTQAPKHGHMRVSGQILHVGGLFHLEDIKQGRVSYAHNGDKSLTDSCSLEVSDRHHVVPITLRVNVRPVDDEVPILSHPTGTLESYLDVLENGATEITANVIKGTNEETDDLMLTFLLEDPPLYGEILVNGIPAEQFTQRDILEGSVVYTHTSGEIGLLPKADSFNLSLSDMSQEWRIGGNTIQGVTIWVTILPVDSQAPEIFVGEQLIVMEGDKSVITSVHISAEDVDSLNDDILCTIVIQPTSGYVENISPAPGSEKSRAGIAISAFNLKDLRQGHINYVQSVHKGVEPVEDRFVFRCSDGINFSERQFFPIVIIPTNDEQPEMFMREFMVMEGMSLVIDTPILNAADADVPLDDLTFTITQFPTHGHIMNQLINGTVLVESFTLDQIIESSSIIYEHDDSETQEDSFVIKLTDGKHSVEKTVLIIVIPVDDETPRMTINNGLEIEIGDTKIINNKILMATDLDSEDKSLVYIIRYGPGHGLLQRRKPTGAFENITLGMNFTQDEVDRNLIQYVHLGQEGIRDLIKFDVTDGINPLIDRYFYVSIGSIDIVFPDVISKGVSLKEGGKVTLTTDLLSTSDLNSPDENLVFTITRAPMRGHLECTDQPGVSITSFTQLQLAGNKIYYIHTADDEVKMDSFEFQVTDGRNPVFRTFRISISDVDNKKPVVTIHKLVVSESENKLITPFELTVEDRDTPDKLLKFTITQVPIHGHLLFNNTRPVMVFTKQDLNENLISYKHDGTESSEDSFSFTVTDGTHTDFYVFPDTVFETRRPQVMKIQVLAVDNSVPQIAVNKGASTLRTLATGHLGFMITSKILKVEDRDSLHISLRFIVTEAPQHGYLLNLDKGNHSITQFTQADIDDMKICYVLREGANATSDMFYFAVEDGGGNKLTYQNFRLNWAWISFEKEYYLVNEDSKFLDVVLKRRGYLGETSFISIGTRDRTAEKDKDFKGKAQKQVQFNPGQTRATWRVRILSDGEHEQSETFQVVLSEPVLAALEFPTVATVEIVDPGDEPTVFIPQSKYSVEEDVGELFIPIRRSGDVSQELMVVCYTQQGTATGTVPTSVLSYSDYISRPEDHTSVVRFDKDEREKLCRIVIIDDSLYEEEETFHVLLSMPMGGRIGSEFPGAQVTIVPDKDDEPIFYFGDVEYSVDESAGYVEVQVWRTGTDLSKSSSVTVRSRKTDPPSADAGTDYVGISRNLDFAPGVNMQPVRVVILDDLGQPALEGIEKFELVLRMPMNAALGEPSKATVSINDSVSDLPKMQFKERIYTGSESDGQIVTMIHRTGDVQYRSSVRCYTRQGSAQVMMDFEERPNTDTSIITFLPGETEKPCILELMDDVLYEEVEELRLVLGTPQSNSPFGAAVGEQNETLIRIRDDADKTVIKFGETKFSVTEPKEPGESVVIRIPVIRQGDTSKVSIVRVHTKDGSATSGEDYHPVSEEIEFKEGETQHVVEIEVTFDGVREMREAFTVHLKPDENMIAEMQLTKAIVYIEEMSSMADVTFPSVPQIVSLLMYDDTSKAKESAEPMSGYPVICITACNPKYSDYDKTGSICASENINDTLTRYRWLISAPAGPDGVTSPMREVDFDTFFTSSKMVTLDSIYFQPGSRVQCAARAVNTNGDEGLELMSPIVTISREEGLCQPRVPGVVGAEPFSAKLRYTGPEDADYTNLIKLTVTMPHIDGMLPVISTRELSNFELTLSPDGTRVGNHKCSNLLDYTEVKTHYGFLTDATKNPEIIGETYPYQYSLSIRGSTTLRFYRNLNLEACLWEFVSYYDMSELLADCGGTIGTDGQVLNLVQSYVTLRVPLYVSYVFHSPVGVGGWQHFDLKSELRLTFVYDTAILWNDGIGSPPEAELQGSLYPTSMRIGDEGRLAVHFKTEAQFHGLFVLSHPASFTSSVIMSADHPGLTFSLRLIRSEPTYNQPVQQWSFVSDFAVRDYSGTYTVKLVPCTAPSHQEYRLPVTCNPREPVTFDLDIRFQQVSDPVAAEFSLNTQMYLLSKKSLWLSDGSMGFGQESDVAFAEGDIIYGRVMVDPVQNLGDSFYCSIEKVFLCTGADGYVPKYSPMNAEYGCLADSPSLLYRFKIVDKAQPETQATSFGNVLFNAKLAVDDPEAILLVNQPGSDGFKVDSTPLFQVALGREWYIHTIYTVRSKDNANRGIGKRSVEYHSLVSQGKPQSTTKSRKKREIRSTPSLAWEIGAENSRGTNIQHIALDRTKRQIPHGRAPPDGILPWELNSPSSAVSLVTVVGGTTVGLLTICLTVIAVLMCRGKESFRGKDAPKGSSSSEPMVPPQSHHNDSSEV

Target ORF information:

RefSeq Version	NM_207361.5
Organism	Homo sapiens(human)
Definition	Homo sapiens FRAS1 related extracellular matrix protein 2 (FREM2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_207361.5

ORF Insert Sequence:

ATGCACTCAG CCGGGACTCC CGGGTTATCC TCGCGCCGGA CAGGCAACTC CACCAGCTTT 
CAACCAGGAC CGCCACCGCC GCCCCGGCTG CTGCTGCTGC TGCTGCTTCT CCTGTCACTG 
GTAAGCCGCG TCCCGGCACA GCCCGCTGCC TTCGGCAGGG CGTTGCTGTC CCCTGGTCTC 
GCGGGGGCTG CAGGGGTCCC TGCTGAGGAG GCCATAGTGC TGGCGAACCG CGGACTCCGG 
GTGCCTTTCG GCCGTGAAGT CTGGCTGGAT CCCCTGCATG ACCTGGTGTT GCAGGTGCAG 
CCCGGGGACC GCTGCGCGGT TTCGGTACTA GACAACGACG CACTGGCCCA GCGACCGGGC 
CGCCTGAGTC CCAAGCGCTT CCCGTGCGAC TTTGGCCCTG GCGAGGTGCG CTACTCTCAC 
CTGGGCGCGC GCAGCCCGTC TCGGGACCGC GTCCGGCTGC AGCTGCGCTA TGACGCGCCC 
GGAGGGGCAG TAGTGCTACC ACTGGTACTG GAGGTGGAGG TGGTCTTCAC CCAGCTGGAG 
GTTGTGACTC GGAACTTGCC TCTGGTCGTG GAAGAGCTGC TGGGGACCAG CAATGCCCTG 
GACGCGCGGA GCCTGGAGTT CGCCTTCCAG CCCGAGACAG AGGAGTGCCG CGTGGGCATC 
CTGTCCGGCT TGGGCGCGCT GCCTCGCTAT GGAGAACTCC TCCACTACCC GCAGGTCCCT 
GGAGGAGCCA GAGAGGGAGG CGCCCCGGAG ACTCTCCTGA TGGACTGCAA AGCTTTCCAG 
GAACTAGGCG TGCGCTATCG CCACACAGCC GCCAGTCGCT CACCAAACAG GGACTGGATA 
CCCATGGTGG TGGAGCTGCG TTCACGAGGG GCTCCTGTGG GCAGCCCTGC TTTGAAACGC 
GAGCACTTCC AGGTTCTGGT GAGGATCCGA GGAGGGGCCG AGAACACTGC ACCCAAGCCC 
AGTTTCGTGG CCATGATGAT GATGGAGGTG GACCAGTTTG TACTGACGGC CCTGACCCCA 
GACATGCTGG CAGCCGAGGA TGCTGAGTCT CCCTCTGACC TGTTGATCTT CAACCTTACT 
TCTCCATTCC AGCCTGGCCA GGGCTACTTG GTGAGCACCG ATGATCGCAG CCTGCCCCTT 
TCCTCCTTCA CTCAGAGGGA TCTGCGGCTC CTGAAGATTG CCTACCAGCC CCCTTCTGAA 
GACTCTGACC AGGAGCGCCT CTTTGAACTG GAATTGGAGG TAGTGGATCT AGAAGGAGCA 
GCTTCAGACC CTTTTGCCTT CATGGTAGTG GTGAAGCCCA TGAACACAAT GGCTCCGGTG 
GTCACCCGGA ATACCGGTCT TATTCTCTAT GAGGGTCAGT CTCGGCCCCT CACAGGCCCT 
GCAGGCAGTG GTCCGCAAAA CTTGGTCATC AGCGATGAGG ATGACCTAGA AGCAGTGCGG 
CTAGAGGTGG TGGCTGGGCT CCGGCATGGT CACCTTGTCA TTCTGGGTGC TTCCAGTGGC 
AGCTCTGCTC CCAAGAGCTT TACAGTGGCT GAGCTGGCAG CCGGCCAGGT GGTCTACCAG 
CATGATGACA GAGACGGCTC GCTGAGCGAC AACCTGGTGC TTCGCATGGT GGATGGAGGA 
GGCAGGCACC AGGTACAGTT TCTGTTCCCC ATCACCTTAG TGCCTGTGGA TGACCAGCCA 
CCTGTTCTCA ATGCCAACAC GGGGCTGACA CTGGCAGAGG GTGAAACAGT GCCCATCCTG 
CCCCTTTCCC TGAGTGCAAC TGACATGGAT TCAGATGATT CTCTGCTGCT TTTTGTGCTG 
GAGTCACCCT TCTTAACTAC GGGGCATCTG CTTCTCCGCC AAACTCACCC TCCCCATGAG 
AAGCAGGAAC TTCTCAGAGG CCTTTGGAGG AAGGAGGGGG CATTTTATGA GCGAACAGTG 
ACAGAGTGGC AGCAGCAGGA CATAACAGAG GGCAGGCTGT TCTATAGACA CTCTGGGCCC 
CATAGTCCTG GGCCAGTCAC AGACCAGTTC ACATTTAGAG TCCAGGATAA CCATGACCCT 
CCTAATCAGT CCGGGCTACA GCGGTTTGTG ATTCGTATCC ATCCTGTGGA TCGCCTCCCT 
CCGGAGCTGG GCAGTGGCTG TCCCCTTCGT ATGGTGGTAC AGGAATCCCA GCTCACACCA 
CTGAGGAAGA AGTGGCTGCG CTACACTGAC CTGGACACAG ATGACCGAGA ACTACGTTAC 
ACAGTGACTC AGCCCCCCAC AGACACAGAC GAAAATCACC TGCCAGCCCC ACTGGGTACC 
TTGGTCTTGA CTGACAACCC CTCAGTCGTG GTGACCCATT TTACCCAAGC CCAGATCAAC 
CATCATAAAA TTGCTTACAG ACCCCCGGGT CAAGAACTGG GCGTGGCTAC TCGAGTGGCC 
CAGTTCCAGT TCCAGGTGGA AGACCGAGCT GGGAATGTGG CTCCAGGTAC CTTTACCCTT 
TACTTGCATC CCGTGGACAA CCAGCCACCT GAGATCCTCA ACACCGGCTT CACTATTCAG 
GAGAAGGGTC ACCACATCCT GAGTGAGACA GAGTTGCACG TGAATGATGT AGACACTGAT 
GTTGCCCATA TCTCTTTCAC TCTCACTCAG GCACCCAAAC ATGGCCACAT GAGAGTGTCT 
GGACAGATCC TGCATGTAGG GGGTCTCTTC CACTTGGAGG ACATAAAACA GGGCCGAGTT 
TCCTATGCCC ATAATGGGGA CAAGTCCCTG ACTGATAGCT GCTCCTTGGA AGTCAGTGAC 
AGACATCATG TGGTGCCCAT CACTCTCAGA GTAAATGTCC GGCCAGTGGA TGATGAAGTG 
CCCATACTGA GCCATCCTAC TGGCACTCTG GAGTCCTATC TAGATGTCTT AGAAAATGGG 
GCTACTGAAA TCACTGCCAA TGTTATTAAG GGGACCAATG AGGAAACTGA TGACTTGATG 
TTGACTTTCC TCTTGGAAGA TCCACCTTTG TATGGGGAAA TCTTGGTCAA TGGCATTCCA 
GCAGAGCAGT TTACTCAAAG GGACATCTTG GAGGGCTCTG TTGTATATAC CCACACCAGT 
GGTGAGATAG GCCTATTGCC TAAAGCGGAT TCTTTTAACC TGAGTCTGTC AGATATGTCT 
CAAGAATGGA GAATTGGTGG CAATACTATC CAAGGAGTTA CTATATGGGT GACCATCCTG 
CCTGTTGATA GCCAGGCCCC AGAAATCTTT GTAGGTGAAC AGTTGATAGT AATGGAAGGT 
GATAAAAGTG TTATAACATC AGTGCATATA AGTGCTGAAG ATGTCGACTC CCTGAATGAT 
GACATCTTGT GCACTATAGT TATTCAGCCT ACTTCAGGTT ATGTTGAAAA CATTTCTCCA 
GCACCAGGCT CTGAGAAATC AAGAGCAGGG ATTGCCATAA GTGCTTTCAA CTTGAAAGAT 
CTCAGGCAGG GCCACATAAA CTATGTCCAG AGTGTCCATA AAGGGGTGGA ACCTGTGGAG 
GACCGATTTG TATTTCGTTG TTCTGATGGC ATTAACTTTT CAGAGAGACA GTTCTTCCCC 
ATTGTAATCA TTCCCACCAA TGATGAACAG CCAGAGATGT TTATGAGAGA ATTTATGGTG 
ATGGAAGGCA TGAGTCTGGT AATTGATACA CCCATTCTCA ATGCTGCTGA TGCTGATGTT 
CCCCTGGATG ATTTAACTTT CACTATTACC CAATTCCCCA CTCATGGTCA CATCATGAAT 
CAGCTGATAA ATGGCACGGT TTTGGTCGAA AGCTTCACCT TGGATCAGAT CATAGAGAGT 
TCCAGCATTA TTTATGAGCA TGATGACTCC GAGACCCAGG AAGACAGTTT TGTGATTAAA 
CTAACAGATG GGAAGCACTC TGTGGAAAAG ACGGTCCTCA TTATAGTTAT CCCTGTTGAT 
GATGAGACGC CCAGAATGAC TATCAATAAT GGACTAGAAA TAGAAATTGG GGATACCAAG 
ATTATCAACA ACAAAATATT AATGGCAACA GATTTAGATT CAGAAGACAA ATCTTTGGTT 
TATATTATTC GTTATGGGCC AGGACATGGC TTATTACAGA GACGAAAACC TACTGGTGCC 
TTTGAAAATA TCACACTGGG CATGAATTTT ACCCAGGATG AAGTAGACAG AAACTTAATT 
CAGTATGTCC ATTTGGGGCA AGAGGGCATT CGGGACCTAA TTAAATTTGA TGTGACTGAT 
GGAATAAATC CCCTCATAGA TCGTTACTTT TATGTGTCCA TCGGGAGCAT TGACATTGTC 
TTCCCTGATG TGATAAGTAA GGGAGTGTCC TTGAAAGAAG GTGGCAAAGT CACTCTTACA 
ACAGACCTAC TAAGCACTAG TGACTTGAAC AGTCCTGATG AAAACTTGGT TTTTACCATC 
ACCAGGGCTC CCATGCGAGG TCACCTGGAA TGCACGGATC AGCCTGGTGT GTCCATCACG 
TCTTTCACTC AGCTGCAACT GGCTGGAAAC AAAATCTACT ACATCCACAC AGCTGATGAT 
GAAGTGAAAA TGGACAGTTT TGAGTTTCAA GTCACCGATG GACGTAACCC TGTCTTTCGG 
ACATTCCGTA TCTCCATTAG CGATGTGGAC AATAAAAAGC CAGTGGTCAC CATCCACAAG 
CTGGTTGTCA GTGAAAGTGA AAACAAGCTG ATTACTCCTT TTGAGCTCAC TGTCGAAGAC 
AGAGATACTC CTGACAAGCT CCTGAAATTC ACTATCACCC AGGTGCCTAT TCATGGCCAT 
CTCCTATTCA ACAATACCAG ACCTGTCATG GTTTTTACCA AGCAAGACTT GAATGAAAAC 
TTAATCAGCT ACAAACATGA TGGCACTGAG TCAAGTGAAG ATAGCTTCTC CTTCACAGTG 
ACTGATGGCA CCCATACAGA CTTCTATGTT TTTCCTGATA CGGTGTTTGA AACAAGGAGA 
CCCCAAGTGA TGAAGATCCA GGTCTTGGCT GTTGACAACA GTGTCCCCCA AATCGCAGTG 
AATAAGGGGG CCTCTACACT TCGCACTCTA GCCACTGGCC ACTTGGGGTT CATGATCACA 
AGCAAAATAT TGAAAGTGGA GGACAGAGAC AGCTTACACA TTTCTCTTAG ATTTATCGTG 
ACAGAGGCCC CTCAACATGG ATATCTTCTC AACCTGGACA AAGGCAACCA CAGCATCACT 
CAGTTCACAC AAGCTGACAT TGATGACATG AAAATATGCT ATGTCTTAAG AGAAGGGGCT 
AATGCCACAA GTGATATGTT CTATTTTGCA GTTGAAGATG GTGGTGGAAA CAAGTTAACG 
TACCAGAATT TTCGTCTGAA TTGGGCATGG ATCTCCTTTG AAAAGGAATA TTACCTGGTC 
AATGAGGACT CCAAATTTCT AGATGTTGTT CTTAAACGTA GAGGTTACTT GGGAGAAACT 
TCTTTTATAA GTATTGGCAC AAGAGACAGA ACTGCAGAAA AAGACAAAGA CTTCAAGGGC 
AAAGCACAGA AACAAGTGCA GTTCAACCCA GGCCAGACCA GGGCCACATG GCGAGTGCGG 
ATCCTGAGTG ATGGGGAGCA TGAGCAGTCT GAAACCTTTC AGGTGGTACT CTCAGAGCCC 
GTGCTGGCTG CCTTGGAATT CCCCACAGTC GCCACTGTTG AGATCGTTGA TCCAGGAGAT 
GAGCCAACTG TGTTTATTCC CCAGTCCAAA TACTCCGTTG AAGAAGATGT TGGTGAGCTG 
TTCATTCCCA TCAGGAGGAG CGGAGATGTG AGCCAGGAGT TGATGGTGGT CTGTTATACC 
CAACAAGGAA CAGCAACTGG AACTGTGCCG ACTTCCGTGT TGTCTTACTC TGATTACATA 
TCCAGGCCTG AGGACCACAC CAGTGTTGTC CGCTTTGACA AAGATGAACG GGAGAAACTG 
TGTCGGATAG TCATAATTGA TGACTCTTTG TACGAGGAGG AGGAAACCTT CCATGTCCTT 
CTGAGCATGC CCATGGGGGG AAGAATCGGA TCAGAGTTCC CAGGGGCTCA AGTTACAATC 
GTTCCTGACA AAGATGATGA ACCCATCTTT TACTTCGGTG ATGTGGAATA CTCTGTGGAT 
GAGAGTGCTG GCTATGTGGA AGTGCAGGTG TGGAGAACGG GCACTGACCT GTCCAAGTCT 
TCTAGTGTCA CAGTGAGGTC TCGGAAAACA GATCCTCCCT CTGCAGATGC TGGAACAGAC 
TATGTGGGCA TCAGCCGTAA TTTAGATTTT GCACCTGGAG TCAACATGCA GCCTGTTCGT 
GTTGTCATTC TGGATGACCT TGGACAACCA GCGCTGGAGG GAATTGAGAA ATTTGAACTG 
GTGCTTCGCA TGCCTATGAA CGCAGCCCTT GGCGAGCCCA GCAAAGCCAC AGTGTCCATA 
AATGACTCTG TCTCCGATTT GCCTAAGATG CAATTCAAAG AACGAATATA TACTGGCAGC 
GAAAGTGATG GGCAGATAGT TACAATGATC CATAGGACTG GGGATGTCCA GTACAGATCT 
TCAGTGAGAT GCTACACCCG GCAGGGGTCT GCACAGGTGA TGATGGACTT TGAAGAACGC 
CCAAACACTG ATACCTCCAT CATCACATTC CTCCCTGGTG AGACAGAAAA GCCCTGCATT 
CTTGAGCTGA TGGACGATGT GCTCTATGAG GAGGTAGAGG AGCTCCGCCT GGTACTCGGC 
ACTCCACAAA GCAACTCTCC CTTTGGGGCT GCAGTTGGTG AACAAAATGA AACTCTCATA 
AGGATCCGAG ATGATGCTGA TAAGACTGTT ATTAAATTTG GAGAAACCAA ATTTAGTGTC 
ACTGAACCCA AAGAACCTGG AGAGTCGGTG GTTATAAGAA TTCCAGTGAT TCGCCAAGGA 
GACACTTCAA AGGTTTCCAT TGTGAGAGTC CACACCAAGG ATGGCTCGGC CACCTCTGGA 
GAAGACTACC ACCCTGTGTC AGAAGAAATT GAGTTTAAGG AAGGGGAAAC CCAGCACGTG 
GTTGAAATCG AAGTTACCTT TGACGGGGTG AGAGAGATGA GAGAGGCCTT CACTGTTCAC 
CTAAAACCTG ATGAAAATAT GATAGCAGAG ATGCAGTTGA CGAAAGCCAT TGTGTACATA 
GAAGAAATGA GCAGCATGGC AGATGTCACT TTTCCTTCTG TCCCTCAAAT TGTATCCCTG 
TTGATGTATG ACGACACTTC CAAAGCTAAG GAGAGTGCTG AACCCATGTC TGGCTATCCT 
GTCATCTGTA TCACAGCTTG CAACCCCAAA TATTCAGACT ACGATAAAAC AGGCTCTATC 
TGTGCAAGTG AGAACATCAA TGACACTTTG ACGCGGTACC GGTGGCTGAT TAGTGCACCT 
GCGGGCCCTG ACGGTGTGAC CAGCCCTATG AGAGAAGTGG ACTTCGACAC CTTTTTTACG 
TCATCCAAGA TGGTCACACT GGACTCCATA TACTTTCAGC CTGGCTCCCG GGTACAGTGC 
GCAGCTCGTG CTGTGAACAC CAATGGGGAT GAAGGCCTGG AGCTCATGAG CCCTATTGTA 
ACCATCAGCA GAGAAGAAGG TCTTTGTCAG CCCCGTGTAC CTGGGGTTGT TGGAGCAGAG 
CCGTTCTCAG CTAAATTGCG CTACACAGGC CCTGAGGATG CAGACTACAC AAACCTTATC 
AAGCTCACTG TCACAATGCC ACACATAGAT GGCATGCTCC CCGTGATCTC CACTAGAGAG 
CTTTCCAACT TTGAGCTCAC CCTCAGCCCT GATGGCACAA GAGTTGGAAA CCACAAGTGC 
TCCAACCTCC TGGATTATAC TGAAGTGAAG ACTCATTATG GTTTCTTGAC TGATGCTACC 
AAAAATCCAG AAATAATTGG AGAGACATAT CCTTACCAGT ACAGCTTGTC CATCAGAGGT 
TCCACTACCT TGCGCTTCTA CCGGAACCTG AACCTAGAGG CCTGTTTATG GGAGTTCGTT 
AGCTACTATG ACATGTCAGA ACTCCTTGCT GACTGTGGTG GCACCATTGG AACAGATGGA 
CAGGTCCTAA ACCTAGTGCA GTCCTATGTG ACCCTTCGAG TCCCTCTGTA TGTTTCCTAC 
GTGTTCCATT CCCCCGTGGG GGTAGGAGGC TGGCAGCATT TTGACTTGAA GTCAGAGCTT 
CGTCTAACTT TTGTGTACGA CACCGCCATC TTGTGGAATG ATGGAATTGG CAGCCCCCCA 
GAGGCTGAAC TTCAAGGTTC TCTCTATCCA ACCAGCATGC GCATCGGTGA TGAGGGGCGC 
TTGGCCGTGC ACTTCAAGAC AGAGGCTCAG TTCCATGGCT TATTTGTGCT GTCACATCCC 
GCATCCTTTA CAAGCTCAGT GATCATGTCA GCTGATCATC CAGGCCTGAC ATTTTCCCTC 
CGCCTCATAA GGAGTGAACC AACCTATAAC CAGCCAGTAC AGCAGTGGAG CTTTGTCTCT 
GACTTTGCCG TACGTGACTA CTCAGGGACC TATACTGTGA AGCTGGTGCC ATGCACTGCC 
CCATCACATC AGGAATACCG CCTGCCAGTC ACCTGCAACC CCAGAGAACC TGTCACCTTT 
GACCTTGACA TCCGATTCCA ACAGGTCAGT GATCCAGTGG CTGCTGAGTT TAGCTTGAAC 
ACCCAAATGT ACCTGCTCTC TAAGAAGAGT CTCTGGTTGT CTGATGGATC CATGGGATTC 
GGGCAAGAGA GTGATGTTGC TTTTGCAGAA GGTGATATAA TTTATGGTCG TGTCATGGTG 
GATCCTGTCC AGAATCTGGG TGACTCCTTT TACTGCAGCA TTGAGAAGGT GTTTCTATGC 
ACTGGAGCTG ATGGCTATGT TCCCAAGTAT AGTCCAATGA ATGCAGAATA TGGCTGCTTA 
GCCGACTCTC CTTCACTCTT ATATAGATTT AAAATTGTGG ACAAAGCTCA GCCAGAGACA 
CAAGCGACCA GTTTTGGAAA TGTCCTATTT AATGCCAAAC TAGCAGTGGA TGACCCTGAA 
GCCATTCTCT TAGTGAATCA GCCTGGATCT GATGGATTTA AAGTCGACTC AACACCACTC 
TTTCAGGTCG CTCTAGGCCG AGAATGGTAT ATACATACGA TCTATACAGT GAGATCGAAA 
GACAATGCCA ATCGAGGTAT TGGCAAAAGA AGTGTGGAGT ACCATTCTCT GGTGAGTCAA 
GGAAAGCCCC AATCCACCAC CAAGAGCCGG AAGAAGAGAG AGATCAGGAG CACACCCTCA 
CTGGCATGGG AGATTGGTGC TGAAAACAGT CGAGGAACAA ACATCCAGCA CATTGCCCTG 
GACCGCACCA AGAGGCAGAT CCCCCATGGG AGAGCACCTC CAGATGGCAT CCTCCCCTGG 
GAGCTCAACA GCCCCAGCTC TGCAGTCAGC CTGGTCACTG TGGTGGGAGG CACCACGGTA 
GGGTTACTCA CCATCTGCCT CACTGTCATT GCAGTGCTGA TGTGCAGGGG CAAGGAAAGT 
TTCAGGGGGA AGGATGCCCC GAAAGGCTCC AGCAGCAGTG AGCCCATGGT GCCCCCACAG 
AGCCATCACA ATGACAGCTC AGAAGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mild recessive mutations in six Fraser syndrome-related genes cause isolated congenital anomalies of the kidney and urinary tract.
Journal of the American Society of Nephrology : JASN25(9)1917-22(2014 Sep)
Kohl S,Hwang DY,Dworschak GC,Hilger AC,Saisawat P,Vivante A,Stajic N,Bogdanovic R,Reutter HM,Kehinde EO,Tasic V,Hildebrandt F

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FREM2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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