OTOG cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	OTOG
Entrez Gene ID	340990
Full Name	otogelin
Synonyms	DFNB18B,MLEMP,OTGN
General protein information	Preferred Names otogelin Names otogelin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p15.1
Summary	The protein encoded by this gene is a component of the acellular membranes of the inner ear. Disruption of the orthologous mouse gene shows that it plays a role in auditory and vestibular functions. It is involved in fibrillar network organization, the anchoring of otoconial membranes and cupulae to the neuroepithelia, and likely in sound stimulation resistance. Mutations in this gene cause autosomal recessive nonsyndromic deafness, type 18B. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, May 2014].
Disorder MIM:	604487
Disorder Html:	Deafness, autosomal recessive 18B, 614945 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001292063.1	NP_001278992.1	otogelin isoform b precursor
NM_001277269.2	NP_001264198.1	otogelin isoform a precursor
NM_001277269.1	NP_001264198.1	otogelin isoform a precursor
NM_001292063.2	NP_001278992.1	otogelin isoform b precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	OTOG	NP_001264198.1
Pan troglodytes (chimpanzee)	OTOG	XP_521850.3
Canis lupus familiaris (dog)	OTOG	XP_005633867.1
Gallus gallus (chicken)	OTOG	XP_421007.4
Rattus norvegicus (Norway rat)	Otog	NP_001099732.1
	OTOG	XP_002799664.1
Mus musculus (house mouse)	Otog	NP_038652.2
Bos taurus (cattle)	OTOG	XP_002693137.2

Gene Ontology
Bibliography

Related articles in PubMed

Otolith tethering in the zebrafish otic vesicle requires Otogelin and ?-Tectorin.
Stooke-Vaughan GA, Obholzer ND, Baxendale S, Megason SG, Whitfield TT
Development (Cambridge, England)142(6)1137-45(2015 Mar)

Similar phenotypes caused by mutations in OTOG and OTOGL.
Oonk AM, Leijendeckers JM, Huygen PL, Schraders M, del Campo M, del Castillo I, Tekin M, Feenstra I, Beynon AJ, Kunst HP, Snik AF, Kremer H, Admiraal RJ, Pennings RJ
Ear and hearing35(3)e84-91(2014 May-Jun)

A phylomedicine approach to understanding the evolution of auditory sensory perception and disease in mammals.
Kirwan JD, Bekaert M, Commins JM, Davies KT, Rossiter SJ, Teeling EC
Evolutionary applications6(3)412-22(2013 Apr)

Mutations of the gene encoding otogelin are a cause of autosomal-recessive nonsyndromic moderate hearing impairment.
Schraders M, Ruiz-Palmero L, Kalay E, Oostrik J, del Castillo FJ, Sezgin O, Beynon AJ, Strom TM, Pennings RJ, Zazo Seco C, Oonk AM, Kunst HP, Dom?nguez-Ruiz M, Garc?a-Arumi AM, del Campo M, Villamar M, Hoefsloot LH, Moreno F, Admiraal RJ, del Castillo I, Kremer H
American journal of human genetics91(5)883-9(2012 11)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Patients with OTOG mutation show a flat to downsloping configuration of the audiogram with mild to moderate sensorineural hearing loss. Speech recognition scores remain good and vestibular hyporeflexia is present.
Title: Similar phenotypes caused by mutations in OTOG and OTOGL.

study reports the identification of mutations in OTOG as a cause of moderate nonsyndromic hearing loss
Title: Mutations of the gene encoding otogelin are a cause of autosomal-recessive nonsyndromic moderate hearing impairment.

The following OTOG gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the OTOG cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25668	NM_001292063.2 Latest version!	Homo sapiens otogelin (OTOG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu27814	NM_001277269.2 Latest version!	Homo sapiens otogelin (OTOG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
OHu27814	NM_001277269.1	Homo sapiens otogelin (OTOG), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
OHu25668	NM_001292063.1	Homo sapiens otogelin (OTOG), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

OTOG ( NM_001292063.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278992.1 Homo sapiens otogelin (OTOG), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25668
Clone ID Related Accession (Same CDS sequence)	NM_001292063.1 , NM_001292063.2
Accession Version	NM_001292063.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-11
Organism	Homo sapiens(human)
Product	otogelin isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124799.5, EG328175.1, EG328176.1, DV080384.1, DB081210.1, DV080242.1, DV080216.1, DV080434.1, DB096404.1, DB341780.1 and DV080398.1. Transcript Variant: This variant (2) uses two alternate splice sites and includes an additional exon, which together result in an alternate in-frame segment in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The exon combination of this transcript is inferred from the full-length mouse mRNA U96411.1, and is supported by partial human transcript alignments, RNA-seq data, and partial RT-PCR data from PMID:23122587. CCDS Note: This CCDS representation lacks full-length human transcript support. Its exon combination is therefore inferred, but it is supported by the full-length orthologous mouse transcript U96411.1, by partial human transcript alignments, and by data in PMID:23122587. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGCCGCAG CACCCGTTCT GTGGGGCAGT 
GCAGAGCCAC AGCCAGAGCC AGCCGGGCAA CCCAGCAGCA GCCACCAGGA GGCGACCCTT 
GCCATGGGGG ACAAGGCTAC AGTCGTGGGA GGCCAGCAGG CTGAAGCCCC AGACTCCGTG 
GCCATGTCTT CCTGGGAAAG GCGGCTCCAT CGGGCCAAGT GTGCACCATC CTACTTGTTC 
TCCTGCTTCA ATGGAGGCGA GTGTGTGCAC CCAGCCTTCT GTGACTGCAG ACGCTTCAAT 
GCCACTGGAC CGCGCTGCCA GATGGTGTAC AATGCCGGCC CTGAGAGGGA CAGCATTTGC 
CGGGCGTGGG GGCAGCACCA CGTGGAGACA TTTGATGGGC TCTACTACTA CCTCTCCGGA 
AAGGGCAGCT ACACCCTGGT GGGTCGCCAT GAGCCCGAGG GACAGAGCTT CTCCATCCAG 
GTACACAATG ACCCGCAGTG TGGCTCTTCA CCCTACACCT GCTCCAGGGC TGTCAGCCTC 
TTCTTTGTGG GTGAGCAGGA GATCCATCTG GCCAAGGAGG TCACCCATGG AGGCATGAGG 
GTCCAACTGC CACATGTCAT GGGGAGCGCG CGTCTGCAGC AGCTTGCCGG CTATGTCATC 
GTGCGGCATC AGTCAGCCTT CACACTGGCC TGGGATGGTG CCTCGGCTGT CTACATCAAG 
ATGAGTCCAG AGCTTCTGGG CTGGACCCAT GGGCTGTGTG GGAACAACAA TGCTGACCCC 
AAGGATGATC TGGTGACCAG CTCTGGGAAG CTGACTGACG ACGTGGTTGA GTTTGTGCAC 
AGCTGGCAGG AGCAGGCCCC TAACCAGCCT CCAGGGCCCA CAACTTCCTC CCTGCCTCGC 
CCACCGTGCC TACAGCAGAA CCCAGGAACC ATGCAGGGCG TGTACGAGCA GTGTGAGGCT 
CTACTGCGGC CCCCCTTTGA CGCCTGCCAC GCCTACGTCA GCCCTCTGCC CTTCACAGCC 
AGTTGTACCA GTGATCTCTG CCAATCAATG GGTGATGTAG CCACCTGGTG CCGGGCACTG 
GCGGAGTATG CCCGGGCGTG TGCCCAGGCA GGGCGGCCCT TGCAAGGCTG GAGGACCCAG 
CTCCGGCAAT GCACTGTGCA CTGCAAGGAG AAGGCCTTTA CCTACAATGA GTGCATCGCC 
TGCTGCCCTG CCTCCTGCCA TCCCCGGGCA TCCTGTGTGG ACAGTGAGAT CGCCTGTGTG 
GACGGCTGCT ATTGCCCCAA TGGGCTCATC TTCGAGGATG GGGGCTGCGT GGCACCAGCT 
GAGTGTCCCT GTGAGTTTCA CGGGACTCTG TACCCACCTG GCTCTGTGGT GAAGGAAGAC 
TGCAATACTT GCACATGCAC CTCAGGCAAG TGGGAGTGCA GCACAGCTGT CTGCCCAGCT 
GAGTGCTCAG TGACTGGTGA CATTCACTTC ACAACCTTTG ATGGCCGCCG GTACACGTTC 
CCCGCCACAT GTCAGTACAT CCTGGCCAAG AGCCGCTCTT CGGGCACCTT CACCGTGACA 
TTGCAGAATG CCCCATGTGG CCTGAACCAA GATGGAGCCT GTGTCCAGTC AGTGTCAGTG 
ATTCTGCACC AGGACCCTCG GAGGCAGGTG ACCCTGACCC AGGCAGGGGA TGTCCTTCTG 
TTTGACCAGT ACAAGATCAT CCCGCCATAC ACAGATGATG CCTTTGAGAT CCGTAGGCTG 
TCCTCCGTGT TCCTGCGGGT GAGGACGAAC GTGGGCGTGC GGGTGCTCTA CGACCGTGAA 
GGGCTCCGAC TGTACCTGCA AGTGGACCAG CGATGGGTGG AGGATACCGT GGGCCTCTGC 
GGCACCTTCA ATGGCAACAC GCAGGATGAC TTCCTGTCTC CAGTGGGTGT ACCTGAGAGC 
ACCCCACAAC TTTTTGGCAA TTCCTGGAAA ACACTTTCTG CTTGCTCCCC GCTGGTCTCT 
GGCTCCCCTC TGGACCCCTG CGATGTGCAC CTGCAAGCCG CCTCCTACTC AGTGCAGGCC 
TGCAGCGTGC TCACGGGGGA GATGTTTGCG CCCTGCTCTG CGTTCCTGAG CCCCGTGCCC 
TACTTTGAGC AGTGCCGCAG GGATGCCTGC CGCTGCGGGC AGCCCTGCCT GTGCGCCACA 
CTGGCCCACT ACGCCCACCT GTGCCGGCGC CATGGGCTCC CCGTTGATTT CCGCGCCCGC 
CTGCCAGCCT GTGCACTGTC CTGTGAGGCC TCCAAGGAGT ATAGCCCCTG CGTGGCCCCG 
TGTGGACGTA CCTGCCAGGA CCTGGCCAGC CCTGAGGCCT GTGGGGTTGA TGGTGGCGAT 
GACCTGAGCA GAGACGAGTG TGTGGAGGGC TGTGCCTGCC CACCGGACAC CTATCTGGAC 
ACCCAGGCTG ACCTCTGTGT CCCCCGGAAC CAGTGCTCCT GCCACTTCCA GGGAGTGGAC 
TATCCCCCCG GAGACAGTGA CATCCCATCC CTGGGCCACT GCCACTGCAA AGATGGAGTC 
ATGAGCTGTG ATAGCAGAGC CCCAGCTGCT GCCTGCCCAG CAGGCCAGGT CTTCGTGAAC 
TGCAGCGACC TGCACACGGA CCTGGAGCTG AGCAGGGAGA GGACGTGTGA GCAGCAACTG 
CTGAACCTGA GCGTGTCAGC CCGTGGCCCC TGCCTCTCGG GCTGCGCCTG TCCCCAGGGT 
CTGCTCAGAC ACGGGGATGC ATGTTTCCTG CCAGAGGAGT GCCCCTGCAC TTGGAAGGGG 
AAGGAGTATT TCCCTGGGGA CCAGGTGATG TCTCCTTGCC ATACCTGTGT GTGCCAGCGG 
GGCTCATTCC AGTGCACCCT GCACCCTTGC GCCTCCACCT GCACTGCCTA TGGGGACCGG 
CATTACCGCA CGTTTGATGG GCTCCCGTTT GACTTCGTGG GGGCATGCAA AGTGCACCTG 
GTCAAGAGCA CATCAGATGT CAGCTTCTCT GTGATTGTAG AGAATGTGAA CTGCTACAGC 
TCTGGCATGA TCTGCAGGAA ATTTATTTCC ATCAACGTTG GGAACTCACT CATTGTCTTT 
GATGATGACT CCGGAAATCC TAGTCCAGAG AGCTTCCTGG ATGACAAGCA GGAGGTCCAC 
ACATGGCGAG TGGGATTTTT CACACTGGTG CATTTCCCAC AGGAGCACAT CACCCTCTTG 
TGGGACCAGA GAACCACAGT GCACGTCCAG GCTGGGCCTC AGTGGCAGGG CCAGCTGGCG 
GGCCTCTGTG GGAACTTTGA CTTAAAAACC ATCAATGAGA TGAGGACCCC GGAGAACCTA 
GAGCTAACTA ACCCCCAGGA GTTTGGCAGC AGTTGGGCTG CAGTTGAGTG CCCAGACACC 
CTCGATCCTC GGGATATGTG TGTCCTGAAT CCTCTCCGAG AACCATTTGC CAAGAAGGAG 
TGCAGCATCC TGCTCAGTGA GGTGTTTGAG ATCTGCCACC CTGTGGTTGA TGTCACTTGG 
TTTTACTCAA ACTGCCTGAC AGACACATGT GGCTGCAGCC AGGGTGGTGA CTGTGAGTGC 
TTCTGTGCCA GCGTCTCCGC TTATGCCCAC CAGTGTTGCC AGCATGGGGT GGCTGTTGAC 
TGGCGAACCC CCCGCCTCTG CCCGTATGAC TGTGACTTCT TTAACAAAGT GCTAGGTAAG 
GGCCCCTATC AGCTATCCAG CTTGGCAGCC GGTGGTGCTC TGGTGGGCAT GAAGGCGGTG 
GGCGATGACA TAGTCCTAGT GAGGACAGAG GATGTGGCGC CAGCAGACAT TGTGAGCTTC 
CTGCTGACAG CTGCTCTGTA CAAGGCCAAG GCCCATGACC CAGATGTGGT GTCCCTGGAG 
GCAGCAGACA GACCCAACTT CTTCCTTCAC GTCACAGCCA ACGGGTCTCT GGAGCTGGCT 
AAGTGGCAGG GCCGTGACAC CTTCCAACAG CATGCCTCCT TCTTGCTGCA CCGGGGGACA 
CGGCAGGCAG GCCTGGTGGC CCTGGAGTCC CTGGCCAAGC CCAGCTCCTT CCTCTATGTG 
TCGGGCGCGG TGCTGGCCCT GCGGCTGTAC GAACACACAG AGGTGTTCCG CCGGGGCACA 
CTCTTCCGCC TTCTGGATGC CAAGCCCTCG GGGGCTGCCT ACCCCATCTG CGAGTGGCGC 
TACGATGCCT GTGCCAGCCC CTGCTTCCAA ACCTGCCGGG ACCCACGGGC AGCCAGCTGC 
CGGGACGTAC CCAGGGTAGA AGGCTGTGTC CCTGTGTGCC CCACCCCCCA GGTCCTGGAT 
GAAGTCACAC AGAGATGTGT CTACTTGGAG GACTGTGTGG AGCCAGCAGT TTGGGTTCCC 
ACAGAGGCCC TTGGCAATGA GACCCTCCCT CCCAGTCAAG GGTTGCCCAC TCCCAGTGAT 
GAGGAGCCAC AGCTGTCACA GGAAAGCCCC AGGACCCCCA CCCACAGGCC AGCCCTCACC 
CCAGCTGCCC CACTCACCAC AGCCCTGAAC CCACCAGTGA CAGCCACTGA GGAGCCAGTG 
GTGTCTCCAG GCCCCACCCA GACCACCCTG CAGCAGCCAC TGGAGCTCAC TGCATCTCAA 
CTCCCCGCCG GCCCCACGGA GTCCCCAGCC AGCAAGGGAG TGACTGCCAG CCTCCTGGCC 
ATCCCCCATA CACCAGAGTC CTCATCCCTC CCTGTTGCAC TGCAGACACC CACACCTGGC 
ATGGTGTCAG GTGCCATGGA GACAACAAGG GTGACTGTGA TCTTTGCAGG AAGCCCTAAC 
ATCACAGTCT CCTCCCGGTC GCCCCCTGCC CCTCGCTTCC CGCTCATGAC CAAGGCTGTG 
ACAGTCCGAG GCCATGGCTC CTTGCCTGTT AGGACGACAC CCCCACAGCC CTCCTTGACA 
GCAAGTCCCT CCTCCAGACC TGTGGCTTCC CCTGGAGCCA TCTCCAGGTC CCCCACCTCC 
TCGGGATCCC ACAAGGCTGT GCTGACACCT GCAGTAACTA AGGTCATAAG CAGGACAGGG 
GTCCCCCAGC CCACCCAGGC CCAGAGTGCT TCAAGTCCCA GCACCCCTCT AACTGTGGCT 
GGAACAGCAG CAGAACAGGT TCCTGTCAGT CCCCTTGCAA CCAGGAGCTT GGAGATAGTG 
CTATCCACAG AGAAGGGCGA AGCCGGGCAC AGCCAGCCCA TGGGCTCGCC TGCCTCCCCA 
CAGCCACACC CACTCCCCTC TGCACCACCC CGCCCAGCCC AGCATACCAC CATGGCCACC 
AGGTCTCCAG CTCTGCCCCC AGAGACCCCA GCTGCCGCCA GCCTGTCAAC AGCCACTGAT 
GGGCTGGCAG CCACACCCTT CATGTCCCTT GAGTCAACTC GTCCCTCCCA GCTCCTCTCT 
GGCCTGCCTC CCGACACCAG CCTGCCCCTG GCCAAGGTGG GCACATCTGC CCCAGTGGCC 
ACACCCGGCC CCAAAGCCTC TGTCATCACC ACTCCACTCC AGCCACAGGC CACGACTCTG 
CCTGCTCAGA CACTTAGCCC AGTACTGCCT TTCACTCCAG CAGCAATGAC CCAGGCGCAC 
CCACCCACTC ACATAGCACC CCCAGCAGCA GGCACAGCTC CAGGCCTGCT GCTGGGAGCC 
ACATTGCCAA CCTCTGGAGT CCTGCCTGTG GCTGAGGGCA CGGCCTCCAT GGTATCTGTT 
GTCCCACGAA AGAGCACCAC AGGGAAGGTG GCCATCCTAT CCAAGCAAGT GTCTCTGCCC 
ACTTCCATGT ATGGTTCTGC AGAGGGTGGG CCCACAGAGC TCACGCCTGC TACGAGCCAC 
CCTCTCACGC CCTTGGTGGC TGAGCCCGAG GGAGCCCAGG CAGGCACAGC TCTGCCAGTG 
CCCACATCCT ATGCCCTGAG CCGTGTCTCA GCCAGGACGG CCCCCCAAGA CAGCATGCTG 
GTTCTGTTGC CTCAGCTGGC TGAGGCCCAT GGAACCTCGG CAGGGCCTCA CCTGGCAGCA 
GAGCCGGTGG ACGAGGCCAC CACAGAACCA TCTGGGCGCT CAGCCCCAGC CCTGAGCATC 
GTAGAGGGTT TGGCGGAGGC TTTGGCAACT ACCACTGAGG CCAATACATC CACCACCTGT 
GTTCCAATCG CCGAGCAGGA CTGCGTCCGC CACATCTGCC TGGAGGGCCA GCTGATTCGC 
GTGAATCAGT CCCAGCACTG TCCCCAGGGT GCTGCTCCCC CTCGCTGTGG GATCCTGGGC 
CTCGCCGTGC GGGTGGGTGG GGACCGCTGC TGCCCACTCT GGGAGTGTGC CTGCCGGTGC 
TCAATCTTCC CTGACCTGAG CTTCGTGACC TTCGATGGGA GCCACGTAGC TCTGTTCAAG 
GAGGCCATCT ACATCCTCAG CCAGAGCCCA GATGAAATGC TCACCGTCCA TGTACTGGAC 
TGCAAAAGTG CCAACCTGGG GCACCTGAAC TGGCCCCCGT TCTGTCTGGT GATGTTGAAC 
ATGACTCACT TGGCCCATCA GGTCACTATT GATCGCTTCA ACCGAAAGGT GACTGTGGAC 
TTGCAGCCTG TGTGGCCACC GGTGAGCAGG TATGGATTCA GAATTGAGGA CACAGGCCAC 
ATGTACATGA TCCTGACTCC CTCAGACATC CAGATCCAGT GGCTCCACAG CTCAGGACTC 
ATGATCGTGG AGGCCAGCAA AACCAGCAAG GCCCAGGGCC ATGGCCTGTG CGGTATCTGT 
GATGGAGATG CAGCCAATGA CCTTACCCTG AAGGATGGCT CAGTGGTGGG TGGGGCTGAG 
GACCCTGCTC CCTTTCTGGA CAGCTGGCAG GTGCCCAGCT CCCTGACCTC AGTGGGCCAG 
ACCCGCTTCC GCCCAGACAG CTGCGCCACA ACTGACTGCT CGCCCTGCCT TCGCATGGTG 
TCCAACCGCA CCTTCAGTGC CTGCCACCGC TTTGTGCCTC CGGAGTCATT CTGTGAGCTG 
TGGATCCGGG ACACCAAGTA CGTGCAGCAG CCCTGCGTGG CCCTGACTGT GTACGTGGCC 
ATGTGCCACA AATTTCATGT GTGCATCGAG TGGCGGCGCT CTGACTACTG CCCCTTCCTG 
TGCTCCAGCG ACTCCACATA CCAGGCATGT GTGACAGCCT GTGAGCCACC CAAGACATGC 
CAGGATGGGA TACTAGGGCC TCTGGACCCA GAGCACTGCC AGGTGCTGGG CGAGGGCTGC 
GTCTGCTCCG AGGGCACCAT CTTACACCGG CGCCACTCTG CACTCTGCAT CCCGGAGGCC 
AAGTGCGCCT GCACTGACAG CATGGGGGTG CCGAGGGCCC TGGGGGAGAC CTGGAACAGC 
TCCCTCAGCG GCTGCTGCCA GCACCAGTGC CAAGCCCCAG ACACCATTGT CCCGGTGGAT 
CTGGGCTGCC CCAGTCCCCG CCCTGAGAGC TGCCTGCGAT TCGGGGAGGT GGCCTTGCTC 
CTACCCACCA AGGACCCCTG CTGCCTGGGG ACTGTCTGTG TGTGTAACCA GACTCTGTGT 
GAGGGTCTCG CCCCCACATG CCGCCCAGGC CACCGCCTCC TCACCCACTT CCAGGAGGAC 
TCCTGCTGCC CCAGCTACAG CTGTGAGTGT GACCCAGATC TCTGTGAGGC AGAGCTGGTC 
CCCAGCTGCC GACAGGACCA GATCCTGATC ACGGGCCGCC TGGGGGACTC CTGCTGCACC 
TCCTACTTCT GCGCCTGTGG TGACTGTCCA GACTCCATCC CCGAATGTCA AGAAGGGGAG 
GCGCTCACTG TGCACAGGAA TACCACGGAA CTCTGCTGCC CTCTGTACCA GTGTGTGTGT 
GAGAACTTCC GCTGTCCCCA AGTGCAGTGT GGCCTGGGCA CTGCCCTGGT GGAGGTGTGG 
AGCCCCGACC GCTGCTGCCC CTACAAATCC TGTGAATGTG ACTGTGACAC AATCCCGGTG 
CCCCGGTGCC ATCTGTGGGA GAAATCCCAG CTGGATGAGG AGTTCATGCA CAGCGTGGAG 
AATGTGTGTG GCTGCGCCAA GTACGAGTGT GTGAAGGCCC CGGTGTGTCT GAGCCGCGAG 
CTGGGTGTGA TGCAGCCCGG CCAGACAGTG GTGGAGCTCT CAGCAGATGG CGTGTGCCAC 
ACCTCCCGCT GCACCACCGT GCTCGACCCT CTCACCAACT TCTACCAGAT CAACACCACC 
TCCGTGCTCT GTGACATCCA CTGTGAGGCG AACCAGGAGT ACGAGCACCC GCGGGACCTC 
GCTGCCTGCT GCGGCTCCTG CAGGAACGTG TCCTGTCTCT TCACCTTCCC CAATGGCACC 
ACCTCCCTGT TCTTGCCCGG GGCATCCTGG ATCGCAGACT GCGCCCGCCA CCACTGCAGC 
AGCACGCCCC TGGGTGCCGT GCTGGTCCGC TCTCCCATAA GCTGCCCACC GCTCAATGAG 
ACTGAGTGTG CCAAGGTTGG GGGTTCCGTG GTACCTTCCT TGGAAGGATG CTGCAGGACC 
TGTAAGGAGG ATGGGCGCTC CTGCAAGAAG GTGACCATCC GCATGACCAT CCGCAAGAAT 
GAATGCAGGA GCAGCACCCC TGTGAACCTA GTGTCCTGCG ATGGGAGGTG CCCATCCGCC 
AGCATCTACA ACTACAACAT CAACACCTAT GCCCGATTCT GCAAGTGCTG CCGTGAGGTG 
GGCCTGCAGC GGCGCTCTGT GCAGCTCTTC TGTGCCACCA ATGCCACCTG GGTGCCCTAT 
ACAGTGCAGG AGCCCACCGA CTGTGCCTGC CAGTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278992.1
CDS	1..8742
Translation	MGVLASALCWLLCVWLPWGEQAAESLRVQRLAAAPVLWGSAEPQPEPAGQPSSSHQEATLAMGDKATVVGGQQAEAPDSVAMSSWERRLHRAKCAPSYLFSCFNGGECVHPAFCDCRRFNATGPRCQMVYNAGPERDSICRAWGQHHVETFDGLYYYLSGKGSYTLVGRHEPEGQSFSIQVHNDPQCGSSPYTCSRAVSLFFVGEQEIHLAKEVTHGGMRVQLPHVMGSARLQQLAGYVIVRHQSAFTLAWDGASAVYIKMSPELLGWTHGLCGNNNADPKDDLVTSSGKLTDDVVEFVHSWQEQAPNQPPGPTTSSLPRPPCLQQNPGTMQGVYEQCEALLRPPFDACHAYVSPLPFTASCTSDLCQSMGDVATWCRALAEYARACAQAGRPLQGWRTQLRQCTVHCKEKAFTYNECIACCPASCHPRASCVDSEIACVDGCYCPNGLIFEDGGCVAPAECPCEFHGTLYPPGSVVKEDCNTCTCTSGKWECSTAVCPAECSVTGDIHFTTFDGRRYTFPATCQYILAKSRSSGTFTVTLQNAPCGLNQDGACVQSVSVILHQDPRRQVTLTQAGDVLLFDQYKIIPPYTDDAFEIRRLSSVFLRVRTNVGVRVLYDREGLRLYLQVDQRWVEDTVGLCGTFNGNTQDDFLSPVGVPESTPQLFGNSWKTLSACSPLVSGSPLDPCDVHLQAASYSVQACSVLTGEMFAPCSAFLSPVPYFEQCRRDACRCGQPCLCATLAHYAHLCRRHGLPVDFRARLPACALSCEASKEYSPCVAPCGRTCQDLASPEACGVDGGDDLSRDECVEGCACPPDTYLDTQADLCVPRNQCSCHFQGVDYPPGDSDIPSLGHCHCKDGVMSCDSRAPAAACPAGQVFVNCSDLHTDLELSRERTCEQQLLNLSVSARGPCLSGCACPQGLLRHGDACFLPEECPCTWKGKEYFPGDQVMSPCHTCVCQRGSFQCTLHPCASTCTAYGDRHYRTFDGLPFDFVGACKVHLVKSTSDVSFSVIVENVNCYSSGMICRKFISINVGNSLIVFDDDSGNPSPESFLDDKQEVHTWRVGFFTLVHFPQEHITLLWDQRTTVHVQAGPQWQGQLAGLCGNFDLKTINEMRTPENLELTNPQEFGSSWAAVECPDTLDPRDMCVLNPLREPFAKKECSILLSEVFEICHPVVDVTWFYSNCLTDTCGCSQGGDCECFCASVSAYAHQCCQHGVAVDWRTPRLCPYDCDFFNKVLGKGPYQLSSLAAGGALVGMKAVGDDIVLVRTEDVAPADIVSFLLTAALYKAKAHDPDVVSLEAADRPNFFLHVTANGSLELAKWQGRDTFQQHASFLLHRGTRQAGLVALESLAKPSSFLYVSGAVLALRLYEHTEVFRRGTLFRLLDAKPSGAAYPICEWRYDACASPCFQTCRDPRAASCRDVPRVEGCVPVCPTPQVLDEVTQRCVYLEDCVEPAVWVPTEALGNETLPPSQGLPTPSDEEPQLSQESPRTPTHRPALTPAAPLTTALNPPVTATEEPVVSPGPTQTTLQQPLELTASQLPAGPTESPASKGVTASLLAIPHTPESSSLPVALQTPTPGMVSGAMETTRVTVIFAGSPNITVSSRSPPAPRFPLMTKAVTVRGHGSLPVRTTPPQPSLTASPSSRPVASPGAISRSPTSSGSHKAVLTPAVTKVISRTGVPQPTQAQSASSPSTPLTVAGTAAEQVPVSPLATRSLEIVLSTEKGEAGHSQPMGSPASPQPHPLPSAPPRPAQHTTMATRSPALPPETPAAASLSTATDGLAATPFMSLESTRPSQLLSGLPPDTSLPLAKVGTSAPVATPGPKASVITTPLQPQATTLPAQTLSPVLPFTPAAMTQAHPPTHIAPPAAGTAPGLLLGATLPTSGVLPVAEGTASMVSVVPRKSTTGKVAILSKQVSLPTSMYGSAEGGPTELTPATSHPLTPLVAEPEGAQAGTALPVPTSYALSRVSARTAPQDSMLVLLPQLAEAHGTSAGPHLAAEPVDEATTEPSGRSAPALSIVEGLAEALATTTEANTSTTCVPIAEQDCVRHICLEGQLIRVNQSQHCPQGAAPPRCGILGLAVRVGGDRCCPLWECACRCSIFPDLSFVTFDGSHVALFKEAIYILSQSPDEMLTVHVLDCKSANLGHLNWPPFCLVMLNMTHLAHQVTIDRFNRKVTVDLQPVWPPVSRYGFRIEDTGHMYMILTPSDIQIQWLHSSGLMIVEASKTSKAQGHGLCGICDGDAANDLTLKDGSVVGGAEDPAPFLDSWQVPSSLTSVGQTRFRPDSCATTDCSPCLRMVSNRTFSACHRFVPPESFCELWIRDTKYVQQPCVALTVYVAMCHKFHVCIEWRRSDYCPFLCSSDSTYQACVTACEPPKTCQDGILGPLDPEHCQVLGEGCVCSEGTILHRRHSALCIPEAKCACTDSMGVPRALGETWNSSLSGCCQHQCQAPDTIVPVDLGCPSPRPESCLRFGEVALLLPTKDPCCLGTVCVCNQTLCEGLAPTCRPGHRLLTHFQEDSCCPSYSCECDPDLCEAELVPSCRQDQILITGRLGDSCCTSYFCACGDCPDSIPECQEGEALTVHRNTTELCCPLYQCVCENFRCPQVQCGLGTALVEVWSPDRCCPYKSCECDCDTIPVPRCHLWEKSQLDEEFMHSVENVCGCAKYECVKAPVCLSRELGVMQPGQTVVELSADGVCHTSRCTTVLDPLTNFYQINTTSVLCDIHCEANQEYEHPRDLAACCGSCRNVSCLFTFPNGTTSLFLPGASWIADCARHHCSSTPLGAVLVRSPISCPPLNETECAKVGGSVVPSLEGCCRTCKEDGRSCKKVTIRMTIRKNECRSSTPVNLVSCDGRCPSASIYNYNINTYARFCKCCREVGLQRRSVQLFCATNATWVPYTVQEPTDCACQWS

Target ORF information:

RefSeq Version	NM_001292063.1
Organism	Homo sapiens(human)
Definition	Homo sapiens otogelin (OTOG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292063.1

ORF Insert Sequence:

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGCCGCAG CACCCGTTCT GTGGGGCAGT 
GCAGAGCCAC AGCCAGAGCC AGCCGGGCAA CCCAGCAGCA GCCACCAGGA GGCGACCCTT 
GCCATGGGGG ACAAGGCTAC AGTCGTGGGA GGCCAGCAGG CTGAAGCCCC AGACTCCGTG 
GCCATGTCTT CCTGGGAAAG GCGGCTCCAT CGGGCCAAGT GTGCACCATC CTACTTGTTC 
TCCTGCTTCA ATGGAGGCGA GTGTGTGCAC CCAGCCTTCT GTGACTGCAG ACGCTTCAAT 
GCCACTGGAC CGCGCTGCCA GATGGTGTAC AATGCCGGCC CTGAGAGGGA CAGCATTTGC 
CGGGCGTGGG GGCAGCACCA CGTGGAGACA TTTGATGGGC TCTACTACTA CCTCTCCGGA 
AAGGGCAGCT ACACCCTGGT GGGTCGCCAT GAGCCCGAGG GACAGAGCTT CTCCATCCAG 
GTACACAATG ACCCGCAGTG TGGCTCTTCA CCCTACACCT GCTCCAGGGC TGTCAGCCTC 
TTCTTTGTGG GTGAGCAGGA GATCCATCTG GCCAAGGAGG TCACCCATGG AGGCATGAGG 
GTCCAACTGC CACATGTCAT GGGGAGCGCG CGTCTGCAGC AGCTTGCCGG CTATGTCATC 
GTGCGGCATC AGTCAGCCTT CACACTGGCC TGGGATGGTG CCTCGGCTGT CTACATCAAG 
ATGAGTCCAG AGCTTCTGGG CTGGACCCAT GGGCTGTGTG GGAACAACAA TGCTGACCCC 
AAGGATGATC TGGTGACCAG CTCTGGGAAG CTGACTGACG ACGTGGTTGA GTTTGTGCAC 
AGCTGGCAGG AGCAGGCCCC TAACCAGCCT CCAGGGCCCA CAACTTCCTC CCTGCCTCGC 
CCACCGTGCC TACAGCAGAA CCCAGGAACC ATGCAGGGCG TGTACGAGCA GTGTGAGGCT 
CTACTGCGGC CCCCCTTTGA CGCCTGCCAC GCCTACGTCA GCCCTCTGCC CTTCACAGCC 
AGTTGTACCA GTGATCTCTG CCAATCAATG GGTGATGTAG CCACCTGGTG CCGGGCACTG 
GCGGAGTATG CCCGGGCGTG TGCCCAGGCA GGGCGGCCCT TGCAAGGCTG GAGGACCCAG 
CTCCGGCAAT GCACTGTGCA CTGCAAGGAG AAGGCCTTTA CCTACAATGA GTGCATCGCC 
TGCTGCCCTG CCTCCTGCCA TCCCCGGGCA TCCTGTGTGG ACAGTGAGAT CGCCTGTGTG 
GACGGCTGCT ATTGCCCCAA TGGGCTCATC TTCGAGGATG GGGGCTGCGT GGCACCAGCT 
GAGTGTCCCT GTGAGTTTCA CGGGACTCTG TACCCACCTG GCTCTGTGGT GAAGGAAGAC 
TGCAATACTT GCACATGCAC CTCAGGCAAG TGGGAGTGCA GCACAGCTGT CTGCCCAGCT 
GAGTGCTCAG TGACTGGTGA CATTCACTTC ACAACCTTTG ATGGCCGCCG GTACACGTTC 
CCCGCCACAT GTCAGTACAT CCTGGCCAAG AGCCGCTCTT CGGGCACCTT CACCGTGACA 
TTGCAGAATG CCCCATGTGG CCTGAACCAA GATGGAGCCT GTGTCCAGTC AGTGTCAGTG 
ATTCTGCACC AGGACCCTCG GAGGCAGGTG ACCCTGACCC AGGCAGGGGA TGTCCTTCTG 
TTTGACCAGT ACAAGATCAT CCCGCCATAC ACAGATGATG CCTTTGAGAT CCGTAGGCTG 
TCCTCCGTGT TCCTGCGGGT GAGGACGAAC GTGGGCGTGC GGGTGCTCTA CGACCGTGAA 
GGGCTCCGAC TGTACCTGCA AGTGGACCAG CGATGGGTGG AGGATACCGT GGGCCTCTGC 
GGCACCTTCA ATGGCAACAC GCAGGATGAC TTCCTGTCTC CAGTGGGTGT ACCTGAGAGC 
ACCCCACAAC TTTTTGGCAA TTCCTGGAAA ACACTTTCTG CTTGCTCCCC GCTGGTCTCT 
GGCTCCCCTC TGGACCCCTG CGATGTGCAC CTGCAAGCCG CCTCCTACTC AGTGCAGGCC 
TGCAGCGTGC TCACGGGGGA GATGTTTGCG CCCTGCTCTG CGTTCCTGAG CCCCGTGCCC 
TACTTTGAGC AGTGCCGCAG GGATGCCTGC CGCTGCGGGC AGCCCTGCCT GTGCGCCACA 
CTGGCCCACT ACGCCCACCT GTGCCGGCGC CATGGGCTCC CCGTTGATTT CCGCGCCCGC 
CTGCCAGCCT GTGCACTGTC CTGTGAGGCC TCCAAGGAGT ATAGCCCCTG CGTGGCCCCG 
TGTGGACGTA CCTGCCAGGA CCTGGCCAGC CCTGAGGCCT GTGGGGTTGA TGGTGGCGAT 
GACCTGAGCA GAGACGAGTG TGTGGAGGGC TGTGCCTGCC CACCGGACAC CTATCTGGAC 
ACCCAGGCTG ACCTCTGTGT CCCCCGGAAC CAGTGCTCCT GCCACTTCCA GGGAGTGGAC 
TATCCCCCCG GAGACAGTGA CATCCCATCC CTGGGCCACT GCCACTGCAA AGATGGAGTC 
ATGAGCTGTG ATAGCAGAGC CCCAGCTGCT GCCTGCCCAG CAGGCCAGGT CTTCGTGAAC 
TGCAGCGACC TGCACACGGA CCTGGAGCTG AGCAGGGAGA GGACGTGTGA GCAGCAACTG 
CTGAACCTGA GCGTGTCAGC CCGTGGCCCC TGCCTCTCGG GCTGCGCCTG TCCCCAGGGT 
CTGCTCAGAC ACGGGGATGC ATGTTTCCTG CCAGAGGAGT GCCCCTGCAC TTGGAAGGGG 
AAGGAGTATT TCCCTGGGGA CCAGGTGATG TCTCCTTGCC ATACCTGTGT GTGCCAGCGG 
GGCTCATTCC AGTGCACCCT GCACCCTTGC GCCTCCACCT GCACTGCCTA TGGGGACCGG 
CATTACCGCA CGTTTGATGG GCTCCCGTTT GACTTCGTGG GGGCATGCAA AGTGCACCTG 
GTCAAGAGCA CATCAGATGT CAGCTTCTCT GTGATTGTAG AGAATGTGAA CTGCTACAGC 
TCTGGCATGA TCTGCAGGAA ATTTATTTCC ATCAACGTTG GGAACTCACT CATTGTCTTT 
GATGATGACT CCGGAAATCC TAGTCCAGAG AGCTTCCTGG ATGACAAGCA GGAGGTCCAC 
ACATGGCGAG TGGGATTTTT CACACTGGTG CATTTCCCAC AGGAGCACAT CACCCTCTTG 
TGGGACCAGA GAACCACAGT GCACGTCCAG GCTGGGCCTC AGTGGCAGGG CCAGCTGGCG 
GGCCTCTGTG GGAACTTTGA CTTAAAAACC ATCAATGAGA TGAGGACCCC GGAGAACCTA 
GAGCTAACTA ACCCCCAGGA GTTTGGCAGC AGTTGGGCTG CAGTTGAGTG CCCAGACACC 
CTCGATCCTC GGGATATGTG TGTCCTGAAT CCTCTCCGAG AACCATTTGC CAAGAAGGAG 
TGCAGCATCC TGCTCAGTGA GGTGTTTGAG ATCTGCCACC CTGTGGTTGA TGTCACTTGG 
TTTTACTCAA ACTGCCTGAC AGACACATGT GGCTGCAGCC AGGGTGGTGA CTGTGAGTGC 
TTCTGTGCCA GCGTCTCCGC TTATGCCCAC CAGTGTTGCC AGCATGGGGT GGCTGTTGAC 
TGGCGAACCC CCCGCCTCTG CCCGTATGAC TGTGACTTCT TTAACAAAGT GCTAGGTAAG 
GGCCCCTATC AGCTATCCAG CTTGGCAGCC GGTGGTGCTC TGGTGGGCAT GAAGGCGGTG 
GGCGATGACA TAGTCCTAGT GAGGACAGAG GATGTGGCGC CAGCAGACAT TGTGAGCTTC 
CTGCTGACAG CTGCTCTGTA CAAGGCCAAG GCCCATGACC CAGATGTGGT GTCCCTGGAG 
GCAGCAGACA GACCCAACTT CTTCCTTCAC GTCACAGCCA ACGGGTCTCT GGAGCTGGCT 
AAGTGGCAGG GCCGTGACAC CTTCCAACAG CATGCCTCCT TCTTGCTGCA CCGGGGGACA 
CGGCAGGCAG GCCTGGTGGC CCTGGAGTCC CTGGCCAAGC CCAGCTCCTT CCTCTATGTG 
TCGGGCGCGG TGCTGGCCCT GCGGCTGTAC GAACACACAG AGGTGTTCCG CCGGGGCACA 
CTCTTCCGCC TTCTGGATGC CAAGCCCTCG GGGGCTGCCT ACCCCATCTG CGAGTGGCGC 
TACGATGCCT GTGCCAGCCC CTGCTTCCAA ACCTGCCGGG ACCCACGGGC AGCCAGCTGC 
CGGGACGTAC CCAGGGTAGA AGGCTGTGTC CCTGTGTGCC CCACCCCCCA GGTCCTGGAT 
GAAGTCACAC AGAGATGTGT CTACTTGGAG GACTGTGTGG AGCCAGCAGT TTGGGTTCCC 
ACAGAGGCCC TTGGCAATGA GACCCTCCCT CCCAGTCAAG GGTTGCCCAC TCCCAGTGAT 
GAGGAGCCAC AGCTGTCACA GGAAAGCCCC AGGACCCCCA CCCACAGGCC AGCCCTCACC 
CCAGCTGCCC CACTCACCAC AGCCCTGAAC CCACCAGTGA CAGCCACTGA GGAGCCAGTG 
GTGTCTCCAG GCCCCACCCA GACCACCCTG CAGCAGCCAC TGGAGCTCAC TGCATCTCAA 
CTCCCCGCCG GCCCCACGGA GTCCCCAGCC AGCAAGGGAG TGACTGCCAG CCTCCTGGCC 
ATCCCCCATA CACCAGAGTC CTCATCCCTC CCTGTTGCAC TGCAGACACC CACACCTGGC 
ATGGTGTCAG GTGCCATGGA GACAACAAGG GTGACTGTGA TCTTTGCAGG AAGCCCTAAC 
ATCACAGTCT CCTCCCGGTC GCCCCCTGCC CCTCGCTTCC CGCTCATGAC CAAGGCTGTG 
ACAGTCCGAG GCCATGGCTC CTTGCCTGTT AGGACGACAC CCCCACAGCC CTCCTTGACA 
GCAAGTCCCT CCTCCAGACC TGTGGCTTCC CCTGGAGCCA TCTCCAGGTC CCCCACCTCC 
TCGGGATCCC ACAAGGCTGT GCTGACACCT GCAGTAACTA AGGTCATAAG CAGGACAGGG 
GTCCCCCAGC CCACCCAGGC CCAGAGTGCT TCAAGTCCCA GCACCCCTCT AACTGTGGCT 
GGAACAGCAG CAGAACAGGT TCCTGTCAGT CCCCTTGCAA CCAGGAGCTT GGAGATAGTG 
CTATCCACAG AGAAGGGCGA AGCCGGGCAC AGCCAGCCCA TGGGCTCGCC TGCCTCCCCA 
CAGCCACACC CACTCCCCTC TGCACCACCC CGCCCAGCCC AGCATACCAC CATGGCCACC 
AGGTCTCCAG CTCTGCCCCC AGAGACCCCA GCTGCCGCCA GCCTGTCAAC AGCCACTGAT 
GGGCTGGCAG CCACACCCTT CATGTCCCTT GAGTCAACTC GTCCCTCCCA GCTCCTCTCT 
GGCCTGCCTC CCGACACCAG CCTGCCCCTG GCCAAGGTGG GCACATCTGC CCCAGTGGCC 
ACACCCGGCC CCAAAGCCTC TGTCATCACC ACTCCACTCC AGCCACAGGC CACGACTCTG 
CCTGCTCAGA CACTTAGCCC AGTACTGCCT TTCACTCCAG CAGCAATGAC CCAGGCGCAC 
CCACCCACTC ACATAGCACC CCCAGCAGCA GGCACAGCTC CAGGCCTGCT GCTGGGAGCC 
ACATTGCCAA CCTCTGGAGT CCTGCCTGTG GCTGAGGGCA CGGCCTCCAT GGTATCTGTT 
GTCCCACGAA AGAGCACCAC AGGGAAGGTG GCCATCCTAT CCAAGCAAGT GTCTCTGCCC 
ACTTCCATGT ATGGTTCTGC AGAGGGTGGG CCCACAGAGC TCACGCCTGC TACGAGCCAC 
CCTCTCACGC CCTTGGTGGC TGAGCCCGAG GGAGCCCAGG CAGGCACAGC TCTGCCAGTG 
CCCACATCCT ATGCCCTGAG CCGTGTCTCA GCCAGGACGG CCCCCCAAGA CAGCATGCTG 
GTTCTGTTGC CTCAGCTGGC TGAGGCCCAT GGAACCTCGG CAGGGCCTCA CCTGGCAGCA 
GAGCCGGTGG ACGAGGCCAC CACAGAACCA TCTGGGCGCT CAGCCCCAGC CCTGAGCATC 
GTAGAGGGTT TGGCGGAGGC TTTGGCAACT ACCACTGAGG CCAATACATC CACCACCTGT 
GTTCCAATCG CCGAGCAGGA CTGCGTCCGC CACATCTGCC TGGAGGGCCA GCTGATTCGC 
GTGAATCAGT CCCAGCACTG TCCCCAGGGT GCTGCTCCCC CTCGCTGTGG GATCCTGGGC 
CTCGCCGTGC GGGTGGGTGG GGACCGCTGC TGCCCACTCT GGGAGTGTGC CTGCCGGTGC 
TCAATCTTCC CTGACCTGAG CTTCGTGACC TTCGATGGGA GCCACGTAGC TCTGTTCAAG 
GAGGCCATCT ACATCCTCAG CCAGAGCCCA GATGAAATGC TCACCGTCCA TGTACTGGAC 
TGCAAAAGTG CCAACCTGGG GCACCTGAAC TGGCCCCCGT TCTGTCTGGT GATGTTGAAC 
ATGACTCACT TGGCCCATCA GGTCACTATT GATCGCTTCA ACCGAAAGGT GACTGTGGAC 
TTGCAGCCTG TGTGGCCACC GGTGAGCAGG TATGGATTCA GAATTGAGGA CACAGGCCAC 
ATGTACATGA TCCTGACTCC CTCAGACATC CAGATCCAGT GGCTCCACAG CTCAGGACTC 
ATGATCGTGG AGGCCAGCAA AACCAGCAAG GCCCAGGGCC ATGGCCTGTG CGGTATCTGT 
GATGGAGATG CAGCCAATGA CCTTACCCTG AAGGATGGCT CAGTGGTGGG TGGGGCTGAG 
GACCCTGCTC CCTTTCTGGA CAGCTGGCAG GTGCCCAGCT CCCTGACCTC AGTGGGCCAG 
ACCCGCTTCC GCCCAGACAG CTGCGCCACA ACTGACTGCT CGCCCTGCCT TCGCATGGTG 
TCCAACCGCA CCTTCAGTGC CTGCCACCGC TTTGTGCCTC CGGAGTCATT CTGTGAGCTG 
TGGATCCGGG ACACCAAGTA CGTGCAGCAG CCCTGCGTGG CCCTGACTGT GTACGTGGCC 
ATGTGCCACA AATTTCATGT GTGCATCGAG TGGCGGCGCT CTGACTACTG CCCCTTCCTG 
TGCTCCAGCG ACTCCACATA CCAGGCATGT GTGACAGCCT GTGAGCCACC CAAGACATGC 
CAGGATGGGA TACTAGGGCC TCTGGACCCA GAGCACTGCC AGGTGCTGGG CGAGGGCTGC 
GTCTGCTCCG AGGGCACCAT CTTACACCGG CGCCACTCTG CACTCTGCAT CCCGGAGGCC 
AAGTGCGCCT GCACTGACAG CATGGGGGTG CCGAGGGCCC TGGGGGAGAC CTGGAACAGC 
TCCCTCAGCG GCTGCTGCCA GCACCAGTGC CAAGCCCCAG ACACCATTGT CCCGGTGGAT 
CTGGGCTGCC CCAGTCCCCG CCCTGAGAGC TGCCTGCGAT TCGGGGAGGT GGCCTTGCTC 
CTACCCACCA AGGACCCCTG CTGCCTGGGG ACTGTCTGTG TGTGTAACCA GACTCTGTGT 
GAGGGTCTCG CCCCCACATG CCGCCCAGGC CACCGCCTCC TCACCCACTT CCAGGAGGAC 
TCCTGCTGCC CCAGCTACAG CTGTGAGTGT GACCCAGATC TCTGTGAGGC AGAGCTGGTC 
CCCAGCTGCC GACAGGACCA GATCCTGATC ACGGGCCGCC TGGGGGACTC CTGCTGCACC 
TCCTACTTCT GCGCCTGTGG TGACTGTCCA GACTCCATCC CCGAATGTCA AGAAGGGGAG 
GCGCTCACTG TGCACAGGAA TACCACGGAA CTCTGCTGCC CTCTGTACCA GTGTGTGTGT 
GAGAACTTCC GCTGTCCCCA AGTGCAGTGT GGCCTGGGCA CTGCCCTGGT GGAGGTGTGG 
AGCCCCGACC GCTGCTGCCC CTACAAATCC TGTGAATGTG ACTGTGACAC AATCCCGGTG 
CCCCGGTGCC ATCTGTGGGA GAAATCCCAG CTGGATGAGG AGTTCATGCA CAGCGTGGAG 
AATGTGTGTG GCTGCGCCAA GTACGAGTGT GTGAAGGCCC CGGTGTGTCT GAGCCGCGAG 
CTGGGTGTGA TGCAGCCCGG CCAGACAGTG GTGGAGCTCT CAGCAGATGG CGTGTGCCAC 
ACCTCCCGCT GCACCACCGT GCTCGACCCT CTCACCAACT TCTACCAGAT CAACACCACC 
TCCGTGCTCT GTGACATCCA CTGTGAGGCG AACCAGGAGT ACGAGCACCC GCGGGACCTC 
GCTGCCTGCT GCGGCTCCTG CAGGAACGTG TCCTGTCTCT TCACCTTCCC CAATGGCACC 
ACCTCCCTGT TCTTGCCCGG GGCATCCTGG ATCGCAGACT GCGCCCGCCA CCACTGCAGC 
AGCACGCCCC TGGGTGCCGT GCTGGTCCGC TCTCCCATAA GCTGCCCACC GCTCAATGAG 
ACTGAGTGTG CCAAGGTTGG GGGTTCCGTG GTACCTTCCT TGGAAGGATG CTGCAGGACC 
TGTAAGGAGG ATGGGCGCTC CTGCAAGAAG GTGACCATCC GCATGACCAT CCGCAAGAAT 
GAATGCAGGA GCAGCACCCC TGTGAACCTA GTGTCCTGCG ATGGGAGGTG CCCATCCGCC 
AGCATCTACA ACTACAACAT CAACACCTAT GCCCGATTCT GCAAGTGCTG CCGTGAGGTG 
GGCCTGCAGC GGCGCTCTGT GCAGCTCTTC TGTGCCACCA ATGCCACCTG GGTGCCCTAT 
ACAGTGCAGG AGCCCACCGA CTGTGCCTGC CAGTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOG ( NM_001292063.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001278992.1 Homo sapiens otogelin (OTOG), transcript variant 2, mRNA.

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CloneID	OHu25668
Clone ID Related Accession (Same CDS sequence)	NM_001292063.1 , NM_001292063.2
Accession Version	NM_001292063.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	otogelin isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124799.5 and KC877393.1. On Sep 3, 2019 this sequence version replaced NM_001292063.1. Transcript Variant: This variant (2) uses two alternate splice sites and includes an additional exon, which together result in an alternate in-frame segment in the 5' coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS representation lacks full-length human transcript support. Its exon combination is therefore inferred, but it is supported by the full-length orthologous mouse transcript U96411.1, by partial human transcript alignments, and by data in PMID:23122587. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END##

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGCCGCAG CACCCGTTCT GTGGGGCAGT 
GCAGAGCCAC AGCCAGAGCC AGCCGGGCAA CCCAGCAGCA GCCACCAGGA GGCGACCCTT 
GCCATGGGGG ACAAGGCTAC AGTCGTGGGA GGCCAGCAGG CTGAAGCCCC AGACTCCGTG 
GCCATGTCTT CCTGGGAAAG GCGGCTCCAT CGGGCCAAGT GTGCACCATC CTACTTGTTC 
TCCTGCTTCA ATGGAGGCGA GTGTGTGCAC CCAGCCTTCT GTGACTGCAG ACGCTTCAAT 
GCCACTGGAC CGCGCTGCCA GATGGTGTAC AATGCCGGCC CTGAGAGGGA CAGCATTTGC 
CGGGCGTGGG GGCAGCACCA CGTGGAGACA TTTGATGGGC TCTACTACTA CCTCTCCGGA 
AAGGGCAGCT ACACCCTGGT GGGTCGCCAT GAGCCCGAGG GACAGAGCTT CTCCATCCAG 
GTACACAATG ACCCGCAGTG TGGCTCTTCA CCCTACACCT GCTCCAGGGC TGTCAGCCTC 
TTCTTTGTGG GTGAGCAGGA GATCCATCTG GCCAAGGAGG TCACCCATGG AGGCATGAGG 
GTCCAACTGC CACATGTCAT GGGGAGCGCG CGTCTGCAGC AGCTTGCCGG CTATGTCATC 
GTGCGGCATC AGTCAGCCTT CACACTGGCC TGGGATGGTG CCTCGGCTGT CTACATCAAG 
ATGAGTCCAG AGCTTCTGGG CTGGACCCAT GGGCTGTGTG GGAACAACAA TGCTGACCCC 
AAGGATGATC TGGTGACCAG CTCTGGGAAG CTGACTGACG ACGTGGTTGA GTTTGTGCAC 
AGCTGGCAGG AGCAGGCCCC TAACCAGCCT CCAGGGCCCA CAACTTCCTC CCTGCCTCGC 
CCACCGTGCC TACAGCAGAA CCCAGGAACC ATGCAGGGCG TGTACGAGCA GTGTGAGGCT 
CTACTGCGGC CCCCCTTTGA CGCCTGCCAC GCCTACGTCA GCCCTCTGCC CTTCACAGCC 
AGTTGTACCA GTGATCTCTG CCAATCAATG GGTGATGTAG CCACCTGGTG CCGGGCACTG 
GCGGAGTATG CCCGGGCGTG TGCCCAGGCA GGGCGGCCCT TGCAAGGCTG GAGGACCCAG 
CTCCGGCAAT GCACTGTGCA CTGCAAGGAG AAGGCCTTTA CCTACAATGA GTGCATCGCC 
TGCTGCCCTG CCTCCTGCCA TCCCCGGGCA TCCTGTGTGG ACAGTGAGAT CGCCTGTGTG 
GACGGCTGCT ATTGCCCCAA TGGGCTCATC TTCGAGGATG GGGGCTGCGT GGCACCAGCT 
GAGTGTCCCT GTGAGTTTCA CGGGACTCTG TACCCACCTG GCTCTGTGGT GAAGGAAGAC 
TGCAATACTT GCACATGCAC CTCAGGCAAG TGGGAGTGCA GCACAGCTGT CTGCCCAGCT 
GAGTGCTCAG TGACTGGTGA CATTCACTTC ACAACCTTTG ATGGCCGCCG GTACACGTTC 
CCCGCCACAT GTCAGTACAT CCTGGCCAAG AGCCGCTCTT CGGGCACCTT CACCGTGACA 
TTGCAGAATG CCCCATGTGG CCTGAACCAA GATGGAGCCT GTGTCCAGTC AGTGTCAGTG 
ATTCTGCACC AGGACCCTCG GAGGCAGGTG ACCCTGACCC AGGCAGGGGA TGTCCTTCTG 
TTTGACCAGT ACAAGATCAT CCCGCCATAC ACAGATGATG CCTTTGAGAT CCGTAGGCTG 
TCCTCCGTGT TCCTGCGGGT GAGGACGAAC GTGGGCGTGC GGGTGCTCTA CGACCGTGAA 
GGGCTCCGAC TGTACCTGCA AGTGGACCAG CGATGGGTGG AGGATACCGT GGGCCTCTGC 
GGCACCTTCA ATGGCAACAC GCAGGATGAC TTCCTGTCTC CAGTGGGTGT ACCTGAGAGC 
ACCCCACAAC TTTTTGGCAA TTCCTGGAAA ACACTTTCTG CTTGCTCCCC GCTGGTCTCT 
GGCTCCCCTC TGGACCCCTG CGATGTGCAC CTGCAAGCCG CCTCCTACTC AGTGCAGGCC 
TGCAGCGTGC TCACGGGGGA GATGTTTGCG CCCTGCTCTG CGTTCCTGAG CCCCGTGCCC 
TACTTTGAGC AGTGCCGCAG GGATGCCTGC CGCTGCGGGC AGCCCTGCCT GTGCGCCACA 
CTGGCCCACT ACGCCCACCT GTGCCGGCGC CATGGGCTCC CCGTTGATTT CCGCGCCCGC 
CTGCCAGCCT GTGCACTGTC CTGTGAGGCC TCCAAGGAGT ATAGCCCCTG CGTGGCCCCG 
TGTGGACGTA CCTGCCAGGA CCTGGCCAGC CCTGAGGCCT GTGGGGTTGA TGGTGGCGAT 
GACCTGAGCA GAGACGAGTG TGTGGAGGGC TGTGCCTGCC CACCGGACAC CTATCTGGAC 
ACCCAGGCTG ACCTCTGTGT CCCCCGGAAC CAGTGCTCCT GCCACTTCCA GGGAGTGGAC 
TATCCCCCCG GAGACAGTGA CATCCCATCC CTGGGCCACT GCCACTGCAA AGATGGAGTC 
ATGAGCTGTG ATAGCAGAGC CCCAGCTGCT GCCTGCCCAG CAGGCCAGGT CTTCGTGAAC 
TGCAGCGACC TGCACACGGA CCTGGAGCTG AGCAGGGAGA GGACGTGTGA GCAGCAACTG 
CTGAACCTGA GCGTGTCAGC CCGTGGCCCC TGCCTCTCGG GCTGCGCCTG TCCCCAGGGT 
CTGCTCAGAC ACGGGGATGC ATGTTTCCTG CCAGAGGAGT GCCCCTGCAC TTGGAAGGGG 
AAGGAGTATT TCCCTGGGGA CCAGGTGATG TCTCCTTGCC ATACCTGTGT GTGCCAGCGG 
GGCTCATTCC AGTGCACCCT GCACCCTTGC GCCTCCACCT GCACTGCCTA TGGGGACCGG 
CATTACCGCA CGTTTGATGG GCTCCCGTTT GACTTCGTGG GGGCATGCAA AGTGCACCTG 
GTCAAGAGCA CATCAGATGT CAGCTTCTCT GTGATTGTAG AGAATGTGAA CTGCTACAGC 
TCTGGCATGA TCTGCAGGAA ATTTATTTCC ATCAACGTTG GGAACTCACT CATTGTCTTT 
GATGATGACT CCGGAAATCC TAGTCCAGAG AGCTTCCTGG ATGACAAGCA GGAGGTCCAC 
ACATGGCGAG TGGGATTTTT CACACTGGTG CATTTCCCAC AGGAGCACAT CACCCTCTTG 
TGGGACCAGA GAACCACAGT GCACGTCCAG GCTGGGCCTC AGTGGCAGGG CCAGCTGGCG 
GGCCTCTGTG GGAACTTTGA CTTAAAAACC ATCAATGAGA TGAGGACCCC GGAGAACCTA 
GAGCTAACTA ACCCCCAGGA GTTTGGCAGC AGTTGGGCTG CAGTTGAGTG CCCAGACACC 
CTCGATCCTC GGGATATGTG TGTCCTGAAT CCTCTCCGAG AACCATTTGC CAAGAAGGAG 
TGCAGCATCC TGCTCAGTGA GGTGTTTGAG ATCTGCCACC CTGTGGTTGA TGTCACTTGG 
TTTTACTCAA ACTGCCTGAC AGACACATGT GGCTGCAGCC AGGGTGGTGA CTGTGAGTGC 
TTCTGTGCCA GCGTCTCCGC TTATGCCCAC CAGTGTTGCC AGCATGGGGT GGCTGTTGAC 
TGGCGAACCC CCCGCCTCTG CCCGTATGAC TGTGACTTCT TTAACAAAGT GCTAGGTAAG 
GGCCCCTATC AGCTATCCAG CTTGGCAGCC GGTGGTGCTC TGGTGGGCAT GAAGGCGGTG 
GGCGATGACA TAGTCCTAGT GAGGACAGAG GATGTGGCGC CAGCAGACAT TGTGAGCTTC 
CTGCTGACAG CTGCTCTGTA CAAGGCCAAG GCCCATGACC CAGATGTGGT GTCCCTGGAG 
GCAGCAGACA GACCCAACTT CTTCCTTCAC GTCACAGCCA ACGGGTCTCT GGAGCTGGCT 
AAGTGGCAGG GCCGTGACAC CTTCCAACAG CATGCCTCCT TCTTGCTGCA CCGGGGGACA 
CGGCAGGCAG GCCTGGTGGC CCTGGAGTCC CTGGCCAAGC CCAGCTCCTT CCTCTATGTG 
TCGGGCGCGG TGCTGGCCCT GCGGCTGTAC GAACACACAG AGGTGTTCCG CCGGGGCACA 
CTCTTCCGCC TTCTGGATGC CAAGCCCTCG GGGGCTGCCT ACCCCATCTG CGAGTGGCGC 
TACGATGCCT GTGCCAGCCC CTGCTTCCAA ACCTGCCGGG ACCCACGGGC AGCCAGCTGC 
CGGGACGTAC CCAGGGTAGA AGGCTGTGTC CCTGTGTGCC CCACCCCCCA GGTCCTGGAT 
GAAGTCACAC AGAGATGTGT CTACTTGGAG GACTGTGTGG AGCCAGCAGT TTGGGTTCCC 
ACAGAGGCCC TTGGCAATGA GACCCTCCCT CCCAGTCAAG GGTTGCCCAC TCCCAGTGAT 
GAGGAGCCAC AGCTGTCACA GGAAAGCCCC AGGACCCCCA CCCACAGGCC AGCCCTCACC 
CCAGCTGCCC CACTCACCAC AGCCCTGAAC CCACCAGTGA CAGCCACTGA GGAGCCAGTG 
GTGTCTCCAG GCCCCACCCA GACCACCCTG CAGCAGCCAC TGGAGCTCAC TGCATCTCAA 
CTCCCCGCCG GCCCCACGGA GTCCCCAGCC AGCAAGGGAG TGACTGCCAG CCTCCTGGCC 
ATCCCCCATA CACCAGAGTC CTCATCCCTC CCTGTTGCAC TGCAGACACC CACACCTGGC 
ATGGTGTCAG GTGCCATGGA GACAACAAGG GTGACTGTGA TCTTTGCAGG AAGCCCTAAC 
ATCACAGTCT CCTCCCGGTC GCCCCCTGCC CCTCGCTTCC CGCTCATGAC CAAGGCTGTG 
ACAGTCCGAG GCCATGGCTC CTTGCCTGTT AGGACGACAC CCCCACAGCC CTCCTTGACA 
GCAAGTCCCT CCTCCAGACC TGTGGCTTCC CCTGGAGCCA TCTCCAGGTC CCCCACCTCC 
TCGGGATCCC ACAAGGCTGT GCTGACACCT GCAGTAACTA AGGTCATAAG CAGGACAGGG 
GTCCCCCAGC CCACCCAGGC CCAGAGTGCT TCAAGTCCCA GCACCCCTCT AACTGTGGCT 
GGAACAGCAG CAGAACAGGT TCCTGTCAGT CCCCTTGCAA CCAGGAGCTT GGAGATAGTG 
CTATCCACAG AGAAGGGCGA AGCCGGGCAC AGCCAGCCCA TGGGCTCGCC TGCCTCCCCA 
CAGCCACACC CACTCCCCTC TGCACCACCC CGCCCAGCCC AGCATACCAC CATGGCCACC 
AGGTCTCCAG CTCTGCCCCC AGAGACCCCA GCTGCCGCCA GCCTGTCAAC AGCCACTGAT 
GGGCTGGCAG CCACACCCTT CATGTCCCTT GAGTCAACTC GTCCCTCCCA GCTCCTCTCT 
GGCCTGCCTC CCGACACCAG CCTGCCCCTG GCCAAGGTGG GCACATCTGC CCCAGTGGCC 
ACACCCGGCC CCAAAGCCTC TGTCATCACC ACTCCACTCC AGCCACAGGC CACGACTCTG 
CCTGCTCAGA CACTTAGCCC AGTACTGCCT TTCACTCCAG CAGCAATGAC CCAGGCGCAC 
CCACCCACTC ACATAGCACC CCCAGCAGCA GGCACAGCTC CAGGCCTGCT GCTGGGAGCC 
ACATTGCCAA CCTCTGGAGT CCTGCCTGTG GCTGAGGGCA CGGCCTCCAT GGTATCTGTT 
GTCCCACGAA AGAGCACCAC AGGGAAGGTG GCCATCCTAT CCAAGCAAGT GTCTCTGCCC 
ACTTCCATGT ATGGTTCTGC AGAGGGTGGG CCCACAGAGC TCACGCCTGC TACGAGCCAC 
CCTCTCACGC CCTTGGTGGC TGAGCCCGAG GGAGCCCAGG CAGGCACAGC TCTGCCAGTG 
CCCACATCCT ATGCCCTGAG CCGTGTCTCA GCCAGGACGG CCCCCCAAGA CAGCATGCTG 
GTTCTGTTGC CTCAGCTGGC TGAGGCCCAT GGAACCTCGG CAGGGCCTCA CCTGGCAGCA 
GAGCCGGTGG ACGAGGCCAC CACAGAACCA TCTGGGCGCT CAGCCCCAGC CCTGAGCATC 
GTAGAGGGTT TGGCGGAGGC TTTGGCAACT ACCACTGAGG CCAATACATC CACCACCTGT 
GTTCCAATCG CCGAGCAGGA CTGCGTCCGC CACATCTGCC TGGAGGGCCA GCTGATTCGC 
GTGAATCAGT CCCAGCACTG TCCCCAGGGT GCTGCTCCCC CTCGCTGTGG GATCCTGGGC 
CTCGCCGTGC GGGTGGGTGG GGACCGCTGC TGCCCACTCT GGGAGTGTGC CTGCCGGTGC 
TCAATCTTCC CTGACCTGAG CTTCGTGACC TTCGATGGGA GCCACGTAGC TCTGTTCAAG 
GAGGCCATCT ACATCCTCAG CCAGAGCCCA GATGAAATGC TCACCGTCCA TGTACTGGAC 
TGCAAAAGTG CCAACCTGGG GCACCTGAAC TGGCCCCCGT TCTGTCTGGT GATGTTGAAC 
ATGACTCACT TGGCCCATCA GGTCACTATT GATCGCTTCA ACCGAAAGGT GACTGTGGAC 
TTGCAGCCTG TGTGGCCACC GGTGAGCAGG TATGGATTCA GAATTGAGGA CACAGGCCAC 
ATGTACATGA TCCTGACTCC CTCAGACATC CAGATCCAGT GGCTCCACAG CTCAGGACTC 
ATGATCGTGG AGGCCAGCAA AACCAGCAAG GCCCAGGGCC ATGGCCTGTG CGGTATCTGT 
GATGGAGATG CAGCCAATGA CCTTACCCTG AAGGATGGCT CAGTGGTGGG TGGGGCTGAG 
GACCCTGCTC CCTTTCTGGA CAGCTGGCAG GTGCCCAGCT CCCTGACCTC AGTGGGCCAG 
ACCCGCTTCC GCCCAGACAG CTGCGCCACA ACTGACTGCT CGCCCTGCCT TCGCATGGTG 
TCCAACCGCA CCTTCAGTGC CTGCCACCGC TTTGTGCCTC CGGAGTCATT CTGTGAGCTG 
TGGATCCGGG ACACCAAGTA CGTGCAGCAG CCCTGCGTGG CCCTGACTGT GTACGTGGCC 
ATGTGCCACA AATTTCATGT GTGCATCGAG TGGCGGCGCT CTGACTACTG CCCCTTCCTG 
TGCTCCAGCG ACTCCACATA CCAGGCATGT GTGACAGCCT GTGAGCCACC CAAGACATGC 
CAGGATGGGA TACTAGGGCC TCTGGACCCA GAGCACTGCC AGGTGCTGGG CGAGGGCTGC 
GTCTGCTCCG AGGGCACCAT CTTACACCGG CGCCACTCTG CACTCTGCAT CCCGGAGGCC 
AAGTGCGCCT GCACTGACAG CATGGGGGTG CCGAGGGCCC TGGGGGAGAC CTGGAACAGC 
TCCCTCAGCG GCTGCTGCCA GCACCAGTGC CAAGCCCCAG ACACCATTGT CCCGGTGGAT 
CTGGGCTGCC CCAGTCCCCG CCCTGAGAGC TGCCTGCGAT TCGGGGAGGT GGCCTTGCTC 
CTACCCACCA AGGACCCCTG CTGCCTGGGG ACTGTCTGTG TGTGTAACCA GACTCTGTGT 
GAGGGTCTCG CCCCCACATG CCGCCCAGGC CACCGCCTCC TCACCCACTT CCAGGAGGAC 
TCCTGCTGCC CCAGCTACAG CTGTGAGTGT GACCCAGATC TCTGTGAGGC AGAGCTGGTC 
CCCAGCTGCC GACAGGACCA GATCCTGATC ACGGGCCGCC TGGGGGACTC CTGCTGCACC 
TCCTACTTCT GCGCCTGTGG TGACTGTCCA GACTCCATCC CCGAATGTCA AGAAGGGGAG 
GCGCTCACTG TGCACAGGAA TACCACGGAA CTCTGCTGCC CTCTGTACCA GTGTGTGTGT 
GAGAACTTCC GCTGTCCCCA AGTGCAGTGT GGCCTGGGCA CTGCCCTGGT GGAGGTGTGG 
AGCCCCGACC GCTGCTGCCC CTACAAATCC TGTGAATGTG ACTGTGACAC AATCCCGGTG 
CCCCGGTGCC ATCTGTGGGA GAAATCCCAG CTGGATGAGG AGTTCATGCA CAGCGTGGAG 
AATGTGTGTG GCTGCGCCAA GTACGAGTGT GTGAAGGCCC CGGTGTGTCT GAGCCGCGAG 
CTGGGTGTGA TGCAGCCCGG CCAGACAGTG GTGGAGCTCT CAGCAGATGG CGTGTGCCAC 
ACCTCCCGCT GCACCACCGT GCTCGACCCT CTCACCAACT TCTACCAGAT CAACACCACC 
TCCGTGCTCT GTGACATCCA CTGTGAGGCG AACCAGGAGT ACGAGCACCC GCGGGACCTC 
GCTGCCTGCT GCGGCTCCTG CAGGAACGTG TCCTGTCTCT TCACCTTCCC CAATGGCACC 
ACCTCCCTGT TCTTGCCCGG GGCATCCTGG ATCGCAGACT GCGCCCGCCA CCACTGCAGC 
AGCACGCCCC TGGGTGCCGT GCTGGTCCGC TCTCCCATAA GCTGCCCACC GCTCAATGAG 
ACTGAGTGTG CCAAGGTTGG GGGTTCCGTG GTACCTTCCT TGGAAGGATG CTGCAGGACC 
TGTAAGGAGG ATGGGCGCTC CTGCAAGAAG GTGACCATCC GCATGACCAT CCGCAAGAAT 
GAATGCAGGA GCAGCACCCC TGTGAACCTA GTGTCCTGCG ATGGGAGGTG CCCATCCGCC 
AGCATCTACA ACTACAACAT CAACACCTAT GCCCGATTCT GCAAGTGCTG CCGTGAGGTG 
GGCCTGCAGC GGCGCTCTGT GCAGCTCTTC TGTGCCACCA ATGCCACCTG GGTGCCCTAT 
ACAGTGCAGG AGCCCACCGA CTGTGCCTGC CAGTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278992.1
CDS	115..8856
Translation	MGVLASALCWLLCVWLPWGEQAAESLRVQRLAAAPVLWGSAEPQPEPAGQPSSSHQEATLAMGDKATVVGGQQAEAPDSVAMSSWERRLHRAKCAPSYLFSCFNGGECVHPAFCDCRRFNATGPRCQMVYNAGPERDSICRAWGQHHVETFDGLYYYLSGKGSYTLVGRHEPEGQSFSIQVHNDPQCGSSPYTCSRAVSLFFVGEQEIHLAKEVTHGGMRVQLPHVMGSARLQQLAGYVIVRHQSAFTLAWDGASAVYIKMSPELLGWTHGLCGNNNADPKDDLVTSSGKLTDDVVEFVHSWQEQAPNQPPGPTTSSLPRPPCLQQNPGTMQGVYEQCEALLRPPFDACHAYVSPLPFTASCTSDLCQSMGDVATWCRALAEYARACAQAGRPLQGWRTQLRQCTVHCKEKAFTYNECIACCPASCHPRASCVDSEIACVDGCYCPNGLIFEDGGCVAPAECPCEFHGTLYPPGSVVKEDCNTCTCTSGKWECSTAVCPAECSVTGDIHFTTFDGRRYTFPATCQYILAKSRSSGTFTVTLQNAPCGLNQDGACVQSVSVILHQDPRRQVTLTQAGDVLLFDQYKIIPPYTDDAFEIRRLSSVFLRVRTNVGVRVLYDREGLRLYLQVDQRWVEDTVGLCGTFNGNTQDDFLSPVGVPESTPQLFGNSWKTLSACSPLVSGSPLDPCDVHLQAASYSVQACSVLTGEMFAPCSAFLSPVPYFEQCRRDACRCGQPCLCATLAHYAHLCRRHGLPVDFRARLPACALSCEASKEYSPCVAPCGRTCQDLASPEACGVDGGDDLSRDECVEGCACPPDTYLDTQADLCVPRNQCSCHFQGVDYPPGDSDIPSLGHCHCKDGVMSCDSRAPAAACPAGQVFVNCSDLHTDLELSRERTCEQQLLNLSVSARGPCLSGCACPQGLLRHGDACFLPEECPCTWKGKEYFPGDQVMSPCHTCVCQRGSFQCTLHPCASTCTAYGDRHYRTFDGLPFDFVGACKVHLVKSTSDVSFSVIVENVNCYSSGMICRKFISINVGNSLIVFDDDSGNPSPESFLDDKQEVHTWRVGFFTLVHFPQEHITLLWDQRTTVHVQAGPQWQGQLAGLCGNFDLKTINEMRTPENLELTNPQEFGSSWAAVECPDTLDPRDMCVLNPLREPFAKKECSILLSEVFEICHPVVDVTWFYSNCLTDTCGCSQGGDCECFCASVSAYAHQCCQHGVAVDWRTPRLCPYDCDFFNKVLGKGPYQLSSLAAGGALVGMKAVGDDIVLVRTEDVAPADIVSFLLTAALYKAKAHDPDVVSLEAADRPNFFLHVTANGSLELAKWQGRDTFQQHASFLLHRGTRQAGLVALESLAKPSSFLYVSGAVLALRLYEHTEVFRRGTLFRLLDAKPSGAAYPICEWRYDACASPCFQTCRDPRAASCRDVPRVEGCVPVCPTPQVLDEVTQRCVYLEDCVEPAVWVPTEALGNETLPPSQGLPTPSDEEPQLSQESPRTPTHRPALTPAAPLTTALNPPVTATEEPVVSPGPTQTTLQQPLELTASQLPAGPTESPASKGVTASLLAIPHTPESSSLPVALQTPTPGMVSGAMETTRVTVIFAGSPNITVSSRSPPAPRFPLMTKAVTVRGHGSLPVRTTPPQPSLTASPSSRPVASPGAISRSPTSSGSHKAVLTPAVTKVISRTGVPQPTQAQSASSPSTPLTVAGTAAEQVPVSPLATRSLEIVLSTEKGEAGHSQPMGSPASPQPHPLPSAPPRPAQHTTMATRSPALPPETPAAASLSTATDGLAATPFMSLESTRPSQLLSGLPPDTSLPLAKVGTSAPVATPGPKASVITTPLQPQATTLPAQTLSPVLPFTPAAMTQAHPPTHIAPPAAGTAPGLLLGATLPTSGVLPVAEGTASMVSVVPRKSTTGKVAILSKQVSLPTSMYGSAEGGPTELTPATSHPLTPLVAEPEGAQAGTALPVPTSYALSRVSARTAPQDSMLVLLPQLAEAHGTSAGPHLAAEPVDEATTEPSGRSAPALSIVEGLAEALATTTEANTSTTCVPIAEQDCVRHICLEGQLIRVNQSQHCPQGAAPPRCGILGLAVRVGGDRCCPLWECACRCSIFPDLSFVTFDGSHVALFKEAIYILSQSPDEMLTVHVLDCKSANLGHLNWPPFCLVMLNMTHLAHQVTIDRFNRKVTVDLQPVWPPVSRYGFRIEDTGHMYMILTPSDIQIQWLHSSGLMIVEASKTSKAQGHGLCGICDGDAANDLTLKDGSVVGGAEDPAPFLDSWQVPSSLTSVGQTRFRPDSCATTDCSPCLRMVSNRTFSACHRFVPPESFCELWIRDTKYVQQPCVALTVYVAMCHKFHVCIEWRRSDYCPFLCSSDSTYQACVTACEPPKTCQDGILGPLDPEHCQVLGEGCVCSEGTILHRRHSALCIPEAKCACTDSMGVPRALGETWNSSLSGCCQHQCQAPDTIVPVDLGCPSPRPESCLRFGEVALLLPTKDPCCLGTVCVCNQTLCEGLAPTCRPGHRLLTHFQEDSCCPSYSCECDPDLCEAELVPSCRQDQILITGRLGDSCCTSYFCACGDCPDSIPECQEGEALTVHRNTTELCCPLYQCVCENFRCPQVQCGLGTALVEVWSPDRCCPYKSCECDCDTIPVPRCHLWEKSQLDEEFMHSVENVCGCAKYECVKAPVCLSRELGVMQPGQTVVELSADGVCHTSRCTTVLDPLTNFYQINTTSVLCDIHCEANQEYEHPRDLAACCGSCRNVSCLFTFPNGTTSLFLPGASWIADCARHHCSSTPLGAVLVRSPISCPPLNETECAKVGGSVVPSLEGCCRTCKEDGRSCKKVTIRMTIRKNECRSSTPVNLVSCDGRCPSASIYNYNINTYARFCKCCREVGLQRRSVQLFCATNATWVPYTVQEPTDCACQWS

Target ORF information:

RefSeq Version	NM_001292063.2
Organism	Homo sapiens(human)
Definition	Homo sapiens otogelin (OTOG), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001292063.2

ORF Insert Sequence:

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGCCGCAG CACCCGTTCT GTGGGGCAGT 
GCAGAGCCAC AGCCAGAGCC AGCCGGGCAA CCCAGCAGCA GCCACCAGGA GGCGACCCTT 
GCCATGGGGG ACAAGGCTAC AGTCGTGGGA GGCCAGCAGG CTGAAGCCCC AGACTCCGTG 
GCCATGTCTT CCTGGGAAAG GCGGCTCCAT CGGGCCAAGT GTGCACCATC CTACTTGTTC 
TCCTGCTTCA ATGGAGGCGA GTGTGTGCAC CCAGCCTTCT GTGACTGCAG ACGCTTCAAT 
GCCACTGGAC CGCGCTGCCA GATGGTGTAC AATGCCGGCC CTGAGAGGGA CAGCATTTGC 
CGGGCGTGGG GGCAGCACCA CGTGGAGACA TTTGATGGGC TCTACTACTA CCTCTCCGGA 
AAGGGCAGCT ACACCCTGGT GGGTCGCCAT GAGCCCGAGG GACAGAGCTT CTCCATCCAG 
GTACACAATG ACCCGCAGTG TGGCTCTTCA CCCTACACCT GCTCCAGGGC TGTCAGCCTC 
TTCTTTGTGG GTGAGCAGGA GATCCATCTG GCCAAGGAGG TCACCCATGG AGGCATGAGG 
GTCCAACTGC CACATGTCAT GGGGAGCGCG CGTCTGCAGC AGCTTGCCGG CTATGTCATC 
GTGCGGCATC AGTCAGCCTT CACACTGGCC TGGGATGGTG CCTCGGCTGT CTACATCAAG 
ATGAGTCCAG AGCTTCTGGG CTGGACCCAT GGGCTGTGTG GGAACAACAA TGCTGACCCC 
AAGGATGATC TGGTGACCAG CTCTGGGAAG CTGACTGACG ACGTGGTTGA GTTTGTGCAC 
AGCTGGCAGG AGCAGGCCCC TAACCAGCCT CCAGGGCCCA CAACTTCCTC CCTGCCTCGC 
CCACCGTGCC TACAGCAGAA CCCAGGAACC ATGCAGGGCG TGTACGAGCA GTGTGAGGCT 
CTACTGCGGC CCCCCTTTGA CGCCTGCCAC GCCTACGTCA GCCCTCTGCC CTTCACAGCC 
AGTTGTACCA GTGATCTCTG CCAATCAATG GGTGATGTAG CCACCTGGTG CCGGGCACTG 
GCGGAGTATG CCCGGGCGTG TGCCCAGGCA GGGCGGCCCT TGCAAGGCTG GAGGACCCAG 
CTCCGGCAAT GCACTGTGCA CTGCAAGGAG AAGGCCTTTA CCTACAATGA GTGCATCGCC 
TGCTGCCCTG CCTCCTGCCA TCCCCGGGCA TCCTGTGTGG ACAGTGAGAT CGCCTGTGTG 
GACGGCTGCT ATTGCCCCAA TGGGCTCATC TTCGAGGATG GGGGCTGCGT GGCACCAGCT 
GAGTGTCCCT GTGAGTTTCA CGGGACTCTG TACCCACCTG GCTCTGTGGT GAAGGAAGAC 
TGCAATACTT GCACATGCAC CTCAGGCAAG TGGGAGTGCA GCACAGCTGT CTGCCCAGCT 
GAGTGCTCAG TGACTGGTGA CATTCACTTC ACAACCTTTG ATGGCCGCCG GTACACGTTC 
CCCGCCACAT GTCAGTACAT CCTGGCCAAG AGCCGCTCTT CGGGCACCTT CACCGTGACA 
TTGCAGAATG CCCCATGTGG CCTGAACCAA GATGGAGCCT GTGTCCAGTC AGTGTCAGTG 
ATTCTGCACC AGGACCCTCG GAGGCAGGTG ACCCTGACCC AGGCAGGGGA TGTCCTTCTG 
TTTGACCAGT ACAAGATCAT CCCGCCATAC ACAGATGATG CCTTTGAGAT CCGTAGGCTG 
TCCTCCGTGT TCCTGCGGGT GAGGACGAAC GTGGGCGTGC GGGTGCTCTA CGACCGTGAA 
GGGCTCCGAC TGTACCTGCA AGTGGACCAG CGATGGGTGG AGGATACCGT GGGCCTCTGC 
GGCACCTTCA ATGGCAACAC GCAGGATGAC TTCCTGTCTC CAGTGGGTGT ACCTGAGAGC 
ACCCCACAAC TTTTTGGCAA TTCCTGGAAA ACACTTTCTG CTTGCTCCCC GCTGGTCTCT 
GGCTCCCCTC TGGACCCCTG CGATGTGCAC CTGCAAGCCG CCTCCTACTC AGTGCAGGCC 
TGCAGCGTGC TCACGGGGGA GATGTTTGCG CCCTGCTCTG CGTTCCTGAG CCCCGTGCCC 
TACTTTGAGC AGTGCCGCAG GGATGCCTGC CGCTGCGGGC AGCCCTGCCT GTGCGCCACA 
CTGGCCCACT ACGCCCACCT GTGCCGGCGC CATGGGCTCC CCGTTGATTT CCGCGCCCGC 
CTGCCAGCCT GTGCACTGTC CTGTGAGGCC TCCAAGGAGT ATAGCCCCTG CGTGGCCCCG 
TGTGGACGTA CCTGCCAGGA CCTGGCCAGC CCTGAGGCCT GTGGGGTTGA TGGTGGCGAT 
GACCTGAGCA GAGACGAGTG TGTGGAGGGC TGTGCCTGCC CACCGGACAC CTATCTGGAC 
ACCCAGGCTG ACCTCTGTGT CCCCCGGAAC CAGTGCTCCT GCCACTTCCA GGGAGTGGAC 
TATCCCCCCG GAGACAGTGA CATCCCATCC CTGGGCCACT GCCACTGCAA AGATGGAGTC 
ATGAGCTGTG ATAGCAGAGC CCCAGCTGCT GCCTGCCCAG CAGGCCAGGT CTTCGTGAAC 
TGCAGCGACC TGCACACGGA CCTGGAGCTG AGCAGGGAGA GGACGTGTGA GCAGCAACTG 
CTGAACCTGA GCGTGTCAGC CCGTGGCCCC TGCCTCTCGG GCTGCGCCTG TCCCCAGGGT 
CTGCTCAGAC ACGGGGATGC ATGTTTCCTG CCAGAGGAGT GCCCCTGCAC TTGGAAGGGG 
AAGGAGTATT TCCCTGGGGA CCAGGTGATG TCTCCTTGCC ATACCTGTGT GTGCCAGCGG 
GGCTCATTCC AGTGCACCCT GCACCCTTGC GCCTCCACCT GCACTGCCTA TGGGGACCGG 
CATTACCGCA CGTTTGATGG GCTCCCGTTT GACTTCGTGG GGGCATGCAA AGTGCACCTG 
GTCAAGAGCA CATCAGATGT CAGCTTCTCT GTGATTGTAG AGAATGTGAA CTGCTACAGC 
TCTGGCATGA TCTGCAGGAA ATTTATTTCC ATCAACGTTG GGAACTCACT CATTGTCTTT 
GATGATGACT CCGGAAATCC TAGTCCAGAG AGCTTCCTGG ATGACAAGCA GGAGGTCCAC 
ACATGGCGAG TGGGATTTTT CACACTGGTG CATTTCCCAC AGGAGCACAT CACCCTCTTG 
TGGGACCAGA GAACCACAGT GCACGTCCAG GCTGGGCCTC AGTGGCAGGG CCAGCTGGCG 
GGCCTCTGTG GGAACTTTGA CTTAAAAACC ATCAATGAGA TGAGGACCCC GGAGAACCTA 
GAGCTAACTA ACCCCCAGGA GTTTGGCAGC AGTTGGGCTG CAGTTGAGTG CCCAGACACC 
CTCGATCCTC GGGATATGTG TGTCCTGAAT CCTCTCCGAG AACCATTTGC CAAGAAGGAG 
TGCAGCATCC TGCTCAGTGA GGTGTTTGAG ATCTGCCACC CTGTGGTTGA TGTCACTTGG 
TTTTACTCAA ACTGCCTGAC AGACACATGT GGCTGCAGCC AGGGTGGTGA CTGTGAGTGC 
TTCTGTGCCA GCGTCTCCGC TTATGCCCAC CAGTGTTGCC AGCATGGGGT GGCTGTTGAC 
TGGCGAACCC CCCGCCTCTG CCCGTATGAC TGTGACTTCT TTAACAAAGT GCTAGGTAAG 
GGCCCCTATC AGCTATCCAG CTTGGCAGCC GGTGGTGCTC TGGTGGGCAT GAAGGCGGTG 
GGCGATGACA TAGTCCTAGT GAGGACAGAG GATGTGGCGC CAGCAGACAT TGTGAGCTTC 
CTGCTGACAG CTGCTCTGTA CAAGGCCAAG GCCCATGACC CAGATGTGGT GTCCCTGGAG 
GCAGCAGACA GACCCAACTT CTTCCTTCAC GTCACAGCCA ACGGGTCTCT GGAGCTGGCT 
AAGTGGCAGG GCCGTGACAC CTTCCAACAG CATGCCTCCT TCTTGCTGCA CCGGGGGACA 
CGGCAGGCAG GCCTGGTGGC CCTGGAGTCC CTGGCCAAGC CCAGCTCCTT CCTCTATGTG 
TCGGGCGCGG TGCTGGCCCT GCGGCTGTAC GAACACACAG AGGTGTTCCG CCGGGGCACA 
CTCTTCCGCC TTCTGGATGC CAAGCCCTCG GGGGCTGCCT ACCCCATCTG CGAGTGGCGC 
TACGATGCCT GTGCCAGCCC CTGCTTCCAA ACCTGCCGGG ACCCACGGGC AGCCAGCTGC 
CGGGACGTAC CCAGGGTAGA AGGCTGTGTC CCTGTGTGCC CCACCCCCCA GGTCCTGGAT 
GAAGTCACAC AGAGATGTGT CTACTTGGAG GACTGTGTGG AGCCAGCAGT TTGGGTTCCC 
ACAGAGGCCC TTGGCAATGA GACCCTCCCT CCCAGTCAAG GGTTGCCCAC TCCCAGTGAT 
GAGGAGCCAC AGCTGTCACA GGAAAGCCCC AGGACCCCCA CCCACAGGCC AGCCCTCACC 
CCAGCTGCCC CACTCACCAC AGCCCTGAAC CCACCAGTGA CAGCCACTGA GGAGCCAGTG 
GTGTCTCCAG GCCCCACCCA GACCACCCTG CAGCAGCCAC TGGAGCTCAC TGCATCTCAA 
CTCCCCGCCG GCCCCACGGA GTCCCCAGCC AGCAAGGGAG TGACTGCCAG CCTCCTGGCC 
ATCCCCCATA CACCAGAGTC CTCATCCCTC CCTGTTGCAC TGCAGACACC CACACCTGGC 
ATGGTGTCAG GTGCCATGGA GACAACAAGG GTGACTGTGA TCTTTGCAGG AAGCCCTAAC 
ATCACAGTCT CCTCCCGGTC GCCCCCTGCC CCTCGCTTCC CGCTCATGAC CAAGGCTGTG 
ACAGTCCGAG GCCATGGCTC CTTGCCTGTT AGGACGACAC CCCCACAGCC CTCCTTGACA 
GCAAGTCCCT CCTCCAGACC TGTGGCTTCC CCTGGAGCCA TCTCCAGGTC CCCCACCTCC 
TCGGGATCCC ACAAGGCTGT GCTGACACCT GCAGTAACTA AGGTCATAAG CAGGACAGGG 
GTCCCCCAGC CCACCCAGGC CCAGAGTGCT TCAAGTCCCA GCACCCCTCT AACTGTGGCT 
GGAACAGCAG CAGAACAGGT TCCTGTCAGT CCCCTTGCAA CCAGGAGCTT GGAGATAGTG 
CTATCCACAG AGAAGGGCGA AGCCGGGCAC AGCCAGCCCA TGGGCTCGCC TGCCTCCCCA 
CAGCCACACC CACTCCCCTC TGCACCACCC CGCCCAGCCC AGCATACCAC CATGGCCACC 
AGGTCTCCAG CTCTGCCCCC AGAGACCCCA GCTGCCGCCA GCCTGTCAAC AGCCACTGAT 
GGGCTGGCAG CCACACCCTT CATGTCCCTT GAGTCAACTC GTCCCTCCCA GCTCCTCTCT 
GGCCTGCCTC CCGACACCAG CCTGCCCCTG GCCAAGGTGG GCACATCTGC CCCAGTGGCC 
ACACCCGGCC CCAAAGCCTC TGTCATCACC ACTCCACTCC AGCCACAGGC CACGACTCTG 
CCTGCTCAGA CACTTAGCCC AGTACTGCCT TTCACTCCAG CAGCAATGAC CCAGGCGCAC 
CCACCCACTC ACATAGCACC CCCAGCAGCA GGCACAGCTC CAGGCCTGCT GCTGGGAGCC 
ACATTGCCAA CCTCTGGAGT CCTGCCTGTG GCTGAGGGCA CGGCCTCCAT GGTATCTGTT 
GTCCCACGAA AGAGCACCAC AGGGAAGGTG GCCATCCTAT CCAAGCAAGT GTCTCTGCCC 
ACTTCCATGT ATGGTTCTGC AGAGGGTGGG CCCACAGAGC TCACGCCTGC TACGAGCCAC 
CCTCTCACGC CCTTGGTGGC TGAGCCCGAG GGAGCCCAGG CAGGCACAGC TCTGCCAGTG 
CCCACATCCT ATGCCCTGAG CCGTGTCTCA GCCAGGACGG CCCCCCAAGA CAGCATGCTG 
GTTCTGTTGC CTCAGCTGGC TGAGGCCCAT GGAACCTCGG CAGGGCCTCA CCTGGCAGCA 
GAGCCGGTGG ACGAGGCCAC CACAGAACCA TCTGGGCGCT CAGCCCCAGC CCTGAGCATC 
GTAGAGGGTT TGGCGGAGGC TTTGGCAACT ACCACTGAGG CCAATACATC CACCACCTGT 
GTTCCAATCG CCGAGCAGGA CTGCGTCCGC CACATCTGCC TGGAGGGCCA GCTGATTCGC 
GTGAATCAGT CCCAGCACTG TCCCCAGGGT GCTGCTCCCC CTCGCTGTGG GATCCTGGGC 
CTCGCCGTGC GGGTGGGTGG GGACCGCTGC TGCCCACTCT GGGAGTGTGC CTGCCGGTGC 
TCAATCTTCC CTGACCTGAG CTTCGTGACC TTCGATGGGA GCCACGTAGC TCTGTTCAAG 
GAGGCCATCT ACATCCTCAG CCAGAGCCCA GATGAAATGC TCACCGTCCA TGTACTGGAC 
TGCAAAAGTG CCAACCTGGG GCACCTGAAC TGGCCCCCGT TCTGTCTGGT GATGTTGAAC 
ATGACTCACT TGGCCCATCA GGTCACTATT GATCGCTTCA ACCGAAAGGT GACTGTGGAC 
TTGCAGCCTG TGTGGCCACC GGTGAGCAGG TATGGATTCA GAATTGAGGA CACAGGCCAC 
ATGTACATGA TCCTGACTCC CTCAGACATC CAGATCCAGT GGCTCCACAG CTCAGGACTC 
ATGATCGTGG AGGCCAGCAA AACCAGCAAG GCCCAGGGCC ATGGCCTGTG CGGTATCTGT 
GATGGAGATG CAGCCAATGA CCTTACCCTG AAGGATGGCT CAGTGGTGGG TGGGGCTGAG 
GACCCTGCTC CCTTTCTGGA CAGCTGGCAG GTGCCCAGCT CCCTGACCTC AGTGGGCCAG 
ACCCGCTTCC GCCCAGACAG CTGCGCCACA ACTGACTGCT CGCCCTGCCT TCGCATGGTG 
TCCAACCGCA CCTTCAGTGC CTGCCACCGC TTTGTGCCTC CGGAGTCATT CTGTGAGCTG 
TGGATCCGGG ACACCAAGTA CGTGCAGCAG CCCTGCGTGG CCCTGACTGT GTACGTGGCC 
ATGTGCCACA AATTTCATGT GTGCATCGAG TGGCGGCGCT CTGACTACTG CCCCTTCCTG 
TGCTCCAGCG ACTCCACATA CCAGGCATGT GTGACAGCCT GTGAGCCACC CAAGACATGC 
CAGGATGGGA TACTAGGGCC TCTGGACCCA GAGCACTGCC AGGTGCTGGG CGAGGGCTGC 
GTCTGCTCCG AGGGCACCAT CTTACACCGG CGCCACTCTG CACTCTGCAT CCCGGAGGCC 
AAGTGCGCCT GCACTGACAG CATGGGGGTG CCGAGGGCCC TGGGGGAGAC CTGGAACAGC 
TCCCTCAGCG GCTGCTGCCA GCACCAGTGC CAAGCCCCAG ACACCATTGT CCCGGTGGAT 
CTGGGCTGCC CCAGTCCCCG CCCTGAGAGC TGCCTGCGAT TCGGGGAGGT GGCCTTGCTC 
CTACCCACCA AGGACCCCTG CTGCCTGGGG ACTGTCTGTG TGTGTAACCA GACTCTGTGT 
GAGGGTCTCG CCCCCACATG CCGCCCAGGC CACCGCCTCC TCACCCACTT CCAGGAGGAC 
TCCTGCTGCC CCAGCTACAG CTGTGAGTGT GACCCAGATC TCTGTGAGGC AGAGCTGGTC 
CCCAGCTGCC GACAGGACCA GATCCTGATC ACGGGCCGCC TGGGGGACTC CTGCTGCACC 
TCCTACTTCT GCGCCTGTGG TGACTGTCCA GACTCCATCC CCGAATGTCA AGAAGGGGAG 
GCGCTCACTG TGCACAGGAA TACCACGGAA CTCTGCTGCC CTCTGTACCA GTGTGTGTGT 
GAGAACTTCC GCTGTCCCCA AGTGCAGTGT GGCCTGGGCA CTGCCCTGGT GGAGGTGTGG 
AGCCCCGACC GCTGCTGCCC CTACAAATCC TGTGAATGTG ACTGTGACAC AATCCCGGTG 
CCCCGGTGCC ATCTGTGGGA GAAATCCCAG CTGGATGAGG AGTTCATGCA CAGCGTGGAG 
AATGTGTGTG GCTGCGCCAA GTACGAGTGT GTGAAGGCCC CGGTGTGTCT GAGCCGCGAG 
CTGGGTGTGA TGCAGCCCGG CCAGACAGTG GTGGAGCTCT CAGCAGATGG CGTGTGCCAC 
ACCTCCCGCT GCACCACCGT GCTCGACCCT CTCACCAACT TCTACCAGAT CAACACCACC 
TCCGTGCTCT GTGACATCCA CTGTGAGGCG AACCAGGAGT ACGAGCACCC GCGGGACCTC 
GCTGCCTGCT GCGGCTCCTG CAGGAACGTG TCCTGTCTCT TCACCTTCCC CAATGGCACC 
ACCTCCCTGT TCTTGCCCGG GGCATCCTGG ATCGCAGACT GCGCCCGCCA CCACTGCAGC 
AGCACGCCCC TGGGTGCCGT GCTGGTCCGC TCTCCCATAA GCTGCCCACC GCTCAATGAG 
ACTGAGTGTG CCAAGGTTGG GGGTTCCGTG GTACCTTCCT TGGAAGGATG CTGCAGGACC 
TGTAAGGAGG ATGGGCGCTC CTGCAAGAAG GTGACCATCC GCATGACCAT CCGCAAGAAT 
GAATGCAGGA GCAGCACCCC TGTGAACCTA GTGTCCTGCG ATGGGAGGTG CCCATCCGCC 
AGCATCTACA ACTACAACAT CAACACCTAT GCCCGATTCT GCAAGTGCTG CCGTGAGGTG 
GGCCTGCAGC GGCGCTCTGT GCAGCTCTTC TGTGCCACCA ATGCCACCTG GGTGCCCTAT 
ACAGTGCAGG AGCCCACCGA CTGTGCCTGC CAGTGGTCCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOG ( NM_001277269.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264198.1 Homo sapiens otogelin (OTOG), transcript variant 1, mRNA.

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CloneID	OHu27814
Clone ID Related Accession (Same CDS sequence)	NM_001277269.2 , NM_001277269.1
Accession Version	NM_001277269.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	otogelin isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124799.5 and KC877393.1. On Sep 3, 2019 this sequence version replaced NM_001277269.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. CCDS Note: This CCDS representation lacks full-length human transcript support. Its exon combination is therefore inferred, but it is supported by partial human transcript alignments and data in PMID:23122587. ##RefSeq-Attributes-START## CDS uses downstream in-frame AUG :: upstream AUG and CDS extension is not conserved inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGGTGAGA GGGTTGTGGA CTCAGGGAGG 
TCGGGGGCTC GAGGAATGAG AAACGTTAAA GGAATGAGGA ATGGGCCCGC GCAGACTCGT 
GTTTCCTCCA GCAGCAGCCA CCAGGAGGCG ACCCTTGCCA TGGGGGACAA GGCTACAGTC 
GTGGGAGGCC AGCAGGCTGA AGCCCCAGAC TCCGTGGCCA TGTCTTCCTG GGAAAGGCGG 
CTCCATCGGG CCAAGTGTGC ACCATCCTAC TTGTTCTCCT GCTTCAATGG AGGCGAGTGT 
GTGCACCCAG CCTTCTGTGA CTGCAGACGC TTCAATGCCA CTGGACCGCG CTGCCAGATG 
GTGTACAATG CCGGCCCTGA GAGGGACAGC ATTTGCCGGG CGTGGGGGCA GCACCACGTG 
GAGACATTTG ATGGGCTCTA CTACTACCTC TCCGGAAAGG GCAGCTACAC CCTGGTGGGT 
CGCCATGAGC CCGAGGGACA GAGCTTCTCC ATCCAGGTAC ACAATGACCC GCAGTGTGGC 
TCTTCACCCT ACACCTGCTC CAGGGCTGTC AGCCTCTTCT TTGTGGGTGA GCAGGAGATC 
CATCTGGCCA AGGAGGTCAC CCATGGAGGC ATGAGGGTCC AACTGCCACA TGTCATGGGG 
AGCGCGCGTC TGCAGCAGCT TGCCGGCTAT GTCATCGTGC GGCATCAGTC AGCCTTCACA 
CTGGCCTGGG ATGGTGCCTC GGCTGTCTAC ATCAAGATGA GTCCAGAGCT TCTGGGCTGG 
ACCCATGGGC TGTGTGGGAA CAACAATGCT GACCCCAAGG ATGATCTGGT GACCAGCTCT 
GGGAAGCTGA CTGACGACGT GGTTGAGTTT GTGCACAGCT GGCAGGAGCA GGCCCCTAAC 
CAGCCTCCAG GGCCCACAAC TTCCTCCCTG CCTCGCCCAC CGTGCCTACA GCAGAACCCA 
GGAACCATGC AGGGCGTGTA CGAGCAGTGT GAGGCTCTAC TGCGGCCCCC CTTTGACGCC 
TGCCACGCCT ACGTCAGCCC TCTGCCCTTC ACAGCCAGTT GTACCAGTGA TCTCTGCCAA 
TCAATGGGTG ATGTAGCCAC CTGGTGCCGG GCACTGGCGG AGTATGCCCG GGCGTGTGCC 
CAGGCAGGGC GGCCCTTGCA AGGCTGGAGG ACCCAGCTCC GGCAATGCAC TGTGCACTGC 
AAGGAGAAGG CCTTTACCTA CAATGAGTGC ATCGCCTGCT GCCCTGCCTC CTGCCATCCC 
CGGGCATCCT GTGTGGACAG TGAGATCGCC TGTGTGGACG GCTGCTATTG CCCCAATGGG 
CTCATCTTCG AGGATGGGGG CTGCGTGGCA CCAGCTGAGT GTCCCTGTGA GTTTCACGGG 
ACTCTGTACC CACCTGGCTC TGTGGTGAAG GAAGACTGCA ATACTTGCAC ATGCACCTCA 
GGCAAGTGGG AGTGCAGCAC AGCTGTCTGC CCAGCTGAGT GCTCAGTGAC TGGTGACATT 
CACTTCACAA CCTTTGATGG CCGCCGGTAC ACGTTCCCCG CCACATGTCA GTACATCCTG 
GCCAAGAGCC GCTCTTCGGG CACCTTCACC GTGACATTGC AGAATGCCCC ATGTGGCCTG 
AACCAAGATG GAGCCTGTGT CCAGTCAGTG TCAGTGATTC TGCACCAGGA CCCTCGGAGG 
CAGGTGACCC TGACCCAGGC AGGGGATGTC CTTCTGTTTG ACCAGTACAA GATCATCCCG 
CCATACACAG ATGATGCCTT TGAGATCCGT AGGCTGTCCT CCGTGTTCCT GCGGGTGAGG 
ACGAACGTGG GCGTGCGGGT GCTCTACGAC CGTGAAGGGC TCCGACTGTA CCTGCAAGTG 
GACCAGCGAT GGGTGGAGGA TACCGTGGGC CTCTGCGGCA CCTTCAATGG CAACACGCAG 
GATGACTTCC TGTCTCCAGT GGGTGTACCT GAGAGCACCC CACAACTTTT TGGCAATTCC 
TGGAAAACAC TTTCTGCTTG CTCCCCGCTG GTCTCTGGCT CCCCTCTGGA CCCCTGCGAT 
GTGCACCTGC AAGCCGCCTC CTACTCAGTG CAGGCCTGCA GCGTGCTCAC GGGGGAGATG 
TTTGCGCCCT GCTCTGCGTT CCTGAGCCCC GTGCCCTACT TTGAGCAGTG CCGCAGGGAT 
GCCTGCCGCT GCGGGCAGCC CTGCCTGTGC GCCACACTGG CCCACTACGC CCACCTGTGC 
CGGCGCCATG GGCTCCCCGT TGATTTCCGC GCCCGCCTGC CAGCCTGTGC ACTGTCCTGT 
GAGGCCTCCA AGGAGTATAG CCCCTGCGTG GCCCCGTGTG GACGTACCTG CCAGGACCTG 
GCCAGCCCTG AGGCCTGTGG GGTTGATGGT GGCGATGACC TGAGCAGAGA CGAGTGTGTG 
GAGGGCTGTG CCTGCCCACC GGACACCTAT CTGGACACCC AGGCTGACCT CTGTGTCCCC 
CGGAACCAGT GCTCCTGCCA CTTCCAGGGA GTGGACTATC CCCCCGGAGA CAGTGACATC 
CCATCCCTGG GCCACTGCCA CTGCAAAGAT GGAGTCATGA GCTGTGATAG CAGAGCCCCA 
GCTGCTGCCT GCCCAGCAGG CCAGGTCTTC GTGAACTGCA GCGACCTGCA CACGGACCTG 
GAGCTGAGCA GGGAGAGGAC GTGTGAGCAG CAACTGCTGA ACCTGAGCGT GTCAGCCCGT 
GGCCCCTGCC TCTCGGGCTG CGCCTGTCCC CAGGGTCTGC TCAGACACGG GGATGCATGT 
TTCCTGCCAG AGGAGTGCCC CTGCACTTGG AAGGGGAAGG AGTATTTCCC TGGGGACCAG 
GTGATGTCTC CTTGCCATAC CTGTGTGTGC CAGCGGGGCT CATTCCAGTG CACCCTGCAC 
CCTTGCGCCT CCACCTGCAC TGCCTATGGG GACCGGCATT ACCGCACGTT TGATGGGCTC 
CCGTTTGACT TCGTGGGGGC ATGCAAAGTG CACCTGGTCA AGAGCACATC AGATGTCAGC 
TTCTCTGTGA TTGTAGAGAA TGTGAACTGC TACAGCTCTG GCATGATCTG CAGGAAATTT 
ATTTCCATCA ACGTTGGGAA CTCACTCATT GTCTTTGATG ATGACTCCGG AAATCCTAGT 
CCAGAGAGCT TCCTGGATGA CAAGCAGGAG GTCCACACAT GGCGAGTGGG ATTTTTCACA 
CTGGTGCATT TCCCACAGGA GCACATCACC CTCTTGTGGG ACCAGAGAAC CACAGTGCAC 
GTCCAGGCTG GGCCTCAGTG GCAGGGCCAG CTGGCGGGCC TCTGTGGGAA CTTTGACTTA 
AAAACCATCA ATGAGATGAG GACCCCGGAG AACCTAGAGC TAACTAACCC CCAGGAGTTT 
GGCAGCAGTT GGGCTGCAGT TGAGTGCCCA GACACCCTCG ATCCTCGGGA TATGTGTGTC 
CTGAATCCTC TCCGAGAACC ATTTGCCAAG AAGGAGTGCA GCATCCTGCT CAGTGAGGTG 
TTTGAGATCT GCCACCCTGT GGTTGATGTC ACTTGGTTTT ACTCAAACTG CCTGACAGAC 
ACATGTGGCT GCAGCCAGGG TGGTGACTGT GAGTGCTTCT GTGCCAGCGT CTCCGCTTAT 
GCCCACCAGT GTTGCCAGCA TGGGGTGGCT GTTGACTGGC GAACCCCCCG CCTCTGCCCG 
TATGACTGTG ACTTCTTTAA CAAAGTGCTA GGTAAGGGCC CCTATCAGCT ATCCAGCTTG 
GCAGCCGGTG GTGCTCTGGT GGGCATGAAG GCGGTGGGCG ATGACATAGT CCTAGTGAGG 
ACAGAGGATG TGGCGCCAGC AGACATTGTG AGCTTCCTGC TGACAGCTGC TCTGTACAAG 
GCCAAGGCCC ATGACCCAGA TGTGGTGTCC CTGGAGGCAG CAGACAGACC CAACTTCTTC 
CTTCACGTCA CAGCCAACGG GTCTCTGGAG CTGGCTAAGT GGCAGGGCCG TGACACCTTC 
CAACAGCATG CCTCCTTCTT GCTGCACCGG GGGACACGGC AGGCAGGCCT GGTGGCCCTG 
GAGTCCCTGG CCAAGCCCAG CTCCTTCCTC TATGTGTCGG GCGCGGTGCT GGCCCTGCGG 
CTGTACGAAC ACACAGAGGT GTTCCGCCGG GGCACACTCT TCCGCCTTCT GGATGCCAAG 
CCCTCGGGGG CTGCCTACCC CATCTGCGAG TGGCGCTACG ATGCCTGTGC CAGCCCCTGC 
TTCCAAACCT GCCGGGACCC ACGGGCAGCC AGCTGCCGGG ACGTACCCAG GGTAGAAGGC 
TGTGTCCCTG TGTGCCCCAC CCCCCAGGTC CTGGATGAAG TCACACAGAG ATGTGTCTAC 
TTGGAGGACT GTGTGGAGCC AGCAGTTTGG GTTCCCACAG AGGCCCTTGG CAATGAGACC 
CTCCCTCCCA GTCAAGGGTT GCCCACTCCC AGTGATGAGG AGCCACAGCT GTCACAGGAA 
AGCCCCAGGA CCCCCACCCA CAGGCCAGCC CTCACCCCAG CTGCCCCACT CACCACAGCC 
CTGAACCCAC CAGTGACAGC CACTGAGGAG CCAGTGGTGT CTCCAGGCCC CACCCAGACC 
ACCCTGCAGC AGCCACTGGA GCTCACTGCA TCTCAACTCC CCGCCGGCCC CACGGAGTCC 
CCAGCCAGCA AGGGAGTGAC TGCCAGCCTC CTGGCCATCC CCCATACACC AGAGTCCTCA 
TCCCTCCCTG TTGCACTGCA GACACCCACA CCTGGCATGG TGTCAGGTGC CATGGAGACA 
ACAAGGGTGA CTGTGATCTT TGCAGGAAGC CCTAACATCA CAGTCTCCTC CCGGTCGCCC 
CCTGCCCCTC GCTTCCCGCT CATGACCAAG GCTGTGACAG TCCGAGGCCA TGGCTCCTTG 
CCTGTTAGGA CGACACCCCC ACAGCCCTCC TTGACAGCAA GTCCCTCCTC CAGACCTGTG 
GCTTCCCCTG GAGCCATCTC CAGGTCCCCC ACCTCCTCGG GATCCCACAA GGCTGTGCTG 
ACACCTGCAG TAACTAAGGT CATAAGCAGG ACAGGGGTCC CCCAGCCCAC CCAGGCCCAG 
AGTGCTTCAA GTCCCAGCAC CCCTCTAACT GTGGCTGGAA CAGCAGCAGA ACAGGTTCCT 
GTCAGTCCCC TTGCAACCAG GAGCTTGGAG ATAGTGCTAT CCACAGAGAA GGGCGAAGCC 
GGGCACAGCC AGCCCATGGG CTCGCCTGCC TCCCCACAGC CACACCCACT CCCCTCTGCA 
CCACCCCGCC CAGCCCAGCA TACCACCATG GCCACCAGGT CTCCAGCTCT GCCCCCAGAG 
ACCCCAGCTG CCGCCAGCCT GTCAACAGCC ACTGATGGGC TGGCAGCCAC ACCCTTCATG 
TCCCTTGAGT CAACTCGTCC CTCCCAGCTC CTCTCTGGCC TGCCTCCCGA CACCAGCCTG 
CCCCTGGCCA AGGTGGGCAC ATCTGCCCCA GTGGCCACAC CCGGCCCCAA AGCCTCTGTC 
ATCACCACTC CACTCCAGCC ACAGGCCACG ACTCTGCCTG CTCAGACACT TAGCCCAGTA 
CTGCCTTTCA CTCCAGCAGC AATGACCCAG GCGCACCCAC CCACTCACAT AGCACCCCCA 
GCAGCAGGCA CAGCTCCAGG CCTGCTGCTG GGAGCCACAT TGCCAACCTC TGGAGTCCTG 
CCTGTGGCTG AGGGCACGGC CTCCATGGTA TCTGTTGTCC CACGAAAGAG CACCACAGGG 
AAGGTGGCCA TCCTATCCAA GCAAGTGTCT CTGCCCACTT CCATGTATGG TTCTGCAGAG 
GGTGGGCCCA CAGAGCTCAC GCCTGCTACG AGCCACCCTC TCACGCCCTT GGTGGCTGAG 
CCCGAGGGAG CCCAGGCAGG CACAGCTCTG CCAGTGCCCA CATCCTATGC CCTGAGCCGT 
GTCTCAGCCA GGACGGCCCC CCAAGACAGC ATGCTGGTTC TGTTGCCTCA GCTGGCTGAG 
GCCCATGGAA CCTCGGCAGG GCCTCACCTG GCAGCAGAGC CGGTGGACGA GGCCACCACA 
GAACCATCTG GGCGCTCAGC CCCAGCCCTG AGCATCGTAG AGGGTTTGGC GGAGGCTTTG 
GCAACTACCA CTGAGGCCAA TACATCCACC ACCTGTGTTC CAATCGCCGA GCAGGACTGC 
GTCCGCCACA TCTGCCTGGA GGGCCAGCTG ATTCGCGTGA ATCAGTCCCA GCACTGTCCC 
CAGGGTGCTG CTCCCCCTCG CTGTGGGATC CTGGGCCTCG CCGTGCGGGT GGGTGGGGAC 
CGCTGCTGCC CACTCTGGGA GTGTGCCTGC CGGTGCTCAA TCTTCCCTGA CCTGAGCTTC 
GTGACCTTCG ATGGGAGCCA CGTAGCTCTG TTCAAGGAGG CCATCTACAT CCTCAGCCAG 
AGCCCAGATG AAATGCTCAC CGTCCATGTA CTGGACTGCA AAAGTGCCAA CCTGGGGCAC 
CTGAACTGGC CCCCGTTCTG TCTGGTGATG TTGAACATGA CTCACTTGGC CCATCAGGTC 
ACTATTGATC GCTTCAACCG AAAGGTGACT GTGGACTTGC AGCCTGTGTG GCCACCGGTG 
AGCAGGTATG GATTCAGAAT TGAGGACACA GGCCACATGT ACATGATCCT GACTCCCTCA 
GACATCCAGA TCCAGTGGCT CCACAGCTCA GGACTCATGA TCGTGGAGGC CAGCAAAACC 
AGCAAGGCCC AGGGCCATGG CCTGTGCGGT ATCTGTGATG GAGATGCAGC CAATGACCTT 
ACCCTGAAGG ATGGCTCAGT GGTGGGTGGG GCTGAGGACC CTGCTCCCTT TCTGGACAGC 
TGGCAGGTGC CCAGCTCCCT GACCTCAGTG GGCCAGACCC GCTTCCGCCC AGACAGCTGC 
GCCACAACTG ACTGCTCGCC CTGCCTTCGC ATGGTGTCCA ACCGCACCTT CAGTGCCTGC 
CACCGCTTTG TGCCTCCGGA GTCATTCTGT GAGCTGTGGA TCCGGGACAC CAAGTACGTG 
CAGCAGCCCT GCGTGGCCCT GACTGTGTAC GTGGCCATGT GCCACAAATT TCATGTGTGC 
ATCGAGTGGC GGCGCTCTGA CTACTGCCCC TTCCTGTGCT CCAGCGACTC CACATACCAG 
GCATGTGTGA CAGCCTGTGA GCCACCCAAG ACATGCCAGG ATGGGATACT AGGGCCTCTG 
GACCCAGAGC ACTGCCAGGT GCTGGGCGAG GGCTGCGTCT GCTCCGAGGG CACCATCTTA 
CACCGGCGCC ACTCTGCACT CTGCATCCCG GAGGCCAAGT GCGCCTGCAC TGACAGCATG 
GGGGTGCCGA GGGCCCTGGG GGAGACCTGG AACAGCTCCC TCAGCGGCTG CTGCCAGCAC 
CAGTGCCAAG CCCCAGACAC CATTGTCCCG GTGGATCTGG GCTGCCCCAG TCCCCGCCCT 
GAGAGCTGCC TGCGATTCGG GGAGGTGGCC TTGCTCCTAC CCACCAAGGA CCCCTGCTGC 
CTGGGGACTG TCTGTGTGTG TAACCAGACT CTGTGTGAGG GTCTCGCCCC CACATGCCGC 
CCAGGCCACC GCCTCCTCAC CCACTTCCAG GAGGACTCCT GCTGCCCCAG CTACAGCTGT 
GAGTGTGACC CAGATCTCTG TGAGGCAGAG CTGGTCCCCA GCTGCCGACA GGACCAGATC 
CTGATCACGG GCCGCCTGGG GGACTCCTGC TGCACCTCCT ACTTCTGCGC CTGTGGTGAC 
TGTCCAGACT CCATCCCCGA ATGTCAAGAA GGGGAGGCGC TCACTGTGCA CAGGAATACC 
ACGGAACTCT GCTGCCCTCT GTACCAGTGT GTGTGTGAGA ACTTCCGCTG TCCCCAAGTG 
CAGTGTGGCC TGGGCACTGC CCTGGTGGAG GTGTGGAGCC CCGACCGCTG CTGCCCCTAC 
AAATCCTGTG AATGTGACTG TGACACAATC CCGGTGCCCC GGTGCCATCT GTGGGAGAAA 
TCCCAGCTGG ATGAGGAGTT CATGCACAGC GTGGAGAATG TGTGTGGCTG CGCCAAGTAC 
GAGTGTGTGA AGGCCCCGGT GTGTCTGAGC CGCGAGCTGG GTGTGATGCA GCCCGGCCAG 
ACAGTGGTGG AGCTCTCAGC AGATGGCGTG TGCCACACCT CCCGCTGCAC CACCGTGCTC 
GACCCTCTCA CCAACTTCTA CCAGATCAAC ACCACCTCCG TGCTCTGTGA CATCCACTGT 
GAGGCGAACC AGGAGTACGA GCACCCGCGG GACCTCGCTG CCTGCTGCGG CTCCTGCAGG 
AACGTGTCCT GTCTCTTCAC CTTCCCCAAT GGCACCACCT CCCTGTTCTT GCCCGGGGCA 
TCCTGGATCG CAGACTGCGC CCGCCACCAC TGCAGCAGCA CGCCCCTGGG TGCCGTGCTG 
GTCCGCTCTC CCATAAGCTG CCCACCGCTC AATGAGACTG AGTGTGCCAA GGTTGGGGGT 
TCCGTGGTAC CTTCCTTGGA AGGATGCTGC AGGACCTGTA AGGAGGATGG GCGCTCCTGC 
AAGAAGGTGA CCATCCGCAT GACCATCCGC AAGAATGAAT GCAGGAGCAG CACCCCTGTG 
AACCTAGTGT CCTGCGATGG GAGGTGCCCA TCCGCCAGCA TCTACAACTA CAACATCAAC 
ACCTATGCCC GATTCTGCAA GTGCTGCCGT GAGGTGGGCC TGCAGCGGCG CTCTGTGCAG 
CTCTTCTGTG CCACCAATGC CACCTGGGTG CCCTATACAG TGCAGGAGCC CACCGACTGT 
GCCTGCCAGT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264198.1
CDS	115..8892
Translation	MGVLASALCWLLCVWLPWGEQAAESLRVQRLGERVVDSGRSGARGMRNVKGMRNGPAQTRVSSSSSHQEATLAMGDKATVVGGQQAEAPDSVAMSSWERRLHRAKCAPSYLFSCFNGGECVHPAFCDCRRFNATGPRCQMVYNAGPERDSICRAWGQHHVETFDGLYYYLSGKGSYTLVGRHEPEGQSFSIQVHNDPQCGSSPYTCSRAVSLFFVGEQEIHLAKEVTHGGMRVQLPHVMGSARLQQLAGYVIVRHQSAFTLAWDGASAVYIKMSPELLGWTHGLCGNNNADPKDDLVTSSGKLTDDVVEFVHSWQEQAPNQPPGPTTSSLPRPPCLQQNPGTMQGVYEQCEALLRPPFDACHAYVSPLPFTASCTSDLCQSMGDVATWCRALAEYARACAQAGRPLQGWRTQLRQCTVHCKEKAFTYNECIACCPASCHPRASCVDSEIACVDGCYCPNGLIFEDGGCVAPAECPCEFHGTLYPPGSVVKEDCNTCTCTSGKWECSTAVCPAECSVTGDIHFTTFDGRRYTFPATCQYILAKSRSSGTFTVTLQNAPCGLNQDGACVQSVSVILHQDPRRQVTLTQAGDVLLFDQYKIIPPYTDDAFEIRRLSSVFLRVRTNVGVRVLYDREGLRLYLQVDQRWVEDTVGLCGTFNGNTQDDFLSPVGVPESTPQLFGNSWKTLSACSPLVSGSPLDPCDVHLQAASYSVQACSVLTGEMFAPCSAFLSPVPYFEQCRRDACRCGQPCLCATLAHYAHLCRRHGLPVDFRARLPACALSCEASKEYSPCVAPCGRTCQDLASPEACGVDGGDDLSRDECVEGCACPPDTYLDTQADLCVPRNQCSCHFQGVDYPPGDSDIPSLGHCHCKDGVMSCDSRAPAAACPAGQVFVNCSDLHTDLELSRERTCEQQLLNLSVSARGPCLSGCACPQGLLRHGDACFLPEECPCTWKGKEYFPGDQVMSPCHTCVCQRGSFQCTLHPCASTCTAYGDRHYRTFDGLPFDFVGACKVHLVKSTSDVSFSVIVENVNCYSSGMICRKFISINVGNSLIVFDDDSGNPSPESFLDDKQEVHTWRVGFFTLVHFPQEHITLLWDQRTTVHVQAGPQWQGQLAGLCGNFDLKTINEMRTPENLELTNPQEFGSSWAAVECPDTLDPRDMCVLNPLREPFAKKECSILLSEVFEICHPVVDVTWFYSNCLTDTCGCSQGGDCECFCASVSAYAHQCCQHGVAVDWRTPRLCPYDCDFFNKVLGKGPYQLSSLAAGGALVGMKAVGDDIVLVRTEDVAPADIVSFLLTAALYKAKAHDPDVVSLEAADRPNFFLHVTANGSLELAKWQGRDTFQQHASFLLHRGTRQAGLVALESLAKPSSFLYVSGAVLALRLYEHTEVFRRGTLFRLLDAKPSGAAYPICEWRYDACASPCFQTCRDPRAASCRDVPRVEGCVPVCPTPQVLDEVTQRCVYLEDCVEPAVWVPTEALGNETLPPSQGLPTPSDEEPQLSQESPRTPTHRPALTPAAPLTTALNPPVTATEEPVVSPGPTQTTLQQPLELTASQLPAGPTESPASKGVTASLLAIPHTPESSSLPVALQTPTPGMVSGAMETTRVTVIFAGSPNITVSSRSPPAPRFPLMTKAVTVRGHGSLPVRTTPPQPSLTASPSSRPVASPGAISRSPTSSGSHKAVLTPAVTKVISRTGVPQPTQAQSASSPSTPLTVAGTAAEQVPVSPLATRSLEIVLSTEKGEAGHSQPMGSPASPQPHPLPSAPPRPAQHTTMATRSPALPPETPAAASLSTATDGLAATPFMSLESTRPSQLLSGLPPDTSLPLAKVGTSAPVATPGPKASVITTPLQPQATTLPAQTLSPVLPFTPAAMTQAHPPTHIAPPAAGTAPGLLLGATLPTSGVLPVAEGTASMVSVVPRKSTTGKVAILSKQVSLPTSMYGSAEGGPTELTPATSHPLTPLVAEPEGAQAGTALPVPTSYALSRVSARTAPQDSMLVLLPQLAEAHGTSAGPHLAAEPVDEATTEPSGRSAPALSIVEGLAEALATTTEANTSTTCVPIAEQDCVRHICLEGQLIRVNQSQHCPQGAAPPRCGILGLAVRVGGDRCCPLWECACRCSIFPDLSFVTFDGSHVALFKEAIYILSQSPDEMLTVHVLDCKSANLGHLNWPPFCLVMLNMTHLAHQVTIDRFNRKVTVDLQPVWPPVSRYGFRIEDTGHMYMILTPSDIQIQWLHSSGLMIVEASKTSKAQGHGLCGICDGDAANDLTLKDGSVVGGAEDPAPFLDSWQVPSSLTSVGQTRFRPDSCATTDCSPCLRMVSNRTFSACHRFVPPESFCELWIRDTKYVQQPCVALTVYVAMCHKFHVCIEWRRSDYCPFLCSSDSTYQACVTACEPPKTCQDGILGPLDPEHCQVLGEGCVCSEGTILHRRHSALCIPEAKCACTDSMGVPRALGETWNSSLSGCCQHQCQAPDTIVPVDLGCPSPRPESCLRFGEVALLLPTKDPCCLGTVCVCNQTLCEGLAPTCRPGHRLLTHFQEDSCCPSYSCECDPDLCEAELVPSCRQDQILITGRLGDSCCTSYFCACGDCPDSIPECQEGEALTVHRNTTELCCPLYQCVCENFRCPQVQCGLGTALVEVWSPDRCCPYKSCECDCDTIPVPRCHLWEKSQLDEEFMHSVENVCGCAKYECVKAPVCLSRELGVMQPGQTVVELSADGVCHTSRCTTVLDPLTNFYQINTTSVLCDIHCEANQEYEHPRDLAACCGSCRNVSCLFTFPNGTTSLFLPGASWIADCARHHCSSTPLGAVLVRSPISCPPLNETECAKVGGSVVPSLEGCCRTCKEDGRSCKKVTIRMTIRKNECRSSTPVNLVSCDGRCPSASIYNYNINTYARFCKCCREVGLQRRSVQLFCATNATWVPYTVQEPTDCACQWS

Target ORF information:

RefSeq Version	NM_001277269.2
Organism	Homo sapiens(human)
Definition	Homo sapiens otogelin (OTOG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277269.2

ORF Insert Sequence:

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGGTGAGA GGGTTGTGGA CTCAGGGAGG 
TCGGGGGCTC GAGGAATGAG AAACGTTAAA GGAATGAGGA ATGGGCCCGC GCAGACTCGT 
GTTTCCTCCA GCAGCAGCCA CCAGGAGGCG ACCCTTGCCA TGGGGGACAA GGCTACAGTC 
GTGGGAGGCC AGCAGGCTGA AGCCCCAGAC TCCGTGGCCA TGTCTTCCTG GGAAAGGCGG 
CTCCATCGGG CCAAGTGTGC ACCATCCTAC TTGTTCTCCT GCTTCAATGG AGGCGAGTGT 
GTGCACCCAG CCTTCTGTGA CTGCAGACGC TTCAATGCCA CTGGACCGCG CTGCCAGATG 
GTGTACAATG CCGGCCCTGA GAGGGACAGC ATTTGCCGGG CGTGGGGGCA GCACCACGTG 
GAGACATTTG ATGGGCTCTA CTACTACCTC TCCGGAAAGG GCAGCTACAC CCTGGTGGGT 
CGCCATGAGC CCGAGGGACA GAGCTTCTCC ATCCAGGTAC ACAATGACCC GCAGTGTGGC 
TCTTCACCCT ACACCTGCTC CAGGGCTGTC AGCCTCTTCT TTGTGGGTGA GCAGGAGATC 
CATCTGGCCA AGGAGGTCAC CCATGGAGGC ATGAGGGTCC AACTGCCACA TGTCATGGGG 
AGCGCGCGTC TGCAGCAGCT TGCCGGCTAT GTCATCGTGC GGCATCAGTC AGCCTTCACA 
CTGGCCTGGG ATGGTGCCTC GGCTGTCTAC ATCAAGATGA GTCCAGAGCT TCTGGGCTGG 
ACCCATGGGC TGTGTGGGAA CAACAATGCT GACCCCAAGG ATGATCTGGT GACCAGCTCT 
GGGAAGCTGA CTGACGACGT GGTTGAGTTT GTGCACAGCT GGCAGGAGCA GGCCCCTAAC 
CAGCCTCCAG GGCCCACAAC TTCCTCCCTG CCTCGCCCAC CGTGCCTACA GCAGAACCCA 
GGAACCATGC AGGGCGTGTA CGAGCAGTGT GAGGCTCTAC TGCGGCCCCC CTTTGACGCC 
TGCCACGCCT ACGTCAGCCC TCTGCCCTTC ACAGCCAGTT GTACCAGTGA TCTCTGCCAA 
TCAATGGGTG ATGTAGCCAC CTGGTGCCGG GCACTGGCGG AGTATGCCCG GGCGTGTGCC 
CAGGCAGGGC GGCCCTTGCA AGGCTGGAGG ACCCAGCTCC GGCAATGCAC TGTGCACTGC 
AAGGAGAAGG CCTTTACCTA CAATGAGTGC ATCGCCTGCT GCCCTGCCTC CTGCCATCCC 
CGGGCATCCT GTGTGGACAG TGAGATCGCC TGTGTGGACG GCTGCTATTG CCCCAATGGG 
CTCATCTTCG AGGATGGGGG CTGCGTGGCA CCAGCTGAGT GTCCCTGTGA GTTTCACGGG 
ACTCTGTACC CACCTGGCTC TGTGGTGAAG GAAGACTGCA ATACTTGCAC ATGCACCTCA 
GGCAAGTGGG AGTGCAGCAC AGCTGTCTGC CCAGCTGAGT GCTCAGTGAC TGGTGACATT 
CACTTCACAA CCTTTGATGG CCGCCGGTAC ACGTTCCCCG CCACATGTCA GTACATCCTG 
GCCAAGAGCC GCTCTTCGGG CACCTTCACC GTGACATTGC AGAATGCCCC ATGTGGCCTG 
AACCAAGATG GAGCCTGTGT CCAGTCAGTG TCAGTGATTC TGCACCAGGA CCCTCGGAGG 
CAGGTGACCC TGACCCAGGC AGGGGATGTC CTTCTGTTTG ACCAGTACAA GATCATCCCG 
CCATACACAG ATGATGCCTT TGAGATCCGT AGGCTGTCCT CCGTGTTCCT GCGGGTGAGG 
ACGAACGTGG GCGTGCGGGT GCTCTACGAC CGTGAAGGGC TCCGACTGTA CCTGCAAGTG 
GACCAGCGAT GGGTGGAGGA TACCGTGGGC CTCTGCGGCA CCTTCAATGG CAACACGCAG 
GATGACTTCC TGTCTCCAGT GGGTGTACCT GAGAGCACCC CACAACTTTT TGGCAATTCC 
TGGAAAACAC TTTCTGCTTG CTCCCCGCTG GTCTCTGGCT CCCCTCTGGA CCCCTGCGAT 
GTGCACCTGC AAGCCGCCTC CTACTCAGTG CAGGCCTGCA GCGTGCTCAC GGGGGAGATG 
TTTGCGCCCT GCTCTGCGTT CCTGAGCCCC GTGCCCTACT TTGAGCAGTG CCGCAGGGAT 
GCCTGCCGCT GCGGGCAGCC CTGCCTGTGC GCCACACTGG CCCACTACGC CCACCTGTGC 
CGGCGCCATG GGCTCCCCGT TGATTTCCGC GCCCGCCTGC CAGCCTGTGC ACTGTCCTGT 
GAGGCCTCCA AGGAGTATAG CCCCTGCGTG GCCCCGTGTG GACGTACCTG CCAGGACCTG 
GCCAGCCCTG AGGCCTGTGG GGTTGATGGT GGCGATGACC TGAGCAGAGA CGAGTGTGTG 
GAGGGCTGTG CCTGCCCACC GGACACCTAT CTGGACACCC AGGCTGACCT CTGTGTCCCC 
CGGAACCAGT GCTCCTGCCA CTTCCAGGGA GTGGACTATC CCCCCGGAGA CAGTGACATC 
CCATCCCTGG GCCACTGCCA CTGCAAAGAT GGAGTCATGA GCTGTGATAG CAGAGCCCCA 
GCTGCTGCCT GCCCAGCAGG CCAGGTCTTC GTGAACTGCA GCGACCTGCA CACGGACCTG 
GAGCTGAGCA GGGAGAGGAC GTGTGAGCAG CAACTGCTGA ACCTGAGCGT GTCAGCCCGT 
GGCCCCTGCC TCTCGGGCTG CGCCTGTCCC CAGGGTCTGC TCAGACACGG GGATGCATGT 
TTCCTGCCAG AGGAGTGCCC CTGCACTTGG AAGGGGAAGG AGTATTTCCC TGGGGACCAG 
GTGATGTCTC CTTGCCATAC CTGTGTGTGC CAGCGGGGCT CATTCCAGTG CACCCTGCAC 
CCTTGCGCCT CCACCTGCAC TGCCTATGGG GACCGGCATT ACCGCACGTT TGATGGGCTC 
CCGTTTGACT TCGTGGGGGC ATGCAAAGTG CACCTGGTCA AGAGCACATC AGATGTCAGC 
TTCTCTGTGA TTGTAGAGAA TGTGAACTGC TACAGCTCTG GCATGATCTG CAGGAAATTT 
ATTTCCATCA ACGTTGGGAA CTCACTCATT GTCTTTGATG ATGACTCCGG AAATCCTAGT 
CCAGAGAGCT TCCTGGATGA CAAGCAGGAG GTCCACACAT GGCGAGTGGG ATTTTTCACA 
CTGGTGCATT TCCCACAGGA GCACATCACC CTCTTGTGGG ACCAGAGAAC CACAGTGCAC 
GTCCAGGCTG GGCCTCAGTG GCAGGGCCAG CTGGCGGGCC TCTGTGGGAA CTTTGACTTA 
AAAACCATCA ATGAGATGAG GACCCCGGAG AACCTAGAGC TAACTAACCC CCAGGAGTTT 
GGCAGCAGTT GGGCTGCAGT TGAGTGCCCA GACACCCTCG ATCCTCGGGA TATGTGTGTC 
CTGAATCCTC TCCGAGAACC ATTTGCCAAG AAGGAGTGCA GCATCCTGCT CAGTGAGGTG 
TTTGAGATCT GCCACCCTGT GGTTGATGTC ACTTGGTTTT ACTCAAACTG CCTGACAGAC 
ACATGTGGCT GCAGCCAGGG TGGTGACTGT GAGTGCTTCT GTGCCAGCGT CTCCGCTTAT 
GCCCACCAGT GTTGCCAGCA TGGGGTGGCT GTTGACTGGC GAACCCCCCG CCTCTGCCCG 
TATGACTGTG ACTTCTTTAA CAAAGTGCTA GGTAAGGGCC CCTATCAGCT ATCCAGCTTG 
GCAGCCGGTG GTGCTCTGGT GGGCATGAAG GCGGTGGGCG ATGACATAGT CCTAGTGAGG 
ACAGAGGATG TGGCGCCAGC AGACATTGTG AGCTTCCTGC TGACAGCTGC TCTGTACAAG 
GCCAAGGCCC ATGACCCAGA TGTGGTGTCC CTGGAGGCAG CAGACAGACC CAACTTCTTC 
CTTCACGTCA CAGCCAACGG GTCTCTGGAG CTGGCTAAGT GGCAGGGCCG TGACACCTTC 
CAACAGCATG CCTCCTTCTT GCTGCACCGG GGGACACGGC AGGCAGGCCT GGTGGCCCTG 
GAGTCCCTGG CCAAGCCCAG CTCCTTCCTC TATGTGTCGG GCGCGGTGCT GGCCCTGCGG 
CTGTACGAAC ACACAGAGGT GTTCCGCCGG GGCACACTCT TCCGCCTTCT GGATGCCAAG 
CCCTCGGGGG CTGCCTACCC CATCTGCGAG TGGCGCTACG ATGCCTGTGC CAGCCCCTGC 
TTCCAAACCT GCCGGGACCC ACGGGCAGCC AGCTGCCGGG ACGTACCCAG GGTAGAAGGC 
TGTGTCCCTG TGTGCCCCAC CCCCCAGGTC CTGGATGAAG TCACACAGAG ATGTGTCTAC 
TTGGAGGACT GTGTGGAGCC AGCAGTTTGG GTTCCCACAG AGGCCCTTGG CAATGAGACC 
CTCCCTCCCA GTCAAGGGTT GCCCACTCCC AGTGATGAGG AGCCACAGCT GTCACAGGAA 
AGCCCCAGGA CCCCCACCCA CAGGCCAGCC CTCACCCCAG CTGCCCCACT CACCACAGCC 
CTGAACCCAC CAGTGACAGC CACTGAGGAG CCAGTGGTGT CTCCAGGCCC CACCCAGACC 
ACCCTGCAGC AGCCACTGGA GCTCACTGCA TCTCAACTCC CCGCCGGCCC CACGGAGTCC 
CCAGCCAGCA AGGGAGTGAC TGCCAGCCTC CTGGCCATCC CCCATACACC AGAGTCCTCA 
TCCCTCCCTG TTGCACTGCA GACACCCACA CCTGGCATGG TGTCAGGTGC CATGGAGACA 
ACAAGGGTGA CTGTGATCTT TGCAGGAAGC CCTAACATCA CAGTCTCCTC CCGGTCGCCC 
CCTGCCCCTC GCTTCCCGCT CATGACCAAG GCTGTGACAG TCCGAGGCCA TGGCTCCTTG 
CCTGTTAGGA CGACACCCCC ACAGCCCTCC TTGACAGCAA GTCCCTCCTC CAGACCTGTG 
GCTTCCCCTG GAGCCATCTC CAGGTCCCCC ACCTCCTCGG GATCCCACAA GGCTGTGCTG 
ACACCTGCAG TAACTAAGGT CATAAGCAGG ACAGGGGTCC CCCAGCCCAC CCAGGCCCAG 
AGTGCTTCAA GTCCCAGCAC CCCTCTAACT GTGGCTGGAA CAGCAGCAGA ACAGGTTCCT 
GTCAGTCCCC TTGCAACCAG GAGCTTGGAG ATAGTGCTAT CCACAGAGAA GGGCGAAGCC 
GGGCACAGCC AGCCCATGGG CTCGCCTGCC TCCCCACAGC CACACCCACT CCCCTCTGCA 
CCACCCCGCC CAGCCCAGCA TACCACCATG GCCACCAGGT CTCCAGCTCT GCCCCCAGAG 
ACCCCAGCTG CCGCCAGCCT GTCAACAGCC ACTGATGGGC TGGCAGCCAC ACCCTTCATG 
TCCCTTGAGT CAACTCGTCC CTCCCAGCTC CTCTCTGGCC TGCCTCCCGA CACCAGCCTG 
CCCCTGGCCA AGGTGGGCAC ATCTGCCCCA GTGGCCACAC CCGGCCCCAA AGCCTCTGTC 
ATCACCACTC CACTCCAGCC ACAGGCCACG ACTCTGCCTG CTCAGACACT TAGCCCAGTA 
CTGCCTTTCA CTCCAGCAGC AATGACCCAG GCGCACCCAC CCACTCACAT AGCACCCCCA 
GCAGCAGGCA CAGCTCCAGG CCTGCTGCTG GGAGCCACAT TGCCAACCTC TGGAGTCCTG 
CCTGTGGCTG AGGGCACGGC CTCCATGGTA TCTGTTGTCC CACGAAAGAG CACCACAGGG 
AAGGTGGCCA TCCTATCCAA GCAAGTGTCT CTGCCCACTT CCATGTATGG TTCTGCAGAG 
GGTGGGCCCA CAGAGCTCAC GCCTGCTACG AGCCACCCTC TCACGCCCTT GGTGGCTGAG 
CCCGAGGGAG CCCAGGCAGG CACAGCTCTG CCAGTGCCCA CATCCTATGC CCTGAGCCGT 
GTCTCAGCCA GGACGGCCCC CCAAGACAGC ATGCTGGTTC TGTTGCCTCA GCTGGCTGAG 
GCCCATGGAA CCTCGGCAGG GCCTCACCTG GCAGCAGAGC CGGTGGACGA GGCCACCACA 
GAACCATCTG GGCGCTCAGC CCCAGCCCTG AGCATCGTAG AGGGTTTGGC GGAGGCTTTG 
GCAACTACCA CTGAGGCCAA TACATCCACC ACCTGTGTTC CAATCGCCGA GCAGGACTGC 
GTCCGCCACA TCTGCCTGGA GGGCCAGCTG ATTCGCGTGA ATCAGTCCCA GCACTGTCCC 
CAGGGTGCTG CTCCCCCTCG CTGTGGGATC CTGGGCCTCG CCGTGCGGGT GGGTGGGGAC 
CGCTGCTGCC CACTCTGGGA GTGTGCCTGC CGGTGCTCAA TCTTCCCTGA CCTGAGCTTC 
GTGACCTTCG ATGGGAGCCA CGTAGCTCTG TTCAAGGAGG CCATCTACAT CCTCAGCCAG 
AGCCCAGATG AAATGCTCAC CGTCCATGTA CTGGACTGCA AAAGTGCCAA CCTGGGGCAC 
CTGAACTGGC CCCCGTTCTG TCTGGTGATG TTGAACATGA CTCACTTGGC CCATCAGGTC 
ACTATTGATC GCTTCAACCG AAAGGTGACT GTGGACTTGC AGCCTGTGTG GCCACCGGTG 
AGCAGGTATG GATTCAGAAT TGAGGACACA GGCCACATGT ACATGATCCT GACTCCCTCA 
GACATCCAGA TCCAGTGGCT CCACAGCTCA GGACTCATGA TCGTGGAGGC CAGCAAAACC 
AGCAAGGCCC AGGGCCATGG CCTGTGCGGT ATCTGTGATG GAGATGCAGC CAATGACCTT 
ACCCTGAAGG ATGGCTCAGT GGTGGGTGGG GCTGAGGACC CTGCTCCCTT TCTGGACAGC 
TGGCAGGTGC CCAGCTCCCT GACCTCAGTG GGCCAGACCC GCTTCCGCCC AGACAGCTGC 
GCCACAACTG ACTGCTCGCC CTGCCTTCGC ATGGTGTCCA ACCGCACCTT CAGTGCCTGC 
CACCGCTTTG TGCCTCCGGA GTCATTCTGT GAGCTGTGGA TCCGGGACAC CAAGTACGTG 
CAGCAGCCCT GCGTGGCCCT GACTGTGTAC GTGGCCATGT GCCACAAATT TCATGTGTGC 
ATCGAGTGGC GGCGCTCTGA CTACTGCCCC TTCCTGTGCT CCAGCGACTC CACATACCAG 
GCATGTGTGA CAGCCTGTGA GCCACCCAAG ACATGCCAGG ATGGGATACT AGGGCCTCTG 
GACCCAGAGC ACTGCCAGGT GCTGGGCGAG GGCTGCGTCT GCTCCGAGGG CACCATCTTA 
CACCGGCGCC ACTCTGCACT CTGCATCCCG GAGGCCAAGT GCGCCTGCAC TGACAGCATG 
GGGGTGCCGA GGGCCCTGGG GGAGACCTGG AACAGCTCCC TCAGCGGCTG CTGCCAGCAC 
CAGTGCCAAG CCCCAGACAC CATTGTCCCG GTGGATCTGG GCTGCCCCAG TCCCCGCCCT 
GAGAGCTGCC TGCGATTCGG GGAGGTGGCC TTGCTCCTAC CCACCAAGGA CCCCTGCTGC 
CTGGGGACTG TCTGTGTGTG TAACCAGACT CTGTGTGAGG GTCTCGCCCC CACATGCCGC 
CCAGGCCACC GCCTCCTCAC CCACTTCCAG GAGGACTCCT GCTGCCCCAG CTACAGCTGT 
GAGTGTGACC CAGATCTCTG TGAGGCAGAG CTGGTCCCCA GCTGCCGACA GGACCAGATC 
CTGATCACGG GCCGCCTGGG GGACTCCTGC TGCACCTCCT ACTTCTGCGC CTGTGGTGAC 
TGTCCAGACT CCATCCCCGA ATGTCAAGAA GGGGAGGCGC TCACTGTGCA CAGGAATACC 
ACGGAACTCT GCTGCCCTCT GTACCAGTGT GTGTGTGAGA ACTTCCGCTG TCCCCAAGTG 
CAGTGTGGCC TGGGCACTGC CCTGGTGGAG GTGTGGAGCC CCGACCGCTG CTGCCCCTAC 
AAATCCTGTG AATGTGACTG TGACACAATC CCGGTGCCCC GGTGCCATCT GTGGGAGAAA 
TCCCAGCTGG ATGAGGAGTT CATGCACAGC GTGGAGAATG TGTGTGGCTG CGCCAAGTAC 
GAGTGTGTGA AGGCCCCGGT GTGTCTGAGC CGCGAGCTGG GTGTGATGCA GCCCGGCCAG 
ACAGTGGTGG AGCTCTCAGC AGATGGCGTG TGCCACACCT CCCGCTGCAC CACCGTGCTC 
GACCCTCTCA CCAACTTCTA CCAGATCAAC ACCACCTCCG TGCTCTGTGA CATCCACTGT 
GAGGCGAACC AGGAGTACGA GCACCCGCGG GACCTCGCTG CCTGCTGCGG CTCCTGCAGG 
AACGTGTCCT GTCTCTTCAC CTTCCCCAAT GGCACCACCT CCCTGTTCTT GCCCGGGGCA 
TCCTGGATCG CAGACTGCGC CCGCCACCAC TGCAGCAGCA CGCCCCTGGG TGCCGTGCTG 
GTCCGCTCTC CCATAAGCTG CCCACCGCTC AATGAGACTG AGTGTGCCAA GGTTGGGGGT 
TCCGTGGTAC CTTCCTTGGA AGGATGCTGC AGGACCTGTA AGGAGGATGG GCGCTCCTGC 
AAGAAGGTGA CCATCCGCAT GACCATCCGC AAGAATGAAT GCAGGAGCAG CACCCCTGTG 
AACCTAGTGT CCTGCGATGG GAGGTGCCCA TCCGCCAGCA TCTACAACTA CAACATCAAC 
ACCTATGCCC GATTCTGCAA GTGCTGCCGT GAGGTGGGCC TGCAGCGGCG CTCTGTGCAG 
CTCTTCTGTG CCACCAATGC CACCTGGGTG CCCTATACAG TGCAGGAGCC CACCGACTGT 
GCCTGCCAGT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

OTOG ( NM_001277269.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001264198.1 Homo sapiens otogelin (OTOG), transcript variant 1, mRNA.

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CloneID	OHu27814
Clone ID Related Accession (Same CDS sequence)	NM_001277269.2 , NM_001277269.1
Accession Version	NM_001277269.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-30
Organism	Homo sapiens(human)
Product	otogelin isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC124799.5, EG328175.1, EG328176.1, DV080384.1, DB081210.1, DV080242.1, DV080241.1, DV080216.1, DV080434.1, AK128214.1 and DV080398.1. This sequence is a reference standard in the RefSeqGene project. On Mar 27, 2013 this sequence version replaced XM_291816.8. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The exon combination of this transcript is inferred based on orthologous transcript alignments, and on data in PMID:23122587. CCDS Note: This CCDS representation lacks full-length human transcript support. Its exon combination is therefore inferred, but it is supported by partial human transcript alignments and data in PMID:23122587. ##RefSeq-Attributes-START## inferred exon combination :: PMID: 23122587 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGGTGAGA GGGTTGTGGA CTCAGGGAGG 
TCGGGGGCTC GAGGAATGAG AAACGTTAAA GGAATGAGGA ATGGGCCCGC GCAGACTCGT 
GTTTCCTCCA GCAGCAGCCA CCAGGAGGCG ACCCTTGCCA TGGGGGACAA GGCTACAGTC 
GTGGGAGGCC AGCAGGCTGA AGCCCCAGAC TCCGTGGCCA TGTCTTCCTG GGAAAGGCGG 
CTCCATCGGG CCAAGTGTGC ACCATCCTAC TTGTTCTCCT GCTTCAATGG AGGCGAGTGT 
GTGCACCCAG CCTTCTGTGA CTGCAGACGC TTCAATGCCA CTGGACCGCG CTGCCAGATG 
GTGTACAATG CCGGCCCTGA GAGGGACAGC ATTTGCCGGG CGTGGGGGCA GCACCACGTG 
GAGACATTTG ATGGGCTCTA CTACTACCTC TCCGGAAAGG GCAGCTACAC CCTGGTGGGT 
CGCCATGAGC CCGAGGGACA GAGCTTCTCC ATCCAGGTAC ACAATGACCC GCAGTGTGGC 
TCTTCACCCT ACACCTGCTC CAGGGCTGTC AGCCTCTTCT TTGTGGGTGA GCAGGAGATC 
CATCTGGCCA AGGAGGTCAC CCATGGAGGC ATGAGGGTCC AACTGCCACA TGTCATGGGG 
AGCGCGCGTC TGCAGCAGCT TGCCGGCTAT GTCATCGTGC GGCATCAGTC AGCCTTCACA 
CTGGCCTGGG ATGGTGCCTC GGCTGTCTAC ATCAAGATGA GTCCAGAGCT TCTGGGCTGG 
ACCCATGGGC TGTGTGGGAA CAACAATGCT GACCCCAAGG ATGATCTGGT GACCAGCTCT 
GGGAAGCTGA CTGACGACGT GGTTGAGTTT GTGCACAGCT GGCAGGAGCA GGCCCCTAAC 
CAGCCTCCAG GGCCCACAAC TTCCTCCCTG CCTCGCCCAC CGTGCCTACA GCAGAACCCA 
GGAACCATGC AGGGCGTGTA CGAGCAGTGT GAGGCTCTAC TGCGGCCCCC CTTTGACGCC 
TGCCACGCCT ACGTCAGCCC TCTGCCCTTC ACAGCCAGTT GTACCAGTGA TCTCTGCCAA 
TCAATGGGTG ATGTAGCCAC CTGGTGCCGG GCACTGGCGG AGTATGCCCG GGCGTGTGCC 
CAGGCAGGGC GGCCCTTGCA AGGCTGGAGG ACCCAGCTCC GGCAATGCAC TGTGCACTGC 
AAGGAGAAGG CCTTTACCTA CAATGAGTGC ATCGCCTGCT GCCCTGCCTC CTGCCATCCC 
CGGGCATCCT GTGTGGACAG TGAGATCGCC TGTGTGGACG GCTGCTATTG CCCCAATGGG 
CTCATCTTCG AGGATGGGGG CTGCGTGGCA CCAGCTGAGT GTCCCTGTGA GTTTCACGGG 
ACTCTGTACC CACCTGGCTC TGTGGTGAAG GAAGACTGCA ATACTTGCAC ATGCACCTCA 
GGCAAGTGGG AGTGCAGCAC AGCTGTCTGC CCAGCTGAGT GCTCAGTGAC TGGTGACATT 
CACTTCACAA CCTTTGATGG CCGCCGGTAC ACGTTCCCCG CCACATGTCA GTACATCCTG 
GCCAAGAGCC GCTCTTCGGG CACCTTCACC GTGACATTGC AGAATGCCCC ATGTGGCCTG 
AACCAAGATG GAGCCTGTGT CCAGTCAGTG TCAGTGATTC TGCACCAGGA CCCTCGGAGG 
CAGGTGACCC TGACCCAGGC AGGGGATGTC CTTCTGTTTG ACCAGTACAA GATCATCCCG 
CCATACACAG ATGATGCCTT TGAGATCCGT AGGCTGTCCT CCGTGTTCCT GCGGGTGAGG 
ACGAACGTGG GCGTGCGGGT GCTCTACGAC CGTGAAGGGC TCCGACTGTA CCTGCAAGTG 
GACCAGCGAT GGGTGGAGGA TACCGTGGGC CTCTGCGGCA CCTTCAATGG CAACACGCAG 
GATGACTTCC TGTCTCCAGT GGGTGTACCT GAGAGCACCC CACAACTTTT TGGCAATTCC 
TGGAAAACAC TTTCTGCTTG CTCCCCGCTG GTCTCTGGCT CCCCTCTGGA CCCCTGCGAT 
GTGCACCTGC AAGCCGCCTC CTACTCAGTG CAGGCCTGCA GCGTGCTCAC GGGGGAGATG 
TTTGCGCCCT GCTCTGCGTT CCTGAGCCCC GTGCCCTACT TTGAGCAGTG CCGCAGGGAT 
GCCTGCCGCT GCGGGCAGCC CTGCCTGTGC GCCACACTGG CCCACTACGC CCACCTGTGC 
CGGCGCCATG GGCTCCCCGT TGATTTCCGC GCCCGCCTGC CAGCCTGTGC ACTGTCCTGT 
GAGGCCTCCA AGGAGTATAG CCCCTGCGTG GCCCCGTGTG GACGTACCTG CCAGGACCTG 
GCCAGCCCTG AGGCCTGTGG GGTTGATGGT GGCGATGACC TGAGCAGAGA CGAGTGTGTG 
GAGGGCTGTG CCTGCCCACC GGACACCTAT CTGGACACCC AGGCTGACCT CTGTGTCCCC 
CGGAACCAGT GCTCCTGCCA CTTCCAGGGA GTGGACTATC CCCCCGGAGA CAGTGACATC 
CCATCCCTGG GCCACTGCCA CTGCAAAGAT GGAGTCATGA GCTGTGATAG CAGAGCCCCA 
GCTGCTGCCT GCCCAGCAGG CCAGGTCTTC GTGAACTGCA GCGACCTGCA CACGGACCTG 
GAGCTGAGCA GGGAGAGGAC GTGTGAGCAG CAACTGCTGA ACCTGAGCGT GTCAGCCCGT 
GGCCCCTGCC TCTCGGGCTG CGCCTGTCCC CAGGGTCTGC TCAGACACGG GGATGCATGT 
TTCCTGCCAG AGGAGTGCCC CTGCACTTGG AAGGGGAAGG AGTATTTCCC TGGGGACCAG 
GTGATGTCTC CTTGCCATAC CTGTGTGTGC CAGCGGGGCT CATTCCAGTG CACCCTGCAC 
CCTTGCGCCT CCACCTGCAC TGCCTATGGG GACCGGCATT ACCGCACGTT TGATGGGCTC 
CCGTTTGACT TCGTGGGGGC ATGCAAAGTG CACCTGGTCA AGAGCACATC AGATGTCAGC 
TTCTCTGTGA TTGTAGAGAA TGTGAACTGC TACAGCTCTG GCATGATCTG CAGGAAATTT 
ATTTCCATCA ACGTTGGGAA CTCACTCATT GTCTTTGATG ATGACTCCGG AAATCCTAGT 
CCAGAGAGCT TCCTGGATGA CAAGCAGGAG GTCCACACAT GGCGAGTGGG ATTTTTCACA 
CTGGTGCATT TCCCACAGGA GCACATCACC CTCTTGTGGG ACCAGAGAAC CACAGTGCAC 
GTCCAGGCTG GGCCTCAGTG GCAGGGCCAG CTGGCGGGCC TCTGTGGGAA CTTTGACTTA 
AAAACCATCA ATGAGATGAG GACCCCGGAG AACCTAGAGC TAACTAACCC CCAGGAGTTT 
GGCAGCAGTT GGGCTGCAGT TGAGTGCCCA GACACCCTCG ATCCTCGGGA TATGTGTGTC 
CTGAATCCTC TCCGAGAACC ATTTGCCAAG AAGGAGTGCA GCATCCTGCT CAGTGAGGTG 
TTTGAGATCT GCCACCCTGT GGTTGATGTC ACTTGGTTTT ACTCAAACTG CCTGACAGAC 
ACATGTGGCT GCAGCCAGGG TGGTGACTGT GAGTGCTTCT GTGCCAGCGT CTCCGCTTAT 
GCCCACCAGT GTTGCCAGCA TGGGGTGGCT GTTGACTGGC GAACCCCCCG CCTCTGCCCG 
TATGACTGTG ACTTCTTTAA CAAAGTGCTA GGTAAGGGCC CCTATCAGCT ATCCAGCTTG 
GCAGCCGGTG GTGCTCTGGT GGGCATGAAG GCGGTGGGCG ATGACATAGT CCTAGTGAGG 
ACAGAGGATG TGGCGCCAGC AGACATTGTG AGCTTCCTGC TGACAGCTGC TCTGTACAAG 
GCCAAGGCCC ATGACCCAGA TGTGGTGTCC CTGGAGGCAG CAGACAGACC CAACTTCTTC 
CTTCACGTCA CAGCCAACGG GTCTCTGGAG CTGGCTAAGT GGCAGGGCCG TGACACCTTC 
CAACAGCATG CCTCCTTCTT GCTGCACCGG GGGACACGGC AGGCAGGCCT GGTGGCCCTG 
GAGTCCCTGG CCAAGCCCAG CTCCTTCCTC TATGTGTCGG GCGCGGTGCT GGCCCTGCGG 
CTGTACGAAC ACACAGAGGT GTTCCGCCGG GGCACACTCT TCCGCCTTCT GGATGCCAAG 
CCCTCGGGGG CTGCCTACCC CATCTGCGAG TGGCGCTACG ATGCCTGTGC CAGCCCCTGC 
TTCCAAACCT GCCGGGACCC ACGGGCAGCC AGCTGCCGGG ACGTACCCAG GGTAGAAGGC 
TGTGTCCCTG TGTGCCCCAC CCCCCAGGTC CTGGATGAAG TCACACAGAG ATGTGTCTAC 
TTGGAGGACT GTGTGGAGCC AGCAGTTTGG GTTCCCACAG AGGCCCTTGG CAATGAGACC 
CTCCCTCCCA GTCAAGGGTT GCCCACTCCC AGTGATGAGG AGCCACAGCT GTCACAGGAA 
AGCCCCAGGA CCCCCACCCA CAGGCCAGCC CTCACCCCAG CTGCCCCACT CACCACAGCC 
CTGAACCCAC CAGTGACAGC CACTGAGGAG CCAGTGGTGT CTCCAGGCCC CACCCAGACC 
ACCCTGCAGC AGCCACTGGA GCTCACTGCA TCTCAACTCC CCGCCGGCCC CACGGAGTCC 
CCAGCCAGCA AGGGAGTGAC TGCCAGCCTC CTGGCCATCC CCCATACACC AGAGTCCTCA 
TCCCTCCCTG TTGCACTGCA GACACCCACA CCTGGCATGG TGTCAGGTGC CATGGAGACA 
ACAAGGGTGA CTGTGATCTT TGCAGGAAGC CCTAACATCA CAGTCTCCTC CCGGTCGCCC 
CCTGCCCCTC GCTTCCCGCT CATGACCAAG GCTGTGACAG TCCGAGGCCA TGGCTCCTTG 
CCTGTTAGGA CGACACCCCC ACAGCCCTCC TTGACAGCAA GTCCCTCCTC CAGACCTGTG 
GCTTCCCCTG GAGCCATCTC CAGGTCCCCC ACCTCCTCGG GATCCCACAA GGCTGTGCTG 
ACACCTGCAG TAACTAAGGT CATAAGCAGG ACAGGGGTCC CCCAGCCCAC CCAGGCCCAG 
AGTGCTTCAA GTCCCAGCAC CCCTCTAACT GTGGCTGGAA CAGCAGCAGA ACAGGTTCCT 
GTCAGTCCCC TTGCAACCAG GAGCTTGGAG ATAGTGCTAT CCACAGAGAA GGGCGAAGCC 
GGGCACAGCC AGCCCATGGG CTCGCCTGCC TCCCCACAGC CACACCCACT CCCCTCTGCA 
CCACCCCGCC CAGCCCAGCA TACCACCATG GCCACCAGGT CTCCAGCTCT GCCCCCAGAG 
ACCCCAGCTG CCGCCAGCCT GTCAACAGCC ACTGATGGGC TGGCAGCCAC ACCCTTCATG 
TCCCTTGAGT CAACTCGTCC CTCCCAGCTC CTCTCTGGCC TGCCTCCCGA CACCAGCCTG 
CCCCTGGCCA AGGTGGGCAC ATCTGCCCCA GTGGCCACAC CCGGCCCCAA AGCCTCTGTC 
ATCACCACTC CACTCCAGCC ACAGGCCACG ACTCTGCCTG CTCAGACACT TAGCCCAGTA 
CTGCCTTTCA CTCCAGCAGC AATGACCCAG GCGCACCCAC CCACTCACAT AGCACCCCCA 
GCAGCAGGCA CAGCTCCAGG CCTGCTGCTG GGAGCCACAT TGCCAACCTC TGGAGTCCTG 
CCTGTGGCTG AGGGCACGGC CTCCATGGTA TCTGTTGTCC CACGAAAGAG CACCACAGGG 
AAGGTGGCCA TCCTATCCAA GCAAGTGTCT CTGCCCACTT CCATGTATGG TTCTGCAGAG 
GGTGGGCCCA CAGAGCTCAC GCCTGCTACG AGCCACCCTC TCACGCCCTT GGTGGCTGAG 
CCCGAGGGAG CCCAGGCAGG CACAGCTCTG CCAGTGCCCA CATCCTATGC CCTGAGCCGT 
GTCTCAGCCA GGACGGCCCC CCAAGACAGC ATGCTGGTTC TGTTGCCTCA GCTGGCTGAG 
GCCCATGGAA CCTCGGCAGG GCCTCACCTG GCAGCAGAGC CGGTGGACGA GGCCACCACA 
GAACCATCTG GGCGCTCAGC CCCAGCCCTG AGCATCGTAG AGGGTTTGGC GGAGGCTTTG 
GCAACTACCA CTGAGGCCAA TACATCCACC ACCTGTGTTC CAATCGCCGA GCAGGACTGC 
GTCCGCCACA TCTGCCTGGA GGGCCAGCTG ATTCGCGTGA ATCAGTCCCA GCACTGTCCC 
CAGGGTGCTG CTCCCCCTCG CTGTGGGATC CTGGGCCTCG CCGTGCGGGT GGGTGGGGAC 
CGCTGCTGCC CACTCTGGGA GTGTGCCTGC CGGTGCTCAA TCTTCCCTGA CCTGAGCTTC 
GTGACCTTCG ATGGGAGCCA CGTAGCTCTG TTCAAGGAGG CCATCTACAT CCTCAGCCAG 
AGCCCAGATG AAATGCTCAC CGTCCATGTA CTGGACTGCA AAAGTGCCAA CCTGGGGCAC 
CTGAACTGGC CCCCGTTCTG TCTGGTGATG TTGAACATGA CTCACTTGGC CCATCAGGTC 
ACTATTGATC GCTTCAACCG AAAGGTGACT GTGGACTTGC AGCCTGTGTG GCCACCGGTG 
AGCAGGTATG GATTCAGAAT TGAGGACACA GGCCACATGT ACATGATCCT GACTCCCTCA 
GACATCCAGA TCCAGTGGCT CCACAGCTCA GGACTCATGA TCGTGGAGGC CAGCAAAACC 
AGCAAGGCCC AGGGCCATGG CCTGTGCGGT ATCTGTGATG GAGATGCAGC CAATGACCTT 
ACCCTGAAGG ATGGCTCAGT GGTGGGTGGG GCTGAGGACC CTGCTCCCTT TCTGGACAGC 
TGGCAGGTGC CCAGCTCCCT GACCTCAGTG GGCCAGACCC GCTTCCGCCC AGACAGCTGC 
GCCACAACTG ACTGCTCGCC CTGCCTTCGC ATGGTGTCCA ACCGCACCTT CAGTGCCTGC 
CACCGCTTTG TGCCTCCGGA GTCATTCTGT GAGCTGTGGA TCCGGGACAC CAAGTACGTG 
CAGCAGCCCT GCGTGGCCCT GACTGTGTAC GTGGCCATGT GCCACAAATT TCATGTGTGC 
ATCGAGTGGC GGCGCTCTGA CTACTGCCCC TTCCTGTGCT CCAGCGACTC CACATACCAG 
GCATGTGTGA CAGCCTGTGA GCCACCCAAG ACATGCCAGG ATGGGATACT AGGGCCTCTG 
GACCCAGAGC ACTGCCAGGT GCTGGGCGAG GGCTGCGTCT GCTCCGAGGG CACCATCTTA 
CACCGGCGCC ACTCTGCACT CTGCATCCCG GAGGCCAAGT GCGCCTGCAC TGACAGCATG 
GGGGTGCCGA GGGCCCTGGG GGAGACCTGG AACAGCTCCC TCAGCGGCTG CTGCCAGCAC 
CAGTGCCAAG CCCCAGACAC CATTGTCCCG GTGGATCTGG GCTGCCCCAG TCCCCGCCCT 
GAGAGCTGCC TGCGATTCGG GGAGGTGGCC TTGCTCCTAC CCACCAAGGA CCCCTGCTGC 
CTGGGGACTG TCTGTGTGTG TAACCAGACT CTGTGTGAGG GTCTCGCCCC CACATGCCGC 
CCAGGCCACC GCCTCCTCAC CCACTTCCAG GAGGACTCCT GCTGCCCCAG CTACAGCTGT 
GAGTGTGACC CAGATCTCTG TGAGGCAGAG CTGGTCCCCA GCTGCCGACA GGACCAGATC 
CTGATCACGG GCCGCCTGGG GGACTCCTGC TGCACCTCCT ACTTCTGCGC CTGTGGTGAC 
TGTCCAGACT CCATCCCCGA ATGTCAAGAA GGGGAGGCGC TCACTGTGCA CAGGAATACC 
ACGGAACTCT GCTGCCCTCT GTACCAGTGT GTGTGTGAGA ACTTCCGCTG TCCCCAAGTG 
CAGTGTGGCC TGGGCACTGC CCTGGTGGAG GTGTGGAGCC CCGACCGCTG CTGCCCCTAC 
AAATCCTGTG AATGTGACTG TGACACAATC CCGGTGCCCC GGTGCCATCT GTGGGAGAAA 
TCCCAGCTGG ATGAGGAGTT CATGCACAGC GTGGAGAATG TGTGTGGCTG CGCCAAGTAC 
GAGTGTGTGA AGGCCCCGGT GTGTCTGAGC CGCGAGCTGG GTGTGATGCA GCCCGGCCAG 
ACAGTGGTGG AGCTCTCAGC AGATGGCGTG TGCCACACCT CCCGCTGCAC CACCGTGCTC 
GACCCTCTCA CCAACTTCTA CCAGATCAAC ACCACCTCCG TGCTCTGTGA CATCCACTGT 
GAGGCGAACC AGGAGTACGA GCACCCGCGG GACCTCGCTG CCTGCTGCGG CTCCTGCAGG 
AACGTGTCCT GTCTCTTCAC CTTCCCCAAT GGCACCACCT CCCTGTTCTT GCCCGGGGCA 
TCCTGGATCG CAGACTGCGC CCGCCACCAC TGCAGCAGCA CGCCCCTGGG TGCCGTGCTG 
GTCCGCTCTC CCATAAGCTG CCCACCGCTC AATGAGACTG AGTGTGCCAA GGTTGGGGGT 
TCCGTGGTAC CTTCCTTGGA AGGATGCTGC AGGACCTGTA AGGAGGATGG GCGCTCCTGC 
AAGAAGGTGA CCATCCGCAT GACCATCCGC AAGAATGAAT GCAGGAGCAG CACCCCTGTG 
AACCTAGTGT CCTGCGATGG GAGGTGCCCA TCCGCCAGCA TCTACAACTA CAACATCAAC 
ACCTATGCCC GATTCTGCAA GTGCTGCCGT GAGGTGGGCC TGCAGCGGCG CTCTGTGCAG 
CTCTTCTGTG CCACCAATGC CACCTGGGTG CCCTATACAG TGCAGGAGCC CACCGACTGT 
GCCTGCCAGT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264198.1
CDS	1..8778
Translation	MGVLASALCWLLCVWLPWGEQAAESLRVQRLGERVVDSGRSGARGMRNVKGMRNGPAQTRVSSSSSHQEATLAMGDKATVVGGQQAEAPDSVAMSSWERRLHRAKCAPSYLFSCFNGGECVHPAFCDCRRFNATGPRCQMVYNAGPERDSICRAWGQHHVETFDGLYYYLSGKGSYTLVGRHEPEGQSFSIQVHNDPQCGSSPYTCSRAVSLFFVGEQEIHLAKEVTHGGMRVQLPHVMGSARLQQLAGYVIVRHQSAFTLAWDGASAVYIKMSPELLGWTHGLCGNNNADPKDDLVTSSGKLTDDVVEFVHSWQEQAPNQPPGPTTSSLPRPPCLQQNPGTMQGVYEQCEALLRPPFDACHAYVSPLPFTASCTSDLCQSMGDVATWCRALAEYARACAQAGRPLQGWRTQLRQCTVHCKEKAFTYNECIACCPASCHPRASCVDSEIACVDGCYCPNGLIFEDGGCVAPAECPCEFHGTLYPPGSVVKEDCNTCTCTSGKWECSTAVCPAECSVTGDIHFTTFDGRRYTFPATCQYILAKSRSSGTFTVTLQNAPCGLNQDGACVQSVSVILHQDPRRQVTLTQAGDVLLFDQYKIIPPYTDDAFEIRRLSSVFLRVRTNVGVRVLYDREGLRLYLQVDQRWVEDTVGLCGTFNGNTQDDFLSPVGVPESTPQLFGNSWKTLSACSPLVSGSPLDPCDVHLQAASYSVQACSVLTGEMFAPCSAFLSPVPYFEQCRRDACRCGQPCLCATLAHYAHLCRRHGLPVDFRARLPACALSCEASKEYSPCVAPCGRTCQDLASPEACGVDGGDDLSRDECVEGCACPPDTYLDTQADLCVPRNQCSCHFQGVDYPPGDSDIPSLGHCHCKDGVMSCDSRAPAAACPAGQVFVNCSDLHTDLELSRERTCEQQLLNLSVSARGPCLSGCACPQGLLRHGDACFLPEECPCTWKGKEYFPGDQVMSPCHTCVCQRGSFQCTLHPCASTCTAYGDRHYRTFDGLPFDFVGACKVHLVKSTSDVSFSVIVENVNCYSSGMICRKFISINVGNSLIVFDDDSGNPSPESFLDDKQEVHTWRVGFFTLVHFPQEHITLLWDQRTTVHVQAGPQWQGQLAGLCGNFDLKTINEMRTPENLELTNPQEFGSSWAAVECPDTLDPRDMCVLNPLREPFAKKECSILLSEVFEICHPVVDVTWFYSNCLTDTCGCSQGGDCECFCASVSAYAHQCCQHGVAVDWRTPRLCPYDCDFFNKVLGKGPYQLSSLAAGGALVGMKAVGDDIVLVRTEDVAPADIVSFLLTAALYKAKAHDPDVVSLEAADRPNFFLHVTANGSLELAKWQGRDTFQQHASFLLHRGTRQAGLVALESLAKPSSFLYVSGAVLALRLYEHTEVFRRGTLFRLLDAKPSGAAYPICEWRYDACASPCFQTCRDPRAASCRDVPRVEGCVPVCPTPQVLDEVTQRCVYLEDCVEPAVWVPTEALGNETLPPSQGLPTPSDEEPQLSQESPRTPTHRPALTPAAPLTTALNPPVTATEEPVVSPGPTQTTLQQPLELTASQLPAGPTESPASKGVTASLLAIPHTPESSSLPVALQTPTPGMVSGAMETTRVTVIFAGSPNITVSSRSPPAPRFPLMTKAVTVRGHGSLPVRTTPPQPSLTASPSSRPVASPGAISRSPTSSGSHKAVLTPAVTKVISRTGVPQPTQAQSASSPSTPLTVAGTAAEQVPVSPLATRSLEIVLSTEKGEAGHSQPMGSPASPQPHPLPSAPPRPAQHTTMATRSPALPPETPAAASLSTATDGLAATPFMSLESTRPSQLLSGLPPDTSLPLAKVGTSAPVATPGPKASVITTPLQPQATTLPAQTLSPVLPFTPAAMTQAHPPTHIAPPAAGTAPGLLLGATLPTSGVLPVAEGTASMVSVVPRKSTTGKVAILSKQVSLPTSMYGSAEGGPTELTPATSHPLTPLVAEPEGAQAGTALPVPTSYALSRVSARTAPQDSMLVLLPQLAEAHGTSAGPHLAAEPVDEATTEPSGRSAPALSIVEGLAEALATTTEANTSTTCVPIAEQDCVRHICLEGQLIRVNQSQHCPQGAAPPRCGILGLAVRVGGDRCCPLWECACRCSIFPDLSFVTFDGSHVALFKEAIYILSQSPDEMLTVHVLDCKSANLGHLNWPPFCLVMLNMTHLAHQVTIDRFNRKVTVDLQPVWPPVSRYGFRIEDTGHMYMILTPSDIQIQWLHSSGLMIVEASKTSKAQGHGLCGICDGDAANDLTLKDGSVVGGAEDPAPFLDSWQVPSSLTSVGQTRFRPDSCATTDCSPCLRMVSNRTFSACHRFVPPESFCELWIRDTKYVQQPCVALTVYVAMCHKFHVCIEWRRSDYCPFLCSSDSTYQACVTACEPPKTCQDGILGPLDPEHCQVLGEGCVCSEGTILHRRHSALCIPEAKCACTDSMGVPRALGETWNSSLSGCCQHQCQAPDTIVPVDLGCPSPRPESCLRFGEVALLLPTKDPCCLGTVCVCNQTLCEGLAPTCRPGHRLLTHFQEDSCCPSYSCECDPDLCEAELVPSCRQDQILITGRLGDSCCTSYFCACGDCPDSIPECQEGEALTVHRNTTELCCPLYQCVCENFRCPQVQCGLGTALVEVWSPDRCCPYKSCECDCDTIPVPRCHLWEKSQLDEEFMHSVENVCGCAKYECVKAPVCLSRELGVMQPGQTVVELSADGVCHTSRCTTVLDPLTNFYQINTTSVLCDIHCEANQEYEHPRDLAACCGSCRNVSCLFTFPNGTTSLFLPGASWIADCARHHCSSTPLGAVLVRSPISCPPLNETECAKVGGSVVPSLEGCCRTCKEDGRSCKKVTIRMTIRKNECRSSTPVNLVSCDGRCPSASIYNYNINTYARFCKCCREVGLQRRSVQLFCATNATWVPYTVQEPTDCACQWS

Target ORF information:

RefSeq Version	NM_001277269.1
Organism	Homo sapiens(human)
Definition	Homo sapiens otogelin (OTOG), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277269.1

ORF Insert Sequence:

ATGGGAGTCC TGGCGTCTGC GCTCTGCTGG CTGCTTTGTG TCTGGCTGCC CTGGGGTGAG 
CAGGCAGCCG AGTCCCTGCG GGTGCAGCGC CTCGGTGAGA GGGTTGTGGA CTCAGGGAGG 
TCGGGGGCTC GAGGAATGAG AAACGTTAAA GGAATGAGGA ATGGGCCCGC GCAGACTCGT 
GTTTCCTCCA GCAGCAGCCA CCAGGAGGCG ACCCTTGCCA TGGGGGACAA GGCTACAGTC 
GTGGGAGGCC AGCAGGCTGA AGCCCCAGAC TCCGTGGCCA TGTCTTCCTG GGAAAGGCGG 
CTCCATCGGG CCAAGTGTGC ACCATCCTAC TTGTTCTCCT GCTTCAATGG AGGCGAGTGT 
GTGCACCCAG CCTTCTGTGA CTGCAGACGC TTCAATGCCA CTGGACCGCG CTGCCAGATG 
GTGTACAATG CCGGCCCTGA GAGGGACAGC ATTTGCCGGG CGTGGGGGCA GCACCACGTG 
GAGACATTTG ATGGGCTCTA CTACTACCTC TCCGGAAAGG GCAGCTACAC CCTGGTGGGT 
CGCCATGAGC CCGAGGGACA GAGCTTCTCC ATCCAGGTAC ACAATGACCC GCAGTGTGGC 
TCTTCACCCT ACACCTGCTC CAGGGCTGTC AGCCTCTTCT TTGTGGGTGA GCAGGAGATC 
CATCTGGCCA AGGAGGTCAC CCATGGAGGC ATGAGGGTCC AACTGCCACA TGTCATGGGG 
AGCGCGCGTC TGCAGCAGCT TGCCGGCTAT GTCATCGTGC GGCATCAGTC AGCCTTCACA 
CTGGCCTGGG ATGGTGCCTC GGCTGTCTAC ATCAAGATGA GTCCAGAGCT TCTGGGCTGG 
ACCCATGGGC TGTGTGGGAA CAACAATGCT GACCCCAAGG ATGATCTGGT GACCAGCTCT 
GGGAAGCTGA CTGACGACGT GGTTGAGTTT GTGCACAGCT GGCAGGAGCA GGCCCCTAAC 
CAGCCTCCAG GGCCCACAAC TTCCTCCCTG CCTCGCCCAC CGTGCCTACA GCAGAACCCA 
GGAACCATGC AGGGCGTGTA CGAGCAGTGT GAGGCTCTAC TGCGGCCCCC CTTTGACGCC 
TGCCACGCCT ACGTCAGCCC TCTGCCCTTC ACAGCCAGTT GTACCAGTGA TCTCTGCCAA 
TCAATGGGTG ATGTAGCCAC CTGGTGCCGG GCACTGGCGG AGTATGCCCG GGCGTGTGCC 
CAGGCAGGGC GGCCCTTGCA AGGCTGGAGG ACCCAGCTCC GGCAATGCAC TGTGCACTGC 
AAGGAGAAGG CCTTTACCTA CAATGAGTGC ATCGCCTGCT GCCCTGCCTC CTGCCATCCC 
CGGGCATCCT GTGTGGACAG TGAGATCGCC TGTGTGGACG GCTGCTATTG CCCCAATGGG 
CTCATCTTCG AGGATGGGGG CTGCGTGGCA CCAGCTGAGT GTCCCTGTGA GTTTCACGGG 
ACTCTGTACC CACCTGGCTC TGTGGTGAAG GAAGACTGCA ATACTTGCAC ATGCACCTCA 
GGCAAGTGGG AGTGCAGCAC AGCTGTCTGC CCAGCTGAGT GCTCAGTGAC TGGTGACATT 
CACTTCACAA CCTTTGATGG CCGCCGGTAC ACGTTCCCCG CCACATGTCA GTACATCCTG 
GCCAAGAGCC GCTCTTCGGG CACCTTCACC GTGACATTGC AGAATGCCCC ATGTGGCCTG 
AACCAAGATG GAGCCTGTGT CCAGTCAGTG TCAGTGATTC TGCACCAGGA CCCTCGGAGG 
CAGGTGACCC TGACCCAGGC AGGGGATGTC CTTCTGTTTG ACCAGTACAA GATCATCCCG 
CCATACACAG ATGATGCCTT TGAGATCCGT AGGCTGTCCT CCGTGTTCCT GCGGGTGAGG 
ACGAACGTGG GCGTGCGGGT GCTCTACGAC CGTGAAGGGC TCCGACTGTA CCTGCAAGTG 
GACCAGCGAT GGGTGGAGGA TACCGTGGGC CTCTGCGGCA CCTTCAATGG CAACACGCAG 
GATGACTTCC TGTCTCCAGT GGGTGTACCT GAGAGCACCC CACAACTTTT TGGCAATTCC 
TGGAAAACAC TTTCTGCTTG CTCCCCGCTG GTCTCTGGCT CCCCTCTGGA CCCCTGCGAT 
GTGCACCTGC AAGCCGCCTC CTACTCAGTG CAGGCCTGCA GCGTGCTCAC GGGGGAGATG 
TTTGCGCCCT GCTCTGCGTT CCTGAGCCCC GTGCCCTACT TTGAGCAGTG CCGCAGGGAT 
GCCTGCCGCT GCGGGCAGCC CTGCCTGTGC GCCACACTGG CCCACTACGC CCACCTGTGC 
CGGCGCCATG GGCTCCCCGT TGATTTCCGC GCCCGCCTGC CAGCCTGTGC ACTGTCCTGT 
GAGGCCTCCA AGGAGTATAG CCCCTGCGTG GCCCCGTGTG GACGTACCTG CCAGGACCTG 
GCCAGCCCTG AGGCCTGTGG GGTTGATGGT GGCGATGACC TGAGCAGAGA CGAGTGTGTG 
GAGGGCTGTG CCTGCCCACC GGACACCTAT CTGGACACCC AGGCTGACCT CTGTGTCCCC 
CGGAACCAGT GCTCCTGCCA CTTCCAGGGA GTGGACTATC CCCCCGGAGA CAGTGACATC 
CCATCCCTGG GCCACTGCCA CTGCAAAGAT GGAGTCATGA GCTGTGATAG CAGAGCCCCA 
GCTGCTGCCT GCCCAGCAGG CCAGGTCTTC GTGAACTGCA GCGACCTGCA CACGGACCTG 
GAGCTGAGCA GGGAGAGGAC GTGTGAGCAG CAACTGCTGA ACCTGAGCGT GTCAGCCCGT 
GGCCCCTGCC TCTCGGGCTG CGCCTGTCCC CAGGGTCTGC TCAGACACGG GGATGCATGT 
TTCCTGCCAG AGGAGTGCCC CTGCACTTGG AAGGGGAAGG AGTATTTCCC TGGGGACCAG 
GTGATGTCTC CTTGCCATAC CTGTGTGTGC CAGCGGGGCT CATTCCAGTG CACCCTGCAC 
CCTTGCGCCT CCACCTGCAC TGCCTATGGG GACCGGCATT ACCGCACGTT TGATGGGCTC 
CCGTTTGACT TCGTGGGGGC ATGCAAAGTG CACCTGGTCA AGAGCACATC AGATGTCAGC 
TTCTCTGTGA TTGTAGAGAA TGTGAACTGC TACAGCTCTG GCATGATCTG CAGGAAATTT 
ATTTCCATCA ACGTTGGGAA CTCACTCATT GTCTTTGATG ATGACTCCGG AAATCCTAGT 
CCAGAGAGCT TCCTGGATGA CAAGCAGGAG GTCCACACAT GGCGAGTGGG ATTTTTCACA 
CTGGTGCATT TCCCACAGGA GCACATCACC CTCTTGTGGG ACCAGAGAAC CACAGTGCAC 
GTCCAGGCTG GGCCTCAGTG GCAGGGCCAG CTGGCGGGCC TCTGTGGGAA CTTTGACTTA 
AAAACCATCA ATGAGATGAG GACCCCGGAG AACCTAGAGC TAACTAACCC CCAGGAGTTT 
GGCAGCAGTT GGGCTGCAGT TGAGTGCCCA GACACCCTCG ATCCTCGGGA TATGTGTGTC 
CTGAATCCTC TCCGAGAACC ATTTGCCAAG AAGGAGTGCA GCATCCTGCT CAGTGAGGTG 
TTTGAGATCT GCCACCCTGT GGTTGATGTC ACTTGGTTTT ACTCAAACTG CCTGACAGAC 
ACATGTGGCT GCAGCCAGGG TGGTGACTGT GAGTGCTTCT GTGCCAGCGT CTCCGCTTAT 
GCCCACCAGT GTTGCCAGCA TGGGGTGGCT GTTGACTGGC GAACCCCCCG CCTCTGCCCG 
TATGACTGTG ACTTCTTTAA CAAAGTGCTA GGTAAGGGCC CCTATCAGCT ATCCAGCTTG 
GCAGCCGGTG GTGCTCTGGT GGGCATGAAG GCGGTGGGCG ATGACATAGT CCTAGTGAGG 
ACAGAGGATG TGGCGCCAGC AGACATTGTG AGCTTCCTGC TGACAGCTGC TCTGTACAAG 
GCCAAGGCCC ATGACCCAGA TGTGGTGTCC CTGGAGGCAG CAGACAGACC CAACTTCTTC 
CTTCACGTCA CAGCCAACGG GTCTCTGGAG CTGGCTAAGT GGCAGGGCCG TGACACCTTC 
CAACAGCATG CCTCCTTCTT GCTGCACCGG GGGACACGGC AGGCAGGCCT GGTGGCCCTG 
GAGTCCCTGG CCAAGCCCAG CTCCTTCCTC TATGTGTCGG GCGCGGTGCT GGCCCTGCGG 
CTGTACGAAC ACACAGAGGT GTTCCGCCGG GGCACACTCT TCCGCCTTCT GGATGCCAAG 
CCCTCGGGGG CTGCCTACCC CATCTGCGAG TGGCGCTACG ATGCCTGTGC CAGCCCCTGC 
TTCCAAACCT GCCGGGACCC ACGGGCAGCC AGCTGCCGGG ACGTACCCAG GGTAGAAGGC 
TGTGTCCCTG TGTGCCCCAC CCCCCAGGTC CTGGATGAAG TCACACAGAG ATGTGTCTAC 
TTGGAGGACT GTGTGGAGCC AGCAGTTTGG GTTCCCACAG AGGCCCTTGG CAATGAGACC 
CTCCCTCCCA GTCAAGGGTT GCCCACTCCC AGTGATGAGG AGCCACAGCT GTCACAGGAA 
AGCCCCAGGA CCCCCACCCA CAGGCCAGCC CTCACCCCAG CTGCCCCACT CACCACAGCC 
CTGAACCCAC CAGTGACAGC CACTGAGGAG CCAGTGGTGT CTCCAGGCCC CACCCAGACC 
ACCCTGCAGC AGCCACTGGA GCTCACTGCA TCTCAACTCC CCGCCGGCCC CACGGAGTCC 
CCAGCCAGCA AGGGAGTGAC TGCCAGCCTC CTGGCCATCC CCCATACACC AGAGTCCTCA 
TCCCTCCCTG TTGCACTGCA GACACCCACA CCTGGCATGG TGTCAGGTGC CATGGAGACA 
ACAAGGGTGA CTGTGATCTT TGCAGGAAGC CCTAACATCA CAGTCTCCTC CCGGTCGCCC 
CCTGCCCCTC GCTTCCCGCT CATGACCAAG GCTGTGACAG TCCGAGGCCA TGGCTCCTTG 
CCTGTTAGGA CGACACCCCC ACAGCCCTCC TTGACAGCAA GTCCCTCCTC CAGACCTGTG 
GCTTCCCCTG GAGCCATCTC CAGGTCCCCC ACCTCCTCGG GATCCCACAA GGCTGTGCTG 
ACACCTGCAG TAACTAAGGT CATAAGCAGG ACAGGGGTCC CCCAGCCCAC CCAGGCCCAG 
AGTGCTTCAA GTCCCAGCAC CCCTCTAACT GTGGCTGGAA CAGCAGCAGA ACAGGTTCCT 
GTCAGTCCCC TTGCAACCAG GAGCTTGGAG ATAGTGCTAT CCACAGAGAA GGGCGAAGCC 
GGGCACAGCC AGCCCATGGG CTCGCCTGCC TCCCCACAGC CACACCCACT CCCCTCTGCA 
CCACCCCGCC CAGCCCAGCA TACCACCATG GCCACCAGGT CTCCAGCTCT GCCCCCAGAG 
ACCCCAGCTG CCGCCAGCCT GTCAACAGCC ACTGATGGGC TGGCAGCCAC ACCCTTCATG 
TCCCTTGAGT CAACTCGTCC CTCCCAGCTC CTCTCTGGCC TGCCTCCCGA CACCAGCCTG 
CCCCTGGCCA AGGTGGGCAC ATCTGCCCCA GTGGCCACAC CCGGCCCCAA AGCCTCTGTC 
ATCACCACTC CACTCCAGCC ACAGGCCACG ACTCTGCCTG CTCAGACACT TAGCCCAGTA 
CTGCCTTTCA CTCCAGCAGC AATGACCCAG GCGCACCCAC CCACTCACAT AGCACCCCCA 
GCAGCAGGCA CAGCTCCAGG CCTGCTGCTG GGAGCCACAT TGCCAACCTC TGGAGTCCTG 
CCTGTGGCTG AGGGCACGGC CTCCATGGTA TCTGTTGTCC CACGAAAGAG CACCACAGGG 
AAGGTGGCCA TCCTATCCAA GCAAGTGTCT CTGCCCACTT CCATGTATGG TTCTGCAGAG 
GGTGGGCCCA CAGAGCTCAC GCCTGCTACG AGCCACCCTC TCACGCCCTT GGTGGCTGAG 
CCCGAGGGAG CCCAGGCAGG CACAGCTCTG CCAGTGCCCA CATCCTATGC CCTGAGCCGT 
GTCTCAGCCA GGACGGCCCC CCAAGACAGC ATGCTGGTTC TGTTGCCTCA GCTGGCTGAG 
GCCCATGGAA CCTCGGCAGG GCCTCACCTG GCAGCAGAGC CGGTGGACGA GGCCACCACA 
GAACCATCTG GGCGCTCAGC CCCAGCCCTG AGCATCGTAG AGGGTTTGGC GGAGGCTTTG 
GCAACTACCA CTGAGGCCAA TACATCCACC ACCTGTGTTC CAATCGCCGA GCAGGACTGC 
GTCCGCCACA TCTGCCTGGA GGGCCAGCTG ATTCGCGTGA ATCAGTCCCA GCACTGTCCC 
CAGGGTGCTG CTCCCCCTCG CTGTGGGATC CTGGGCCTCG CCGTGCGGGT GGGTGGGGAC 
CGCTGCTGCC CACTCTGGGA GTGTGCCTGC CGGTGCTCAA TCTTCCCTGA CCTGAGCTTC 
GTGACCTTCG ATGGGAGCCA CGTAGCTCTG TTCAAGGAGG CCATCTACAT CCTCAGCCAG 
AGCCCAGATG AAATGCTCAC CGTCCATGTA CTGGACTGCA AAAGTGCCAA CCTGGGGCAC 
CTGAACTGGC CCCCGTTCTG TCTGGTGATG TTGAACATGA CTCACTTGGC CCATCAGGTC 
ACTATTGATC GCTTCAACCG AAAGGTGACT GTGGACTTGC AGCCTGTGTG GCCACCGGTG 
AGCAGGTATG GATTCAGAAT TGAGGACACA GGCCACATGT ACATGATCCT GACTCCCTCA 
GACATCCAGA TCCAGTGGCT CCACAGCTCA GGACTCATGA TCGTGGAGGC CAGCAAAACC 
AGCAAGGCCC AGGGCCATGG CCTGTGCGGT ATCTGTGATG GAGATGCAGC CAATGACCTT 
ACCCTGAAGG ATGGCTCAGT GGTGGGTGGG GCTGAGGACC CTGCTCCCTT TCTGGACAGC 
TGGCAGGTGC CCAGCTCCCT GACCTCAGTG GGCCAGACCC GCTTCCGCCC AGACAGCTGC 
GCCACAACTG ACTGCTCGCC CTGCCTTCGC ATGGTGTCCA ACCGCACCTT CAGTGCCTGC 
CACCGCTTTG TGCCTCCGGA GTCATTCTGT GAGCTGTGGA TCCGGGACAC CAAGTACGTG 
CAGCAGCCCT GCGTGGCCCT GACTGTGTAC GTGGCCATGT GCCACAAATT TCATGTGTGC 
ATCGAGTGGC GGCGCTCTGA CTACTGCCCC TTCCTGTGCT CCAGCGACTC CACATACCAG 
GCATGTGTGA CAGCCTGTGA GCCACCCAAG ACATGCCAGG ATGGGATACT AGGGCCTCTG 
GACCCAGAGC ACTGCCAGGT GCTGGGCGAG GGCTGCGTCT GCTCCGAGGG CACCATCTTA 
CACCGGCGCC ACTCTGCACT CTGCATCCCG GAGGCCAAGT GCGCCTGCAC TGACAGCATG 
GGGGTGCCGA GGGCCCTGGG GGAGACCTGG AACAGCTCCC TCAGCGGCTG CTGCCAGCAC 
CAGTGCCAAG CCCCAGACAC CATTGTCCCG GTGGATCTGG GCTGCCCCAG TCCCCGCCCT 
GAGAGCTGCC TGCGATTCGG GGAGGTGGCC TTGCTCCTAC CCACCAAGGA CCCCTGCTGC 
CTGGGGACTG TCTGTGTGTG TAACCAGACT CTGTGTGAGG GTCTCGCCCC CACATGCCGC 
CCAGGCCACC GCCTCCTCAC CCACTTCCAG GAGGACTCCT GCTGCCCCAG CTACAGCTGT 
GAGTGTGACC CAGATCTCTG TGAGGCAGAG CTGGTCCCCA GCTGCCGACA GGACCAGATC 
CTGATCACGG GCCGCCTGGG GGACTCCTGC TGCACCTCCT ACTTCTGCGC CTGTGGTGAC 
TGTCCAGACT CCATCCCCGA ATGTCAAGAA GGGGAGGCGC TCACTGTGCA CAGGAATACC 
ACGGAACTCT GCTGCCCTCT GTACCAGTGT GTGTGTGAGA ACTTCCGCTG TCCCCAAGTG 
CAGTGTGGCC TGGGCACTGC CCTGGTGGAG GTGTGGAGCC CCGACCGCTG CTGCCCCTAC 
AAATCCTGTG AATGTGACTG TGACACAATC CCGGTGCCCC GGTGCCATCT GTGGGAGAAA 
TCCCAGCTGG ATGAGGAGTT CATGCACAGC GTGGAGAATG TGTGTGGCTG CGCCAAGTAC 
GAGTGTGTGA AGGCCCCGGT GTGTCTGAGC CGCGAGCTGG GTGTGATGCA GCCCGGCCAG 
ACAGTGGTGG AGCTCTCAGC AGATGGCGTG TGCCACACCT CCCGCTGCAC CACCGTGCTC 
GACCCTCTCA CCAACTTCTA CCAGATCAAC ACCACCTCCG TGCTCTGTGA CATCCACTGT 
GAGGCGAACC AGGAGTACGA GCACCCGCGG GACCTCGCTG CCTGCTGCGG CTCCTGCAGG 
AACGTGTCCT GTCTCTTCAC CTTCCCCAAT GGCACCACCT CCCTGTTCTT GCCCGGGGCA 
TCCTGGATCG CAGACTGCGC CCGCCACCAC TGCAGCAGCA CGCCCCTGGG TGCCGTGCTG 
GTCCGCTCTC CCATAAGCTG CCCACCGCTC AATGAGACTG AGTGTGCCAA GGTTGGGGGT 
TCCGTGGTAC CTTCCTTGGA AGGATGCTGC AGGACCTGTA AGGAGGATGG GCGCTCCTGC 
AAGAAGGTGA CCATCCGCAT GACCATCCGC AAGAATGAAT GCAGGAGCAG CACCCCTGTG 
AACCTAGTGT CCTGCGATGG GAGGTGCCCA TCCGCCAGCA TCTACAACTA CAACATCAAC 
ACCTATGCCC GATTCTGCAA GTGCTGCCGT GAGGTGGGCC TGCAGCGGCG CTCTGTGCAG 
CTCTTCTGTG CCACCAATGC CACCTGGGTG CCCTATACAG TGCAGGAGCC CACCGACTGT 
GCCTGCCAGT GGTCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Otolith tethering in the zebrafish otic vesicle requires Otogelin and ?-Tectorin.
Development (Cambridge, England)142(6)1137-45(2015 Mar)
Stooke-Vaughan GA,Obholzer ND,Baxendale S,Megason SG,Whitfield TT

Similar phenotypes caused by mutations in OTOG and OTOGL.
Ear and hearing35(3)e84-91(2014 May-Jun)
Oonk AM,Leijendeckers JM,Huygen PL,Schraders M,del Campo M,del Castillo I,Tekin M,Feenstra I,Beynon AJ,Kunst HP,Snik AF,Kremer H,Admiraal RJ,Pennings RJ

A phylomedicine approach to understanding the evolution of auditory sensory perception and disease in mammals.
Evolutionary applications6(3)412-22(2013 Apr)
Kirwan JD,Bekaert M,Commins JM,Davies KT,Rossiter SJ,Teeling EC

Mutations of the gene encoding otogelin are a cause of autosomal-recessive nonsyndromic moderate hearing impairment.
American journal of human genetics91(5)883-9(2012 11)
Schraders M,Ruiz-Palmero L,Kalay E,Oostrik J,del Castillo FJ,Sezgin O,Beynon AJ,Strom TM,Pennings RJ,Zazo Seco C,Oonk AM,Kunst HP,Dom?nguez-Ruiz M,Garc?a-Arumi AM,del Campo M,Villamar M,Hoefsloot LH,Moreno F,Admiraal RJ,del Castillo I,Kremer H

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OTOG cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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