ACADM cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACADM
Entrez Gene ID	34
Full Name	acyl-CoA dehydrogenase medium chain
Synonyms	ACAD1,MCAD,MCADH
General protein information	Preferred Names acyl-CoA dehydrogenase medium chain Names medium-chain specific acyl-CoA dehydrogenase, mitochondrial acyl-CoA dehydrogenase, C-4 to C-12 straight chain acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain medium-chain acyl-CoA dehydrogenase testicular tissue protein Li 7
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p31.1
Summary	This gene encodes the medium-chain specific (C4 to C12 straight chain) acyl-Coenzyme A dehydrogenase. The homotetramer enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Defects in this gene cause medium-chain acyl-CoA dehydrogenase deficiency, a disease characterized by hepatic dysfunction, fasting hypoglycemia, and encephalopathy, which can result in infantile death. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	607008
Disorder Html:	Acyl-CoA dehydrogenase, medium chain, deficiency of, 201450 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001286042.1	NP_001272971.1	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform c
NM_001286044.1	NP_001272973.1	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform e
NM_000016.5	NP_000007.1	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a precursor
NM_001286043.1	NP_001272972.1	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform d precursor
NM_001127328.2	NP_001120800.1	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform b precursor
NM_000016.6	NP_000007.1	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	ACADM	NP_000007.1
Bos taurus (cattle)	ACADM	NP_001068703.1
Rattus norvegicus (Norway rat)	Acadm	NP_058682.2
	ACADM	XP_001101274.1
Drosophila melanogaster (fruit fly)	CG12262	NP_648149.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005662	XP_315683.2
Pan troglodytes (chimpanzee)	ACADM	NP_001104286.1
Canis lupus familiaris (dog)	ACADM	XP_005622188.1
Caenorhabditis elegans (roundworm)	acdh-8	NP_495142.1
Mus musculus (house mouse)	Acadm	NP_031408.1
Danio rerio (zebrafish)	acadm	NP_998175.2
Caenorhabditis elegans (roundworm)	acdh-7	NP_510789.1
Xenopus tropicalis (tropical clawed frog)	acadm	XP_002936129.2

Gene Ontology
Bibliography

Related articles in PubMed

Unveiling the Pathogenic Molecular Mechanisms of the Most Common Variant (p.K329E) in Medium-Chain Acyl-CoA Dehydrogenase Deficiency by in Vitro and in Silico Approaches.
Bonito CA, Nunes J, Leandro J, Louro F, Leandro P, Ventura FV, Guedes RC
Biochemistry55(51)7086-7098(2016 Dec)

The epidemiology of medium chain acyl-CoA dehydrogenase deficiency: an update.
Grosse SD, Khoury MJ, Greene CL, Crider KS, Pollitt RJ
Genetics in medicine : official journal of the American College of Medical Genetics8(4)205-12(2006 Apr)

Human long chain, very long chain and medium chain acyl-CoA dehydrogenases are specific for the S-enantiomer of 2- methylpentadecanoyl-CoA.
Battaile KP, McBurney M, Van Veldhoven PP, Vockley J
Biochimica et biophysica acta1390(3)333-8(1998 Feb)

Crystal structures of the wild type and the Glu376Gly/Thr255Glu mutant of human medium-chain acyl-CoA dehydrogenase: influence of the location of the catalytic base on substrate specificity.
Lee HJ, Wang M, Paschke R, Nandy A, Ghisla S, Kim JJ
Biochemistry35(38)12412-20(1996 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

17 VUS (37%; 7 in ACADM, 9 in GALT, and 1 in PAH) were reclassified from uncertain (6 to benign or likely benign and 11 to pathogenic or likely pathogenic). We identified common types of missing information that would have helped make a definitive classification and categorized this information by ease and cost to obtain
Title: Variants of uncertain significance in newborn screening disorders: implications for large-scale genomic sequencing.

The in silico structural changes in medium-chain acyl-CoA dehydrogenase (hMCAD) p.K329E variant protein affect the disturbed oligomeric profile, thermal stability, and conformational flexibility, with respect to the wild-type.
Title: Unveiling the Pathogenic Molecular Mechanisms of the Most Common Variant (p.K329E) in Medium-Chain Acyl-CoA Dehydrogenase Deficiency by in Vitro and in Silico Approaches.

Our study has revealed the unique genetic backgrounds of MCAD deficiency among Japanese, based on the largest series of non-Caucasian cases.
Title: Screening of MCAD deficiency in Japan: 16years' experience of enzymatic and genetic evaluation.

LCHAD and MCAD are differentially expressed in maternal and fetal tissues during normal late pregnancy, which may represent a metabolic adaptation in response to physiological maternal dyslipidemia during late pregnancy.
Title: Tissue specific expression of human fatty acid oxidation enzyme genes in late pregnancy.

Subjects with neonatal symptoms, or neonatal abnormal labs, or neonatal triggers were more likely to have at least one copy of the severe c.985A>G ACADM gene mutation
Title: 221 newborn-screened neonates with medium-chain acyl-coenzyme A dehydrogenase deficiency: Findings from the Inborn Errors of Metabolism Collaborative.

The following ACADM gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACADM cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22672	NM_001286043.1 Latest version!	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu23076	NM_000016.6 Latest version!	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu22025	NM_001286044.1 Latest version!	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OHu22748	NM_001286042.1 Latest version!	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu22920	NM_001127328.2 Latest version!	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$251.30 $359.00
View Old Accession Versions >>
OHu23076	NM_000016.5	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACADM ( NM_001286043.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001272972.1 Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu22672
Clone ID Related Accession (Same CDS sequence)	NM_001286043.1
Accession Version	NM_001286043.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1365bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform d precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357314.11 and BX647734.1. Transcript Variant: This variant (5) uses an alternate in-frame exon in the central coding region compared to variant 1. The encoded isoform (d) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX647734.1, SRR1660809.121204.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2151741 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG GTCCTGAGAA GTATTTCTCG TTTTCATTGG 
AGATCACAGC ATACAAAAGC CAATCGACAA CGTGAACCAG GATTAGGATT TAGTTTTGAG 
TTCACCGAAC AGCAGAAAGA ATTTCAAGCT ACTGCTCGTA AATTTGCCAG AGAGGAAATC 
ATCCCAGTGG CTGCAGAATA TGATAAAACT GGTGAATATC CAGTCCCCCT AATTAGAAGA 
GCCTGGGAAC TTGGTTTAAT GAACACACAC ATTCCAGAGA ACTGTGACTA CAGTGTTTGC 
CCACTTTTGG AAGCTTGCAC TCTATACCTA GATGCGTTTT TCCTTCTTCT AACTGGTTCC 
AACCTTAACT TGCACCTAAA CCTTGGAGGT CTTGGACTTG GAACTTTTGA TGCTTGTTTA 
ATTAGTGAAG AATTGGCTTA TGGATGTACA GGGGTTCAGA CTGCTATTGA AGGAAATTCT 
TTGGGGCAAA TGCCTATTAT TATTGCTGGA AATGATCAAC AAAAGAAGAA GTATTTGGGG 
AGAATGACTG AGGAGCCATT GATGTGTGCT TATTGTGTAA CAGAACCTGG AGCAGGCTCT 
GATGTAGCTG GTATAAAGAC CAAAGCAGAA AAGAAAGGAG ATGAGTATAT TATTAATGGT 
CAGAAGATGT GGATAACCAA CGGAGGAAAA GCTAATTGGT ATTTTTTATT GGCACGTTCT 
GATCCAGATC CTAAAGCTCC TGCTAATAAA GCCTTTACTG GATTCATTGT GGAAGCAGAT 
ACCCCAGGAA TTCAGATTGG GAGAAAGGAA TTAAACATGG GCCAGCGATG TTCAGATACT 
AGAGGAATTG TCTTCGAAGA TGTGAAAGTG CCTAAAGAAA ATGTTTTAAT TGGTGACGGA 
GCTGGTTTCA AAGTTGCAAT GGGAGCTTTT GATAAAACCA GACCTGTAGT AGCTGCTGGT 
GCTGTTGGAT TAGCACAAAG AGCTTTGGAT GAAGCTACCA AGTATGCCCT GGAAAGGAAA 
ACTTTCGGAA AGCTACTTGT AGAGCACCAA GCAATATCAT TTATGCTGGC TGAAATGGCA 
ATGAAAGTTG AACTAGCTAG AATGAGTTAC CAGAGAGCAG CTTGGGAGGT TGATTCTGGT 
CGTCGAAATA CCTATTATGC TTCTATTGCA AAGGCATTTG CTGGAGATAT TGCAAATCAG 
TTAGCTACTG ATGCTGTGCA GATACTTGGA GGCAATGGAT TTAATACAGA ATATCCTGTA 
GAAAAACTAA TGAGGGATGC CAAAATCTAT CAGATTTATG AAGGTACTTC ACAAATTCAA 
AGACTTATTG TAGCCCGTGA ACACATTGAC AAGTACAAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272972.1
CDS	442..1806
Translation	MAAGFGRCCRVLRSISRFHWRSQHTKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCDYSVCPLLEACTLYLDAFFLLLTGSNLNLHLNLGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Target ORF information:

RefSeq Version	NM_001286043.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286043.1

ORF Insert Sequence:

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG GTCCTGAGAA GTATTTCTCG TTTTCATTGG 
AGATCACAGC ATACAAAAGC CAATCGACAA CGTGAACCAG GATTAGGATT TAGTTTTGAG 
TTCACCGAAC AGCAGAAAGA ATTTCAAGCT ACTGCTCGTA AATTTGCCAG AGAGGAAATC 
ATCCCAGTGG CTGCAGAATA TGATAAAACT GGTGAATATC CAGTCCCCCT AATTAGAAGA 
GCCTGGGAAC TTGGTTTAAT GAACACACAC ATTCCAGAGA ACTGTGACTA CAGTGTTTGC 
CCACTTTTGG AAGCTTGCAC TCTATACCTA GATGCGTTTT TCCTTCTTCT AACTGGTTCC 
AACCTTAACT TGCACCTAAA CCTTGGAGGT CTTGGACTTG GAACTTTTGA TGCTTGTTTA 
ATTAGTGAAG AATTGGCTTA TGGATGTACA GGGGTTCAGA CTGCTATTGA AGGAAATTCT 
TTGGGGCAAA TGCCTATTAT TATTGCTGGA AATGATCAAC AAAAGAAGAA GTATTTGGGG 
AGAATGACTG AGGAGCCATT GATGTGTGCT TATTGTGTAA CAGAACCTGG AGCAGGCTCT 
GATGTAGCTG GTATAAAGAC CAAAGCAGAA AAGAAAGGAG ATGAGTATAT TATTAATGGT 
CAGAAGATGT GGATAACCAA CGGAGGAAAA GCTAATTGGT ATTTTTTATT GGCACGTTCT 
GATCCAGATC CTAAAGCTCC TGCTAATAAA GCCTTTACTG GATTCATTGT GGAAGCAGAT 
ACCCCAGGAA TTCAGATTGG GAGAAAGGAA TTAAACATGG GCCAGCGATG TTCAGATACT 
AGAGGAATTG TCTTCGAAGA TGTGAAAGTG CCTAAAGAAA ATGTTTTAAT TGGTGACGGA 
GCTGGTTTCA AAGTTGCAAT GGGAGCTTTT GATAAAACCA GACCTGTAGT AGCTGCTGGT 
GCTGTTGGAT TAGCACAAAG AGCTTTGGAT GAAGCTACCA AGTATGCCCT GGAAAGGAAA 
ACTTTCGGAA AGCTACTTGT AGAGCACCAA GCAATATCAT TTATGCTGGC TGAAATGGCA 
ATGAAAGTTG AACTAGCTAG AATGAGTTAC CAGAGAGCAG CTTGGGAGGT TGATTCTGGT 
CGTCGAAATA CCTATTATGC TTCTATTGCA AAGGCATTTG CTGGAGATAT TGCAAATCAG 
TTAGCTACTG ATGCTGTGCA GATACTTGGA GGCAATGGAT TTAATACAGA ATATCCTGTA 
GAAAAACTAA TGAGGGATGC CAAAATCTAT CAGATTTATG AAGGTACTTC ACAAATTCAA 
AGACTTATTG TAGCCCGTGA ACACATTGAC AAGTACAAAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADM ( NM_000016.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_000007.1 Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23076
Clone ID Related Accession (Same CDS sequence)	NM_000016.5 , NM_000016.6
Accession Version	NM_000016.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-03
Organism	Homo sapiens(human)
Product	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357314.11 and BC005377.1. This sequence is a reference standard in the RefSeqGene project. On Oct 29, 2013 this sequence version replaced NM_000016.4. Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005320.1, SRR1660805.243867.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG GTCCTGAGAA GTATTTCTCG TTTTCATTGG 
AGATCACAGC ATACAAAAGC CAATCGACAA CGTGAACCAG GATTAGGATT TAGTTTTGAG 
TTCACCGAAC AGCAGAAAGA ATTTCAAGCT ACTGCTCGTA AATTTGCCAG AGAGGAAATC 
ATCCCAGTGG CTGCAGAATA TGATAAAACT GGTGAATATC CAGTCCCCCT AATTAGAAGA 
GCCTGGGAAC TTGGTTTAAT GAACACACAC ATTCCAGAGA ACTGTGGAGG TCTTGGACTT 
GGAACTTTTG ATGCTTGTTT AATTAGTGAA GAATTGGCTT ATGGATGTAC AGGGGTTCAG 
ACTGCTATTG AAGGAAATTC TTTGGGGCAA ATGCCTATTA TTATTGCTGG AAATGATCAA 
CAAAAGAAGA AGTATTTGGG GAGAATGACT GAGGAGCCAT TGATGTGTGC TTATTGTGTA 
ACAGAACCTG GAGCAGGCTC TGATGTAGCT GGTATAAAGA CCAAAGCAGA AAAGAAAGGA 
GATGAGTATA TTATTAATGG TCAGAAGATG TGGATAACCA ACGGAGGAAA AGCTAATTGG 
TATTTTTTAT TGGCACGTTC TGATCCAGAT CCTAAAGCTC CTGCTAATAA AGCCTTTACT 
GGATTCATTG TGGAAGCAGA TACCCCAGGA ATTCAGATTG GGAGAAAGGA ATTAAACATG 
GGCCAGCGAT GTTCAGATAC TAGAGGAATT GTCTTCGAAG ATGTGAAAGT GCCTAAAGAA 
AATGTTTTAA TTGGTGACGG AGCTGGTTTC AAAGTTGCAA TGGGAGCTTT TGATAAAACC 
AGACCTGTAG TAGCTGCTGG TGCTGTTGGA TTAGCACAAA GAGCTTTGGA TGAAGCTACC 
AAGTATGCCC TGGAAAGGAA AACTTTCGGA AAGCTACTTG TAGAGCACCA AGCAATATCA 
TTTATGCTGG CTGAAATGGC AATGAAAGTT GAACTAGCTA GAATGAGTTA CCAGAGAGCA 
GCTTGGGAGG TTGATTCTGG TCGTCGAAAT ACCTATTATG CTTCTATTGC AAAGGCATTT 
GCTGGAGATA TTGCAAATCA GTTAGCTACT GATGCTGTGC AGATACTTGG AGGCAATGGA 
TTTAATACAG AATATCCTGT AGAAAAACTA ATGAGGGATG CCAAAATCTA TCAGATTTAT 
GAAGGTACTT CACAAATTCA AAGACTTATT GTAGCCCGTG AACACATTGA CAAGTACAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000007.1
CDS	442..1707
Translation	MAAGFGRCCRVLRSISRFHWRSQHTKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Target ORF information:

RefSeq Version	NM_000016.5
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000016.5

ORF Insert Sequence:

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG GTCCTGAGAA GTATTTCTCG TTTTCATTGG 
AGATCACAGC ATACAAAAGC CAATCGACAA CGTGAACCAG GATTAGGATT TAGTTTTGAG 
TTCACCGAAC AGCAGAAAGA ATTTCAAGCT ACTGCTCGTA AATTTGCCAG AGAGGAAATC 
ATCCCAGTGG CTGCAGAATA TGATAAAACT GGTGAATATC CAGTCCCCCT AATTAGAAGA 
GCCTGGGAAC TTGGTTTAAT GAACACACAC ATTCCAGAGA ACTGTGGAGG TCTTGGACTT 
GGAACTTTTG ATGCTTGTTT AATTAGTGAA GAATTGGCTT ATGGATGTAC AGGGGTTCAG 
ACTGCTATTG AAGGAAATTC TTTGGGGCAA ATGCCTATTA TTATTGCTGG AAATGATCAA 
CAAAAGAAGA AGTATTTGGG GAGAATGACT GAGGAGCCAT TGATGTGTGC TTATTGTGTA 
ACAGAACCTG GAGCAGGCTC TGATGTAGCT GGTATAAAGA CCAAAGCAGA AAAGAAAGGA 
GATGAGTATA TTATTAATGG TCAGAAGATG TGGATAACCA ACGGAGGAAA AGCTAATTGG 
TATTTTTTAT TGGCACGTTC TGATCCAGAT CCTAAAGCTC CTGCTAATAA AGCCTTTACT 
GGATTCATTG TGGAAGCAGA TACCCCAGGA ATTCAGATTG GGAGAAAGGA ATTAAACATG 
GGCCAGCGAT GTTCAGATAC TAGAGGAATT GTCTTCGAAG ATGTGAAAGT GCCTAAAGAA 
AATGTTTTAA TTGGTGACGG AGCTGGTTTC AAAGTTGCAA TGGGAGCTTT TGATAAAACC 
AGACCTGTAG TAGCTGCTGG TGCTGTTGGA TTAGCACAAA GAGCTTTGGA TGAAGCTACC 
AAGTATGCCC TGGAAAGGAA AACTTTCGGA AAGCTACTTG TAGAGCACCA AGCAATATCA 
TTTATGCTGG CTGAAATGGC AATGAAAGTT GAACTAGCTA GAATGAGTTA CCAGAGAGCA 
GCTTGGGAGG TTGATTCTGG TCGTCGAAAT ACCTATTATG CTTCTATTGC AAAGGCATTT 
GCTGGAGATA TTGCAAATCA GTTAGCTACT GATGCTGTGC AGATACTTGG AGGCAATGGA 
TTTAATACAG AATATCCTGT AGAAAAACTA ATGAGGGATG CCAAAATCTA TCAGATTTAT 
GAAGGTACTT CACAAATTCA AAGACTTATT GTAGCCCGTG AACACATTGA CAAGTACAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADM ( NM_000016.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_000007.1 Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23076
Clone ID Related Accession (Same CDS sequence)	NM_000016.5 , NM_000016.6
Accession Version	NM_000016.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357314.11 and BC005377.1. On Dec 4, 2019 this sequence version replaced NM_000016.5. Transcript Variant: This variant (1) encodes isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HM005320.1, BC005377.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG GTCCTGAGAA GTATTTCTCG TTTTCATTGG 
AGATCACAGC ATACAAAAGC CAATCGACAA CGTGAACCAG GATTAGGATT TAGTTTTGAG 
TTCACCGAAC AGCAGAAAGA ATTTCAAGCT ACTGCTCGTA AATTTGCCAG AGAGGAAATC 
ATCCCAGTGG CTGCAGAATA TGATAAAACT GGTGAATATC CAGTCCCCCT AATTAGAAGA 
GCCTGGGAAC TTGGTTTAAT GAACACACAC ATTCCAGAGA ACTGTGGAGG TCTTGGACTT 
GGAACTTTTG ATGCTTGTTT AATTAGTGAA GAATTGGCTT ATGGATGTAC AGGGGTTCAG 
ACTGCTATTG AAGGAAATTC TTTGGGGCAA ATGCCTATTA TTATTGCTGG AAATGATCAA 
CAAAAGAAGA AGTATTTGGG GAGAATGACT GAGGAGCCAT TGATGTGTGC TTATTGTGTA 
ACAGAACCTG GAGCAGGCTC TGATGTAGCT GGTATAAAGA CCAAAGCAGA AAAGAAAGGA 
GATGAGTATA TTATTAATGG TCAGAAGATG TGGATAACCA ACGGAGGAAA AGCTAATTGG 
TATTTTTTAT TGGCACGTTC TGATCCAGAT CCTAAAGCTC CTGCTAATAA AGCCTTTACT 
GGATTCATTG TGGAAGCAGA TACCCCAGGA ATTCAGATTG GGAGAAAGGA ATTAAACATG 
GGCCAGCGAT GTTCAGATAC TAGAGGAATT GTCTTCGAAG ATGTGAAAGT GCCTAAAGAA 
AATGTTTTAA TTGGTGACGG AGCTGGTTTC AAAGTTGCAA TGGGAGCTTT TGATAAAACC 
AGACCTGTAG TAGCTGCTGG TGCTGTTGGA TTAGCACAAA GAGCTTTGGA TGAAGCTACC 
AAGTATGCCC TGGAAAGGAA AACTTTCGGA AAGCTACTTG TAGAGCACCA AGCAATATCA 
TTTATGCTGG CTGAAATGGC AATGAAAGTT GAACTAGCTA GAATGAGTTA CCAGAGAGCA 
GCTTGGGAGG TTGATTCTGG TCGTCGAAAT ACCTATTATG CTTCTATTGC AAAGGCATTT 
GCTGGAGATA TTGCAAATCA GTTAGCTACT GATGCTGTGC AGATACTTGG AGGCAATGGA 
TTTAATACAG AATATCCTGT AGAAAAACTA ATGAGGGATG CCAAAATCTA TCAGATTTAT 
GAAGGTACTT CACAAATTCA AAGACTTATT GTAGCCCGTG AACACATTGA CAAGTACAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000007.1
CDS	80..1345
Translation	MAAGFGRCCRVLRSISRFHWRSQHTKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Target ORF information:

RefSeq Version	NM_000016.6
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000016.6

ORF Insert Sequence:

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG GTCCTGAGAA GTATTTCTCG TTTTCATTGG 
AGATCACAGC ATACAAAAGC CAATCGACAA CGTGAACCAG GATTAGGATT TAGTTTTGAG 
TTCACCGAAC AGCAGAAAGA ATTTCAAGCT ACTGCTCGTA AATTTGCCAG AGAGGAAATC 
ATCCCAGTGG CTGCAGAATA TGATAAAACT GGTGAATATC CAGTCCCCCT AATTAGAAGA 
GCCTGGGAAC TTGGTTTAAT GAACACACAC ATTCCAGAGA ACTGTGGAGG TCTTGGACTT 
GGAACTTTTG ATGCTTGTTT AATTAGTGAA GAATTGGCTT ATGGATGTAC AGGGGTTCAG 
ACTGCTATTG AAGGAAATTC TTTGGGGCAA ATGCCTATTA TTATTGCTGG AAATGATCAA 
CAAAAGAAGA AGTATTTGGG GAGAATGACT GAGGAGCCAT TGATGTGTGC TTATTGTGTA 
ACAGAACCTG GAGCAGGCTC TGATGTAGCT GGTATAAAGA CCAAAGCAGA AAAGAAAGGA 
GATGAGTATA TTATTAATGG TCAGAAGATG TGGATAACCA ACGGAGGAAA AGCTAATTGG 
TATTTTTTAT TGGCACGTTC TGATCCAGAT CCTAAAGCTC CTGCTAATAA AGCCTTTACT 
GGATTCATTG TGGAAGCAGA TACCCCAGGA ATTCAGATTG GGAGAAAGGA ATTAAACATG 
GGCCAGCGAT GTTCAGATAC TAGAGGAATT GTCTTCGAAG ATGTGAAAGT GCCTAAAGAA 
AATGTTTTAA TTGGTGACGG AGCTGGTTTC AAAGTTGCAA TGGGAGCTTT TGATAAAACC 
AGACCTGTAG TAGCTGCTGG TGCTGTTGGA TTAGCACAAA GAGCTTTGGA TGAAGCTACC 
AAGTATGCCC TGGAAAGGAA AACTTTCGGA AAGCTACTTG TAGAGCACCA AGCAATATCA 
TTTATGCTGG CTGAAATGGC AATGAAAGTT GAACTAGCTA GAATGAGTTA CCAGAGAGCA 
GCTTGGGAGG TTGATTCTGG TCGTCGAAAT ACCTATTATG CTTCTATTGC AAAGGCATTT 
GCTGGAGATA TTGCAAATCA GTTAGCTACT GATGCTGTGC AGATACTTGG AGGCAATGGA 
TTTAATACAG AATATCCTGT AGAAAAACTA ATGAGGGATG CCAAAATCTA TCAGATTTAT 
GAAGGTACTT CACAAATTCA AAGACTTATT GTAGCCCGTG AACACATTGA CAAGTACAAA 
AATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADM ( NM_001286044.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001272973.1 Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 6, mRNA.

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CloneID	OHu22025
Clone ID Related Accession (Same CDS sequence)	NM_001286044.1
Accession Version	NM_001286044.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 699bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357314.11 and AK296045.1. Transcript Variant: This variant (6) uses an alternate 5' structure that results in the use downstream AUG compared to variant 1. The encoded isoform (e) has a shorter N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.214363.1, AK296045.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGTGGATAA CCAACGGAGG AAAAGCTAAT TGGTATTTTT TATTGGCACG TTCTGATCCA 
GATCCTAAAG CTCCTGCTAA TAAAGCCTTT ACTGGATTCA TTGTGGAAGC AGATACCCCA 
GGAATTCAGA TTGGGAGAAA GGAATTAAAC ATGGGCCAGC GATGTTCAGA TACTAGAGGA 
ATTGTCTTCG AAGATGTGAA AGTGCCTAAA GAAAATGTTT TAATTGGTGA CGGAGCTGGT 
TTCAAAGTTG CAATGGGAGC TTTTGATAAA ACCAGACCTG TAGTAGCTGC TGGTGCTGTT 
GGATTAGCAC AAAGAGCTTT GGATGAAGCT ACCAAGTATG CCCTGGAAAG GAAAACTTTC 
GGAAAGCTAC TTGTAGAGCA CCAAGCAATA TCATTTATGC TGGCTGAAAT GGCAATGAAA 
GTTGAACTAG CTAGAATGAG TTACCAGAGA GCAGCTTGGG AGGTTGATTC TGGTCGTCGA 
AATACCTATT ATGCTTCTAT TGCAAAGGCA TTTGCTGGAG ATATTGCAAA TCAGTTAGCT 
ACTGATGCTG TGCAGATACT TGGAGGCAAT GGATTTAATA CAGAATATCC TGTAGAAAAA 
CTAATGAGGG ATGCCAAAAT CTATCAGATT TATGAAGGTA CTTCACAAAT TCAAAGACTT 
ATTGTAGCCC GTGAACACAT TGACAAGTAC AAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272973.1
CDS	739..1437
Translation	MWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Target ORF information:

RefSeq Version	NM_001286044.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286044.1

ORF Insert Sequence:

ATGTGGATAA CCAACGGAGG AAAAGCTAAT TGGTATTTTT TATTGGCACG TTCTGATCCA 
GATCCTAAAG CTCCTGCTAA TAAAGCCTTT ACTGGATTCA TTGTGGAAGC AGATACCCCA 
GGAATTCAGA TTGGGAGAAA GGAATTAAAC ATGGGCCAGC GATGTTCAGA TACTAGAGGA 
ATTGTCTTCG AAGATGTGAA AGTGCCTAAA GAAAATGTTT TAATTGGTGA CGGAGCTGGT 
TTCAAAGTTG CAATGGGAGC TTTTGATAAA ACCAGACCTG TAGTAGCTGC TGGTGCTGTT 
GGATTAGCAC AAAGAGCTTT GGATGAAGCT ACCAAGTATG CCCTGGAAAG GAAAACTTTC 
GGAAAGCTAC TTGTAGAGCA CCAAGCAATA TCATTTATGC TGGCTGAAAT GGCAATGAAA 
GTTGAACTAG CTAGAATGAG TTACCAGAGA GCAGCTTGGG AGGTTGATTC TGGTCGTCGA 
AATACCTATT ATGCTTCTAT TGCAAAGGCA TTTGCTGGAG ATATTGCAAA TCAGTTAGCT 
ACTGATGCTG TGCAGATACT TGGAGGCAAT GGATTTAATA CAGAATATCC TGTAGAAAAA 
CTAATGAGGG ATGCCAAAAT CTATCAGATT TATGAAGGTA CTTCACAAAT TCAAAGACTT 
ATTGTAGCCC GTGAACACAT TGACAAGTAC AAAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADM ( NM_001286042.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001272971.1 Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 4, mRNA.

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CloneID	OHu22748
Clone ID Related Accession (Same CDS sequence)	NM_001286042.1
Accession Version	NM_001286042.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB055575.1, AL357314.11 and AK315946.1. On Oct 29, 2013 this sequence version replaced XM_005270811.1. Transcript Variant: This variant (4) differs in the 5' UTR and lacks an exon in the 5' coding region. These difference cause translation initiation at an alternate start codon compared to variant 1. The encoded isoform (c) is shorter and has a distinct N-terminus compared to isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.167612.1, SRR1660803.106415.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCTGCAGG AGTTCACCGA ACAGCAGAAA GAATTTCAAG CTACTGCTCG TAAATTTGCC 
AGAGAGGAAA TCATCCCAGT GGCTGCAGAA TATGATAAAA CTGGTGAATA TCCAGTCCCC 
CTAATTAGAA GAGCCTGGGA ACTTGGTTTA ATGAACACAC ACATTCCAGA GAACTGTGGA 
GGTCTTGGAC TTGGAACTTT TGATGCTTGT TTAATTAGTG AAGAATTGGC TTATGGATGT 
ACAGGGGTTC AGACTGCTAT TGAAGGAAAT TCTTTGGGGC AAATGCCTAT TATTATTGCT 
GGAAATGATC AACAAAAGAA GAAGTATTTG GGGAGAATGA CTGAGGAGCC ATTGATGTGT 
GCTTATTGTG TAACAGAACC TGGAGCAGGC TCTGATGTAG CTGGTATAAA GACCAAAGCA 
GAAAAGAAAG GAGATGAGTA TATTATTAAT GGTCAGAAGA TGTGGATAAC CAACGGAGGA 
AAAGCTAATT GGTATTTTTT ATTGGCACGT TCTGATCCAG ATCCTAAAGC TCCTGCTAAT 
AAAGCCTTTA CTGGATTCAT TGTGGAAGCA GATACCCCAG GAATTCAGAT TGGGAGAAAG 
GAATTAAACA TGGGCCAGCG ATGTTCAGAT ACTAGAGGAA TTGTCTTCGA AGATGTGAAA 
GTGCCTAAAG AAAATGTTTT AATTGGTGAC GGAGCTGGTT TCAAAGTTGC AATGGGAGCT 
TTTGATAAAA CCAGACCTGT AGTAGCTGCT GGTGCTGTTG GATTAGCACA AAGAGCTTTG 
GATGAAGCTA CCAAGTATGC CCTGGAAAGG AAAACTTTCG GAAAGCTACT TGTAGAGCAC 
CAAGCAATAT CATTTATGCT GGCTGAAATG GCAATGAAAG TTGAACTAGC TAGAATGAGT 
TACCAGAGAG CAGCTTGGGA GGTTGATTCT GGTCGTCGAA ATACCTATTA TGCTTCTATT 
GCAAAGGCAT TTGCTGGAGA TATTGCAAAT CAGTTAGCTA CTGATGCTGT GCAGATACTT 
GGAGGCAATG GATTTAATAC AGAATATCCT GTAGAAAAAC TAATGAGGGA TGCCAAAATC 
TATCAGATTT ATGAAGGTAC TTCACAAATT CAAAGACTTA TTGTAGCCCG TGAACACATT 
GACAAGTACA AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001272971.1
CDS	462..1619
Translation	MLQEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Target ORF information:

RefSeq Version	NM_001286042.1
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001286042.1

ORF Insert Sequence:

ATGCTGCAGG AGTTCACCGA ACAGCAGAAA GAATTTCAAG CTACTGCTCG TAAATTTGCC 
AGAGAGGAAA TCATCCCAGT GGCTGCAGAA TATGATAAAA CTGGTGAATA TCCAGTCCCC 
CTAATTAGAA GAGCCTGGGA ACTTGGTTTA ATGAACACAC ACATTCCAGA GAACTGTGGA 
GGTCTTGGAC TTGGAACTTT TGATGCTTGT TTAATTAGTG AAGAATTGGC TTATGGATGT 
ACAGGGGTTC AGACTGCTAT TGAAGGAAAT TCTTTGGGGC AAATGCCTAT TATTATTGCT 
GGAAATGATC AACAAAAGAA GAAGTATTTG GGGAGAATGA CTGAGGAGCC ATTGATGTGT 
GCTTATTGTG TAACAGAACC TGGAGCAGGC TCTGATGTAG CTGGTATAAA GACCAAAGCA 
GAAAAGAAAG GAGATGAGTA TATTATTAAT GGTCAGAAGA TGTGGATAAC CAACGGAGGA 
AAAGCTAATT GGTATTTTTT ATTGGCACGT TCTGATCCAG ATCCTAAAGC TCCTGCTAAT 
AAAGCCTTTA CTGGATTCAT TGTGGAAGCA GATACCCCAG GAATTCAGAT TGGGAGAAAG 
GAATTAAACA TGGGCCAGCG ATGTTCAGAT ACTAGAGGAA TTGTCTTCGA AGATGTGAAA 
GTGCCTAAAG AAAATGTTTT AATTGGTGAC GGAGCTGGTT TCAAAGTTGC AATGGGAGCT 
TTTGATAAAA CCAGACCTGT AGTAGCTGCT GGTGCTGTTG GATTAGCACA AAGAGCTTTG 
GATGAAGCTA CCAAGTATGC CCTGGAAAGG AAAACTTTCG GAAAGCTACT TGTAGAGCAC 
CAAGCAATAT CATTTATGCT GGCTGAAATG GCAATGAAAG TTGAACTAGC TAGAATGAGT 
TACCAGAGAG CAGCTTGGGA GGTTGATTCT GGTCGTCGAA ATACCTATTA TGCTTCTATT 
GCAAAGGCAT TTGCTGGAGA TATTGCAAAT CAGTTAGCTA CTGATGCTGT GCAGATACTT 
GGAGGCAATG GATTTAATAC AGAATATCCT GTAGAAAAAC TAATGAGGGA TGCCAAAATC 
TATCAGATTT ATGAAGGTAC TTCACAAATT CAAAGACTTA TTGTAGCCCG TGAACACATT 
GACAAGTACA AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACADM ( NM_001127328.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001120800.1 Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu22920
Clone ID Related Accession (Same CDS sequence)	NM_001127328.2
Accession Version	NM_001127328.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1278bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-15
Organism	Homo sapiens(human)
Product	medium-chain specific acyl-CoA dehydrogenase, mitochondrial isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL357314.11 and AF251043.1. On Oct 29, 2013 this sequence version replaced NM_001127328.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in an isoform (b) that is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.119181.1, SRR1660809.4230.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG TGTTCTTTAC AGGTCCTGAG AAGTATTTCT 
CGTTTTCATT GGAGATCACA GCATACAAAA GCCAATCGAC AACGTGAACC AGGATTAGGA 
TTTAGTTTTG AGTTCACCGA ACAGCAGAAA GAATTTCAAG CTACTGCTCG TAAATTTGCC 
AGAGAGGAAA TCATCCCAGT GGCTGCAGAA TATGATAAAA CTGGTGAATA TCCAGTCCCC 
CTAATTAGAA GAGCCTGGGA ACTTGGTTTA ATGAACACAC ACATTCCAGA GAACTGTGGA 
GGTCTTGGAC TTGGAACTTT TGATGCTTGT TTAATTAGTG AAGAATTGGC TTATGGATGT 
ACAGGGGTTC AGACTGCTAT TGAAGGAAAT TCTTTGGGGC AAATGCCTAT TATTATTGCT 
GGAAATGATC AACAAAAGAA GAAGTATTTG GGGAGAATGA CTGAGGAGCC ATTGATGTGT 
GCTTATTGTG TAACAGAACC TGGAGCAGGC TCTGATGTAG CTGGTATAAA GACCAAAGCA 
GAAAAGAAAG GAGATGAGTA TATTATTAAT GGTCAGAAGA TGTGGATAAC CAACGGAGGA 
AAAGCTAATT GGTATTTTTT ATTGGCACGT TCTGATCCAG ATCCTAAAGC TCCTGCTAAT 
AAAGCCTTTA CTGGATTCAT TGTGGAAGCA GATACCCCAG GAATTCAGAT TGGGAGAAAG 
GAATTAAACA TGGGCCAGCG ATGTTCAGAT ACTAGAGGAA TTGTCTTCGA AGATGTGAAA 
GTGCCTAAAG AAAATGTTTT AATTGGTGAC GGAGCTGGTT TCAAAGTTGC AATGGGAGCT 
TTTGATAAAA CCAGACCTGT AGTAGCTGCT GGTGCTGTTG GATTAGCACA AAGAGCTTTG 
GATGAAGCTA CCAAGTATGC CCTGGAAAGG AAAACTTTCG GAAAGCTACT TGTAGAGCAC 
CAAGCAATAT CATTTATGCT GGCTGAAATG GCAATGAAAG TTGAACTAGC TAGAATGAGT 
TACCAGAGAG CAGCTTGGGA GGTTGATTCT GGTCGTCGAA ATACCTATTA TGCTTCTATT 
GCAAAGGCAT TTGCTGGAGA TATTGCAAAT CAGTTAGCTA CTGATGCTGT GCAGATACTT 
GGAGGCAATG GATTTAATAC AGAATATCCT GTAGAAAAAC TAATGAGGGA TGCCAAAATC 
TATCAGATTT ATGAAGGTAC TTCACAAATT CAAAGACTTA TTGTAGCCCG TGAACACATT 
GACAAGTACA AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120800.1
CDS	442..1719
Translation	MAAGFGRCCRCSLQVLRSISRFHWRSQHTKANRQREPGLGFSFEFTEQQKEFQATARKFAREEIIPVAAEYDKTGEYPVPLIRRAWELGLMNTHIPENCGGLGLGTFDACLISEELAYGCTGVQTAIEGNSLGQMPIIIAGNDQQKKKYLGRMTEEPLMCAYCVTEPGAGSDVAGIKTKAEKKGDEYIINGQKMWITNGGKANWYFLLARSDPDPKAPANKAFTGFIVEADTPGIQIGRKELNMGQRCSDTRGIVFEDVKVPKENVLIGDGAGFKVAMGAFDKTRPVVAAGAVGLAQRALDEATKYALERKTFGKLLVEHQAISFMLAEMAMKVELARMSYQRAAWEVDSGRRNTYYASIAKAFAGDIANQLATDAVQILGGNGFNTEYPVEKLMRDAKIYQIYEGTSQIQRLIVAREHIDKYKN

Target ORF information:

RefSeq Version	NM_001127328.2
Organism	Homo sapiens(human)
Definition	Homo sapiens acyl-CoA dehydrogenase medium chain (ACADM), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127328.2

ORF Insert Sequence:

ATGGCAGCGG GGTTCGGGCG ATGCTGCAGG TGTTCTTTAC AGGTCCTGAG AAGTATTTCT 
CGTTTTCATT GGAGATCACA GCATACAAAA GCCAATCGAC AACGTGAACC AGGATTAGGA 
TTTAGTTTTG AGTTCACCGA ACAGCAGAAA GAATTTCAAG CTACTGCTCG TAAATTTGCC 
AGAGAGGAAA TCATCCCAGT GGCTGCAGAA TATGATAAAA CTGGTGAATA TCCAGTCCCC 
CTAATTAGAA GAGCCTGGGA ACTTGGTTTA ATGAACACAC ACATTCCAGA GAACTGTGGA 
GGTCTTGGAC TTGGAACTTT TGATGCTTGT TTAATTAGTG AAGAATTGGC TTATGGATGT 
ACAGGGGTTC AGACTGCTAT TGAAGGAAAT TCTTTGGGGC AAATGCCTAT TATTATTGCT 
GGAAATGATC AACAAAAGAA GAAGTATTTG GGGAGAATGA CTGAGGAGCC ATTGATGTGT 
GCTTATTGTG TAACAGAACC TGGAGCAGGC TCTGATGTAG CTGGTATAAA GACCAAAGCA 
GAAAAGAAAG GAGATGAGTA TATTATTAAT GGTCAGAAGA TGTGGATAAC CAACGGAGGA 
AAAGCTAATT GGTATTTTTT ATTGGCACGT TCTGATCCAG ATCCTAAAGC TCCTGCTAAT 
AAAGCCTTTA CTGGATTCAT TGTGGAAGCA GATACCCCAG GAATTCAGAT TGGGAGAAAG 
GAATTAAACA TGGGCCAGCG ATGTTCAGAT ACTAGAGGAA TTGTCTTCGA AGATGTGAAA 
GTGCCTAAAG AAAATGTTTT AATTGGTGAC GGAGCTGGTT TCAAAGTTGC AATGGGAGCT 
TTTGATAAAA CCAGACCTGT AGTAGCTGCT GGTGCTGTTG GATTAGCACA AAGAGCTTTG 
GATGAAGCTA CCAAGTATGC CCTGGAAAGG AAAACTTTCG GAAAGCTACT TGTAGAGCAC 
CAAGCAATAT CATTTATGCT GGCTGAAATG GCAATGAAAG TTGAACTAGC TAGAATGAGT 
TACCAGAGAG CAGCTTGGGA GGTTGATTCT GGTCGTCGAA ATACCTATTA TGCTTCTATT 
GCAAAGGCAT TTGCTGGAGA TATTGCAAAT CAGTTAGCTA CTGATGCTGT GCAGATACTT 
GGAGGCAATG GATTTAATAC AGAATATCCT GTAGAAAAAC TAATGAGGGA TGCCAAAATC 
TATCAGATTT ATGAAGGTAC TTCACAAATT CAAAGACTTA TTGTAGCCCG TGAACACATT 
GACAAGTACA AAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Unveiling the Pathogenic Molecular Mechanisms of the Most Common Variant (p.K329E) in Medium-Chain Acyl-CoA Dehydrogenase Deficiency by in Vitro and in Silico Approaches.
Biochemistry55(51)7086-7098(2016 Dec)
Bonito CA,Nunes J,Leandro J,Louro F,Leandro P,Ventura FV,Guedes RC

The epidemiology of medium chain acyl-CoA dehydrogenase deficiency: an update.
Genetics in medicine : official journal of the American College of Medical Genetics8(4)205-12(2006 Apr)
Grosse SD,Khoury MJ,Greene CL,Crider KS,Pollitt RJ

Human long chain, very long chain and medium chain acyl-CoA dehydrogenases are specific for the S-enantiomer of 2- methylpentadecanoyl-CoA.
Biochimica et biophysica acta1390(3)333-8(1998 Feb)
Battaile KP,McBurney M,Van Veldhoven PP,Vockley J

Crystal structures of the wild type and the Glu376Gly/Thr255Glu mutant of human medium-chain acyl-CoA dehydrogenase: influence of the location of the catalytic base on substrate specificity.
Biochemistry35(38)12412-20(1996 Sep)
Lee HJ,Wang M,Paschke R,Nandy A,Ghisla S,Kim JJ

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ACADM cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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